JP2025506699A - 低減された配列特異的エラーを用いて操作されたポリメラーゼ - Google Patents

低減された配列特異的エラーを用いて操作されたポリメラーゼ Download PDF

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JP2025506699A
JP2025506699A JP2024548587A JP2024548587A JP2025506699A JP 2025506699 A JP2025506699 A JP 2025506699A JP 2024548587 A JP2024548587 A JP 2024548587A JP 2024548587 A JP2024548587 A JP 2024548587A JP 2025506699 A JP2025506699 A JP 2025506699A
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polymerase
seq
nucleotide
polymerases
nucleic acid
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JP2025506699A5 (https=
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イェンドリク ブースビー－ヘンチェル，
テイラー ロペス，
マイケル クライン，
バージニア サーデ，
マシュー ケリンガー，
マーク アンブローソ，
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エレメント バイオサイエンシズ，インコーポレイティド
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Publication of JP2025506699A publication Critical patent/JP2025506699A/ja
Publication of JP2025506699A5 publication Critical patent/JP2025506699A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2024548587A 2022-02-18 2023-02-17 低減された配列特異的エラーを用いて操作されたポリメラーゼ Pending JP2025506699A (ja)

Applications Claiming Priority (13)

Application Number Priority Date Filing Date Title
US202263311939P 2022-02-18 2022-02-18
US63/311,939 2022-02-18
US202263343036P 2022-05-17 2022-05-17
US63/343,036 2022-05-17
US202363479490P 2023-01-11 2023-01-11
US63/479,490 2023-01-11
US18/160,951 2023-01-27
US18/160,952 2023-01-27
US18/160,952 US12163163B2 (en) 2022-02-18 2023-01-27 Engineered polymerases with reduced sequence-specific errors
US18/160,951 US12006518B2 (en) 2022-02-18 2023-01-27 Engineered polymerases with reduced sequence-specific errors
US18/160,947 US11788075B2 (en) 2022-02-18 2023-01-27 Engineered polymerases with reduced sequence-specific errors
US18/160,947 2023-01-27
PCT/US2023/062874 WO2023159219A2 (en) 2022-02-18 2023-02-17 Engineered polymerases with reduced sequence-specific errors

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JP2025506699A true JP2025506699A (ja) 2025-03-13
JP2025506699A5 JP2025506699A5 (https=) 2026-02-26

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US (4) US11788075B2 (https=)
EP (1) EP4479528A4 (https=)
JP (1) JP2025506699A (https=)
KR (1) KR20240158392A (https=)
CN (1) CN119032166A (https=)
AU (1) AU2023221935A1 (https=)
CA (1) CA3244516A1 (https=)
IL (1) IL315002A (https=)
WO (1) WO2023159219A2 (https=)

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US10704094B1 (en) 2018-11-14 2020-07-07 Element Biosciences, Inc. Multipart reagents having increased avidity for polymerase binding
US12391929B2 (en) 2019-05-24 2025-08-19 Element Biosciences, Inc. Polymerase-nucleotide conjugates for sequencing by trapping
US12359193B2 (en) 2022-03-04 2025-07-15 Element Biosciences, Inc. Single-stranded splint strands and methods of use
US12371743B2 (en) 2022-03-04 2025-07-29 Element Biosciences, Inc. Double-stranded splint adaptors and methods of use
AU2022316142A1 (en) 2021-07-21 2024-02-22 Element Biosciences, Inc. Optical systems for nucleic acid sequencing and methods thereof
US12606819B2 (en) 2022-07-05 2026-04-21 Element Biosciences, Inc. PCR-free library preparation using double-stranded splint adaptors and methods of use
EP4573213A1 (en) 2022-08-15 2025-06-25 Element Biosciences, Inc. Spatially resolved surface capture of nucleic acids
AU2023343187A1 (en) 2022-09-12 2025-03-13 Element Biosciences, Inc. Double-stranded splint adaptors with universal long splint strands and methods of use
WO2025024465A1 (en) 2023-07-24 2025-01-30 Element Biosciences, Inc. On-support circularization and amplification for generating immobilized nucleic acid concatemer molecules
WO2025147667A1 (en) * 2024-01-05 2025-07-10 Element Biosciences, Inc. Target probe complexes and analyte detection complexes for in situ detecting and identifying target analytes
WO2025163526A1 (en) 2024-01-30 2025-08-07 Element Biosciences, Inc. Method for improving nucleic acid sequencing quality by eliminating nucleic acids with deaminated bases from library and method for sequencing in which complexes of primers, polymerases and labelled probes are bound to concatemers
WO2025240909A1 (en) 2024-05-17 2025-11-20 Element Biosciences, Inc. Hybridization-based capture and enrichment of target nucleic acid sequences
CN121065140B (zh) * 2025-11-06 2026-01-30 中国人民解放军军事科学院军事医学研究院 一种高保真dna聚合酶及其制备方法和应用

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DE60027040T2 (de) * 1999-10-29 2006-11-23 Stratagene California, La Jolla Zusammensetzungen und methoden zur verwendung von dna polymerasen
US8268605B2 (en) 1999-10-29 2012-09-18 Agilent Technologies, Inc. Compositions and methods utilizing DNA polymerases
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
GB0507835D0 (en) 2005-04-18 2005-05-25 Solexa Ltd Method and device for nucleic acid sequencing using a planar wave guide
GB0804721D0 (en) 2008-03-14 2008-04-16 Genesys Ltd Enzyme
WO2009145828A2 (en) 2008-03-31 2009-12-03 Pacific Biosciences Of California, Inc. Two slow-step polymerase enzyme systems and methods
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Publication number Publication date
WO2023159219A3 (en) 2023-10-05
AU2023221935A1 (en) 2024-09-05
US20230265400A1 (en) 2023-08-24
US12163163B2 (en) 2024-12-10
WO2023159219A2 (en) 2023-08-24
KR20240158392A (ko) 2024-11-04
US20240287482A1 (en) 2024-08-29
US20230265402A1 (en) 2023-08-24
US11788075B2 (en) 2023-10-17
CA3244516A1 (en) 2023-08-24
US20230265401A1 (en) 2023-08-24
US12006518B2 (en) 2024-06-11
EP4479528A2 (en) 2024-12-25
EP4479528A4 (en) 2026-04-08
IL315002A (en) 2024-10-01
CN119032166A (zh) 2024-11-26

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