JP2022149691A - Liposome composition for cosmetics - Google Patents

Abstract

To provide a liposome composition for cosmetics that comprises a liposome having an increased content of a beauty component.SOLUTION: A liposome composition for cosmetics comprises: liposomes that comprise polyoxyethylene phytosterol, a lecithin, and at least one of cholesterol and a phytosterol other than polyoxyethylene phytosterol with an average particle size of 100 nm-350 nm; an internal aqueous phase that comprises water, ethanol and a beauty component and is contained in each liposome; and an external aqueous phase that comprises water and ethanol and disperses the liposomes.SELECTED DRAWING: Figure 1

Description

The present disclosure relates to cosmetic liposomal compositions.

A liposome is a closed endoplasmic reticulum formed by a lipid bilayer membrane containing a lipid such as lecithin, and has an aqueous phase (inner aqueous phase) within the space of the closed vesicle. In addition, liposomes usually exist in a dispersed state in an aqueous solution (external aqueous phase) outside closed vesicles.

In recent years, application of liposomes containing pharmaceutical ingredients or cosmetic ingredients in the internal water phase to pharmaceutical compositions, cosmetics, and the like has been studied.

For example, Patent Document 1 proposes a liposome composition containing liposomes having an average particle size of 5 nm to 100 nm or less in an internal aqueous phase containing a pharmaceutical ingredient.
Further, Patent Document 2 proposes a cosmetic liposome composition containing liposomes having an average particle size of 82 nm or less and containing an oil component in the internal water phase.
Furthermore, Patent Document 3 proposes a liposome composition for cosmetics containing liposomes having an average particle size of 50 nm or less and containing cosmetic ingredients in the internal water phase.
Moreover, Patent Document 4 proposes a cosmetic composition containing a disc-shaped bicelle structure formed by a lipid bilayer membrane instead of a liposome.
In addition, Patent Document 5 proposes a cosmetic containing vesicles contained in an ethanol-containing external aqueous phase.

Japanese Patent No. 6251385 Japanese Patent Application Laid-Open No. 2020-93992 Japanese Patent No. 5064717 WO2017/090740 JP 2016-56198 A

When applying liposomes encapsulating cosmetic ingredients to cosmetics, there is a demand for liposomes capable of encapsulating a larger amount of cosmetic ingredients.
Now, the present inventors have found that the liposomes contained in the compositions proposed in Patent Documents 1 to 3, the bicelle structure contained in the composition proposed in Patent Document 4, and the composition proposed in Patent Document 5. We obtained new knowledge that there is room for improvement in the encapsulation rate of beauty ingredients in vesicles that contain beauty ingredients.

In addition, from the viewpoint of encapsulation rate of cosmetic ingredients, it is desirable that the liposomes contained in the liposome composition have a large proportion of liposomes having a single-lamellar structure (unilamellar vesicles) with a single lipid bilayer.
The present inventors have further found that the compositions in Patent Documents 2, 3, and 5 have a small ratio of liposomes having a single lamellar structure, and the encapsulation rate of cosmetic ingredients is not sufficient.

The present disclosure has been made in view of the above circumstances, and the problem to be solved is to provide a liposome composition for cosmetics containing liposomes with an improved encapsulation rate of cosmetic ingredients.

<1> Liposomes containing polyoxyethylene phytosterol, lecithin, and at least one of cholesterol and phytosterols other than polyoxyethylene phytosterol, and having an average particle size of 100 nm to 350 nm;
A liposome composition for cosmetics, comprising an internal aqueous phase containing water, ethanol, and a cosmetic component and encapsulated in the liposome, and an external aqueous phase containing water and ethanol, in which the liposome is dispersed.
<2> The liposome composition for cosmetics according to <1> above, wherein the polyoxyethylene phytosterol has an average addition mole number of oxyethylene groups of 10 or more.
<3> The liposome composition for cosmetics according to <1> or <2>, wherein the content of the polyoxyethylene phytosterol in the liposome composition for cosmetics is 0.1% by mass or more.
<4> The cosmetic liposome composition according to any one of <1> to <3>, wherein the content of ethanol relative to the total mass of the cosmetic liposome composition is 0.2% by mass to 30% by mass. Liposomal composition.
<5> The content of at least one of the cholesterol and the phytosterol other than the polyoxyethylene phytosterol is 0.1% by mass to 0.5% by mass relative to the total mass of the liposome composition for cosmetics, <1> The cosmetic liposome composition according to any one of <4>.
<6> The liposome composition for cosmetics according to any one of <1> to <5>, wherein the cosmetic ingredient is a water-soluble cosmetic ingredient.
<7> The liposome composition for cosmetics according to any one of <1> to <6>, wherein the cosmetic ingredient is at least one selected from caffeine, pyridoxine hydrochloride and nicotinamide.
<8> The cosmetic according to any one of <1> to <7>, wherein the ratio of the total number of liposomes having a single lamella structure to the total number of liposomes contained in the cosmetic liposome composition is 70% or more. Liposomal composition for use.
<9> The cosmetic liposome composition according to any one of <1> to <8>, which is a cosmetic.

According to the present disclosure, it is possible to provide a liposome composition for cosmetics containing liposomes with an improved encapsulation rate of cosmetic ingredients.

1 shows a TEM (Transmission Electron Microscope) image of the cosmetic liposome composition obtained in Example 1-2. 1 shows a TEM image of the cosmetic liposome composition obtained in Example 3-1. 1 shows a TEM image of the cosmetic liposome composition obtained in Example 3-2. 1 shows a TEM image of the cosmetic liposome composition obtained in Comparative Example 1-2. 1 shows TEM images of cosmetic liposome compositions obtained in Comparative Examples 1-5.

Hereinafter, embodiments for implementing the present disclosure will be described in detail. However, the present invention is not limited to the following embodiments. In the embodiments described below, the constituent elements are not essential unless otherwise specified. The same applies to numerical values and their ranges, which do not limit the present disclosure.

In the present disclosure, the numerical range indicated using "-" includes the numerical values before and after "-" as the minimum and maximum values, respectively.
In the numerical ranges described step by step in the present disclosure, the upper limit or lower limit of one numerical range may be replaced with the upper or lower limit of another numerical range described step by step. . Moreover, in the numerical ranges described in the present disclosure, the upper or lower limits of the numerical ranges may be replaced with the values shown in the examples.
In the present disclosure, each component may contain multiple types of applicable compounds. When multiple types of substances corresponding to each component are present in the composition, the content of each component means the total content of the multiple types of substances present in the composition unless otherwise specified.

In the present disclosure, a "liposome" is a closed endoplasmic reticulum formed by a lipid bilayer membrane.
A structure with a single lipid bilayer is called a single lamellar structure, and a structure with multiple lipid bilayers is called a multilamellar structure.
In the present disclosure, "inner aqueous phase" means the solution present in the closed endoplasmic reticulum.
In the present disclosure, "external aqueous phase" means a solution in which liposomes are dispersed.

In the present disclosure, "cholesterol" is a generic term for sterols contained in animals, and "phytosterol" is a generic term for sterols contained in plants.

In the present disclosure, the term “beauty ingredient” means an ingredient that acts on the body such as the skin and is an active ingredient used in cosmetics and the like.
Moreover, "water-soluble" means that the solubility in 100 g of water at a liquid temperature of 22°C and pH of 7.0 is 0.1 g or more.

In the present disclosure, "average particle size" means volume average particle size determined by dynamic light scattering method.
Dynamic light scattering particle size analyzers include concentrated particle size analyzer FPAR-1000 (manufactured by Otsuka Electronics Co., Ltd.), Nanotrac UPA (manufactured by Nikkiso Co., Ltd.), and dynamic light scattering particle size distribution analyzer LB-. 550 (manufactured by HORIBA, Ltd.) and the like.
The average particle size is measured by diluting the liposome composition for cosmetics to 10-fold mass with pure water and carrying out the measurement at room temperature (for example, 25°C).
As for "pure water", pure water obtained by an ultrapure water production apparatus manufactured by Merck Co., Ltd. is used.

In the present disclosure, "the ratio of the total number of liposomes having a single-lamellar structure to the total number of liposomes contained in the liposome composition for cosmetics" means that the liposomes contained in the liposome composition for cosmetics are in an ice-embedded state, and the permeation type It is determined by observing with an electron microscope (TEM) and counting the total number of liposomes contained in the cosmetic liposome composition and the total number of liposomes having a single lamellar structure.
More specifically, first, a transmission electron microscope is used to obtain a TEM image of the cosmetic liposome composition at a magnification of 50000 times.
By counting the total number of liposomes present within a circle with a radius of 2 μm and the total number of liposomes having a single lamellar structure at each of the three arbitrary points in the TEM image, the "total number of liposomes contained in the liposome composition for cosmetics The ratio of the total number of liposomes having a single lamellar structure” is determined.
In the present disclosure, "ratio of the total number of liposomes having a single lamella structure to the total number of liposomes contained in the cosmetic liposome composition" is the average value of three locations.
In the present disclosure, the encapsulation rate of cosmetic ingredients in liposomes can be evaluated by the "average particle size of liposomes" and the ratio of the total number of liposomes having a single lamella structure to the total number of liposomes contained in the liposome composition for cosmetics. can.

(Liposome composition for cosmetics)
The cosmetic liposome composition of the present disclosure contains polyoxyethylene phytosterol, lecithin, and at least one of cholesterol and phytosterols other than polyoxyethylene phytosterol, and has an average particle size of 100 nm to 350 nm, water, ethanol and It includes an internal aqueous phase that contains cosmetic ingredients and is encapsulated in the liposomes, and an external aqueous phase that contains water and ethanol and disperses the liposomes.

According to the liposome composition for cosmetics of the present disclosure, it is possible to provide a liposome composition for cosmetics containing liposomes with an excellent encapsulation rate of cosmetic ingredients.

The reason why the above effects are exhibited is presumed as follows, but is not limited thereto.
In the liposome composition for cosmetics of the present disclosure, the liposomes contain polyoxyethylene phytosterol to inhibit the approach of the lipid bilayer membranes, and the polyoxyethylene phytosterol contributes to the formation of the liposomes. It is presumed that the average particle size of liposomes and the ratio of liposomes having a single lamellar structure are increased, and the encapsulation rate of cosmetic ingredients is improved.

From the viewpoint of improving the encapsulation rate of cosmetic ingredients, the ratio of the polyoxyethylene phytosterol content to the ethanol content (polyoxyethylene phytosterol content/ethanol content) in the liposome composition for cosmetics is based on mass. is preferably from 0.001 to 1, more preferably from 0.002 to 0.98, even more preferably from 0.03 to 0.97.
In addition, by setting the ratio of the polyoxyethylene phytosterol content to the ethanol content within the above numerical range, the liposomes aggregate over time, the particle size of the liposomes increases, and the liposome composition for cosmetics is suspended. It is possible to suppress the occurrence of turbidity.
Furthermore, by setting the ratio of the content of polyoxyethylene phytosterol to the content of ethanol within the above numerical range, aggregated liposomes coalesce and become multilamellar, resulting in a decrease in the encapsulation rate of cosmetic ingredients. can be suppressed.
The ability to suppress aggregation of liposomes described above is hereinafter also referred to as stability over time.
The ratio of the content of polyoxyethylene phytosterol to the content of ethanol in the cosmetic liposome composition is obtained by rounding off to the fourth decimal place.

In the cosmetic liposome composition, the ratio of the polyoxyethylene phytosterol content to the cholesterol content (polyoxyethylene phytosterol content/cholesterol content) is preferably 2 or less on a mass basis, It is more preferably 1.7 or less, and even more preferably 1 or less.
When the ratio of the content of polyoxyethylene phytosterol to the content of cholesterol in the liposome composition for cosmetics is 2 or less on a mass basis, the proportion of liposomes having a single lamellar structure in the liposome composition for cosmetics is reduced. It increases more, and the encapsulation rate of the cosmetic ingredient is further improved.
The lower limit of the ratio of the polyoxyethylene phytosterol content to the cholesterol content in the cosmetic liposome composition is not particularly limited, but from the viewpoint of liposome formation, it is 0.1 or more. is preferably
The ratio of the content of polyoxyethylene phytosterol to the content of cholesterol in the cosmetic liposome composition is obtained by rounding off to the second decimal place.

(Liposome)
In the cosmetic liposome composition of the present disclosure, the liposomes have an average particle size of 100 nm to 350 nm.
When the average particle size of the liposome is 100 nm or more, the encapsulation rate of the cosmetic ingredient is improved. Moreover, when the average particle size of the liposomes is 100 nm or more, the proportion of liposomes having a single lamella structure in the cosmetic liposome composition tends to increase.
Liposomes with an average particle size of more than 350 have low stability over time, and tend to become multilamellar due to aggregation and coalescence of liposomes over time, resulting in a decrease in encapsulation rate of cosmetic ingredients. When the liposome has an average particle size of 350 nm or less, the multilamellarization can be suppressed. Moreover, when the average particle size of the liposomes is more than 350 nm, the produced liposome composition for cosmetics tends to have an increased turbidity. By setting the average particle size of the liposomes to 350 nm or less, it is possible to suppress an increase in turbidity of the liposome composition for cosmetics, and the appearance of the liposome composition for cosmetics tends to be excellent. Further, when the average particle size of the liposomes is 350 nm or less, the penetration of the liposome composition for cosmetics into the skin or the like tends to be improved.
The average particle size of the liposomes is preferably 110 nm to 330 nm, more preferably 120 nm to 320 nm, and even more preferably 150 nm to 300 nm, from the viewpoints of encapsulation rate of cosmetic ingredients and stability over time.

The liposomes are preferably liposomes having a single-lamellar structure (also called unilamellar vesicles) from the viewpoint of improving the encapsulation rate of cosmetic ingredients. The percentage of the total number is preferably 50% or more, more preferably 70% or more.
The liposome composition may contain liposomes having a multilamellar structure (also called multilamellar vesicles).

The liposomes contain polyoxyethylene phytosterol, lecithin, and at least one of cholesterol and phytosterols other than polyoxyethylene phytosterol.
More specifically, the lipid bilayer membrane that constitutes the liposome contains the above components. Each component is explained below.

(polyoxyethylene phytosterol)
Polyoxyethylene phytosterols are phytosterols having an oxyethylene group represented by (CH ₂ CH ₂ —O) _n .
In the present disclosure, phytosterols include hydrogenated forms of phytosterols (phytostanols).
Specific examples of phytosterols include sitosterol, stigmasterol, fucosterol, spinasterol, brassicasterol and hydrogenated products thereof.

From the viewpoint of improving the encapsulation rate of cosmetic ingredients, the average added mole number of oxyethylene groups in the polyoxyethylene phytosterol is preferably 5 or more, preferably 10 or more, and more preferably 20 or more.
In addition, by setting the average added mole number of oxyethylene groups of polyoxyethylene phytosterol to 5 or more, the ratio of liposomes having a single lamellar structure in the liposome composition for cosmetics can be increased, and the cosmetic ingredients of liposomes can be increased. The encapsulation rate can be further improved. For the average number of added moles of oxyethylene groups in polyoxyethylene phytosterol, catalog values can be referred to.

Examples of commercially available polyoxyethylene phytosterols include Nikkol (registered trademark) BPS-5 (average addition mole number of oxyethylene group: 5) manufactured by Nikko Chemicals Co., Ltd., Nikkol (registered trademark) BPS-10 (average number of oxyethylene groups: addition mole number 10), Nikkol (registered trademark) BPS-20 (average addition mole number of oxyethylene group 20) and Nikkol (registered trademark) BPS-30 (average addition mole number of oxyethylene group 30), Nikkol (registered trademark ) BPSH-25 (average number of moles of oxyethylene group added: 25, ethylene oxide adduct of hydrogenated phytosterol), manufactured by Nihon Emulsion Co., Ltd. EMALEX (registered trademark) PS-5 (average number of moles of oxyethylene group added: 5) , EAMLEX (registered trademark) PS-10 (average number of added moles of oxyethylene group 10), Emalex (registered trademark) PS-20 (average number of added moles of oxyethylene group 20) and Emalex (registered trademark) PS-30 ( Examples include oxyethylene group average added mole number of 30), Emalex (registered trademark) PS-25 (oxyethylene group average added mole number of 25), and the like.

The content of polyoxyethylene phytosterol relative to the total mass of the liposome composition for cosmetics is preferably 0.05% by mass or more, more preferably 0.1% by mass or more, and 0.23% by mass or more. is more preferable.
When the content of polyoxyethylene phytosterol is 0.05% by mass or more, the ratio of liposomes having a single lamellar structure in the liposome composition for cosmetics can be increased, and the encapsulation rate of cosmetic ingredients in liposomes can be increased. can be improved.
In addition, the content of polyoxyethylene phytosterol with respect to the total mass of the cosmetic liposome composition is preferably 1% by mass or less, more preferably 0.70% by mass or less. When the content of polyoxyethylene phytosterol is 1% by mass or less, formation of disk particles or micelles instead of liposomes can be suppressed.

(lecithin)
Examples of lecithin include natural lecithin obtained from plants such as soybean, rapeseed, sunflower, safflower, peanut, cotton seed, corn, rice, and barley, egg yolk, and derivatives thereof (hereinafter also referred to as lecithin derivative). be done.
Examples of lecithin derivatives include hydrogenated lecithin, compounds obtained by introducing polyethylene glycol, aminoglycans, etc. into lecithin phospholipids, and the like.
Among the above, hydrogenated lecithin is particularly preferable from the viewpoint of oxidation stability.

From the viewpoint of stability over time of the cosmetic liposome composition, the content of phosphatidylcholine (hereinafter also referred to as PC content) relative to the total mass of lecithin is preferably 80% by mass or more, and is 85% by mass or more. is more preferable, and 90% by mass or more is even more preferable.
The PC content is measured by TLC-FID method or HPLC method for the phospholipid composition.

Commercially available lecithins include COATSOME NC-21 manufactured by NOF Corporation (PC content of 90% by mass or more), Nikkol (registered trademark) Lecinol S-10E manufactured by Nikko Chemicals Co., Ltd. (PC content of 75% by mass to 85% by mass or more).

From the viewpoint of lipid bilayer formation and stability over time, the content of lecithin with respect to the total mass of the cosmetic liposome composition is preferably 0.3% by mass to 10% by mass, and 0.4% by mass. % to 5% by mass, more preferably 0.5% to 3% by mass.

(Phytosterols other than cholesterol and polyoxyethylene phytosterols)
In this disclosure, cholesterol includes cholestanol and cholesterol derivatives.
Cholesterol includes cholesterol, dihydrocholesterol, dehydrocholesterol, cholesteryl oleate, cholesteryl isostearate, cholesteryl hydroxystearate, polyoxyethylene cholesteryl ether and the like.
Moreover, phytosterols other than polyoxyethylene phytosterol refer to phytosterols having no polyoxyethylene group.
Moreover, from the viewpoint of encapsulation rate of cosmetic ingredients, the cosmetic liposome composition of the present disclosure preferably contains at least cholesterol.

The content of at least one of cholesterol and phytosterols other than polyoxyethylene phytosterol is preferably 0.05% by mass to 0.7% by mass, more preferably 0.1% by mass, relative to the total mass of the liposome composition for cosmetics. It is more preferably 0.5% by mass, and even more preferably 0.2% by mass to 0.4% by mass.
By setting the content of at least one of cholesterol and phytosterols other than polyoxyethylene phytosterol within the above numerical range, the proportion of liposomes having a single lamellar structure in the liposome composition for cosmetics can be increased, and the liposome cosmetic ingredient can be obtained. can further improve the encapsulation rate of
In addition, in the present disclosure, "the content of at least one of cholesterol and phytosterols other than polyoxyethylene phytosterol relative to the total mass of the liposome composition for cosmetics" means that when the liposome composition for cosmetics contains only cholesterol, means the content of cholesterol, when the liposome composition for cosmetics contains only phytosterols, it means the content of cholesterol, and when the liposome composition for cosmetics contains cholesterol and phytosterols, It means the sum of these contents.

(Inner water phase and outer water phase)
The cosmetic liposomal composition of the present disclosure comprises an internal aqueous phase and an external aqueous phase.
The internal aqueous phase contains at least water, ethanol and cosmetic ingredients, and the external aqueous phase contains at least water and ethanol. By containing ethanol in the inner aqueous phase and the outer aqueous phase, liposome formation and stability over time can be improved.

(water)
Examples of water include ion-exchanged water, pure water, purified water, and tap water. Among these, purified water is preferred from the viewpoint of applicability to cosmetics.

The water content relative to the total mass of the liposome composition for cosmetics is not particularly limited, and is preferably 1% by mass to 99% by mass, more preferably 10% by mass to 95% by mass, and 20% by mass. % to 90 mass %.
In the present disclosure, the "content of water relative to the total mass of the liposome composition for cosmetics" refers to the content of water contained in the internal aqueous phase and the external aqueous phase relative to the total mass of the liposome composition for cosmetics. means the sum of the water contents contained in

(ethanol)
From the viewpoint of stability over time, the content of ethanol relative to the total mass of the cosmetic liposome composition is preferably 0.2% by mass to 30% by mass.
Moreover, the content of ethanol relative to the total mass of the liposome composition for cosmetics may be 3% by mass or more, 9% by mass or more, or 15% by mass or more.
In addition, the content of ethanol relative to the total mass of the liposome composition for cosmetics may be 27% by mass or less, 25% by mass or less, or 23% by mass or less, or 10% by mass. % or less.
In the present disclosure, the "content of ethanol relative to the total mass of the liposome composition for cosmetics" means the content of ethanol contained in the internal aqueous phase and the external aqueous phase relative to the total mass of the liposome composition for cosmetics. means the sum of the ethanol content in the
In addition, anhydrous ethanol is included in ethanol.

(beauty ingredient)
Cosmetic ingredients include ascorbic acid, ascorbyl stearate, sodium ascorbate, disodium ascorbate sulfate, aspartic acid, aspartame, acetylglucosamine, acetylglutamic acid, acetylcysteine, acetylpantothenyl ethyl ester, disodium adenosine triphosphate. , adenosine monophosphate disodium, ε-aminocaproic acid, γ-aminobutyric acid, allantoin, allantoin-β-glycyrrhetinic acid, albumin, inosit, erythritol, glucosamine hydrochloride, pyridoxine hydrochloride, oxyproline, orotic acid, hydrolyzed elastin, caffe Yin hydrate, sodium chondroitin sulfate, cyanocobalamin, water-soluble elastin, taurine, sodium palmitoyl methyl taurate, sodium myristoyl methyl taurate, thiamine hydrochloride, theanine, deoxyribonucleic acid, ascorbyl palmitate, pantothenyl alcohol, sodium pantothenate, pantothenic acid Calcium, pyridoxine, phytic acid, placenta extract, flavin adenine dinucleotide disodium dihydrate, anhydrous caffeine, riboflavin, riboflavin butyrate, riboflavin phosphate, resorcinol, levulinic acid, glycine, arginine, lysine solution, lauroyl lysine, Aspartic acid, palmitoyl aspartic acid, cysteine, methionine, glutamic acid, threonine, serine, tyrosine, histidine, proline, glutathione, salicylic acid, nicotinamide, retinyl palmitate, alginic acid, γ-undecalactone, perilla oil, estradiol, estrone, Ergocalciferol, β-carotene, glucosamine, cholecalciferol, tocopherol acetate, retinol acetate, salicylic acid, shikonin, ascorbyl dipalmitate, pyridoxine dipalmitate, natural vitamin E, α-tocopherol, horse oil, γ-nonalactone, bisabolol , vitamin A oil, hinokitiol, fumaric acid, powdered vitamin A, tocopherol linoleate, δ-tocopherol, tocopherol linoleate, isoleucine, phenylalanine, valine, leucine, ascorbyl tetra-2-hexyldecanoate, and the like. Caffeine includes anhydrous caffeine.
From the viewpoint of solubility, the cosmetic ingredient contained in the inner water phase is preferably a water-soluble cosmetic ingredient. Acid, Aspartame, Acetylglucosamine, Acetylglutamic Acid, Acetylcysteine, Acetylpantothenyl Ethyl Ester, Disodium Adenosine Triphosphate, Disodium Adenosine Monophosphate, ε-Aminocaproic Acid, γ-Aminobutyric Acid, Allantoin, Allantoin-β-Glycyrrhetinic Acid, Albumin , inosit, erythritol, glucosamine hydrochloride, pyridoxine hydrochloride, oxyproline, orotic acid, hydrolyzed elastin, caffeine hydrate, sodium chondroitin sulfate, cyanocobalamin, water-soluble elastin, taurine, sodium palmitoyl methyl taurate, sodium myristoyl methyl taurate, thiamine Hydrochloride, theanine, deoxyribonucleic acid, ascorbyl palmitate, pantothenyl alcohol, sodium pantothenate, calcium pantothenate, pyridoxine, phytic acid, placenta extract, flavin adenine dinucleotide disodium dihydrate, anhydrous caffeine, riboflavin, riboflavin butyric acid Ester, riboflavin phosphate, resorcinol, levulinic acid, glycine, arginine, lysine solution, lauroyllysine, aspartic acid, palmitoyl aspartic acid, cysteine, methionine, glutamic acid, threonine, serine, tyrosine, histidine, proline, glutathione, salicylic acid, nicotine Acid amides and the like are preferred.
Furthermore, among the above, from the viewpoint of anti-aging, anti-wrinkle, anti-blemish, anti-tension, etc., the inner water phase more preferably contains at least one selected from caffeine, pyridoxine hydrochloride and nicotinic acid amide.
Note that the cosmetic component may also be contained in the external aqueous phase.

The content of the cosmetic component with respect to the total mass of the liposome composition for cosmetics is preferably 0.3% by mass to 5% by mass, more preferably 0.5% by mass to 3% by mass.

The internal aqueous phase and external aqueous phase may contain other components such as physiological saline, sugar, pH adjusters, preservatives and the like. Sugars include glucose, fructose, lactose, sucrose, trehalose, lactulose, maltitol and the like. Examples of pH adjusters include sodium hydrogen phosphate, sodium dihydrogen phosphate, disodium hydrogen phosphate anhydrous, sodium hydroxide (caustic soda), citric acid, acetic acid, triethanolamine and the like. Antiseptics include methyl paraoxybenzoate and the like.

The pH of the inner water phase and the outer water phase is not particularly limited, but is preferably 5-9, more preferably 7-8. The pH of the inner water phase and the outer water phase can be adjusted by using the pH adjusters described above.

The method for producing the cosmetic liposome composition of the present disclosure will be described below, but is not limited thereto.
A liposome composition for cosmetics can be produced by mixing an oil phase composition and an aqueous phase composition, followed by stirring and emulsification to form liposomes.

By mixing, stirring, and emulsifying the oil phase composition and the aqueous phase composition, the oil phase composition and the aqueous phase composition are emulsified into an O/W type (oil-in-water type), and the liposome, the internal aqueous phase, and the A cosmetic liposomal composition comprising an external aqueous phase is produced.

Ultrasonic or mechanical shear force is used as a stirring method. In order to homogenize the particle size, extruder treatment or microfluidizer treatment can be performed through a filter with a certain pore size. A multivesicular liposome containing a plurality of unilamellar liposomes can be separated into a plurality of unilamellar liposomes by extruder treatment or the like.

The liquid temperature of the mixture can be adjusted as appropriate, but is preferably set to a temperature higher than the phase transition temperature of lecithin contained in the mixture, for example, 35°C to 70°C.

In the production of the cosmetic liposome composition, the organic solvent and water may be evaporated from the liposome-containing aqueous solution.
The evaporation includes both intentional evaporation of part or all of the organic solvent and water, and spontaneous evaporation of part or all of the organic solvent and water during the stirring and emulsification process. be

In the case of intentionally evaporating the organic solvent and water, the evaporation method is not particularly limited. A method of stirring, a method of performing vacuum degassing, and the like can be mentioned.

The method of encapsulating the cosmetic ingredients in the liposomes is not particularly limited, and can be carried out by using an aqueous phase composition in which the cosmetic ingredients are dissolved at the time of emulsification.

The obtained liposome composition for cosmetics may be subjected to a dialysis method, a filtration method, an extrusion treatment method, or the like, whereby the liposomes contained therein can have a uniform average particle size. The extrusion treatment method is a method in which a cosmetic liposome composition is passed through a filter having pores to apply a physical shearing force to atomize the composition. When the liposome composition for cosmetics is passed through, the liposome composition for cosmetics and the filter can be quickly atomized by keeping the temperature higher than the phase transition temperature of lecithin.

(Application)
In one embodiment, the cosmetic liposomal composition of the present disclosure is a cosmetic. The form of the cosmetic is not particularly limited, and may be liquid, jelly-like, gel-like, cream-like, or solid such as a stick. It may be in a semi-solid state. Examples of cosmetics include skin care cosmetics (toner, milky lotion, serum, sunscreen (sunscreen agent), etc.), body cosmetics (body lotion, etc.), scalp cosmetics, packs, etc. It is not limited to these.

All publications, patent applications and technical standards mentioned herein are expressly incorporated herein by reference to the same extent as if each individual publication, patent application and technical standard were specifically and individually noted to be incorporated by reference. incorporated herein by reference.

EXAMPLES Hereinafter, the above-described embodiment will be specifically described with reference to Examples, but the above-described embodiment is not limited to these Examples.

<Example 1-1>
(Preparation of oil phase composition)
Hydrogenated soybean phospholipid (hydrogenated lecithin, PC content of 90% or more), polyoxyethylene phytosterol A (average number of added moles of oxyethylene group: 20), cholesterol, absolute ethanol and ascorbyl tetra-2-hexyldecanoate are mixed. , and dissolved by heating at 60°C for 10 minutes to obtain an oil phase composition A.

(Preparation of aqueous phase composition)
Anhydrous caffeine (beauty ingredient), nicotinic acid amide, disodium hydrogen phosphate anhydrous, sodium dihydrogen phosphate and purified water are mixed and dissolved by heating at 60 ° C. for 10 minutes to form an aqueous phase composition A. Obtained.

(Production of cosmetic liposome composition)
After the aqueous phase composition A obtained above was stirred with a homomixer while being kept at 60° C. (3400 rpm (revolutions per minute)), the oil phase composition A was added to the aqueous phase composition A.
After the addition, stirring was continued for 45 minutes at 3400 rpm. After stirring, the mixture was cooled to 35°C or lower, and filtered through a 230-mesh filter cloth after cooling to obtain a cosmetic liposome composition.
Table 1 shows the content of each component in the cosmetic liposome composition.
In the cosmetic liposome composition obtained in Example 1-1, the ratio of the polyoxyethylene phytosterol content to the ethanol content (polyoxyethylene phytosterol content/ethanol content) was , 0.002.
Further, in the liposome composition for cosmetics obtained in Example 1-1, the ratio of the polyoxyethylene phytosterol content to the cholesterol content (polyoxyethylene phytosterol content/cholesterol content) was By reference, it was 0.2.
In Table 1, the polyoxyethylene phytosterol content/ethanol content is indicated by "P/E", and the polyoxyethylene phytosterol content/cholesterol content is indicated by "P/C".
Tables 1 to 3 also show the content, P/E and P/C of each component in the following examples.

<Examples 1-2 to 1-4 and Comparative Examples 1-1 to 1-5>
A liposome composition for cosmetics was produced in the same manner as in Example 1-1, except that the composition of the liposome composition for cosmetics was changed as shown in Table 1.
In addition, in Comparative Example 1-1, liposome formation was not possible, so in Table 1, the subsequent evaluation results are marked with "-".

<Example 1-5>
(Preparation of oil phase composition)
Hydrogenated soybean phospholipid (hydrogenated lecithin, PC content of 90% or more), polyoxyethylene phytosterol A (average number of added moles of oxyethylene groups: 20), cholesterol, oleic acid, anhydrous ethanol, and ascorbyl tetra-2-hexyldecanoate were mixed and dissolved by heating at 60° C. for 10 minutes to obtain an oil phase composition B.

(Preparation of aqueous phase composition)
Pyridoxine hydrochloride (beauty ingredient), nicotinamide (beauty ingredient), disodium hydrogen phosphate anhydrous, sodium hydroxide solution (caustic soda) and purified water are mixed and dissolved by heating at 60°C for 10 minutes. Phase composition B was obtained.

(Production of liposome composition)
After the aqueous phase composition B obtained above was stirred with a homomixer while being kept at 60° C. (3000 rpm), the oil phase composition B was added to the aqueous phase composition B.
Stirring was continued at 3000 rpm for 45 minutes after the addition. After stirring, the mixture was cooled to 35° C. or lower, filtered through a 230-mesh filter cloth, and ethanol was evaporated using an evaporator to obtain a cosmetic liposome composition.
The content of each component in Table 1 is the content in the cosmetic liposome composition after ethanol evaporation.

<Examples 1-6 to 1-7>
A liposome composition for cosmetics was produced in the same manner as in Example 1-5, except that the composition of the liposome composition for cosmetics was changed as shown in Table 1.

<Examples 2-1 to 2-3>
(Preparation of oil phase composition)
Hydrogenated soybean phospholipid (hydrogenated lecithin, PC content of 90% or more), polyoxyethylene phytosterol B (average number of added moles of oxyethylene group: 30), cholesterol, absolute ethanol and ascorbyl tetra-2-hexyldecanoate are mixed. was dissolved by heating at 60° C. for 10 minutes to obtain an oil phase composition C1.

(Preparation of aqueous phase composition)
Anhydrous caffeine (beauty ingredient), nicotinic acid amide (beauty ingredient), disodium hydrogen phosphate anhydrous, sodium dihydrogen phosphate and purified water are mixed and dissolved by heating at 60°C for 10 minutes to form an aqueous phase. A composition C was obtained.

(Production of cosmetic liposome composition)
After the aqueous phase composition C obtained above was stirred with a homomixer while being kept at 60° C. (3400 rpm), the oil phase composition C1 was added to the aqueous phase composition C.
After the addition, stirring was continued for 45 minutes at 3400 rpm. After stirring, the mixture was cooled to 35°C or lower, and filtered through a 230-mesh filter cloth after cooling to obtain a cosmetic liposome composition.

<Example 2-4>
(Preparation of oil phase composition)
Hydrogenated soybean phospholipid (hydrogenated lecithin, PC content of 90% or more), polyoxyethylene phytosterol C (average number of added moles of oxyethylene group: 10), cholesterol, absolute ethanol and ascorbyl tetra-2-hexyldecanoate are mixed. , and dissolved by heating at 60° C. for 10 minutes to obtain an oil phase composition C2.

(Preparation of aqueous phase composition)
Anhydrous caffeine (beauty ingredient), nicotinic acid amide (beauty ingredient), disodium hydrogen phosphate anhydrous, sodium dihydrogen phosphate and purified water are mixed and dissolved by heating at 60°C for 10 minutes to form an aqueous phase. A composition C was obtained.

(Production of cosmetic liposome composition)
After the aqueous phase composition C obtained above was stirred with a homomixer while being kept at 60° C. (3400 rpm), the oil phase composition C2 was added to the aqueous phase composition C.
After the addition, stirring was continued for 45 minutes at 3400 rpm. After stirring, the mixture was cooled to 35°C or lower, and filtered through a 230-mesh filter cloth after cooling to obtain a cosmetic liposome composition.

<Example 2-5>
(Preparation of oil phase composition)
Hydrogenated soybean phospholipid (hydrogenated lecithin, PC content of 90% or more), polyoxyethylene phytosterol D (average number of added moles of oxyethylene groups: 5), cholesterol, absolute ethanol and ascorbyl tetra-2-hexyldecanoate were mixed. , and dissolved by heating at 60°C for 10 minutes to obtain an oil phase composition C3.

(Preparation of aqueous phase composition)
Anhydrous caffeine (beauty ingredient), nicotinic acid amide (beauty ingredient), disodium hydrogen phosphate anhydrous, sodium dihydrogen phosphate and purified water are mixed and dissolved by heating at 60°C for 10 minutes to form an aqueous phase. A composition C was obtained.

(Production of cosmetic liposome composition)
After the aqueous phase composition C obtained above was stirred with a homomixer while being kept at 60°C (3400 rpm), the oil phase composition C3 was added to the aqueous phase composition C.
After the addition, stirring was continued for 45 minutes at 3400 rpm. After stirring, the mixture was cooled to 35°C or lower, and filtered through a 230-mesh filter cloth after cooling to obtain a cosmetic liposome composition.

<Example 3-1>
(Preparation of oil phase composition)
Hydrogenated soybean phospholipid (hydrogenated lecithin, PC content of 90% by mass or more), polyoxyethylene phytosterol A (average number of added moles of oxyethylene groups: 20), cholesterol, absolute ethanol, and ascorbyl tetra-2-hexyldecanoate are mixed. and dissolved by heating at 60° C. for 10 minutes to obtain an oil phase composition D.

(Preparation of aqueous phase composition)
Pyridoxine hydrochloride (beauty ingredient), anhydrous caffeine (beauty ingredient), nicotinamide (beauty ingredient), disodium hydrogen phosphate anhydrous, sodium dihydrogen phosphate and purified water are mixed and heated at 60°C for 10 minutes. By dissolving the above, an aqueous phase composition D-1 was obtained.
Further, methyl paraoxybenzoate and purified water were mixed and dissolved by heating at 60° C. for 10 minutes to obtain an aqueous phase composition D-2.

(Production of cosmetic liposome composition)
After the aqueous phase composition D-1 obtained above was stirred with a homomixer while being kept at 60° C. (3400 rpm), the oil phase composition D was added to the aqueous phase composition D-1.
After the addition, stirring was continued for 45 minutes at 3400 rpm. After stirring, aqueous phase composition D-2 was added, and the mixture was uniformly stirred for 5 minutes and cooled to 35°C or lower. After cooling, the mixture was filtered through a 230-mesh filter cloth to obtain a cosmetic liposome composition.

<Example 3-2>
(Preparation of oil phase composition)
Hydrogenated soybean phospholipid (hydrogenated lecithin, PC content of 90% by mass or more), polyoxyethylene phytosterol A (average number of added moles of oxyethylene groups: 20), cholesterol, oleic acid, anhydrous ethanol, and tetra-2-hexyldecanoic acid Ascorbyl was mixed and dissolved by heating at 60° C. for 10 minutes to obtain an oil phase composition E.

(Preparation of aqueous phase composition)
Pyridoxine hydrochloride (beauty ingredient), nicotinamide (beauty ingredient), disodium hydrogen phosphate anhydrous, sodium hydroxide solution (caustic soda) and purified water are mixed and dissolved by heating at 60°C for 10 minutes. Phase composition E-1 was obtained.
Further, methyl p-hydroxybenzoate and purified water were mixed and dissolved by heating at 60° C. for 10 minutes to obtain aqueous phase composition E-2.

(Production of cosmetic liposome composition)
After the aqueous phase composition E-1 obtained above was stirred with a homomixer while being kept at 60° C. (3000 rpm), the oil phase composition E was added to the aqueous phase composition E-1.
After the addition, stirring was continued for 45 minutes at 3000 rpm. After stirring, the aqueous phase composition E-2 was added, and the mixture was uniformly stirred for 5 minutes and cooled to 35°C or less. After cooling, the mixture was filtered through a 230-mesh filter cloth to obtain a cosmetic liposome composition.

<<Encapsulation rate evaluation-1 (percentage of liposomes having a single lamellar structure)>>
The liposomes contained in the cosmetic liposome compositions produced in Examples and Comparative Examples were subjected to TEM images at a magnification of 50,000 using a transmission electron microscope in an ice-embedded state.

By counting the total number of liposomes present within a circle with a radius of 2 μm and the total number of liposomes having a single-lamellar structure at three arbitrary points on the TEM image, the single-lamellar The average value of the ratio of the total number of liposomes having a structure was confirmed and evaluated based on the following evaluation criteria. Evaluation results are shown in Tables 1 to 3.
1 to 5 show TEM images of Example 1-2, Example 3-1, Example 3-2, Comparative Example 1-2 and Comparative Example 1-5. As for the total number of liposomes and the total number of liposomes having a single-lamellar structure, the circles shown in FIGS. 1 to 5 indicate an arbitrary circle obtained by counting the total number of liposomes and the total number of liposomes having a single-lamellar structure.
(Evaluation criteria)
A: The ratio of liposomes having a single lamellar structure was 70% or more.
B: The ratio of liposomes having a single lamellar structure was 50% or more and less than 70%.
C: The ratio of liposomes having a single lamellar structure was 30% or more and less than 50%.
D: The percentage of liposomes with a single lamellar structure was less than 30%.

<<Encapsulation rate evaluation-2 (average particle size of liposomes)>>
The liposome compositions for cosmetics produced in Examples and Comparative Examples were diluted to 10 times their mass with pure water, and the volume average particle size of the contained liposomes was measured using a concentrated particle size analyzer FPAR-1000 (Otsuka Electronics Co., Ltd. (manufactured) by the dynamic light scattering method. The volume average particle size was measured within 20 to 24 hours after the liposome composition for cosmetics was produced. Moreover, the temperature of the measurement environment was set to 25°C. Tables 1 to 3 show the measurement results.

<<Evaluation of stability over time>>
Cosmetic liposome compositions produced in Examples 1-5 to 1-7, Examples 2-1 to 2-5, Examples 3-1 to 3-2, and Comparative Example 1-4 The object was allowed to stand in a 50° C. environment for one week.
After standing, the liposome composition for cosmetics was diluted with pure water to 10-fold mass, and the volume average particle size of the liposomes contained in the liposome composition for cosmetics was measured by the method described above.
The ratio of the volume-average particle size of the liposomes contained in the liposome composition after standing to the volume-average particle size of the liposomes contained in the liposome composition for cosmetics immediately after production (liposomes contained in the liposome composition after standing volume-average particle size/volume-average particle size of liposomes contained in the liposome composition for cosmetics immediately after production) was determined, and the stability over time of the liposome composition for cosmetics was evaluated based on the following evaluation criteria. Evaluation results are shown in Tables 1 to 3.
In Comparative Examples 1-2 and 1-3, the percentage of liposomes having a single lamellar structure was small, and the encapsulation rate of cosmetic ingredients was not sufficient. , the evaluation result is "-".
In Comparative Example 1-5, since the evaluation of encapsulation rate was not sufficient, evaluation of stability over time was not performed, and in Table 1, the evaluation result is indicated as "-".
(Evaluation criteria)
A: The volume average particle size ratio was less than 1.1.
B: The volume average particle size ratio was 1.1 or more and less than 1.4.
C: The volume average particle size ratio was 1.4 or more.

From the results in Table 1, the liposome compositions obtained in Examples 1-1 to 1-7 were at least polyoxyethylene phytosterol and ethanol obtained in Comparative Examples 1-2 to 1-3. Compared to a liposome composition that does not contain one of them, the proportion of liposomes having a single lamellar structure is high, indicating that the encapsulation rate of cosmetic ingredients is excellent.
Further, from the results in Table 1, the liposome compositions obtained in Examples 1-1 to 1-7 were at least one of cholesterol and phytosterol other than polyoxyethylene phytosterol obtained in Comparative Example 1-5. As compared with the liposome composition containing liposomes having an average particle size of less than 100 nm without containing
In addition, the liposomes contained in the liposome compositions obtained in Examples 1-1 to 1-7 have a large average particle size and are excellent in encapsulation rate of cosmetic ingredients.
In Comparative Examples 1-2 to 1-3, the contents of the cosmetic ingredients were the same as in Examples 1-1 to 1-7, but the single lamella contained in the obtained liposome compositions was Since the proportion of structured liposomes is small, most of the cosmetic ingredients are contained in the external aqueous phase rather than the internal aqueous phase.
The liposome compositions obtained in Examples 1-1 to 1-7 are the liposome compositions obtained in Comparative Example 1-4, which do not contain polyoxyethylene phytosterol and contain liposomes having an average particle size of more than 350 nm. It can be seen that the stability over time is superior to that of .
In Comparative Example 1-1, since ethanol was not contained, it is assumed that the liposome formation was low.

From the results in Tables 2 and 3, the liposome compositions obtained in Examples 2-1 to 2-5 and Examples 3-1 to 3-2 had a large proportion of liposomes having a single lamellar structure. It can be seen that the encapsulation rate of the beauty ingredients is excellent.
In addition, the liposomes contained in the liposome compositions obtained in Examples 2-1 to 2-5 and Examples 3-1 to 3-2 had a large average particle size and were found to be excellent in encapsulation rate of cosmetic ingredients. I understand.
Furthermore, it can be seen that the liposome compositions obtained in Examples 2-1 to 2-5 and Examples 3-1 to 3-2 have excellent stability over time.

1 to 5, the liposome compositions obtained in the examples have a higher proportion of liposomes having a single lamellar structure and are superior in encapsulation rate of cosmetic ingredients compared to the liposome compositions obtained in the comparative examples. I understand. In addition, it can be seen from FIG. 5 that a large amount of disk particles are contained instead of liposomes.

Hereinafter, formulation examples when the cosmetic liposome composition of the present disclosure is a cosmetic are disclosed.

<Example 4-1: Cream>
A cream having the following composition was prepared by a conventional method (total amount 100% by mass).
(composition)
- Liposome composition of Example 3-1 2.0% by mass
・Hydrogenated polyisobutene 10.0% by mass
・ Dipotassium glycyrrhizinate 1.0% by mass
・ Vaseline 8.0% by mass
・Di(phytosteryl/octyldodecyl) lauroyl glutamate 12.0% by mass
・Dimer dilinoleic acid (phytosteryl/isostearyl/cetyl/stearyl/behenyl) 4.0% by mass
・ Dimethicone 6.0% by mass
・ 1,2-pentanediol 3.0% by mass
・ Dipropylene glycol 7.0% by mass
・ Concentrated glycerin 9.0% by mass
・Polyethylene glycol 6000 (weight average molecular weight: 6000) 1.0% by mass
・Sodium hyaluronate 0.5% by mass
・Behenyl alcohol 2.0% by mass
・ Polyglyceryl monostearate 3.0% by mass
・Tocopherol 0.5% by mass
・ Astaxanthin 0.2% by mass
・Water-soluble collagen 1.0% by mass
・Hydrolyzed collagen solution (derived from fish) 1.0% by mass
・ N-acetyl-L-hydroxyproline 1.0% by mass
・Citric acid 0.7% by mass
・Sodium citrate appropriate amount ・Phenoxyethanol 0.3% by mass
・Remainder of purified water

<Example 4-2: Cream>
A cream having the following composition was prepared by a conventional method (total amount 100% by mass).
(composition)
- Liposome composition of Example 3-2 2.0% by mass
・Hydrogenated polyisobutene 10.0% by mass
・ Dipotassium glycyrrhizinate 1.0% by mass
・ Vaseline 8.0% by mass
・Di(phytosteryl/octyldodecyl) lauroyl glutamate 12.0% by mass
・Dimer dilinoleic acid (phytosteryl/isostearyl/cetyl/stearyl/behenyl) 4.0% by mass
・ Dimethicone 6.0% by mass
・ 1,2-pentanediol 3.0% by mass
・ Dipropylene glycol 7.0% by mass
・ Concentrated glycerin 9.0% by mass
・Polyethylene glycol 6000 (weight average molecular weight: 6000) 1.0% by mass
・Sodium hyaluronate 0.5% by mass
・Behenyl alcohol 2.0% by mass
・ Polyglyceryl monostearate 3.0% by mass
・Tocopherol 0.5% by mass
・ Astaxanthin 0.2% by mass
・Water-soluble collagen 1.0% by mass
・Hydrolyzed collagen solution (derived from fish) 1.0% by mass
・ N-acetyl-L-hydroxyproline 1.0% by mass
・Citric acid 0.7% by mass
・Sodium citrate appropriate amount ・Phenoxyethanol 0.3% by mass
・Remainder of purified water

The creams obtained in Examples 4-1 and 4-2 have good properties such as feeling during use and fragrance, and the cosmetic liposome composition of the present disclosure is suitably used for preparing creams. I found that it can be done.

<Example 5-1: Lotion>
A lotion having the following composition was prepared by a conventional method (total amount: 100% by mass).
(composition)
- Liposome composition of Example 3-1 2.0% by mass
・ Arbutin 2.0% by mass
・ Dipotassium glycyrrhizinate 1.0% by mass
・ Dipropylene glycol 4.0% by mass
・Polyoxyethylene methyl glucoside 1.0% by mass
・ 1,3-butylene glycol 4.0% by mass
・Polyethylene glycol 1.0% by mass
・ Ethanol 2.0% by mass
・ Polyoxyethylene hydrogenated castor oil 0.2% by mass
(Average number of added moles of oxyethylene: 60)
・Phenoxyethanol 0.3% by mass
・Glycerin mono-2-ethylhexyl ether 0.2% by mass
・ Oryzanol 0.01% by mass
・ Polyoxyethylene phytosterol 0.03% by mass
(Nikkol (registered trademark) BPS-20 manufactured by Nikko Chemicals Co., Ltd.)
・ N-acetyl-L-hydroxyproline 1.0% by mass
・Water-soluble collagen 1.0% by mass
・Hydrolyzed collagen 1.0% by mass
・Seaweed extract 1.0% by mass
- Lycopene-containing emulsified composition (composition prepared by the method described later) 0.1% by mass
・ Magnesium L-ascorbyl phosphate 1.0% by mass
・Sodium hyaluronate 0.2% by mass
・Citric acid 1.0% by mass
・Sodium citrate appropriate amount ・Remainder of water

<Example 5-2: Lotion>
A lotion having the following composition was prepared by a conventional method (total amount: 100% by mass).
(composition)
- Liposome composition of Example 3-2 2.0% by mass
・ Arbutin 2.0% by mass
・ Dipotassium glycyrrhizinate 1.0% by mass
・ Dipropylene glycol 4.0% by mass
・Polyoxyethylene methyl glucoside 1.0% by mass
・ 1,3-butylene glycol 4.0% by mass
・Polyethylene glycol 1.0% by mass
・ Ethanol 2.0% by mass
・ Polyoxyethylene hydrogenated castor oil 0.2% by mass
(Average number of added moles of oxyethylene: 60)
・Phenoxyethanol 0.3% by mass
・Glycerin mono-2-ethylhexyl ether 0.2% by mass
・ Oryzanol 0.01% by mass
・ Polyoxyethylene phytosterol 0.03% by mass
(Nikkol (registered trademark) BPS-20 manufactured by Nikko Chemicals Co., Ltd.)
・ N-acetyl-L-hydroxyproline 1.0% by mass
・Water-soluble collagen 1.0% by mass
・Hydrolyzed collagen 1.0% by mass
・Seaweed extract 1.0% by mass
- Lycopene-containing emulsified composition (composition prepared by the method described later) 0.1% by mass
・ Magnesium L-ascorbyl phosphate 1.0% by mass
・Sodium hyaluronate 0.2% by mass
・Citric acid 1.0% by mass
・Sodium citrate appropriate amount ・Remainder of water

The lotions obtained in Examples 5-1 and 5-2 have good properties in terms of feeling of use, fragrance, etc., and the liposome composition for cosmetics of the present disclosure is suitable for preparing lotions. found that it can be used.

The lycopene-containing emulsified compositions used in Examples 5-1 and 5-2 were prepared by the following method.

<Preparation of lycopene-containing emulsified composition>
(Preparation of oil phase composition a)
The components having the following composition were weighed in a container and heated and mixed for 5 minutes while stirring on a hot plate at 150°C to obtain an oil phase composition a.
(composition)
・ Tomato oleoresin (lycopene content 6% by mass) 2.8 parts by mass (manufactured by Sunbright Co., Ltd., Lyc-O-Mato 6%)
- 0.3 parts by mass of diglyceryl monostearate (manufactured by Nikko Chemicals Co., Ltd., Nikkol (registered trademark) DGMS, HLB: 5.0)
- Mixture of glyceryl tricaprylate and glyceryl tricaprate 11.3 parts by mass (manufactured by Kao Corporation, Coconard (registered trademark) MT, HLB: 1)
・ Mixed tocopherol 0.6 parts by mass (Riken Vitamin Co., Ltd., Riken E Oil 800)

(Preparation of aqueous phase composition a)
The components of the following composition were weighed in a container and heated and mixed with stirring in a constant temperature bath at 70°C to obtain an aqueous phase composition a.
(composition)
・ Decaglyceryl oleate 10 parts by mass (manufactured by Nikko Chemicals Co., Ltd., Decaglyn 1-O)
・45 parts by mass of glycerin ・30 parts by mass of purified water

The aqueous phase composition a thus obtained was added to the oil phase composition a, stirred and mixed, and dispersed for a predetermined time using an ultrasonic homogenizer to obtain a coarse dispersion.
After that, using an ultra-high pressure emulsifier (manufactured by Sugino Machine Co., Ltd., Ultimizer), high pressure emulsification of 200 MPa was performed on the obtained crude dispersion, and a lycopene-containing emulsified composition (lycopene content: 0.17 mass %) were prepared.

The resulting lycopene-containing emulsified composition was diluted 1% by mass with Milli-Q water, and the particle size of the dispersed particles was measured using a particle size analyzer FPAR-1000 (manufactured by Otsuka Electronics Co., Ltd.). rice field.

<Example 6-1: Jelly-like serum>
A jelly-like beauty essence having the following composition was prepared by a conventional method (total amount: 100% by mass).
(composition)
- Liposome composition of Example 3-1 2.0% by mass
・ Haematococcus algae extract 0.1% by mass
・ Astaxanthin-containing emulsified composition 0.2% by mass
(Krill extract, composition prepared by the method described below)
- A mixture of ceramide III and ceramide VI 1.0% by mass
・Hydrolyzed collagen 1.0% by mass
・ Acetyl hydroxyproline 1.0% by mass
・Ethylhexylglycerin 0.1% by mass
・Oleic acid 0.5% by mass
・ 1,3-butylene glycol 1.0% by mass
・Glycerin 2.0% by mass
・Sucrose 0.1% by mass
・Polyglyceryl oleate-10 0.1% by mass
・ Polyglyceryl-2 stearate 0.1% by mass
・Phenoxyethanol 0.2% by mass
・ Collagen 1.0% by mass
・ Oryzanol 0.01% by mass
・ Polyoxyethylene phytosterol 0.03% by mass
(Nikkol (registered trademark) BPS-20 manufactured by Nikko Chemicals Co., Ltd.)
・ Polyoxyethylene hydrogenated castor oil 0.2% by mass
(Average number of added moles of oxyethylene: 60)
・ Centella asiatica extract 0.01% by mass
・ Lecithin 0.1% by mass
・Citric acid 1.0% by mass
・ Sodium citrate appropriate amount ・ Copolymer of PEG-240, decyltetradeceth-20 and HDI 1.5% by mass
・Damask rose flower oil trace amount ・Perfume trace amount ・Water remainder

<Example 6-2: Jelly-like serum>
A jelly-like beauty essence having the following composition was prepared by a conventional method (total amount: 100% by mass).
(composition)
- Liposome composition of Example 3-2 2.0% by mass
・ Haematococcus algae extract 0.1% by mass
・ Astaxanthin-containing emulsified composition 0.2% by mass
(Krill extract, composition prepared by the method described below)
- A mixture of ceramide III and ceramide VI 1.0% by mass
・Hydrolyzed collagen 1.0% by mass
・ Acetyl hydroxyproline 1.0% by mass
・Ethylhexylglycerin 0.1% by mass
・Oleic acid 0.5% by mass
・ 1,3-butylene glycol 1.0% by mass
・Glycerin 2.0% by mass
・Sucrose 0.1% by mass
・Polyglyceryl oleate-10 0.1% by mass
・ Polyglyceryl-2 stearate 0.1% by mass
・Phenoxyethanol 0.2% by mass
・ Collagen 1.0% by mass
・ Oryzanol 0.01% by mass
・ Polyoxyethylene phytosterol 0.03% by mass
(Nikkol (registered trademark) BPS-20 manufactured by Nikko Chemicals Co., Ltd.)
・ Polyoxyethylene hydrogenated castor oil 0.2% by mass
(Average number of added moles of oxyethylene: 60)
・ Centella asiatica extract 0.01% by mass
・ Lecithin 0.1% by mass
・Citric acid 1.0% by mass
・ Sodium citrate appropriate amount ・ Copolymer of PEG-240, decyltetradeceth-20 and HDI 1.5% by mass
・Damask rose flower oil trace amount ・Perfume trace amount ・Water remainder

The jelly-like beauty essences obtained in Examples 6-1 and 6-2 have good properties such as feeling of use and fragrance. It turned out that it can be used suitably for preparation.

The astaxanthin-containing emulsified compositions used in Examples 6-1 and 6-2 were prepared by the following method.

<Preparation of astaxanthin-containing emulsified composition>
(Preparation of aqueous phase composition b)
The following components were dissolved while heating at 70° C. for 1 hour to obtain an aqueous phase composition b.
(composition)
・ Sucrose stearate (HLB: 16) 33.0 g
・Decaglyceryl monooleate (HLB: 12) 67.0 g
・Glycerin 450.0g
・Pure water 300.0g

(Preparation of oil phase composition b)
The following components were dissolved while heating at 70° C. for 1 hour to obtain an oil phase composition b.
(composition)
・Krill extract 15.0g
・Mixed tocopherol 32.0g
(Riken E Oil 800, manufactured by Riken Vitamin Co., Ltd.)
・Medium chain fatty acid glyceride 93.0g
(manufactured by Kao Corporation, Coconard (registered trademark) MT)
・Lecithin 10.0g
(Resion (registered trademark) P, derived from soybeans, manufactured by Riken Vitamin Co., Ltd.)

While the aqueous phase composition B obtained above is kept at 70 ° C., it is stirred using a homogenizer (manufactured by SMT Co., Ltd., HP93) (10000 rpm), and then the oil phase composition b to the aqueous phase composition b. was added to obtain a pre-emulsified product.
The resulting pre-emulsified product was cooled to about 40° C., and high-pressure emulsification was performed at a pressure of 200 MPa using Ultimizer HJP-25005 (manufactured by Sugino Machine Co., Ltd.). Thereafter, the mixture was filtered through a microfilter having an average pore size of 1 μm to prepare an astaxanthin-containing emulsified composition (content of astaxanthin: 0.3% by mass).

The resulting astaxanthin emulsified composition was diluted to 1% by mass with Milli-Q water, and the particle size of the dispersed particles was measured using a particle size analyzer FPAR-1000 (manufactured by Otsuka Electronics Co., Ltd.). rice field.

<Example 7-1: Sunscreen agent>
A sunscreen agent having the following composition was prepared by a conventional method (total amount: 100% by mass).
(composition)
- Liposome composition of Example 3-1 2.0% by mass
・Cyclopentasiloxane 20.0% by mass
・ Dimethicone 10.0% by mass
・Titanium oxide 5.0% by mass
・t-butyl methoxybenzoylmethane 1.0% by mass
・ HXMT-100ZA (Taika, average primary particle size 15 nm) 6.0% by mass
・Aluminum hydroxide 1.0% by mass
・ Isostearic acid 0.5% by mass
・ Sorbitan sesquioleate 1.0% by mass
・ Dipotassium glycyrrhizinate 0.5% by mass
・ Phosphate-L-ascorbyl magnesium 0.1% by mass
・Krill extract 0.5% by mass
・Water-soluble collagen 1.0% by mass
・Citric acid 0.7% by mass
・Sodium citrate appropriate amount ・Tocopherol 0.5% by mass
・ Oryzanol 0.01% by mass
・ Polyoxyethylene phytosterol 0.03% by mass
(Nikkol (registered trademark) BPS-20 manufactured by Nikko Chemicals Co., Ltd.)
・ Polyoxyethylene hydrogenated castor oil 0.2% by mass
(Average number of added moles of oxyethylene: 60)
・ Centella asiatica extract 0.01% by mass
・ Lecithin 0.1% by mass
・Perfume: trace amount ・Methyl paraoxybenzoate 0.15% by mass
・Remaining water

<Example 7-2: Sunscreen agent>
A sunscreen agent having the following composition was prepared by a conventional method (total amount: 100% by mass).
(composition)
- Liposome composition of Example 3-2 2.0% by mass
・Cyclopentasiloxane 20.0% by mass
・ Dimethicone 10.0% by mass
・Titanium oxide 5.0% by mass
・t-butyl methoxybenzoylmethane 1.0% by mass
・ HXMT-100ZA (Taika, average primary particle size 15 nm) 6.0% by mass
・Aluminum hydroxide 1.0% by mass
・ Isostearic acid 0.5% by mass
・ Sorbitan sesquioleate 1.0% by mass
・ Dipotassium glycyrrhizinate 0.5% by mass
・ Phosphate-L-ascorbyl magnesium 0.1% by mass
・Krill extract 0.5% by mass
・Water-soluble collagen 1.0% by mass
・Citric acid 0.7% by mass
・Sodium citrate appropriate amount ・Tocopherol 0.5% by mass
・ Oryzanol 0.01% by mass
・ Polyoxyethylene phytosterol 0.03% by mass
(Nikkol (registered trademark) BPS-20 manufactured by Nikko Chemicals Co., Ltd.)
・ Polyoxyethylene hydrogenated castor oil 0.2% by mass
(Average number of added moles of oxyethylene: 60)
・ Centella asiatica extract 0.01% by mass
・ Lecithin 0.1% by mass
・Perfume: trace amount ・Methyl paraoxybenzoate 0.15% by mass
・Remaining water

The sunscreen agents obtained in Examples 7-1 and 7-2 have good properties such as feeling during use and fragrance. It turned out that it can be used suitably.

Claims (9)

a liposome containing polyoxyethylene phytosterol, lecithin, and at least one of cholesterol and phytosterols other than the polyoxyethylene phytosterol, and having an average particle size of 100 nm to 350 nm;
A liposome composition for cosmetics, comprising an internal aqueous phase containing water, ethanol and a cosmetic component and encapsulated in the liposome, and an external aqueous phase containing water and ethanol and dispersing the liposome. 2. The cosmetic liposomal composition according to claim 1, wherein the polyoxyethylene phytosterol has an average addition mole number of oxyethylene groups of 10 or more. 3. The liposome composition for cosmetics according to claim 1, wherein the content of said polyoxyethylene phytosterol relative to the liposome composition for cosmetics is 0.1% by mass or more. The liposome composition for cosmetics according to any one of claims 1 to 3, wherein the content of ethanol with respect to the total weight of the liposome composition for cosmetics is 0.2% by mass to 30% by mass. . Claims 1 to 1, wherein the content of at least one of the cholesterol and the phytosterol other than the polyoxyethylene phytosterol is 0.1% by mass to 0.5% by mass with respect to the total mass of the liposome composition for cosmetics. 5. The cosmetic liposome composition according to any one of 4. The liposome composition for cosmetics according to any one of claims 1 to 5, wherein the cosmetic ingredient is a water-soluble cosmetic ingredient. The cosmetic liposome composition according to any one of claims 1 to 6, wherein the cosmetic ingredient is at least one selected from caffeine, pyridoxine hydrochloride and nicotinamide. The liposome composition for cosmetics according to any one of claims 1 to 7, wherein the ratio of the total number of liposomes having a single lamella structure to the total number of liposomes contained in the liposome composition for cosmetics is 70% or more. thing. The liposome composition for cosmetics according to any one of claims 1 to 8, which is a cosmetic.

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