JP2022019347A - Analyzer - Google Patents

Abstract

To provide an analyzer at low cost which can easily analyze a plurality of materials on the basis of Raman scattered light.SOLUTION: An analyzer (1) includes: a plurality of optical filters (14-1 to 14-5) for selectively transmitting light with a longer wavelength or a shorter wavelength than a specific wavelength of light scattered by a sample (S) by emission of excitation light, the optical filters having different specific wavelengths; a plurality of light detectors (15-1 to 15-5) for detecting the intensity of light which has passed through the optical filters (14-1 to 14-5); and a control unit (17) for specifying a material included in the sample (S) on the basis of a signal output from the plurality of light detectors (15-1 to 15-5).SELECTED DRAWING: Figure 1

Description

The present invention relates to a technique for analyzing a substance based on Raman scattered light.

An analyzer including a spectroscope is widely used as a technique for analyzing a substance based on Raman scattered light. In such an analyzer, the spectrum of light scattered by the sample is measured using a spectroscope. Then, the wavelength of the Raman scattered light emitted by the sample is specified from the measured light spectrum, and the substance constituting the sample is specified from the wavelength of the Raman scattered light. However, such an analyzer has a problem that it is expensive because it requires a spectroscope.

Examples of the technology that may contribute to solving such a problem include the optical gas sensor described in Patent Document 1. In the optical gas sensor described in Patent Document 1, among the light scattered by the sample, the power of the light transmitted through the interference filter is detected to detect a specific substance contained in the gas without using a spectroscope. A configuration for measuring the concentration is adopted.

Japanese Unexamined Patent Publication No. 2013-167497 (published on August 29, 2013)

As described above, the analyzer including the spectroscope has a problem of being expensive. Further, the optical gas sensor described in Patent Document 1 has a problem that it is impossible to analyze a plurality of substances at the same time, and it is also difficult to perform analysis in order. This is because, in the optical gas sensor described in Patent Document 1, the interference filter must be replaced every time the substance for which the concentration is to be measured is changed.

One aspect of the present invention has been made in view of the above problems, and an object thereof is to inexpensively realize an analyzer capable of easily analyzing a plurality of substances based on Raman scattered light. ..

In order to solve the above problems, the analyzer according to one aspect of the present invention selectively selects the light on the long wavelength side or the short wavelength side of the specific wavelength among the light scattered by the sample by the irradiation of the excitation light. A plurality of optical filters having a specific wavelength different from each other, and a single or a plurality of optical detectors for detecting the intensity of light passing through any of the plurality of optical filters. A control unit for identifying a substance contained in the sample based on a signal output from the optical detector is provided.

According to one aspect of the present invention, it is possible to inexpensively realize an analyzer capable of easily analyzing a plurality of substances based on Raman scattered light.

It is a schematic diagram which shows the structure of the analyzer which concerns on one Embodiment of this invention. It is a schematic diagram which shows the 1st modification of the analyzer shown in FIG. It is a perspective view which shows the 2nd modification of the analyzer shown in FIG. It is a schematic diagram which shows the 3rd modification of the analyzer shown in FIG. It is a graph which shows the Example of the analyzer shown in FIG. 1, and is the graph which shows the spectrum of the light scattered by acetone, ethanol, cyclohexane, and methanol.

1. 1. Outline of Analytical Device The analyzer 1 according to the embodiment of the present invention is a device that analyzes a substance contained in a sample based on the light scattered by the sample by irradiation with excitation light. In addition to the excitation light, the light scattered by the sample includes Raman scattered light having a wavelength different from that of the excitation light. The difference between the wavelength of the excitation light and the wavelength of the Raman scattered light depends on the substance contained in the sample. Therefore, by measuring the wavelength of Raman scattered light, the substance contained in the sample can be identified. The Raman scattered light includes Stokes light having a longer wavelength than the excitation light and anti-Stokes light having a shorter wavelength. In the analyzer 1, the substance is analyzed based on the Stokes light having a higher power than the anti-Stokes light.

2. 2. Configuration of Analytical Device The configuration of the analyzer 1 will be described with reference to FIG. FIG. 1 is a schematic diagram showing the configuration of the analyzer 1.

As shown in FIG. 1, the analyzer 1 includes a light source 11, an optical system 12, an optical branch path 13, optical filters 14-1 to 14-5, and photodetectors 15-1 to 15-5. It includes an AD (analytical / digital) converter 16 and a control unit 17.

The light source 11 is configured to generate excitation light to irradiate the sample S. The wavelength of the excitation light generated by the light source 11 is a predetermined specific wavelength. In this embodiment, a laser diode is used as the light source 11.

The optical system 12 is configured to guide the excitation light generated by the light source 11 to the sample S and to guide the light scattered by the sample S to the optical branch path 13. In the present embodiment, as the optical system 12, an optical system composed of a mirror 121, a half mirror 122, an objective lens 123, and a condenser lens 124 is used. The excitation light generated by the light source 11 is (1) reflected by the mirror 121, (2) reflected by the half mirror 122, and (3) by the objective lens 123, as shown by the chain line in FIG. After being focused, it is incident on the sample S. On the other hand, the light scattered by the sample S is collimated by (1) the objective lens 123, passes through the half mirror 122, and (3) the condenser lens, as shown by the one-point chain line in FIG. After being focused at 124, it is incident on the optical branch path 13.

The optical branch path 13 is configured to branch the light scattered by the sample S. In the present embodiment, as the optical branch path 13, one large-diameter optical fiber 131 and a plurality of optical fiber 131 having an incident end fused to the exit end of the large-diameter optical fiber 131 (five in the present embodiment). An optical branch path composed of small-diameter optical fibers 132-1 to 132-5 is used. The light guided through the optical system 12 described above is incident on (1) the large-diameter optical fiber 131, (2) waveguideed through the large-diameter optical fiber 131, and (3) the large-diameter optical fiber 131 and the small-diameter optical fiber. It is branched at the fusion point with 132-1 to 132-5, is incident on each of (4) small-diameter optical fibers 132-1 to 132-5, and (5) small-diameter optical fibers 132-1 to 132-5. Each of them is waveguideed. The light guided through each of the small diameter optical fibers 132-i (i = 1, 2, 3, 4, 5) is incident on the corresponding optical filter 14-i. The large-diameter optical fiber 131 and the small-diameter optical fibers 132-1 to 132-5 may be connected via a fiber coupler.

The optical filter 14-i is configured to selectively pass light having a wavelength longer than the predetermined cut-on wavelength λi, or selectively passes light having a wavelength shorter than the predetermined cut-off wavelength λi. It is a configuration for passing through. In the present embodiment, as the optical filter 14-i, a long-pass filter that selectively passes light having a wavelength longer than the cut-on wavelength λi (a low-pass filter that selectively passes light having a frequency lower than the cut-off frequency ωi). ) Is used. The cut-on wavelengths λ1 to λ5 are different from each other and are defined to satisfy λ0 <λ1 <λ2 <λ3 <λ4 <λ5. Here, λ0 is the central wavelength of the excitation light emitted by the light source 11. The light that has passed through the optical filter 14-i is incident on the corresponding photodetector 15-i.

The photodetector 15-i is configured to convert the light that has passed through the optical filter 14-i into an electric signal according to its intensity. In this embodiment, an avalanche photodiode is used as the photodetector 15-i. The electric signal output from the photodetector 15-i is input to the AD converter 16. The analyzer 1 may include an amplifier configured by an operational amplifier or the like between each photodetector 15-i and the AD converter 16. In this case, the electric signal output from each photodetector 15-i is amplified by the amplifier and then input to the AD converter 16.

The AD converter 16 is configured to convert an electric signal output from the photodetectors 15-1 to 15-5 into a digital signal. The digital signal output from the AD converter 16 is input to the control unit 17. The control unit 17 is configured to identify the substance contained in the sample S based on the digital signal output from the AD converter 16. In the present embodiment, a computer including a processor and a memory is used as the control unit 17. The method of specifying the substance contained in the sample S from the intensity of the light passing through each optical filter 14-i will be described later instead of the reference drawing.

3. 3. Control unit processing The electrical signal output from each photodetector 15-i is the intensity of the light scattered by the sample S that has passed through the corresponding optical filter 14-i, that is, the cut-on wavelength λi. Represents the intensity Ii of light with a longer wavelength than. The control unit 17 identifies the substance contained in the sample S based on these intensities I1 to I5. An example of a method for specifying a substance contained in the sample S based on the intensities I1 to I5 is as follows.

Calculation step:
The control unit 17 has an intensity I1 to I5 of light represented by an electromagnetic signal output from the optical detectors 15-1 to 15-5, that is, an intensity I1 of light transmitted through each of the optical filters 14-1 to 14-5. Based on ~ I5, the calculation step of calculating the intensities J1 (S) to J15 (S) of the light belonging to each of the plurality of wavelength bands w1 to w15 in the light scattered by the sample S is executed. In the present embodiment, as the plurality of wavelength bands w1 to w15, 15 wavelength bands w1 to w15 determined from the five cut-on wavelengths λ1 to λ5 are used as shown in the table below. The method for calculating the intensity Jk (S) of the light belonging to each wavelength band wk (k = 1, 2, ..., 15) in the light scattered by the sample S is as shown in the table below.

Specific step:
Subsequently, the control unit 17 determines the intensities J1 (S) to J15 (S) calculated in the calculation step, that is, the light belonging to each of the plurality of wavelength bands w1 to w15 in the light scattered by the sample S. A specific step of identifying the material constituting the sample S is performed based on the intensities J1 (S) to J15 (S). In the present embodiment, the memory of the control unit 17 has the candidate substances Aj (j = 1, 2) for each of the plurality of predetermined candidate substances A1, A2, ..., Am (m is a natural number of 2 or more). , ..., The intensities J1 (Aj) to J15 (Aj) of the light belonging to each of the wavelength bands w1 to w15 in the light scattered in m) are stored. The processor of the control unit 17 uses the light intensities J1 (S) to J15 (S) calculated in the calculation step as the intensities J1 (Aj) to corresponding to each candidate substance Aj stored in the memory of the control unit 17. By comparing with J15 (Aj) 15, the substance constituting the sample S is specified. For example, of the intensities stored in the memory of the control unit 17, the correlation coefficient with the light intensities J1 (S) to J15 (S) calculated in the calculation step (more accurately, the sample correlation coefficient). If the intensity J1 (A1) to J15 (A1) corresponding to the candidate substance A1 is the largest (closest to 1), the substance contained in the sample S is specified as the candidate substance A1. Alternatively, among the intensities stored in the memory of the control unit 17, the intensity corresponding to the candidate substance A2 having the largest correlation coefficient with the light intensities J1 (S) to J15 (S) calculated in the calculation step. If it is J1 (A2) 1 to J15 (A1) 15, the substance contained in the sample S is specified as the candidate substance A2.

In this embodiment, a configuration is adopted in which the intensity Jk (S) of the light belonging to each wavelength band wk is calculated for all of the 15 wavelength bands w1 to w15 determined by the five cut-on wavelengths λ1 to λ5. However, the present invention is not limited to this. That is, a configuration may be adopted in which the intensity Jk (S) of the light belonging to each wavelength band wk is calculated for a part of the 15 wavelength bands w1 to w15 determined by the five cut-on wavelengths λ1 to λ5. For example, a configuration in which only the light intensities J1 (S), J2 (S), J3 (S), J4 (S), and J5 (S) belonging to the five wavelength bands w1, w2, w3, w4, and w5 are calculated. May be adopted. In this case, these intensities J1 (S), J2 (S), J3 (S), J4 (S), J5 (S) and the intensities J1 (Aj), J2 corresponding to each candidate substance Aj stored in the memory. (Aj), J3 (Aj), J4 (Aj), J5 (Aj) are compared. Alternatively, a configuration may be adopted in which only the light intensities J6 (S), J10 (S), J13 (S), and J15 (S) belonging to the four wavelength bands w6, w10, w13, and w15 are calculated. In this case, these intensities J6 (S), J10 (S), J13 (S), J15 (S) and the intensities J6 (Aj), J10 (Aj), J13 corresponding to each candidate substance Aj stored in the memory. (Aj) and J15 (Aj) are compared. Further, in this comparison, the intensities J1 (S) to J15 (S) calculated in the calculation step may be dimensionally compressed. Examples of the dimensional compression method include a method using principal component analysis and a method using Mahalanobis distance.

4. Effect of Analytical Device According to the analyzer 1 according to the present embodiment, it is possible to easily analyze a plurality of substances without the trouble of exchanging filters. Moreover, the analyzer 1 according to the present embodiment does not require a spectroscope for analyzing a plurality of substances. Therefore, the analyzer 1 according to the present embodiment can be realized at a lower cost than the conventional analyzer using a spectroscope.

5. Modification example of analyzer (modification example 1)
The analyzer 1 described above can be modified to include the optical branch path 13A instead of the optical branch path 13. FIG. 2 is a schematic diagram showing an optical branch path 13A.

The optical branch path 13A is composed of small-diameter optical fibers 132-1 to 132-5 in which the vicinity of the incident end is bundled, and is arranged on the optical path of the light emitted from the optical system 12. In the optical branch path 13A, the light emitted from the optical system 12 is input to the incident ends of the small-diameter optical fibers 132-1 to 132-5 without passing through the large-diameter optical fiber 131.

The analyzer 1 deformed in this way can more easily configure the optical branch path 13 and can be realized at a lower cost.

(Modification 2)
The analyzer 1 described above can be modified to include a combination of a turret 18 and a single photodetector 15 in place of the combination of the optical branch path 13 and the plurality of photodetectors 15-1 to 15-5. be. FIG. 3 is a perspective view showing a combination of the turret 18 and a single photodetector 15.

The turret 18 is a disk-shaped support that supports the optical filters 14-1 to 14-5, and is arranged on the optical path of the light emitted from the optical system 12. The turret 18 can rotate about an axis parallel to the optical axis of the light emitted from the optical system 12 as a rotation axis. By rotating the turret 18, it is possible to switch which of the optical filters 14-1 to 14-5 the light emitted from the optical system 12 is incident on. The light that has passed through any of the optical filters 14-1 to 14-5 is incident on the photodetector 15.

In the analyzer 1 according to this modification, the electric signal output from the light detector 15 when the light emitted from the optical system 12 is incident on the optical filter 14-i is the light scattered by the sample S. Of these, it represents the intensity Ii of light having a wavelength longer than the cut-on wavelength λi. The procedure for the control unit 17 to specify the substance contained in the sample S based on these intensities I1 to I5 is the same as the procedure described above.

(Modification 3)
The above-mentioned analyzer 1 can be modified to include a rotating mirror 19 instead of the optical branch path 13. FIG. 4 is a schematic view showing the rotary mirror 19.

The rotating mirror 19 is arranged on the optical path of the light emitted from the optical system 12. The rotary mirror 19 can rotate about an axis perpendicular to the optical axis of the light emitted from the optical system 12 as a rotation axis. By rotating the rotary mirror 19, it is possible to switch which of the optical filters 14-1 to 14-5 the light emitted from the optical system 12 is incident on. The light that has passed through each optical filter 14-i is incident on the corresponding photodetector 15-i.

In the analyzer 1 according to this modification, an electric signal output from the photodetector 15-i when the light emitted from the optical system 12 and reflected by the rotating mirror 19 is incident on the optical filter 14-i. Represents the intensity Ii of the light having a wavelength longer than the cut-on wavelength λi among the light scattered by the sample S. The procedure for the control unit 17 to specify the substance contained in the sample S based on these intensities I1 to I5 is the same as the procedure described above.

(Modification example 4)
The above-mentioned analyzer 1 can be modified to use optical filters 14-1 to 14-5 having cut-on wavelengths λ1 to λ5 smaller than the center wavelength λ0. In this case, the bands w1 to w4 divided by the cut-on wavelengths λ1 to λ5 are lower wavelength bands than the center wavelength λ0 of the excitation light. Therefore, the control unit 17 identifies the substance contained in the sample S based on the anti-Stokes light.

(Modification 5)
The above-mentioned analyzer 1 can be modified to use both optical filters 14-1 to 14-5 having cut-on wavelengths λ1 to λ5 smaller than and larger than the center wavelength λ0. In this case, at least one of the bands w1 to w4 divided by the cut-on wavelengths λ1 to λ5 is a band lower than the center wavelength λ0 of the excitation light, and at least one of them is the center wavelength λ0 of the excitation light. The band is on the longer wavelength side. Therefore, the control unit 17 identifies the substance contained in the sample S based on both the anti-Stokes light and the Stokes light.

(Modification 6)
The above-mentioned analyzer 1 selectively passes light having a wavelength shorter than the cutoff wavelength λ'i as an optical filter 14-i (a short pass filter having a frequency higher than the cutoff frequency ω'i). It can be modified to use a high-pass filter). The cutoff wavelengths λ'1 to λ'5 are different from each other and are set to satisfy λ'0 <λ'1 <λ'2 <λ'3 <λ'4 <λ'5. Here, λ0 is the central wavelength of the excitation light emitted by the light source 11. The control unit 17 belongs to each wavelength band w'k (k = 1, 2, ..., 15) for the 15 wavelength bands w'1 to w'15 determined by the cutoff wavelengths λ'1 to λ'5. The light intensity Jk (S) is calculated. The table below lists the definitions of each wavelength band w'k and the calculation method of the light intensity J'k belonging to each wavelength band w'k.

(Modification 7)
The above-mentioned analyzer 1 can be modified so that the control unit 17 identifies the substance contained in the sample S by using the trained model generated by machine learning. In this case, the memory of the control unit 17 stores, for example, a trained model in which the intensities I1 to I5 of the light passing through the optical filters 14-1 to 14-5 are input and the substance contained in the sample S is output. .. In this case, the processor of the control unit 17 identifies the substance contained in the sample S by using this trained model. It should be noted that such a trained model can be generated by supervised learning, unsupervised learning, or anti-supervised learning.

6. Example An embodiment of the analyzer 1 will be described with reference to FIG. In this example, acetone, ethanol, cyclohexane, and methanol are used as candidate substances A1, A2, A3, and A4, and sample S is specified as a candidate substance. FIG. 5 is a graph showing the spectrum of light scattered by acetone, ethanol, cyclohexane, and methanol when irradiated with excitation light having a wavelength of 532 nm.

In this embodiment, first, λ1 is set to 550 nm, λ2 is set to 570 nm, λ3 is set to 630 nm, λ4 is set to 650 nm, and λ5 is set to 710 nm. The light intensities J1 (Aj) to J15 (Aj) to which the light belongs were calculated based on the spectrum shown in FIG. 5 and stored in the memory of the control unit 17. In the calculation, (1) the intensity of the wavelength of 800 nm or more was ignored, and (2) the intensities J1 (candidate substance) to J15 (candidate substance) for each candidate substance were standardized by the maximum intensity for the candidate substance. .. The calculation results are shown in the table below.

Next, using ethanol as the sample S, the sample S was irradiated with excitation light having a wavelength of 532 nm. Then, from the light intensities I1 to I5 detected by the photodetectors 15-1 to 15-5, the light intensities J1 (S) to J15 (S) belonging to the 15 wavelength bands w1 to w15 according to Table 1. Was calculated. In the calculation, (1) the intensity of the wavelength of 800 nm or more was ignored, and (2) the intensities J1 to J15 were standardized at the maximum intensity. The calculation results are shown in the table below.

Next, the correlation coefficient between the intensities J1 (S) to J15 (S) for the sample S shown in Table 4 and the intensities J1 (Aj) to J15 (Aj) for each candidate substance shown in Table 3 was calculated. The calculation results are shown in the table below.

As is clear from Table 5, the candidate substance having the highest correlation between the sample S and the intensities J1 (S) to J15 (S) is ethanol. Therefore, the control unit 17 determined that the sample S was ethanol. That is, the control unit 17 correctly identified the substance constituting the sample S.

7. Appendix In the above-described embodiment and each modification, the control unit 17 is realized by a logic circuit (hardware) formed in an integrated circuit (IC chip) or the like instead of being configured by a computer including a memory and a processor. May be good.

When the control unit 17 is configured by a computer including a memory and a processor, a CPU (Central Processing Unit) can be used as the processor, for example. As the memory, in addition to a "non-temporary tangible medium" such as a ROM (Read Only Memory), a tape, a disk, a card, a semiconductor memory, a programmable logic circuit, or the like can be used. Further, a RAM (Random Access Memory) for expanding the above program may be further provided. Further, the program may be supplied to the computer via any transmission medium (communication network, broadcast wave, etc.) capable of transmitting the program. It should be noted that one aspect of the present invention can also be realized in the form of a data signal embedded in a carrier wave, in which the above program is embodied by electronic transmission.

The present invention is not limited to the above-described embodiments, and various modifications can be made within the scope of the claims, and the embodiments obtained by appropriately combining the technical means disclosed in the different embodiments. Is also included in the technical scope of the present invention.

8. Summary The analyzer according to the present embodiment is a plurality of optical filters that selectively pass light on the long wavelength side or the short wavelength side of a specific wavelength among the light scattered by the sample by irradiation with the excitation light. , A plurality of optical filters having different specific wavelengths, a single or a plurality of optical detectors for detecting the intensity of light passing through any of the plurality of optical filters, and a signal output from the optical detector. Based on the above, the control unit for identifying the substance contained in the sample is provided.

According to the above configuration, it is possible to easily analyze a plurality of substances without the trouble of replacing the filter. Moreover, according to the above configuration, a spectroscope is not required to analyze a plurality of substances. Therefore, according to the above configuration, it can be realized at a lower cost than a conventional analyzer using a spectroscope.

Further, in the analyzer according to the present embodiment, it is possible to adopt a configuration further including an optical branch path that branches the light scattered by the sample and causes the light to be incident on the plurality of optical filters.

According to the above configuration, the light scattered by the sample can be efficiently guided to a plurality of optical filters.

Further, in the analyzer according to the present embodiment, the photodetector is a single unit, and a turret for switching which of the plurality of optical filters the light scattered by the sample is incident on is further provided. It is possible to adopt the provided configuration.

According to the above configuration, the light scattered by the sample can be efficiently guided to any of a plurality of optical filters. Moreover, since there is only one photodetector, the analyzer can be realized at a lower cost.

Further, in the analyzer according to the present embodiment, the photodetector is a plurality, and a rotating mirror for switching which of the plurality of optical filters the light scattered by the sample is incident on is further provided. It is possible to adopt the provided configuration.

According to the above configuration, the light scattered by the sample can be efficiently guided to any of a plurality of optical filters.

Further, in the analyzer according to the present embodiment, the control unit is the intensity of light represented by the signal output from the optical detector, and is based on the intensity of light transmitted through each of the plurality of optical filters. The calculation step of calculating the intensity of the light belonging to each of the plurality of wavelength bands in the light scattered by the sample, and the light intensity calculated by the calculation step, which are scattered by the sample. It is possible to employ a configuration that performs a specific step of identifying a substance contained in the sample based on the intensity of the light belonging to each of the plurality of wavelength bands in the light.

According to the above configuration, the substance constituting the sample can be specified with high accuracy.

Further, in the analyzer according to the present embodiment, in the memory of the control unit, light belonging to each of the plurality of wavelength bands in the light scattered by the candidate substances is stored in the memory of the control unit. The intensity of the light belonging to each of the plurality of wavelength bands in the light scattered by the sample is stored, and the control unit determines the intensity of the light belonging to each of the plurality of wavelength bands in the light scattered by the sample, and the plurality of wavelengths in the light scattered by each candidate substance. By comparing with the intensity of light belonging to each of the bands, it is possible to adopt a configuration that identifies whether the substance contained in the sample is one of the plurality of candidate substances.

According to the above configuration, it is possible to accurately identify which of the plurality of predetermined candidate substances the substance constituting the sample is.

1 Analytical device 11 Light source 12 Optical system 13 Optical branch path 14-1 to 14-5 Optical filter 15-1 to 15-5 Photodetector 16 AD converter 17 Control unit 18 Turret 19 Rotating mirror S sample

Claims (6)

A plurality of optical filters that selectively pass light on the long wavelength side or the short wavelength side of a specific wavelength among the light scattered by the sample by irradiation with the excitation light, and the plurality of optical filters having different specific wavelengths from each other. With a filter,
A single or multiple photodetectors that detect the intensity of light that has passed through any of the plurality of optical filters.
A control unit for identifying a substance contained in the sample based on a signal output from the photodetector is provided.
An analyzer characterized by that. The analyzer further comprises an optical branch path that branches the light scattered by the sample and causes it to enter the plurality of optical filters.
The analyzer according to claim 1. The photodetector is single and
The analyzer further comprises a turret for switching which of the plurality of optical filters the light scattered by the sample is incident on.
The analyzer according to claim 1. There are a plurality of photodetectors, and there are a plurality of photodetectors.
The analyzer further comprises a rotating mirror for switching which of the plurality of optical filters the light scattered by the sample is incident on.
The analyzer according to claim 1. The control unit is the intensity of the light represented by the signal output from the optical detector, and is a plurality of light scattered in the sample based on the intensity of the light transmitted through each of the plurality of optical filters. The calculation step of calculating the intensity of light belonging to each of the wavelength bands of the above, and the intensity of light calculated in the calculation step, which belongs to each of the plurality of wavelength bands in the light scattered by the sample. Performing specific steps to identify the substances contained in the sample based on the intensity of the light.
The analyzer according to any one of claims 1 to 4, wherein the analyzer is characterized by the above. The memory of the control unit stores the intensities of light belonging to each of the plurality of wavelength bands in the light scattered by the plurality of predetermined candidate substances.
The control unit determines the intensity of light belonging to each of the plurality of wavelength bands in the light scattered by the sample, and the intensity of light belonging to each of the plurality of wavelength bands in the light scattered by each candidate substance. By comparing with, it is specified whether the substance contained in the sample is one of the plurality of candidate substances.
The analyzer according to claim 5.

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