JP2023097853A - Bait composition for insects of vespidae, pest control container for insects of vespidae comprising the bait composition, and pest control method for insects of vespidae - Google Patents

Abstract

To provide a bait composition for insects of Vespidae for pest control of a nest of insects of Vespidae, by the bait composition, after hornets eat the bait composition on a bait place, the hornets fly to the nest, a colony of the nest of the hornets can be collapsed by nutrient exchange, and to provide a pest control method using the same composition.SOLUTION: There is provided a bait composition in which a poison bait including an insecticidal component for insects of Vespidae is blended. The insecticidal component includes either one or more of a boric acid compound, dinotefuran, fipronil, and if the insecticidal component is the boric acid compound, the boric acid compound is included by 0.5 mass% or more and 15 mass% or less to a total amount of the bait composition, if the insecticidal component is the dinotefuran, the dinotefuran is included by 0.3 ppm to 100 ppm to the total amount of the bait composition, and if the insecticidal component is the fipronil, the fipronil is included by 0.1 ppm to 100 ppm to the total amount of the bait composition.SELECTED DRAWING: None

Description

The present invention relates to a bait composition for Waspidae insects. In particular, the present invention relates to a bait composition for controlling nests of Waspidae insects, a control container provided with the bait composition, and a control method.

Insects belonging to the family Waspidae (particularly the subfamily Waspidae), which are relatively large among bees, have the property of attacking violently. It is known that when stung, toxic substances circulate throughout the body and can cause acute allergic reactions.
Wasp insects build nests and lay eggs in them. Nests are important bases for wasps, where larvae hatch, grow, pupate, emerge, and become adults. Insects get agitated and attack ferociously.
Therefore, various measures have been taken for the control of Waspidae insects. For example, a method of killing insects with an aerosol (Patent Document 1) and traps (Patent Documents 2 and 3) are known.

However, the method of killing insects with an aerosol will irritate the nest, and there is a danger that the wasps will get excited and attack violently. There was a problem of taking In addition, the method of catching wasps flying outside the nest cannot control the growing eggs, larvae, and imagoes immediately after emergence in the nest, which is problematic in that it is not fundamentally exterminating them. . Furthermore, 50 to 100 wasps form a colony in one nest and form a swarm, and there is a problem that even if the wasps that accidentally fly to the trap are exterminated, they cannot be effectively exterminated. Furthermore, when the location of the nest was not known, extermination was even more difficult.

On the other hand, methods using poisonous baits have also been proposed for controlling insects such as ants and cockroaches. In other words, the insecticidal component is added to the bait, and the poisonous bait is carried to the nest to exterminate the entire nest.
However, unlike the extermination of ants and cockroaches, in the control of vespidae insects, which are flying insects, the control container equipped with the bait composition containing the insecticidal component is allowed to fly to the feeding place (feeding place) to feed. Furthermore, a bait composition that can fly from a feeding place to a nest, exchange nutrients (food exchange) in the nest, and then collapse the colony of the nest, and a control method using the bait composition is required.

JP 2008-156235 A Registered Utility Model No. 3201021 JP 2014-103933 A

The present invention provides a bait composition for Waspidae insects, which can fly to the nest after eating the bait composition at a feeding site and can collapse the colony of the nest by nutrient exchange in order to control the nest of Waspidae insects. An object of the present invention is to provide a control method using the bait composition.

How to solve the problem

The present inventors have made intensive studies in view of the above circumstances, and as a result, by appropriately blending the insecticidal component of the Waspidae insect, the Waspidae insect can fly to the nest after eating the bait composition at the feeding place, In addition, the present inventors have found a bait composition capable of collapsing a nest colony through nutrient exchange by returning Waspidae insects.

That is, the bait composition of the present invention is a bait composition containing an insecticidal component for a Waspidae insect, wherein the insecticidal component contains any one of a boric acid compound, dinotefuran, and fipronil as an active ingredient, and When the insecticidal component is a boric acid-based compound, the boric acid-based compound is 0.5% by mass to 15% by mass relative to the total amount of the bait composition, and when the insecticidal component is dinotefuran, the dinotefuran is 0 relative to the total amount of the bait composition. .3 ppm to 100 ppm, and when the insecticidal component is fipronil, the amount of fipronil is 0.1 ppm to 100 ppm with respect to the total amount of the bait composition.

The bait composition may further comprise an attractant for Waspidae insects.

The vessel for controlling Waspidae insects of the present invention comprises the above bait composition, and has a structure in which the Waspidae insects can eat the bait composition and can leave after eating.

In the method for controlling Waspidae insects of the present invention, an insecticidal component is blended to prepare a bait composition, and the bait composition is placed in a control container having a structure in which the Waspidae insects can eat and can leave after eating. is set, and the control container is placed at a predetermined distance from the nest of the Waspidae insect, wherein the insecticidal component is a boric acid compound, dinotefuran, and fipronil as active ingredients. When the insecticidal component is a boric acid compound, the boric acid compound is 0.5% by mass to 15% by mass with respect to the total amount of the bait composition, and when the insecticidal component is dinotefuran, the bait composition Dinotefuran is 0.3 ppm to 100 ppm relative to the total amount, and when the insecticidal component is fipronil, fipronil is 0.1 ppm to 100 ppm relative to the total amount of the bait composition.

The bait composition in the control method may further contain a component that attracts Waspidae insects.

By using the bait composition, the control container equipped with the bait composition, and the control method using the bait composition of the present invention, Waspidae insects can fly to the nest after eating the bait composition at the feeding place, and The nest colony can be destroyed by nutrient exchange by homing wasps. That is, simply by placing the bait composition at a feeding site, it is possible to destroy the nest colony and control the entire nest without directly stimulating the Waspidae insects.

The control container used for the evaluation test is shown. FIG. 2 is a schematic view of the location of each nest and the arrangement of control containers in which the bait composition is set in Evaluation Test 1. FIG. 1 is a photograph of a hornet's nest when a bait composition containing 5.0 ppm of dinotefuran was set in Evaluation Test 1. FIG. 1 is a dismantled photograph of a hornet's nest when a bait composition containing 5.0 ppm of dinotefuran was set in Evaluation Test 1. FIG. FIG. 2 is a schematic diagram of the arrangement of the locations of nests and the control containers set with the bait composition in Evaluation Test 2. FIG. 10 is a photograph of a hornet's nest when a bait composition containing 5.0 ppm of dinotefuran was set in Evaluation Test 2. FIG. Fig. 10 is a photograph of the condition of a hornet's nest when a bait composition containing 5.0 ppm of dinotefuran was set in Evaluation Test 2. Fig. 10 is a photograph of the condition of a hornet's nest when a bait composition containing 5.0 ppm of dinotefuran was set in Evaluation Test 2. 10 is a dismantled photograph of a hornet's nest when a bait composition containing 10 ppm of fipronil was set in Evaluation Test 3. FIG.

Embodiments of the present invention will be specifically described below.

(bait composition)
The bait composition of the present invention is a poison bait containing an insecticidal component of Waspidae insects, and contains at least the insecticidal component described later.

Waspidae insects include wasps, paper wasps, and wasps. Wasps belong to the subfamily Waspidae among insects belonging to the family Waspidae, and exemplified by the hornet wasp and the yellow wasp.
After eating the bait composition, the Waspidae insects return to the nest and carry food to the larvae and the like in the nest, and the adults exchange nutrients with each other. In such nutrient exchange, the insecticidal component in the bait composition is also exchanged together, larvae living in the same nest, nest-based adults die, and larvae that hatch from eggs thereafter are not fed. to die.

(insecticidal component)
The insecticidal component contained in the poison bait blended in the bait composition is a component that kills vespidae insects. It is capable of collapsing the imagoes that enter and exit together with the colony.
Insecticidal components for Waspidae insects include boric acid (orthoboric acid, metaboric acid, boronic acid, boric acid, perboric acid, hypoboric acid) and salts thereof, disodium octaborate tetrahydrate (Timboa), and the like. Boric acid compounds, dinotefuran ((RS)-1-methyl-2-nitro-3-(tetrahydro-3-furylmethyl)guanidine), fipronil (5-amino-1-[2,6-dichloro-4-( trifluoromethyl)phenyl]-4-trifluoromethylsulfinyl)pyrazole-3-carbonitrile), silafluofene (4-ethoxyphenyl[3-(4-fluoro-3-phenoxyphenyl)propyl]dimethylsilane), pyriprole (1- [2,6-dichloro-4-(trifluoromethyl)phenyl]-4-[(difluoromethyl)thio]-5-[(2-pyridinylmethyl)amino]-1H-pyrazole-3-carbonitrile), hydramethyl Non (N-[1,5-bis[4-(trifluoromethyl)phenyl]pent-1,4-dien-3-ylideneamine]-5,5-dimethyl-4,6-dihydro-1H-pyrimidine-2 -amine), permethrin ((3-phenoxybenzyl=(1RS,3RS)-(1RS,3RS)-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate), cypermethrin (( RS)-α-cyano-3-phenoxybenzyl = (1RS,3RS)-(1RS,3RS)-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate), fenvalerate (α -cyano-3-phenoxybenzyl-2-(4-chlorophenyl)-3-methylbutanoate), bifenthrin (2-methylbiphenyl-3-ylmethyl (Z)-(1RS,3RS)-3-(2-chloro -3,3,3-trifluoroprop-1-enyl)-2,2-dimethylcyclopropanecarboxylate), etofenprox (2-(4-ethoxyphenyl)-2-methylpropyl 3-phenoxybenzyl ether) ), cyfluthrin (cyano (4-fluoro-3-phenoxyphenylmethyl-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarboxylate), CYAP (O,O-dimethyl-Op- cyanophenyl thiophosphate), DMTP (O,O-dimethyl-S[5-methoxy-1,3,4-thiadiazol-2(3H)onyl-(3)-methyl]dithiophosphate), BRP (dimethyl-1 ,2-dibromo-2,2-dichloroethyl phosphate), salichione (2-methoxy-4H-1,3,2-benzodioxaphosphorine-2-sulfide), DDVP (dimethyl 2,2-dichlorovinyl phosphate ), fenitrothion (MEP), (O,O-dimethyl-O-(3-methyl-4-nitrophenyl) thiophosphate), clothianidin ((E)-1-(2-chloro-1,3-thiazole-5 -ylmethyl)-3-methyl-2-nitroguanidine), imidacloprid (1-6-chloro-3-pyridylmethyl)-N-nitroimidazolidin-2-ylideneamine), acetamiprid ((E)-N ¹ -[(6- chloro-3-pyridyl)methyl]-N ² -cyano-N ¹ -methylacetamidine), thiamethoxam ((EZ)-3-(2-chloro-1,3-thiazol-1-ylmethyl)-5-methyl- 1,3,5-oxadiazinane-4-ylidene(nitro)amine), marathon (S-[1,2,-bis(ethoxycarbonyl)ethyl]dimethylphosphorothiolthionate), dimethoate (dimethyl S-(N- methylcarbamoylmethyl)dithiophosphate), PAP (S-[α-(ethoxycarbonyl)benzyl]dimethylphosphorothiolthionate), Fenthion (O,O-dimethyl-O-(3-methyl-4-methylthiophenylthiophosphate) )), BPMC (O-sec-butylphenylmethylcarbamate), MTMC (m-tolylmethylcarbamate), Meopal (3,4-dimethylphenyl-N-methylcarbamate), NAC (1-naphthyl-N-methylcarbamate) , Propoxur (2-isopropyloxyphenyl methylcarbamate), Methomyl (S-methyl-N[(methylcarbamoyl)oxy]thioacetimide), Cartap (1,3-bis(carbamoylthio)-2-(N,N-dimethylamino) ) propane hydrochloride) and the like, but are not limited thereto. These insecticidal components may be used in combination. Among them, boric acid-based compounds, dinotefuran, and fipronil are preferred, and boric acid-based compounds are more preferred.

The amount of the insecticidal component is such that after feeding, the insect can fly to the nest and exchange nutrients, and after the nutrition exchange, the eggs, larvae, imagoes immediately after emergence, etc. living in the nest die and the colony of the nest collapses. Prepared to an extent. Appropriate concentrations vary depending on the type of insecticidal component.
When the insecticidal component is a boric acid-based compound, the boric acid-based compound is 0.5% by mass to 15% by mass, preferably 0.5% by mass to 10% by mass, and 0.5% by mass with respect to the total amount of the bait composition. ~8% by mass, more preferably 0.5% to 6% by mass.
When the insecticidal component is dinotefuran, the amount of dinotefuran is 0.3 ppm to 100 ppm, preferably 0.3 ppm to 10 ppm, more preferably 0.3 ppm to 5 ppm, and more preferably 0.5 ppm to 5 ppm, based on the total amount of the bait composition.
When the insecticidal component is fipronil, the amount of fipronil in the bait composition is 0.1 ppm to 100 ppm, preferably 0.1 ppm to 20 ppm, more preferably 0.1 ppm to 15 ppm, more preferably 0.1 ppm to 10 ppm.
The details of the compounding amount will be described later.

(Formulation of bait composition)
A bait composition is food for Waspidae insects. That is, in addition to the insecticidal component, it is common to add components contained in sap, nectar, fruit trees, etc., and solid substances that serve as food for larvae, such as living organisms (insects, spiders, grasshoppers, crickets, etc.). ), meat, fish, cat food, dog food, and insect jelly.

(attractive component)
The bait composition is preferably installed near the feeding area of the Waspidae insects, but the bait composition may further contain an attracting component in order to attract the Waspidae insects to the installation site of the bait composition.
Examples of the attractant include dairy products such as fermented milk, liquids such as vinegar, sugars, fragrances, essential oils, alcohols, and fruit juices. These may be used singly or in combination of two or more. Furthermore, sap, nectar, ingredients contained in fruit trees, etc. may be blended, and solids of ingredients that serve as food for larvae, such as living organisms (insects, spiders, grasshoppers, crickets, etc.), meat, fish, cat food. , dog food, or insect jelly may be added as an attractant.

(Other ingredients)
The bait composition may contain preservatives, antioxidants, solvents, antifreeze agents, antifoaming agents, etc., which are used in the field of insecticides, in addition to insecticidal components and attracting components. Examples of antiseptic agents include Bestside 750 (manufactured by Nippon Soda Co., Ltd.). Further, in order to make all or part of the bait composition gel or solid, a gelling agent such as agar, guar gum, sodium polyacrylate, or the like, a thickening agent, or the like may be mixed. Furthermore, a bittering agent or the like may be blended to prevent accidental ingestion by humans. Vitrex etc. are illustrated as a bittering agent.

(control container)
A container for controlling vespidae insects of the present invention comprises the above bait composition.
The shape of the control container is not particularly limited as long as it has a structure that allows the vespidae insects to eat the bait composition, does not drown in the bait composition, can retreat from food, and can fly.
When the bait composition is liquid or gel with high fluidity, it is desirable that the scaffold be located at a position where the bait composition can be pecked with the beak. For example, the bait composition can be placed in a control container such as a cup.
In addition, when the bait composition is set in a control container that is a cup with a certain depth in order to be less susceptible to wind and rain and to be less likely to dry, wasp insects descend near the liquid surface in the cup, It is preferable to provide a scaffold as appropriate so that the animal can exit after eating.

In addition, when the bait composition is solid or in the form of a gel with low fluidity, a small piece of the bait composition is set on a flat surface through which the Waspidae insects can pass, and the Waspidae insects can freely enter and exit the control container. may be
Furthermore, when the bait composition is in the form of a liquid or a highly fluid gel, a bait composition holder such as paper or absorbent cotton is impregnated with the bait composition to form a flat surface through which the Wasps can pass. It may be a control container on which a bait composition holder impregnated with a bait composition is set and into which the Waspidae insects can freely enter and exit.

(Control method)
In the method for controlling Waspidae insects of the present invention, at least an insecticidal component is blended to prepare a bait composition; is set, and the control container is installed at a predetermined distance from the nest of Waspidae insects.
That is, the Wasp insects fly to the place where the bait composition is installed, feed on the bait composition containing the insecticidal component, then leave and fly back to the nest, and the adults and nutrition in the nest. Configured to replace, die, and collapse nest colonies.

The insecticidal component and its compounding amount in the control method are the same as those described above for the bait composition. Since the solubility differs depending on the insecticidal component, it may be preferable to change the solvent depending on the concentration.
The bait composition may also contain an attractant component. The attractant component is the same as described above for the bait composition.
Additionally, the bait composition may contain other ingredients. Other components are the same as those described above for the bait composition.

The above bait composition is set in a control container having a structure that allows vespidae insects to eat, leave after feeding, and fly.
The control container in the control method is the same as the control container described above.

The control container is placed at a predetermined distance from the nest of Waspidae insects. The installation location is appropriately set in consideration of topography, tree conditions, flight conditions of Waspidae insects, the effects of wind and rain, the effects of other natural enemies, the proximity of feeding grounds, and the like. As a result, the predetermined distance from the nest to the control container is often about 0.5m to 20m, or 1m to 10m, or 1m to 5m. In addition, it is preferable to set the height above the ground in the range of 0.5 m to 4.0 m, more preferably in the range of 0.5 m to 3.0 m, so that the wasps can easily fly and eat. preferable.

- Concentration study test of insecticidal component 1 -
When disodium octaborate tetrahydrate (Timboa/manufactured by US Borax/hereinafter referred to as Timboa) among boric acid compounds is selected as an insecticidal component, the concentration suitable for controlling Waspidae insects is examined. Therefore, the following tests were conducted.

As shown in Table 1, bait compositions with varying concentrations of Timboa were prepared.

Absorbent cotton was placed in a glass Petri dish (3 cm in diameter, without a lid), and 8 ml of each sample was soaked therein. This was placed in the corner of the test container (plastic cage, 18x9x10 cm). One fasted adult wasp was subjected to low-temperature anesthesia, placed in a test container, and the presence or absence of death was confirmed over time to investigate the cumulative mortality rate. Cumulative mortality is the proportion of individuals exhibiting behavioral arrest. The start of the test was immediately after the adult wasp ingested the sample.
Concentration study test 1 was performed three times for each of samples 1 to 8.
Table 2 shows changes in cumulative mortality in each sample.

As shown in Table 2, none of samples 1 to 8 died until 5 hours after ingestion. In addition, sample 1 (6.0% by mass of Timboa) was 10 hours after ingestion, sample 2 (4.0% by mass of Timboa) was 24 hours after ingestion, and sample 3 (2.0% by mass of Timboa) was 15 hours after ingestion. Cumulative mortality reached 100%. Sample 4 (1.0% by weight of Timboa) and Sample 5 (0.5% by weight of Timboa) did not reach 100% cumulative mortality at 46 hours post-feeding when tested, whereas sample 4 was at 21 hours post-feeding. In sample 5, death of some adult scarlet wasps was confirmed 46 hours after ingestion.

The Waspidae insect bait composition of the present invention allows the Waspidae insect to return to the nest after eating the bait composition, exchange nutrients with larvae and adults in and near the nest, and the insecticidal component is It is required that the composition is such that individuals, larvae, and adults who ingest the so-called poisonous bait contained will die. Here, since the bait collection time for Waspidae insects is about 5 to 20 minutes, it is considered that an individual that can act (or fly) for about 30 minutes after ingesting the bait composition will suffice. be done. Therefore, the upper limit of the concentration of the bait composition may be set within a range that does not cause death for 30 minutes after ingestion (diet).
On the other hand, the lower limit of the concentration of the bait composition is the possibility that the individuals who finally ingested the poisoned bait, the larvae who underwent nutrition exchange, the adults, and the larvae who were not given food after hatching from the eggs died, and the colony of the nest collapsed. is set to some concentration.

From the results of concentration study test 1, when the insecticidal component is timboa, which is a boric acid compound, the upper limit of the concentration is 15% by mass, preferably 10% by mass, 8% by mass, more preferably 6% by mass, relative to the total amount of the bait composition. It was found that it is possible to take action for about 30 minutes after ingestion by setting it to mass %. On the other hand, it was found that the lower limit of the concentration should be set to 0.5% by mass, preferably 1.0% by mass, based on the total amount of the bait composition.
Therefore, when the insecticidal component is a boric acid compound, Timboa is 0.5% by mass to 15% by mass, preferably 0.5% by mass to 10% by mass, and 0.5% by mass to 8 mass %, more preferably 0.5 mass % to 6 mass %.

-Concentration study test of insecticidal component 2-
When dinotefuran ((RS)-1-methyl-2-nitro-3-(tetrahydro-3-furylmethyl)guanidine) is selected as the insecticidal component, the following is used to examine the concentration suitable for controlling Wasp insects. did the test.

As shown in Table 3, bait compositions with different concentrations of dinotefuran were prepared.

Absorbent cotton was placed in a glass Petri dish (3 cm in diameter, without a lid), and 8 ml of each sample was soaked therein. This was placed in the corner of the test container (plastic cage, 18x9x10 cm). Three fasted adult wasps were subjected to cold anesthesia, placed in a test container, and the presence or absence of death was confirmed over time to investigate the cumulative mortality rate. Cumulative mortality is the proportion of individuals exhibiting behavioral arrest. The start of the test was immediately after the adult wasp ingested the sample.
Concentration study test 2 was performed three times for each of samples 9-18.
Table 4 shows changes in cumulative mortality in each sample.

As shown in Table 4, none of samples 12 to 18 died until 30 minutes after ingestion. Sample 11 (dinotefuran 10.0 ppm) was confirmed to have a cumulative mortality rate of 22.2% 30 minutes after ingestion, and some wasps died, but more than half of the wasps did not stop their behavior. Sample 10 (dinotefuran 50.0 ppm) and sample 9 (dinotefuran 100.0 ppm) were confirmed to have a cumulative mortality rate of 66.7% at 30 minutes after ingestion, while 33.3% were confirmed not to stop behavior. .
Samples 12-14 (0.5 ppm to 5.0 ppm dinotefuran) resulted in 100% cumulative mortality by 20 hours after feeding. Sample 15 (dinotefuran 0.3 ppm) did not reach a cumulative mortality rate of 100% by 30 hours after ingestion of the test, but more than half of the adult wasps were confirmed dead 24 hours after ingestion, and insecticidal activity was demonstrated. found to have. In samples 16-18 (0 ppm to 0.2 ppm dinotefuran), no mortality was observed 30 hours after ingestion when the test was performed.

From the results of concentration study test 2, it was found that the upper limit of the concentration when the insecticidal component is dinotefuran should be set to 100 ppm, preferably 10 ppm or 5 ppm, relative to the total amount of the bait composition. On the other hand, it was found that the lower limit of the concentration should be set to 0.3 ppm, preferably 0.5 ppm with respect to the total amount of the bait composition.
Therefore, when the insecticidal component is dinotefuran, the amount of dinotefuran is 0.3 ppm to 100 ppm, preferably 0.3 ppm to 10 ppm, more preferably 0.3 ppm to 5 ppm, and more preferably 0.5 ppm to 5 ppm, relative to the total amount of the bait composition.

-Concentration study test of insecticidal component 3-
When fipronil (5-amino-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-4-trifluoromethylsulfinyl)pyrazole-3-carbonitrile) is selected as an insecticidal component, wasp insects The following test was conducted in order to examine the concentration suitable for the control of .

As shown in Table 5, bait compositions with different concentrations of fipronil were prepared. Since fipronil has low solubility in water, a dissolution aid was added.

Absorbent cotton was placed in a glass Petri dish (3 cm in diameter, without a lid), and 8 ml of each sample was soaked therein. This was placed in the corner of the test container (plastic cage, 18x9x10 cm). One fasted adult wasp was subjected to low-temperature anesthesia, placed in a test container, and the presence or absence of death was confirmed over time to investigate the cumulative mortality rate. Cumulative mortality is the proportion of individuals exhibiting behavioral arrest. The start of the test was immediately after the adult wasp ingested the sample.
Concentration study test 3 was performed three times for each of samples 19-24.
Table 6 shows changes in cumulative mortality in each sample.

As shown in Table 6, none of the samples 19 to 24 died not only within 30 minutes after ingestion, which is the standard, but also within 3 hours after ingestion. Sample 19 (10.0 ppm fipronil) was 5 hours after ingestion, sample 20 (1.0 ppm fipronil) was 19 hours after ingestion, sample 21 (0.2 ppm fipronil) and sample 22 (0.1 ppm fipronil) were after ingestion. Cumulative mortality reached 100% at 21 hours.
Sample 23 (fipronil 0.05 ppm) did not show a cumulative mortality of 100% 46 hours after ingestion when the test was performed, but death of some adult wasps was confirmed 21 hours after ingestion. Moreover, sample 24 (fipronil 0 ppm), which is a blank, also showed the same cumulative mortality rate as sample 23, suggesting that the solubilizing agent also affects the cumulative mortality rate.

From the results of concentration study test 3, when the insecticidal component is fipronil, the upper limit of the concentration may be set to 100 ppm, preferably 20 ppm or 10 ppm, with respect to the total amount of the bait composition, and it is possible to act for about 30 minutes after ingestion. I found out. On the other hand, it was found that the lower limit of the concentration should be set to 0.1 ppm with respect to the total amount of the bait composition.
Therefore, when the insecticidal component is fipronil, the amount of fipronil in the bait composition is 0.1 ppm to 100 ppm, preferably 0.1 ppm to 20 ppm, more preferably 0.1 ppm to 15 ppm, more preferably 0.1 ppm to 10 ppm.

-Evaluation test 1-
When 5.0 ppm of dinotefuran was blended as an insecticidal component in the bait composition for wasps of the present invention, the effect of exterminating the nests of wasps was confirmed.

A bait composition containing 0.0001% of a bittering agent (Bitrex) was used as sample 12, and the bait composition was set in the control container shown in FIGS. 1(a) and 1(b).
The control container used in the evaluation test had an opening diameter of 93 mm and a depth of 110 mm, a hole with a diameter of 3.2 cm was made in the center of the lid, and a netron protector C-16 black with an outer diameter of 3 cm (Dainippon Plastics Co., Ltd.) manufactured by the company, hereinafter referred to as "net tube") was cut to a length of 10 cm and passed through the hole of the plastic cup lid (see Fig. 1(b)). 250 ml of sample 12 containing a bittering agent was poured into the inside of the plastic cup, and a cover was assembled to protect against wind and rain (see Fig. 1(a)). The color of the lid and main body of the control container used in the evaluation test was yellow.

Five control containers provided with the bait composition of Sample 12 containing a bittering agent were placed in a range of 2 m to 20 m for three hornet nests to be exterminated.
Nest diameter and height above the ground and the distance between each nest and control container are shown in Table 7. Table 8 shows the height of each control container from the ground. In addition, a top schematic view of the location of nests 1-3 and control containers A-E with bait compositions is shown in FIG.

As shown in FIG. 2, for each nest, one control container containing the bait composition was placed at a minimum distance of 2 m to 3.5 m from each nest. Some of the control containers installed for each nest are also at a distance of about 3.5m to 5.5m from other nests. Furthermore, control containers D and E were placed near so-called feeding grounds where many Waspidae insects fly.
In addition, the control container was installed at a height of 1.5 m to 2.5 m above the ground so that the Waspidae insects can easily fly and eat.

Before the start of the evaluation test, it was confirmed that the colonies of nests 1 to 3 were active. We counted the number of hornets that entered and exited the nest by stimulating it. In addition, it was estimated that about 50 to 100 hornets live in one nest.

One week after the installation of the control container with the bait composition, the nest was stimulated to check for the entry and exit of the hornets. When no individuals entered and exited, the nest was dismantled and the presence or absence of living insects was confirmed. When there were individuals coming and going, the evaluation was continued every other week, and when there were no more individuals coming and going, the nest was dismantled and the presence or absence of living insects was confirmed.
In weekly follow-up observations, in addition to confirming the presence or absence of the entry and exit of the hornet when the nest was stimulated as described above, confirmation of dead individuals around the nest, confirmation of the state of the nest during dismantling of the nest, and further The amount of decrease in the bait composition in the control container was confirmed.
Based on a comprehensive judgment of the confirmation results described above, it was determined whether or not the bait composition caused colony collapse.
Table 9 shows the evaluation results of Evaluation Test 1. Also, Table 10 shows the amount of decrease in the bait composition.
3(a) shows the state of nest 1 at the start of the test, FIG. 3(b) shows the state of nest 2 at the start of the test, and FIG. 3(c) shows the state of nest 3 at the start of the test. In addition, the state of nest 1 dismantled two weeks after installation in FIG. 4(a), the state of nest 2 dismantled two weeks after installation in FIG. 4(b), and the dismantling two weeks after installation in FIG. 4(c) The situation of Nest 2 is shown.

As shown in Tables 9 and 10, Figures 3 and 4, in Nest 1 two weeks after installation of the control container, bee activity around the nest could not be confirmed, and a large number of dead larvae were confirmed in the nest. Also, the bait composition set in the control container A placed around nest 1 showed a decrease between one week and two weeks after placement. From the above results, it was found that nest 1 was affected by the bait composition and colony collapse occurred.

Two weeks after installation of the control container in nest 2, bee activity around the nest could not be confirmed, and a large number of dead larvae were confirmed in the nest. In addition, although there was no decrease in the bait composition set in the control container B installed at a distance of 3.5 m from the nest 2, it was set in the control container C installed at a distance of 5.5 m from the nest 2. The bait composition was greatly reduced between one week and two weeks after installation. From the above results, it was found that nest 2 was affected by the bait composition and colony collapse occurred. Surviving larvae and eggs were also found in the dismantled nest, suggesting that the colony had just collapsed.

On the other hand, for nest 3, a decrease in the number of bees active around the nest was confirmed 3 weeks after installation of the control container. However, three to four weeks after installation, the nest 3 was directly hit by a typhoon and collapsed, and the nest 3 was not evaluated.

-Evaluation test 2-
As an insecticidal component of the vespid insect bait composition of the present invention, when 5.0 ppm of dinotefuran is blended, the effect of exterminating the nest of the hornet wasp is confirmed, and an analysis of whether dinotefuran is contained in the dead individual of the adult wasps. gone.

A bait composition containing 0.0001% of a bittering agent (Bitrex) was used as sample 12, and the bait composition was set in the control container shown in FIGS. 1(a) and 1(b).
The control container used in the evaluation test had an opening diameter of 93 mm and a depth of 110 mm, a hole with a diameter of 3.2 cm was made in the center of the lid, and a netron protector C-16 black with an outer diameter of 3 cm (Dainippon Plastics Co., Ltd.) (manufactured by the company) was cut to a length of 10 cm and passed through the hole of the plastic cup lid (see Fig. 1(b)). 250 ml of sample 12 containing a bittering agent was poured into the inside of the plastic cup, and a cover was assembled to protect against wind and rain (see Fig. 1(a)). The color of the lid and main body of the control container used in the evaluation test was yellow.

Three control containers (control containers F, G, H )installed.
Nest diameter and height above the ground and the distance between each nest and control container are shown in Table 11. Table 12 shows the height of each control container from the ground. In addition, a top schematic view of the location of nests 4-6 and control containers FH with bait compositions is shown in FIG.

As shown in FIG. 5, one pest control container containing the bait composition was installed at a minimum distance of 5.0 m to 7.0 m for each nest. In addition, the control container G installed with respect to the nest 5 is at a distance of about 10.0 m from the nest 6 .
In addition, the control container was installed at a height of 1.5 m to 3.0 m above the ground so that the Wasp family insects can easily reach and eat by flying.

Before the start of the evaluation test, it was confirmed that the colonies of nests 4 to 6 were active. We counted the number of hornets that entered and exited the nest by stimulating it. In addition, it was estimated that about 50 to 100 hornets live in one nest.

One week after the installation of the control container with the bait composition, the nest was stimulated to check for the entry and exit of the hornets. When no individuals entered and exited the nest, the nest was dismantled and the presence or absence of living insects was confirmed. When there were individuals coming and going, the evaluation was continued every other week, and when there were no more individuals coming and going, the nest was dismantled and the presence or absence of living insects was confirmed.
In weekly follow-up observations, in addition to confirming the presence or absence of the entry and exit of the hornet when the nest was stimulated as described above, confirmation of dead individuals around the nest, confirmation of the state of the nest during dismantling of the nest, and further A decrease in the bait composition in the control container was confirmed.
Based on a comprehensive judgment of the confirmation results described above, it was determined whether or not the bait composition caused colony collapse.
Table 13 shows the evaluation results of Evaluation Test 2. Also, Table 14 shows the amount of decrease in the bait composition.
Further, FIG. 6(a) shows the state of nest 4 at the start of the test, FIG. 6(b) shows the state of nest 5 at the start of the test, and FIG. 6(c) shows the state of nest 6 at the start of the test. In addition, FIG. 7(a) shows the state of the inside of the nest 4 three weeks after installation, and FIG. , Fig. 7(c) shows the situation of dead larvae in nest 4 three weeks after installation, Fig. 8(a) shows the situation in nest 6 three weeks after installation, Fig. 8(b) shows the situation in nest 3 weeks after installation Fig. 8(c) shows the state of the dead adult individuals in Nest 6, and the state in Nest 5 4 weeks after installation.

From Tables 13 and 14 and FIGS. 6 to 8, in Nest 4, one week after installation of the control container, the number of bees around the nest decreased, and a large number of dead adult bees existed just below the nest. After 3 weeks from installation, bee activity around the nest could not be confirmed, and many dead larvae were confirmed within the nest. Among the dead larvae, there were also confirmed dead individuals in the state of whitening (become a clearly abnormal individual with dehydrated state). Surviving larvae just before emergence were confirmed in the nest, and evidence of nest building activity was confirmed until just before the colony collapsed. The bait composition set in the control container F installed around the nest 4 decreased after one week of installation. From the above results, it was found that nest 4 was affected by the bait composition and colony collapse occurred.

In nest 5, the number of bees active around the nest decreased 3 weeks after installation of the control container, and the activity of bees around the nest could not be confirmed 4 weeks after installation. Many dead larvae were found in the nest. In addition, surviving larvae and eggs just before emergence were also confirmed, and evidence of nesting activity was confirmed until just before the collapse of the colony. In Nest 5, although it was difficult to fly, adults were confirmed to be alive. The bait composition set in the control container G placed at a distance of 5.0 m from the nest 5 also decreased after 3 weeks of installation. From the above results, it was found that nest 5 was affected by the bait composition and colony collapse occurred.

In nest 6, the number of bees active around the nest decreased one week after installation of the control container, and the activity of bees around the nest could not be confirmed three weeks after installation. In the nest, dead larvae immediately before emergence were confirmed, and about 40 dead adults were also confirmed. Surviving larvae and eggs just before emergence were also confirmed, and evidence of nest building activity was confirmed until just before the colony collapsed. It was also confirmed that the bait composition set in the control container H placed at a distance of 5.0 m from the nest 6 decreased two weeks after installation. From the above results, it was found that nest 6 was affected by the bait composition and colony collapse occurred.

In addition, the dead larvae that were bleached (obviously dehydrated and became abnormal individuals) in the nest observed in nest 4, and the large number of dead adults observed in nests 4 to 6 were observed under natural conditions. It is known that this is not possible.
In Nest 5, bee activity around the nest could not be confirmed, and a decrease in the bait composition was observed. This indicates that the pupae that pupated before the control container with the bait composition was installed did not undergo nutrient exchange, had no insecticidal effect, and were likely to emerge normally.

Whether dinotefuran was contained in a large number of dead adults confirmed in Nest 6 was analyzed by liquid chromatography-mass spectrometry.
The analysis results are shown in Table 15.

As shown in Table 15, as a result of analyzing a large number of dead adults confirmed in Nest 6, 0.001 μg/animal (0.07 ppm) of dinotefuran was detected per dead adult.
That is, it was confirmed that the insecticidal component contained in the bait composition was incorporated into the colony collapse of nest 6 by wasps.

-Evaluation test 3-
The bait composition for Waspidae insects of the present invention, when 10 ppm of fipronil was added as an insecticidal component, was confirmed to be effective in exterminating the nests of the hornet and the yellow wasp.

Sample 19 was used as the bait composition, and the bait composition was set in the control container shown in FIGS. 1(a) and 1(b).
The control container used in the evaluation test had an opening diameter of 93 mm and a depth of 110 mm, a hole with a diameter of 3.2 cm was made in the center of the lid, and a netron protector C-16 black with an outer diameter of 3 cm (Dainippon Plastics Co., Ltd.) (manufactured by the company) was cut to a length of 10 cm and passed through the hole of the plastic cup lid (see Fig. 1(b)). 250 ml of the sample 12 was poured into the inside of the plastic cup, and a cover serving as a wind and rain shelter was assembled (see FIG. 1(a)). The color of the lid and main body of the control container used in the evaluation test was yellow.

Three control containers (control containers I and J) containing the bait composition of sample 19 were installed within a range of 5 m for each one nest of the hornet or the hornet to be exterminated (nests 7 to 8).
The nest diameter and height above the ground, the height of the control container above the ground, and the distance between each nest and the control container are shown in Table 16.

For each nest, a control container with the bait composition was placed at a distance of 5 m, as shown in Table 16. In addition, the control container was installed at a height of 1.5 m above the ground so that the Waspidae insects can easily fly and arrive and eat easily. In addition, mutual nests were installed at intervals of about 15 m.

Before the start of the evaluation test, it was confirmed that the colonies of both nests 7 and 8 were active. It was estimated that about 50 to 100 wasps live in one nest by counting the number of hornets or yellow hornets that enter and leave the nest by stimulating them.

One week after the installation of the control container with the bait composition, the nest was stimulated to confirm the presence or absence of entry and exit of the hornet or the hornet. When no individuals entered and exited, the nest was dismantled and the presence or absence of living insects was confirmed. When there were individuals coming and going, the evaluation was continued every other week, and when there were no more individuals coming and going, the nest was dismantled and the presence or absence of living insects was confirmed.
In weekly follow-up observations, in addition to confirming the presence or absence of entry and exit of the hornet or yellow hornet when the nest is stimulated as described above, confirmation of dead individuals around the nest and confirmation of the state of the nest during dismantling of the nest. Furthermore, the reduction amount of the bait composition in the control container was confirmed.
Based on a comprehensive judgment of the confirmation results described above, it was determined whether or not the bait composition caused colony collapse.
Table 17 shows the evaluation results of Evaluation Test 3. In addition, Table 18 shows the amount of decrease in the bait composition.
9(a) and 9(b) show the state of the nest 8 when dismantled.

From Tables 17 and 18 and FIG. 9, it was confirmed that the number of bees active around the nest 7 decreased one week after installation of the control container, and the activity status also decreased. Two weeks after installation, no bees were active around the nest, so the nest was dismantled and investigated. There were many dead larvae in the nest. In addition, since the amount of the bait composition provided in the control container I decreased during the evaluation test period, it is considered that the colony collapsed due to the influence of the bait composition.

Nest 8 confirmed a tendency for the number of bees active around the nest to decrease one week after installation of the control container. A tendency to decline was also observed in activity status. Two weeks after installation, no bees were found to be active around the nest, so the nest was dismantled and investigated. There were many dead larvae and about 10 dead adults in the nest. A decrease in the bait composition in the control container J was also confirmed during the evaluation test. When the content of fipronil in dead adults was analyzed by liquid chromatography-mass spectrometry, 0.014 ppm of fipronil was detected. From these results, it is considered that the collapse of colonies is caused by the influence of the bait composition.

As described above, from the results of Evaluation Tests 1 to 3, by installing a control container equipped with a bait composition having the composition of the present invention, Waspidae insects eat the bait composition, return to the nest, and larvae through nutrient exchange. and adults can be given insecticidal ingredients, causing them to die and eventually collapse the nest colony.

-Concentration study test of insecticidal component 4-
When boric acid (orthoboric acid) is selected from among boric acid compounds as an insecticidal component, the following test was conducted in order to examine the concentration suitable for controlling Waspidae insects.

As shown in Table 19, bait compositions with different concentrations of boric acid were prepared.

Absorbent cotton was placed in a glass petri dish (3 cm in diameter, without a lid), and 8 ml of sample 1 was soaked into the petri dish. This was placed in the corner of the test container (plastic cage, 18x9x10 cm). One fasted adult wasp was subjected to low-temperature anesthesia, placed in a test container, and the presence or absence of death was confirmed over time to investigate the cumulative mortality rate. Cumulative mortality is the proportion of individuals exhibiting behavioral arrest. The start of the test was immediately after the adult wasp ingested the sample.
Concentration study test 4 was conducted three times for each of samples 25-27.
Table 20 shows changes in cumulative mortality in each sample.

As shown in Table 20, none of samples 25 to 27 died until 6 hours after ingestion. In addition, sample 25 (1.0% by mass of boric acid) had a cumulative mortality rate of 100% 26 hours after ingestion. Sample 26 (0.5% by mass of boric acid) did not show a cumulative mortality rate of 100% 46 hours after ingestion, but death of some adult wasps was confirmed 37 hours after ingestion.

The Waspidae insect bait composition of the present invention allows the Waspidae insect to return to the nest after eating the bait composition, exchange nutrients with larvae and adults in and near the nest, and the insecticidal component is It is required that the composition is such that individuals, larvae, and adults who ingest the so-called poisonous bait contained will die. Here, since the bait collection time for Waspidae insects is about 5 to 20 minutes, it is considered that an individual that can act (or fly) for about 30 minutes after ingesting the bait composition will suffice. be done. Therefore, the upper limit of the concentration of the bait composition may be set within a range that does not cause death for 30 minutes after ingestion (diet).
On the other hand, the lower limit of the concentration of the bait composition is the possibility that the individuals who finally ingested the poisoned bait, the larvae who underwent nutrition exchange, the adults, and the larvae who were not given food after hatching from the eggs died, and the colony of the nest collapsed. is set to some concentration.

The results of the concentration study test 4 of the insecticidal component are combined with the results of the concentration study test 1 of the boric acid-based compound described above, and when the insecticidal component is boric acid among the boric acid-based compounds, the upper limit of the concentration is the bait composition. 15% by mass, preferably 10% by mass, 8% by mass, more preferably 6% by mass, of the total amount, and it was found that it was possible to act for about 30 minutes after ingestion. On the other hand, it was found that the lower limit of the concentration should be set to 0.5% by mass, preferably 1.0% by mass, based on the total amount of the bait composition.
Therefore, when the insecticidal component is a boric acid compound, boric acid is 0.5% by mass to 15% by mass, preferably 0.5% by mass to 10% by mass, and 0.5% by mass with respect to the total amount of the bait composition. ~8% by mass, more preferably 0.5% to 6% by mass.

Claims (5)

A bait composition containing an insecticidal component of Waspidae insects,
The insecticidal component contains any one of a boric acid compound, dinotefuran, and fipronil as an active ingredient,
When the insecticidal component is a boric acid-based compound, the boric acid-based compound is 0.5% by mass to 15% by mass with respect to the total amount of the bait composition,
When the insecticidal component is dinotefuran, dinotefuran is 0.3 ppm to 100 ppm with respect to the total amount of the bait composition,
A bait composition wherein, when the insecticidal component is fipronil, fipronil is 0.1 ppm to 100 ppm relative to the total amount of the bait composition. 2. The bait composition of claim 1, wherein the bait composition further comprises an attractant component for Waspidae insects. 3. A container for controlling wasp family insects, comprising the bait composition according to claim 1 or 2, and having a structure in which the wasp family insects can eat the bait composition and can exit after eating. A bait composition is prepared by blending an insecticidal component,
setting the bait composition in a control container having a structure in which the Waspidae insects can eat and can exit after feeding;
A method for controlling Waspidae insects, wherein the control container is placed at a predetermined distance from the nest of Waspidae insects,
The insecticidal component contains any one of a boric acid compound, dinotefuran, and fipronil as an active ingredient,
When the insecticidal component is a boric acid-based compound, the boric acid-based compound is 0.5% by mass to 15% by mass with respect to the total amount of the bait composition,
When the insecticidal component is dinotefuran, dinotefuran is 0.3 ppm to 100 ppm with respect to the total amount of the bait composition,
A control method, wherein when the insecticidal component is fipronil, fipronil is 0.1 ppm to 100 ppm with respect to the total amount of the bait composition. 5. The control method according to claim 4, wherein the bait composition further contains a component that attracts Waspidae insects.

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