JP2023020633A - Fluorescence measuring device - Google Patents

Abstract

To provide a fluorescence measuring device having a simple configuration.SOLUTION: A fluorescence measuring device measures concentration of a measurement object under measured water, and includes a measurement room for storing the measured water, a light projection path for projecting light in a first direction toward the measurement room, a measurement optical path for emitting light in a direction differing from the first direction from the measurement room, a transmission optical path for emitting light onto an extension line of the light projection path from the measurement room, a first light source for projecting first measurement light having a first wavelength for exciting the measurement object in the measurement room through the light projection path and generating fluorescence, a second light source for projecting second measurement light having a second wavelength that is identical or approximate to a wavelength of the fluorescence of the measurement object into the measurement room through the light projection path, a first light receiving element for receiving light emitted from the measurement optical path, a second light receiving element for receiving light emitted from the transmission optical path, and a light quantity adjustment mechanism for adjusting an amount of light projection from at least one of the first light source and the second light source to the measurement room.SELECTED DRAWING: Figure 1

Description

The present invention relates to a fluorometer.

As a method for checking the water quality, measurement light with a wavelength that excites the measurement target to cause fluorescence is projected onto the water to be measured, and the fluorescence of the measurement target is measured by a light receiving element provided at a position where the measurement light does not directly enter. Therefore, a method of calculating the concentration of the object to be measured is known. If the water to be measured contains a component that scatters or absorbs light, such as turbidity, in addition to the object to be measured, the amount of light received by the light receiving element is affected by these components. For this reason, light with a wavelength that does not excite the measurement target is projected to measure transmitted light and scattered light, and based on these measured values, scattering of light by components other than the measurement target that can be included in the fluorescence measurement value and an apparatus for correcting errors due to absorption has been proposed (see, for example, Patent Document 1).

Japanese Patent No. 6436266

In the fluorescence measurement apparatus described in Patent Document 1, a light source for fluorescence measurement and a light source for scattered light measurement are coaxially arranged in water to be measured so as to be capable of projecting light, and fluorescence is measured at an angular position of 90° with respect to this light projection position. , a second detector for detecting transmitted light at an angular position of 180°, and a third detector for detecting scattered light at an angular position of 270°. be.

SUMMARY OF THE INVENTION Accordingly, it is an object of the present invention to provide a fluorescence measurement apparatus with a simple configuration.

A fluorescence measurement device according to an aspect of the present invention is a fluorescence measurement device that measures the concentration of a measurement target in water to be measured, comprising: a measurement chamber storing the water to be measured; a measurement optical path through which light is emitted from the measurement chamber in a direction different from the first direction; a transmission optical path through which light is emitted from the measurement chamber on an extension line of the projection path; and the projection path a first light source for projecting a first measurement light having a first wavelength that excites the measurement object to cause fluorescence into the measurement chamber through a first light source, and a wavelength of the fluorescence of the measurement object that is projected onto the measurement chamber through the light projection path; A second light source for projecting a second measurement light having the same or similar second wavelength, a first light receiving element for receiving light emitted from the measurement optical path, and a second light receiving element for receiving light emitted from the transmission optical path. and a light quantity adjusting mechanism for adjusting the quantity of light projected from at least one of the first light source and the second light source to the measurement chamber.

In the fluorescence measurement device described above, the light amount adjustment mechanism may have a diaphragm that restricts the optical path of at least one of the first light source and the second light source.

In the fluorescence measurement device described above, the first light source and the second light source may be arranged adjacently on the same substrate.

The fluorescence measurement device described above may further include a filter that removes the first wavelength component from the light emitted from the measurement optical path.

The above-described fluorescence measurement apparatus includes a lid portion that can be opened and closed in the measurement chamber, and an open determination that determines that the lid portion is in an open state when the first light receiving element detects light equal to or greater than a predetermined threshold. You may further comprise a part.

The above-described fluorescence measurement apparatus includes a cover portion that can be opened and closed in the measurement chamber, and the first light receiving element or the second light receiving element that emits light when the first measurement light and the second measurement light are not projected. and an open determination unit that determines that the lid is in the open state when the is detected.

According to the present invention, it is possible to provide a fluorescence measurement device with a simple configuration.

1 is a schematic diagram showing the configuration of a fluorescence measurement device according to an embodiment of the present invention; FIG. 2 is a flow chart showing a procedure of concentration measurement by the fluorometer of FIG. 1;

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, embodiments of the present invention will be described with reference to the drawings. FIG. 1 is a schematic diagram showing the configuration of a fluorescence measurement device 1 according to one embodiment of the present invention. The shape of each component of the illustrated fluorescence measurement apparatus 1 is simplified, and the dimensions of each component are adjusted so as to be easy to see.

A fluorescence measurement device 1 measures the concentration of a measurement target in water to be measured. The fluorescence measurement device 1 includes a housing 10 , a light projecting section 20 , a first light receiving section 30 , a second light receiving section 40 , a supply line 50 , a discharge line 60 and a control device 70 .

The housing 10 includes a main container 11 made of a light-shielding material, and a lid 12 made of a light-shielding material and detachably attached to the main container 11 . As an example, the lid 12 may be formed in a plug shape having an outer thread 121 that screws into an inner thread 111 formed in the opening of the main container 11 .

The housing 10 includes a measurement chamber 13 for storing water to be measured, a light projection path 14 for projecting light into the measurement chamber 13 in a first direction, and a measurement light path for emitting light from the measurement chamber 13 in a direction different from the first direction. 15, a transmitted light path 16 for emitting light from the measurement chamber 13 onto an extension line of the light projection path 14, a supply channel 17 to which the supply line 50 is connected, and a discharge channel 18 to which the discharge line 60 is connected. have.

The projection light path 14 , the measurement light path 15 , and the transmission light path 16 are configured to transmit at least a first measurement light and a second measurement light, which will be described later, without causing water to be measured to flow out of the measurement chamber 13 . As an example, the projection light path 14, the measurement light path 15, and the transmission light path 16 are defined by holes formed in the housing 10 so as to communicate with the measurement chamber 13, and at least the ends on the measurement chamber 13 side are made of a material having translucency. It may be configured to be sealed. The projection light path 14, the measurement light path 15 and the transmission light path 16 are preferably formed by straight holes with a small cross-section so that the direction of incidence and the direction of emission of the light can be defined.

The projected light path 14 and the transmitted light path 16 are coaxially formed on both sides of the measurement chamber 13 . The measurement beam path 15 is formed such that its axis intersects the axes of the projection beam path 14 and the transmission beam path 16 . The axis of the measurement light path 15 is preferably perpendicular to the axes of the projection light path 14 and the transmission light path 16 . Also, the axis of the measurement light path 15 preferably intersects the axes of the projection light path 14 and the transmission light path 16 at the center of the measurement chamber 13 . Further, the projection light path 14, the measurement light path 15 and the transmission light path 16 are arranged so that the length of the light path from the projection light path 14 to the measurement light path 15 and the length of the light path from the projection light path 14 to the transmission light path 16 are equal. is preferred. In FIG. 1, the projection light path 14, the measurement light path 15, and the transmission light path 16 are shown to exist in the same vertical plane in order to show the entire structure. , the axes of the measurement beam path 15 and the transmission beam path 16 are arranged to lie in the same horizontal plane.

The measurement chamber 13 is defined as an internal space of the main container 11 and has an openable and closable lid portion 131 formed by the lid body 12 . By opening the cover portion 131, maintenance such as cleaning of the inside of the measurement chamber 13 can be performed.

The supply channel 17 is preferably formed to open at the bottom of the measurement chamber 13 , and the discharge channel 18 is preferably formed to open at the top of the measurement chamber 13 . Thereby, the water to be measured in the measurement chamber 13 can be efficiently replaced. A discharge channel 18 is formed to allow old measured water to overflow from the upper part of the measurement chamber 13 into a drainage system.

The light projecting unit 20 projects a first measurement light having a first wavelength (for example, ultraviolet light of 365 nm) to the measurement chamber 13 through the light projecting path 14 to excite the measurement target in the water under measurement to generate fluorescence. a light source 21, a second light source 22 that projects a second measurement light having a second wavelength (for example, violet light of 420 nm) that is the same as or similar to the wavelength of the fluorescence to be measured into the measurement chamber 13 through the light projection path 14; 1 light source 21 and second light source 22 are mounted, and at least one of first light source 21 and second light source 22 ( In the illustrated example, it has a light amount adjusting mechanism 24 for adjusting the amount of light projected from the second light source 22 ) to the measurement chamber 13 . The light projecting section 20 preferably has a light projecting section cover 25 that prevents external light from entering the light projecting path 14 .

The first light source 21 and the second light source 22 are typically composed of light emitting diodes. The first light source 21 and the second light source 22, which are small elements such as light emitting diodes, can be arranged adjacently on the same light projecting circuit board 23. FIG. Although the first light source 21 and the second light source 22 are shown enlarged in FIG. 1, the first light source 21 and the second light source 22 are actually small enough to be considered to exist at the same position, and are close to each other. can be placed. By arranging the minute first light source 21 and the second light source 22 adjacent to each other in this manner, the optical axis of the first measurement light and the optical axis of the second measurement light can be substantially aligned. It is possible to reduce the measurement error that may occur due to the difference in the incident directions of the first measurement light and the second measurement light.

The light projecting circuit board 23 has the function of holding the first light source 21 and the second light source 22 in a position where the measurement light can be projected into the measurement chamber 13 through the light projecting path 14 . Further, the light projecting circuit board 23 has a driving circuit that selectively causes the first light source 21 or the second light source 22 to emit light according to a command signal from the control device 70 .

The light amount adjustment mechanism 24 is configured to adjust the intensity of the fluorescence of the measurement target incident on the first light receiving unit 30 when the first measurement light is projected and the first light reception due to the coloring of the water to be measured when the second measurement light is projected. The amount of light from either the first light source 21 or the second light source 22 is limited so that the intensity of scattered light incident on the unit 30 is within the measurable range of the first light receiving unit 30 . In the illustrated example, the light amount adjustment mechanism 24 is arranged to limit the light amount of the second measurement light. Depending on the specifications of the two light sources 22, they may be arranged to limit the light intensity of the first measurement light, or may be arranged to individually limit the light intensity of both the first measurement light and the second measurement light. good. Note that the measurement range of the concentration of the measurement object and the range of the assumed scattering degree can be set arbitrarily.

As the light amount adjusting mechanism 24, a circuit or the like that is incorporated in a driving circuit formed on the light projecting circuit board 23 and adjusts the power supplied to the first light source 21 or the second light source 22 may be used. As a simple arrangement, an aperture can be used to limit the cross-sectional area of the optical path, as shown. By using an aperture as the light amount adjusting mechanism 24, the intensity of the fluorescence received by the first light receiving section 30 and the intensity of the scattered light can be easily made close to each other while the structure is simple.

The first light receiving unit 30 includes a first light receiving element 31 that receives light emitted from the measurement optical path 15 and generates an electric signal corresponding to the intensity of the light, and a first detection circuit board 32 on which the first light receiving element 31 is mounted. and a filter 33 for removing the first wavelength component from the light emitted from the measurement optical path 15 . The first light receiving section 30 preferably has a first light receiving section cover 34 that prevents external light from entering the measurement optical path 15 .

The first light receiving element 31 is typically composed of a photodiode. The first detection circuit board 32 has a detection circuit that amplifies the signal output from the first light receiving element 31 and converts it into a digital signal if necessary, thereby outputting a detection signal that is input to the control device 70 . The filter 33 blocks light in a wavelength band including the first wavelength, while transmitting light in a wavelength band near the second wavelength. Thereby, the first light receiving element 31 detects the intensity of the light of the second wavelength emitted from the measurement optical path 15 . That is, by providing the filter 33, the first light-receiving element 31 does not receive the scattered light of the first measurement light due to coloring when the first measurement light is transmitted through the measurement chamber 13. Since only the intensity of fluorescence can be detected, it is possible to accurately measure the amount of fluorescence light and more accurately calculate the concentration of the object to be measured.

The second light receiving section 40 detects the amounts of the first measurement light and the second measurement light emitted from the measurement chamber 13 through the transmission optical path 16 . That is, the second light receiving section 40 detects the intensity of the first measurement light or the second measurement light that has traveled straight without being absorbed or reflected by the object to be measured. The second light receiving section 40 includes a second light receiving element 41 that receives light emitted from the transmission optical path 16 and generates an electric signal corresponding to the intensity of the received light, and a second detection circuit board 42 on which the second light receiving element 41 is mounted. and have The second light receiving section 40 preferably has a second light receiving section cover 43 that prevents external light from entering the transmission optical path 16 .

Like the first light receiving element 31, the second light receiving element 41 is typically composed of a photodiode. Similarly to the first detection circuit board 32, the second detection circuit board 42 amplifies the signal output from the second light receiving element 41, converts it into a digital signal as necessary, and inputs it to the control device 70. It has a detection circuit that outputs a detection signal. The second light receiving section 40 detects the intensity of the first measurement light or the second measurement light that has traveled straight without being absorbed or reflected by the object to be measured.

The supply line 50 supplies newly measured water to be measured from the supply channel 17 to the measurement chamber 13 . The supply line 50 has an on-off valve 51 that is controlled by the control device 70 and shuts off the supply of the water to be measured.

A discharge line 60 discharges the measured water from the measurement chamber 13 . Specifically, the discharge line 60 extends downward from the discharge channel 18 so as to cause the water to be measured to overflow when new water to be measured is supplied from the supply line 50 .

The control device 70 controls the light projecting section 20 and the supply line 50 and calculates the concentration of the measurement target in the water under measurement from the intensity of the light received by the first light receiving element 31 and the second light receiving element 41 . The control device 70 can be implemented, for example, by causing a computer device having a memory, a CPU, an input/output interface, etc. to execute an appropriate program.

The control device 70 includes a measurement control section 71 , a concentration calculation section 72 , a normal open determination section 73 , and a pause open determination section 74 . The measurement control unit 71, the concentration calculation unit 72, the normal open determination unit 73, and the idle open determination unit 74 are classified functions of the control device 70, and can be clearly distinguished in terms of physical configuration and program configuration. It doesn't have to be something.

The measurement control unit 71 causes the first light source 21 and the second light source 22 of the light projecting unit 20 to sequentially project measurement light, and causes the concentration calculation unit 72 to calculate the detection values of the first light receiving unit 30 and the second light receiving unit 40. Based on this, the concentration of the object to be measured in the water to be measured is calculated. In addition, the measurement control unit 71 opens the on-off valve 51 for a predetermined period of time before the measurement light is projected by the light projecting unit 20 and the concentration of the measurement target is calculated by the concentration calculating unit 72, so that the measurement chamber 13 is Replace the water to be measured inside. Further, the measurement control unit 71 keeps the on-off valve 51 open while at least one of the always open determination unit 73 and the idle open determination unit 74 determines that the cover 131 of the measurement chamber 13 is open. prohibited.

The basic idea is that the light of the second wavelength received by the first light receiving element 31 when the first light source 21 projects the first measurement light is due to the fluorescence of the measurement target in the water to be measured. The concentration of the object to be measured in the water to be measured can be calculated from the received light intensity of the first light receiving element 31 when the first measurement light is projected. However, in the optical path from the projection light path 14 to the measurement optical path 15 in the water to be measured, the first measurement light and the fluorescence of the measurement target are absorbed by the measurement target and coloring, etc., and the received light intensity of the first light receiving element 31 decreases. It may not correspond exactly to the concentration to be measured. For this reason, the concentration calculator 72 calculates the light receiving intensity of the second light receiving element 41 when the first measurement light is projected, the light receiving intensity of the first light receiving element 31 when the second measurement light is projected, and the light receiving intensity of the second light receiving element 41 when the second measurement light is projected. Based on the intensity of received light, an error in the intensity of received light due to absorption and scattering is corrected to calculate an accurate concentration of the object to be measured.

FIG. 2 shows a procedure for measuring the concentration of the measurement target in the fluorescence measurement apparatus 1 controlled by the control device 70. As shown in FIG. Concentration measurement includes a first wavelength measurement step (step S1), a second wavelength measurement step (step S2), a reference concentration calculation step (step S3), a first wavelength absorbance calculation step (step S4), and a second wavelength absorbance calculation step. (Step S5), an absorbance correction step (Step S6), a scattering degree calculation step (Step S7), and a scattering degree correction step (Step S8).

In the first wavelength measurement step of step S1, the first light source 21 projects the first measurement light of the first wavelength, and the received light intensities of the first light receiving element 31 and the second light receiving element 41 are measured.

In the second wavelength measurement process of step S2, the second light source 22 emits the second measurement light of the second wavelength, and the received light intensities of the first light receiving element 31 and the second light receiving element 41 are measured.

In the reference density calculation process of step S3, the reference density, which is the density of the measurement object assuming that there is no absorption of light such as coloring, is calculated based on the received light intensity of the first light receiving element 31 measured in the first wavelength measurement process. calculate. Specifically, the reference concentration can be calculated as a value proportional to the received light intensity of the first light receiving element 31 measured in the first wavelength measurement step. The reference density calculated here is a value that includes an error caused by the influence of coloring or the like.

In the first wavelength absorbance calculation step of step S4, the first absorbance, which is the absorbance of the water to be measured at the first wavelength, is calculated based on the received light intensity of the second light receiving element 41 measured in the first wavelength measurement step. Specifically, the ratio of the light receiving intensity of the second light receiving element 41 measured in the first wavelength measurement step to the light receiving intensity (blank) of the second light receiving element 41 measured with pure water stored in the measurement chamber 13 in advance. , the first absorbance of the water to be measured can be calculated. Note that the first absorbance calculated here does not indicate a strictly accurate degree of light absorption, and may include an error due to light scattering.

In the second wavelength absorbance calculation step of step S5, based on the received light intensity of the second light receiving element 41 measured in the second wavelength measurement step, the absorbance of the water to be measured at the second wavelength is calculated by the same method as in the first wavelength measurement step. A second absorbance is calculated. This second absorbance may also contain errors due to light scattering.

In the absorbance correction step of step S6, the reference concentration calculated in the reference concentration calculation step is corrected by the first absorbance calculated in the first wavelength absorbance calculation step and the second absorbance calculated in the second wavelength absorbance calculation step. The decrease in the received light intensity of the first light-receiving element 31 in the first wavelength measurement step due to light absorption is from the projection light path 14 to the center of the measurement chamber 13 (the intersection of the axis of the light projection path 14 and the axis of the measurement light path 15). Absorption of the first measurement light by the water to be measured on the optical path and absorption of fluorescence by the water to be measured on the optical path from the center of the measurement chamber 13 to the measurement optical path 15 can be considered separately.

Therefore, the absorption of the first measurement light in the optical path from the projection light path 14 to the center of the measurement chamber 13 can be corrected based on the first absorbance, and the light absorption in the optical path from the center of the measurement chamber 13 to the measurement light path 15 Fluorescence absorbance can be corrected based on the second absorbance. For ease of calculation, the optical path length from the projection path 14 to the center of the measuring chamber 13, the optical path length from the center of the measuring chamber 13 to the transmitted optical path 16, and the optical path length from the center of the measuring chamber 13 to the measuring optical path 15 are preferably equal in optical path length.

In the scattering degree calculation step of step S7, the fluorescence excited in the region other than the central portion of the measurement chamber 13 is based on the intensity of light received by the first light receiving element 31 measured in the second wavelength measurement step. A degree of scattering indicating an offset of the received light intensity of the first light receiving element 31 caused by reaching the first light receiving element 31 due to scattering is calculated.

In the scattering degree correcting step of step S8, the value obtained by correcting the reference concentration in the absorbance correcting step is further corrected based on the scattering degree calculated in the scattering degree calculating step. As a result, an accurate concentration value of the measurement object in the water to be measured can be calculated.

The normally open determination section 73 determines that the cover section 131 of the measurement chamber 13 is in the open state when the first light receiving element 31 detects light equal to or greater than a predetermined threshold. That is, the normally open determination unit 73 determines that the maximum amount of light that can be emitted by the first light source 21 and the second light source 22 exceeds the threshold assumed as the maximum amount of light that can be received by the first light receiving element 31. When the first light-receiving element 31 detects light equal to or greater than a preset threshold value, the lid 12 is removed and external light enters the first light-receiving element 31 through the measurement chamber 13 and the measurement optical path 15. judge that it is.

When the light projecting unit 20 is not projecting the measurement light (the first measurement light and the second measurement light), the rest open determination unit 74 detects light from the first light receiving unit 30 or the second light receiving unit 40 . If so, that is, if at least one of the first light-receiving element 31 and the second light-receiving element 41 is receiving light, it is determined that the cover 131 of the measurement chamber 13 is open.

As described above, the measurement chamber 13 storing the water to be measured, the light projection path 14 projecting light into the measurement chamber 13 in the first direction, and the measurement chamber 13 emitting light in a direction different from the first direction. an optical path 15, a transmission optical path 16 for emitting light from the measurement chamber 13 onto an extension of the light projection path 14, and a first measurement light of a first wavelength that excites the object to be measured into the measurement chamber 13 through the light projection path 14 to generate fluorescence. , a second light source 22 that projects a second measurement light having a second wavelength that is the same as or similar to the wavelength of the fluorescence to be measured into the measurement chamber 13 through the light projection path 14, and a measurement light path 15 The fluorescence measurement device 1 includes a first light receiving element 31 that receives light emitted from the transmission light path 16 and a second light receiving element 41 that receives light emitted from the transmission optical path 16. In consideration of this, the concentration of the object to be measured can be measured relatively accurately.

In addition, since the fluorescence measurement apparatus 1 includes a light amount adjustment mechanism 24 that adjusts the amount of light projected from at least one of the first light source 21 and the second light source 22 to the measurement chamber 13, the measurement target when the first measurement light is projected. , and the intensity of scattered light due to coloring or the like during projection of the second measurement light can be kept within the measurable range of the first light receiving element 31 . Therefore, the fluorescence measurement apparatus 1 can measure the intensity of fluorescence and the intensity of scattered light using the same first light receiving element 31, so the number of light receiving elements is small and the configuration is simple. In addition, since the fluorescence measurement device 1 measures the intensity of fluorescence and the intensity of scattered light in the same optical path, measurement errors due to partial dirt or the like on the device are less likely to occur.

In addition, since the fluorescence measurement apparatus 1 includes the always open determination unit 73 and the idle open determination unit 74 that detect opening of the lid 131 of the measurement chamber 13, the fluorescence measurement apparatus 1 keeps the lid 131 open. In this state, the water to be measured is newly supplied to the measurement chamber 13 from the supply line 50, and the trouble of the water to be measured spouting out from the open lid portion 131 can be prevented.

In addition, the fluorescence measurement apparatus 1 opens the on-off valve 51 for a predetermined period of time before emitting the measurement light, and measures the concentration by emitting the measurement light with the on-off valve 51 closed. is open for a short time, and the risk of opening the cover 131 by mistake while the on-off valve 51 is open can be reduced.

In addition, the fluorescence measurement apparatus 1 is provided with amplification circuits for amplifying the detection signals of the light receiving sections 30 and 40 on the first detection circuit board 32 and the second detection circuit board 42, thereby setting the intensity of the measurement light to be small. Since the detection sensitivity of external light can be substantially improved, the opening of the lid portion 131 can be detected more reliably.

Although preferred embodiments of the fluorescence measurement apparatus of the present invention have been described above, the present invention is not limited to the above-described embodiments, and can be modified as appropriate.

As an example, a calculation formula may be used in which the concentration of the measurement target is directly calculated by substituting the detected value of the received light intensity without calculating at least one of the reference concentration, the first absorbance, the second absorbance and the scattering degree. .

In addition, when it is difficult to provide a filter that blocks only the light of the first wavelength without blocking the light of the second wavelength, the filter is not provided in the first measurement unit, and the value calculated from the measurement value of the second measurement light is used. The correction may be performed assuming that the degree of scattering at the second wavelength and the degree of scattering at the first wavelength are approximately the same.

1 fluorescence measurement device 10 housing 11 main container 111 inner screw 12 lid 121 outer screw 13 measurement chamber 131 lid 14 light projection path 15 measurement light path 16 transmission light path 17 supply channel 18 discharge channel 20 light projection part 21 first light source 22 Second light source 23 Light emitting circuit board 24 Light amount adjusting mechanism 25 Translucent part cover 30 First light receiving part 31 First light receiving element 32 First detection circuit board 33 Filter 34 First light receiving part cover 40 Second light receiving part 41 Second light receiving Element 42 Second detection circuit board 43 Second light receiving unit cover 50 Supply line 51 On-off valve 60 Discharge line 70 Control device 71 Measurement control unit 72 Concentration calculation unit 73 Always open determination unit 74 Open determination unit at rest

Claims (6)

A fluorescence measuring device for measuring the concentration of a measurement target in water to be measured,
a measurement chamber for storing the water to be measured;
a light projection path for projecting light into the measurement chamber in a first direction;
a measurement optical path that emits light from the measurement chamber in a direction different from the first direction;
a transmitted light path for emitting light from the measurement chamber onto an extension line of the light projection path;
a first light source that projects a first measurement light having a first wavelength that excites the object to be measured and causes fluorescence to be emitted into the measurement chamber through the light projection path;
a second light source that projects a second measurement light having a second wavelength that is the same as or similar to the wavelength of the fluorescence of the measurement target into the measurement chamber through the light projection path;
a first light receiving element that receives light emitted from the measurement optical path;
a second light receiving element that receives light emitted from the transmission optical path;
a light amount adjusting mechanism that adjusts the amount of light projected from at least one of the first light source and the second light source to the measurement chamber;
A fluorometer. 2. The fluorescence measuring apparatus according to claim 1, wherein said light quantity adjusting mechanism has a diaphragm that restricts an optical path of at least one of said first light source and said second light source. 3. The fluorescence measurement device according to claim 1, wherein said first light source and said second light source are arranged adjacently on the same substrate. 3. The fluorescence measurement apparatus according to claim 1, further comprising a filter that removes the first wavelength component from the light emitted from the measurement optical path. 5. The apparatus according to any one of claims 1 to 4, further comprising a constantly open determination unit that determines that the measurement chamber is open when the first light receiving element detects light equal to or greater than a predetermined threshold. Fluorometer. If the first light-receiving element or the second light-receiving element detects light when the first measurement light and the second measurement light are not projected, it is determined that the measurement chamber is open. 6. The fluorescence measurement device according to claim 1, further comprising a rest-time open determination unit.

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