JP2021182878A - Bread dough and bread and method for producing the same - Google Patents

Bread dough and bread and method for producing the same Download PDF

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JP2021182878A
JP2021182878A JP2020089083A JP2020089083A JP2021182878A JP 2021182878 A JP2021182878 A JP 2021182878A JP 2020089083 A JP2020089083 A JP 2020089083A JP 2020089083 A JP2020089083 A JP 2020089083A JP 2021182878 A JP2021182878 A JP 2021182878A
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lactic acid
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潔丈 中道
Kiyotake Nakamichi
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Pando Co Ltd
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Abstract

To provide a method for producing a bread dough for producing bread with excellent flavor, texture, and a long storage period without using a subsidiary ingredient or a food additive.SOLUTION: A method for producing the bread dough includes a step in which a yeast base material containing lactic acid bacteria that includes a specified bacterial flora and water are mixed at a weight ratio of 1:1 to 1:9 to obtain a lactic acid bacterial strain. The lactic acid bacterial strain and vegetable oil are put in a pluggable container at a weight ratio of 9:1 to 1:1, the container is plugged, and a content is agitated, emulsified and made to stand still to obtain an emulsification strain. The emulsification strain and a medium derived from a natural product are put in the pluggable container at a weight ratio of 1:1 to 1:15 and mixed, the container is plugged, and the mixture is made to stand still to obtain a fermentation strain. The fermentation strain and flour are mixed at a weight ratio of 1:1 to 1:4, and 0.5 to 2 weight% of salt and 9 to 11 weight% of vegetable oil relative to the flour are added and mixed, and the mixture is kneaded by a mixer to produce gluten. The kneaded mixture is put in a fermentation container, the content is fermented, and a fermented product is made to stand still at 20 to 30°C for 1 to 8 hours for further fermentation.SELECTED DRAWING: None

Description

本発明は、パン生地及びパン類、並びにそれらの製造方法に関するものである。さらに詳しくは、本発明は、自然発酵システムにおいて形成された特定の菌叢を利用したパン生地及びパン類、並びにそれらの製造方法に関するものである。 The present invention relates to bread dough and breads, and a method for producing them. More specifically, the present invention relates to bread dough and breads utilizing a specific flora formed in a natural fermentation system, and a method for producing them.

酵母はパンを膨らませる上で、欠かせない原材料の一つであり、乳酸菌はパンの香りや食感といったパンの品質に良い影響を与えることが知られている。酵母と乳酸菌で構成されているパン種は、パンを膨らませる上で重要な役割を果たすと共に、風味づけや保存性向上、生地酸性化によるゲル化促進などの副次的な役割も担うことが知られている。酵母と乳酸菌で構成されているパン種は、ホップス種、パネトーネ種、ライサワー種、ルヴァン種、サンフランシスコサワー種などが古くから知られているが、これらのパン種は乳酸菌と酵母が共存するため、パンの膨らみを安定させるためには、管理に多大な労力や経験が必要とされてきた。 Yeast is one of the indispensable raw materials for bread swelling, and lactic acid bacteria are known to have a positive effect on bread quality such as bread aroma and texture. It is known that leaven composed of yeast and lactic acid bacteria plays an important role in expanding bread, and also plays a secondary role in flavoring, improving storage stability, and promoting gelation by acidifying the dough. Has been done. The leaven composed of yeast and lactic acid bacteria has long been known to be Hops, Panettone, Lysour, Levin, San Francisco sour, etc., but these leaven species coexist with lactic acid bacteria and yeast, so the leaven of bread In order to stabilize the bulge, a great deal of effort and experience has been required for management.

1600年以降にオランダのレーウェンフックが酵母の分離、培養に成功し、1857年にフランスのパスツールが酵母の発酵原理を解明した後、パン種の役割は徐々に工業的に製造された酵母である製パン用イースト(サッカロミセス・セルビシエ)に置き換えられ、製パンの工業化が進んだ。製パン用イーストは比較的安定してパンを膨らませることができるために、大規模ベーカリーの発展に大きく貢献しながら、利用が拡大していった。そして、現在ではほとんどのパンに製パン用イーストが使用されており、パンを膨らませるために欠かせない原材料の一つとなった。その一方で、本来乳酸菌が担っていたパンの香りや食感を出す役割は、砂糖、バター、卵、ショートニングなどの副材料、さらには多種多様な食品添加物に置き換えられてしまった。 After 1600, Lewenhook of the Netherlands succeeded in separating and culturing yeast, and Pasteur of France elucidated the fermentation principle of yeast in 1857, the role of leaven gradually became industrially produced yeast. It was replaced by a certain yeast for bread making (Saccharomyces cervisier), and the industrialization of bread making progressed. Since baker's yeast for bread making can inflate bread relatively stably, its use has expanded while greatly contributing to the development of large-scale bakeries. Nowadays, baker's yeast is used for most breads, and it has become one of the indispensable raw materials for inflating bread. On the other hand, the role of lactic acid bacteria to bring out the aroma and texture of bread has been replaced by auxiliary ingredients such as sugar, butter, eggs and shortening, as well as a wide variety of food additives.

砂糖、バター、卵、ショートニングなどの副材料は、主食のパンで日常的に摂取し続けるとカロリー過剰による生活習慣病を引き起こす原因と成り得る。 Secondary ingredients such as sugar, butter, eggs, and shortenings can cause lifestyle-related diseases due to excess calories if continued to be consumed on a daily basis in staple food bread.

また食品添加物は、ほとんどが人工的に作られた化学物質である。 Most food additives are artificially made chemicals.

イーストフードは、イースト菌発酵を促進するために加える添加物であり、イーストフードを使えば、発酵時間を短縮でき、安定的に大量生産することが可能となる。イーストフードは、塩化アンモニウム、塩化マグネシウム、炭酸カリウム(無水)、炭酸カルシウム、硫酸アンモニウム、硫酸カルシウム、硫酸マグネシウム、リン酸二水素カリウム、リン酸三カルシウム、焼成カルシウムなどから構成される。 Yeast food is an additive added to promote yeast fermentation, and if yeast food is used, the fermentation time can be shortened and stable mass production becomes possible. Yeast food is composed of ammonium chloride, magnesium chloride, potassium carbonate (anhydrous), calcium carbonate, ammonium sulfate, calcium sulfate, magnesium sulfate, potassium dihydrogen phosphate, tricalcium phosphate, calcined calcium and the like.

乳化剤は、互いに混ざりにくい水と油を混ぜるために加える添加物であり、グリセリン脂肪酸エステル、ソルビタン脂肪酸エステル、プロピレングリコール脂肪酸エステル、ショ糖脂肪酸エステルなどから構成される。 The emulsifier is an additive added to mix water and oil that are difficult to mix with each other, and is composed of glycerin fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester, sucrose fatty acid ester and the like.

膨張剤は、パン生地をスポンジ状の多孔質にし、膨らませるために加える添加物であり、炭酸水素ナトリウム(重曹)、硫酸アルミニウムカリウム(ミョウバン)などから構成される。 The leavening agent is an additive added to make the bread dough spongy and porous and to inflate it, and is composed of sodium hydrogen carbonate (baking soda), potassium aluminum sulfate (alum) and the like.

酸化防止剤は、酸化防止のために加える添加物であり、アスコルビン酸(ビタミンC)などである。 The antioxidant is an additive added for antioxidant, such as ascorbic acid (vitamin C).

香料は、香気を付与又は増強するために加えられる添加物であり、化学的に合成された合成香料と、天然物由来の天然香料がある。 Fragrances are additives added to impart or enhance aroma, and include chemically synthesized synthetic fragrances and natural fragrances derived from natural products.

主食のパンと共にこれらの食品添加物を日常的に摂取し続けると、人体に悪影響を及ぼすおそれがある。 Continued daily intake of these food additives along with staple food bread may have adverse effects on the human body.

近年、製パンにおいて前記のような副材料や食品添加物の使用を減らす試みとして、原材料を乳酸菌で発酵させた「発酵種」などが広く使われるようになっている In recent years, as an attempt to reduce the use of auxiliary materials and food additives as described above in bread making, "fermented seeds" obtained by fermenting raw materials with lactic acid bacteria have become widely used.

また、特開2015−204810号公報は、自然発酵システムを利用して酵母と乳酸菌とを共生させた菌叢を含む食品用基材を開示している。 Further, Japanese Patent Application Laid-Open No. 2015-204810 discloses a food base material containing a flora in which yeast and lactic acid bacteria coexist using a natural fermentation system.

特開2015−204810号公報Japanese Unexamined Patent Publication No. 2015-204810

副材料や食品添加物を使用せずに、高い風味、食感を持ち、保存期間の長いパン類を製造することが求められている。 It is required to produce breads having a high flavor and texture and a long shelf life without using auxiliary materials or food additives.

本発明者らは、前記課題を解決すべく鋭意検討を行なった結果、特開2015−204810号公報に記載された自然発酵システムにおいて製造された乳酸菌含有酵母基材に着目し、各種発酵条件を最適化することにより、バターや砂糖、卵、ショートニングなどの副材料、又はイーストフード、香料などの食品添加物を一切使用していないのにもかかわらず、風味豊かなパン類を製造することに成功し、本発明の完成に至った。 As a result of diligent studies to solve the above problems, the present inventors have focused on the lactic acid bacteria-containing yeast substrate produced in the natural fermentation system described in JP-A-2015-204810, and set various fermentation conditions. By optimizing, we decided to produce flavorful breads without using any secondary ingredients such as butter, sugar, eggs, shortenings, or food additives such as yeast foods and flavors. It was successful and led to the completion of the present invention.

以下、本発明の実施形態の例について詳細に説明する。以下の本実施形態は、本発明を説明するための例示であり、本発明を以下の内容に限定する趣旨ではない。 Hereinafter, examples of embodiments of the present invention will be described in detail. The following embodiments are examples for explaining the present invention, and are not intended to limit the present invention to the following contents.

本発明は、以下の技術的手段から構成される。 The present invention comprises the following technical means.

(1)自然発酵システムを利用したパン生地の製造方法であって、
(A)特定の菌叢を含む乳酸菌含有酵母基材、及び水を1:1〜1:9の重量比で混合して、5〜30℃で1〜10日間静置して、乳酸菌種を得る工程、
(B)施栓可能な容器に、前記乳酸菌種、及び植物性油脂を9:1〜1:1の重量比で入れて施栓し、撹拌し乳化させた後、5〜25℃で、20〜30時間静置することにより乳化種を得る工程、
(C)施栓可能な容器に、前記乳化種、及び天然物由来培地を1:1〜1:15の重量比で混合した後、施栓し5〜25℃で20〜30時間静置することにより発酵種を得る工程、
(D)前記発酵種、及び小麦粉を1:1〜1:4重量比で混合し、さらに前記小麦粉に対し、0.5〜2重量%の塩、及び9〜11重量%の植物性油脂を加えて混合する工程、
(E)室温を16〜25℃、前記混合物の内部温度を26〜30℃に保ち、前記小麦粉に対し、9〜11重量%の植物性油脂を添加しながら、ミキサーで前記混合物を低速(50回/分〜200回/分の回転率)で、5秒〜20分間、中速(80回/分〜400回/分の回転率)で5秒〜20分間、その後、高速(100回/分〜500回/分の回転率)で、5秒〜20分間捏ね上げてグルテンを出す工程、
(F)前記捏ね上げた混合物を発酵容器に入れ、2〜8℃の低温下で、24〜50時間静置し、内容物を発酵させる工程(0次発酵)、及び
(G)前記0次発酵物を、20〜30℃で1〜8時間静置し、さらに発酵させる工程(1次発酵)、を含むパン生地の製造方法。
(1) A method for producing bread dough using a natural fermentation system.
(A) A yeast substrate containing lactic acid bacteria containing a specific flora and water are mixed at a weight ratio of 1: 1 to 1: 9 and allowed to stand at 5 to 30 ° C. for 1 to 10 days to obtain lactic acid bacteria species. Getting process,
(B) The lactic acid bacterium species and vegetable oils and fats are placed in a cappable container at a weight ratio of 9: 1 to 1: 1, capped, stirred and emulsified, and then 20 to 30 at 5 to 25 ° C. The process of obtaining an emulsified seed by allowing it to stand for a while,
(C) The emulsified seed and the natural product-derived medium are mixed in a cappable container at a weight ratio of 1: 1 to 1:15, then plugged and allowed to stand at 5 to 25 ° C. for 20 to 30 hours. The process of obtaining fermented seeds,
(D) The fermented seeds and wheat flour are mixed in a ratio of 1: 1 to 1: 4, and 0.5 to 2% by weight of salt and 9 to 11% by weight of vegetable fat and oil are further added to the flour. In addition, the mixing process,
(E) Keep the room temperature at 16 to 25 ° C. and the internal temperature of the mixture at 26 to 30 ° C., add 9 to 11% by weight of vegetable oil to the flour, and slow down (50) the mixture with a mixer. Rotation rate of times / minute to 200 times / minute) for 5 seconds to 20 minutes, medium speed (rotation rate of 80 times / minute to 400 times / minute) for 5 seconds to 20 minutes, and then high speed (100 times / minute / minute). A process of kneading for 5 to 20 minutes to produce gluten at a rotation rate of 1 to 500 times / minute.
(F) The step (0th-order fermentation) in which the kneaded mixture is placed in a fermentation vessel and allowed to stand at a low temperature of 2 to 8 ° C. for 24 to 50 hours to ferment the contents, and (G) the 0th-order A method for producing bread dough, which comprises a step of allowing the fermented product to stand at 20 to 30 ° C. for 1 to 8 hours and further fermenting it (primary fermentation).

(2)前記乳酸菌含有酵母基材が、乳酸球菌と酵母の共生状態がみられ、かつ、乳酸球菌が優勢の状態を保ち、表面に「つや膜」が形成され、非加熱で雑菌が不検出(1万個以下/g)の菌叢を含む、(1)に記載のパン生地の製造方法。 (2) The lactic acid bacterium-containing yeast substrate shows a symbiotic state of lactic acid bacterium and yeast, and the lactic acid bacterium remains predominant, a "glossy film" is formed on the surface, and no germs are detected without heating. The method for producing bread dough according to (1), which comprises a bacterial flora of (10,000 or less / g).

(3)前記小麦粉が、9〜11.5%のタンパク質を含有する、(1)又は(2)に記載のパン生地の製造方法。 (3) The method for producing a bread dough according to (1) or (2), wherein the wheat flour contains 9 to 11.5% protein.

(4)前記天然物由来培地が、穀物由来物、植物由来物、又は乳由来物を含む、(1)〜(3)のいずれかに記載のパン生地の製造方法。 (4) The method for producing a bread dough according to any one of (1) to (3), wherein the natural product-derived medium contains a grain-derived product, a plant-derived product, or a milk-derived product.

(5)自然発酵システムを利用したパン類の製造方法であって
(H)(1)〜(4)のいずれかに記載の製造方法により得られたパン生地を型に入れ成形する工程、
(I)前記成形したパン生地を28〜30℃に保ち、0.75〜3時間静置し発酵させる工程(2次発酵)、及び
(J)前記2次発酵物を、上火160〜230℃、下火210〜270℃のオーブンで、15〜40分間焼成する工程を含む、パン類の製造方法。
(5) A method for producing bread using a natural fermentation system, wherein the bread dough obtained by the production method according to any one of (H) (1) to (4) is placed in a mold and molded.
(I) The step of keeping the molded bread dough at 28 to 30 ° C. and allowing it to stand for 0.75 to 3 hours to ferment (secondary fermentation), and (J) the secondary fermented product is heated to 160 to 230 ° C. A method for producing bread, which comprises a step of baking for 15 to 40 minutes in an oven at a lower heat of 210 to 270 ° C.

(6)(1)〜(4)のいずれかに記載の方法により製造されたパン生地。 (6) Bread dough produced by the method according to any one of (1) to (4).

(7)(5)に記載の方法により製造されたパン類。 (7) Breads produced by the method according to (5).

次に、本発明についてさらに詳細に説明する。 Next, the present invention will be described in more detail.

工程(A)において用いられる乳酸菌含有酵母基材は、以下の自然発酵システム(i)〜(v)の工程のうち、工程(ii)〜(iv)において得られた菌叢(ii)〜(iv)から選定される菌叢を含むものであり、以下の工程に従って製造することができる。また該乳酸菌含有酵母基材は、商業的に入手することもできる(ウエダ家、自然発酵乳酸菌)。 The lactic acid bacterium-containing yeast substrate used in the step (A) is the bacterial flora (ii) to (ii) obtained in the steps (ii) to (iv) among the following steps of the natural fermentation system (i) to (v). It contains a bacterial flora selected from iv) and can be produced according to the following steps. The yeast base material containing lactic acid bacteria can also be obtained commercially (Ueda family, naturally fermented lactic acid bacteria).

工程(i)
施栓可能な容器に原料の果実及び/又は野菜を入れ、施栓後、これを、容器詰めの状態の準密閉系で、2℃〜6℃の低温下の保存工程で保存し、これを15℃〜30℃の常温下の保存工程で保存して、原料に付着している自生の微生物と原料由来糖分を利用して自然発酵させる自然発酵システムを利用して膜状の表面形態を呈する特定の菌叢を形成させ、バクテリアと乳酸菌の生息がみられる菌叢(i)を形成させる。
Step (i)
Put the raw material fruits and / or vegetables in a cappable container, and after plugging, store this in a semi-sealed system in a container-packed state in a storage step at a low temperature of 2 ° C to 6 ° C, and store this at 15 ° C. Specific that exhibits a film-like surface morphology using a natural fermentation system that is stored in a storage process at room temperature of ~ 30 ° C and is naturally fermented using native microorganisms adhering to the raw material and sugar derived from the raw material. It forms a flora and forms a flora (i) in which bacteria and lactic acid bacteria inhabit.

工程(ii)
菌叢(i)を、さらに15℃〜30℃の常温下に保存して、菌叢(i)に比べて、バクテリアが存在せず、乳酸菌が増えて、乳酸球菌と酵母の共生状態がみられ、かつ、乳酸球菌が優勢の状態を保ち、表面に「つや膜」が形成され、非加熱で雑菌が不検出(1万個以下/g)の菌叢(ii)を形成させる。
Process (ii)
The bacterial flora (i) was further stored at room temperature of 15 ° C to 30 ° C, and compared to the bacterial flora (i), bacteria did not exist, lactic acid bacteria increased, and a symbiotic state of lactic acid bacteria and yeast was observed. In addition, lactic acid bacteria remain predominant, a "glossy film" is formed on the surface, and bacteria are not detected (10,000 or less / g) without heating to form a flora (ii).

工程(iii)
菌叢(ii)を、さらに15℃〜30℃の常温下に保存して、バクテリアが存在せず、菌叢(ii)に比べて、乳酸菌と、酵母がより増えて、乳酸球菌と酵母の共生状態がみられ、かつ、乳酸球菌が優勢の状態を保ち、表面に「つや膜」が形成され、非加熱で雑菌が不検出(1万個以下/g)の菌叢(iii)を形成させる。
Process (iii)
The bacterial flora (ii) was further stored at room temperature of 15 ° C to 30 ° C, and bacteria were absent. A symbiotic state is observed, and lactic acid bacteria remain predominant, a "glossy film" is formed on the surface, and bacteria are not detected (10,000 or less / g) without heating to form a flora (iii). Let me.

工程(iv)
施栓可能な容器に原料の乳酸菌を含む水、蒸し又は炊いた穀物と麹、水を入れ、施栓後、これを、容器詰めの状態の準密閉系で、2℃〜6℃の低温下の保存工程で保存して、原料に付着している自生の微生物と原料由来糖分を利用して自然発酵させる自然発酵システムを利用して膜状の表面形態を呈する特定の菌叢を形成させ、バクテリアが存在せず、乳酸球菌と酵母の共生状態がみられ、かつ、乳酸球菌が優勢の状態を保ち、表面に「つや膜」が形成され、非加熱で雑菌が不検出(1万個以下/g)の菌叢(iv)を形成させる。
Process (iv)
Put water containing lactic acid bacteria as a raw material, steamed or cooked grains and koji, and water in a container that can be plugged, and after plugging, store this in a semi-sealed system packed in a container at a low temperature of 2 ° C to 6 ° C. Bacteria form a specific flora that exhibits a film-like surface morphology using a natural fermentation system that is preserved in the process and naturally fermented using native microorganisms adhering to the raw material and sugar derived from the raw material. It does not exist, a symbiotic state of lactic acid bacteria and yeast is observed, and the lactic acid bacteria remain predominant, a "glossy film" is formed on the surface, and no germs are detected without heating (10,000 or less / g). ) Is formed.

行程(v)
前記工程からなる容器詰めの状態の準密閉系での自然発酵システムにおいて、特に、前記工程(ii)から(iv)のいずれかの工程で得られる菌叢を拡張し、拡大(スケールアップ)して、該菌叢を含むパン類の生産に適用できる乳酸菌含有酵母基材とする。
Itinerary (v)
In a natural fermentation system in a semi-enclosed system in a packaged state consisting of the above steps, in particular, the bacterial flora obtained in any of the steps (ii) to (iv) is expanded and expanded (scaled up). Therefore, a yeast base material containing lactic acid bacteria that can be applied to the production of breads containing the flora is used.

自然発酵システムによって形成される菌叢と該菌叢を構成する乳酸菌と酵母の質は、原料に付着している自生の微生物(乳酸菌と酵母)と原料由来糖分を利用して、制御された所定の低温条件下に所定の期間保存する低温保存工程を含む保存工程で形成された特定の菌叢と該菌叢を構成する特定の乳酸菌と酵母であることで特徴付けられる。 The bacterial flora formed by the natural fermentation system and the quality of the lactic acid bacteria and yeast that compose the bacterial flora are controlled by utilizing the native microorganisms (lactic acid bacteria and yeast) adhering to the raw material and the sugar content derived from the raw material. It is characterized by a specific flora formed in a storage step including a low temperature storage step of storing under low temperature conditions of the above, and specific lactic acid bacteria and yeast constituting the flora.

乳酸菌とは、植物性乳酸菌であって、連鎖状の球菌のことである。乳酸菌が他の微生物、特に、病原菌や腐敗菌の生育を、乳酸菌が生成する抗菌性物質によって阻止する作用(バイオプリサバティブ)はよく知られているが、前記菌叢に含まれる乳酸菌は、有害細菌に制菌効果を発揮する乳酸菌であるラクトコッカス属、ロイコノストック属などに属する球菌であり、球状の形状を呈する形態上の特徴がある。 Lactic acid bacteria are plant-derived lactic acid bacteria and are chained cocci. It is well known that lactic acid bacteria block the growth of other microorganisms, especially pathogenic bacteria and spoilage bacteria, by antibacterial substances produced by lactic acid bacteria (biopreservative). It is a coccus belonging to the genus Lactococcus, Leuconostocaceae, etc., which are lactic acid bacteria that exert an antibacterial effect on harmful bacteria, and has a morphological characteristic of having a spherical shape.

また、米などの穀物を原料とする自然発酵については、米などの糖化に使用する米麹、玄米麹などは、他原料による自然発酵液に比べると、タンパク質含有量を分解する能力が高い、ラクトコッカス属などの乳酸菌を多く含む。 Regarding natural fermentation using grains such as rice as raw materials, rice jiuqu and brown rice jiuqu used for saccharification of rice etc. have a higher ability to decompose protein content than natural fermented liquids made from other raw materials. It contains a lot of lactic acid bacteria such as lactococcus.

酵母とは、サッカロミセス属などの多極出芽酵母であり、前記菌叢では、酵母に比べて乳酸菌(連鎖球菌)が優勢な状態にあるが、酵母の至適生育下(20〜25℃の好気的条件)に置かれると、2〜3日後に10の8乗(億単位)の増殖をする。これは、たとえば、製パンにおいては、製パン用イーストを補糖する条件で該製パン用イーストが増殖する期間と同等の発酵力といえるものであり、また、自家製酵母の増殖に必要とされる粉や糖類も必要ない。 Yeast is a multipolar budding yeast such as Saccharomyces genus, and lactic acid bacteria (streptococci) are predominant in the flora in the flora, but the yeast is under optimum growth (20 to 25 ° C.). When placed in the temperate condition), it proliferates to the 10th power (100 million units) after 2 to 3 days. For example, in bread making, it can be said that the fermenting power is equivalent to the period during which the baker's yeast for bread making grows under the condition of supplementing the yeast for bread making, and it is also required for the growth of homemade yeast. No need for yeast or sugar.

前記菌叢を含む自然発酵液については、加熱殺菌処理を施さずとも大腸菌や大腸菌群などの雑菌は不検出であり、大腸菌:陰性/g、大腸菌群:陰性/g、pH4.0前後として表わされる。ここで、pH4.0前後とは、食中毒菌などの有害な細菌が生育できない弱酸性を意味する。 In the natural fermented liquor containing the bacterial flora, bacteria such as Escherichia coli and coliform bacteria were not detected even without heat sterilization treatment, and were expressed as Escherichia coli: negative / g, coliform bacteria: negative / g, pH around 4.0. Is done. Here, around pH 4.0 means weak acidity in which harmful bacteria such as food poisoning bacteria cannot grow.

「つや膜」とは、細胞多糖(EPS)により表面に形成された、目視的に「つや」(艶)を呈した膜状の形態を言い、「つや膜ネットワーク」が生成されることを意味する。 "Glossy membrane" refers to a film-like morphology that is visually "glossy" (glossy) formed on the surface by cellular polysaccharides (EPS), and means that a "glossy membrane network" is generated. do.

細胞外多糖(EPS)とは、多くの微生物に共通して見られる細胞外構成物であり、微生物とそれ自身を取り巻く環境との間の相互作用や安定性にかかわる重要な因子の一つと考えられる。 Extracellular polysaccharides (EPS) are extracellular constituents commonly found in many microorganisms and are considered to be one of the important factors involved in the interaction and stability between microorganisms and the environment surrounding them. Be done.

「つや膜ネットワーク」とは、野生の「乳酸菌と酵母」の共生関係を保つ細胞外多糖(EPS)によるものである。 The "glossy membrane network" is due to the extracellular polysaccharide (EPS) that maintains a symbiotic relationship between wild "lactic acid bacteria and yeast".

本発明における乳酸菌含有酵母基材は、生きている「乳酸菌と酵母」の菌体と、該菌体の産生物質である酵素、アミノ酸、ミネラル、ビタミンなどを内包し、微生物間の相互作用にかかわる成熟したつや膜ネットワークが食品発酵基材の表面及び内部に形成されて、該つや膜ネットワークが食品発酵基材の表面及び内部を組成的及びマトリックス的に安定に保持する機能を果たしている。 The lactic acid bacterium-containing yeast substrate in the present invention contains live "lactic acid bacteria and yeast" cells and enzymes, amino acids, minerals, vitamins, etc. that are the substances produced by the cells, and is involved in the interaction between microorganisms. A mature glossy membrane network is formed on the surface and inside of the fermented food substrate, and the glossy membrane network functions to stably hold the surface and inside of the fermented food substrate in a compositionally and matrix-like manner.

本発明における乳酸菌含有酵母基材は、目的とするパン類の製造に応じて例えば以下のように使い分けることができる。以後、菌叢を、「COBO」又は「COBO液」と略記することがある。また、以後、本発明の方法において製造されたパン類を「YAWARA」と略記することがある。 The yeast base material containing lactic acid bacteria in the present invention can be used properly, for example, as follows, depending on the production of the desired bread. Hereinafter, the bacterial flora may be abbreviated as "COBO" or "COBO solution". Further, hereinafter, the breads produced by the method of the present invention may be abbreviated as "YAWARA".

・ベーグル製品
菌叢(ii)又は(iv)を含む乳酸菌含有酵母基材(COBO液)を利用することで、植物性乳酸菌が産生する多糖類によって、砂糖など添加せず、生地を作ることができる。
・ Bagel products By using a lactic acid bacterium-containing yeast base material (COBO solution) containing a bacterial flora (ii) or (iv), it is possible to make dough with polysaccharides produced by plant-derived lactic acid bacteria without adding sugar or the like. can.

・パン製品
菌叢(iii)又は(iv)を含む乳酸菌含有酵母基材(COBO液)を利用することで、COBO中に含まれる野生酵母の働きによって産生する炭酸ガスを利用して、工業用イーストや市販の天然酵母、糖分を添加せず、ふっくらとしたパンとなる。また、COBO中に含まれる乳酸菌の働きによって、生地のきめが細かくなり、しっとりやわらかな質感が得られ、消化にかかる負担を軽減するパン製品となる。完全無添加のパンは、10℃以下で、約1カ月の保存性をもつ。
-Bread products By using a lactic acid bacterium-containing yeast base material (COBO solution) containing a bacterial flora (iii) or (iv), carbon dioxide gas produced by the action of wild yeast contained in COBO is used for industrial use. It becomes a plump bread without adding yeast, commercially available natural yeast, or sugar. In addition, the action of lactic acid bacteria contained in COBO makes the dough finer, gives a moist and soft texture, and makes a bread product that reduces the burden on digestion. Completely additive-free bread has a shelf life of about 1 month at 10 ° C or lower.

・スコーン、ピザ
菌叢(iii)又は(iv)を含む乳酸菌含有酵母基材(COBO液)を利用することで、COBO中に含まれる野生酵母の働きによって産生する炭酸ガスを利用して、工業用イーストや市販の天然酵母、糖分を添加せず、生地のふくらみを有する製品(スコーン、ピザ) が得られる。また、COBO 中に含まれる乳酸菌の働きによって、生地のきめが細かくなり、しっとりやわらかな質感が得られ、消化にかかる負担を軽減し、COBO中に含まれるつや膜による乳化作用により、植物性油脂(紅花油、菜種油、オリーブオイルなど)を含む生地の消化にかかる負担を軽減する製品となる。
-By using a lactic acid bacterium-containing yeast substrate (COBO solution) containing scone, pizza flora (iii) or (iv), the carbon dioxide gas produced by the action of wild yeast contained in COBO is used for industry. Products (scocorn, pizza) with swelling of the dough can be obtained without adding yeast for use, commercially available natural yeast, or sugar. In addition, the action of lactic acid bacteria contained in COBO makes the texture of the dough finer, obtains a moist and soft texture, reduces the burden on digestion, and the emulsifying action of the glossy film contained in COBO makes vegetable oils and fats. It is a product that reduces the burden on digestion of dough containing (safflower oil, rapeseed oil, olive oil, etc.).

工程(A)では、特定の菌叢を含む乳酸菌含有酵母基材、及び水を1:1〜1:9の重量比、好ましくは1:1〜1:3の重量比で混合し、5〜30℃、好ましくは20〜25℃で、例えば、20時間〜10日間、好ましくは1〜3日間、さらに好ましくは20〜30時間静置することにより乳酸菌種を得る。 In step (A), a lactic acid bacterium-containing yeast substrate containing a specific flora and water are mixed at a weight ratio of 1: 1 to 1: 9, preferably 1: 1 to 1: 3, and 5 to 5 are mixed. Lactic acid bacteria species are obtained by allowing to stand at 30 ° C., preferably 20 to 25 ° C., for example, for 20 hours to 10 days, preferably 1 to 3 days, and more preferably 20 to 30 hours.

ここでの乳酸菌種とは、自然発酵において乳酸球菌と酵母が共生状態を作る菌叢が形成される発酵状態にあるものを言う。 The lactic acid bacterium species here refers to those in a fermented state in which a flora in which lactic acid bacteria and yeast form a symbiotic state is formed in natural fermentation.

工程(B)では、施栓可能な容器に、前記乳酸菌種、及び植物性油脂を9:1〜1:1の重量比、好ましくは3:1〜1:1の重量比で入れて施栓し、撹拌し乳化させた後、5〜25℃、好ましくは、5〜10℃の冷温下で、20〜30時間、好ましくは約24時間静置することにより乳化種を得る。 In the step (B), the lactic acid bacterium species and the vegetable oil / fat are placed in a cappable container at a weight ratio of 9: 1 to 1: 1, preferably 3: 1 to 1: 1 and plugged. After stirring and emulsifying, the emulsified seeds are obtained by allowing to stand at a cold temperature of 5 to 25 ° C., preferably 5 to 10 ° C. for 20 to 30 hours, preferably about 24 hours.

植物性油脂は、紅花油、菜種油、オリーブオイルなどの植物性油が用いられ、好ましくは、紅花油である。 As the vegetable oil, vegetable oil such as safflower oil, rapeseed oil, and olive oil is used, and safflower oil is preferable.

工程(C)では、施栓可能な容器に、前記乳化種、及び天然物由来培地を、1:1〜1:15の重量比、好ましくは1:1〜1:5の重量比で混ぜて撹拌した後、施栓し5〜25℃、好ましくは5〜8℃の冷温下で、20〜30時間、好ましくは約24時間静置することにより発酵種を得る。 In step (C), the emulsified seed and the natural product-derived medium are mixed and stirred in a cappable container at a weight ratio of 1: 1 to 1:15, preferably 1: 1 to 1: 5. After that, the fermented seeds are obtained by plugging and allowing to stand at a cold temperature of 5 to 25 ° C., preferably 5 to 8 ° C. for 20 to 30 hours, preferably about 24 hours.

ここでの天然物由来培地とは、乳化種の自然発酵を促すために添加される穀物由来、植物由来、又は乳由来の培地である。 The natural product-derived medium here is a grain-derived, plant-derived, or milk-derived medium added to promote the natural fermentation of an emulsified species.

穀物由来の培地を使用した場合、焼き上がったパン類は小麦の旨味と香り食感が鮮明に表現される。植物由来の培地を使用した場合、焼き上がったパン類は果実や野菜の味と香りで高品質の果実・野菜パンとなる。乳由来の培地を使用した場合、焼き上がったパン類はきれいな焼き色になり、風味が増す。これらの培地は、単独でも組み合わせても使用することができる。 When a grain-derived medium is used, the baked breads clearly express the umami and aroma texture of wheat. When a plant-derived medium is used, the baked bread becomes a high-quality fruit / vegetable bread with the taste and aroma of fruits and vegetables. When a milk-derived medium is used, the baked breads have a beautiful brown color and an increased flavor. These media can be used alone or in combination.

穀物由来の培地とは、原料に玄米、精米、もち米などの穀物を含む培地であり、糖分(デンプンなど)やタンパク質を含むものである。原料となる穀物は、玄米の場合、タンパク質とデンプンを主成分とし、精米、もち米の場合、デンプンを主成分とする。原料となる穀物は、従来の慣行栽培作物に比較して、農薬の使用がない、又は使用量が少ないもの、(化学)肥料の使用がない、又は完熟した有機肥料を適宜使用したものが望ましい。原料となる穀物の貯蔵条件は、貯蔵温度が年間を通して一定であることが望ましい。穀物由来の培地は、好ましくは粥状のモチ米を麹と共に50℃〜60℃の温度設定により、5時間〜10時間で発酵させた甘酒などである。 The grain-derived medium is a medium containing grains such as brown rice, polished rice, and glutinous rice as raw materials, and contains sugar (starch, etc.) and protein. In the case of brown rice, the main components of the grain are protein and starch, and in the case of polished rice and glutinous rice, the main components are starch. It is desirable that the grains used as raw materials are those that do not use or use less pesticides, do not use (chemical) fertilizers, or use ripe organic fertilizers as appropriate compared to conventional conventional crops. .. It is desirable that the storage temperature of the raw material grain is constant throughout the year. The grain-derived medium is preferably amazake, which is obtained by fermenting porridge-like glutinous rice together with jiuqu at a temperature of 50 ° C to 60 ° C for 5 to 10 hours.

植物由来の培地とは、原料に果実や野菜を含む培地であり、糖分(ショ糖、ブドウ糖、果糖など)や有機酸(リンゴ酸、クエン酸、酒石酸など)を含むものである。原料となる果実又は野菜は、乳酸菌や酵母が好む品種であり、かつ、熟し過ぎておらず、糖分や有機酸が消費尽くされていないもの、が望ましい。原料となる果実又は野菜は、従来の慣行栽培作物に比較して、農薬の使用がない、又はその使用量が少ないもの、(化学)肥料の使用がない、又は完熟した有機肥料を適宜使用したもの、が望ましい。原料となる果実又は野菜の貯蔵温度は、年間を通して一定であることが望ましい。植物由来の培地は、好ましくはリンゴ果汁、ブドウ果汁などである。 The plant-derived medium is a medium containing fruits and vegetables as a raw material, and contains sugar (sucrose, glucose, fructose, etc.) and organic acids (malic acid, citric acid, tartaric acid, etc.). It is desirable that the fruits or vegetables used as raw materials are varieties preferred by lactic acid bacteria and yeast, are not overripe, and are not completely consumed in sugar and organic acids. As the raw material fruits or vegetables, those that do not use or use less pesticides, do not use (chemical) fertilizers, or use ripe organic fertilizers as appropriate compared to conventional conventional crops. Things are desirable. It is desirable that the storage temperature of the raw material fruit or vegetable is constant throughout the year. The medium derived from the plant is preferably apple juice, grape juice and the like.

乳由来の培地とは、原料にウシやヤギなどの乳を含む培地であり、糖分(乳糖など)やタンパク質(ホエイ、カゼインなど)を含むものである。乳由来の培地は、好ましくは新鮮な牛乳やヤギミルクであり、特にヤギミルクは牛乳に比べて脂肪球の比率が小さく消化しやすい点でより好ましい。 The milk-derived medium is a medium containing milk such as cow or goat as a raw material, and contains sugar (lactose or the like) or protein (whey, casein or the like). The milk-derived medium is preferably fresh milk or goat milk, and in particular, goat milk is more preferable because it has a smaller proportion of fat globules than milk and is easy to digest.

工程(D)では、前記発酵種、及びタンパク質含有量9〜11.5%、好ましくは10〜11.5%の小麦粉を、1:1〜1:4の重量比、好ましくは1:1〜1:3の重量比で混合し、さらに前記小麦粉に対し、塩を0.5〜2重量%、好ましくは約1重量%、及び植物性油脂を9〜11重量%、好ましくは約10重量%の量で加えて混合する。 In step (D), the fermented species and wheat flour having a protein content of 9 to 11.5%, preferably 10 to 11.5%, were added in a weight ratio of 1: 1 to 1: 4, preferably 1: 1 to 1. Mix at a weight ratio of 1: 3, and add 0.5 to 2% by weight, preferably about 1% by weight, and 9 to 11% by weight of vegetable fat, preferably about 10% by weight, based on the flour. Add in the amount of and mix.

ここでの小麦粉とは、パン類の主原料である硬質小麦粉である。硬質小麦粉は、粒が固くタンパク質が多い小麦粉であり、タンパク質含有量11.5〜12.0%の強力粉と、タンパク質含有量9〜11.5%の準強力粉に分けられる。 The wheat flour here is hard wheat flour, which is the main raw material for breads. Hard wheat flour is a wheat flour having a hard grain and a large amount of protein, and is divided into a strong flour having a protein content of 11.5 to 12.0% and a semi-strong flour having a protein content of 9 to 11.5%.

一般の製パンでは、タンパク質含有量が11.5〜12.0%の強力粉を用いるが、本発明にかかるパン類の製造では、タンパク質含有量がより少ない準強力粉を用いるため、柔らかく、なめらかな食感に仕上がる。 In general bread making, strong flour having a protein content of 11.5 to 12.0% is used, but in the bread making according to the present invention, semi-strong flour having a lower protein content is used, so that it is soft and smooth. Finished in texture.

好ましくは、小麦粉は、タンパク質含有量が11.3%の「はるゆたか」、タンパク質含有量が11.0%の「ゆめかおり」などの品種改良の少ない国産の品種である。 Preferably, the wheat flour is a domestically produced variety such as "Haruyutaka" having a protein content of 11.3% and "Yumekaori" having a protein content of 11.0%.

ここでの塩は、パン類の味の決め手となり、生地のガス保持力を高めるために用いられる。好ましくはミネラル自然海塩である。 The salt here is the decisive factor for the taste of bread and is used to enhance the gas retention of the dough. It is preferably a mineral natural sea salt.

ここでの植物性油脂は、紅花油、菜種油、オリーブオイルなどの植物性油であり、好ましくは、紅花油である。 The vegetable oil and fat here is a vegetable oil such as safflower oil, rapeseed oil, and olive oil, and is preferably safflower oil.

工程(E)では、室温を16〜25℃、好ましくは約25℃、前記混合物の内部温度を26〜30℃、好ましくは約28℃に保ち、前記小麦粉に対し、9〜11重量%、好ましくは約10重量%の植物性油脂を添加しながら、ミキサーで前記混合物を低速(50回/分〜200回/分の回転率、好ましくは110回/分〜130回/分の回転率)、中速(80回/分〜400回/分の回転率、好ましくは220回/分〜260回/分の回転率)、高速(100回/分〜500回/分の回転率、好ましくは320回/分〜400回/分の回転率)で、それぞれ5秒〜20分間、好ましくはそれぞれ50秒〜10分間捏ね上げてグルテンを出す。 In step (E), the room temperature is maintained at 16 to 25 ° C., preferably about 25 ° C., and the internal temperature of the mixture is maintained at 26 to 30 ° C., preferably about 28 ° C., 9 to 11% by weight, preferably 9 to 11% by weight based on the flour. While adding about 10% by weight of vegetable oil, the mixture was slowly mixed with a mixer (rotation rate of 50 times / minute to 200 times / minute, preferably 110 times / minute to 130 times / minute). Medium speed (80 times / minute to 400 times / minute rotation rate, preferably 220 times / minute to 260 times / minute rotation rate), high speed (100 times / minute to 500 times / minute rotation rate, preferably 320 times / minute) Knead for 5 seconds to 20 minutes, preferably 50 seconds to 10 minutes, respectively, at a rotation rate of times / minute to 400 times / minute to release gluten.

ここでの植物性油脂は、紅花油、菜種油、オリーブオイルなどの植物性油であり、好ましくは、紅花油である。 The vegetable oil and fat here is a vegetable oil such as safflower oil, rapeseed oil, and olive oil, and is preferably safflower oil.

工程(F)では、前記捏ね上げた混合物を発酵容器に入れ、2〜8℃、好ましくは2〜4℃の低温下で、24〜50時間、好ましくは24〜36時間静置し、内容物を発酵させる(0次発酵)。 In the step (F), the kneaded mixture is placed in a fermentation vessel and allowed to stand at a low temperature of 2 to 8 ° C, preferably 2 to 4 ° C for 24 to 50 hours, preferably 24 to 36 hours, and the contents. Is fermented (0th-order fermentation).

工程(G)では、前記0次発酵物を、20〜30℃で、好ましくは25〜30℃で、1〜8時間、好ましくは1〜2時間静置し、さらに発酵させて、パン生地を完成させる(1次発酵)。 In the step (G), the 0th-order fermented product is allowed to stand at 20 to 30 ° C., preferably 25 to 30 ° C. for 1 to 8 hours, preferably 1 to 2 hours, and further fermented to complete the bread dough. Let (primary fermentation).

工程(H)では、前記パン生地を型に入れて成形する。型は、種類を問わず使用できる。 In the step (H), the bread dough is placed in a mold and molded. The type can be used regardless of the type.

工程(I)では、前記成形したパン生地を28〜30℃、好ましくは約29℃に保ち、0.75〜3時間、好ましくは1〜2時間静置し発酵させる(2次発酵)。 In the step (I), the molded bread dough is kept at 28 to 30 ° C., preferably about 29 ° C., and allowed to stand for 0.75 to 3 hours, preferably 1 to 2 hours to ferment (secondary fermentation).

工程(J)では、前記2次発酵物を上火160〜230℃、好ましくは180〜210℃、下火210〜270℃、好ましくは230〜250℃のオーブンで15〜40分間、好ましくは25〜30分間焼成してパン類を製造する。 In step (J), the secondary fermented product is placed in an oven at a top heat of 160 to 230 ° C., preferably 180 to 210 ° C., a bottom heat of 210 to 270 ° C., preferably 230 to 250 ° C. for 15 to 40 minutes, preferably 25. Bread is produced by baking for ~ 30 minutes.

前記工程(A)〜(J)から製造されるパン類とは、硬質小麦粉を主原料としたパン、ベーグル、饅頭、ピザ、ナンなどを言い、世界各地でその地域の独自の食文化の中で多種多様な形態で存在している食品を言う。 Breads produced from the above steps (A) to (J) refer to breads, bagels, buns, pizzas, naan, etc. made from hard wheat flour, and are included in the unique food culture of each region around the world. Refers to foods that exist in a wide variety of forms.

また、前記工程(A)〜(J)から製造されるパン類は、バター、砂糖、卵などの副材料、または多種多様な具材、スパイスなどを使用することにより、多種多様な菓子パンや総菜パンに加工することができる。 In addition, the breads produced from the steps (A) to (J) use a wide variety of sweet buns and delicatessen by using auxiliary materials such as butter, sugar, and eggs, or a wide variety of ingredients and spices. Can be processed into bread.

本発明において生地とは、型に入れる前の1次発酵後のパン種を言う。 In the present invention, the dough refers to the leaven after the primary fermentation before being placed in a mold.

以下の実施例及び比較例により本発明をさらに詳しく説明するが、本発明は以下の実施例により何ら限定されるものではない。 The present invention will be described in more detail with reference to the following examples and comparative examples, but the present invention is not limited to the following examples.

<実施例1:発酵種の調製> <Example 1: Preparation of fermented seeds>

工程(A)
140mLの施栓可能な容器に、26gの米COBO(ウエダ家、自然発酵乳酸菌)、78gの水を量り入れた後に混合し、常温で1日静置して乳酸菌種を調製した。
Process (A)
26 g of rice COBO (Ueda family, naturally fermented lactic acid bacteria) and 78 g of water were weighed and mixed in a 140 mL capable container, and allowed to stand at room temperature for 1 day to prepare a lactic acid bacterium species.

工程(B)
225mLの施栓可能な容器に、104gの前記乳酸菌種、35gの紅花油を入れて栓をして撹拌し乳化させた後、冷蔵庫(7〜10℃)内で約24時間静置することにより乳化種を調製した。栓を開けると気体が抜ける音がし、油の粒が細かくなっていた。味見をすると甘みがあり、ほぼ油の感じはしなかった。
Process (B)
Put 104 g of the lactic acid bacterium species and 35 g of safflower oil in a 225 mL capable container, plug it, stir and emulsify it, and then leave it in the refrigerator (7 to 10 ° C) for about 24 hours to emulsify it. Seeds were prepared. When I opened the stopper, there was a sound of gas coming out, and the oil particles were fine. When I tasted it, it was sweet and almost not oily.

工程(C)
450mLの施栓可能な容器に、200gの甘酒(マルクラ食品、あま酒)、100gの水、68gの前記乳化種を入れて撹拌し後、施栓して冷蔵庫の野菜室(5〜8℃)内で約24時間静置することにより甘酒発酵種を調製した。開栓すると気体が抜ける音がした。
Process (C)
Put 200 g of amazake (Marukura food, sweet sake), 100 g of water, and 68 g of the emulsified seed in a 450 mL capable container, stir, plug and put in the vegetable compartment (5-8 ° C) of the refrigerator. A fermented amazake seed was prepared by allowing it to stand for about 24 hours. When I opened it, I heard the gas coming out.

<実施例2:甘酒発酵種を用いた生地の製造> <Example 2: Production of dough using fermented amazake seeds>

工程(D)
ボウルに、2240gの準強力粉(はるゆたか)、22gの塩、1560gの前記甘酒発酵種、224gの紅花油を入れた。
Process (D)
In a bowl, 2240 g of semi-strong flour (Haruyutaka), 22 g of salt, 1560 g of the fermented amazake seed and 224 g of safflower oil were placed.

工程(E)
室温を25℃、ボウル内容物の温度を28℃に保ちながら、前記ボウルの内容物をミキサー(愛工舎製作所、MS−30)で低速2分、中速2分で捏ねた後、224gの紅花油を入れ、さらに低速3分、中速6分、高速2分で捏ね上げ、発酵用の容器に入れた。
Process (E)
While keeping the room temperature at 25 ° C and the temperature of the bowl contents at 28 ° C, knead the contents of the bowl with a mixer (Aikosha Seisakusho, MS-30) at low speed for 2 minutes and medium speed for 2 minutes, and then 224 g of safflower. The oil was added, kneaded at low speed for 3 minutes, medium speed for 6 minutes, and high speed for 2 minutes, and placed in a fermentation container.

工程(F)
前記発酵用容器を、冷蔵庫(2〜4℃)内で、24〜36時間静置し、内容物を発酵させた(0次発酵)。
Process (F)
The fermentation vessel was allowed to stand in a refrigerator (2-4 ° C.) for 24 to 36 hours to ferment the contents (0th fermentation).

工程(G)
前記0次発酵物を、29℃に保ち、1〜2時間静置し、さらに発酵させ、生地を完成させた(1次発酵)。
Process (G)
The 0th-order fermented product was kept at 29 ° C., allowed to stand for 1 to 2 hours, and further fermented to complete the dough (primary fermentation).

<実施例3:パンの製造> <Example 3: Bread production>

工程(H)−1
前記生地(920g)を、ふわっと丸めて食パン型(山食)に入れて成形した。
Step (H) -1
The dough (920 g) was softly rolled and placed in a bread mold (mountain meal) for molding.

工程(I)−1
前記形成したパン生地を29℃に保ち、1時間40分静置し、内容物を発酵させた(2次発酵)。
Step (I) -1
The formed bread dough was kept at 29 ° C. and allowed to stand for 1 hour and 40 minutes to ferment the contents (secondary fermentation).

工程(J)−1
前記2次発酵させたパン生地をオーブン(上火180℃、下火250℃)に入れ、28分間焼成させて山型の食パンを得た。
Process (J) -1
The second-fermented bread dough was placed in an oven (top heat 180 ° C., bottom heat 250 ° C.) and baked for 28 minutes to obtain a mountain-shaped bread.

工程(H)−2
前記生地(400g)を、気泡をつぶして2回巻き、正方形角食型(角食)に入れて成形した。
Step (H) -2
The dough (400 g) was formed by crushing air bubbles, winding it twice, and placing it in a square eclipse mold (corner eclipse).

工程(I)−2
前記形成したパン生地を29℃に保ち、1時間15分静置し、内容物を発酵させた(2次発酵)。
Step (I) -2
The formed bread dough was kept at 29 ° C. and allowed to stand for 1 hour and 15 minutes to ferment the contents (secondary fermentation).

工程(J)−2
前記2次発酵させたパン生地をオーブン(上火210℃、下火230℃)に入れ、30分間焼成させて正方形の角食パンを得た。
Process (J) -2
The second-fermented bread dough was placed in an oven (top heat 210 ° C., bottom heat 230 ° C.) and baked for 30 minutes to obtain a square bread.

工程(H)−3
前記生地(450g)をきつく丸めてスリム角食型(角食)に入れて成形した。
Step (H) -3
The dough (450 g) was tightly rolled and placed in a slim square eclipse mold (square eclipse) for molding.

工程(I)−3
前記形成したパン生地を29℃に保ち、1時間40分静置し、内容物を発酵させた(2次発酵)。
Step (I) -3
The formed bread dough was kept at 29 ° C. and allowed to stand for 1 hour and 40 minutes to ferment the contents (secondary fermentation).

工程(J)−3
前記2次発酵させたパン生地をオーブン(上火180℃、下火250℃)に入れ、28分間焼成させてスリム角食パンを得た。
Process (J) -3
The second-fermented bread dough was placed in an oven (top heat 180 ° C., bottom heat 250 ° C.) and baked for 28 minutes to obtain slim square bread.

工程(H)−4
前記生地(460g)を、気泡をつぶして2回巻いて食パン小型(山食)に入れて成形した。
Process (H) -4
The dough (460 g) was formed by crushing air bubbles, rolling it twice, and putting it in a small loaf of bread (yamashoku).

工程(I)−4
前記形成したパン生地を29℃に保ち、1時間15分静置し、内容物を発酵させた(2次発酵)。
Step (I) -4
The formed bread dough was kept at 29 ° C. and allowed to stand for 1 hour and 15 minutes to ferment the contents (secondary fermentation).

工程(J)−4
前記2次発酵させたパン生地をオーブン(上火180℃、下火250℃)に入れ、25分間焼成させて小型の山型食パンを得た。
Process (J) -4
The second-fermented bread dough was placed in an oven (top heat 180 ° C., bottom heat 250 ° C.) and baked for 25 minutes to obtain a small mountain-shaped bread.

<風味・食感評価>
本発明の方法により製造されたパン(YAWARA)の香り、味、食感について、32人のモニターにより5段階で評価を行った。結果を表1に示す
<Evaluation of flavor and texture>
The aroma, taste, and texture of bread (YAWARA) produced by the method of the present invention were evaluated on a 5-point scale by monitors of 32 people. The results are shown in Table 1.

Figure 2021182878
Figure 2021182878

本発明により製造されたパン(YAWARA)は、小麦本来の香り(香ばしく、甘い香り)を有し、小麦本来のあまみやうまみを有することが分かった。さらに乳酸菌による保湿の働きで、しっとりとした食感を有することも分かった。 It was found that the bread (YAWARA) produced by the present invention has the original aroma of wheat (fragrant and sweet aroma) and has the original taste and umami of wheat. It was also found that the moisturizing action of lactic acid bacteria has a moist texture.

<保存期間の評価>
本発明の方法により製造された食パン(YAWARA)の常温、冷凍での保存状態を、市販の食パンの賞味期限(おいしく食べられる期限)、消費期限(安心して食べられる期限)とそれぞれ比較した。それぞれのパンは、乾燥を防ぐためにできるだけ空気に触れないようにしっかりとラップをするか、食パン専用の保存容器などを使用して保存した。結果を表2に示す。
<Evaluation of storage period>
The storage state of the bread (YAWARA) produced by the method of the present invention at room temperature and in freezing was compared with the best-by date (the time limit for delicious eating) and the expiration date (the time limit for eating with peace of mind) of commercially available bread. Each bread was wrapped tightly so that it would not come in contact with the air as much as possible to prevent it from drying, or it was stored in a storage container dedicated to bread. The results are shown in Table 2.

Figure 2021182878
Figure 2021182878

本発明により製造されたパン(YAWARA)は、保存剤等の添加物を使用していないのにもかかわらず、市販の食パンよりも長く保存できることが分かった。 It was found that the bread produced by the present invention (YAWARA) can be stored longer than commercially available bread, even though it does not use additives such as preservatives.

Claims (7)

自然発酵システムを利用したパン生地の製造方法であって、
(A)特定の菌叢を含む乳酸菌含有酵母基材、及び水を1:1〜1:9の重量比で混合して、5〜30℃で1〜10日間静置して、乳酸菌種を得る工程、
(B)施栓可能な容器に、前記乳酸菌種、及び植物性油脂を9:1〜1:1の重量比で入れて施栓し、撹拌し乳化させた後、5〜25℃で、20〜30時間静置することにより乳化種を得る工程、
(C)施栓可能な容器に、前記乳化種、及び天然物由来培地を1:1〜1:15の重量比で混合した後、施栓し5〜25℃で20〜30時間静置することにより発酵種を得る工程、
(D)前記発酵種、及び小麦粉を1:1〜1:4重量比で混合し、さらに前記小麦粉に対し、0.5〜2重量%の塩、及び9〜11重量%の植物性油脂を加えて混合物を得る工程、
(E)室温を16〜25℃、前記混合物の内部温度を26〜30℃に保ち、前記小麦粉に対し、9〜11重量%の植物性油脂を添加しながら、ミキサーで前記混合物を低速(50回/分〜200回/分の回転率)で、5秒〜20分間、中速(80回/分〜400回/分の回転率)で5秒〜20分間、高速(100回/分〜500回/分の回転率)で、5秒〜20分間捏ね上げてグルテンを出す工程、
(F)前記捏ね上げた混合物を発酵容器に入れ、2〜8℃の低温下で、24〜50時間静置し、内容物を発酵させ0次発酵物を得る工程(0次発酵)、及び
(G)前記0次発酵物を、20〜30℃で1〜8時間静置し、さらに発酵させる工程(1次発酵)、を含むパン生地の製造方法。
It is a method of manufacturing bread dough using a natural fermentation system.
(A) A lactic acid bacterium-containing yeast substrate containing a specific flora and water are mixed at a weight ratio of 1: 1 to 1: 9 and allowed to stand at 5 to 30 ° C. for 1 to 10 days to obtain lactic acid bacteria species. Getting process,
(B) The lactic acid bacterium species and vegetable oils and fats are placed in a capable container at a weight ratio of 9: 1 to 1: 1 and plugged, stirred and emulsified, and then 20 to 30 at 5 to 25 ° C. The process of obtaining an emulsified seed by allowing it to stand for a while,
(C) The emulsified seed and the natural product-derived medium are mixed in a cappable container at a weight ratio of 1: 1 to 1:15, then plugged and allowed to stand at 5 to 25 ° C. for 20 to 30 hours. The process of obtaining fermented seeds,
(D) The fermented seeds and wheat flour are mixed in a ratio of 1: 1 to 1: 4, and 0.5 to 2% by weight of salt and 9 to 11% by weight of vegetable fat and oil are further added to the flour. In addition, the process of obtaining the mixture,
(E) Keep the room temperature at 16 to 25 ° C. and the internal temperature of the mixture at 26 to 30 ° C., add 9 to 11% by weight of vegetable oil to the flour, and slow down (50) the mixture with a mixer. 5 seconds to 20 minutes at medium speed (80 times / minute to 400 times / minute rotation rate), 5 seconds to 20 minutes at medium speed (80 times / minute to 400 times / minute rotation rate), high speed (100 times / minute ~) A process of kneading for 5 to 20 minutes to produce gluten at a rotation rate of 500 times / minute.
(F) A step of putting the kneaded mixture in a fermentation vessel and allowing it to stand at a low temperature of 2 to 8 ° C. for 24 to 50 hours to ferment the contents to obtain a 0th-order fermentation product (0th-order fermentation). (G) A method for producing a bread dough, which comprises a step of allowing the 0th-order fermented product to stand at 20 to 30 ° C. for 1 to 8 hours and further fermenting it (primary fermentation).
前記乳酸菌含有酵母基材が、乳酸球菌と酵母の共生状態がみられ、かつ、乳酸球菌が優勢の状態を保ち、表面に「つや膜」が形成され、非加熱で雑菌が不検出(1万個以下/g)の菌叢を含む、請求項1に記載のパン生地の製造方法。 The lactic acid bacterium-containing yeast substrate shows a symbiotic state of lactic acid bacterium and yeast, and the lactic acid bacterium remains predominant, a "glossy film" is formed on the surface, and no germs are detected without heating (10,000). The method for producing bread dough according to claim 1, which comprises a bacterial flora of 100 pieces or less / g). 前記小麦粉が、9〜11.5%のタンパク質を含有する、請求項1又は2に記載のパン生地の製造方法。 The method for producing a bread dough according to claim 1 or 2, wherein the flour contains 9 to 11.5% protein. 前記天然物由来培地が、穀物由来物、植物由来物、又は乳由来物を含む、請求項1〜3のいずれか一項に記載のパン生地の製造方法。 The method for producing a bread dough according to any one of claims 1 to 3, wherein the natural product-derived medium contains a grain-derived product, a plant-derived product, or a milk-derived product. 自然発酵システムを利用したパン類の製造方法であって
(H)請求項1〜4のいずれか一項に記載の製造方法により得られたパン生地を型に入れ成形する工程、
(I)前記成形したパン生地を28〜30℃に保ち、0.75〜3時間静置し発酵させ2次発酵物を得る工程(2次発酵)、及び
(J)前記2次発酵物を、上火160〜230℃、下火210〜270℃のオーブンで、15〜40分間焼成する工程を含む、パン類の製造方法。
A method for producing bread using a natural fermentation system, wherein the bread dough obtained by the production method according to any one of claims 1 to 4 is placed in a mold and molded.
(I) The step of keeping the molded bread dough at 28 to 30 ° C. and allowing it to stand for 0.75 to 3 hours to ferment to obtain a secondary fermentation product (secondary fermentation), and (J) the secondary fermentation product. A method for producing bread, which comprises a step of baking for 15 to 40 minutes in an oven having a top heat of 160 to 230 ° C and a bottom heat of 210 to 270 ° C.
請求項1〜4のいずれか一項に記載の方法により製造されたパン生地。 A bread dough produced by the method according to any one of claims 1 to 4. 請求項5に記載の方法により製造されたパン類。 Breads produced by the method according to claim 5.
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