JP2021522857A - 標的細菌を死滅させるための方法および組成物 - Google Patents

標的細菌を死滅させるための方法および組成物 Download PDF

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JP2021522857A
JP2021522857A JP2021510294A JP2021510294A JP2021522857A JP 2021522857 A JP2021522857 A JP 2021522857A JP 2021510294 A JP2021510294 A JP 2021510294A JP 2021510294 A JP2021510294 A JP 2021510294A JP 2021522857 A JP2021522857 A JP 2021522857A
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bacteriophage
crispr
cas system
target
protein
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JP2021522857A5 (https=
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エム． ガロフォロ，ポール
エム． ガロフォロ，ポール
ジー． オースターアウト，デビッド
ジー． オースターアウト，デビッド
セル，カート
ウォン，サンディ
ヒューイット テュソン，ハンナ
ヒューイット テュソン，ハンナ
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ローカス バイオサイエンシーズ，インク．
ローカス バイオサイエンシーズ，インク．
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JP2021510294A 2018-05-04 2019-05-03 標的細菌を死滅させるための方法および組成物 Pending JP2021522857A (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201862667400P 2018-05-04 2018-05-04
US62/667,400 2018-05-04
US201862743740P 2018-10-10 2018-10-10
US62/743,740 2018-10-10
US201962818066P 2019-03-13 2019-03-13
US62/818,066 2019-03-13
PCT/US2019/030695 WO2019213592A1 (en) 2018-05-04 2019-05-03 Methods and compositions for killing a target bacterium

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JP2021522857A true JP2021522857A (ja) 2021-09-02
JP2021522857A5 JP2021522857A5 (https=) 2022-05-13

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KR (1) KR20210018273A (https=)
CN (1) CN112424363A (https=)
AU (1) AU2019262200A1 (https=)
CA (1) CA3099316A1 (https=)
TW (1) TW202014519A (https=)
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Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3711488A1 (en) 2015-05-06 2020-09-23 Snipr Technologies Limited Altering microbial populations & modifying microbiota
GB201609811D0 (en) 2016-06-05 2016-07-20 Snipr Technologies Ltd Methods, cells, systems, arrays, RNA and kits
US10760075B2 (en) 2018-04-30 2020-09-01 Snipr Biome Aps Treating and preventing microbial infections
FR3096373B1 (fr) * 2019-05-24 2024-09-13 Ifp Energies Now Outil genetique optimisé pour modifier les bacteries
US20220265766A1 (en) * 2019-07-30 2022-08-25 The Regents Of The University Of California Antimicrobial therapy through the combination of pore-forming agents and histones
WO2021092254A1 (en) * 2019-11-06 2021-05-14 Locus Biosciences, Inc. Phage compositions comprising crispr-cas systems and methods of use thereof
US20230002761A1 (en) * 2019-11-19 2023-01-05 Benson Hill, Inc. Anti-bacterial crispr compositions and methods
CA3171941A1 (en) * 2020-03-16 2021-09-23 Joseph E. Peters Compositions and methods comprising improved guide rnas
WO2021195594A1 (en) * 2020-03-26 2021-09-30 San Diego State University (SDSU) Foundation, dba San Diego State University Research Foundation Compositions and methods for treating or ameliorating infections
WO2021231689A1 (en) * 2020-05-14 2021-11-18 Chan Zuckerberg Biohub, Inc. Phage-mediated delivery of genes to gut microbiome
GB202007943D0 (en) * 2020-05-27 2020-07-08 Snipr Biome Aps Products & methods
CA3197277A1 (en) * 2020-11-05 2022-05-12 Paul GAROFOLO Phage compositions for escherichia comprising crispr-cas systems and methods of use thereof
US20240011041A1 (en) * 2020-11-05 2024-01-11 Locus Biosciences, Inc. Phage compositions for pseudomonas comprising crispr-cas systems and methods of use thereof
SK289101B6 (sk) * 2020-12-17 2023-08-09 Ústav molekulárnej biológie Slovenskej akadémie vied, verejná výskumná inštitúcia Antimikrobiálny proteín, antimikrobiálny rekombinantný proteín s lytickými vlastnosťami, expresný vektor, spôsob ich prípravy a použitie
WO2022235816A2 (en) * 2021-05-05 2022-11-10 Locus Biosciences, Inc. Bacteriophage comprising type i crispr-cas systems
WO2022235799A2 (en) * 2021-05-05 2022-11-10 Locus Biosciences, Inc. Phage compositions for staphylococcus comprising crispr-cas systems and methods of use thereof
GB202209518D0 (en) 2022-06-29 2022-08-10 Snipr Biome Aps Treating & preventing E coli infections
EP4604969A1 (en) * 2022-10-17 2025-08-27 Locus Biosciences, Inc. Staphylococcus phage compositions and cocktails thereof
CN117210413B (zh) * 2023-08-04 2026-04-21 湖北文理学院 纹带棒状杆菌噬菌体及其应用
WO2025109112A1 (en) * 2023-11-22 2025-05-30 Centre National De La Recherche Scientifique Engineered phagemids and methods for antibacterial peptide delivery against bacterial pathogens in particular clostridioides difficile
US12274722B1 (en) * 2024-04-24 2025-04-15 Phage Technology Center Gmbh Antibiotic free treatment of mastitis

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016177682A1 (en) * 2015-05-06 2016-11-10 Snipr Technologies Limited Altering microbial populations & modifying microbiota
WO2016205276A1 (en) * 2015-06-15 2016-12-22 North Carolina State University Methods and compositions for efficient delivery of nucleic acids and rna-based antimicrobials
JP2017513489A (ja) * 2014-04-14 2017-06-01 ネメシス バイオサイエンス リミティド 治療薬
JP2017536831A (ja) * 2014-11-26 2017-12-14 テクノロジー イノベーション モメンタム ファンド（イスラエル）リミテッド パートナーシップＴｅｃｈｎｏｌｏｇｙ Ｉｎｎｏｖａｔｉｏｎ Ｍｏｍｅｎｔｕｍ Ｆｕｎｄ（ｉｓｒａｅｌ）Ｌｉｍｉｔｅｄ Ｐａｒｔｎｅｒｓｈｉｐ 細菌遺伝子の標的化削減

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2341149T3 (en) * 2005-08-26 2017-02-27 Dupont Nutrition Biosci Aps Use of CRISPR-associated genes (Cas)
CN101688241B (zh) * 2007-03-02 2015-01-21 杜邦营养生物科学有限公司 具有改善的噬菌体抗性的培养物
HRP20200529T1 (hr) * 2014-06-06 2020-09-04 Regeneron Pharmaceuticals, Inc. Postupci i pripravci za modificiranje ciljnog lokusa
EP4089175A1 (en) * 2015-10-13 2022-11-16 Duke University Genome engineering with type i crispr systems in eukaryotic cells
US11311582B2 (en) * 2015-11-19 2022-04-26 Locus Biosciences, Inc. Bacteriophage compositions and methods of use thereof
US11542466B2 (en) * 2015-12-22 2023-01-03 North Carolina State University Methods and compositions for delivery of CRISPR based antimicrobials
WO2017223101A1 (en) * 2016-06-22 2017-12-28 The United States Of America As Represented By The Secretary Of The Navy Bacteriophage compositions and methods of selection of components against specific bacteria
US11672839B2 (en) * 2016-12-05 2023-06-13 Technechnophage, Investigacao E Desenvolvimento Em Biotecnologia, Sa Bacteriophage compositions comprising respiratory antibacterial phages and methods of use thereof
GB201710126D0 (en) * 2017-06-25 2017-08-09 Snipr Tech Ltd Vectors & Methods
KR20210006344A (ko) 2018-03-25 2021-01-18 에스엔아이피알 바이옴 에이피에스. 미생물 감염의 치료 및 예방

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017513489A (ja) * 2014-04-14 2017-06-01 ネメシス バイオサイエンス リミティド 治療薬
JP2017536831A (ja) * 2014-11-26 2017-12-14 テクノロジー イノベーション モメンタム ファンド（イスラエル）リミテッド パートナーシップＴｅｃｈｎｏｌｏｇｙ Ｉｎｎｏｖａｔｉｏｎ Ｍｏｍｅｎｔｕｍ Ｆｕｎｄ（ｉｓｒａｅｌ）Ｌｉｍｉｔｅｄ Ｐａｒｔｎｅｒｓｈｉｐ 細菌遺伝子の標的化削減
WO2016177682A1 (en) * 2015-05-06 2016-11-10 Snipr Technologies Limited Altering microbial populations & modifying microbiota
WO2016205276A1 (en) * 2015-06-15 2016-12-22 North Carolina State University Methods and compositions for efficient delivery of nucleic acids and rna-based antimicrobials

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EP3788152A4 (en) 2022-03-09
CN112424363A (zh) 2021-02-26
AU2019262200A1 (en) 2020-12-17
US20220002681A1 (en) 2022-01-06
US20220387531A1 (en) 2022-12-08
WO2019213592A1 (en) 2019-11-07
UY38215A (es) 2019-11-29
US20200354690A1 (en) 2020-11-12
US20230038106A1 (en) 2023-02-09
TW202014519A (zh) 2020-04-16
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