JP2023527504A - Monoclonal antibody 20D8 against SARS-CoV-2 epidemic variant - Google Patents
Monoclonal antibody 20D8 against SARS-CoV-2 epidemic variant Download PDFInfo
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Abstract
本発明は、野生型ＳＡＲＳＣｏＶ－２と、Ｐ．１株、Ｂ．１．３５１株、Ｂ．１．１．７株を含む複数のＳＡＲＳ－ＣｏＶ－２変異株を中和できる、ＳＡＲＳ－ＣｏＶ－２流行性変異株に対するモノクローナル抗体２０Ｄ８に関する。【選択図】図４The present invention provides wild-type SARSCoV-2 and P. 1 strain, B.I. 1.351 strain, B. 1. to monoclonal antibody 20D8 against the SARS-CoV-2 epidemic variant, which can neutralize multiple SARS-CoV-2 variants including strain 1.7. [Selection drawing] Fig. 4
Description
本発明は、バイオ医薬品技術の分野に属し、詳しく言えば、ＳＡＲＳ－ＣｏＶ－２流行
性変異株に対するモノクローナル抗体２０Ｄ８に関する。
The present invention belongs to the field of biopharmaceutical technology and in particular relates to the monoclonal antibody 20D8 against the SARS-CoV-2 epidemic variant.
ＳＡＲＳ－ＣｏＶ－２は、感染率と致死率がどちらも高い新型のコロナウイルスで、そ
の感染が発生して以来、既に世界的な大流行を引き起こしており、世界的に深刻な公共健
康問題が起きている。ワクチンは感染症を効果的に予防でき、現在、一部の新型コロナワ
クチンが使用承認されているが、主に健康な集団を対象としている。ＳＡＲＳ－ＣｏＶ－
２に対する中和抗体はウイルスの細胞との結合を効果的にブロックすることができ、臨床
試験では良好な結果が得られている。
SARS-CoV-2, a novel coronavirus with both high infection and fatality rates, has already caused a global pandemic since its outbreak and has become a serious public health problem worldwide. stay up. Vaccines can effectively prevent infectious diseases, and some Covid-19 vaccines are currently approved for use, but mainly for healthy populations. SARS-CoV-
2 can effectively block virus binding to cells, with good results in clinical trials.
現在、一部のＳＡＲＳ－ＣｏＶ－２に対するモノクローナル抗体は緊急使用が承認され
ており、開発中のものも多い。しかし、既存の抗体の殆どは主に変異前のＳＡＲＳ－Ｃｏ
Ｖ－２野生株（Ｗｉｌｄ－ｔｙｐｅ、ＷＴ）を対象としている。Ｐ．１株（Ｋ４１７Ｔ、
Ｅ４８４Ｋ、Ｎ５０１Ｙなどの変異を含む）、Ｂ．１．３５１（５０１Ｙ．Ｖ２とも呼ば
れ、Ｋ４１７Ｎ、Ｅ４８４Ｋ、Ｎ５０１Ｙなどの変異を含む）、Ｂ．１．１．７（Ｅ４８
４Ｋ、Ｎ５０１Ｙなどの変異を含む）などの新たに出現した変異株は、スパイクタンパク
質（ＲＢＤ及びＮＴＤ領域を含む）アミノ酸配列における複数の点変異が変異株の伝播速
度、病原性に影響を与えるだけでなく、モノクローナル抗体の中和活性にも大きな影響を
与えるため、ウイルスの免疫回避が起こり得る。そのために、ＳＡＲＳ－ＣｏＶ－２野生
株及び変異株に広域な中和能を有するモノクローナル抗体の開発が急務となり、ＳＡＲＳ
－ＣｏＶ－２の野生株及び変異株などの検出と治療におけるその大幅な使用が見込まれる
。
Currently, some monoclonal antibodies against SARS-CoV-2 are approved for emergency use, and many more are under development. However, most of the existing antibodies are mainly SARS-Co
V-2 wild-type (WT) is targeted. P. 1 strain (K417T,
E484K, including mutations such as N501Y), B. 1.351 (also called 501Y.V2, containing mutations such as K417N, E484K, N501Y); 1.1.7 (E48
4K, N501Y, etc.), only multiple point mutations in the amino acid sequence of the spike protein (including the RBD and NTD regions) affect the transmission rate and virulence of the mutant. However, it also greatly affects the neutralizing activity of monoclonal antibodies, which can lead to immune evasion of the virus. Therefore, there is an urgent need to develop monoclonal antibodies that have broad-spectrum neutralization ability against SARS-CoV-2 wild type and mutant strains.
- Potential for its significant use in the detection and treatment of CoV-2 wild-type and mutant strains, etc.
本発明は、６つのＣＤＲ領域が、
（１）配列番号１のアミノ酸配列ＥＹＴＭＹを含む重鎖ＣＤＲ１（ＶＨＣＤＲ１）と、
（２）配列番号２のアミノ酸配列ＧＩＮＰＮＩＧＤＴＧＹＮＱＫＦＫＧを含む重鎖ＣＤ
Ｒ２（ＶＨＣＤＲ２）と、
（３）配列番号３のアミノ酸配列ＤＴＧＮＹＰＦＤＹを含む重鎖ＣＤＲ３（ＶＨＣＤＲ
３）と、
（４）配列番号４のアミノ酸配列ＫＳＳＱＳＬＬＹＳＳＮＱＫＮＹＬＡを含む軽鎖ＣＤ
Ｒ１（ＶＬＣＤＲ１）と、
（５）配列番号５のアミノ酸配列ＷＡＳＴＲＥＳを含む軽鎖ＣＤＲ２（ＶＬＣＤＲ２）
と、
（６）配列番号６のアミノ酸配列ＱＱＹＹＳＹＰＬＴを含む軽鎖ＣＤＲ３（ＶＬＣＤＲ
３）とであることを特徴とするＳＡＲＳ－ＣｏＶ－２ウイルスに対するモノクローナル抗
体２０Ｄ８に関する。
The present invention provides that the six CDR regions are
(1) a heavy chain CDR1 (VHCDR1) comprising the amino acid sequence EYTMY of SEQ ID NO: 1;
(2) a heavy chain CD comprising the amino acid sequence GINPNIGDTGYNQKFKG of SEQ ID NO:2
R2 (VHCDR2);
(3) heavy chain CDR3 comprising the amino acid sequence DTGNYPFDY of SEQ ID NO: 3 (VHCDR
3) and
(4) a light chain CD comprising the amino acid sequence KSSQSLLYSSNQKNYLA of SEQ ID NO:4
R1 (VLCDR1);
(5) a light chain CDR2 (VLCDR2) comprising the amino acid sequence WASTRES of SEQ ID NO:5
and,
(6) a light chain CDR3 comprising the amino acid sequence QQYYSYPLT of SEQ ID NO: 6 (VLCDR
3) to the monoclonal antibody 20D8 against the SARS-CoV-2 virus characterized by:
さらに、前記モノクローナル抗体２０Ｄ８の重鎖可変領域の全長は配列番号７のアミノ
酸配列ＥＶＱＬＱＱＳＧＰＥＬＶＫＰＧＡＳＶＫＩＳＣＫＴＳＧＹＴＦＴＥＹＴＭＹＷＶ
ＫＱＳＨＧＫＳＬＥＷＩＧＧＩＮＰＮＩＧＤＴＧＹＮＱＫＦＫＧＫＡＴＬＴＶＤＫＳＳＳ
ＴＡＹＭＥＩＲＳＬＴＳＥＤＳＡＶＹＹＣＡＲＤＴＧＮＹＰＦＤＹＷＧＱＧＴＴＬＴＶＳ
Ｓを含み、
前記モノクローナル抗体２０Ｄ８の軽鎖可変領域の全長は配列番号８のアミノ酸配列Ｄ
ＩＶＭＳＱＳＰＳＳＬＡＶＳＶＧＥＫＶＴＭＳＣＫＳＳＱＳＬＬＹＳＳＮＱＫＮＹＬＡＷ
ＹＱＱＫＰＧＱＳＰＫＶＬＩＹＷＡＳＴＲＥＳＧＶＰＤＲＦＴＧＳＧＳＧＴＤＦＴＬＴＩ
ＳＳＶＫＡＥＤＬＡＶＹＹＣＱＱＹＹＳＹＰＬＴＦＧＡＧＴＫＬＥＬＫを含む。
Furthermore, the full-length heavy chain variable region of the monoclonal antibody 20D8 has the amino acid sequence EVQLQQSGPELVKPGASVKISCKTSGYTFTEYTMYWV of SEQ ID NO:7.
KQSHGKSLEWIGGINPNIGDTGYNQKFKGKATLTVDKSSS
TAYMEIRSLTSEDSAVYYCARDTGNYPFDYWGQGTTLTVS
including S,
The full length of the light chain variable region of the monoclonal antibody 20D8 is the amino acid sequence D of SEQ ID NO:8
IVMSQSPSSLAVSVGEKVTMSCKSSQSLLYSSNQKNYLAW
YQQKPGQSPKVLIYWASTRESGVPDRFTGSGSGTDFTLTI
SSVKAEDLAVYYCQQYYSYPLTFGAGTKLELK.
さらに、前記モノクローナル抗体２０Ｄ８はＩｇＧ型抗体である。 Furthermore, said monoclonal antibody 20D8 is an IgG type antibody.
さらに、前記モノクローナル抗体２０Ｄ８は、野生型ＳＡＲＳ－ＣｏＶ－２と、Ｐ．１
株、Ｂ．１．３５１株、Ｂ．１．１．７株を含む複数のＳＡＲＳ－ＣｏＶ－２変異株を中
和することができる。
In addition, the monoclonal antibody 20D8 is a wild-type SARS-CoV-2 and P. 1
strain, B. 1.351 strain, B. It is able to neutralize multiple SARS-CoV-2 mutant strains including the 1.1.7 strain.
具体的には、前記モノクローナル抗体２０Ｄ８が結合する抗原構造領域はＳＡＲＳ－Ｃ
ｏＶ－２ウイルスのスパイクタンパク質Ｓ１のＲＢＤドメインであり、且つ、Ｄ６１４Ｇ
、Ｋ４１７Ｔ／Ｎ、Ｅ４８４Ｋ、Ｎ５０１Ｙなどの変異を含むＳＡＲＳ－ＣｏＶ－２のス
パイクタンパク質Ｓ１に結合することができる。
Specifically, the antigen structure region to which the monoclonal antibody 20D8 binds is SARS-C
oV-2 virus spike protein S1 RBD domain, and D614G
, K417T/N, E484K, N501Y and other mutations to SARS-CoV-2 spike protein S1.
本発明は、さらに、前記モノクローナル抗体２０Ｄ８をコードする核酸フラグメントに
関する。
The invention further relates to a nucleic acid fragment encoding said monoclonal antibody 20D8.
本発明は、さらに、
軽鎖が、
（１）配列番号８のアミノ酸配列に１つ又は複数のアミノ酸が置換、欠失又は付加され
て形成された同じ機能を有する配列であり、
又は、（２）配列番号８のアミノ酸配列と９５％以上の相同性を有するアミノ酸配列で
あり、
重鎖が、
（１）配列番号７のアミノ酸配列に１つ又は複数のアミノ酸が置換、欠失又は付加され
て形成された同じ機能を有する配列であり、
又は、（２）配列番号７のアミノ酸配列と９５％以上の相同性を有するアミノ酸配列で
ある、抗体に関する。
The present invention further provides
the light chain
(1) a sequence having the same function formed by substituting, deleting or adding one or more amino acids to the amino acid sequence of SEQ ID NO:8;
or (2) an amino acid sequence having 95% or more homology with the amino acid sequence of SEQ ID NO: 8,
the heavy chain
(1) a sequence having the same function formed by substituting, deleting or adding one or more amino acids to the amino acid sequence of SEQ ID NO: 7;
or (2) an antibody having an amino acid sequence having 95% or more homology with the amino acid sequence of SEQ ID NO:7.
本発明は、さらに、ＳＡＲＳ－ＣｏＶ－２ウイルスを検出する試薬の製造における前記
モノクローナル抗体２０Ｄ８の使用を含み、好ましくは、前記試薬はＳＡＲＳ－ＣｏＶ－
２ウイルスＰ．１株、Ｂ．１．３５１株、Ｂ．１．１．７株を検出する試薬である。
The present invention further includes the use of said monoclonal antibody 20D8 in the manufacture of a reagent for detecting SARS-CoV-2 virus, preferably said reagent is SARS-CoV-
2 virus P. 1 strain, B.I. 1.351 strain, B. 1.1.7 strain detection reagent.
本発明は、さらに、ＳＡＲＳ－ＣｏＶ－２ウイルスを阻害する試薬の製造における前記
モノクローナル抗体２０Ｄ８の使用を含み、好ましくは、前記試薬はＳＡＲＳ－ＣｏＶ－
２ウイルスＰ．１株、Ｂ．１．３５１株、Ｂ．１．１．７株を阻害する試薬である。
The present invention further includes the use of said monoclonal antibody 20D8 in the manufacture of a reagent that inhibits the SARS-CoV-2 virus, preferably said reagent is SARS-CoV-
2 virus P. 1 strain, B.I. 1.351 strain, B. 1.1.7 strain inhibition reagent.
本発明は、さらに、医薬品の製造におけるモノクローナル抗体２０Ｄ８の使用を含み、
前記医薬品はＳＡＲＳ－ＣｏＶ－２ウイルスへの感染に起因する疾患を予防及び／又は
治療する医薬品であり、好ましくは、前記医薬品はＳＡＲＳ－ＣｏＶ－２ウイルスＰ．１
株、Ｂ．１．３５１株、Ｂ．１．１．７株への感染に起因する疾患を予防及び／又は治療
する医薬品である。
The invention further includes the use of monoclonal antibody 20D8 in the manufacture of a medicament,
Said medicament is a medicament for preventing and/or treating a disease caused by infection with SARS-CoV-2 virus, preferably said medicament is SARS-CoV-2 virus P. 1
strain, B. 1.351 strain, B. A medicament for preventing and/or treating diseases caused by infection with the 1.1.7 strain.
本発明の有益な効果は次のとおりである。現在開発されているワクチン及び緊急使用が
承認された一部の抗体は、主に変異前のＳＡＲＳ－ＣｏＶ－２野生株を対象としている。
Ｐ．１株、Ｂ．１．３５１株、Ｂ．１．１．７株などのＳＡＲＳ－ＣｏＶ－２変異株は、
程度の差があるが、開発中の一部の抗体や、回復期患者の血漿、ワクチン免疫化後の血漿
に耐性を示している。本発明の２０Ｄ８モノクローナル抗体に関する実験結果から、当該
抗体２０Ｄ８はＳＡＲＳ－ＣｏＶ－２野生株と、Ｐ．１株、Ｂ．１．３５１株、Ｂ．１．
１．７株などの変異株に強い中和活性を有することが示されている。そのため、本発明か
ら得られる、広域な中和活性を有するモノクローナル抗体２０Ｄ８はＳＡＲＳ－ＣｏＶ－
２野生株及びその変異株に起因する疾患の特異的な予防、治療及び診断のための研究と使
用に大きな価値があるだろう。
Beneficial effects of the present invention are as follows. Vaccines currently in development and some antibodies approved for emergency use are primarily directed against the pre-mutated SARS-CoV-2 wild strain.
P. 1 strain, B.I. 1.351 strain, B. SARS-CoV-2 mutants, such as strain 1.1.7,
To varying degrees, it is resistant to some antibodies under development, plasma from convalescent patients, and plasma after vaccination. From the experimental results on the 20D8 monoclonal antibody of the present invention, the antibody 20D8 is the SARS-CoV-2 wild strain and P. 1 strain, B.I. 1.351 strain, B. 1.
It has been shown to have strong neutralizing activity against mutant strains such as 1.7 strain. Therefore, the monoclonal antibody 20D8 obtained from the present invention, which has broad-spectrum neutralizing activity, is SARS-CoV-
It would be of great value for research and use for the specific prevention, treatment and diagnosis of diseases caused by the 2 wild strain and its mutant strains.
特段の説明がない限り、下記の実施例で用いられる技術的手段はいずれも当業者に熟知
される通常の手段で、全ての試薬が市販品である。
Unless otherwise specified, all technical means used in the following examples are conventional means well known to those skilled in the art, and all reagents are commercially available products.
実施例１：モノクローナル抗体２０Ｄ８の製造及び精製
１．製造：
７日前にフロイント不完全アジュバントを注射した後、２０Ｄ８抗体を安定的に分泌す
るモノクローナルハイブリドーマ細胞株（０．５ｍＬ、３×１０６細胞／ｍＬ）をマウス
の腹腔に注射して、引き続き７～１０日間培養した。
Example 1: Production and Purification of Monoclonal Antibody 20D81. Manufacturing:
After injection with incomplete Freund's adjuvant 7 days earlier, mice were intraperitoneally injected with a monoclonal hybridoma cell line (0.5 mL, 3×10 6 cells/mL) stably secreting 20D8 antibody and subsequently injected 7-10 days later. cultured for days.
２．精製：
腹水を採取し、３７℃下で２時間静置した後、５０００ｒｐｍで３０分間遠心分離し、
中間部の上清を回収して濾過し、続いてプロテインＧアフィニティークロマトグラフィー
により精製した。精製ステップを要約すると次のとおりであった。
ｐＨが７．０で０．１ＭのＴｒｉｓバッファーで平衡化させた。
サンプルを注入した後に、ｐＨが７．０で０．１ＭのＴｒｉｓバッファーで溶出した。
続いて、ｐＨが８．０で１．０ＭのＴｒｉｓバッファーで溶出した。
2. purification:
Ascites was collected, allowed to stand at 37°C for 2 hours, centrifuged at 5000 rpm for 30 minutes,
Intermediate supernatants were collected, filtered and subsequently purified by protein G affinity chromatography. A summary of the purification steps was as follows.
Equilibrated with 0.1 M Tris buffer at pH 7.0.
After injecting the sample, it was eluted with 0.1 M Tris buffer at pH 7.0.
Subsequently, it was eluted with 1.0 M Tris buffer at pH 8.0.
溶出液を回収してＰＢＳバッファーにおいて透析した。精製後の抗体に対し、ＳＤＳ－
ＰＡＧＥ及びＳＥＣ－ＨＰＬＣによる検出と解析を行った。
The eluate was collected and dialyzed in PBS buffer. SDS-
Detection and analysis were performed by PAGE and SEC-HPLC.
３．結果分析：
ＳＤＳ－ＰＡＧＥ結果である図１に示されるとおり、非還元条件では、２０Ｄ８抗体が
、分子量が約１５０ｋＤａのバンドとして現われた。還元条件では、分子量が約５０ｋＤ
ａと２５ｋＤａの２つのバンドとして現われ、それぞれが抗体の重鎖及び軽鎖に対応する
。ＳＥＣ－ＨＰＬＣ結果である図２からは、精製後のモノクローナル抗体の純度が９９％
以上に達していることが示されていた。精製後のモノクローナル抗体に対しペプチドマッ
プ法で解析したアミノ酸配列が想定したアミノ酸配列に一致した。
3. Results analysis:
As shown in Figure 1, which is the SDS-PAGE result, under non-reducing conditions, the 20D8 antibody appeared as a band with a molecular weight of approximately 150 kDa. Under reducing conditions, the molecular weight is about 50 kD
It appears as two bands of a and 25 kDa, corresponding respectively to the heavy and light chains of the antibody. From FIG. 2, which is the SEC-HPLC result, the purity of the monoclonal antibody after purification is 99%.
It was shown that more than The amino acid sequence of the purified monoclonal antibody analyzed by the peptide map method matched the expected amino acid sequence.
具体的には、前記２０Ｄ８抗体の６つのＣＤＲ領域の配列構造は、
配列番号１のＥＹＴＭＹである重鎖ＣＤＲ１（ＶＨＣＤＲ１）と、
配列番号２のＧＩＮＰＮＩＧＤＴＧＹＮＱＫＦＫＧである重鎖ＣＤＲ２（ＶＨＣＤＲ２
）と、
配列番号３のＤＴＧＮＹＰＦＤＹである重鎖ＣＤＲ３（ＶＨＣＤＲ３）と、
配列番号４のＫＳＳＱＳＬＬＹＳＳＮＱＫＮＹＬＡである軽鎖ＣＤＲ１（ＶＬＣＤＲ１
）と、
配列番号５のＷＡＳＴＲＥＳである軽鎖ＣＤＲ２（ＶＬＣＤＲ２）と、
配列番号６のＱＱＹＹＳＹＰＬＴである軽鎖ＣＤＲ３（ＶＬＣＤＲ３）とである。
Specifically, the sequence structure of the six CDR regions of the 20D8 antibody is
a heavy chain CDR1 (VHCDR1) that is EYTMY of SEQ ID NO: 1;
Heavy chain CDR2 (VHCDR2
)and,
a heavy chain CDR3 (VHCDR3) that is DTGNYPFDY of SEQ ID NO: 3;
light chain CDR1 (VLCDR1
)and,
a light chain CDR2 (VLCDR2) that is the WASTRES of SEQ ID NO: 5;
and the light chain CDR3 (VLCDR3), which is QQYYSYPLT of SEQ ID NO:6.
前記２０Ｄ８抗体の軽鎖可変領域及び重鎖可変領域の配列は、それぞれ、配列番号８、
配列番号７である。
配列番号７：ＥＶＱＬＱＱＳＧＰＥＬＶＫＰＧＡＳＶＫＩＳＣＫＴＳＧＹＴＦＴＥＹＴ
ＭＹＷＶＫＱＳＨＧＫＳＬＥＷＩＧＧＩＮＰＮＩＧＤＴＧＹＮＱＫＦＫＧＫＡＴＬＴＶＤ
ＫＳＳＳＴＡＹＭＥＩＲＳＬＴＳＥＤＳＡＶＹＹＣＡＲＤＴＧＮＹＰＦＤＹＷＧＱＧＴＴ
ＬＴＶＳＳ。
配列番号８：ＤＩＶＭＳＱＳＰＳＳＬＡＶＳＶＧＥＫＶＴＭＳＣＫＳＳＱＳＬＬＹＳＳ
ＮＱＫＮＹＬＡＷＹＱＱＫＰＧＱＳＰＫＶＬＩＹＷＡＳＴＲＥＳＧＶＰＤＲＦＴＧＳＧＳ
ＧＴＤＦＴＬＴＩＳＳＶＫＡＥＤＬＡＶＹＹＣＱＱＹＹＳＹＰＬＴＦＧＡＧＴＫＬＥＬＫ
。
The sequences of the light chain variable region and heavy chain variable region of the 20D8 antibody are, respectively, SEQ ID NO: 8,
SEQ ID NO:7.
SEQ ID NO: 7: EVQLQQSGPELVKPGASVKISCCKTSGYTFTEYT
MYWVKQSHGKSLEWIGGINPNIGDT GYNQKFKGKATLTVD
KSSSTAYMEIRSLTSEDSAVYYCARDTGNYPFDYWGQGTT
LTVSS.
SEQ ID NO: 8: DIVMSQSPSSLAVSVGEKVTMSCKSSQSLLYSS
NQKNYLAWYQQKPGQSPKVLIYWASTRESGVPDRFTGSGS
GTDFTLTISSVKAEDLAVYYCQQYYSYPLTFGAGTKLELK
.
実施例２：抗体の機能分析
１． ＳＡＲＳ－ＣｏＶ－２に対するモノクローナル抗体２０Ｄ８の抗原結合活性の検
出
ＥＬＩＳＡ法で２０Ｄ８抗体のＳＡＲＳ－ＣｏＶ－２ウイルスのスパイクタンパク質Ｓ
１に対する結合能を測定した。ステップを要約すると次のとおりであった。
（１）ＳＡＲＳ－ＣｏＶ－２スパイクＳ１－Ｈｉｓ組換えタンパク質（Ｓｉｎｏ社、カ
タログ番号：４０５９１－Ｖ０８Ｈ）をコーティング抗原として、マイクロプレートに炭
酸塩バッファーを使用して２．０μｇ／ｍＬの抗原をコーティングし、３７℃下で２時間
インキュベートした。
（２）カゼインバッファーを使用して３７℃下で２時間ブロックし、段階的に希釈した
被検抗体を加えて、３７℃下で１時間インキュベートした。
（３）１：１００００に希釈したヤギ抗マウスＩｇＧ－ＨＲＰ（Ｂｉｏｄｒａｇｏｎ社
、カタログ番号：ＢＦ０３００１Ｘ）を加えて、３７℃下で１時間インキュベートした。
（４）発色溶液で発色させて、２Ｍ ＨＣｌで反応を停止させた。マイクロプレートリ
ーダーにおいて４５０ｎｍの吸光度を検出した。
Example 2: Antibody
The binding capacity to 1 was measured. The steps were summarized as follows.
(1) SARS-CoV-2 spike S1-His recombinant protein (Sino, catalog number: 40591-V08H) as a coating antigen, and 2.0 μg/mL of antigen is coated on a microplate using carbonate buffer. and incubated at 37°C for 2 hours.
(2) Block with casein buffer at 37°C for 2 hours, add serially diluted test antibodies, and incubate at 37°C for 1 hour.
(3) Goat anti-mouse IgG-HRP (Biodragon, catalog number: BF03001X) diluted to 1:10000 was added and incubated at 37° C. for 1 hour.
(4) Developed with developing solution and quenched with 2M HCl. Absorbance at 450 nm was detected in a microplate reader.
結果：
２０Ｄ８抗体の抗原に対する結合活性の検出結果である図３に示されるように、モノク
ローナル抗体２０Ｄ８のＳ１タンパク質に対する結合活性がＥＣ５０＝０．０５０ｎＭで
あった。
result:
As shown in FIG. 3, which is the detection result of the antigen-binding activity of the 20D8 antibody, the binding activity of the monoclonal antibody 20D8 to the S1 protein was EC 50 =0.050 nM.
２． ＳＡＲＳ－ＣｏＶ－２に対するモノクローナル抗体２０Ｄ８の（変異型）シュー
ドウイルスに対する中和試験
シュードウイルス検出系を使用して２０Ｄ８のＳＡＲＳ－ＣｏＶ－２野生株（ＷＴ）及
びその変異株に対する中和活性として（シュードウイルスは全て北京天薬物生物技術開発
会社から購入した）、野生株（カタログ番号：８００３３）、Ｐ．１株（カタログ番号：
８００４５）、Ｂ．１．３５１株（カタログ番号：８００４４）及びＢ．１．１．７株（
カタログ番号：８００４３）に対応するシュードウイルスへの中和活性を検出した。ステ
ップを要約すると次のとおりであった。段階的に希釈した抗体２０Ｄ８とシュードウイル
スを混合させて３７℃下で１時間インキュベートし、対照群は抗体又はウイルスを加えな
かった。その後、予め用意したＨｕｈ７単層細胞を含む検出プレートに加えて２４時間培
養し、続いて、１００μＬの培養上清を等量の蛍光基質と交換して、室温下で２分間イン
キュベートして１５０μＬのライセートを新しい９６ウェルプレートに移して蛍光値を測
定し、シュードウイルスの中和活性を計算した（ＩＣ５０で示した）。
2. Neutralization test of monoclonal antibody 20D8 against SARS-CoV-2 (mutant) pseudovirus As a neutralizing activity of 20D8 against SARS-CoV-2 wild type (WT) and its mutants using a pseudovirus detection system ( All pseudoviruses were purchased from Beijing Tian Pharmaceutical Biotechnology Development Company), wild strain (catalogue number: 80033), P. 1 strain (catalog number:
80045), B. 1.351 strain (catalog number: 80044) and B. 1.1.7 shares (
A pseudovirus-neutralizing activity corresponding to (catalog number: 80043) was detected. The steps were summarized as follows. The serially diluted antibody 20D8 and the pseudovirus were mixed and incubated at 37°C for 1 hour, and the control group received no antibody or virus. It was then added to a detection plate containing Huh7 monolayer cells prepared in advance and incubated for 24 hours, followed by replacement of 100 μL of the culture supernatant with an equal volume of fluorescent substrate, incubation for 2 minutes at room temperature, and incubation of 150 μL. The lysate was transferred to a new 96-well plate, the fluorescence value was measured, and the pseudovirus neutralization activity was calculated (expressed as IC50 ).
結果：
抗体２０Ｄ８のシュードウイルスに対する中和試験の検出結果は図４であり、野生株（
ＷＴ）と、変異株Ｐ．１株、Ｂ．１．３５１株、Ｂ．１．１．７株シュードウイルスに対
する中和活性のＩＣ５０はそれぞれ次のとおりであった。
野生株（ＷＴ）：１．３７ｎｇ／ｍＬ（図４のＡ）、
変異株Ｐ．１株：０．５６ｎｇ／ｍＬ（図４のＡ）、
変異株Ｂ．１．３５１株：０．４７ｎｇ／ｍＬ（図４のＢ）、
変異株Ｂ．１．１．７株：０．９５ｎｇ／ｍＬ（図４のＣ）。
result:
The results of the neutralization test of the antibody 20D8 against the pseudovirus are shown in FIG.
WT) and the mutant P. 1 strain, B.I. 1.351 strain, B. The IC50 of neutralizing activity against 1.1.7 strain pseudovirus was as follows.
Wild strain (WT): 1.37 ng/mL (A in FIG. 4),
Mutant P. 1 strain: 0.56 ng / mL (A in FIG. 4),
Mutant strain B. 1.351 strain: 0.47 ng/mL (B in FIG. 4),
Mutant strain B. 1.1.7 strain: 0.95 ng/mL (C in FIG. 4).
したがって、当該モノクローナル抗体はＳＡＲＳ－ＣｏＶ－２野生株、変異株にいずれ
も中和活性を有している。
Therefore, the monoclonal antibody has neutralizing activity against both wild type and mutant strains of SARS-CoV-2.
なお、上記の実施例は当業者が本発明の趣旨に対する理解を促すためのものに過ぎず、
本発明の保護範囲を限定するものではない。
It should be noted that the above examples are merely for promoting the understanding of the spirit of the present invention by those skilled in the art.
It does not limit the protection scope of the present invention.
Claims (12)
（１）配列番号１のアミノ酸配列の重鎖ＣＤＲ１（ＶＨＣＤＲ１）と、
（２）配列番号２のアミノ酸配列の重鎖ＣＤＲ２（ＶＨＣＤＲ２）と、
（３）配列番号３のアミノ酸配列の重鎖ＣＤＲ３（ＶＨＣＤＲ３）と、
（４）配列番号４のアミノ酸配列の軽鎖ＣＤＲ１（ＶＬＣＤＲ１）と、
（５）配列番号５のアミノ酸配列の軽鎖ＣＤＲ２（ＶＬＣＤＲ２）と、
（６）配列番号６のアミノ酸配列の軽鎖ＣＤＲ３（ＶＬＣＤＲ３）とであることを特徴
とするＳＡＲＳ－ＣｏＶ－２ウイルスに対するモノクローナル抗体２０Ｄ８。 The six CDR regions are
(1) heavy chain CDR1 (VHCDR1) of the amino acid sequence of SEQ ID NO: 1;
(2) a heavy chain CDR2 (VHCDR2) of the amino acid sequence of SEQ ID NO:2;
(3) a heavy chain CDR3 (VHCDR3) of the amino acid sequence of SEQ ID NO:3;
(4) a light chain CDR1 (VLCDR1) of the amino acid sequence of SEQ ID NO: 4;
(5) a light chain CDR2 (VLCDR2) of the amino acid sequence of SEQ ID NO:5;
(6) the monoclonal antibody 20D8 against the SARS-CoV-2 virus characterized by having the light chain CDR3 (VLCDR3) of the amino acid sequence of SEQ ID NO:6;
前記モノクローナル抗体２０Ｄ８の軽鎖可変領域のアミノ酸配列が配列番号８であるこ
とを特徴とする請求項１に記載の抗体。 the amino acid sequence of the heavy chain variable region of the monoclonal antibody 20D8 is SEQ ID NO: 7;
2. The antibody of claim 1, wherein the amino acid sequence of the light chain variable region of said monoclonal antibody 20D8 is SEQ ID NO:8.
２に記載の抗体。 Antibody according to claim 1 or 2, characterized in that said monoclonal antibody 20D8 is an IgG type antibody.
．３５１株、Ｂ．１．１．７株を含む複数のＳＡＲＳ－ＣｏＶ－２変異株を中和すること
ができることを特徴とする請求項１又は２に記載の抗体。 The monoclonal antibody 20D8 is wild-type SARS-CoV-2 and P. 1 strain, B.I. 1
. 351 strain, B. 3. The antibody of claim 1 or 2, wherein the antibody is capable of neutralizing multiple SARS-CoV-2 variants, including strain 1.1.7.
イルスのスパイクタンパク質Ｓ１であり、且つ、Ｄ６１４Ｇ、Ｋ４１７Ｔ／Ｎ、Ｅ４８４
Ｋ、Ｎ５０１Ｙのいずれか１つ又は複数の変異を含むＳＡＲＳ－ＣｏＶ－２のスパイクタ
ンパク質Ｓ１に結合することができることを特徴とする請求項１又は２に記載の抗体。 The antigen structural region bound by the monoclonal antibody 20D8 is the spike protein S1 of the SARS-CoV-2 virus, and D614G, K417T/N, E484
Antibody according to claim 1 or 2, characterized in that it is capable of binding to spike protein S1 of SARS-CoV-2 comprising any one or more mutations of K, N501Y.
１項に記載のモノクローナル抗体２０Ｄ８の使用。 Use of monoclonal antibody 20D8 according to any one of claims 1 to 5 in the manufacture of reagents for detecting SARS-CoV-2 virus.
Ｓ－ＣｏＶ－２ウイルスＢ．１．３５１株、ＳＡＲＳ－ＣｏＶ－２ウイルスＢ．１．１．
７株であることを特徴とする請求項７に記載の使用。 Said SARS-CoV-2 virus is the SARS-CoV-2 virus P. 1 share, SAR
S-CoV-2 virus B. 1.351 strain, SARS-CoV-2 virus B. 1.1.
8. Use according to claim 7, characterized in that there are 7 strains.
１項に記載のモノクローナル抗体２０Ｄ８の使用。 Use of monoclonal antibody 20D8 according to any one of claims 1-5 in the manufacture of a reagent that inhibits SARS-CoV-2 virus.
Ｓ－ＣｏＶ－２ウイルスＢ．１．３５１株、ＳＡＲＳ－ＣｏＶ－２ウイルスＢ．１．１．
７株であることを特徴とする請求項９に記載の使用。 Said SARS-CoV-2 virus is the SARS-CoV-2 virus P. 1 share, SAR
S-CoV-2 virus B. 1.351 strain, SARS-CoV-2 virus B. 1.1.
10. Use according to claim 9, characterized in that there are 7 strains.
品の製造における請求項１～５のいずれか１項に記載のモノクローナル抗体２０Ｄ８の使
用。 Use of monoclonal antibody 20D8 according to any one of claims 1 to 5 in the manufacture of a medicament for preventing and/or treating diseases caused by infection with SARS-CoV-2 virus.
Ｓ－ＣｏＶ－２ウイルスＢ．１．３５１株、ＳＡＲＳ－ＣｏＶ－２ウイルスＢ．１．１．
７株であることを特徴とする請求項１１に記載の使用。 Said SARS-CoV-2 virus is the SARS-CoV-2 virus P. 1 share, SAR
S-CoV-2 virus B. 1.351 strain, SARS-CoV-2 virus B. 1.1.
12. Use according to claim 11, characterized in that there are 7 strains.
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