JP2024071134A - Antibacterial deodorant comprising microbe and plant component, and antibacterial deodorizing method - Google Patents

Abstract

To provide an antibacterial deodorant that exhibits enhanced abilities through a combination of components extracted from microbes, plants and the like with antibacterial deodorizing capabilities, further extending its effective range to include viruses, and also provide an antibacterial deodorizing method.SOLUTION: An antibacterial deodorant exhibits comprehensive abilities to inhibit the growth of fungi and inactivate virus in space and deodorize the space through a combination of volatile substances released by microbes in a spore state and plants.SELECTED DRAWING: None

Description

The present invention relates to an antibacterial deodorizer and an antibacterial deodorizing method that use spore-forming microorganisms and plant components.

Traditionally, various types of antibacterial and antifungal agents have been developed and used. Some of these use microorganisms for antibacterial and deodorizing purposes. For example, Patent Document 1 describes a deodorizer obtained by impregnating a mineral carrier with various types of microorganisms, such as Bacillus, and then drying them. By spraying this on the sludge to be deodorized, the moisture in the sludge activates the microorganisms, thereby deodorizing the odor.

Antibacterial methods using microorganisms include those that use vaporized substances generated by microorganisms to perform antibacterial and deodorizing functions within a space. For example, Patent Document 2 describes an antibacterial and deodorizing method that uses a novel microorganism belonging to the Bacillus genus.

In addition, components extracted from wood such as cypress are called phytoncides and are known to have antibacterial properties. For example, Patent Document 3 describes a disinfectant spray that uses components extracted from cypress.

JP 07-222791 Patent Publication 2018-007661 JP 4-5210

The microorganisms used in Patent Document 2 utilize "spores," a state that microorganisms form when placed in an environment in which they cannot survive, in order to adapt to that environment. When they reach this state, the microorganisms cease activity and are able to survive dangerous conditions for microorganisms, such as dryness, high temperatures, and low temperatures. When dealing with live microorganisms as commercial products, transportation and storage are particularly problematic, but using the spore state solves this problem and makes them easy to manage.

Patent Document 2 uses microorganisms in the spore state as they are to perform antibacterial and deodorizing, and is an antibacterial method that is safe and effective in every corner of the target space, regardless of the temperature and humidity that are suitable for microbial activity, and can be used not only in room temperature environments such as indoors, but also in a variety of spaces such as refrigerators and air conditioners.

If we could further strengthen the capabilities of this microbial antibacterial deodorant that utilizes the spore state and give it antiviral capabilities, we could provide an even safer and healthier space.

The inventors conducted extensive research into antibacterial deodorants that use microorganisms and have not only antibacterial and deodorizing properties but also antiviral properties. As a result, they discovered that when the microorganisms released by microorganisms are combined with plant components, the virus inactivation ability is significantly increased compared to when each component is used alone, which led to the completion of the present invention. The present invention aims to propose an antibacterial deodorant that has not only antibacterial and deodorizing properties but also antiviral properties.

To achieve the above object, the invention described in claim 1 is an antibacterial deodorant that uses microorganisms and plant components that have deodorizing and antibacterial properties.

The antibacterial deodorant of the present invention is a mixture of a microorganism that has the characteristics of inhibiting the growth of fungi present in a space and deodorizing bad odors, or two or more types of microorganisms including such microorganisms that are supported on a microbial carrier and dried, and a plant component, or a plant component supported on a carrier.

The microorganisms used are safe microorganisms that have deodorizing and antibacterial properties, can form spores, and are not harmful to the human body. For example, the Bacillus genus is one that has all of these characteristics.

A novel gram-positive spore-forming bacillus belonging to the genus Bacillus (a novel microorganism with accession number NITE P-02127, deposited at the National Institute of Technology and Evaluation, Patent Organism Depositary Center and received on October 2, 2015) can be used. This microorganism has the characteristics of inhibiting the growth of fungi and decomposing odorous components.

Here, the term "microorganism carrier" refers to a material capable of retaining microorganisms. Specifically, particulate carriers such as porous glass, ceramics, metal oxides, activated carbon, kaolinite, bentonite, zeolite, silica gel, alumina, anthracite, and perlite can be used; gel carriers such as starch, agar, chitin, chitosan, polyvinyl alcohol, alginic acid, polyacrylamide, carrageenan, agarose, and gelatin; ion-exchange dendritic cellulose, ion-exchange resins, cellulose derivatives, glutaraldehyde, polyacrylic acid, and urethane polymers can be used. Natural or synthetic polymer compounds are also effective, and papers made from cotton, hemp, and pulp materials, which are mainly composed of cellulose, or polymer acetates made by modifying natural materials can also be used. Furthermore, fabrics made from synthetic polymers such as polyester and polyurethane can also be used. These are preferably ones that have good adhesion to microorganisms and have fine gaps. It is also more preferable to use fine materials that can easily penetrate when injected.

Plant components include, for example, trees of the Cupressaceae family, Chamaecyparis obtusifolia, Taiwan cypress, Japanese cypress, Sawara cypress, Lawson cypress, Oriental cypress, Peacock cypress, Crown cypress, Suiryu cypress, Scutellaria cypress, Crown cypress, Crown cypress, and Cypress; trees of the Cupressaceae family, Arborvitae genus, such as Thuja occidentalis and Juniperus japonicus; Japanese cedar, Asarum arborescens; and other trees of the Cupressaceae family, such as Thuja occidentalis and Juniperus japonicus. Trees of the Cupressaceae family, such as Japanese cedar, Japanese cedar, Japanese pencil juniper, and Okinawan juniper; trees of the Cupressaceae family, such as Japanese cedar, Japanese cedar, Japanese cedar, Japanese silverleaf cedar, Japanese red cedar, and Japanese midorisugi; trees of the Cupressaceae family, such as Japanese cedar, Japanese silverleaf cedar, Japanese silverleaf cedar, and Japanese midorisugi; Japanese spruce, fir, Japanese silverleaf ... The essential oils can be extracted from wood, bark, leaves, etc. of one or more kinds selected from the following: Abies veitchii, Abies veitchii, Balsam fir, Mitsumine fir, White fir, Amabilis fir, Abies sachalinensis, California red fir, Grand fir, Noble fir, etc.; Cedrus gracilis, etc.; Picea spp., Picea glabra, etc.; Pinus glabra, Pinus spp., Picea glabra, etc.; Larix larix, etc.; Tsuga hemlock, etc.; Siberian cedar, etc.; Tora pine, etc.; Myrtaceae, etc.; and Eucalyptus, etc.; and the like. Hinokitiol, an antibacterial component extracted from plants of the Cupressaceae family, can also be used.

The carrier for carrying the plant components can be the same as the microbial carrier described above.

The carrier carrying the plant components may be coated to adjust the amount of plant components released. For this coating, natural resins such as rosin and rosin esters, solid waxes such as beeswax and paraffin wax, polymer resins such as polyvinyl alcohol, higher fatty acids, higher alcohols, shellac, sugar esters, etc. can be used.

According to the present invention, the growth of fungi present in a space is suppressed, odors are eliminated, and viruses are inactivated through the synergistic effect of volatile components generated from spore-state microorganisms and plant components, so that the effects can be felt throughout every corner of the space by simply placing the present invention in the space, without the need for tasks such as spraying or applying with a brush. It can also be used indoors in dry conditions or at low temperatures. Moreover, because it is highly safe, it can be used continuously in environments where people live.

The following describes in detail the embodiments of the present invention.

(Example 1)
1-1: Selection of plant components to be used First, the inventors conducted virus inactivation tests using various plant essential oils and microbial powders in order to select the components to be used. As plant essential oils, tea tree essential oil, lavender essential oil, cypress essential oil, eucalyptus essential oil, juniper berry essential oil, marjoram essential oil, ylang-ylang essential oil, frankincense essential oil, palmarosa essential oil, cypress essential oil, and Japanese cedar essential oil were prepared.

1-2: Manufacturing method of microbial powder
Microorganisms belonging to the genus Bacillus and the like form spores when they become in an unsuitable state for survival, such as dry conditions, and become suitable for storage because they are resistant to drying and temperature changes, etc. Taking advantage of this, it is possible to create spore-forming microbial powder by impregnating a porous material with a microbial culture solution and then drying it to promote spore formation.
1 kg of perlite was used as a porous powder carrier, and 2 L of the microbial culture solution of the microorganism to be used was impregnated into it. The carrier carrying the microorganism was then placed under dry conditions at room temperature, and dried to a moisture content of 10% or less to create a spore-forming microorganism powder. Spore-forming microorganism powders were created using microbial strains owned by our company.

1-3: Virus inactivation test in space E. coli phage was used as a substitute for pathogenic viruses in this test. E. coli phage covered by an envelope is said to be less susceptible to antiviral agents than influenza viruses. 20 μl of E. coli phage liquid with 108 class pfu/ml was dropped onto a 1 cm square filter paper and placed inside a 2L sealable box. The sample material was placed there so as not to come into contact with the test bacteria liquid and sealed. If the sample was essential oil, 10 μl was dropped onto a petri dish placed inside the box, and if the sample was microbial powder, 1 g of the powder was placed in a petri dish placed inside the box. After 24 hours, the filter paper was removed and the number of plaques was counted using E. coli as host cells. A blank test without using a sample was also performed in the same way. During these tests, an agar medium made only of water was placed and adjusted so that the humidity inside the container exceeded 90%.

The test results are shown in Table 1. A slight reduction in plaque count was confirmed when plant essential oils such as eucalyptus and cypress were used, and when microbial powder was used, the NITE P-02127 strain was used.

1-4: Preparation of microbial volatile substance replica liquid The microbial powder of the NITE P-02127 strain showed some effectiveness, but a qualitative analysis of the volatile substances emitted was performed and the components were analyzed. A microbial volatile substance replica liquid was created by mixing these components to replicate the volatile substances released from microorganisms. This replica solution contains organic acids and aromatic aldehydes. It also has a higher concentration than the original release concentration. By using this in tests, it is possible to easily distinguish the differences in phenomena that occur when using microorganisms.

1-5: Virus inactivation test in the air Using the aforementioned essential oils and NITE P-02127 strain microbial volatile substance replica liquids that showed some degree of effectiveness, these were mixed and the aforementioned virus inactivation test in the air was performed again. If the specimen was a liquid, 10 μl was dropped into a petri dish placed in the box, and if the specimen was a liquid and a powder, 5 μl of the liquid and 0.5 g of the powder were placed in each of the two petri dishes placed in the box.

The test results are shown in Table 2. When each essential oil was mixed with the liquid that reproduced microbial volatile substances, an improvement in performance was observed in greatly reducing the number of plaques. The ability was also enhanced when Hiba and Hinoki were mixed. In addition, a large improvement in ability was observed when the microbial powder and the Hiba and Hinoki essential oil mixture were used simultaneously.

It was found that when wood components such as hiba and hinoki essential oils and volatile substances from microbial powder of a microorganism (NITE P-02127 strain) are used simultaneously, a synergistic effect occurs, demonstrating high virus inactivation capabilities.

Some of the components contained in the microbial powder replica liquid are not suitable for long-term release due to degradation in the air, but spore-forming microorganisms continue to produce these components over a long period of time, so if used in combination with a formulation that releases plant components, it is possible to inactivate viruses in the space for a long period of time.

Example 2
2-1: Manufacturing method of microbial powder 1 kg of perlite as a porous powder carrier was impregnated with 2 L of microbial culture solution of the microorganism to be used. The carrier carrying the microorganism was then placed under dry conditions at room temperature and dried to a moisture content of 10% or less to produce spore-forming microbial powder. The above-mentioned NITE P-02127 strain was used as the microorganism to produce spore-forming microbial powder 1.

2-2: Method for producing phytoncide beads having sustained release properties Next, phytoncide beads can be produced by embedding a mixture of plant components and a coating material such as rosin into the pores of the carrier.
Viscopearl (manufactured by Rengo Co., Ltd.) was used as a carrier. 0.3 kg of these cellulose beads were mixed with 1 kg of heat-melted rosin ester AA-G (manufactured by Arakawa Chemical Industries Co., Ltd.) and 0.1 kg of plant components consisting of hiba essential oil, hinoki cypress essential oil, etc., and the mixture was allowed to penetrate and stir to create phytoncide beads 2.
The phytoncide beads 2 and the microbial powder 1 were mixed in equal amounts to form a microbial phyton agent 3.

2-2: Test method for virus inactivation duration test in space The test bacteria used in this test were E. coli phages as a substitute for pathogenic viruses. 20 μl of E. coli phage liquid with 108 class pfu/g was dropped onto a 1 cm square filter paper and placed inside a 2 L sealable box. 2 g of microbial phyton agent 3 was placed there as a specimen so as not to come into contact with the test bacteria liquid and sealed. After 24 hours, the filter paper was removed and the number of plaques was counted using E. coli as host cells. A blank test without using a specimen was also performed in the same way. During these tests, water was dropped into the container as appropriate to adjust the humidity inside the container to exceed 90%.
After the test was completed, the microbial phyton agent 3 was removed and left in an open room, and after a certain number of days had passed, the same test was repeated again.

The test results are shown in Table 3. We were able to confirm the effect of significantly reducing the number of phages even after a considerable number of days had passed. This is thought to be due to the sustained release properties of the coated beads and the long-term effectiveness of the microbial powder in spore form, both of which continued to be effective for a long period of time.

2-3: Virus inactivation test in the air A virus inactivation test similar to that described above was conducted using influenza virus instead of E. coli phage. A 2 cm circular filter (made of glass) was placed and the influenza virus (Influenza virus H1N1 A/PR/8/34 ATCC VR-1469) was inoculated into the air.
) 0.05 mL of 7x10^7 PFU/mL was dropped into each well and placed inside a 2L sealable box. 2 g of Microbial Phyton Agent 3 was placed there as a specimen, and after 6 hours, the filter paper was removed and the number of plaques was counted using MDCK cells (canine kidney cells) as host cells, and the viral infection titer was measured.

The test results are shown in Table 4. A high inactivation capacity of 99.99% was confirmed not only for phages but also for viruses.

2-4: Deodorizing and antibacterial tests The microbial powder 1 that constitutes the microbial phyton agent 3 has antibacterial properties against fungi and deodorizing properties against ammonia, amines, etc. Also, as is generally known, Japanese cypress and hinoki cypress have antibacterial and deodorizing properties, so this microbial phyton agent 3 has the above-mentioned antiviral effect as well as antibacterial and deodorizing properties.

Deodorization test: A 10L Baron Box was prepared and the odor to be tested was generated inside at room temperature. 1 g of Microbial Phyton Agent 3 was placed in a nonwoven bag, sealed, and hung in the Baron Box so as not to touch the wall, and the concentration of the test substance was observed. The detector tube method from Gastec Corporation was used for the observation.

Table 5 shows the changes in analyte concentrations. A decrease in the analytes ammonia, trimethylamine, and hydrogen sulfide was confirmed.

Antibacterial test: A normal agar medium coated with Escherichia coli and Staphylococcus aureus was placed inside a 2L sealable box. Microbial Phyton Agent 3 was placed there without contact and the box was sealed, left to stand at room temperature for 48 hours, and growth on the medium was observed. A control was also prepared in which no microbial Phyton Agent was placed.

In the control, Escherichia coli and Staphylococcus aureus formed large numbers of colonies on the agar medium, but in the area where Microbial Phyton Agent 3 was placed, no colonies could be seen with the naked eye. It is believed that the proliferation of these microorganisms was suppressed.

According to the present invention, the antibacterial, deodorant, and antiviral agent and method, which utilize microorganisms and plants that exist in nature and have excellent safety, can be used in various spaces. In the home, it can be used in rooms, air conditioners, entrances, toilets, etc.

Claims (5)

An antibacterial deodorizer that combines the volatile substances released by spore-state microorganisms with plant ingredients to inhibit fungal growth in the air, inactivate viruses, and eliminate odors. The antibacterial deodorant according to claim 1, wherein the microorganism used is a microorganism having the accession number NITE P-02127. The antibacterial deodorant according to claim 1, wherein the plant component used contains essential oils from plants of the Cupressaceae family or Eucalyptus genus. The antibacterial deodorant according to claim 1, wherein the plant ingredients used include essential oils of cypress and Japanese cypress. An antibacterial method using the antibacterial deodorant according to any one of claims 1 to 4.