JP2024518358A - 顕微鏡研究用試料の超急速凍結固定方法および装置 - Google Patents
顕微鏡研究用試料の超急速凍結固定方法および装置 Download PDFInfo
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- JP2024518358A JP2024518358A JP2023566941A JP2023566941A JP2024518358A JP 2024518358 A JP2024518358 A JP 2024518358A JP 2023566941 A JP2023566941 A JP 2023566941A JP 2023566941 A JP2023566941 A JP 2023566941A JP 2024518358 A JP2024518358 A JP 2024518358A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Microscoopes, Condenser (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP21170604.9 | 2021-04-27 | ||
| EP21170604.9A EP4083597A1 (en) | 2021-04-27 | 2021-04-27 | Method and device for ultra-rapid cryo-fixation of a sample for microscopic studies |
| EP21212333 | 2021-12-03 | ||
| EP21212333.5 | 2021-12-03 | ||
| PCT/EP2022/061123 WO2022229231A1 (en) | 2021-04-27 | 2022-04-27 | Method and device for ultra-rapid cryo-fixation of a sample for microscopic studies |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2024518358A true JP2024518358A (ja) | 2024-05-01 |
| JP2024518358A5 JP2024518358A5 (https=) | 2025-05-01 |
| JPWO2022229231A5 JPWO2022229231A5 (https=) | 2025-05-01 |
Family
ID=81842049
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023566941A Pending JP2024518358A (ja) | 2021-04-27 | 2022-04-27 | 顕微鏡研究用試料の超急速凍結固定方法および装置 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20240201056A1 (https=) |
| EP (1) | EP4226137B1 (https=) |
| JP (1) | JP2024518358A (https=) |
| WO (1) | WO2022229231A1 (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPWO2024116681A1 (https=) * | 2022-11-29 | 2024-06-06 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013152239A1 (en) | 2012-04-04 | 2013-10-10 | National University Of Singapore | Cryo-preparation systems and methods for near-instantaneous vitrification of biological samples |
| DE102012014791A1 (de) * | 2012-07-25 | 2014-01-30 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Verfahren und Vorrichtung zur Hochgeschwindigkeits-Temperierung einer Probe |
| US20170038105A1 (en) | 2015-08-03 | 2017-02-09 | Michael D. Newman | Pulsed liquid cryogen flow generator |
| EP3418710B1 (en) | 2016-02-19 | 2022-08-24 | Nikon Corporation | Sample preparation method and sample preparation device |
-
2022
- 2022-04-27 US US18/286,563 patent/US20240201056A1/en active Pending
- 2022-04-27 EP EP22725264.0A patent/EP4226137B1/en active Active
- 2022-04-27 WO PCT/EP2022/061123 patent/WO2022229231A1/en not_active Ceased
- 2022-04-27 JP JP2023566941A patent/JP2024518358A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP4226137C0 (en) | 2024-09-25 |
| WO2022229231A1 (en) | 2022-11-03 |
| US20240201056A1 (en) | 2024-06-20 |
| EP4226137B1 (en) | 2024-09-25 |
| EP4226137A1 (en) | 2023-08-16 |
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