JP2024521142A - 次世代体積インサイチュ配列決定 - Google Patents

次世代体積インサイチュ配列決定 Download PDF

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Publication number
JP2024521142A
JP2024521142A JP2023572225A JP2023572225A JP2024521142A JP 2024521142 A JP2024521142 A JP 2024521142A JP 2023572225 A JP2023572225 A JP 2023572225A JP 2023572225 A JP2023572225 A JP 2023572225A JP 2024521142 A JP2024521142 A JP 2024521142A
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Japan
Prior art keywords
oligonucleotide
sequence
nucleic acid
probe
complementary
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Pending
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JP2023572225A
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English (en)
Japanese (ja)
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JP2024521142A5 (OSRAM
Inventor
エー ダイセロス，カール
ビー リッチマン，イーサン
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Leland Stanford Junior University
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Leland Stanford Junior University
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Publication of JP2024521142A publication Critical patent/JP2024521142A/ja
Publication of JP2024521142A5 publication Critical patent/JP2024521142A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Photoreceptors In Electrophotography (AREA)
  • Particle Accelerators (AREA)
JP2023572225A 2021-05-21 2022-05-20 次世代体積インサイチュ配列決定 Pending JP2024521142A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163191455P 2021-05-21 2021-05-21
US63/191,455 2021-05-21
PCT/US2022/030370 WO2022246275A1 (en) 2021-05-21 2022-05-20 Next-generation volumetric in situ sequencing

Publications (2)

Publication Number Publication Date
JP2024521142A true JP2024521142A (ja) 2024-05-28
JP2024521142A5 JP2024521142A5 (OSRAM) 2025-05-23

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Family Applications (1)

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JP2023572225A Pending JP2024521142A (ja) 2021-05-21 2022-05-20 次世代体積インサイチュ配列決定

Country Status (6)

Country Link
US (1) US20240263228A1 (OSRAM)
EP (1) EP4352254A4 (OSRAM)
JP (1) JP2024521142A (OSRAM)
AU (1) AU2022276537A1 (OSRAM)
CA (1) CA3220740A1 (OSRAM)
WO (1) WO2022246275A1 (OSRAM)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12359253B2 (en) 2018-04-09 2025-07-15 The Board Of Trustees Of The Leland Stanford Junior University Method of in situ gene sequencing
CN119013416A (zh) * 2022-02-17 2024-11-22 维真公司 在聚合物基质中锚定片段化核酸靶标以用于成像的方法
CN117343993A (zh) * 2023-12-01 2024-01-05 中国科学院遗传与发育生物学研究所 原位检测样品中的靶核酸序列的方法
WO2025189030A1 (en) 2024-03-07 2025-09-12 Stellaromics, Inc. Methods and systems for volumetric imaging

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2011274642B2 (en) * 2010-07-09 2015-05-21 Cergentis B.V. 3-d genomic region of interest sequencing strategies
US10982271B2 (en) * 2011-01-31 2021-04-20 Roche Sequencing Solutions, Inc. Methods of identifying multiple epitopes in cells
WO2014030066A2 (en) * 2012-08-22 2014-02-27 Bernitz Mats Nilsson Methods for identifying nucleic acid sequences
CN116242687A (zh) * 2016-11-08 2023-06-09 哈佛学院院长及董事 使用merfish、扩展显微术和相关技术进行多重成像
WO2018136856A1 (en) * 2017-01-23 2018-07-26 Massachusetts Institute Of Technology Multiplexed signal amplified fish via splinted ligation amplification and sequencing
WO2019222284A1 (en) * 2018-05-14 2019-11-21 The Broad Institute, Inc. In situ cell screening methods and systems

Also Published As

Publication number Publication date
US20240263228A1 (en) 2024-08-08
EP4352254A1 (en) 2024-04-17
AU2022276537A1 (en) 2023-12-07
CA3220740A1 (en) 2022-11-24
EP4352254A4 (en) 2025-04-16
WO2022246275A1 (en) 2022-11-24

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