CA3236352A1 - Double-stranded dna deaminases - Google Patents
Double-stranded dna deaminases Download PDFInfo
- Publication number
- CA3236352A1 CA3236352A1 CA3236352A CA3236352A CA3236352A1 CA 3236352 A1 CA3236352 A1 CA 3236352A1 CA 3236352 A CA3236352 A CA 3236352A CA 3236352 A CA3236352 A CA 3236352A CA 3236352 A1 CA3236352 A1 CA 3236352A1
- Authority
- CA
- Canada
- Prior art keywords
- double
- stranded dna
- seq
- deaminase
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
- C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
- C12Y305/04001—Cytosine deaminase (3.5.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
- C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
- C12Y305/04005—Cytidine deaminase (3.5.4.5)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163264513P | 2021-11-24 | 2021-11-24 | |
| US63/264,513 | 2021-11-24 | ||
| US18/058,115 | 2022-11-22 | ||
| US18/058,115 US20230257730A1 (en) | 2021-11-24 | 2022-11-22 | Double-Stranded DNA Deaminases |
| PCT/US2022/080345 WO2023097226A2 (en) | 2021-11-24 | 2022-11-22 | Double-stranded dna deaminases |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3236352A1 true CA3236352A1 (en) | 2023-06-01 |
Family
ID=84981122
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3236352A Pending CA3236352A1 (en) | 2021-11-24 | 2022-11-22 | Double-stranded dna deaminases |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20230257730A1 (https=) |
| JP (1) | JP2024543137A (https=) |
| KR (1) | KR20240107347A (https=) |
| AU (1) | AU2022396419A1 (https=) |
| CA (1) | CA3236352A1 (https=) |
| WO (2) | WO2023097226A2 (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023097226A2 (en) * | 2021-11-24 | 2023-06-01 | New England Biolabs, Inc. | Double-stranded dna deaminases |
| EP4540415A1 (en) | 2022-06-14 | 2025-04-23 | New England Biolabs, Inc. | Methods and compositions for the simultaneous identification and mapping of dna methylation |
| EP4705508A1 (en) | 2023-05-03 | 2026-03-11 | Guardant Health, Inc. | Methods for analysis of dna methylation |
| AU2024355570A1 (en) | 2023-10-05 | 2026-04-16 | New England Biolabs, Inc. | Methyl cytosine-selective deaminases and uses thereof |
| WO2025137453A1 (en) | 2023-12-20 | 2025-06-26 | New England Biolabs, Inc. | Compositions, methods, kits, and instruments for analyzing rna structure |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE60212642T2 (de) | 2001-04-10 | 2007-06-14 | ECOLE POLYTECHNIQUE FéDéRALE DE LAUSANNE | Verfahren zur verwendung von o6-alkylguanin-dna-alkyltransferasen |
| EP1720981A2 (en) | 2004-03-02 | 2006-11-15 | EPFL Ecole Polytechnique Fédérale de Lausanne | Mutants of o6-alkylguanine-dna alkyltransferase |
| US9963687B2 (en) | 2014-08-27 | 2018-05-08 | New England Biolabs, Inc. | Fusion polymerase and method for using the same |
| US20250011748A1 (en) * | 2020-01-28 | 2025-01-09 | The Broad Institute, Inc. | Base editors, compositions, and methods for modifying the mitochondrial genome |
| WO2022212584A1 (en) * | 2021-04-01 | 2022-10-06 | University Of Washington | Bacterial dna cytosine deaminases for mapping dna methylation sites |
| WO2023097226A2 (en) * | 2021-11-24 | 2023-06-01 | New England Biolabs, Inc. | Double-stranded dna deaminases |
-
2022
- 2022-11-22 WO PCT/US2022/080345 patent/WO2023097226A2/en not_active Ceased
- 2022-11-22 US US18/058,115 patent/US20230257730A1/en active Pending
- 2022-11-22 CA CA3236352A patent/CA3236352A1/en active Pending
- 2022-11-22 KR KR1020247020503A patent/KR20240107347A/ko active Pending
- 2022-11-22 JP JP2024531071A patent/JP2024543137A/ja active Pending
- 2022-11-22 AU AU2022396419A patent/AU2022396419A1/en active Pending
-
2023
- 2023-05-24 WO PCT/US2023/067416 patent/WO2024112441A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2023097226A3 (en) | 2023-07-20 |
| JP2024543137A (ja) | 2024-11-19 |
| WO2023097226A2 (en) | 2023-06-01 |
| US20230257730A1 (en) | 2023-08-17 |
| AU2022396419A1 (en) | 2024-05-23 |
| WO2024112441A1 (en) | 2024-05-30 |
| KR20240107347A (ko) | 2024-07-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| P13 | Application amended |
Free format text: ST27 STATUS EVENT CODE: A-1-1-P10-P13-P146 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: BSL VERIFIED - NO DEFECTS Effective date: 20241009 |
|
| MFA | Maintenance fee for application paid |
Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 2ND ANNIV.) - STANDARD Year of fee payment: 2 |
|
| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20241127 |
|
| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT DETERMINED COMPLIANT Effective date: 20241127 |
|
| W00 | Other event occurred |
Free format text: ST27 STATUS EVENT CODE: A-1-1-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT Effective date: 20241127 |