JP2024543137A - 二本鎖ｄｎａデアミナーゼ - Google Patents

二本鎖ｄｎａデアミナーゼ Download PDF

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JP2024543137A
JP2024543137A JP2024531071A JP2024531071A JP2024543137A JP 2024543137 A JP2024543137 A JP 2024543137A JP 2024531071 A JP2024531071 A JP 2024531071A JP 2024531071 A JP2024531071 A JP 2024531071A JP 2024543137 A JP2024543137 A JP 2024543137A
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Japan
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double
stranded dna
deaminase
dna
seq
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Pending
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JP2024531071A
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Japanese (ja)
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JP2024543137A5 (https=
Inventor
バイシビラ，ロムアルダス
ジョンソン，ショーン・アール
サン，ジーイー
エバンス，トーマス・シー
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ニユー・イングランド・バイオレイブス・インコーポレイテツド
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Publication of JP2024543137A publication Critical patent/JP2024543137A/ja
Publication of JP2024543137A5 publication Critical patent/JP2024543137A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04001—Cytosine deaminase (3.5.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04005—Cytidine deaminase (3.5.4.5)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2024531071A 2021-11-24 2022-11-22 二本鎖ｄｎａデアミナーゼ Pending JP2024543137A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202163264513P 2021-11-24 2021-11-24
US63/264,513 2021-11-24
US18/058,115 2022-11-22
US18/058,115 US20230257730A1 (en) 2021-11-24 2022-11-22 Double-Stranded DNA Deaminases
PCT/US2022/080345 WO2023097226A2 (en) 2021-11-24 2022-11-22 Double-stranded dna deaminases

Publications (2)

Publication Number Publication Date
JP2024543137A true JP2024543137A (ja) 2024-11-19
JP2024543137A5 JP2024543137A5 (https=) 2025-12-02

Family

ID=84981122

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2024531071A Pending JP2024543137A (ja) 2021-11-24 2022-11-22 二本鎖ｄｎａデアミナーゼ

Country Status (6)

Country Link
US (1) US20230257730A1 (https=)
JP (1) JP2024543137A (https=)
KR (1) KR20240107347A (https=)
AU (1) AU2022396419A1 (https=)
CA (1) CA3236352A1 (https=)
WO (2) WO2023097226A2 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023097226A2 (en) * 2021-11-24 2023-06-01 New England Biolabs, Inc. Double-stranded dna deaminases
EP4540415A1 (en) 2022-06-14 2025-04-23 New England Biolabs, Inc. Methods and compositions for the simultaneous identification and mapping of dna methylation
EP4705508A1 (en) 2023-05-03 2026-03-11 Guardant Health, Inc. Methods for analysis of dna methylation
AU2024355570A1 (en) 2023-10-05 2026-04-16 New England Biolabs, Inc. Methyl cytosine-selective deaminases and uses thereof
WO2025137453A1 (en) 2023-12-20 2025-06-26 New England Biolabs, Inc. Compositions, methods, kits, and instruments for analyzing rna structure

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60212642T2 (de) 2001-04-10 2007-06-14 ECOLE POLYTECHNIQUE FéDéRALE DE LAUSANNE Verfahren zur verwendung von o6-alkylguanin-dna-alkyltransferasen
EP1720981A2 (en) 2004-03-02 2006-11-15 EPFL Ecole Polytechnique Fédérale de Lausanne Mutants of o6-alkylguanine-dna alkyltransferase
US9963687B2 (en) 2014-08-27 2018-05-08 New England Biolabs, Inc. Fusion polymerase and method for using the same
US20250011748A1 (en) * 2020-01-28 2025-01-09 The Broad Institute, Inc. Base editors, compositions, and methods for modifying the mitochondrial genome
WO2022212584A1 (en) * 2021-04-01 2022-10-06 University Of Washington Bacterial dna cytosine deaminases for mapping dna methylation sites
WO2023097226A2 (en) * 2021-11-24 2023-06-01 New England Biolabs, Inc. Double-stranded dna deaminases

Also Published As

Publication number Publication date
WO2023097226A3 (en) 2023-07-20
WO2023097226A2 (en) 2023-06-01
US20230257730A1 (en) 2023-08-17
AU2022396419A1 (en) 2024-05-23
CA3236352A1 (en) 2023-06-01
WO2024112441A1 (en) 2024-05-30
KR20240107347A (ko) 2024-07-09

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