JP2523074B2 - Mushroom mycelium culture method - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for artificially cultivating mushroom hyphae.

[0002]

2. Description of the Related Art Conventionally, edible mushrooms have been cultivated mainly from raw wood, but the harvest amount has been greatly affected by natural conditions such as seasons, weather, and typhoons. In recent years, even in the cultivation of shiitake mushrooms, oyster mushrooms, beech mushrooms, maitake mushrooms, yamatake mushrooms, etc., a fungus bed cultivation method has been popular in which cultivated rice is grown in polypropylene bottles or polypropylene bags using a medium in which rice bran and rice bran are mixed. It has become possible to harvest edible mushrooms steadily throughout the year without being affected by the four seasons.

Various substances (for example, pyrroloquinoline quinone, C-adenosine monophosphate (C-AMP) are used as growth promoters of mushroom hyphae for the purpose of shortening the cultivation period and increasing the yield and quality of fruiting bodies. ), Acidic protease inhibitor S-PI, cerebroside, benzoic acid, alkaloids, etc.) are added to the medium. In addition, up to now, a sugar-modified product obtained by fractionating ligninsulfonic acid (LSA) from a sulfite pulp effluent (hereinafter abbreviated as SSL) is mainly composed of shiitake fungi and mushroom mycelia belonging to wood decay fungi other than shiitake. It is known from Japanese Examined Patent Publications Sho 57-2309 and Sho 57-2310 that they have a promoting effect on growth.

[0004]

The object of the present invention is to further enhance the growth promoting effect of mushroom hyphae by using a sugar-modified product obtained by fractionating LSA from SSL as a main component to shorten the culture period. .

[0005]

[Means for Solving the Problems] The inventors of the present invention have determined that LSA has a molecular weight cut-off of 1 from the industrially manufactured and sold SSL.
Growth of mushroom mycelium of permeate ( manufactured by Sanyo Kokusaku Pulp Co., Ltd., trade name, Sunpearl CP (registered trademark) ) containing a sugar-modified product as a main component, which was obtained by separation with an ultrafiltration membrane of 20,000. The promotion effect was examined. As a result, they have found that the combined use of Sunpearl CP and an oligopeptide-rich yeast extract containing oligopeptides in an amount of 50% or more with respect to all amino acids significantly promotes the growth of mushroom hyphae, and arrived at the present invention.

[0006] Sunpearl CP is an ultrafiltration membrane of Ca-based SSL with a cut-off molecular weight of 10,000 and is ultrafiltered (concentration ratio 4 times V
/ V, constant volume ratio 1 time V / V), which is a powdered product of the permeated liquid, and its composition (% solid content) is as follows. (%) Low molecular weight ligninsulfonic acid Ca 30 Reducing sugar 34 Sugar modified product (sugar sulfonic acid, aldonic acid) 34 Inorganic salt 2

Yeast extract is Saccharomyces
ces genus, Hansenula genus, Torulopsis genus, Candida genus, etc. are obtained by autolysis, enzyme treatment or a combination of these methods. , Whose main component is oligopeptides and nucleic acid degradation products, are widely used as seasonings for foods and nutrients for culture media. The yeast extract used in the present invention exhibits a remarkable effect when the content of oligopeptides relative to all amino acids is 50% or more, and the oligopeptide content is determined by the following formula. a: Total amino acid amount obtained by total hydrolysis of yeast extract b: Free amino acid amount obtained by analyzing yeast extract as it is

[0008]

EXAMPLES The effects of the present invention will be described below with reference to examples.

Examples 1 and Comparative Examples 1 to 4 Lentinus edodes were added to the following Henneberg medium in a yeast extract high in oligopeptide (manufactured by Sanyo Kokusaku Pulp Co., Ltd., trade name SK yeast extract HUP (hereinafter abbreviated as HUP). ), Oligopeptide content 7
4.2%] to 0.5% and sulfurous acid pulp drainage powder [Sanyo Kokusaku Pulp Co., Ltd., trade name Sun Extract P201 (registered trademark)
Standard) ] or a powder of its high molecular weight ligninsulfonic acid component [Sanyo Kokusaku Pulp Co., Ltd., trade name Pearlex C]
P (registered trademark) ] or a permeate powder obtained by ultrafiltration of a sulfite pulp effluent with an ultrafiltration membrane having a molecular weight cut off of 10,000 (manufactured by Sanyo Kokusaku Pulp Co., Ltd., trade name Sunpearl CP). (Registered trademark) ], and each of them is added 1.0%, and 100 ml is added.
25 ml was dispensed into an Erlenmeyer flask and sterilized and inoculated with a fungus disk (φ4 mm). 16 in the dark at 25 ° C
After static culture for 1 day, centrifugation (10,000 rpm,
After 10 min), shiitake hyphae were filtered under reduced pressure with a glass fiber filter paper (Toyo DP70, φ25 mm) to obtain 10
The cells were treated at 5 ° C for 3 hours, and the dry cell weight was determined. Table 1 shows the results.

[0010]

[Table 1]

[0011] Henneberg Medium Composition Glucose _{50.0g KNO 3 2.0g NH 4 H 2} PO 4 2.0g KH 2 PO 4 1.0g MgSO 4 · 7H 2 O 0.5g CaCl 2 0.1g distilled water 1 L

Examples 2 to 4, Comparative Examples 5 to 7 Shiitake fungi were added to Henneberg medium by changing the addition ratio of HUP and Sunpearl CP (registered trademark) to a total of 1.0.
%, Add 25 ml to a 100 ml Erlenmeyer flask,
The sterilized one was inoculated. The culture was allowed to stand for 21 days in the dark at 25 ° C. and then centrifuged (10,000 rpm, 10 mi).
n), the shiitake hyphae were filtered under reduced pressure with a glass fiber filter paper (Toyo GB140, φ47 mm) and treated at 105 ° C. for 3 hours to determine the dry weight of the cells. The results are shown in Table 2.
Shown in

[0013]

[Table 2]

[0014] Example 5-6, San Pearl CP Comparative Example 8-13 shiitake fungus in Henneberg medium
(Registered trademark) 0.5 % and the following yeast extracts having different oligopeptide contents of 0.5% each were statically cultivated for 21 days in the dark at 25 ° C. in the same manner as in Example 2. The dry weight was determined. Table 3 shows the results.

[0015]

[Table 3]

Examples 7 to 10, Comparative Examples 14 to 15 Hericium erinaceu
m) in the following medium with HUP 1% and Sunpearl CP (registered)
(Trademark) and variously added in the range of 0.5 to 4.0%, and the added medium is dispensed into a 100 ml Erlenmeyer flask in an amount of 50 ml,
After sterilization, 2.5 ml of a full-growth shake-cultured bacterial cell was inoculated, and 30 ° C, 100 r. p. m. After culturing with shaking for 14 days, the dry cell weight was determined in the same manner as in Example 2. The results are shown in Table 4.

[0017]

[Table 4]

[0018] Medium Composition Glucose _{30.0g KH 2 PO 4 1.4g MgSO 4} · 7H 2 O 0.5g Thiamine hydrochloride _{20 mg CuSO 4 · 5H 2 O} 20 mg distilled water 1 L

[0019]

The yeast extract and the sulfite pulp effluent specified in the present invention are subjected to an ultrafiltration membrane having a molecular weight cut off of 10,000.
In by adding the permeate obtained by ultrafiltration to the medium, to improve the growth promoting effects of mushroom mycelium is obtained by successfully shortening the incubation period.

Claims (1)

(57) [Claims] 1. A sulfite pulp effluent having a molecular weight cut-off of 10,0.
A method for culturing mushroom mycelium, which comprises adding a permeate obtained by ultrafiltration with an ultrafiltration membrane of No. 00 and yeast extract containing 50% or more of oligopeptides to all amino acids to a medium.