JP2024501524A - キシラナーゼ変異体 - Google Patents

キシラナーゼ変異体 Download PDF

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Publication number
JP2024501524A
JP2024501524A JP2023538842A JP2023538842A JP2024501524A JP 2024501524 A JP2024501524 A JP 2024501524A JP 2023538842 A JP2023538842 A JP 2023538842A JP 2023538842 A JP2023538842 A JP 2023538842A JP 2024501524 A JP2024501524 A JP 2024501524A
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JP
Japan
Prior art keywords
amino acid
xylanase
polypeptide
variant
variant polypeptide
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JP2023538842A
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English (en)
Japanese (ja)
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JP2024501524A5 (https=
Inventor
パロヘイモ、マルヤ
ユントゥネン、カリ
トゥルネン、オッシ
アホラ、ピヒラ
タイパルス、パシ
ミューラー、エウゲン
サヴォライネン、ユッカ
ラングフェルダー、キム
プラネン、テルヒ
Original Assignee
アーベー エンジュメス オサケ ユキチュア
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Application filed by アーベー エンジュメス オサケ ユキチュア filed Critical アーベー エンジュメス オサケ ユキチュア
Publication of JP2024501524A publication Critical patent/JP2024501524A/ja
Publication of JP2024501524A5 publication Critical patent/JP2024501524A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C9/00—After-treatment of cellulose pulp, e.g. of wood pulp, or cotton linters ; Treatment of dilute or dewatered pulp or process improvement taking place after obtaining the raw cellulosic material and not provided for elsewhere
    • D21C9/10—Bleaching ; Apparatus therefor
    • D21C9/1026—Other features in bleaching processes
    • D21C9/1036—Use of compounds accelerating or improving the efficiency of the processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Polymers & Plastics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Medicinal Chemistry (AREA)
  • Birds (AREA)
  • Physiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
JP2023538842A 2020-12-28 2021-12-23 キシラナーゼ変異体 Pending JP2024501524A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20217335.7A EP4019629A1 (en) 2020-12-28 2020-12-28 Xylanase variants
EP20217335.7 2020-12-28
PCT/FI2021/050911 WO2022144500A1 (en) 2020-12-28 2021-12-23 Xylanase variants

Publications (2)

Publication Number Publication Date
JP2024501524A true JP2024501524A (ja) 2024-01-12
JP2024501524A5 JP2024501524A5 (https=) 2025-01-07

Family

ID=73943231

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023538842A Pending JP2024501524A (ja) 2020-12-28 2021-12-23 キシラナーゼ変異体

Country Status (9)

Country Link
US (1) US12600960B2 (https=)
EP (2) EP4019629A1 (https=)
JP (1) JP2024501524A (https=)
KR (1) KR20230125795A (https=)
CN (1) CN117062909A (https=)
CA (1) CA3205919A1 (https=)
CL (1) CL2023001889A1 (https=)
UY (1) UY39589A (https=)
WO (1) WO2022144500A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN120005855B (zh) * 2025-01-20 2026-01-06 江苏科技大学 一种pH稳定性提升的耐高温木聚糖酶突变体及其应用
CN120173917B (zh) * 2025-05-23 2025-08-12 苏州聚维元创生物科技有限公司 一种改性木聚糖酶、dna分子、重组质粒和转染细胞

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003511066A (ja) * 1999-10-12 2003-03-25 カーボザイム・オサケユキテュア ファミリーＧ／１１キシラナーゼの安定性を向上させ、ｐＨ領域を広域化する方法
US7060482B1 (en) * 1998-11-16 2006-06-13 National Research Council Of Canada Thermostable xylanases
WO2011109524A2 (en) * 2010-03-03 2011-09-09 Novozymes, Inc. Xylanase variants and polynucleotides encoding same

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6300114B1 (en) * 1994-07-29 2001-10-09 Rohm Enzyme Finland Oy Sequences of xylanase and xylanase expression vectors
WO2003091402A2 (en) 2002-04-23 2003-11-06 University Of Georgia Research Foundation, Inc. N-ACETYLGLUCOSAMINYLTRANSFERASE Vb CODING SEQUENCE, RECOMBINANT CELLS AND METHODS
EP1668125B1 (en) * 2003-09-15 2014-06-18 Danisco US Inc. Modified enzymes, methods to produce modified enzymes and uses thereof
US7348172B2 (en) * 2004-04-16 2008-03-25 Ab Enzymes Oy Method and DNA constructs for increasing the production level of carbohydrate degrading enzymes in filamentous fungi
ATE469213T1 (de) 2004-04-16 2010-06-15 Ab Enzymes Oy Verfahren und dna-konstrukte zur erhöhung des produktionsniveaus von kohlenhydrat abbauenden enzymen in filamentösen pilzen

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7060482B1 (en) * 1998-11-16 2006-06-13 National Research Council Of Canada Thermostable xylanases
JP2003511066A (ja) * 1999-10-12 2003-03-25 カーボザイム・オサケユキテュア ファミリーＧ／１１キシラナーゼの安定性を向上させ、ｐＨ領域を広域化する方法
WO2011109524A2 (en) * 2010-03-03 2011-09-09 Novozymes, Inc. Xylanase variants and polynucleotides encoding same

Also Published As

Publication number Publication date
US20250019682A1 (en) 2025-01-16
CA3205919A1 (en) 2022-07-07
WO2022144500A1 (en) 2022-07-07
KR20230125795A (ko) 2023-08-29
UY39589A (es) 2022-07-29
CL2023001889A1 (es) 2024-01-05
EP4019629A1 (en) 2022-06-29
CN117062909A (zh) 2023-11-14
EP4267731A1 (en) 2023-11-01
US12600960B2 (en) 2026-04-14

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