JP2023155210A - 高密度空間マルチオミクスのための、ｉｎ ｓｉｔｕ標的捕捉および空間的固有分子識別子（ＳＵＭＩ）識別と、ｉｎ ｖｉｔｒｏ配列決定とを組み合わせる方法 - Google Patents

高密度空間マルチオミクスのための、ｉｎ ｓｉｔｕ標的捕捉および空間的固有分子識別子（ＳＵＭＩ）識別と、ｉｎ ｖｉｔｒｏ配列決定とを組み合わせる方法 Download PDF

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JP2023155210A
JP2023155210A JP2023062354A JP2023062354A JP2023155210A JP 2023155210 A JP2023155210 A JP 2023155210A JP 2023062354 A JP2023062354 A JP 2023062354A JP 2023062354 A JP2023062354 A JP 2023062354A JP 2023155210 A JP2023155210 A JP 2023155210A
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oligonucleotide
sequence
sumi
sequencing
target
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JP2023155210A5 (https=
Inventor
ロートマン トーマス
Rothmann Thomas
ピナード ロバート
Pinard Robert
正裕 細野
Masahiro Hosono
ボズィオ アンドレアス
Bosio Andreas
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Miltenyi Biotec GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
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  • Microbiology (AREA)
  • Molecular Biology (AREA)
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  • Biotechnology (AREA)
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  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2023062354A 2022-04-07 2023-04-06 高密度空間マルチオミクスのための、ｉｎ ｓｉｔｕ標的捕捉および空間的固有分子識別子（ＳＵＭＩ）識別と、ｉｎ ｖｉｔｒｏ配列決定とを組み合わせる方法 Pending JP2023155210A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP22167058.1A EP4257702A1 (en) 2022-04-07 2022-04-07 Method combining in situ target capture and spatial unique molecular identifier (sumi) identification with in vitro sequencing for high density spatial multiomics
EP22167058 2022-04-07

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JP2023155210A true JP2023155210A (ja) 2023-10-20
JP2023155210A5 JP2023155210A5 (https=) 2026-04-13

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JP2023062354A Pending JP2023155210A (ja) 2022-04-07 2023-04-06 高密度空間マルチオミクスのための、ｉｎ ｓｉｔｕ標的捕捉および空間的固有分子識別子（ＳＵＭＩ）識別と、ｉｎ ｖｉｔｒｏ配列決定とを組み合わせる方法

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US (1) US20230323438A1 (https=)
EP (1) EP4257702A1 (https=)
JP (1) JP2023155210A (https=)
CN (1) CN116891889A (https=)

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EP0929683A1 (en) * 1996-03-06 1999-07-21 ZymoGenetics, Inc. DIABETES MELLITUS 37 kD AUTOANTIGEN PROTEIN-TYROSINE PHOSPHATASE
ES2216080T3 (es) 1996-05-29 2004-10-16 Walter Dr. Schubert Dispositivo automatizado y procedimiento para medir y determinar moleculas o partes de moleculas.
US6031098A (en) * 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
ATE245278T1 (de) 1999-11-04 2003-08-15 Meltec Multi Epitope Ligand Te Verfahren zur automatischen analyse von mikroskopaufnahmen
DE10014685B4 (de) 2000-03-24 2004-07-01 Schubert, Walter, Dr. Verfahren zur Identifizierung von zellspezifischen Zielstrukturen
DE10043470B4 (de) 2000-09-04 2006-01-19 Mpb Meltec Patent- Und Beteiligungsgesellschaft Mbh Verfahren zur Identifizierung von zellspezifischen Proteinen
US7253341B2 (en) * 2001-03-05 2007-08-07 Yissum Research Development Company Of The Hebrew University Of Jerusalem Denaturant stable and/or protease resistant, chaperone-like oligomeric proteins, polynucleotides encoding same, their uses and methods of increasing a specific activity thereof
SG170028A1 (en) * 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
US8633139B2 (en) * 2007-12-21 2014-01-21 Abbvie Biotherapeutics Inc. Methods of screening complex protein libraries to identify altered properties
US10526649B2 (en) * 2015-04-14 2020-01-07 Massachusetts Institute Of Technology Augmenting in situ nucleic acid sequencing of expanded biological samples with in vitro sequence information
JP6967006B2 (ja) * 2016-01-15 2021-11-17 クアンタポール， インコーポレイテッド 低減されたバックグラウンドの光学に基づくナノポア分析
US20210207131A1 (en) 2016-02-18 2021-07-08 President And Fellows Of Harvard College Multiplex Alteration of Cells Using a Pooled Nucleic Acid Library and Analysis Thereof
GB2570412A (en) 2016-08-31 2019-07-24 Harvard College Methods of generating libraries of nucleic acid sequences for detection via fluorescent in situ sequencing
WO2020076976A1 (en) * 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing

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CN116891889A (zh) 2023-10-17
EP4257702A1 (en) 2023-10-11
US20230323438A1 (en) 2023-10-12

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