JP2024506707A - ポリヌクレオチド検出のための方法 - Google Patents

ポリヌクレオチド検出のための方法 Download PDF

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Publication number
JP2024506707A
JP2024506707A JP2023549570A JP2023549570A JP2024506707A JP 2024506707 A JP2024506707 A JP 2024506707A JP 2023549570 A JP2023549570 A JP 2023549570A JP 2023549570 A JP2023549570 A JP 2023549570A JP 2024506707 A JP2024506707 A JP 2024506707A
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JP
Japan
Prior art keywords
oligonucleotide
region
stranded
target
further characterized
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
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JP2023549570A
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English (en)
Japanese (ja)
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JPWO2022175655A5 (https=
JP2024506707A5 (https=
Inventor
ストラレク－ヤヌツキーヴィッチ，マグダレーナ
バームフォース，バーナビー・ウィリアム
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バイオフィデリティ・リミテッド
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Priority claimed from GBGB2102166.2A external-priority patent/GB202102166D0/en
Priority claimed from GBGB2102178.7A external-priority patent/GB202102178D0/en
Application filed by バイオフィデリティ・リミテッド filed Critical バイオフィデリティ・リミテッド
Publication of JP2024506707A publication Critical patent/JP2024506707A/ja
Publication of JPWO2022175655A5 publication Critical patent/JPWO2022175655A5/ja
Publication of JP2024506707A5 publication Critical patent/JP2024506707A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2023549570A 2021-02-16 2022-02-16 ポリヌクレオチド検出のための方法 Pending JP2024506707A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB2102166.2A GB202102166D0 (en) 2021-02-16 2021-02-16 Polynucleotide detection
GB2102166.2 2021-02-16
GBGB2102178.7A GB202102178D0 (en) 2021-02-16 2021-02-16 Polynucleotide detection
GB2102178.7 2021-02-16
PCT/GB2022/050411 WO2022175655A1 (en) 2021-02-16 2022-02-16 Methods for polynucleotide detection

Publications (3)

Publication Number Publication Date
JP2024506707A true JP2024506707A (ja) 2024-02-14
JPWO2022175655A5 JPWO2022175655A5 (https=) 2025-02-07
JP2024506707A5 JP2024506707A5 (https=) 2025-02-07

Family

ID=80462042

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023549570A Pending JP2024506707A (ja) 2021-02-16 2022-02-16 ポリヌクレオチド検出のための方法

Country Status (4)

Country Link
US (1) US20240150818A1 (https=)
EP (1) EP4294944A1 (https=)
JP (1) JP2024506707A (https=)
WO (1) WO2022175655A1 (https=)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010519897A (ja) * 2007-03-01 2010-06-10 オキシテック・リミテッド 核酸検出
JP2010142232A (ja) * 2002-05-10 2010-07-01 City Of Hope 加ピロリン酸分解活性化重合（ｐａｐ）
WO2020016590A1 (en) * 2018-07-19 2020-01-23 Biofidelity Ltd Improved polynucleotide sequence detection method

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6391551B1 (en) 1998-03-13 2002-05-21 Promega Corporation Detection of nucleic acid hybrids
US7033763B2 (en) * 2000-02-23 2006-04-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP)
US11254977B2 (en) 2013-03-12 2022-02-22 Life Technologies Corporation Universal reporter-based genotyping methods and materials

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010142232A (ja) * 2002-05-10 2010-07-01 City Of Hope 加ピロリン酸分解活性化重合（ｐａｐ）
JP2010519897A (ja) * 2007-03-01 2010-06-10 オキシテック・リミテッド 核酸検出
WO2020016590A1 (en) * 2018-07-19 2020-01-23 Biofidelity Ltd Improved polynucleotide sequence detection method

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
QIANG LIU, ET AL.: "Pyrophosphorolysis-Activated Polymerization (PAP): Application to Allele-Specific Amplification", BIOTECHNIQUES, vol. 29, no. 5, JPN6025049287, 29 August 2018 (2018-08-29), pages 1072 - 1083, ISSN: 0005741876 *
R INGRAM, ET AL.: "PAP-LMPCR for improved, allele-specific footprinting and automated chromatin fine structure analysis", NUCLEIC ACIDS RESEARCH, vol. 36, no. 3, JPN6025049289, 21 January 2008 (2008-01-21), pages 1 - 8, ISSN: 0005741877 *

Also Published As

Publication number Publication date
EP4294944A1 (en) 2023-12-27
WO2022175655A1 (en) 2022-08-25
US20240150818A1 (en) 2024-05-09

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