JP2022524052A - 抗体の選択方法 - Google Patents

抗体の選択方法 Download PDF

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Publication number
JP2022524052A
JP2022524052A JP2021552898A JP2021552898A JP2022524052A JP 2022524052 A JP2022524052 A JP 2022524052A JP 2021552898 A JP2021552898 A JP 2021552898A JP 2021552898 A JP2021552898 A JP 2021552898A JP 2022524052 A JP2022524052 A JP 2022524052A
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cells
cell
binding partner
target polypeptide
cell population
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JP2021552898A
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JP2022524052A5 (https=
Inventor
ナンシー ロペス‐アントン
ネイサン ロバートソン
ティモシー ジョーンズ
ライアン ケイウッド
トーマス ペイン
リチャード パーカー‐マヌエル
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Oxford Genetics Ltd
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Oxford Genetics Ltd
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Priority claimed from GBGB1903233.3A external-priority patent/GB201903233D0/en
Priority claimed from GBGB1903270.5A external-priority patent/GB201903270D0/en
Priority claimed from GB201913333A external-priority patent/GB201913333D0/en
Priority claimed from GB201914819A external-priority patent/GB201914819D0/en
Application filed by Oxford Genetics Ltd filed Critical Oxford Genetics Ltd
Publication of JP2022524052A publication Critical patent/JP2022524052A/ja
Publication of JP2022524052A5 publication Critical patent/JP2022524052A5/ja
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • G—PHYSICS
    • G01—MEASURING; TESTING
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/7051—T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2818—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against CD28 or CD152
    • C—CHEMISTRY; METALLURGY
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    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
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    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/286—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against neuromediator receptors, e.g. serotonin receptor, dopamine receptor
    • C—CHEMISTRY; METALLURGY
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    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
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    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
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    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/33—Fusion polypeptide fusions for targeting to specific cell types, e.g. tissue specific targeting, targeting of a bacterial subspecies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/42—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a HA(hemagglutinin)-tag
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/10041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/10043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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JP2021552898A 2019-03-08 2020-03-06 抗体の選択方法 Pending JP2022524052A (ja)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
GBGB1903233.3A GB201903233D0 (en) 2019-03-08 2019-03-08 Method of selecting for antibodies
GB1903233.3 2019-03-08
GB1903270.5 2019-03-11
GBGB1903270.5A GB201903270D0 (en) 2019-03-11 2019-03-11 Method of selecting for antibodies
GB201913333A GB201913333D0 (en) 2019-09-16 2019-09-16 Method of selecting for antibodies
GB1913333.9 2019-09-16
GB201914819A GB201914819D0 (en) 2019-10-14 2019-10-14 Method of selecting for antibodies
GB1914819.6 2019-10-14
PCT/GB2020/050538 WO2020183133A1 (en) 2019-03-08 2020-03-06 Method of selecting for antibodies

Publications (2)

Publication Number Publication Date
JP2022524052A true JP2022524052A (ja) 2022-04-27
JP2022524052A5 JP2022524052A5 (https=) 2023-03-14

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JP2021552898A Pending JP2022524052A (ja) 2019-03-08 2020-03-06 抗体の選択方法

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US (1) US20220154174A1 (https=)
EP (1) EP3935389A1 (https=)
JP (1) JP2022524052A (https=)
KR (1) KR20210137128A (https=)
CN (1) CN113544509A (https=)
AU (1) AU2020235455A1 (https=)
SG (1) SG11202108320PA (https=)
WO (1) WO2020183133A1 (https=)

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CN110892064A (zh) 2017-07-25 2020-03-17 牛津遗传学有限公司 腺病毒载体
GB201901571D0 (en) 2019-02-05 2019-03-27 Oxford Genetics Ltd Inducible AAV sysyem
TW202323529A (zh) 2021-10-18 2023-06-16 美商再生元醫藥公司 包括腺病毒相關病毒聚核苷酸的真核細胞

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JP2017108737A (ja) * 2007-08-22 2017-06-22 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフ 活性化可能な結合ポリペプチドおよびその同定方法ならびに使用
WO2018114772A1 (en) * 2016-12-21 2018-06-28 F. Hoffmann-La Roche Ag Assay for determining antibody or ligand binding and function
WO2018167481A1 (en) * 2017-03-15 2018-09-20 Oxford Genetics Limited Method of selecting for antibodies

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JP2015529826A (ja) * 2012-08-31 2015-10-08 ザ スクリプス リサーチ インスティテュート 真核生物細胞の調節因子に関する方法及び組成物
WO2018114772A1 (en) * 2016-12-21 2018-06-28 F. Hoffmann-La Roche Ag Assay for determining antibody or ligand binding and function
WO2018167481A1 (en) * 2017-03-15 2018-09-20 Oxford Genetics Limited Method of selecting for antibodies

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AU2020235455A1 (en) 2021-10-28
EP3935389A1 (en) 2022-01-12
US20220154174A1 (en) 2022-05-19
KR20210137128A (ko) 2021-11-17
WO2020183133A1 (en) 2020-09-17
CN113544509A (zh) 2021-10-22
SG11202108320PA (en) 2021-09-29

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