JP2023509127A - Pipetting Devices, Pipette Tip Couplers, and Pipette Tips: Devices and Methods - Google Patents

Abstract

A pipette assembly includes a pipette device, a pipette tip, a leaf spring coupling device coupling the tip to the pipette device, and a distal elastomeric element (such as an O-ring), the coupling device comprising a stabilizer plateau. The stabilizer plateau includes a plurality of circumferentially disposed elements or segments in the form of flexible leaf springs that hold the pipette tip to the coupler and allow the pipette tip to rock on the coupler. The pipette tip includes dual complementary working surfaces within the pipette tip to prevent the distally directed axial stop surface of the pipette tip coupler from separating from the pipette tip coupler and the disposable pipette. It provides precise control of the axial coupling position, defined as the axial distance to the end of the pipette tip that contacts the liquid when the tip is in the coupled configuration.

Description

TECHNICAL FIELD The present disclosure relates generally to pipetting devices, and more specifically to pipette tip couplers, disposable pipette tips, pipette tip and coupler combinations, and at least one pipette tip operably carried by a pipette device. A method of connecting and disconnecting at least one disposable pipette tip to or from a coupler.

Pipette devices are used in many industries for the transfer of liquids to perform experimental analyses. As such, disposable pipette tips are used to provide control within the experiment being conducted and are intended for one-time use. A disposable pipette tip is a manual pipetting device having multiple pipette units arranged in rows or columns for aspirating samples from multiple containers simultaneously and dispensing them to other locations. and in automated pipetting devices.

Disposable pipette tips have historically been constructed to interface with either conical or stepped coupling studs. In cases where a conical coupling stud is used, the disposable pipette tip is constructed in such a way that it must be prestressed over the coupling stud to provide an airtight seal. Due to the tolerances of the two interfacing components, the distance to the end of the pipette tip that comes into contact with the liquid is not well controlled. In addition, a high pressing force is required to prestress the pipette tip and create an airtight seal. As a result, microcracks can form in the pipette tip, which causes leakage. Moreover, the high pressing force during installation of the pipette tip has the disadvantage that a correspondingly high force must be applied for releasing the pipette tip.

The assignee of the present application (HAMILTON Company), in U.S. Pat. No. 7,033,543, issued Apr. 25, 2006, teaches a stepped coupling stud with an O-ring, which It provides a solution for reducing the high pressing force required to create an airtight seal and also provides a well-defined axial positioning of the end of the pipette tip that comes into contact with the liquid. Provide solutions to provide. When the O-ring is compressed, it not only provides an axially directed force, providing an airtight seal, but also acts as an axial coupling feature on the coupling stud, on the pipette tip. to engage opposite axial coupling features of the .

Nonetheless, current systems utilizing stepped coupling studs and a single O-ring configuration result in failure of the hermetic seal and degradation of pipette device performance when the O-ring is compromised. Therefore, there are many problems.

Additionally, compression of the O-ring results in deformation of the O-ring, which provides an axially directed force and an airtight seal against the working surface of the pipette tip. Reversing this action, when compression of the O-ring is removed, the O-ring disengages from the working surface of the pipette tip, allowing the pipette tip to be removed from the coupling stud and pipette device for disposal. must be If the O-ring is not fully depressurized, some residual force will remain, resulting in the pipette tip remaining engaged to the coupling stud and thus the pipette tip for disposal. requires an automated external axial reaction force to remove the

Moreover, as the size of the pores (into and/or through which liquids are transferred) decreases, the need to precisely position all of the pipette tips in a controlled manner increases for successful targeting. do.

Accordingly, there is a need to ameliorate or overcome one or more of the significant shortcomings delineated above.

Accordingly, in one aspect, embodiments of the present disclosure provide a combined pipette tip coupler and disposable pipette tip that includes a plurality of circumferentially disposed elements or segments, thereby improving the performance of the known prior art. Ameliorating or overcoming one or more of the disadvantages, the plurality of circumferentially-disposed elements or segments comprise: Engaging a circumferential inner working surface defining a first working surface formed into an interior circular scribing surface of a sidewall of the pipette tip and resulting in a preform. A stressing force is adapted to prestress the pipette tip axially upwardly and the distal elastomeric element of the coupler prestresses against the second internal working surface of the pipette tip. to create a seal arrangement that eliminates known prior art seal degradation or failure.

Additionally and in one aspect, the distal elastomeric element provides an opposite axial force to the plurality of elements or segments when compressed against the second internal work surface, and this At least one benefit of the opposing axial forces is when multiple individual elements or segments are radially and axially interfering to provide a stronger distal seal. Second, additional force is applied to the first work surface by a plurality of individual elements or segments.

A further benefit of the reversed axial force is that when multiple individual elements or segments are disengaged to a radially retracted condition, the reversed axial force of the distal elastomeric element is defines an axially directed disengagement force, which assists in removing the pipette tip from the pipette tip coupler for disposal.

In another aspect, embodiments of the present disclosure provide a combination pipette tip coupler and disposable pipette tip, wherein the coupler includes, but is not limited to, a plurality of circumferentially disposed elements or segments and an O-ring. and the pipette tip includes dual complementary internal working surfaces within the pipette tip to provide the resulting axial force, and the resulting axial force is realized from engagement of a plurality of elements or segments and a distal elastomeric element with dual complementary working surfaces to prestress the disposable pipette tip into an axially coupled position; Axial coupling locations are provided by a distally facing axial stop surface on the pipette tip coupler and a complementary opposite axial stop surface on the proximally facing disposable pipette tip, with vertical datums on the pipette. It is adapted to be established with respect to the longitudinal axis of the channel of the pipette device carrying the tip coupler and disposable pipette tip combination, which provides straightness and controlled concentricity of the pipette tip.

Therefore, one benefit of the resulting axial force coupling location over the known prior art is the establishment of this vertical datum that provides straightness and controlled concentricity of the pipette tip. Concentricity worsens as the angle, defined herein as "φ", between the transverse axis and the longitudinal axis perpendicular to the transverse axis is allowed to increase. Therefore, controlled concentricity is especially important for multi-channel systems and targeting multiple wells. Therefore, the pipette tip coupler and disposable pipette tip combination provides tighter concentricity, allowing tighter precision of all of the pipette tips in a controlled manner, multiple wells and/or smaller holes. (to which liquid is transferred and/or from) is successfully targeted.

In another aspect, embodiments of the present disclosure provide a combination pipette tip coupler and disposable pipette tip, wherein the coupler includes, but is not limited to, a plurality of circumferentially disposed elements or segments and an O-ring. and the pipette tip includes dual complementary working surfaces within the pipette tip to provide precise control of axial coupling position and axial coupling is measured as the axial distance from the distally-facing axial stop surface of the pipette tip coupler to the end of the pipette tip that contacts liquid when the pipette tip coupler and disposable pipette tip are in the coupled configuration. Defined. This, combined with pipette tip straightness, allows pipette devices carrying pipette tip couplers and disposable pipette tip combinations to target smaller bores. Additionally, smaller volumes of liquid can be transferred, resulting from the known fixed distance of the disposable pipette tip, to the work surface onto which or from which the liquid will be transferred. Allows for controlled pipette tip/liquid contact.

In yet another aspect, embodiments of the present disclosure provide pipette tip couplers and disposables that include an angled squeeze mechanism that directs movement of a plurality of individual elements into contact with a first work surface of the pipette tip. Offers a combination of pipette tips. As a result, the axial force is greater to pre-stress the pipette tip into the axial coupling position.

In yet another aspect, embodiments of the present disclosure provide a combined pipette tip coupler and disposable pipette tip, the coupler comprising a flexible leaf spring with retention bumps to retain the pipette tip to the coupler. a plurality of circumferentially disposed elements or segments in the form of a stabilizer plateau to prevent the pipette tip from rocking over the coupler; and a distal elastomer in the form of, but not limited to, an O-ring. and the pipette tip includes dual complementary working surfaces within the pipette tip to provide precise control of the axially articulated position, the axially articulated position being controlled by the pipette It is defined as the axial distance from the distally facing axial stop surface of the tip coupler to the end of the pipette tip that contacts liquid when the pipette tip coupler and disposable pipette tip are in the coupled configuration.

Further aspects of embodiments of the present disclosure will become apparent from the detailed description provided below when taken in conjunction with the accompanying drawings and claims. However, numerous modifications and adaptations can be made without departing from the scope and fair meaning of the claims as set forth below following the detailed description of the preferred embodiment of the disclosure. should be understood.

The foregoing general description, and the following detailed description of the disclosure, may be more fully understood with reference to the following drawings, which are for illustrative purposes only and illustrate the scope of the disclosure. is not intended to limit Also, it can be appreciated that the drawings are not necessarily to scale. The reason is that some components are shown enlarged or out of proportion to their size in an actual implementation in order to more clearly illustrate one or more concepts of this disclosure. This is because there is a possibility that

FIG. 11 is a perspective view of an exemplary embodiment of an air displacement pipette device assembly of an automated liquid handling system; FIG. 11 is a longitudinal cross-sectional side view of an exemplary embodiment of a pipette device assembly; Pipette device assembly including an expandable mandrel collet coupling device operably coupled to an exemplary embodiment of a disposable pipette tip or a pipette device operably coupled to an exemplary embodiment of a pipette tip coupler 1 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment; FIG. FIG. 10A is a side view of an exemplary embodiment of a pipette device assembly; FIG. 10 is a partially exploded perspective view of a pipetting device assembly detailing the components of an exemplary embodiment of an expandable mandrel collet coupling device; FIG. 10 is a fragmentary, partially exploded perspective view detailing components of an exemplary embodiment of an expanding mandrel collet coupling device interposed between a disposable pipette tip and a pipetting device; FIG. 12A is a side view of an exemplary embodiment of an expanding mandrel collet coupling device; FIG. 10 is a top and side perspective view of the center coupler body of the expanding mandrel collet coupling device; [0014] FIG. 4A is a top and side perspective view of an exemplary embodiment of a lower or distal elastomeric element or O-ring of an exemplary embodiment of an expandable mandrel collet coupling device; a distal elastomeric element surrounding the distal stem portion of the central coupler body, and an upper surface of a cylindrical spacer mounted thereon surrounding and surrounding the central body axially above the distal elastomeric element; It is a side perspective view. FIG. 12A is a top and side perspective view of an exemplary embodiment of an expanding mandrel collet of an expanding mandrel collet coupling device; FIG. 10 is a side perspective view in longitudinal section of an exemplary embodiment of an expanding mandrel collet of an expanding mandrel collet coupling device; FIG. 12A is a top and side perspective view of an exemplary embodiment of an annular wedge of an exemplary embodiment of an expandable mandrel collet coupling device; FIG. 10 is a side view of an exemplary embodiment of an expanding mandrel collet in an expanded configuration with application of force from a fragmentary illustrated piston sleeve or squeeze sleeve; FIG. 10 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of an expanding mandrel collet of an expanding mandrel collet coupling device operably coupled to a pipetting device; FIG. 12A is a fragmentary, partially cross-sectional side view of an exemplary embodiment of a disposable pipette tip operably coupled to a pipette device as an embodiment of an expandable mandrel collet coupling device; FIG. 10A is a side view of an exemplary embodiment of a disposable pipette tip shown in a supported position; FIG. 10 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of a disposable pipette tip detailing the interior of the disposable pipette tip; FIG. 10 is a fragmentary longitudinal cross-sectional side view of the upper coupling portion of an exemplary embodiment of a disposable pipette tip detailing the interior of the upper coupling of the disposable pipette tip; 1 is a schematic block diagram illustration of an exemplary embodiment of an automated pipetting workstation or system; FIG. FIG. 10 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of a pipetting device supporting an exemplary embodiment of an expandable mandrel collet coupling device over a disposable pipette tip; FIG. 10 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of an expanding mandrel collet coupling device positioned over and into a disposable pipette tip defining a coupling stage; The elastomeric element is in initial contact with the sealing seat surface of the pipette tip and the plurality of individual coupling elements or segments are in an uncompressed or non-extending radially outward state to provide sealing. FIG. 10 is a view in which the seat surface has a sharp sealing seat surface angle with respect to the central longitudinal axis of the pipette tip; FIG. 12B is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of an expanding mandrel collet coupling device and pipette tip showing a plurality of expansions against the upper corners of a groove formed in the pipette tip. The pipette tip is lifted as a result of the piston sleeve being pushed down over the annular wedge to radially extend the rounded portion of the mandrel collet segment, which either lifts the pipette tip or FIG. 12 Resulting in a pulling axial force, which initiates the process of seating the pipette tip and compressing the distal elastomeric element against the sealing seat surface of the pipette tip. Of the expanding mandrel collet segment arms of the expanding mandrel collet of the segmented coupler extended to contact the corners of the pipette tip groove, as illustrated in FIG. 1 is a side detail view in fragmentary longitudinal section of one rounded surface of the . FIG. 24 is a fragmentary longitudinal cross-sectional side detail view of a distal elastomeric element in initial compression against a sealing seat surface of a pipette tip, as illustrated in FIG. 23; FIG. 12B is a fragmentary longitudinal cross-sectional side view of the exemplary embodiment of the expanding mandrel collet coupling device positioned further into the pipette tip, with the pipette tip raised while the piston sleeve is annular; to continue to extend the rounded surfaces of the multiple expanding mandrel collet segments radially into the grooves of the pipette tip, pulling the pipette tip further up and removing the sealing seat of the pipette tip. FIG. 11 shows further compression of the distal elastomeric element against the surface. Fragmentary longitudinal cross-sectional side detail of the rounded surface of one of the expanding mandrel collet segments extending further into the groove of the pipette tip, as illustrated in FIG. It is a diagram. FIG. 27 is a fragmentary longitudinal cross-sectional side detail view of the distal elastomeric element being further compressed from its initial compression state against the sealing seat surface of the pipette tip, as illustrated in FIG. 26; FIG. 12B is a fragmentary longitudinal cross-sectional side view of the exemplary embodiment of the expanding mandrel collet coupling device positioned in a disposable pipette tip with the annular wedge moved to its final position; , the pipette tip has been lifted to its final seated condition, thereby defining its final coupling condition, and the distal elastomeric element is in its final seating position against the sealing seat surface of the pipette tip. FIG. 12 is in a compressed, seated, sealing state; A fragmentary longitudinal cross-section of a rounded surface of one of a plurality of expanding mandrel collet segments extending into abutment with a surface defining a groove, as illustrated in FIG. It is a detailed side view. FIG. 30 is a fragmentary longitudinal cross-sectional side detail view of the distal elastomeric element in its final compressed and seated sealing condition against the sealing seat surface of the pipette tip, as illustrated in FIG. 29; FIG. 10B is a fragmentary longitudinal cross-sectional side detail view of the beginning of the connection between an exemplary embodiment of an expanding mandrel collet coupling device and an exemplary embodiment of a disposable pipette tip, with an illustration of the forces involved; . One of a plurality of arcuate or rounded segment surfaces of one of a plurality of expanding mandrel collet segments and grooves of an exemplary embodiment of a disposable pipette tip with an illustration of the forces involved. Fig. 3 is a side detail view in fragmentary longitudinal section of the beginning of the connection of the . FIG. 11 is a fragmentary longitudinal cross-sectional side detail view of the fully mated state between an exemplary embodiment of an expanding mandrel collet coupling device and an exemplary embodiment of a disposable pipette tip, with an illustration of the forces involved; be. Fig. 10 is a fragmentary longitudinal section illustrating a misaligned coupling between an exemplary embodiment of an expandable mandrel collet coupling device and an exemplary embodiment of a disposable pipette tip to define a misalignment parameter; It is a side view. A pipette operably coupled to a misaligned coupling between an exemplary embodiment of an expandable mandrel collet coupling device and an exemplary embodiment of a disposable pipette tip to define a misalignment parameter. FIG. 2 is a fragmented and cutaway longitudinal cross-sectional side view of an embodiment of a device; FIG. 12A is a fragmented and cutaway, longitudinal cross-sectional side view of an exemplary embodiment of an air displacement pipette device coupled to an expandable mandrel collet coupling device, the expandable mandrel collet coupling device being , is connected to a disposable pipette tip having a small volume of liquid interposed between the end of the pipette tip and the work surface, this figure being shown and identified. FIG. 10 further has dimension lines; FIG. 2 is a fragmentary longitudinal cross-sectional side view detailing the interior of an exemplary embodiment of a disposable pipette tip, the view further having dimension lines shown and identified; Fragment of an exemplary embodiment of a pipetting device operably coupled to an exemplary embodiment of an expandable mandrel collet coupling device, with dimension lines relative to the dimension lines in FIG. 38 shown and identified. 1 is a side view of a typical longitudinal section; FIG. 1 is a longitudinal side view of a pipette device assembly illustrating a circuit board processing signals from liquid level detection (LLD) circuit contacts connected between the circuit board and a squeeze sleeve; FIG. , the squeeze sleeve is in contact with a plurality of segments or elements that connect with the pipette tip via an annular wedge, the distal end of the pipette tip being shown in contact with the liquid; is. Illustration of an expandable mandrel collet coupling device positioned above an exemplary embodiment of a disposable pipette tip including an alternative sealing seat surface angle of substantially 90 degrees with respect to the central longitudinal axis of the pipette tip. 1 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment; FIG. FIG. 12 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of an expandable mandrel collet coupling device positioned in a disposable pipette tip including an alternative sealing seat surface angle of substantially 90 degrees; The pipette tip has been lifted to its final seated condition, the annular wedge has been moved to its final position to define the final coupling condition, and the distal FIG. 11 shows the elastomeric element in its final compressed and seated sealing condition for an alternative sealing seat surface angle of substantially 90 degrees; FIG. 42 is a fragmentary longitudinal cross-sectional side detail view of the distal elastomeric element in final compression for an alternative sealing seat surface angle of substantially 90 degrees; be. Fragmentary of an exemplary embodiment of a disposable pipette tip illustrating details of the upper interior of the disposable pipette tip including another alternative sealing seat surface in the form of a circumferentially radially concave sealing seat surface. It is a side view of a longitudinal section. 45 is a fragmentary longitudinal cross-sectional side detail view of the exemplary embodiment of the disposable pipette tip illustrating details of the circumferentially radially concave sealing seat surface illustrated in FIG. 44; FIG. FIG. 10 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of a disposable pipette tip illustrating details of a further alternative sealing seat surface in the form of a circumferential radially convex sealing seat surface; 47 is a fragmentary longitudinal cross-sectional side detail view of the exemplary embodiment of the disposable pipette tip illustrating details of the circumferential radially convex sealing seat surface illustrated in FIG. 46; FIG. FIG. 10 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of a disposable pipette tip illustrating a still further alternative sealing sheet surface in the form of a circumferentially upwardly facing tooth edge sealing sheet surface; FIG. 49 is a fragmentary longitudinal cross-sectional side detail view of the exemplary embodiment of the disposable pipette tip, illustrating details of the circumferentially upward facing edge sealing sheet surface illustrated in FIG. 48; An alternative V defined by a V-shaped circumferential inner surface of a disposable pipette tip that opens toward the longitudinal axis and has a V-shaped cross-section as shown. FIG. 12A is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of an expanding mandrel collet coupling device positioned above an exemplary embodiment of a disposable pipette tip including a letter-shaped groove. FIG. 12A is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of an expanding mandrel collet coupling device positioned in a disposable pipette tip including an alternative V-shaped groove, the pipette tip extending into the pipette tip; Raised to its final condition, the rounded surfaces of the expanding mandrel collet segments extend into the V-shaped grooves, and the V-shaped circumference FIG. 12 abuts the inner surface of the direction, with the distal elastomeric element in a final compressed and seated sealing condition against the sealing seat surface of the pipette tip. As shown in FIG. 51, a plurality of V-shaped grooves extending into the V-shaped groove and abutting a circumferential inner surface of the V-shaped groove defining the V-shaped groove. 1 is a side detail view in fragmentary longitudinal section of the rounded surface of one of the expanding mandrel collet segments of FIG. FIG. 12B is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of an expanding mandrel collet coupling device positioned above a second exemplary embodiment of a disposable pipette tip; FIG. 10 is a side detail view in fragmentary longitudinal section detailing the interior of a second exemplary embodiment of a disposable pipette tip; FIG. 12B is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of an expanding mandrel collet coupling device positioned within a second exemplary embodiment of a disposable pipette tip, and a stop disk of the coupling device; The shoulder surface abuts the axial stop surface of the second exemplary embodiment of the disposable pipette tip, and the rounded surface of the plurality of expandable mandrel collet segments abuts the second exemplary embodiment of the disposable pipette tip. A second exemplary embodiment of a disposable pipette tip wherein the distal elastomeric element extends against the inner surface of the enclosing sidewall of the exemplary embodiment resulting in deformation of the inner surface. 1 is in its final compressed and seated sealing condition against the sealing seat surface of FIG. An expanding mandrel collet coupling that extends against and deforms the inner surface of the surrounding sidewall of the second exemplary embodiment of the disposable pipette tip, as illustrated in FIG. FIG. 4 is a side detail view in fragmentary longitudinal section of the rounded surface of one of the expanding mandrel collet segments of the device; 20 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of a disposable pipette tip including an alternative groove shape embodiment to at least the circumferentially annular tip groove illustrated in FIG. 19; FIG. 20 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of a disposable pipette tip including an alternative groove shape embodiment to at least the circumferentially annular tip groove illustrated in FIG. 19; FIG. 20 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of a disposable pipette tip including an alternative groove shape embodiment to at least the circumferentially annular tip groove illustrated in FIG. 19; FIG. 20 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of a disposable pipette tip including an alternative groove shape embodiment to at least the circumferentially annular tip groove illustrated in FIG. 19; FIG. 20 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of a disposable pipette tip including an alternative groove shape embodiment to at least the circumferentially annular tip groove illustrated in FIG. 19; FIG. 20 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of a disposable pipette tip including an alternative groove shape embodiment to at least the circumferentially annular tip groove illustrated in FIG. 19; FIG. 20 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of a disposable pipette tip including an alternative groove shape embodiment to at least the circumferentially annular tip groove illustrated in FIG. 19; FIG. 20 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of a disposable pipette tip including an alternative groove shape embodiment to at least the circumferentially annular tip groove illustrated in FIG. 19; FIG. 20 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of a disposable pipette tip including an alternative groove shape embodiment to at least the circumferentially annular tip groove illustrated in FIG. 19; FIG. 20 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of a disposable pipette tip including an alternative groove shape embodiment to at least the circumferentially annular tip groove illustrated in FIG. 19; FIG. 20 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of a disposable pipette tip including an alternative groove shape embodiment to at least the circumferentially annular tip groove illustrated in FIG. 19; FIG. FIG. 12B is a top and side perspective view of a second or alternative exemplary embodiment of the expanding mandrel collet of the expanding mandrel collet coupling device; FIG. 4B is a side perspective view in longitudinal section of a second or alternative exemplary embodiment of the expanding mandrel collet of the expanding mandrel collet coupling device; FIG. 11 is a perspective view of an alternative exemplary embodiment of an air displacement pipette device assembly of an automated liquid handling system; FIG. 11 is a side view in longitudinal section of one side of an alternative exemplary embodiment of a pipetting device assembly; FIG. 11 is a fragmentary longitudinal cross-sectional side view of another side of an alternative exemplary embodiment of a pipetting device assembly; 73 is a partial exploded view of the pipetting device assembly detailing the parts of the pipetting device illustrated in FIG. 72; FIG. FIG. 4 is a partially exploded perspective view of a pipette device assembly detailing the parts of an exemplary embodiment of a nozzle and leaf spring coupling device; FIG. 10 is a fragmentary, partially exploded perspective view detailing parts of an exemplary embodiment of a nozzle and leaf spring coupling device interposed between a disposable pipette tip and a pipette device; FIG. 12A is a side view of an exemplary embodiment of a leaf spring coupling device; FIG. 12A is a top and side perspective view of an exemplary embodiment of a leaf spring coupling device; FIG. 12A is a top and side perspective view of an exemplary embodiment of a lower or distal elastomeric element or O-ring of an exemplary embodiment of a leaf spring coupling device; FIG. 10 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of a nozzle and leaf spring coupling device operably connected to a pipetting device; FIG. 10 is a fragmentary, partially cross-sectional side view of an exemplary embodiment of a disposable pipette tip operably coupled to a pipette device as an embodiment of a nozzle and leaf spring coupling device; FIG. 10 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of a pipette device supporting an exemplary embodiment of a nozzle and leaf spring coupling device above a disposable pipette tip; FIG. 10 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of a nozzle and leaf spring coupling device positioned on and in a disposable pipette tip defining a coupling stage; The leaf spring is compressed, the retention bump is beginning to enter the groove of the pipette tip, the distal elastomeric element is in first contact with the sealing seat surface of the pipette tip, and the sealing seat surface of the pipette tip. FIG. 10 has a sharp sealing seat surface angle with respect to the central longitudinal axis; A rounded surface of one of a plurality of retention bumps of a leaf spring of a leaf spring coupling device in contact with a corner of a groove of a pipette tip, as illustrated in FIG. 1 is a side detail view in fragmentary longitudinal section of the . FIG. 83 is a side detail view in fragmentary longitudinal section of the distal elastomeric element in initial contact with the sealing seat surface of the pipette tip, as illustrated in FIG. 82; FIG. 12B is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of a nozzle, leaf spring coupling device, and pipette tip, with the leaf spring retention bump snapped into the groove of the pipette tip, and the distal FIG. 11 shows the elastomeric element seated in compression against the sealing seat surface of the pipette tip; Fragmentary beginning of the connection between one of a plurality of arcuate or rounded segment surfaces of a retention bump of a leaf spring and a groove of an exemplary embodiment of a disposable pipette tip, with an illustration of the forces involved. is a detailed side view of a longitudinal section; Fragmentary beginning of the connection between one of a plurality of arcuate or rounded segment surfaces of a retention bump of a leaf spring and a groove of an exemplary embodiment of a disposable pipette tip, with an illustration of the forces involved. is a detailed side view of a longitudinal section; FIG. 10 is a fragmentary longitudinal cross-sectional side detail view of the full coupling between an exemplary embodiment of a leaf spring coupling device and an exemplary embodiment of a disposable pipette tip, with an illustration of the forces involved; FIG. 10 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of a pipetting device operably coupled to an exemplary embodiment of a leaf spring coupling device, with the Z-axis indicated; FIG. 12B is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of a leaf spring coupling device positioned in a disposable pipette tip including an alternative sealing seat surface angle of substantially 90 degrees, the pipette tip; has been lifted to its final seated condition and the distal elastomeric element is in its final compressed, seated sealing condition against an alternative sealing seat surface angle of 90 degrees. is. FIG. 91 is a fragmentary longitudinal cross-sectional side detail view of a distal elastomeric element in final compression for an alternative sealing seat surface angle of 90 degrees, as illustrated in FIG. 90;

For purposes of illustrating this disclosure, presently preferred embodiments are shown in the drawings. These exemplary embodiments will now be described more fully with reference to the accompanying drawings, wherein like reference numerals refer to like parts throughout the description of the several figures of the drawings. or used to indicate a part.

Pipette assembly with expanding mandrel collet coupling and tip

1 and 2 illustrate an exemplary embodiment of a pipette device assembly 10, which includes an exemplary embodiment of a pipette device 20 and an exemplary expandable mandrel collet coupling device 100 or pipette tip coupler. and an exemplary embodiment of a disposable pipette tip 220 removably coupled to pipette device 20 as expandable mandrel collet coupling device 100 .

Pipette device 20

Referring to FIG. 2, the pipetting device 20 includes a body portion 22 that supports an aspiration and dispensing device 24 that includes a plunger 26 and a plunger 26. 26 is operatively connected to and driven by motor 28 . The plunger 26 resides within a plunger cylinder 30 that extends from the distal (tip) or lower end 32 of the body portion 22 of the pipette device 20 . .

Pipette device 20 further includes an aspiration and dispensing cylinder 34 that is positioned axially aligned with plunger 26 and distally below plunger 26 . 30 at least partially. Aspiration and dispensing cylinder 34 transitions distally to distal mounting flange 36 for attachment to expandable mandrel collet coupling device 100 , and expandable mandrel collet coupling device 100 includes disposable pipette tip 220 . and removably connect with

1, 3, and 15, aspiration and dispensing cylinder 34 further includes an inner enclosing sidewall 38 having an open-ended pipette channel 40 extending therethrough. is defined. An open-ended pipette channel 40 is positioned at the open upper end of the aspiration and dispensing cylinder 34 to provide open communication between the plunger 26 and the external area adjacent the distal mounting flange 36. Extending longitudinally along the longitudinal channel axis 80 of the pipetting device assembly 10 between the portion 42 and the open lower end portion 44, the distal mounting flange 36 includes an expandable mandrel collet cup. It is operably connected to the central body member 102 of the ring device 100, the central body member 102 including an open-ended central channel 136 extending through the central body member 102 and having an expandable mandrel collet cup. An open communication is provided between the tip 220 and the aspiration and dispensing cylinder 34 via the ring device 100 .

piston or squeeze sleeve 46

3 and 4, pipette device 20 further includes a hollow piston or squeeze sleeve 46 that has a proximal or upper end 48 and a distal or lower end 50. have. A squeeze sleeve 46 surrounds both the plunger cylinder 30 and the aspiration and dispensing cylinder 34 and is operatively connected to the squeeze motor 52 .

As shown in FIG. 4, the squeeze motor 52 of the pipetting device assembly 10 is supported on the body portion 22 of the device 20 and is operatively connected to the lead screw 54 to force the lead screw 54 to move. The driving and lead screw 54 is connected to an axially translating lead nut 56 which is operatively connected to a squeeze linkage 58 . Squeeze linkage 58 is operably connected to the proximal or upper end 48 of squeeze sleeve 46 via squeeze linkage arm 60 such that rotation of squeeze motor 52 in the first direction is longitudinal. It is adapted to result in linear axial translation of squeeze sleeve 46 in a distal or vertically downward direction along channel axis 80 (FIG. 3) and in a second or opposite direction. Subsequent rotation of the squeeze motor 52 causes linear opposite axial translation of the squeeze sleeve 46 along the longitudinal channel axis 80 (FIG. 3) in a proximal direction opposite a downward direction or in a vertically upward direction. It is designed to be produced as a result.

Ejection sleeve 62

Referring to FIG. 4, the pipette device 20 further includes an ejection sleeve 62, which is used to eject (remove) the disposable pipette tip 220 from the pipette device 20, the ejection sleeve 62: Axially movable relative to the aspiration and dispensing cylinder 34 (FIG. 2) and having a proximal or upper end 64, a distal or lower end 66, and an ejection sleeve arm 68. , and an ejection sleeve arm 68 is attached at a first end to the ejection sleeve 62 adjacent the upper end 64 and to a first end of the plunger device 70 . It has an opposing second end.

As shown in FIG. 5, the plunger device 70 includes opposed end surfaces 72 that abut one end of an ejection sleeve spring 74 to provide an ejection sleeve spring. The spring 74 has opposed spring ends that abut an upper surface portion 76 of the body portion 22 of the device 20 , and the ejection sleeve spring 74 extends between the surfaces 72 and 76 . and is spring loaded to bias the plunger device 70 and attached sleeve 62 in the normal pipette tip ejected condition.

As illustrated in FIG. 2, the normal pipette tip ejected condition is a force to overcome the ejection sleeve spring force (e.g., , connection to the pipette tip 220, etc.). FIG. 2 further illustrates that the spring 74 surrounds a central spring guide member 78 to retain the shape of the spring 74 and prevent the spring 74 from buckling.

Moreover, spring 74 is sized so that the force exerted by sleeve 62 on pipette tip 220 in the course of its relaxation is sufficient to assist in ejecting tip 220 from expandable mandrel collet coupling device 100 . It is

Note that the expandable mandrel collet coupling device 100 and disposable pipette tip 220 may be practiced in other embodiments of pipette devices, where the pipette device 20 embodiment is provided merely as an example and not as a limitation. should be recognized.

Expandable Mandrel Collet Coupling Device 100

5-7, the expandable mandrel collet coupling device 100 includes an elongated central body member 102; an expandable collet 170 configured to surround the elongated central body member 102 and including a segmented collar 200; and an annular wedge or washer 210.

Annular wedge 210 is configured to receive the upper portion of elongated central body member 102 therethrough so as to axially move over the interior of expandable collet 170 adjacent segmented collar 200 . , as a function of the axial location of the annular wedge 210 relative to the central body member 102, from an unexpanded state having a first perimeter to an expanded state having a second perimeter greater than the first perimeter. 21 to the interior of the pipette tip 220 as shown in FIG. 29. are adapted to engage.

Elongated central body member 102

More specifically, and with reference to FIGS. 7 and 8, expandable mandrel collet coupling device 100 includes an elongated central body member 102 extending along longitudinal central axis 90. extends between a proximal or upper annular end face 104 and a distal or lower annular end face 130 .

As shown in FIG. 8, the upper annular end face 104 of the central body member 102 includes an outer chamfered perimeter 106 that transitions into an elongated tubular upper shank member 108 that extends into the shank member. 108 transitions distally to annular tapered portion 110 . In one embodiment, shank member 108 is threaded for assembly with distal mounting flange 36, and distal mounting flange 36 has corresponding threads. Annular tapered portion 110 decreases in diameter from shank member 108 and transitions distally to cylindrical neck portion 112 . Cylindrical neck portion 112 merges distally into a cylindrical collar 114 having a diameter greater than the diameter of cylindrical neck portion 112 .

A lower cylindrical body member 120 follows the cylindrical collar 114 and has a diameter greater than the diameter of the cylindrical neck portion 112 . Body member 120 extends distally from cylindrical collar 114 to an upper annular shoulder end or stop surface 122 of distal cylindrical stem surface 124, which is , which is larger than the diameter of the lower cylindrical body member 120 .

8, distal cylindrical stem portion surface 124 transitions from upper annular shoulder end 122 to rounded end plate 126, where rounded end plate 126 has an upper surface 128 and a lower surface defined by a distal or lower annular end surface 130 . As shown, the end plate 126 has a diameter greater than the diameter of the stem portion surface 124 and the distal stem portion surface 124 is in the distal groove portion of the expandable mandrel collet coupling device 100. or defining a lower groove portion 132 .

8 and 15, the elongated central body member 102 includes an inner cylindrical channel surface 134 that defines an open-ended cylindrically shaped central channel or passageway 136. , which extends through the central body member 102 and between the upper annular end face 104 and the lower annular end face 130 along the central longitudinal axis 90 of the pipette device assembly 10. It is adapted to provide open channel communication through elongated central body member 102 to open-ended pipette channel 40 extending longitudinally along longitudinal channel axis 80 .

Distal elastomeric element 140

As further illustrated in FIG. 7, expandable mandrel collet coupling device 100 further includes a distal or lower elastomeric element 140, which is located at the distal end portion of elongated central body member 102. Coaxially carried.

In one embodiment, and referring to FIG. 9, distal elastomeric element 140 includes an annular body portion 142 . Annular body 142 includes an interior surface 144 defining a central opening 146 , a top surface 148 , a peripheral exterior surface 150 and a bottom surface 152 . The central opening 146 is sized to closely or closely surround the distal cylindrical stem portion 124 of the expandable mandrel collet coupling device 100, while, as illustrated in FIG. It is shaped to reside in groove 132 and extend circumferentially radially outward beyond end plate 126 . In a relaxed or uncompressed state, distal elastomeric element 140 includes a circumferentially continuous, generally circular cross-sectional area 154, as illustrated in FIG.

spacer 160

Referring to FIG. 10, the expandable mandrel collet coupling device 100 further includes a spacer 160 configured to surround the elongated central body member 102 or to extend in conjunction with the elongated central body member 102 . It is configured to be integrally formed. As shown, spacer 160 includes a cylindrically-shaped body portion 162 extending between upper and lower ends 164 and 165 . The cylindrical body portion 162 includes an interior surrounding surface 166 (Fig. 15) that defines an open-ended passageway 168 extending through the body portion 162, the passageway 168 being elongated. It is sized to closely or closely surround the lower cylindrical body member 120 of the central body member 102 .

7, 10 and 15, the spacer 160 is further configured to be surrounded by an expandable mandrel collet 170 with an upper end 164 of the spacer 160 connecting to the distal end of the mounting flange 36. and the inferior end 165 abuts the inner annular shoulder stop surface 177 of the annular base portion 172 of the expandable mandrel collet 170, which is attached to the distal annular end or lower side. Further includes an annular end 176 that extends into the distal cylindrical stem portion of elongate center body member 102 for coaxially securing expandable mandrel collet 170 to center body member 102 along longitudinal centerline axis 90 . It mounts over the distal annular shoulder stop surface 122 at 124 (FIG. 8).

15 and 16, and as described above, the shank member 108 of the expandable mandrel collet coupling device 100 fits within the distal mounting flange 36 of the aspiration and dispensing cylinder 34. and is adapted to operatively couple the expandable mandrel collet coupling device 100 to the pipette device 20, and as the expandable mandrel collet coupling device 100, the disposable pipette tip 220 is used for pipetting. It is adapted to be removably coupled to device 20 such that longitudinal channel axis 80 and central axis 90 form a coincident or common longitudinal channel axis.

Expandable mandrel collet 170

7 and 11, the expandable mandrel collet 170 includes a plurality of circumferentially spaced upwardly extending collet arms 180 that are attached to a lower annular base portion 172. From the flattened end 184 , there transitions upwardly to a segmented free end 200 which is disposed axially above the lower annular base portion 172 . defines a segmented color. A plurality of circumferentially spaced upwardly extending collet arms 180 are coupled by one of a plurality of circumferentially spaced upwardly extending slots 182 . separated from each other.

As shown in FIGS. 11 and 12, each of the plurality of upwardly extending collet arms 180 includes a respective lower arm portion 186, each lower arm portion 186 leading to a respective upper arm portion. We are transitioning to portion 190 . In one embodiment, a plurality of circumferentially-spaced lower arm portions 186 form a generally cylindrically-shaped surrounding lower body portion 181, and a plurality of Circumferentially spaced apart upper arm portions 190 of the body form a frusto-conically shaped surrounding upper body portion 183 that extends from the lower body portion 181 to the There is radial outward and upward transition. Lower body portion 181 may be configured with a slight upward taper or increased circumference relative to distal or lower annular base portion 172 .

base portion 172

11 and 12, distal or lower annular base portion 172 includes a distally facing or downwardly facing base surface 171 and a proximally facing or upwardly facing base surface 173 . Distal facing base surface 171 transitions downward to a shortened distal end annular stem surface or lower end annular stem surface 174 which is the distal annular base portion end of lower annular base portion 172 . or terminate to lower annular base portion end 176 . Base surface 171 and base portion 172 define a shortened distal end annular groove 178, as shown in FIG.

12 and 15, the lower annular base portion 172 further includes an inner cylindrical surface 175 that transitions upward to an inner annular shoulder stop surface 177, A spacer 160 is mounted over the inner annular shoulder stop surface 177 as shown in FIG. Additionally, the inner cylindrical surface 175 has an inner diameter that closely surrounds the lower cylindrical body member 120 of the central body member 102 at a location immediately above the annular shoulder stop surface 122 of the central body member 102 . and the annular shoulder stop surface 122 defines an axial stop for the lower annular base portion end 176 of the expandable mandrel collet 170 such that the expandable mandrel collet 170: It is adapted to be centrally mounted over and around elongated central body member 102 .

lower arm portion 186

As shown in FIG. 11, the lower arm portion 186 includes a distal or lower end portion 184 that is circumferentially spaced apart and has a lower annular It is attached to base portion 172 . As shown in FIG. 12, the lower arm portion 186 further includes an upper end portion that defines a middle arm portion that defines an internal annular concave shape. segmented surface or groove 191 and an outer radially outwardly extending annularly segmented stop disk portion 194 .

11 and 12, the segmented stop disk 194 comprises a plurality of circumferentially spaced upwardly extending collets defining an annular segmented stop disk. It surrounds the exterior of the middle arm portion of arm 180 and extends radially therefrom.

As shown in FIG. 12, each segmented stop disk 194 includes a proximally facing or upwardly facing stop disk surface 198 and a distally facing or downwardly facing stop disk surface 196 . Additionally, the plurality of lower arm portions 186 includes an inner cylindrical surface or interior segmented surface 188 that is dimensioned with an inner diameter that closely surrounds the spacer 160 surrounding the elongated central body member 102 . It is decided.

The distal or lower end of the inner segmented surface 188 merges radially inwardly into an inner annular shoulder stop surface 177, which has been described in detail above. provides a stop surface for the spacer 160 as described. The proximal or upper end of the inner segmented surface 188 merges into an inner annular concave segmented surface or groove 191 .

upper arm portion 190

11 and 12, a plurality of circumferentially-spaced upper arm portions 190 transition upwardly and radially outwardly from respective lower arm portions 186 to provide a plurality of , the plurality of free ends 199 being disposed above and radially outwardly from the lower arm portion 186 and the plurality of free ends 199 199 includes radially outwardly projecting segments defining segmented collars 200 each segment having an outer outwardly facing surface 202 . , and the outer, outwardly facing surface 202, in one embodiment, is outwardly rounded or arcuate in a shape corresponding to the arcuate groove of the exemplary embodiment of the pipette tip. ing.

Thus, upper arm portion 190 transitions upwardly and radially outwardly from segmented stop disk 194 to a plurality of radially outwardly projecting segments defining segmented collar 200 . , and the segmented collar 200 is configured to surround the central longitudinal axis 90 of the expandable mandrel collet coupling device 100, as illustrated in FIG.

Additionally, the plurality of circumferentially-spaced, radially outwardly and upwardly extending upper arm portions 190 each including a segment includes an inner surface 192, the inner surface 192 being: The proximally sloping annular side surfaces 216 of the annular wedge 210 (FIG. 7) form complementary sloped segmented inner surfaces, each decreasing distally with respect to the Z-axis. , the inner surface 192 of the upper arm portion 190 forms a radially outwardly and upwardly extending conically shaped gap 204 with respect to the elongate central body member 102 . (Fig. 15).

Specifically, and as shown in FIG. 15, the upwardly and radially outwardly sloping inner surfaces 192 of the plurality of circumferentially-spaced upper arm portions 190 are the upper arm portions. A distally tapered conical gap 204 is defined between the inner surface 192 of 190 and the combination of the lower portion of mounting flange 36 and the upper portion of spacer 160 . A tapered conical gap 204 is configured to receive a lower portion of an annular wedge 210 such that an annular wedge-shaped or sloped outer side surface 216 of the annular wedge 210 meets the segmented collar. A plurality of free ends 199 carrying protruding segments defining 200 are adapted to abut the inner surface 192 .

annular wedge 210

7, 13 and 15, annular wedge 210 includes a resilient wedge-shaped annular body portion having a circumferentially continuous generally wedge-shaped cross-section 211 . Annular wedge 210 includes a central inner annular surface 212 that defines a central annular opening 213 configured to movably surround body portion 102 . extending through an annular wedge 210 that is

Additionally, annular wedge 210 includes an upper planar circular surface 214 that is configured to make an electrical contact switch with LLD circuit ring end 366 of LLD circuit 364 . and extends radially outwardly from a central inner annular surface 212 to a surrounding outer edge surface 215 .

In addition, annular wedge 210 includes radially outwardly proximally sloping side surfaces 216 that extend from bottom annular end 218 to annular peripheral lip 218 . Extending radially upwardly and outwardly to the underside of 219 , an annular peripheral lip 219 extends radially outwardly and terminates on the surrounding outer edge surface 215 . there is Thus, the radially outward proximally sloping side surface 216 defines a distally tapered wedge surface 216 .

15, central annular opening 213 of annular wedge 210 is sized to allow passage of distal mounting flange 36 and elongated tubular upper shank member 108; It is adapted to allow seating abutment between the radially outwardly proximally sloping side surface 216 of the annular wedge 210 and the inner surface 192 of the plurality of radially outwardly projecting segments 200 . , distal axial translation of the annular wedge 210 results in radial projection of the radially outwardly projecting segments 200 of the expanding mandrel collet 170, and subsequent annular wedges. Proximal translation of 210 results in radial retraction of radially outwardly projecting segments 200 of expandable mandrel collet 170 .

As further illustrated in FIG. 15, shank member 108 of expandable mandrel collet coupling device 100 is configured to operably connect expandable mandrel collet coupling device 100 to pipette device 20 of pipette device assembly 10 . , configured to fit within the distal mounting flange 36 of the aspiration and dispensing cylinder 34 such that the longitudinal channel axis 80 and the central longitudinal axis 90 form coincident or common axes. ing.

As the annular wedge 210 moves axially downward under the force provided by the squeeze sleeve 46, the expanding collet 170 moves around the first perimeter as generally illustrated in FIG. from an unexpanded state with is further configured to allow

Operation of the squeeze motor

15, the expandable mandrel collet coupling device 100 is shown with the upper planar circular surface 214 of the annular wedge 210 disposed adjacent the distal end 50 of the squeeze sleeve 46. , is configured to fit within the distal mounting flange 36 . 4 and 15, actuation of squeeze motor 52 in a first direction results in linear axial translation of squeeze sleeve 46 in a distal or vertically downward direction, resulting in an annular A force is adapted to axially apply to upper surface 214 of wedge 210 , which causes distally tapered wedge surface 216 to slide axially down and further into conical gap 204 . resulting in pressing a distally tapered wedge surface 216 against the inner surface 192 of the plurality of radially outwardly projecting segments 200 and the outer radially outward surface 202 of the segment 200 . (FIG. 11) is pushed radially outward against the spring tension of the upwardly extending collet arm 180 and the disposable pipette tip 220 as illustrated in FIG. 29 described below. is adapted to be pressed into contact with the first working surface of the pipette tip in the form of surface 244 defining groove 246 of the pipette tip.

Subsequent actuation of squeeze motor 52 in a second direction opposite the first direction returns distal end 50 of squeeze sleeve 46 to the home position shown in FIG. As a result of the release of stored potential energy within the collet arm 180, the annular wedge member 210 slides axially upward, thereby projecting a plurality of radially outwardly. The result is that the outer radially outward facing surface 202 of segment 200 is recessed from groove 246 of disposable pipette tip 220 .

pipette tip 220

As illustrated in FIGS. 2 and 16 and as described above, the expandable mandrel collet coupling device 100 is mounted between the disposable pipette tip 220 and the pipette device 20 of the pipette device assembly 10. to provide an open communication coupling.

16-18, and in one exemplary embodiment, a disposable pipette tip 220 includes an elongate tubular pipette tip body 222 having a central longitudinal axis 224. As shown in FIG. Pipette tip body portion 222 includes an elongated surrounding sidewall 226 that extends along a central longitudinal axis 224 with a proximal or upper annular end face 228 and a distal or lower annular end face. 230, which define surrounding open proximal annular end 232 and distal annular end 234, respectively. Elongated enclosing sidewall portion 226 includes an interior surface 236 that defines a pipette tip passage opening 238 extending along central longitudinal axis 224 of pipette tip body portion 222 . , extending longitudinally between an open upper annular end 232 and an open lower annular end 234 .

Thus, pipette tip passage opening 238 allows central channel 136 of expandable mandrel collet coupling device 100 ( FIG. 16 ) to pass through when coupling device 100 is coupled between pipette device 20 and pipette tip 220 . Via, from an area outside of open distal annular end 234 (FIG. 18) through pipette tip 220 provides open communication to pipette device channel 40 (FIG. 15). In this coupling configuration, central longitudinal axis 224 of pipette tip body 222 is coextensive with longitudinal channel axis 80 of pipette device 20 .

In an alternative embodiment, pipette tip passage opening 238 is aligned with central channel 3136 of nozzle 3102 and pipette tip 220 when nozzle 3102 and leaf spring coupling device 3100 are coupled between pipette device 3020 and pipette tip 220 . Open communication from an area outside the open distal annular end 234 (FIG. 18) via the leaf spring coupling device 3100 (FIG. 80) through the pipette tip 220 to the pipette device channel 3040 (FIG. 79). I will provide a. In this coupling configuration, central longitudinal axis 224 (FIG. 17) of pipette tip body 222 is coextensive with longitudinal channel axis 3080 of pipette device 3020 .

first inner surface section

18, and in one exemplary embodiment, the interior surface 236 of the elongated enclosing sidewall 226 includes a top annular chamfered interior surface 240 and a top annular chamfered interior surface 240. 240 extends radially inward distally from proximal annular end face 228 of pipette tip 220 and transitions to a first substantially cylindrical inner surface section 242 having a first diameter. is terminated by

An axially arcuate circumferential surface defining a groove

As shown in FIG. 18, and in one exemplary embodiment, the first substantially cylindrical inner surface section 242 has an elongated surrounding sidewall defining a circumferential annular groove 246. It includes an axially arcuate circumferential inner surface 244 formed into portion 226 . The annular groove 246 defines a first substantially cylindrical inner surface section 242 with an upper first substantially cylindrical inner surface portion and a lower first substantially cylindrical inner surface portion of substantially equal diameter. It is divided into cylindrical inner surface portions. Annular groove 246 thus defines a circumferentially radially outwardly extending concave shaped inner surface of first substantially cylindrically shaped inner surface section 242 with an arcuate surface longitudinal cross-section. Provide interruptions. The arcuate circumferential inner surface 244 is also configured with alternative surface cross-sections, as discussed below. And, in one embodiment, the first substantially cylindrical inner surface section 242 lacks an arcuate circumferential inner surface 244 that defines a circumferential annular groove 246 .

18 and 19, the axially arcuate inner circumferential surface 244 that defines the annular groove 246 includes an upper annular transition edge 248, the upper annular transition edge 248 extending axially The inner arcuate circumferential surface 244 transitions distally into an upper axially arcuate circumferential surface sector portion 250 . Subsequently, the upper axially arcuate circumferential surface sector portion 250 is followed by the lower axially arcuate circumferential surface sector portion 244 of the axially arcuate circumferential surface 244 . It transitions distally at 252. A lower axially arcuate circumferential surface sector portion 252 then terminates into a lower annular transition edge 254 .

An upper axially arcuate circumferential surface sector portion or upper portion 250 extends from the upper annular transition edge 248 to a central longitudinal axis 224 that defines the circumferential annular center of the annular groove 246 . An annular groove 246 is provided having a radius that increases with respect to the central longitudinal axis 224 (FIG. 17) of the pipette tip 220 to the maximum radius of the groove 246 . A lower axially arcuate circumferential surface sector portion or lower portion 252 extends from the maximum radius defining the circumferential annular center of the annular groove 246 to the lower annular transition edge 254 . An annular groove 246 is provided having a decreasing radius with respect to the central longitudinal axis 224 of tip 220 .

Second inner surface section and annular shoulder stop surface

As shown in FIG. 18, the first substantially cylindrical inner surface section 242 is advanced axially distally by the second substantially cylindrical inner surface section 262, and The second substantially cylindrical inner surface section 262 has a second diameter that is less than the first diameter of the first substantially cylindrical inner surface section 242, and the first substantially cylindrical inner surface section 262 has a a proximally facing radially inwardly extending annular shoulder seating surface interposed between the generally cylindrical inner surface section 242 and a second substantially cylindrical inner surface section 262; or to form an axial stop surface 260 .

In one exemplary embodiment, the proximally-facing axial stop surface 260 is substantially planar and extends along the central longitudinal axis of the pipette tip body 222, as illustrated in FIG. , generally perpendicular to H.224.

Third inner surface section and sealing sheet

18 and 19, the second substantially cylindrical inner surface section 262 is axially distally offset by a third substantially cylindrical inner surface section 272. The advanced, third substantially cylindrical inner surface section 272 has a third diameter less than the second diameter of section 262 .

A frusto-conical annular sealing seat or stop surface 270 is interposed between the second section 262 and the third section 272, the frusto-conical annular sealing seat or stop surface 270 being , defines a circumferentially radially inwardly angled and distally extending distal work surface 270 . The frusto-conically shaped annular sealing seat surface 270 includes an upper annular sealing seat edge 266 which has a second substantially cylindrical inner surface section 262 and a frusto-conical shape. defines an annular boundary with the annular sealing seat surface 270 of the . In addition, the frusto-conical annular sealing seat surface 270 includes a lower annular sealing seat edge 268 , which is in contact with the frusto-conical annular sealing seat surface 270 . The diameter of the upper annular sealing seat edge 266 is greater than the diameter of the lower annular sealing seat edge 268 .

Thus, the frusto-conical annular sealing seat surface 270 defines a second circumferentially radially inwardly angled and distally extending working surface or sealing seat surface 270; It is interposed between a second substantially cylindrical inner surface section 262 and a third substantially cylindrical inner surface section 272 .

As shown, the sealing sheet surface 270 is disposed at an acute angle with respect to the central longitudinal axis 224, an acute angle creating a sharp sealing sheet surface angle with respect to the central longitudinal axis 224 (FIG. 17). demarcated. In one embodiment, a preferred sharp sealing sheet surface angle with respect to the central longitudinal axis 224 is from about 15 degrees to about 35 degrees, with a preferred angle of about 25 degrees. As illustrated in FIG. 41, the acute sealing sheet surface angle of alternative sealing sheet surface 2270 with respect to central longitudinal axis 224 is about 90 degrees.

lower inner surface

FIG. 18 shows a fourth inner surface section 274 continuing a third substantially cylindrical inner surface section 272 and a fifth inner surface section 275 distal to the fourth inner surface section 274 . It further illustrates that it follows .

In one exemplary embodiment, the fourth inner surface section 274 tapers distally or tapers to the distal annular end 276 of the third substantially cylindrical inner surface section 272 . , to the proximal annular end 278 of the fifth inner surface section 275 . And, the fifth inner surface section 275 tapers distally or, from the proximal annular end 278 of the fifth inner surface section 275, the opening of the pipette tip 220 intended for immersion. The diameter decreases to a rounded distal annular end 234 . Additionally, and in one exemplary embodiment, fifth inner surface section 275 has a greater taper than fourth inner surface section 274 .

External longitudinal rib

Referring to FIG. 17, one exemplary embodiment of a pipette tip 220 includes a plurality of circumferentially-spaced, longitudinally-extending external ribs 280 that define a proximal annular end surface. 228 is disposed over the tubular pipette tip body portion 222 adjacent the perimeter of 228, and from there a third substantially cylindrical inner surface section, as shown in FIG. It extends longitudinally outward to the exterior area of surrounding sidewall 226 adjacent 272 .

In one exemplary embodiment, a plurality of circumferentially-spaced, longitudinally-extending external ribs 280 provide support for pipette tip 220 on or in support surface 282 . The pipette body 222 passes through the support surface 282 , eg, via support surface aperture openings 284 . One exemplary embodiment of support surface 282 is, but is not limited to, in the form of a labware in the form of a tip rack, as known in the art and as notified by this disclosure. could be.

Automated pipetting workstation or system

5 and 20, and in one example of use and operation, one or more of pipetting device assemblies 10 are employed in an automated pipetting workstation or system 300 to automate A modified pipetting workstation or system 300 generally provides, but is not limited to, programmed transfer of liquids between containers, such as for performing programmed transfers of liquids between containers. 2 includes the process of attaching and ejecting one or more disposable pipette tips 220 to the expandable mandrel collet coupling device 100 operably carried by the pipette device 20 .

In one exemplary embodiment, the automated pipetting workstation 300 generally includes a robotic gantry 302 vertically mounted on a horizontally disposed workstation deck 304. Above it carries at least one pipetting device assembly 10 . Pipette device assembly 10 can include a single-channel pipetting head or a multi-channel pipetting head.

Additionally, the robotic gantry 302 typically provides two or three degrees of freedom, the three degrees of freedom being longitudinal translation along an axis defining the X-axis and longitudinal translation along the Y-axis. including latitudinal translation along the defining axis and vertical (up and down) translation along the axis defining the Z-axis, the pipette device assembly 10 measuring the length (X-axis) and width of the deck. (Y-axis) and vertically to it up and down (Z-axis). Two degrees of freedom typically provide the robotic gantry with the ability to translate the pipetting device assembly 10 vertically and either longitudinally or laterally.

In one exemplary embodiment, the automated pipetting workstation 300 further includes a main controller 306, a pipette axis controller 308, and a power supply 310, the power supply 310 connecting the main controller 306, the pipette axis controller 308, and provide power to the pipette device assembly 10

Additionally, and in one exemplary embodiment, a computer/controller 320 is used in conjunction with the workstation 300 and, for example, for disposable pipette tip 220 attachment and ejection (coupling and ejection) detailed below. It is also possible to communicate with the main controller 306 and the pipette axis controller 308 to control the robotic gantry 302 and the pipette device assembly 10, including associated process protocols of the pipette device assembly 10, such as the release process.

In one exemplary embodiment, computer/controller 320 typically includes a processor device or central processing unit (CPU) 322, a hardware read-only memory device (ROM) 324, and a hardware main memory device (RAM). ) 326 and a non-transitory computer readable medium or memory 330 having an operating system 332 and software 334 (e.g., a user-defined process 336 for the pipette device assembly 10 stored thereby). 328 , a user display 338 , a user input device 340 , an input interface 342 , an output interface 344 , a communications interface device 346 , and a system bus 348 , which is between the devices of the computer/controller 320 . includes one or more conductors or communication paths that allow communication of Computer/controller 320 may also be operably coupled to LAN and/or server 350 . Power supply 352 provides power for computer/controller 320 .

The example automated pipetting workstation 300 depicted above, including software, is currently manufactured and sold by the Hamilton Company of 4970 Reno Energy Way, Nevada, USA, the assignee of the present patent application.

Pipette tip pick-up process with expandable mandrel collet coupling device

FIGS. 21-31 illustrate details of an exemplary embodiment of successive stages of a pipette tip pick-up process, including, among other things, an expanding mandrel collet coupling device operably carried by pipette device 20. 100 illustrates how the pipette tip 220 attachment is secured. As noted above, and in one exemplary embodiment, pipette tip 220 may be supported by support surface 282 .

As shown in FIG. 21, the expandable mandrel collet coupling device 100 is connected to the pipette device 20 and upon command, the coupling device 100 opens the open proximal end of the pipette tip 220. 232 and each of their respective central longitudinal axes are aligned along the Z-axis. Eject sleeve 62 is in the ejected position, squeeze sleeve 46 is in the uncompressed position, expandable mandrel collet 170 is relaxed, and distal O-ring 140 is uncompressed.

Next, FIG. 22 illustrates the expanding mandrel collet coupling device 100 being moved down the Z-axis into the pipette tip 220, and the distal elastomer carrying portion of the coupling device 100 is removed. portion is lowered and threaded into the inner cylindrical proximal end portion of the pipette tip 220 while maintaining the distal O-ring 140 in an uncompressed state and the upwardly facing annular shoulder of the pipette tip 220 seats or Distal O-ring 140 is brought into contact with annular sealing seat or stop surface 270 of tip 220 before stop surface 260 and downward axial stop disk surface 196 of stop disk 194 are mated. there is

FIG. 23 then illustrates the coupling device 100 being moved further down along the Z axis. Additionally, and with reference to FIGS. 23-25, the squeeze sleeve 46 is moved down along the Z-axis, pushing against the LLD circuit ring end 366, the LLD circuit ring end 366 forming an annular shape. 25 while maintaining the plurality of radially outwardly projecting segments 200 in an unexpanded state as detailed in FIG. while maintaining the distal O-ring 140 in an uncompressed state and before the stop surface 260 of the pipette tip 220 and the axial stop shoulder surface 196 of the stop disk 194 are mated, as detailed in 23, over the expandable mandrel collet 170 and between the stop surface 260 of the pipette tip 220 and the axial stop shoulder surface 196 of the stop disk 194 of the expandable mandrel collet 170, as detailed in FIG. , the gap 298 is maintained.

Next, FIG. 26 illustrates the squeeze sleeve 46 being moved further down along the Z-axis, creating an annular groove on the inner surface 192 of the plurality of radially outwardly projecting segments 200 . The outer radially outward rounding of the plurality of radially outwardly projecting segments 200 is adapted to compress the wedges 210 and push them radially outwardly, as detailed in FIG. The ridged surface 202 is adapted to abut against an axially arcuate circumferential surface sector portion 250 on the upper side of the groove 246 of the disposable pipette tip 220 and has a plurality of radially outwardly projecting portions 250 . The process of squeezing or pushing the segment 200 into the groove 246 and, first, an axially arcuate circle above the axially arcuate circumferential inner surface 244 that defines the groove 246 . It is adapted to initiate the process of squeezing or pushing into contact with the circumferential surface sector portion 250 . 26, the action of the plurality of radially outwardly projecting segments 200 extending or projecting into groove 246 as detailed in FIG. An axially upward force is produced which initiates the process of pulling up the pipette tip 220 and causing the annular shoulder seating surface 260 of the pipette tip 220 to seat against the axial stop shoulder surface 196 of the stop disk 194 . 23 to close gap 298 (FIG. 23) and compress distal O-ring 140 by sealing seat or stop surface 270 of tip 220, as detailed in FIG. It's like

FIG. 29 shows that the squeeze sleeve 46 is moved down the Z-axis by the configured predetermined length until the squeeze sleeve 46 is locked in place and the annular wedge 210 is pulled by the squeeze sleeve 46. It is shown to result in being stopped and locked in place.

As a result, the plurality of radially outwardly projecting segments 200 are radially extended a desired distance or value, as illustrated in FIG. The axial stop shoulder surface 196 is adapted to fully seat against the annular shoulder seating surface 260 of the pipette tip 220 such that the seating of the two surfaces 196, 260 is substantially perpendicular to the Z axis. It is oriented along the X axis so as to form an orthogonal datum between the two axes.

At the same time, distal O-ring 140 is compressed to a desired distance or value, as illustrated in FIG. , the cross-section of which is adapted to its final compressed non-circular configuration whereby the expandable mandrel collet coupling device 100 and pipette are operably carried by the pipette device 20. Complete the connection that secures the tip 220 attachment.

Upon completion of the attachment securing process detailed above, the plurality of radially outwardly projecting segments 200 and distal elastomeric element 140 work in combination to provide a fluid-tight seal. A plurality of radially outwardly projecting segments 200 that make up the coupling are at least partially received within the circumferential groove 246 and also have a circumference that defines the circumferential groove 246 . Seated at least partially on an arcuate interior surface 244 (FIG. 18) of direction, distal elastomeric element 140 seals against surface 270 of pipette tip 220, and in one embodiment , surface 270 provides a radially inwardly angled and distally or downwardly extending surface.

Thus, the plurality of radially outwardly projecting segments 200 move radially outwardly to engage circumferential grooves 246 (Fig. 18) and interlock with tips 220, and radially inwardly. oriented to release tip 220 as a function of movement of annular wedge 210 . Applying a force to move the annular wedge 210 axially downward results in the plurality of radially outwardly projecting segments 200 being urged to a radially outward position, Releasing the force on the annular wedge 210 results in the release of energy from the cantilevered arms 180 (Fig. 11) supporting the plurality of radially outwardly projecting segments 200, the segments being , to bounce from a radially outward position to a radially inward position.

Disposable pipette tip ejection process

FIGS. 21-31, in turn, detail sequential steps of an exemplary method or process for ejecting a pipette tip 220 from an expandable mandrel collet coupling device 100 operatively carried by a pipette device 20. Illustrated. This tip ejection process sequence is similar to the attachment or tip pick-up fixation process sequence except in reverse, FIG. As shown, it provides force to help remove tip 220 during the ejection process.

In one exemplary embodiment, the ejection process consists of (1) positioning the tip where the tip is to be discarded (e.g., a waste container, etc.); so that the force is released from the annular wedge 210 and consequently the force is also released from the plurality of radially outwardly projecting segments 200 and from the grooves 246 in the tip 220. , and distal O-ring 140 begins to release stored elastic potential or spring energy as a force against tip 220, resulting in a spring-loaded ejection. The sleeve 62 also pushes the tip 220 and pushes it off so that the tip begins to release from the plurality of radially outwardly projecting segments 200; and (3) an upward squeeze; Continuing the movement of sleeve 46, the plurality of radially outwardly projecting segments 200 continue to retract from groove 246 in tip 220, distal O-ring 140 and spring loaded eject sleeve. 62 pushes the tip 220 and pushes it off, the tip 220 continuing to release from the plurality of radially outwardly projecting segments 200; and (4) squeezing the sleeve 46 to its uppermost position. In the further continuation of the movement, the plurality of radially outwardly projecting segments 200 return to their original retracted free state, completely free of grooves 246 in tip 220. , the distal O-ring 140 returns to its original shape and the spring-loaded ejection sleeve 62 pushes against the tip 220 until the tip is pushed out of the coupler 100 by the spring-loaded ejection sleeve 62, causing the spring to The loadable eject sleeve 62 includes a step that becomes fully extended.

In light of the foregoing, those skilled in the art will recognize that these tip attachment and ejection processes are applicable to a wide variety of mechanically and/or automatically driven pipette types and designs. becomes.

Coupling force and ejection force

FIG. 32 illustrates a schematic vector diagram of a plurality of radially outwardly projecting segments 200 of expandable mandrel collet coupling device 100 initially extending into groove 246, with a plurality of The radially rounded surface 202 of the radially outwardly projecting segment 200 of the is in contact with the upper corner of the tip groove above the center of the segment radius, and holds the pipette tip 220. An axially upward force that pulls upward results. As illustrated in FIG. 32, the segment force (Fsegment_resultant) for each of the plurality of radially outwardly projecting segments 200 is composed of two components: an axial force (Fsegment_axial) component and a radial force (Fsegment_axial) component. directional force (Fsegment_radial component).

A plurality of radially outwardly projecting segments 200 above the center of the segment radius (dimension Z in FIG. 33) contact the upper corners of the tip grooves. Fsegment_axial increases as the distance between the corners of the groove increases. Thus, at the beginning of the chip pick-up process, the segment axial force (Fsegment_axial) starts low, as shown in FIG. 32 and in detail in FIG. As noted, it increases to its maximum at the end of the chip pick-up process.

Referring to FIG. 33, the ratio of Z/R is equal to SIN(ω), and SIN(ω) is equal to (Fsegment_axial)/(Fsegment_resultant). As a result, (Fsegment_axial) is equal to (Fsegment_resultant) multiplied by the ratio of Z/R. From this, the result is that (Fsegment_axial) increases as Z increases.

Referring to FIG. 34, the segment axial force (Fsegment_axial) seats the stop disk 194 against the seat 260 of the tip 220 and provides the force required to overcome the O-ring axial force (Fdistal_ring_axial). , compressing the distal O-ring 140 . O-ring 140 has an O-ring force (Fdistal_ring_resultant) resulting from being compressed, which has two components, an axial component (Fdistal_ring_axial) and a radial component (Fdistal_ring_radial). )including. Additionally, the segment radial force (Fsegment_radial) provides the radial force required to lock the segment into the tip groove 246 (FIG. 18) and the distal O-ring radial force component (Fdistal_ring_radial ) provides the radial force required to maintain the seal against the tip. Additionally, the segment-to-tip groove geometry, which causes Fsegment_axial to increase (increase dimension Z) as the segment enters the groove, helps overcome the O-ring axial force (Fdistal_ring_axial) component. , the distal O-ring 140 can be fully compressed to the desired degree. Additionally, a distal O-ring axial force (Fdistal_ring_axial) component provides force to help remove tip 220 during the ejection process.

Alignment/Misalignment

Axial shoulder surface 196 of coupler 100 and axial shoulder seat 260 of tip 220 are critical for proper tip alignment. Accordingly, coupler 100 and chip 220 are configured such that the plurality of radially outwardly projecting segments 200 push axial shoulder surface 196 and axial shoulder seat 260 together to prevent misalignment. It is This is because misalignment errors (E) can be significant if the shoulders are not properly fitted, especially if they are tilted.

For example, as illustrated in FIGS. 35 and 36, the relationship between misalignment angle (Φ), chip axial distance (D), and position error (E) is E=D*TAN(Φ) is. For example, with a misalignment angle (Φ) of 2 degrees and a chip axial distance of 90 millimeters, the position error (E) is 3.14 millimeters. This is considered very high given that typical positional error tolerances are typically plus or minus 0.5 millimeters.

FIG. 37 illustrates correct chip alignment, with the axial shoulder surface 196 and the axial shoulder seat 260 in coplanar contact with each other to provide proper alignment, Maintaining a constant tip axial distance D from tip seat 260 to distal end 230, along vertical or axial axis Z and vertical axis X, pipette tip end 230 has a known and controlled distance. This is important to allow the pipette device to target small bores and small volumes of liquid. Additionally, a smaller volume of liquid, resulting from a known fixed distance of the pipette tip, can be transferred to work surface 290 (onto or from which liquid 292 will be transferred). of controlled pipette tip/liquid contact.

Dimensions and relationships

Therefore, for proper use and operation, the dimensions between coupler 100 and tip 220 are related accordingly.

15, 38 and 39, tip groove diameter A is sufficient to allow segment 200 to pull tip 220 up and properly lock tip 220 in place. It must be big. Conversely, if it is too large, segment 200 may not be pushed in enough to obtain a good lock. Additionally, inner diameters B and C must be greater than outer diameter K of stop disk 194 and outer diameter L of annular base 172, respectively. However, they should not be too large. The reason is that this can result in a poor fit and/or misalignment.

38 and 39, the dimension S from the tip seat to the groove must be matched to the dimension M from the stop disc seat surface 196 to the segment 200 center. This relationship is important for the connection between tip 220 and stop disk 194 .

19, 38 and 39, the dimension from the tip seat surface 260 in FIG. 19 to the O-ring seal land 266 (dimension F in FIG. vertical lip surface 171 (dimension N in FIG. 39). These dimensions control the amount that the distal O-ring 140 is compressed and therefore how well it seals. The tip surface 260 and stop disk seating/coupling surface 196 must mate perfectly to provide proper alignment and to maintain the tip axial distance D. FIG.

Referring to FIGS. 37-39, the dimension D (or axial distance) from the tip seat 260 to the distal end 230, along with the fit of the coupling seat, is a known and controlled dimension of the pipette tip end. Establish distance. This is important to allow the pipette device to target small bores and small volumes of liquid. Additionally, smaller volumes of liquid can be transferred, resulting from a known fixed distance of the pipette tip, and the pipette to the work surface onto which or from which the liquid will be transferred. Allows controlled tip/liquid contact.

15, 38 and 39, tip internal diameter G is smaller than diameter L of base 172 to create a seat or land against which distal O-ring 140 seals. must be If the diameter G is too large, the distal O-ring may not seal well. If the diameter is too small, the distal O-ring 140 may not fully compress, which may prevent the stop disk 194 from seating or may cause damage to the distal O-ring 140. may occur. Additionally, along with diameter G, ramp length H controls the seat or land that mates with O-ring 140 . These dimensions are important in providing a good O-ring seal. If the ramp length H is too long, the O-ring may not seal well. If H is too short, the O-ring may not fully compress and may prevent stop disk 194 from seating or may cause harm to O-ring 140. be.

Liquid level detection (LLD) circuit contacts

Referring to FIG. 40, and in one exemplary embodiment, pipette device assembly 10 further includes a liquid level detection circuit assembly. The liquid level detection circuit assembly includes a liquid level detection or LLD circuit board 360, which includes processing circuitry 362 electrically coupled to LLD circuit contacts 364, which are: Operably connected to squeeze sleeve 46, which is made of an electrically non-conductive material so that it is insulated from the rest of the assembly, contact 364 is Terminating in a circuit contact ring end 366 embedded in the bottom area of the squeeze sleeve 46, the squeeze sleeve 46 can be positioned in a non-contacting state illustrated in FIG. 22 and a contacting state illustrated in FIG. (and thus in contact with a plurality of conductive segments or elements that couple with the first work surface inside conductive tip 220), the circuit contact ring end 366 is positioned between the annular wedge 210. configured for selective contact with the

29, the LLD circuit contact 364 includes a ring end 366 captured between the squeeze sleeve 46 and the annular wedge 210 so that electrical closure or contact is made with the LLD circuit board. 360 (FIG. 40) between processing circuitry 362 and an annular wedge 210 made of a conductive material. An annular wedge 210 pushes against a plurality of radially outwardly projecting segments 200 made using an electrically conductive non-compliant material to make electrical contact therewith.

Thus, with the tip attached and with the plurality of radially outwardly projecting segments 200 squeezed or pushed to lock into the tip groove 246 of the tip 220, the plurality of Radially outwardly projecting segments 200 make electrical contact with tip 220, which is also made of an electrically conductive material. As a result, and with reference to FIG. 40, this completes the circuit between the processing circuitry 362 of the LLD circuit board 360 and the chip 220 .

Additionally, the stop disk mounting post or distal mounting flange 36 is formed from a non-conductive material. Thus, the body member 102 and the plurality of radially outwardly projecting segments 200 are insulated from the rest of the assembly.

Moreover, the processing circuitry 362 of the LLD circuit board 360 detects signal changes when the tip 220 contacts the liquid, thereby detecting the surface of the liquid being transferred or the liquid being transferred onto or from it. It has the ability to detect surfaces that Again, when the coupling device 100 is attached to the tip 220 and the plurality of radially outwardly projecting segments 200 are pushed radially and circumferentially, the tip of the tip 220 is pushed. Actuation occurs when locked into the groove.

Alternate Exemplary Embodiment

FIG. 41 is positioned over an exemplary embodiment of a disposable pipette tip 220 that includes an alternative sealing seat surface 2270 having an angle of substantially 90 degrees with respect to the central longitudinal Z-axis of the pipette tip 220. 1 illustrates an exemplary embodiment of an expandable mandrel collet coupling device 100 with.

FIG. 42 illustrates an exemplary embodiment of the expandable mandrel collet coupling device 100 positioned within a disposable pipette tip that includes an alternative sealing sheet surface 2270, the tip 220 being its final has been lifted to a fully seated condition, annular wedge 210 has been moved to its final position to define a final coupling condition, and distal elastomeric element 140 has replaced It is in a final compressed and seated sealing condition against the static sealing seat surface 2270 .

43 details the final compression of distal elastomeric element 140 against alternative sealing seat surface 2270. FIG.

FIG. 44 illustrates the upper interior of disposable pipette tip 220 including another alternative sealing seat surface in the form of circumferentially radially concave sealing seat surface 3270 . FIG. 45 details the circumferential radially concave sealing seat surface 3270 illustrated in FIG.

FIG. 46 illustrates an exemplary embodiment of a disposable pipette tip 220 illustrating further alternative sealing seat surface details in the form of a circumferential radially convex sealing seat surface 4270 . FIG. 47 details the circumferential radially convex sealing seat surface 4270 illustrated in FIG.

FIG. 48 illustrates an exemplary embodiment of a disposable pipette tip 220 illustrating yet a further alternative sealing seat surface in the form of a circumferentially upward tooth edge sealing seat surface 5270 . FIG. 49 details the circumferentially upward facing tooth edge sealing sheet surface illustrated in FIG.

FIG. 50 shows V-shaped circumferential inner surface 2244 of disposable pipette tip 220 that opens toward the longitudinal Z-axis and has a V-shaped cross-section as shown. Fragment of an exemplary embodiment of an expandable mandrel collet coupling device 100 positioned above an exemplary embodiment of a disposable pipette tip 220 including an alternative V-shaped groove 2246 defined by 1 is a side view of a longitudinal section; FIG.

FIG. 51 illustrates the expandable mandrel collet coupling device 100 positioned in a disposable pipette tip 220 that includes an alternative V-shaped groove 2246 (FIG. 50), the tip 220 having its final 2246, with the rounded surfaces 202 of the plurality of expandable mandrel collet segments 200 extending into the V-shaped grooves 2246 to form a V-shaped groove 2246. Abutting the inner circumferential surface, the distal elastomeric element 140 is in a final compressed and seated sealing condition against the sealing seat surface 270 of the tip.

FIG. 52 shows the rounded surface 202 of one of the expanding mandrel collet segments 200 extending into the V-shaped groove 2246 to define the V-shaped groove 2246. It is shown abutting the inner circumferential surface 2244 of the V-shape.

FIG. 53 is an expanded view positioned above a second exemplary embodiment of a disposable pipette tip 1220 lacking an arcuate circumferential inner surface 244 defining a circumferential annular groove 246. FIG. 1 illustrates an exemplary embodiment of a mandrel collet coupling device;

FIG. 54 details the interior of a second exemplary embodiment of a disposable pipette tip 1220, which interrupts the first substantially cylindrical interior surface section 242 illustrated in FIG. The smoothed interior surface section 242 lacks interruptions thereby defining an uninterrupted interior surface section 1242 of the disposable pipette tip 1220, the interior surface section 1242 defining a first work surface. are similar in all respects, except that

FIG. 55 illustrates an exemplary embodiment of the expandable mandrel collet coupling device 100 positioned within the second exemplary embodiment of the disposable pipette tip 1220, the stop of the coupling device 100 The disk shoulder surface 196 abuts the axial stop surface 260 of the second exemplary embodiment of the disposable pipette tip 1220, and the rounded surfaces 202 of the plurality of expandable mandrel collet segments 200 are disposable. Extending against the inner surface 1242 of the surrounding sidewall of the second exemplary embodiment of the pipette tip 1220, resulting in a deformation 1244 of the inner surface 1242, the distal elastomeric element 140 is disposable. The second exemplary embodiment of the pipette tip 1220 is in its final compressed and seated sealing condition against the sealing seat surface 270 .

FIG. 56 illustrates that the rounded surface 202 of one of the plurality of expandable mandrel collet segments 200 of the expandable mandrel collet coupling device 100 may be rounded to the second end of the disposable pipette tip as shown in FIG. extends against the inner surface 1242 of the surrounding sidewall portion of the exemplary embodiment of and details deforming 1244 it.

57-67 show alternative groove shapes for the circumferential annular tip groove for at least the segment 200 shown in FIG. 19 and at least the V-shaped groove segment 200 shown in FIG. FIG. 12 is a fragmentary longitudinal cross-sectional side view of an exemplary embodiment of a disposable pipette tip containing embodiment;

In particular, FIGS. 57-67 illustrate alternative groove configurations 2251-2261, respectively, for receiving segment 200. FIG.

Alternate Exemplary Embodiment Collet 2170

FIG. 68 is a second or alternative to expandable mandrel collet 2170 configured as a direct alternative to expandable mandrel collet 170 (FIG. 5) of expandable mandrel collet coupling device 100 (FIG. 5). 1 illustrates an exemplary embodiment. Expanding mandrel collet 2170 is similar in function to collet 170, but is configured for improved performance and service life.

68 and 69, the expanding mandrel collet 2170 includes a plurality of circumferentially spaced upwardly extending collet arms 2180 that extend radially outwardly. , arcuately transitioning upwardly from the lower annular base portion 2172 and terminating in a segmented free end 2200 disposed axially above the lower annular base portion 2172 . defines a segmented color. The plurality of circumferentially spaced upwardly extending collet arms 2180 are one of the plurality of circumferentially spaced upwardly extending kerfs or slots 2182 . are separated from each other by

68 and 69, each of the plurality of upwardly extending collet arms 2180 includes a respective lower arm portion 2186 that transitions to a respective upper arm portion 2190. are doing. In one embodiment, a plurality of circumferentially-spaced lower arm portions 2186 form a surrounding lower body portion 2181 and comprise a plurality of circumferentially-spaced arms. An upper arm portion 2190 arranged in a truncated cone forms a surrounding frusto-conically shaped upper body portion 2183 that extends radially outwardly and upwardly from the lower body portion 2181. are migrating.

68 and 69, distal or lower annular base portion 2172 includes a distally or downwardly facing base surface 2171 . Distal facing base surface 2171 merges downward into a shortened distal or lower end annular stem surface 2174 that aligns with the distal annular base portion end or underside of lower annular base portion 2172 . Terminates to annular base portion end 2176 . Base surface 2171 and lower end annular stem surface 2174 define a shortened distal end annular groove.

As shown in FIG. 69, the lower annular base portion 2172 further includes an inner cylindrical surface 2175 that transitions upward to an inner annular shoulder stop surface 2177. .

As further illustrated in FIG. 69, the lower arm portion 2186 includes circumferentially spaced lower end portions 2184 that are attached to the lower annular base portion 2172 . there is Lower arm portion 2186 further includes an upper end portion that defines a middle arm portion with an inner annular concave segmented surface or groove 2191 . , and an outer radially outwardly extending annularly segmented stop disk portion 2194 . The segmented stop disk 2194 extends over the exterior of the middle arm portion of the plurality of circumferentially spaced upwardly extending collet arms 2180 defining an annular segmented stop disk. surrounds and extends radially therefrom. Each segmented stop disk 2194 includes a proximally facing or upwardly facing stop disk surface 2198 and a distally facing or downwardly facing stop disk surface 2196 . Additionally, the plurality of lower arm portions 2186 includes an inner cylindrical surface or internal segmented surface 2188 that surrounds the elongate central body member 102 (FIG. 10) with spacers 160 (FIG. 10). It is sized with a closely surrounding inner diameter.

68 and 69, a plurality of circumferentially-spaced upper arm portions 2190 transition upwardly and radially outwardly from respective lower arm portions 2186 to provide a plurality of , the plurality of free ends 2199 being disposed above the lower arm portion 2186 radially outward from the lower arm portion 2186 and the plurality of free ends 2199 2199 includes radially outwardly projecting segments defining a segmented collar 2200 , each segment having an outer outwardly facing surface 2202 . , and the outer, outward facing surface 2202 is, in one embodiment, outwardly rounded or arcuate in shape. Thus, upper arm portion 2190 transitions upwardly and radially outwardly from segmented stop disk 2194 to a plurality of radially outwardly projecting segments defining segmented collar 2200 . are doing. Additionally, the plurality of circumferentially-spaced, radially outwardly and upwardly extending upper arm portions 2190 each including a segment includes an inner surface 2192, the inner surface 2192 being: It forms a sloped segmented inner surface complementary to the proximally sloped annular side surface 216 (FIG. 13) of the annular wedge 210 (FIG. 13).

Comparing FIGS. 12 and 69, the expanding mandrel collet 2170 widens the respective bases of the respective ends 2184 of the respective arms 2180 relative to the expanding mandrel collet 170, and the expanding mandrel collet With respect to 170 , ends 2184 of arms 2180 extend radially outward to increase the radius of each of arms 2180 . Pushing the lower ends outward and increasing their diameter allows for a larger chord segment or width at the bottom end 2184 of each of the extending arms 2180, Increasing the width of each of the extending arms 2180 improves the strength of each of the extending arms 2180. Additionally, increasing the radial extension at the lower ends 2184 of the arms 2180 provides increased strength. The linking function does not change.

From an engineering perspective, the extending arm 2180 can be modeled as a cantilever beam in bending. Classical mechanics of materials techniques can be used to evaluate material stresses as the beam undergoes bending, as occurs when couplers are engaged and disengaged. In addition, material stress can be analyzed for fatigue strength when bending is repeated or cyclic to provide sufficient product life. Increasing the width at the base of the beam as well as increasing the associated radius are geometric modifications used to reduce stress and improve strength.

Device aspect

In one aspect, the pipette tip coupling device or expandable mandrel collet coupling device 100 provides improved longevity.

In another aspect, the radially outwardly projecting segments 200 of the expandable mandrel collet 170 provide a stiffer coupling to provide a stiffer joint between the pipette tip 220 and the coupler 100. .

In another aspect, the radially outwardly projecting segments 200 of the expanding mandrel collet 170 pull the tip 220 up and effectively seat it.

In another aspect, the coupler 100 is unaffected by ejecting the tip into free air. O-ring coupling life is adversely affected when the tip is ejected into free air. This is because the O-ring is worn away by the grooves in the tip when the tip is pushed out by the spring-loaded eject sleeve. The hardness of the radially outwardly projecting segments 200 resists the detrimental effects of this galling and wear.

In another aspect, the material of the expandable mandrel collet 170 can be readily made from an electrically conductive material to include electrical circuitry on the chip for liquid level detection or other uses, as detailed above. It is designed to provide.

In another aspect, the radially outwardly projecting segments 200 of the expandable mandrel collet 170 are formed from a rigid, highly durable material such as, but not limited to, a metallic material or a rigid plastic material. , providing improved life, and because the individual elements or segments are much harder than plastic tips, they are more efficient than soft elastomeric materials (e.g., O-rings, etc.) in the tip grooves. work into

In another aspect, the radially outwardly projecting segments 200 can be activated by low compression/axial forces. The reason is that the mechanical design is efficient. Lower compression/axial force requirements improve the life of the associated components providing the axial force. As a result of this lower compression/axial force requirement, the radially outwardly projecting segments 200 enable the lower or distal seal 140 to have an improved life span. The reason is that elastomeric materials are not so compressed.

In another aspect, the coupler 100 allows the lower or distal seal 140 to be easily accessed should replacement be required. Also, the lower or distal seal 140 can be made from a wider variety of materials. The reason is that it does not have to be conductive for the LLD circuit.

In another aspect, maintenance costs are lower due to improved longevity and easier accessibility to the lower or distal seals.

In yet another aspect, tip alignment with respect to pipette device 20 is improved due to the improved seating.

Method aspect

In light of the above and in a further aspect, an illustration of a method for securing at least one pipette tip attachment to at least one pipette tip coupler in the form of an expanding mandrel collet coupling device carried by a pipette device. In an exemplary embodiment, the method comprises the steps of: (1) providing a pipette tip, the pipette tip including a sidewall having an interior surrounding surface defining a passage opening, the passage opening defining a pipette extending between an open distal end intended for immersion in the medium to be treated and an open proximal end axially opposite the open distal end. (2) providing a pipette tip coupler, the pipette tip coupler including a distally directed axial stop shoulder surface, the distally directed axial stop shoulder surface; is formed by an axially stepped coupler shoulder on the outer surrounding surface of the pipette tip coupler, and a distally directed axial stop shoulder surface is axially stepped on the inner surrounding surface of the pipette tip sidewall. (3) providing a plurality of separate coupling elements or segments, wherein a plurality of are circumferentially spaced apart and disposed on the upper seating surface of the pipette tip coupler body; (4) a distal elastomeric element; wherein the distal elastomeric element is carried by the pipette tip coupler at a location underlying the axially stepped coupler shoulder; (5) the center of the pipette tip coupler; positioning the distal end of the pipette tip coupler over the open proximal end of the pipette tip with axial alignment between the longitudinal axis and the central longitudinal axis of the pipette tip; (6) a circumferential radially inwardly angled inner surrounding surface of the pipette tip sidewall distal from the axially stepped shoulder of the inner surrounding surface of the pipette tip sidewall; A distally extending inner working surface is contacted by a distal elastomeric element (7) a sidewall of the pipette tip at a location overlying the axial stop surface of the pipette tip; radially abutting an axially arcuate circumferential surface sector portion above the axially arcuate circumferential inner surface defining a groove formed into the inner surrounding surface of the axially compressing or pushing a plurality of individual coupling elements or segments into an extended state, the resulting proximally directed radial and axial prestressing forces; to the pipette tip to excite the distal elastomeric element into a compressed state, the compressed state being applied to an outer circumferential portion of the distal elastomeric element and a sidewall of the pipette tip. configured to provide axial and radial sealing abutment with a circumferentially radially inwardly angled and distally extending inner working surface of the inner surrounding surface of the portion; abutting a proximally facing axial stop surface of the pipette tip against a distally facing axial stop surface of the pipette tip coupler body to define an axial coupling location of the pipette tip on the pipette tip coupler device; A method is provided comprising the steps of:

In light of this disclosure as described above, further structural modifications and adaptations may be made without departing from the scope and fair meaning of the embodiments of this disclosure as described above. . For example, FIGS. 57-67 show different alternative illustrations for the circumferential annular tip groove 246 shown at least in FIG. 19 and the V-shaped groove segment 200 shown at least in FIG. 1 is a fragmentary longitudinal cross-sectional side view detailing an exemplary embodiment; FIG. In particular, FIGS. 57-67 illustrate alternative groove configurations 2251-2261, respectively, for receiving segment 200. FIG. Additionally, the coupler segments can include radially outwardly facing surfaces that are complementary to different alternate exemplary embodiments of each of the respective groove configurations 2251-2261. Accordingly, the first work surface is in the form of, but not limited to, each of the grooved or uninterrupted configurations illustrated in FIGS. The substantially cylindrical interior surface section 242 of is devoid of interruptions, thereby defining an uninterrupted interior surface section 1242 of disposable pipette tip 1220 . Moreover, the tip distal O-ring sealing seat 270 can have different geometries in the form of, but not limited to, flat cones, concave radii, convex radii, steps, and the like. Moreover, the distal O-ring can have alternative shapes other than O-rings and can be in the form of a complementary configuration to the tip distal O-ring sealing seat 270, including but not limited to. It is possible.

Industrial applicability

The above depictions of systems, assemblies, devices, and methods, including uses and operations, demonstrate the industrial applicability of the disclosed embodiments.

Accordingly, many more structural modifications and adaptations are possible within the scope and fairness of the embodiments of the present disclosure, as described above and hereinafter described by the claims. It should be clear that this can be done without departing from the meaning. Therefore, the spirit and scope of the appended claims should not be limited to the description of the embodiments of the disclosure depicted above. And, in the appended claims, references to elements in the singular are not intended to mean "the only," but rather "one," unless expressly stated to do so. intended to mean "one or more". Moreover, a device or method need not address each and every problem sought to be solved by this disclosure in order for it to be covered by the present claims.

Alternative Pipette Device Assembly 3010

70-75 illustrate an alternative exemplary embodiment of a pipetting device assembly 3010, which includes an exemplary embodiment of pipetting device 3020, an exemplary embodiment of nozzle 3102, and an exemplary embodiment of nozzle 3102. 3102 and an exemplary embodiment of a leaf spring coupling device 3100 or pipette tip coupler for use with a disposable pipette tip 220 removably coupled to a pipette device 3020 via a leaf spring coupling device 3100 .

pipette device 3020

71 and 72, a pipetting device 3020 includes a body portion 3022 that supports an aspiration and dispensing device 3024 that includes a plunger 3026. , plunger 3026 is operatively coupled to and driven by motor 3028 . The plunger 3026 resides within a plunger cylinder 3030 that extends from the distal or lower end 3032 of the body portion 3022 of the pipette device 3020 .

Pipette device 3020 further includes an aspiration and dispensing cylinder 3034 that is positioned axially aligned with and distally below plunger 3026 to plunger cylinder 3034 . 3030 at least partially. Plunger cylinder 3030 transitions distally to distal mounting flange 3036 for mounting nozzle 3102 . Leaf spring coupling device 3100 is connected at one end to nozzle 3102 and leaf spring coupling device 3100 is removably connected to disposable pipette tip 220 at the other end.

70, 71, 72, 77 and 79, nozzle 3102 includes aspiration and dispensing cylinder 3034. Referring to FIGS. Aspiration and dispensing cylinder 3034 further includes an inner enclosing sidewall 3038 defining an open-ended pipette channel 3040 extending therethrough. An open-ended pipette channel 3040 opens with an open upper end portion 3042 of the aspiration and dispensing cylinder 3034 to provide open communication between the plunger 3026 and the external area adjacent the distal mounting flange 3036. It extends longitudinally along the longitudinal channel axis 3080 of the pipette device assembly 3010 between the lower end portion 3044 and the lower end portion 3044 . Distal mounting flange 3036 is operably connected to nozzle 3102 , which in turn is connected to leaf spring coupling device 3100 . An open-ended central channel 3136 extends through nozzle 3102 and leaf spring coupling device 3100 to provide open communication between tip 220 and aspiration and dispensing cylinder 3034 .

plunger carriage 3063 and ejection sleeve 3062

70-74 , aspiration and dispensing device 3024 includes lead screw 3067 driven by motor 3028 . Lead nut 3054 is operably connected to lead screw 3067 . In one embodiment, lead nut 3054 is threaded and screwed onto lead screw 3067 . Plunger carriage 3063 surrounds and is operably connected to leadnut 3054 such that movement of motor 3028 drives leadnut 3054 and leadnut 3054 , parallel to the longitudinal channel axis 3080 to drive the plunger carriage 3063 .

Eject block 3065 surrounds lead screw 3067 and is positioned below plunger carriage 3063 . Eject rod 3069 is operably connected to the end of eject block 3065 . Eject rod 3069 extends from eject block 3065 through passageway 3021 through the distal or lower end 3032 of body portion 3022 of pipette device 3020 . An eject spring 3074 surrounds the eject rod 3069 . The eject spring 3074 is positioned between the distal end of the eject rod 3065 and the distal or lower end 3032 of the body portion 3022 of the pipette device 3020 such that the spring force is removed from the plunger carriage 3063. It acts in a direction to push eject block 3065 away.

The pipette device further includes an ejection sleeve 3062 that surrounds the plunger cylinder 3030 and nozzle 3102 and contains the aspiration and dispensing cylinders 3034 . Ejection sleeve 3062 is used to eject disposable pipette tip 220 from pipette device 3020 and is axially moveable relative to aspiration and dispensing cylinder 3034 and plunger cylinder 3030. , a proximal or upper end 3064, a distal or lower end 3066, and an ejection sleeve arm 3068, the ejection sleeve arm 3068 extending at a first end to an upper end. 3064 is attached to ejection sleeve 3062 and has an opposite second end that is removably attached to distal end 3071 of ejection rod 3069 .

Eject sleeve 3062 is free when pipette tip 220 is not attached (eg, after pipette tip 220 has been ejected). In order to install the pipette tip 220, the ejection sleeve spring force must be overcome to axially push the ejection sleeve 3062 into the retracted position as shown in FIGS. 71-73. . Moreover, the spring 3074 is sized to be long enough to assist in ejecting the pipette tip 220 until the pipette tip 220 is completely disengaged from the leaf spring coupling device 3100. is designed to provide the power of

Nozzle 3102 and leaf spring coupling device 3100

74-75 illustrate nozzle 3102 and leaf spring coupling device 3100, which are used to attach pipette tip 220 to pipette device 3020. FIG.

nozzle 3102

More specifically, the nozzle 3102 has a nozzle mounting portion 3103 positioned at the top end of the nozzle 3102, a nozzle stem portion 3107 positioned at the bottom end of the nozzle 3102 relative to the nozzle mounting portion, It includes a nozzle body portion 3105 positioned between a nozzle mounting portion 3103 and a nozzle stem portion 3107 and a nozzle elastomeric element 3135 . A nozzle mounting portion 3103 connects the nozzle 3102 to a distal mounting flange 3036 (FIGS. 71 and 79) of the pipette device 3020. FIG.

As further illustrated in FIGS. 75 and 79, nozzle 3102 further includes nozzle elastomeric element 3135 coaxially carried about nozzle stem portion 3107 of nozzle 3102 . Nozzle stem portion 3107 is positioned at the opposite end with respect to longitudinal centerline axis 3090 of nozzle 3102 from nozzle mounting portion 3103 . In an exemplary embodiment, nozzle stem portion 3107 further includes a nozzle groove 3109 and a nozzle elastomeric element is carried within nozzle groove 3109 . In an exemplary embodiment, nozzle elastomeric element 3135 is an O-ring.

Leaf spring coupling device 3100

As illustrated in FIGS. 74-77, leaf spring coupling device 3100 is carried on coupling cylinder 3173, leaf spring cylinder 3175, distal stem base 3121, and distal stem base 3121. and a distal elastomeric element or lower elastomeric element 3140 .

As shown in FIGS. 76 and 77, the lower end 3171 of coupling cylinder 3173 is connected to leaf spring cylinder 3175 . A leaf spring assembly 3170 is formed within a leaf spring cylinder 3175 . Upper annular stop shoulder end 3177 of leaf spring cylinder 3175 is located at the lower end of leaf spring assembly 3170 . Lower portion 3122 of leaf spring cylinder 3175 is connected to distal stem base 3121 .

As also shown in FIGS. 76 and 77, distal stem base 3121 includes a distal cylindrical stem portion surface 3124 , which includes upper annular stop shoulder end 3122 . to a rounded end plate 3126 having an upper surface 3128 and a lower surface defined by a distal or lower annular end surface 3130. ing. As shown, the end plate 3126 has a diameter greater than the diameter of the narrowest portion of the distal cylindrical stem portion surface 3124, the distal stem portion surface 3124 being the leaf spring coupling device. A distal groove portion 3132 of 3100 is defined. When the pipette tip 220 is coupled with the leaf spring coupling device 3100, the upper annular stop shoulder end 3177 will abut against the proximally-facing axial stop surface 260 of the pipette tip 220 and the leaf spring cup. Prevents ring device 3100 from being inserted too far into pipette tip 220 .

Distal elastomeric element 3140

As further illustrated in FIGS. 76 and 77, leaf spring coupling device 3100 further includes a distal or lower elastomeric element 3140, which is coaxially carried in distal stem portion 3124. . Distal elastomeric element 3140 acts as a seal when pipette tip 220 is coupled to leaf spring coupling device 3100 .

In one embodiment, and with reference to FIGS. 77 and 78, distal elastomeric element 3140 includes an annular body portion 3142 . Annular body 3142 includes an interior surface 3144 defining a central opening 3146 , a top surface 3148 , a peripheral exterior surface 3150 and a bottom surface 3152 . The central opening 3146 is sized to closely or closely surround the distal stem portion 3124 of the leaf spring coupling device 3100, while the groove 3132, as illustrated in FIG. and extends circumferentially radially outward beyond the end plate 3126 . In a relaxed or uncompressed state, distal elastomeric element 3140 includes a circumferentially continuous, generally circular cross-sectional area 3154, as illustrated in FIG.

leaf spring assembly 3170

76, 77 and 82, the leaf spring assembly 3170 includes a plurality of circumferentially spaced leaf springs 3180 formed within a leaf spring cylinder 3175. , arranged parallel to the central longitudinal axis 3090 and separated by open vertical slots 3182 . Leaf spring 3180 is flexible. Each pair of adjacent leaf springs 3180 is separated by an open vertical slot 3182 . Leaf spring 3180 is flexible and is used to hold the pipette tip onto leaf spring coupling device 3100 .

Each leaf spring 3180 includes a retention bump 3202 that protrudes from an outer surface 3185 of leaf spring 3180 . In one embodiment, each of the plurality of retention bumps 3202 has a rounded surface protruding from the outer surface 3185 . A plurality of retention bumps 3202 on the leaf spring assembly 3170 extend into the grooves 246 of the pipette tip 220 when the pipette tip is coupled to the leaf spring coupling device 3100 , and the leaf spring coupling device 3100 . Keep (or hold) the pipette tip 220 on top.

Each leaf spring 3180 further includes a stabilizer plateau 3183 that protrudes from an outer surface 3185 of leaf spring 3180 . A plurality of stabilizer plateaus 3183 on the leaf spring assembly 3170 prevent the pipette tip from rocking when the pipette tip is coupled to the leaf spring coupling device 3100 . A plurality of stabilizer plateaus 3183 prevent tip rotation on leaf spring coupler 3100 around retention bumps 3202 positioned in grooves 246 of pipette tips 220 . Such rocking or rotation contributes to misalignment between the end of pipette tip 220 and central longitudinal axis 3090 . Additionally, this type of rocking can cause the pipette tip seal to dislodge when a lateral load is applied. To prevent this problem, in one embodiment, the plurality of stabilizer plateaus 3183 are positioned above the retention bumps 3202 or closer to the coupling cylinders 3173 relative to the retention bumps 3202 . is installed on top of Plurality of stabilizer plateaus 3183 have an interference fit with pipette tip 220 when retention bumps 3202 expand into grooves 246 of pipette tip 220 . A plurality of stabilizer plateaus 3183 add another point of contact between the pipette tip 220 and the leaf spring coupling device 3100 to prevent tip rocking.

Pipette tip pick-up process with leaf spring coupling device 3100

FIGS. 81-85 illustrate details of an exemplary embodiment of successive stages of a pipette tip pick-up process, including, among other things, a leaf spring coupling device 3100 operably carried by a pipette device 3020. , illustrate how the pipette tip 220 attachment is secured. As noted above, in exemplary embodiments, pipette tip 220 may be supported by support surface 282 .

As illustrated in FIGS. 73, 77 and 81, leaf spring coupling device 3100 is connected to device 3020 via nozzle 3102 and upon command, leaf spring coupling device 3100 will , are positioned above the open proximal end 232 of the pipette tip 220 and each of their respective central longitudinal axes are aligned along the Z-axis. Leaf spring 3180 is in a relaxed state. Eject sleeve spring 3074 pushes eject block 3065 and eject sleeve 3062 to their lowest position. Plunger carriage 3063 is positioned up to allow eject block 3065 to move up during the pipette tip pick-up process. Distal elastomeric element 3140 is in an uncompressed state.

73, 76, 82, 83, and 84 show leaf spring coupling device 3100 being moved down into pipette tip 220 along the Z-axis (FIG. 81). , lowering the distal elastomeric carrying portion of the leaf spring coupling device 3100 into the inner cylindrically shaped proximal end portion of the pipette tip 220 and uncompressing the distal elastomeric element 3140. while maintaining distal elastomeric element 3140 in contact with annular sealing seat 270 of pipette tip 200 . Leaf spring 3180 has entered pipette tip 220 and is in compression. The pipette tip pushes eject sleeve 3062 and eject block 3065 up. At this point, a gap 3298 exists between the axial stop surface 260 of the pipette tip 220 and the upper annular stop shoulder end 3177 of the leaf spring coupling device 3100, as detailed in FIG. ing. Additionally and with reference to FIGS. 81 and 82, retention bumps 3202 are shown beginning to move into grooves 246 of pipette tip 220 .

77 and 85, leaf spring coupling device 3100 is then pushed into pipette tip 220 along the Z-axis until leaf spring coupling device 3100 is firmly seated against sealing seat 270 of pipette tip 220 . It shows that it can be moved further down. A retention bump 3202 on leaf spring 3180 reaches and snaps into groove 246 on pipette tip 220 , locking the pipette tip in place on leaf spring coupling device 3100 . Distal elastomeric element 3140 compresses against sealing seat 270 in pipette tip 220 . The stabilizer plateau is engaged with the tip and prevents the pipette tip from rotating on the leaf spring coupling device 3100 .

Upon completion of the attachment securing process detailed above, the plurality of leaf springs 3180 and distal elastomeric elements 3140 work in combination to create a segment and seal coupling that provide a fluid-tight seal, resulting in a plurality of The retention bumps 3202 are at least partially received within the grooves 246 of the pipette tip 220 and on the circumferentially arcuate interior surface 244 (FIG. 18) that defines the grooves 246 of the pipette tip. At least partially seated, distal elastomeric element 3140 seals against sealing seat 270, which is angled radially inward and extends distally or downwardly. providing the surface.

Disposable Pipette Tip Ejection Process with Leaf Spring Coupling Device 3100

81-85 conversely illustrate details of sequential steps of an exemplary method or process for ejecting a pipette tip 220 from a leaf spring coupling device 3100 operably carried by a pipette device 3020. ing. This tip ejection process sequence is similar to the attachment or tip pickup fixation process sequence, except in reverse.

As illustrated in FIGS. 72, 73, and 81-85, in one exemplary embodiment, the ejection process includes rotating lead screw 3067, thus causing plunger carriage 3063 to rotate. is driven downward into eject block 3065 . Eject block 3065 pushes eject sleeve 3062 downward through its attachment to eject rod 3069 . Eject sleeve 3062 begins pushing pipette tip 220 out of leaf spring coupling device 3100 . The pipette tip 220 will not move until the force is sufficiently great that the leaf spring 3180 (FIG. 77) compresses and allows the retention bumps 3202 to move out of the grooves 246 in the pipette tip 220. not start.

Leaf spring 3180 is then fully compressed into pipette tip 220 . Pipette tip 220 is no longer held vertically in leaf spring coupling device 3100 . At this point in the ejection process, the distal elastomeric element 3140 has lost contact with the sealing seat 270, thus breaking the seal. Plunger carriage 3063 continues to drive ejection sleeve 3062 down, pushing pipette tip 220 out of leaf spring coupling device 3100 .

Pipette tip 220 is then continued to be driven out of leaf spring coupling device 3100 . Retention bump 3202 reaches the opening of pipette tip 220 and leaf spring 3180 begins to expand toward its relaxed state.

Upon completion of the ejection process, pipette tip 220 has lost contact with leaf spring coupling device 3100 . Leaf spring 3180 is in a relaxed state. Eject sleeve spring 3074 is pushing against eject block 3065 and eject sleeve 3062 is in its lowest position. After the pipette tip 220 is ejected, the plunger carriage 3063 is positioned up to allow the eject block 3065 space to move up during the next pipette tip pickup process.

Coupling and Ejection Forces for Leaf Spring Coupling Device 3100

86 illustrates a schematic vector diagram of a plurality of retention bumps 3202 of leaf spring coupling device 3100 extending initially into groove 246, with retention bumps 3202 on leaf spring 3180 extending into groove 246. FIG. , above the center of the retention bump radius, comes into contact with the upper corner of the tip groove 246, resulting in an axial upward force that pulls the pipette tip 220 upward. As illustrated in FIG. 86, the retention bump force (or segment force (Fsegment_resultant)) for each of the plurality of retention bumps 3202 is composed of two components: an axial force (Fsegment_axial) component and a radial force (Fsegment_resultant) component. directional force (Fsegment_radial component).

As long as the plurality of retention bumps 3202 contact the upper corners of the chip groove 246 above the center of the retention bump radius (dimension Z in FIG. 87), the corners of the center of the retention bump radius and the groove 246 are aligned. Fsegment_axial increases as the distance between the segments increases. Thus, at the beginning of the pipette tip pick-up process, the segment axial force (Fsegment_axial) component starts low, as illustrated in FIG. 86 and detailed in FIG. increases to its maximum at the end of the pipette tip pick-up process, as shown in FIG.

Referring to FIG. 87, the ratio of Z/R is equal to SIN(ω), and SIN(ω) is equal to (Fsegment_axial)/(Fsegment_resultant). As a result, (Fsegment_axial) is equal to (Fsegment_resultant) multiplied by the ratio of Z/R. From this, the result is that (Fsegment_axial) increases as Z increases.

Referring to FIG. 88, the segment axial force (Fsegment_axial) component seats the upper annular stop shoulder end 3177 against the axial stop surface 260 of the pipette tip 220, causing the O-ring (or distal elastomer ) provides the force required to overcome the axial force (Fdistal_ring_axial) component, compressing the distal elastomeric element (or O-ring) 3140 . O-ring 3140 has an O-ring force (Fdistal_ring_resultant) resulting from being compressed, which has two components, an axial component (Fdistal_ring_axial) and a radial component (Fdistal_ring_radial). )including. Additionally, the segment radial force (Fsegment_radial) component provides the radial force required to lock the retention bump 3202 into the tip groove 246 (FIG. 18) and the distal O-ring radial force Component (Fdistal_ring_radial) provides the radial force required to maintain the seal against pipette tip 220 . In addition, the retention bump-to-chip groove geometry causes Fsegment_axial to increase (increase dimension Z) as retention bump 3202 enters groove 246, so that the O-ring axial force component (Fdistal_ring_axial) To help overcome, the distal O-ring 3140 can be fully compressed to the desired degree. Additionally, the distal O-ring axial force component (Fdistal_ring_axial) provides force to help remove tip 220 during the ejection process.

Alignment/Misalignment for Leaf Spring Coupling Device 3100

Upper annular stop shoulder end 3177 of coupler 3100 and axial stop surface 260 of tip 220 are critical for correct tip alignment. Thus, leaf spring coupling device 3100 and pipette tip 220 are configured such that multiple retention bumps 3202 push upper annular stop shoulder end 3177 and axial stop surface 260 together to prevent misalignment. . The reason is that if the upper annular stop shoulder end 3177 and the axial stop surface 260 are not properly mated, especially if they are tilted, a misalignment error (E) (which is , which is the same as illustrated for the embodiment shown in FIG. 36) can become significant.

Dimensions and Relationships for Leaf Spring Coupling Device 3100

Therefore, the dimensions between leaf spring coupling device 3100 and tip 220 are related for proper use and operation.

38, 79 and 85, tip groove diameter A is sufficient to allow retention bumps 3202 to pull tip 220 up and properly lock tip 220 in place. must have grown to Conversely, if the tip groove diameter A is too large, the retention bumps 3202 may not be pushed in enough to obtain a good lock. Additionally, inner diameters B and C must be greater than outer diameters K and L, respectively, of upper annular stop shoulder end 3177 . However, they should not be too large. The reason is that this can result in a poor fit and/or misalignment.

38 and 85, the tip seat to groove dimension S must be matched to the dimension M from the upper annular stop shoulder end 3177 to the segment 200 center. This relationship is important to the connection between tip 220 and upper annular stop shoulder end 3177 .

19, 38 and 85, the dimension from the axial stop surface 260 in FIG. 19 to the O-ring seal land 266 (dimension F in FIG. 38) is the upper annular stop shoulder end Must match vertical lip surface 3179 (dimension N in FIG. 85) distally from 3177 . These dimensions control the amount that the distal O-ring 3140 is compressed and therefore how well it seals. Axial stop surface 260 and upper annular stop shoulder end 3177 must be fully mated to provide proper alignment and to maintain chip axial distance D. FIG.

38 and 85, the dimension D (or axial distance) from the axial stop surface 260 to the distal end 230 establishes a known and controlled distance of the pipette tip end. This is important to allow the pipette device to target small bores and small volumes of liquid. Additionally, smaller volumes of liquid can be transferred, resulting from a known fixed distance of the pipette tip, and the pipette to the work surface onto which or from which the liquid will be transferred. Allows controlled tip/liquid contact.

38, 79, and 85, tip internal diameter G is greater than diameter L of the leaf spring coupling device to create a seat or land for distal O-ring 3140 to seal against. should also be smaller. If the diameter G is too large, the distal O-ring may not seal well. If the diameter is too small, the distal O-ring 3140 may not fully compress and may prevent the upper annular stop shoulder end 3177 from seating, or the distal O-ring may May cause harm to 3140. Additionally, along with diameter G, ramp length H controls the seat or land that mates with O-ring 3140 . These dimensions are important in providing a good O-ring seal. If the ramp length H is too long, the O-ring may not seal well. If H is too short, the O-ring may not fully compress and may prevent the upper annular stop shoulder end 3177 from seating or may cause damage to the O-ring 3140. may occur.

liquid level detection

Pipette device assembly 3010 further includes a liquid level detection circuit assembly as described with respect to pipette device assembly 10 (FIG. 40). In an exemplary embodiment, the materials of nozzle 3102 and leaf spring coupling device 3100 can be readily made from electrically conductive materials to facilitate liquid level detection or other applications, as detailed above with respect to pipette device assembly 10. provides electrical circuitry to the pipette tip 220 for use in .

Alternate Exemplary Embodiment

FIG. 90 illustrates an exemplary embodiment of a leaf spring coupling device 3100 positioned within a disposable pipette tip that includes an alternative sealing seat surface 2270, the tip 220 being its final seating surface. 2270, with the distal elastomeric element 3140 in its final compressed and seated sealing condition against the alternative sealing seat surface 2270.

91 details the final compression of distal elastomeric element 3140 against alternative sealing seat surface 2270. FIG.

Claims (5)

A pipette tip coupling device for coupling a pipette tip to a pipette device, said pipette tip coupling device comprising:
including a nozzle,
The nozzle is
a nozzle mounting portion positioned at the upper end of the nozzle;
a nozzle stem portion positioned at the bottom end of the nozzle;
a nozzle body portion positioned between the nozzle mounting portion and the nozzle stem portion;
a nozzle elastomeric element surrounding said nozzle stem portion;
Further, the pipette tip coupling device is
a leaf spring coupling device connected to the bottom end of the nozzle;
The leaf spring coupling device comprises:
a coupling cylinder for receiving said nozzle;
including a leaf spring cylinder and
The leaf spring cylinder is
a leaf spring assembly;
including a lower portion and
The leaf spring assembly includes:
A plurality of leaf springs, each leaf spring comprising:
external surface;
stabilizer plateaus projecting from said outer surface; and
a plurality of leaf springs including retention bumps with rounded surfaces protruding from said outer surface;
an upper annular stop shoulder end positioned at the lower end of the leaf spring cylinder;
Further, the leaf spring coupling device is
a distal stem base connected to the lower portion of the leaf spring cylinder;
the distal stem base comprising:
a distal cylindrical stem portion surface;
an end plate;
a distal groove portion and
the distal cylindrical stem portion surface transitions from the upper annular stop shoulder end to the rounded end plate forming the distal groove portion;
Further, the pipette tip coupling device is
a distal elastomeric element disposed about the distal stem base;
an open-ended central channel forming an open passageway extending longitudinally from the top of the nozzle through the distal stem base of the leaf spring coupling device;
The nozzle stem portion is inserted into the leaf spring coupling device and abuts an interior surface of the leaf spring coupling device to form a seal between the nozzle and the leaf spring coupling device. pipette tip coupling device. 2. The pipette tip coupling device of claim 1, wherein said nozzle stem portion of said nozzle further comprises a nozzle groove, said nozzle elastomeric element being carried within said nozzle groove. 2. The pipette tip coupling device of claim 1, wherein said nozzle elastomeric element comprises an O-ring and said distal elastomeric element comprises an O-ring. 2. The pipette tip coupling device of claim 1, wherein the nozzle and the leaf spring coupling device each further comprise electrically conductive material. 4. The plurality of leaf springs are configured to radially contract to a relaxed state when pressure is applied and radially expand when pressure is released. 2. The pipette tip coupling device according to 1.

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