JP2024518358A - 顕微鏡研究用試料の超急速凍結固定方法および装置 - Google Patents

顕微鏡研究用試料の超急速凍結固定方法および装置 Download PDF

Info

Publication number
JP2024518358A
JP2024518358A JP2023566941A JP2023566941A JP2024518358A JP 2024518358 A JP2024518358 A JP 2024518358A JP 2023566941 A JP2023566941 A JP 2023566941A JP 2023566941 A JP2023566941 A JP 2023566941A JP 2024518358 A JP2024518358 A JP 2024518358A
Authority
JP
Japan
Prior art keywords
sample
carrier substrate
temperature
pressure
upper side
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2023566941A
Other languages
English (en)
Japanese (ja)
Other versions
JP2024518358A5 (enExample
Inventor
フィリップ イー．ハー． バスティアンス
ヤン ヒュービンガー
エルナン エドガルド グレコ
Original Assignee
マックス プランク ゲゼルシャフト ツゥアー フェデルゥン デル ヴィッセンシャフテン エー フォー
ユニバーシティ オブ ブエノス アイレス
ナショナル サイエンティフィック アンド テクニカル リサーチ カウンシル
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP21170604.9A external-priority patent/EP4083597A1/en
Application filed by マックス プランク ゲゼルシャフト ツゥアー フェデルゥン デル ヴィッセンシャフテン エー フォー, ユニバーシティ オブ ブエノス アイレス, ナショナル サイエンティフィック アンド テクニカル リサーチ カウンシル filed Critical マックス プランク ゲゼルシャフト ツゥアー フェデルゥン デル ヴィッセンシャフテン エー フォー
Publication of JP2024518358A publication Critical patent/JP2024518358A/ja
Publication of JP2024518358A5 publication Critical patent/JP2024518358A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
JP2023566941A 2021-04-27 2022-04-27 顕微鏡研究用試料の超急速凍結固定方法および装置 Pending JP2024518358A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP21170604.9 2021-04-27
EP21170604.9A EP4083597A1 (en) 2021-04-27 2021-04-27 Method and device for ultra-rapid cryo-fixation of a sample for microscopic studies
EP21212333.5 2021-12-03
EP21212333 2021-12-03
PCT/EP2022/061123 WO2022229231A1 (en) 2021-04-27 2022-04-27 Method and device for ultra-rapid cryo-fixation of a sample for microscopic studies

Publications (2)

Publication Number Publication Date
JP2024518358A true JP2024518358A (ja) 2024-05-01
JP2024518358A5 JP2024518358A5 (enExample) 2025-05-01

Family

ID=81842049

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023566941A Pending JP2024518358A (ja) 2021-04-27 2022-04-27 顕微鏡研究用試料の超急速凍結固定方法および装置

Country Status (4)

Country Link
US (1) US12644806B2 (enExample)
EP (1) EP4226137B1 (enExample)
JP (1) JP2024518358A (enExample)
WO (1) WO2022229231A1 (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4628868A4 (en) * 2022-11-29 2026-04-01 The Univ Of Osaka FREEZING DEVICE, MICROSCOPE, FREEZING METHOD AND OBSERVATION METHOD

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59218417A (ja) * 1983-05-25 1984-12-08 Osaka Oxgen Ind Ltd 極低温検鏡法およびそれに用いる顕微鏡
JPH04165320A (ja) * 1990-10-30 1992-06-11 Clarion Co Ltd 光学装置
JP2011508185A (ja) * 2007-11-20 2011-03-10 マックス プランク ゲゼルシャフト ツゥアー フェデルゥン デル ヴィッセンシャフテン エー フォー 超急速冷凍装置及び超急速冷凍方法
WO2013152239A1 (en) * 2012-04-04 2013-10-10 National University Of Singapore Cryo-preparation systems and methods for near-instantaneous vitrification of biological samples
US20140248649A1 (en) * 2013-03-04 2014-09-04 Fei Company Method of Studying a Cryogenic Sample in an Optical Microscope
WO2017141447A1 (ja) * 2016-02-19 2017-08-24 株式会社ニコン 試料作製方法および試料作製装置

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102012014791A1 (de) * 2012-07-25 2014-01-30 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren und Vorrichtung zur Hochgeschwindigkeits-Temperierung einer Probe
US20170038105A1 (en) 2015-08-03 2017-02-09 Michael D. Newman Pulsed liquid cryogen flow generator

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59218417A (ja) * 1983-05-25 1984-12-08 Osaka Oxgen Ind Ltd 極低温検鏡法およびそれに用いる顕微鏡
JPH04165320A (ja) * 1990-10-30 1992-06-11 Clarion Co Ltd 光学装置
JP2011508185A (ja) * 2007-11-20 2011-03-10 マックス プランク ゲゼルシャフト ツゥアー フェデルゥン デル ヴィッセンシャフテン エー フォー 超急速冷凍装置及び超急速冷凍方法
WO2013152239A1 (en) * 2012-04-04 2013-10-10 National University Of Singapore Cryo-preparation systems and methods for near-instantaneous vitrification of biological samples
US20140248649A1 (en) * 2013-03-04 2014-09-04 Fei Company Method of Studying a Cryogenic Sample in an Optical Microscope
WO2017141447A1 (ja) * 2016-02-19 2017-08-24 株式会社ニコン 試料作製方法および試料作製装置

Also Published As

Publication number Publication date
US12644806B2 (en) 2026-06-02
WO2022229231A1 (en) 2022-11-03
US20240201056A1 (en) 2024-06-20
EP4226137A1 (en) 2023-08-16
EP4226137C0 (en) 2024-09-25
EP4226137B1 (en) 2024-09-25

Similar Documents

Publication Publication Date Title
Dahlberg et al. Cryogenic super-resolution fluorescence and electron microscopy correlated at the nanoscale
Schwartz et al. Cryo‐fluorescence microscopy facilitates correlations between light and cryo‐electron microscopy and reduces the rate of photobleaching
Le Gros et al. High‐aperture cryogenic light microscopy
Okolo et al. Sample preparation strategies for efficient correlation of 3D SIM and soft X-ray tomography data at cryogenic temperatures
Godin et al. Super-resolution microscopy approaches for live cell imaging
Fuest et al. In situ microfluidic cryofixation for cryo focused ion beam milling and cryo electron tomography
US20240210291A1 (en) Microscope and observation method
EP4083597A1 (en) Method and device for ultra-rapid cryo-fixation of a sample for microscopic studies
Watanabe et al. Nanometer-resolution fluorescence electron microscopy (nano-EM) in cultured cells
Huebinger et al. Ultrarapid cryo-arrest of living cells on a microscope enables multiscale imaging of out-of-equilibrium molecular patterns
EP4226137B1 (en) Method and device for ultra-rapid cryo-fixation of a sample for microscopic studies
CN113227391A (zh) 激光诱导的细胞转移和分选
Squires et al. Anti-Brownian Traps
Phillips Luminescence lifetimes in biological systems
Gerkau et al. Imaging of local and global sodium signals in astrocytes
Heikal Time-resolved fluorescence anisotropy and fluctuation correlation analysis of major histocompatibility complex class I proteins in fibroblast cells
Spahn et al. Sequential super-resolution imaging of bacterial regulatory proteins, the nucleoid and the cell membrane in single, fixed E. coli cells
EP4628868A1 (en) Freezing device, microscope, freezing method, and observation method
Huebinger et al. Reversible Cryo-arrests of Living Cells to Pause Molecular Movements for High-resolution Imaging
Yamanaka et al. Time-deterministic cryo-optical microscopy
Malfavon et al. Biological Brain Microtubules Interfaced with Semiconductor Qubits
Becker et al. Locating and Visualizing Crystals for X-Ray Diffraction Experiments
Vukojević et al. Fluorescence imaging with single-molecule sensitivity and fluorescence correlation spectroscopy of cell-penetrating neuropeptides
Mlodzianoski et al. Active PSF Shaping and Adaptive Optics Enable Volumetric Single Molecule Super-Resolution Microscopy through Brain Sections
Xu et al. Whole-mount imaging of responses in mouse vomeronasal neurons

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250421

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20250421

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20260325

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20260421

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20260717

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20260728