JP2025511790A - ｉＰＳＣ由来ＨＳＣ及び子孫の大規模製造 - Google Patents

ｉＰＳＣ由来ＨＳＣ及び子孫の大規模製造 Download PDF

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JP2025511790A
JP2025511790A JP2024559279A JP2024559279A JP2025511790A JP 2025511790 A JP2025511790 A JP 2025511790A JP 2024559279 A JP2024559279 A JP 2024559279A JP 2024559279 A JP2024559279 A JP 2024559279A JP 2025511790 A JP2025511790 A JP 2025511790A
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cells
cell
hscs
culture medium
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JP2025511790A5 (https=
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エレナ・マッツァ
ベンジャミン・デムーラン
エヴァ・ダミコ
ヴィルジニー・クロディーヌ・セヴリーヌ・シュタイゲルバウト
ステファン・ロバート・ブラーム
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セリントラ・セラピューティクス・エスア
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/145—Thrombopoietin [TPO]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/165—Vascular endothelial growth factor [VEGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2303—Interleukin-3 (IL-3)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2306—Interleukin-6 (IL-6)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/26—Flt-3 ligand (CD135L, flk-2 ligand)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

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  • Health & Medical Sciences (AREA)
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  • Life Sciences & Earth Sciences (AREA)
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  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2024559279A 2022-04-05 2023-04-05 ｉＰＳＣ由来ＨＳＣ及び子孫の大規模製造 Pending JP2025511790A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP22166737 2022-04-05
EP22166737.1 2022-04-05
EP22166735.5 2022-04-05
EP22166735 2022-04-05
PCT/EP2023/059036 WO2023194488A1 (en) 2022-04-05 2023-04-05 Large scale manufacturing of ipsc derived hsc and progeny

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JP2025511790A true JP2025511790A (ja) 2025-04-16
JP2025511790A5 JP2025511790A5 (https=) 2026-04-15

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US (1) US20250223558A1 (https=)
EP (1) EP4504915A1 (https=)
JP (1) JP2025511790A (https=)
CA (1) CA3247482A1 (https=)
WO (1) WO2023194488A1 (https=)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4574969A1 (en) * 2023-12-22 2025-06-25 Sartorius Stedim Biotech GmbH A method of producing differentiated cells from pluripotent stem cells, cell populations obtained by the method, corresponding pharmaceutical compositions and a system for producing differentiated cells from pluripotent stem cells

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2019368313A1 (en) * 2018-10-24 2021-05-20 Hebecell Corporation Methods and systems for manufacturing hematopoietic lineage cells
EP4185687A1 (en) 2020-07-23 2023-05-31 Ncardia B.V. Closed manufacturing processes for large scale manufacturing of pluripotent stem cell derived cells

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CA3247482A1 (en) 2023-10-12
WO2023194488A1 (en) 2023-10-12
US20250223558A1 (en) 2025-07-10
EP4504915A1 (en) 2025-02-12

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