JP2022001040A5 - - Google Patents
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- JP2022001040A5 JP2022001040A5 JP2021101351A JP2021101351A JP2022001040A5 JP 2022001040 A5 JP2022001040 A5 JP 2022001040A5 JP 2021101351 A JP2021101351 A JP 2021101351A JP 2021101351 A JP2021101351 A JP 2021101351A JP 2022001040 A5 JP2022001040 A5 JP 2022001040A5
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- differential image
- slope
- differential
- index value
- histogram
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（第６項）第１項～第５項のいずれか１項に記載の細胞の評価方法では、
前記位相像から画素毎の空間的な微分値を求め微分画像を作成する微分画像作成ステップと、
前記微分画像において目的細胞に対応する範囲に含まれる各画素の信号値の出現頻度分布を示すヒストグラムを作成し、該ヒストグラムに現れるピークの高信号値側のスロープの勾配の程度を数値化した指標値を算出する第２の指標値算出ステップと、
をさらに有するものとすることができる。
(Section 6) In the cell evaluation method described in any one of Items 1 to 5,
a differential image creation step of determining a spatial differential value for each pixel from the phase image and creating a differential image;
Create a histogram showing the appearance frequency distribution of signal values of each pixel included in the range corresponding to the target cell in the differential image, and quantify the degree of slope of the slope on the high signal value side of the peak appearing in the histogram. a second index value calculation step of calculating the index value obtained by
It may further have the following.
（第１３項）また第８項～第１２項のいずれか１項に記載の細胞解析装置では、
前記位相像から画素毎の空間的な微分値を求め微分画像を作成する微分画像作成部と、
前記微分画像において目的細胞に対応する範囲に含まれる各画素の信号値の出現頻度分布を示すヒストグラムを作成し、該ヒストグラムに現れるピークの高信号値側のスロープの勾配の程度を数値化した指標値を算出する第２の指標値算出部と、をさらに備えるものとすることができる。
(Section 13) Furthermore, in the cell analysis device according to any one of Sections 8 to 12,
a differential image creation unit that calculates a spatial differential value for each pixel from the phase image and creates a differential image;
Create a histogram showing the appearance frequency distribution of signal values of each pixel included in the range corresponding to the target cell in the differential image, and quantify the degree of slope of the slope on the high signal value side of the peak appearing in the histogram. The display device may further include a second index value calculation unit that calculates the index value.
Claims (2)
前記微分画像において目的細胞に対応する範囲に含まれる各画素の信号値の出現頻度分布を示すヒストグラムを作成し、該ヒストグラムに現れるピークの高信号値側のスロープの勾配の程度を数値化した指標値を算出する第２の指標値算出ステップと、
をさらに有する、請求項１～５のいずれか１項に記載の細胞の評価方法。
a differential image creation step of determining a spatial differential value for each pixel from the phase image and creating a differential image;
Create a histogram showing the appearance frequency distribution of signal values of each pixel included in the range corresponding to the target cell in the differential image, and quantify the degree of slope of the slope on the high signal value side of the peak appearing in the histogram. a second index value calculation step of calculating the index value obtained by
The method for evaluating cells according to any one of claims 1 to 5, further comprising:
前記微分画像において目的細胞に対応する範囲に含まれる各画素の信号値の出現頻度分布を示すヒストグラムを作成し、該ヒストグラムに現れるピークの高信号値側のスロープの勾配の程度を数値化した指標値を算出する第２の指標値算出部と、をさらに備える、請求項８～１２のいずれか１項に記載の細胞解析装置。
a differential image creation unit that calculates a spatial differential value for each pixel from the phase image and creates a differential image;
Create a histogram showing the appearance frequency distribution of signal values of each pixel included in the range corresponding to the target cell in the differential image, and quantify the degree of slope of the slope on the high signal value side of the peak appearing in the histogram. The cell analysis device according to any one of claims 8 to 12, further comprising a second index value calculation unit that calculates the index value determined by the cell analysis method.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020106390 | 2020-06-19 | ||
| JP2020106390 | 2020-06-19 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2022001040A JP2022001040A (en) | 2022-01-06 |
| JP2022001040A5 true JP2022001040A5 (en) | 2023-10-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| JP2021101351A Pending JP2022001040A (en) | 2020-06-19 | 2021-06-18 | Evaluation method of cell and cell analysis apparatus |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023195491A1 (en) * | 2022-04-06 | 2023-10-12 | 富士フイルム株式会社 | Image capture system and culture condition adjustment method |
| WO2023248954A1 (en) * | 2022-06-24 | 2023-12-28 | ソニーグループ株式会社 | Biological specimen observation system, biological specimen observation method, and dataset creation method |
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2021
- 2021-06-18 JP JP2021101351A patent/JP2022001040A/en active Pending
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