JP2024518437A - 細胞以下の分解能での時空間分解トランスクリプトミクス - Google Patents
細胞以下の分解能での時空間分解トランスクリプトミクス Download PDFInfo
- Publication number
- JP2024518437A JP2024518437A JP2023568525A JP2023568525A JP2024518437A JP 2024518437 A JP2024518437 A JP 2024518437A JP 2023568525 A JP2023568525 A JP 2023568525A JP 2023568525 A JP2023568525 A JP 2023568525A JP 2024518437 A JP2024518437 A JP 2024518437A
- Authority
- JP
- Japan
- Prior art keywords
- oligonucleotide probe
- oligonucleotide
- disease
- nucleic acid
- barcode sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2523/00—Reactions characterised by treatment of reaction samples
- C12Q2523/10—Characterised by chemical treatment
- C12Q2523/109—Characterised by chemical treatment chemical ligation between nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/117—Modifications characterised by incorporating modified base
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/30—Oligonucleotides characterised by their secondary structure
- C12Q2525/307—Circular oligonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/125—Rolling circle
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/118—Prognosis of disease development
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/136—Screening for pharmacological compounds
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Physics & Mathematics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Diaphragms For Electromechanical Transducers (AREA)
- Paper (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163185511P | 2021-05-07 | 2021-05-07 | |
| US63/185,511 | 2021-05-07 | ||
| US202263314873P | 2022-02-28 | 2022-02-28 | |
| US63/314,873 | 2022-02-28 | ||
| PCT/US2022/028012 WO2022236011A1 (en) | 2021-05-07 | 2022-05-06 | Spatiotemporally resolved transcriptomics at subcellular resolution |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2024518437A true JP2024518437A (ja) | 2024-05-01 |
| JP2024518437A5 JP2024518437A5 (https=) | 2025-05-15 |
Family
ID=81846204
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023568525A Pending JP2024518437A (ja) | 2021-05-07 | 2022-05-06 | 細胞以下の分解能での時空間分解トランスクリプトミクス |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20240254545A1 (https=) |
| EP (1) | EP4334471A1 (https=) |
| JP (1) | JP2024518437A (https=) |
| KR (1) | KR20240005872A (https=) |
| AU (1) | AU2022269663A1 (https=) |
| CA (1) | CA3218089A1 (https=) |
| IL (1) | IL308267A (https=) |
| WO (1) | WO2022236011A1 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7783672B1 (ja) * | 2025-02-03 | 2025-12-10 | バイオ - セラピー、フィジオロジカル システムズ フォー ヘルス ソシエタ ペル アチオニ | 治療用又は有益な製品の生物学的効果の発現、持続及び消失を特徴付けるための方法 |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7502787B2 (ja) | 2018-04-09 | 2024-06-19 | ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー | インサイチュ遺伝子配列決定の方法 |
| US12467081B2 (en) | 2020-11-23 | 2025-11-11 | Pleno, Inc. | Encoded endonuclease assays |
| WO2022246242A1 (en) * | 2021-05-21 | 2022-11-24 | The Board Of Trustees Of The Leland Stanford Junior University | Scalable distributed processing software for next-generation in situ sequencing |
| WO2024167929A1 (en) * | 2023-02-06 | 2024-08-15 | Applied Materials, Inc. | Target probes with landmark sequences for localizing gene transcripts by fluorescence in situ hybridization |
| WO2025059443A1 (en) | 2023-09-15 | 2025-03-20 | The Broad Institute, Inc. | Untargeted multiplexed in situ rna profiling and the uses and means therefor |
| WO2025136916A1 (en) | 2023-12-18 | 2025-06-26 | The Board Institute, Inc. | Thick tissue transcriptomics and translatomics |
| WO2025189030A1 (en) | 2024-03-07 | 2025-09-12 | Stellaromics, Inc. | Methods and systems for volumetric imaging |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10434197B2 (en) | 2010-07-23 | 2019-10-08 | University Of Delaware | Tetrazine-trans-cyclooctene ligation for the rapid construction of radionuclide labeled probes |
| JP7502787B2 (ja) | 2018-04-09 | 2024-06-19 | ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー | インサイチュ遺伝子配列決定の方法 |
-
2022
- 2022-05-06 AU AU2022269663A patent/AU2022269663A1/en active Pending
- 2022-05-06 JP JP2023568525A patent/JP2024518437A/ja active Pending
- 2022-05-06 IL IL308267A patent/IL308267A/en unknown
- 2022-05-06 KR KR1020237041959A patent/KR20240005872A/ko active Pending
- 2022-05-06 EP EP22725647.6A patent/EP4334471A1/en active Pending
- 2022-05-06 WO PCT/US2022/028012 patent/WO2022236011A1/en not_active Ceased
- 2022-05-06 CA CA3218089A patent/CA3218089A1/en active Pending
- 2022-05-06 US US18/559,322 patent/US20240254545A1/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7783672B1 (ja) * | 2025-02-03 | 2025-12-10 | バイオ - セラピー、フィジオロジカル システムズ フォー ヘルス ソシエタ ペル アチオニ | 治療用又は有益な製品の生物学的効果の発現、持続及び消失を特徴付けるための方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| IL308267A (en) | 2024-01-01 |
| WO2022236011A1 (en) | 2022-11-10 |
| CA3218089A1 (en) | 2022-11-10 |
| AU2022269663A1 (en) | 2023-11-23 |
| KR20240005872A (ko) | 2024-01-12 |
| US20240254545A1 (en) | 2024-08-01 |
| EP4334471A1 (en) | 2024-03-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2024518437A (ja) | 細胞以下の分解能での時空間分解トランスクリプトミクス | |
| Lessel et al. | Germline AGO2 mutations impair RNA interference and human neurological development | |
| EP4295325B1 (en) | Multi-scale spatial transcriptomics analysis | |
| US20230168254A1 (en) | Kits for analysis using nucleic acid encoding and/or label | |
| Bowman et al. | Root of the tree: the significance, evolution, and origins of the ribosome | |
| US11492610B2 (en) | Sample multiplexing for single-cell RNA sequencing | |
| Ushimaru et al. | Biosynthetic origin of the atypical stereochemistry in the thioheptose core of albomycin nucleoside antibiotics | |
| Eklund et al. | Quantification of strand accessibility in biostable DNA origami with single-staple resolution | |
| US11597968B2 (en) | Ribonucleic acid (RNA) interactions | |
| Smoczynski et al. | Advances in the structural and functional understanding of m1A RNA modification | |
| Athar et al. | The human fragile X mental retardation protein inhibits the elongation step of translation through its RGG and C-terminal domains | |
| Qing et al. | Enzymeless DNA base identification by chemical stepping in a nanopore | |
| US20190177785A1 (en) | Nucleic acid modification and identification method | |
| US20200377933A1 (en) | Molecular detection using ligation amplification | |
| US20200216841A1 (en) | Compositions and methods for genomic dna and gene expression analysis in single cells | |
| WO2024159162A1 (en) | Single-molecule peptide sequencing using guanidinylating agents | |
| Kohata et al. | Photoreactive molecular glue for enhancing the efficacy of DNA aptamers by temporary-to-permanent conjugation with target proteins | |
| Onoa et al. | Real-time multistep asymmetrical disassembly of nucleosomes and chromatosomes visualized by high-speed atomic force microscopy | |
| Maqbool et al. | m6A methylation: a new frontier in epilepsy research and therapeutics | |
| Frohlich et al. | Post-transcriptional modifications of RNA: impact on RNA function and human health | |
| Basu et al. | Kineto-mechanistic investigation of effect of macromolecular crowding on the breathing of DNA bubble | |
| CN117616135A (zh) | 亚细胞分辨率的时空分辨转录组学 | |
| WO2022237883A1 (en) | Structural-profiling of analytes by nanopore trapping | |
| Armache et al. | Electron cryo-microscopy structures of remodeler-nucleosome intermediates suggest allosteric control through the nucleosome | |
| Li et al. | Nanopore Identification of N-Acetylation by Hydroxylamine Deacetylation (NINAHD) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20250507 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20250507 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20260422 |