JP2023036560A5 - - Google Patents

Claims (23)

An additive for proteins or cells, which comprises an amino acid-based polymer (A) having a structural unit containing an amino acid residue. The additive according to claim 1, wherein the amino acid polymer (A) has a mass average molecular weight of 2,000 or more. 3. The amount of antifreeze water measured in an aqueous solution in which the amino acid polymer (A) has a concentration of 20.0 w/v% is 200 mg or more per 1 g of the polymer (A). Additive. The additive according to claim 1 or 2, wherein the amino acid-based polymer (A) has at least one of the structural units represented by the following general formulas (1) to (3).

...General formula (1)

...General formula (2)

...General formula (3)
(In general formulas (1) to (3), A is an amino acid residue, X and Y are each independently O or NH, R1 is an alkylene group, R2 is an alkylene group, and one or more hydrogen atoms are hydroxyl groups. Indicates a nonionic divalent group consisting of a substituted alkylene group, phenylene group, ester group, ether group, urethane bond, amide bond, or a combination thereof.) The amino acid-based polymer (A) contains at least 30 structural units represented by general formulas (1) to (3) based on 100% by mass of the structural units constituting the amino acid-based polymer (A). The additive according to claim 1 or 2, containing ~100% by mass. A cell culture medium comprising the additive according to claim 1 or 2. The cell culture medium according to claim 6, wherein the concentration of the amino acid polymer (A) in the medium is 0.001 to 1% by mass. A method for producing spheroids or physiologically active substances, which comprises culturing cells in the cell culture medium according to claim 6. The additive according to claim 1 or 2, which is a protein stabilizer or a blocking agent. The additive according to claim 9, wherein the concentration of the amino acid polymer (A) is 0.01 to 30.0 w/v %. A method for stabilizing a protein, comprising coexisting the additive according to claim 9 with the protein. An immunoassay method comprising the step of treating a carrier containing an antigen or an antibody with the additive according to claim 9. The immunoassay method according to claim 12, wherein the carrier is latex particles. A carrier comprising an antigen or an antibody against a target substance, the carrier being partially or completely coated with the additive according to claim 9. A kit for immunoassay comprising the carrier according to claim 14. The additive according to claim 1 or 2, which is a cell cryoprotectant. A cell cryopreservation solution comprising the additive according to claim 16. The cell cryopreservation solution according to claim 17, further comprising a low molecular weight solvent and having an osmotic pressure of 500 to 2,500 mOsm/kg. The cell cryopreservation solution according to claim 17, wherein the concentration of the amino acid-based polymer (A) is 0.1 to 30.0 w/v%. 19. The cell according to claim 18, wherein the low molecular solvent is at least one selected from the group consisting of dimethyl sulfoxide, propylene glycol, ethylene glycol, and glycerin, and the content of the low molecular solvent is 5.0 w/v% or less. Cryopreservation solution. A method for freezing cells, comprising the steps of: mixing the cell cryopreservation solution according to claim 17 with cells; and freezing a mixture of the cell cryopreservation solution and cells. A spheroid frozen using the cell cryopreservation solution according to claim 17. An organoid frozen using the cell cryopreservation solution according to claim 17.