JP2025502811A - 単一ワークフローにおける遺伝子情報及びエピジェネティック情報の検出 - Google Patents

単一ワークフローにおける遺伝子情報及びエピジェネティック情報の検出 Download PDF

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JP2025502811A
JP2025502811A JP2024539661A JP2024539661A JP2025502811A JP 2025502811 A JP2025502811 A JP 2025502811A JP 2024539661 A JP2024539661 A JP 2024539661A JP 2024539661 A JP2024539661 A JP 2024539661A JP 2025502811 A JP2025502811 A JP 2025502811A
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strands
nucleic acid
stranded dna
cytosine
dna fragments
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JP2025502811A5 (enExample
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イェーシュン ワン，
ニコール ジャシンダ ランバート，
ベッキー ウォン，
ユイ チョン，
ハリー ウッド，
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ファウンデーション・メディシン・インコーポレイテッド
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
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  • Molecular Biology (AREA)
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  • Biochemistry (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2024539661A 2021-12-29 2022-12-28 単一ワークフローにおける遺伝子情報及びエピジェネティック情報の検出 Pending JP2025502811A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163294640P 2021-12-29 2021-12-29
US63/294,640 2021-12-29
PCT/US2022/082476 WO2023129965A2 (en) 2021-12-29 2022-12-28 Detection of genetic and epigenetic information in a single workflow

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JP2025502811A true JP2025502811A (ja) 2025-01-28
JP2025502811A5 JP2025502811A5 (enExample) 2025-12-16

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US (1) US20250051840A1 (enExample)
EP (1) EP4457357A4 (enExample)
JP (1) JP2025502811A (enExample)
CN (1) CN118541493A (enExample)
WO (1) WO2023129965A2 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12509733B1 (en) 2025-04-07 2025-12-30 Inivata Ltd. Methods and systems for detecting a primary disease

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025104430A1 (en) * 2023-11-14 2025-05-22 Tagomics Ltd Profiling method for determining epigenetic modifications
WO2025128727A1 (en) * 2023-12-12 2025-06-19 Foundation Medicine, Inc. Methods and systems for preparing a sequencing library
WO2025160495A1 (en) * 2024-01-26 2025-07-31 Cornell University Hematopoietic stem cells and uses thereof in treating cancer
WO2025235365A1 (en) * 2024-05-08 2025-11-13 Freenome Holdings, Inc. Methods and systems for improved methylation sequencing
WO2026022118A1 (en) 2024-07-23 2026-01-29 Qiagen Gmbh Method of detecting methylated nucleic acids
WO2026043738A1 (en) * 2024-08-20 2026-02-26 Natera, Inc. Improved method for methylation biomarker generation and analysis

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009132315A1 (en) * 2008-04-24 2009-10-29 Life Technologies Corporation Method of sequencing and mapping target nucleic acids
WO2010048337A2 (en) * 2008-10-22 2010-04-29 Illumina, Inc. Preservation of information related to genomic dna methylation
US20150011396A1 (en) * 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
NZ765943A (en) * 2018-01-08 2022-10-28 Ludwig Inst For Cancer Res Ltd Bisulfite-free, base-resolution identification of cytosine modifications
CA3225385A1 (en) * 2021-07-12 2023-01-19 The Trustees Of The University Of Pennsylvania Modified adapters for enzymatic dna deamination and methods of use thereof for epigenetic sequencing of free and immobilized dna

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12509733B1 (en) 2025-04-07 2025-12-30 Inivata Ltd. Methods and systems for detecting a primary disease

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CN118541493A (zh) 2024-08-23
US20250051840A1 (en) 2025-02-13
EP4457357A2 (en) 2024-11-06
EP4457357A4 (en) 2026-01-07
WO2023129965A3 (en) 2023-10-05
WO2023129965A2 (en) 2023-07-06

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