JP2501720B2 - Air pressure gene transfer device - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION The present invention introduces a foreign gene into a monocotyledonous or dicotyledonous plant cell and an object to be treated which is a tissue thereof, expresses the foreign gene, analyzes the genetic properties of the plant, The present invention relates to a gene transfer device used for the purpose of manipulation and modification, and is also used for the object to be treated of animals.

[0002] Regarding the gene transfer, the ones that are currently achieving results are rice, wheat, asparagus, tobacco, mandarin orange,
Eggplant, carrot, oilseed rape, purple flower, mustard, Arabidopsis, carnation, cucumber,
Soybean and the like.

[0003]

2. Description of the Related Art There is an Agrobacterium method as a gene transfer method for plants, but the target of Agrobacterium infection is limited to dicotyledonous plants. Protoplasts are used in the PEG method and electroporation method. Can only be applied to species for which the regeneration system has been established,
The microinjection method is not a universal gene transfer method because it requires time and labor to operate because it targets single cells and requires technical skill.

As a universal gene transfer method, there is a gene transfer method using gunpowder, but this method generates combustion heat, smoke, and foreign substances, is harmful to gene transfer, and can be continuously operated. Was not. There is also a gene transfer method using gas pressure, but it is expensive to obtain gas even if it does not generate harmful combustion heat, smoke, and foreign substances, and depending on the high-pressure gas in the cylinder, it can be used at a constant pressure. I couldn't get it, and I couldn't operate it continuously.

Further, there is a gene transfer method utilizing air pressure,
Kensuke Chiba, one of the inventors of the present invention, issued a patent No. 1065992.
The high-pressure gas discharge valve mechanism developed as No. ² has obtained compressed air of up to 260 kg / cm ^{2 and} a shot velocity of 498 m / sec in the barrel, making it easy to use pneumatic pressure. With this method, no harmful substances are generated and it is inexpensive because it uses air pressure, but it was not possible to operate continuously because the device was used experimentally.

[0006]

The air source device generates a constant pressure and uses the air pressure to facilitate the handling and loading of projectiles and to continuously operate the air pressure gene transfer device. Is to let.

[0007]

Means for Solving the Problems As means for solving the problems of the present invention, an air source device for generating a compressed air of a constant pressure and a large number of microparticles coated with genes are attached to an enlarged head. Drive that enables centering of the cartridge by inserting and mounting the projectile in the center opening and the cartridge in the cartridge loading space, and centering the barrel cover and the bullet stop plate in the movable barrel With the mechanism,
Furthermore, a cartridge loading device for loading the cartridge while centering it in the cartridge loading space between the fixed barrel and the movable barrel by the cartridge carrying and gripping device, and the projectile is pierced into the opening of the stopper fitting and received. Piston cylinder mechanism for moving the bullet stop plate that can rotate the bullet stop plate between the receiving position of the projectile and the ejecting position of the projectile and can be positioned at each of the receiving position and the ejecting position An air pressure gene transfer device is configured with a discharging piston cylinder mechanism that discharges a shot bullet received at a position from a bullet stop plate at a discharge position.

[0008]

[Function] The bullet stop plate is automatically positioned at the receiving position, and the cartridge fitted with the firing bullet is loaded into the cartridge loading space between the fixed barrel and the movable barrel, and the barrel of the movable barrel is loaded. When the movable barrel and the barrel cover are slid by the drive mechanism in the barrel axis direction while centering between the cover and the bullet stop plate, the cartridge is held by the fixed barrel and the movable barrel, and the preparation for firing is completed. When air from the air reservoir is sent to the fixed barrel through the discharge valve mechanism, a large number of gene-coated microparticles are projected from the enlarged head of the projectile to the object to be processed, and inside the opening of the stopper metal of the bullet stop plate. If the projectile that has been received by piercing it with a bullet is taken out from the bullet stop plate, the bullet stop plate is positioned again at the catch position, the cartridge without the shot bullet is replaced with the cartridge with the shot bullet, and the above operation is repeated. Continuous It is the air pressure available gene transfer operation.

[0009]

EXAMPLE A purification workbench 2 which is reinforced by a reinforcing bone plate 1 and purified by purified air is provided on a floor (not shown). The purification workbench 2 has columns 3 on the left and right sides and on the right rear side. In this case, a total of three pieces are upright to support a ceiling frame 4, and a ceiling plate body 5 is welded to the ceiling frame 4 and further another steel plate body 6 is provided to form a two-plate ceiling. .

The upper ends of the left side plate 7 and the right side plate 8 are welded and fixed to the ceiling of the two plates together with the reinforcing plate 9, and the left side plate 7
A bottom tightening metal fitting 10 is attached to the lower end of the right side plate 8. The right side plate 8 is provided with a cutout 8'for a cartridge gripping and conveying device described later.

An air source device 11 having a cylinder body 12, a plunger 13, a plunger shaft 14, an air reservoir, a discharge valve (see FIG. 2 for details), etc., which is close to the ceiling and penetrates the right side plate 8, has a holding metal fitting 15. , 16 is suspended on the ceiling. The side of the cylinder barrel 12 opposite to the side having the plunger shaft 14 is hermetically sealed, and is received by an adjusting metal fitting 17 penetrating through the left side plate 7 and being adjustable by a nut.

On the outer circumference of the cylinder body 12, there is a gas hole fitting 18
Is attached, and a fixed barrel 19 is airtightly screwed to the tip thereof. For this fixed barrel 19, cartridge 2
The movable barrel 22 is movably supported in the axial direction of the gun cavity so as to form a cartridge loading space 21 for 0,
A centering stopper 23 for the cartridge 20 is fixed to the upper end of the movable barrel 22, and is slidably held by a barrel upper holding plate 24 made of non-rust steel fixed to the left side plate 7. An airtight box 25 is fixed to the left side plate 7, the right side plate 8 and the bottom fastening member 10 and is made of a transparent thick acrylic plate and has an opening / closing door on the front surface.
5 ′ is airtightly attached by a nut 26, and the lower end of the movable barrel 22 penetrates through the fastening metal fitting 25 ′ and faces the airtight box 25. Tightening bracket 25 'of the movable barrel 22
A collar 27 is provided at a portion corresponding to the above. When the collar 27 comes into contact with the tightening metal fitting 25 ', the movable barrel 22 is prevented from further descending.

A piston 28 is provided at the lower end of the movable barrel 22 facing the airtight box 25.
8 is surrounded by a gun cavity cover 29, the upper end and the middle part of the gun cavity cover 29 are sealed by an upper slide fitting 30 and a lower slide fitting 31, and an upper cylinder chamber is provided between these fittings 30, 31 and the piston 28. 32 and lower cylinder chamber 33
And the lower end of the barrel cover 29 is made a free end, but if it is received by a bullet stop plate 37, which will be described later, it can be used for a reaction when the movable barrel 22 is pushed up.

A gate-shaped stopper desk 34 is installed at the bottom of the airtight box 25, and an opening 35 is provided in the top plate of the stopper desk 34.
Bullet stop plate 3 to which a stopper metal fitting 36 having 6'is attached
Metal fitting 3 that serves as a positioning device so that 7 is in the receiving position
8 is fixed by a piston-cylinder mechanism for moving a stop plate, which will be described later, and at this time, the opening 36 'is aligned with the axis of the gun cavity. A discharge piston-cylinder mechanism 39 for discharging the bullets received by the stopper fitting 36 is also attached to the top plate of the stopper desk 34. A stepped frame 40 is installed in the gate-shaped stopper desk 34, and a shelf board 41 is inserted in an appropriate step. The airtight box 25 is provided with a vacuum gauge 42 and a vent hole 43 having an electromagnetic valve.

The air source device 11 will be described in detail with reference to FIG. 2. A check valve mechanism 44 is provided in the cylinder barrel 12 so as to face the plunger 13. In the check valve mechanism 44, a valve seat 46 is inserted into a recess 45, a check valve 47 which is a ball valve is in contact with the valve seat 46, and the valve seat 46 is pressed by a valve seat retainer 48. . A first guide 49 of an exhaust valve that engages with the fixed barrel 19 is fixed to the center of the cylinder barrel 12, and is fitted with the check valve mechanism 44 to form an air reservoir 53. First
The guide 49 is provided with a recess 50 on the side of the check valve mechanism 44,
The O-ring 51 is stopped by an O-ring retainer 52, and a check valve spring 53 ′ is stretched between the O-ring retainer 52 and the check valve 47.

A second guide 55 of the exhaust valve, in which the tip of the exhaust valve 54 is slidably inserted into the first guide 49 and the rear end of the exhaust valve 54 is guided, is fitted in the cylinder body 12. Has been done. A stop plate 56 is provided which is slidable in the groove 56 'along the second guide 55 and abuts against the rearmost end of the exhaust valve 54, and the stop plate 56 escapes into the groove 56' so that the discharge valve 54 retains air. A cushion 57 for receiving the rearmost end portion of the exhaust valve 54 when the compressed air of 53 is supplied to the gun cavity is fixed. A cushion 58 is provided at the other end of the second guide 55, and the end of the cylinder body 12 is sealed by a sealing member 57 '. A flange 59 is provided between the first guide 49 and the second guide 55 of the exhaust valve 54, and an exhaust valve spring 61 is stretched between the flange 59 and the second guide 55 via a bumper plate 60. There is. An O-ring 63 is enclosed by an O-ring retainer 62 on the flange 59 side of the first guide 49.

Returning to FIG. 1, a shaft pin 64 is fixed to the opposite end of the plunger 13 of the plunger shaft 14 by a nut 65. The shaft pin 64 penetrates the ceiling, penetrates one end 67 of the crankshaft 66 and the slide 68, and is fixed by a nut 69. The slide 68 is provided by a slide guide plate 71 having a holding plate 70 provided on both sides. , Cylinder body 12
Is guided in the axial direction. Therefore, the ceiling is provided with a through hole 72 for the shaft pin 64 provided therethrough. The other end 73 of the crankshaft 66 is a rotating plate 78 fixed by a knock pin 77 to a motor shaft 76 of a vertical geared brake motor 75 that obtains a rotating shaft force of 1 HP fixed to a motor base 74 fixed to the ceiling. On the other hand, it is pivotally attached by a pin 79 so as to be rotatable.

A cartridge loading device 81 having a cartridge gripping and transporting device 80 will be described with reference to FIGS.

The cartridge gripping and conveying device 80 includes a guide tube 8
1 from the position of the lower dashed line along the guide groove 83 of FIG.
It is rotated 90 ° in the horizontal direction to the position shown in the drawing and then raised.

The guide tube 82 is a guide groove 8 for guiding the ascent.
3 and the upper and lower ends are fixed by receiving brackets 84, 84,
A screw shaft 85 is provided at the center, and the upper and lower ends of the screw shaft 85 are supported by ball bearings. A pulley 86 is fixed to the lowermost end of the screw shaft 85, and the right side plate 8 has a motor mounting bracket 8
The shaft 89 of the motor 88 mounted by
0 is fixed, a rubber belt 91 is stretched over these pulleys 86 and 90, and the screw shaft 85 is attached to the motor 8
8 driven.

Referring to FIGS. 2 and 3, the details of the cartridge gripping and carrying device 80 will be described.
Is covered with a sliding body 92, and the sliding body 92 is a guide tube 8
It is slidable with respect to 2. A screw 93 is screwed onto the screw shaft 85, and a connecting pin 94 that penetrates the sliding body 92 and is guided in the guide groove 83 of the guide tube 82 is fixed to the screw 93. Has been done. Therefore, when the screw shaft 85 rotates, the screw 93 that is screwed with the screw shaft 85 is guided by the connecting pin 94 along the guide groove 83 and moves up and down, and also rotates 90 ° in the horizontal direction. That is, the cartridge gripping and transporting device 80 starts from the position of the one-dot chain line in FIG.
When it descends, it rises to the position indicated by the solid line in FIG. 1 and then stops, and when descending, it returns to the position indicated by the alternate long and short dash line in FIG. The cartridge is replaced at the position indicated by the alternate long and short dash line.

A T-shaped support base 95 is attached to the sliding body 92 and is reinforced by a reinforcing frame 96. Support base 9
5 is provided with a flange 97. This flange 97
A cylinder body 98 is fixed to. A piston 99 is provided on the cylinder body 98, a piston shaft 100 is attached to the piston 99, and the piston shaft 100 is slidable in the axial direction. At one end of the piston shaft 100, a metal fitting 102 having a square rod-shaped cross section of a cartridge gripping portion 101 is screwed and is adjustable so as to be fixed.
It is firmly fixed by 3 and guided by a guide 95 '. The other metal fitting 104 of the cartridge gripping portion 101 is pinchedly connected to the one metal fitting 102 by a pin 105, and a rib 106 is interposed between the two, so that the cartridge gripping portion 101 slightly exceeds a semicircle. Circular cartridge 2 with a semicircular gripping recess
It is always urged in the direction of holding 0.

As shown in FIGS. 2 and 3, the cartridge 20 has a cylindrical outer shape, has a constriction 107 in the central portion thereof, and O-rings 108 are embedded in both end surface portions thereof, and a central portion is formed at the center. An opening 109 is provided, and a pin wound projectile 110 having enlarged heads 111 at both ends of a cylindrical outer shape is fitted and attached to the central opening 109 at the peripheral edge of the enlarged head. The maximum degree is 260 kg / cm ²
An initial velocity of 498 m / sec is obtained by receiving the air pressure of, and the projectile 110 is pierced into the opening 36 ′ of the stopper fitting 36 after being launched and is received by the enlarged head 111 and the peripheral surface portion of the cylindrical outer shape. At that time, the peripheral surface portion has plastic deformation and elastic deformation, but has the property and strength that it is not crushed as a whole. In the state shown in FIG.
The enlarged head 111 on the lower side of 0 can be located in a state of being recessed from the end face of the cartridge 20 due to the height difference between the projectiles 110 and the cartridge 20, and is attached to the enlarged head 111 and coated with a gene. Make sure that a large number of fine particles having a diameter of 1 μm to 1.2 μm are not detached when the cartridge 20 is loaded into the cartridge loading space 21 by the cartridge loading device 81 or when the fixed barrel 19 and the movable barrel 22 hold the cartridge. is there. The fine particles are preferably chemically stable metal fine particles.

Using the cartridge gripping and conveying device 80,
When the cartridge 20 is loaded into the cartridge loading space 21 as the cartridge loading device 81, the cartridge 20 is mounted on the movable barrel 22 and the centering stopper 2 is fixed.
3 is contacted and centered. Such movement is caused by the cylinder body 98 and the piston 9 of the cartridge gripping and conveying device 80.
9, and the gripper 101 is guided by a guide 95 '. As shown in FIGS. 2 and 3, the centering stopper 23 is notched so as to be slightly recessed from the center line, and the remaining centering stopper portion is the cartridge 2
It is slightly shorter than 0 and has a cylindrical inner diameter that matches the cylindrical outer shape of the cartridge 20. As a result, the cartridge 20 is centered under the action of the cylinder body 98 and the piston 99, and the fixed barrel 19 and the movable barrel 2
2 and O-rings 10 at both ends of the cartridge 20.
Held airtight through 8. This completes the loading of the cartridge 20.

Regarding the bullet stop plate operating device 112, FIG.
This will be described in detail with reference to 4 and 5.

The elastic stopper plate 37 to which the stopper metal fitting 36 is attached is received by a metal fitting 38 serving as a positioning device so that it is in the receiving position at the position shown in the drawing, and at this time, the opening 36 'of the stopper metal fitting 36 is in the gate. Shaped stopper desk 3
4, the line is aligned with the opening 35, and when the projectile 110 is fired at a predetermined speed, the projectile 110 is not crushed and pierces into the opening 36 ', and its enlarged head 111 and the peripheral surface of the cylindrical outer shape. And then fired 110
1μ to 1.2μ diameter attached to the enlarged head 111 of
Of the particles are projected at the same speed as the initial velocity of the projectile 110 together with the genes covered, pass through the opening 36 ′ of the stopper fitting 36 and the opening 35 of the stopper desk 34, and the stepped frame 40 inside the stopper desk 34. Shelf board 41 inserted in
The gene will be introduced together with the microparticles into the object to be treated placed on.

The bullet stopping plate 37 is pivotally mounted by a pin shaft 113 so as to be rotatable between this receiving position and a discharging position for piercing the opening 36 'of the stopper fitting 36 for discharging the received bullet. Has been done. This rotation is performed by a piston-cylinder mechanism 116 for moving a stop plate, one end of which is supported by a support shaft 114 provided on the stopper desk 34 and the other end of which is supported by a support shaft 115 provided on the stop plate 37. Done,
As a result, the bullet stop plate 37 is moved from the solid line position (reception position) in FIG. 5 to the alternate long and short dash line position (discharge position), and vice versa. There is also a metal fitting 38 'that serves as a positioning device at the position indicated by the alternate long and short dash line, that is, the discharge position.
When the elastic stopper plate 37 comes into contact with 8 ', the stopper metal fitting 3
The opening 36 ′ of 6 is aligned with the discharge rod 117 of the discharge piston-cylinder mechanism 39. The metal fittings 38 and the metal fittings 38 ′ serving as positioning devices for the receiving position and the discharging position are screwed by screws 118, 118 so that they can be adjusted to the stopper desk 34.

A V-shaped groove 119 is provided as an annular centering portion surrounding the stopper fitting 36 of the elastic stopper plate 37 and corresponding to the centering portion of the knife edge 120 at the free end of the gun cavity cover 29. Yes, metal fitting 38 at the free end of the barrel cover 29
The portion that collides with the (reception position) is formed as a notch 121 according to the metal fitting 38. This notch 121 is a shot 1
It also serves as an air escape hole when 10 is fired, and also serves as a stop around the gun barrel cover 29. These form a part of the barrel centering device.

The vent hole 43 provided with an electromagnetic valve provided in the airtight box 25 serves as an air outlet hole when the inside of the airtight box 25 is evacuated, and air when the object to be processed is taken out after the gene is introduced into the object to be processed. It also serves as an introduction hole. The airtight box 25 is provided with an outlet for clean air (not shown), and after the gene introduction, for the next gene introduction, around the bullet stop plate 37, the stopper desk 34, etc. Purify.

When the fine particles are introduced into the object to be processed together with the gene, in order to prevent the object to be processed from being destroyed, the air pressure supplied to the fixed barrel 19 is adjusted or the stepped frame 4 is used.
This is dealt with by changing the position of the step of the shelf board 41 to be inserted into 0.

The projectile 110 is placed in the opening 1 of the cartridge 20.
The expansion head 1 of the projectile 110 is fitted into
If the diameter of 11 is made a little larger, finished to a predetermined size through the throttle hole, and fitted, the efficiency is improved.

Although not shown, a hand switch box is provided at the left end of the cleaning workbench 2 so that it can be moved to an arbitrary position, and each hand switch box accommodates each relay and other electric devices. The front side is provided with an energizing button, an operation start button, a work restart button in case of a power failure during work, an emergency stop button, a fuse case, etc., so that an automatic command can be issued.

There is a counter switch on the right side of the hand switch box, and the pressure of the air source device 11 is automatically adjusted by a chart showing the relationship between the pressure and the firing speed before the work is started, and the air source device is adjusted according to the adjustment. 11 is shut down. The operation order is as follows.

1. When the operation start button is pressed, the motor 88
1 is driven to rotate normally, and the cartridge gripping and conveying device 80 is shown in FIG.
It is raised from the position indicated by the one-dot chain line to the position indicated by the solid line, and is rotated 90 ° in the horizontal direction on the way to stop at a fixed position. At this time, the motor 88 is set to the reverse drive position, air is supplied to the cylinder body 98 via the solenoid valve, and the piston 99 is pushed in the direction to push out the piston shaft 100, so that the cartridge 2 sandwiched by the cartridge gripper 101.
0 is continuously pressed for 2 to 3 seconds on the centering stopper 23 in the cartridge loading space 21 to perform the work as the cartridge loading device 81.

In the meantime, air is sent to the lower cylinder chamber 33 of the gun cavity cover 29 by the solenoid valve, the gun cavity cover 29 descends, and the centering portion 12 of the gun cavity cover 29 between it and the bullet stop plate 37.
0 and the centering portion 119 of the bullet stop plate 37 perform centering of the barrel, and then the movable barrel 22 rises to move the fixed barrel 1
The cartridge 20 is sandwiched between 9 and 9 to operate as a barrel centering device.

Then, the shelf board 4 is attached to the selected step of the stepped frame 40.
When 1 is placed and the container containing the object to be processed is placed at the designated position on the shelf plate 41, the line of the gun cavity and the center of the container can be aligned, so that the airtight box 25 Close the door.

2. When the illumination button is pressed, the yellow light bulb is turned on in the airtight box 25, the solenoid valve of the ventilation hole 43 is opened, and the airtight box 25 is evacuated in about 20 seconds by a vacuum pump (not shown). When the desired vacuum is obtained while looking at the vacuum gauge 42 attached to the airtight box 25, when the illumination button is pressed again, the yellow light bulb is turned off and the vacuum pump is also stopped.

3. When you press the fire button, the air source device 11
The air reservoir 53 whose exhaust valve 54 is retracted to a predetermined pressure
Of air is supplied all at once into the fixed barrel 19, and the projectiles 110 in the cartridge 20 are launched at a predetermined speed through the barrel of the movable barrel 22 to open the stopper fitting 36.
It is received in a state of being pierced through the enlarged head of the projectile 110 and the peripheral surface of the cylindrical outer shape. At that time, the particles coated with the gene attached to the enlarged head portion 111 of the projectile 110 are introduced into the object to be processed together with the gene. The airtight box 25 is preferably transparent so that these states can be seen from the outside.

When the firing is completed, the air source device 11 starts to operate so as to generate a predetermined pressure, clean air flows into the airtight box 25, and air is supplied to the upper cylinder chamber 32 of the gun barrel cover 29 by the solenoid valve. When the movable barrel 22 starts to move down and the collar 27 comes into contact with the tightening metal fitting 25 ', the barrel cover 29 starts to move up and the centering engagement with the bullet stop plate is released.

At this time, the piston 99 of the cylinder body 98 of the cartridge loading device 81 is driven in the direction in which the piston shaft 100 is pulled in, and the cartridge gripping portion 101 holds the cartridge 20 while holding the cartridge loading space 21.
Drawn from. Next, the motor 88 is reversely driven and stopped at the position indicated by the alternate long and short dash line in FIG. The operation of each of these parts is performed with an appropriate time lag.

Further, the elastic stopper plate 37 is moved from the solid line position in FIG. 5 to the alternate long and short dash line position by the elastic stopper plate moving piston-cylinder mechanism 116 and then stops. Further, the operation of the air source device 11 for the next generation, the inflow of the clean air into the airtight box 25, and the return to the normal pressure are sequentially performed with an appropriate time lag.

4. When the eject button is pressed, the eject piston-cylinder mechanism 39 is activated and the ejector rod 1 pierced by the push rod 117 into the opening 36 ′ of the stopper fitting 36.
Discharge 10.

The operation time of the series of gene transfer devices described above is completed in less than 2 minutes, and the user can work while sitting on the chair toward the purification workbench 2, and press these four buttons to the right every time. If you arrange them from left to right, you can reduce the time and labor by pushing them sequentially, it is safe, clean, no foreign substances are generated, it has high capacity, and the experiment is reproduced by past data. You can In addition, the amount of fine particles required for one-time gene introduction is as small as 0.5 mg, there is no consumable other than the projectile 110, and the running cost is the lowest and the efficiency is high.

The hand switch box is provided with a pin lever switch and a small red button near the firing button. The pin lever switch is used only at the final launch,
The operation of the next air source device 11 is stopped.
The small red button is pressed when the operator wants to open the airtight box 25 after the operation before the launch, and when this button is pressed, clean air flows into the airtight box 25 and the door can be opened. become. After that, it is sufficient to start from the above operation 2.

[0045]

EFFECTS OF THE INVENTION (1) The air pressure gene transfer device of the present invention has a constant pressure air source device, a cartridge in which a projectile is fitted in the peripheral portion of its enlarged head in a pincushion shape, and a cartridge is loaded. Since it is composed of the cartridge loading gap, the barrel centering part, and the drive mechanism, a certain pressure can be reliably obtained, and it can be operated in a predetermined order and is safe.
It is clean, has no foreign substances, has high performance, has few consumables, and has high efficiency.

(2) Since the projectile has a pincushion shape and has enlarged heads at both ends of the cylindrical outer shape, the frictional resistance when the projectile after passing through the gun cavity is reduced, and the projectile is Can slow down.

(3) Since the projectile is stably fitted and mounted in the central opening of the cardridge by the peripheral edges of the enlarged heads at both ends thereof, the initial velocity of the projectile is secured and the projectile is Even if the cartridge is small and uncomfortable to sit in, the cartridge can be made appropriately large and comfortable to sit in.Of course, the handling of the projectile must be done while paying attention to the microparticles coated with the attached gene. But not
Since it is a projectile that is fitted and attached to the cartridge, it is handled with each cartridge and handling is easy and safe,
It can be easily loaded into the cartridge loading space formed between the fixed barrel and the movable barrel.

(4) Since the drive mechanism for sliding the movable barrel and the barrel cover in the direction of the barrel axis and the centering portion provided between the barrel cover and the bullet stop plate, the cartridge is loaded. When a cartridge fitted with a shot ammunition is loaded in the space for the cartridge and the cartridge is sandwiched between the fixed barrel and the movable barrel, the axis of the fixed barrel and the movable barrel becomes the opening axis of the stopper metal fitting on the bullet stop plate. It can be matched and the axis of the projectile can be easily matched to the bore axis.

(5) Since the fired projectile is pierced in the opening of the stopper fitting of the bullet stop plate and is received by the enlarged head of the fired bullet and the peripheral surface of the cylindrical outer shape, the enlarged head of the fired bullet is received. Almost all of the fine particles adhering to are projected through the opening, and the plastic deformation and elastic deformation of the enlarged head and the peripheral surface of the cylindrical outer shape can be used for receiving. You can take it.

(6) The cartridge loading device having the cartridge gripping and transporting device and the centering stopper for receiving the cartridge cooperate to mechanically load and unload the cartridge into the cartridge loading space.

(7) Since it has a knife-edge-shaped centering portion at the end of the barrel cover and an annular centering portion of the V-shaped groove of the bullet stop plate, the centering portion of the bullet stop plate is stable. It can also be used for reaction when pushing up the movable barrel, and overall accurate centering on the axis of the barrel is easy.

(8) Since the movable barrel and the gun barrel cover can be separated from the bullet stopping plate by contact with the driving mechanism in addition to being abutted by the driving mechanism, only the bullet stopping plate is ejected to the receiving position and the ejecting point. It can be rotated by a piston-cylinder mechanism for moving the bullet stop plate between the positions and the positioning device is provided at each of the above-mentioned positions. It's easy.

(9) The projectile shot pierced and received in the opening of the stopper fitting of the bullet stopping plate is easily discharged by the discharging piston-cylinder mechanism at the discharging position.

[Brief description of drawings]

FIG. 1 is a front view showing a part of a device of the present invention in section.

FIG. 2 is a partial vertical sectional front view showing main parts of an air source device and a cartridge loading device of the device of the present invention.

FIG. 3 is a plan view taken along line III-III and partly line AA of FIG. 2.

FIG. 4 is a partial vertical sectional front view showing a main part of the bullet stop plate operating device of the device of the present invention.

5 is a plan view excluding the gun barrel covering portion of FIG. 4. FIG.

[Explanation of symbols]

11. . . . Air source device 19. . . . Fixed barrel 20. . . . Cartridge 21. . . . Void for cartridge loading 22. . . . Movable barrel 23. . . . Centering stopper 28, 30, 31, 32, 33. . . . Drive mechanism 29. . . . Gun cavity cover 36 '. . . . . Opening 37. . . . Bullet stop plate 38, 38 '. . . . Positioning device (metal fitting) 39 Piston-cylinder mechanism for discharge 80. . . . Cartridge gripping and conveying device 81. . . . Cartridge loading device 108. . . . O-ring 109. . . . Opening 110. . . . Fire bullet 111. . Enlarged head 116 Piston-cylinder mechanism for moving bullet stop plate 119. . . . Centering part of bullet retaining plate 120. . . . Centering part of the gun barrel cover

 ─────────────────────────────────────────────────── ─── Continuation of the front page (56) Reference JP-A-3-76568 (JP, A) JP-A-3-251187 (JP, A) JP-A-4-237485 (JP, A) JP-A-4- 237486 (JP, A) JP-A-5-192127 (JP, A) Special Table 5-504061 (JP, A)

Claims (5)

(57) [Claims] 1. An air source device (11) for generating compressed air of a constant pressure, and a fixed barrel (1) to which compressed air of the air source device is supplied.
9), a movable barrel (22) movably supported in the axial direction of the fixed barrel, and a cartridge loading space (21) formed between the fixed barrel (19) and the movable barrel (22). And a projectile (110) having enlarged heads (111) on both ends of a cylindrical outer shape, and a large number of gene-coated microparticles attached to one of the enlarged heads. A cartridge (20) fitted and mounted in a central opening (109) of a cartridge (20) having a cylindrical outer shape and O-rings (108) on both end surfaces by a peripheral portion of the portion.
And a centering portion of the barrel which is movably supported in the axial direction of the barrel relative to the movable barrel (22) at the end opposite to the end of the movable barrel (22) toward the cartridge loading space (21). And a drive mechanism (28, 30, 31, 32, 3) for sliding the movable barrel (22) and the barrel cover (29) in the barrel axis direction with respect to each other.
3) and a large number of microparticles attached to the enlarged head of the projectile, which are received by the enlarged head and the peripheral surface of the cylindrical outer shape, are projected together with the gene so that the launched projectile (110) is pierced. A cartridge stopper plate (37) having a stopper fitting (36) having an opening (36 ') for opening and a centering portion (119) corresponding to the centering portion (120) of the gun cavity cover (29), and a cartridge. The cartridge (2
0) is loaded, the drive mechanism is operated to center the barrel by the centering part (120) of the gun cavity cover (29) and the centering part (119) of the bullet stop plate (37). The gene transfer device utilizing air pressure, comprising: holding the cartridge (20) between the fixed barrel (19) and the movable barrel (22). 2. A cartridge loading device (81) having a cartridge gripping and transporting device (80) for gripping a cartridge (20) and loading the cartridge in a cartridge loading space (21), and the cartridge loading device (80). When the cartridge (20) is loaded by means of 81, the cartridge (20) is received and the central opening (108) of the cartridge (20) is fitted to the barrel axis of the fixed barrel (19) and the movable barrel (22). )
A centering stopper (23) attached to the movable barrel (22), the centering stopper (23) having an inner shape that matches a part of the outer shape of the cartridge, so as to align the axis of the cartridge. Item 1. A pneumatic-introduced gene transfer device according to item 1. 3. The barrel cover (29) is cylindrical and has a knife-edge-shaped centering portion (120) at its end, and the bullet stop plate (37) is the knife-edge-shaped centering portion. The air pressure gene transfer device according to claim 1, which has an annular centering portion (119) of a V-shaped groove at a corresponding position. 4. An opening through which a large number of fine particles adhering to the expanded head of the projectile are projected together with genes by receiving the launched projectile (110) at the expanded head and the peripheral surface of the cylindrical outer shape. An elastic stopper plate (37) having a stopper metal fitting (36) having a stopper metal (36 ') is provided around the stopper metal fitting (3).
The position of the opening (36 ') of 6') can be rotated between the receiving position and the discharging position of the received projectile by the bullet stop plate moving piston-cylinder mechanism (116), and the receiving position The air pressure gene transfer device according to claim 1, further comprising a positioning device (38, 38 ') for positioning the elastic stopper plate (37) at the discharge position. 5. The projectile (110) is pierced into the opening (36 ') of the stopper fitting (36) of the bullet stop plate (37) and is received by the enlarged head and the peripheral surface of the cylindrical outer shape. Then, the elastic stopper plate (37) is received by the elastic stopper plate moving piston-cylinder mechanism (116) with respect to the positioning device (38 ') at the discharge position, and at this position, the stopper plate of the elastic stopper plate (37) is held. Opening of fitting (36) (3
6 ') A projectile (110) pierced in and received
Is discharged by a discharging piston-cylinder mechanism (39), The air pressure gene transfer device according to claim 1, characterized in that.