JP2021172635A - Plant growth promoter - Google Patents
Plant growth promoter Download PDFInfo
- Publication number
- JP2021172635A JP2021172635A JP2020080169A JP2020080169A JP2021172635A JP 2021172635 A JP2021172635 A JP 2021172635A JP 2020080169 A JP2020080169 A JP 2020080169A JP 2020080169 A JP2020080169 A JP 2020080169A JP 2021172635 A JP2021172635 A JP 2021172635A
- Authority
- JP
- Japan
- Prior art keywords
- gluconate
- plant
- plant growth
- betaine
- growth promoter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 230000008635 plant growth Effects 0.000 title claims abstract description 78
- 239000007952 growth promoter Substances 0.000 title claims abstract description 61
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims abstract description 106
- 229960003237 betaine Drugs 0.000 claims abstract description 46
- 229910052751 metal Inorganic materials 0.000 claims abstract description 46
- 239000002184 metal Substances 0.000 claims abstract description 46
- RGHNJXZEOKUKBD-SQOUGZDYSA-M D-gluconate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O RGHNJXZEOKUKBD-SQOUGZDYSA-M 0.000 claims abstract description 38
- 229940050410 gluconate Drugs 0.000 claims abstract description 37
- 239000013522 chelant Substances 0.000 claims abstract description 29
- 239000004480 active ingredient Substances 0.000 claims abstract description 8
- WHMDKBIGKVEYHS-IYEMJOQQSA-L Zinc gluconate Chemical compound [Zn+2].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O WHMDKBIGKVEYHS-IYEMJOQQSA-L 0.000 claims description 35
- RGHNJXZEOKUKBD-SQOUGZDYSA-N Gluconic acid Natural products OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 claims description 32
- 239000011670 zinc gluconate Substances 0.000 claims description 26
- 235000011478 zinc gluconate Nutrition 0.000 claims description 26
- 229960000306 zinc gluconate Drugs 0.000 claims description 26
- OCUCCJIRFHNWBP-IYEMJOQQSA-L Copper gluconate Chemical compound [Cu+2].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O OCUCCJIRFHNWBP-IYEMJOQQSA-L 0.000 claims description 25
- 229940108925 copper gluconate Drugs 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 21
- 150000003839 salts Chemical class 0.000 claims description 18
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 claims description 16
- 239000000174 gluconic acid Substances 0.000 claims description 16
- 235000012208 gluconic acid Nutrition 0.000 claims description 16
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 9
- 238000012364 cultivation method Methods 0.000 claims description 9
- 239000010949 copper Substances 0.000 claims description 8
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 7
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 7
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- AEQDJSLRWYMAQI-UHFFFAOYSA-N 2,3,9,10-tetramethoxy-6,8,13,13a-tetrahydro-5H-isoquinolino[2,1-b]isoquinoline Chemical compound C1CN2CC(C(=C(OC)C=C3)OC)=C3CC2C2=C1C=C(OC)C(OC)=C2 AEQDJSLRWYMAQI-UHFFFAOYSA-N 0.000 claims description 3
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Abstract
Description
本発明は植物生育促進剤に関する。 The present invention relates to a plant growth promoter.
植物の生育を促進させる技術は、農作物の早期収穫や植物バイオマス生産量の増加等、農林業上、極めて重要である。また、近年の新興国の経済成長に伴う生活水準の向上や中長期的な人口増加により、世界の食糧需要は上昇傾向が予測されている。そのため効率的で安全、かつ安価な植物生育方法の開発は急務となっている。 Techniques for promoting plant growth are extremely important in agriculture and forestry, such as early harvesting of crops and increase in plant biomass production. In addition, global food demand is expected to rise due to the improvement of living standards and medium- to long-term population growth accompanying the economic growth of emerging countries in recent years. Therefore, there is an urgent need to develop an efficient, safe and inexpensive plant growth method.
それ故に、当該分野では、栽培条件の最適化、肥料や生育促進剤の開発、ノックアウト植物及びトランスジェニック植物の作製等、上記課題を解決するための様々な試みがなされてきた。 Therefore, in this field, various attempts have been made to solve the above problems, such as optimization of cultivation conditions, development of fertilizers and growth promoters, production of knockout plants and transgenic plants.
本発明は、植物の生育を促進することのできる安全性の高い新たな薬剤を開発し、提供することである。 The present invention is to develop and provide a new highly safe drug capable of promoting the growth of plants.
グルコン酸金属キレートは、近年、植物ウイルスに対する防除効果が明らかになっている（特開2017-178838）。一般的なグルコン酸金属キレートには、グルコン酸銅やグルコン酸亜鉛が挙げられる。銅、及び亜鉛は、植物の生育上不可欠な元素であり、それらの欠乏により植物は生育が抑制される、又は停止することが知られている。それを回避するために、銅や亜鉛は肥料や植物活力剤としても広く利用されている。 In recent years, metal gluconate chelate has been clarified as having a control effect on plant viruses (Japanese Patent Laid-Open No. 2017-178838). Common metal gluconate chelates include copper gluconate and zinc gluconate. Copper and zinc are essential elements for the growth of plants, and it is known that their deficiency suppresses or stops the growth of plants. To avoid this, copper and zinc are also widely used as fertilizers and plant vitalizers.
そこで、本発明者は、グルコン酸金属キレートが植物ウイルス防除効果に加え、植物生育促進効果を有することを期待し、上記課題解決のためグルコン酸金属キレートを用いた新規植物生育促進剤の研究開発を行った。しかし、グルコン酸金属キレート単独では、僅かながらの生育促進効果が見られるに過ぎなかった。 Therefore, the present inventor expects that the metal gluconate chelate has a plant growth promoting effect in addition to the plant virus control effect, and research and development of a new plant growth promoting agent using the gluconate metal chelate to solve the above problems. Was done. However, the metal gluconate chelate alone showed only a slight growth promoting effect.
ところが、グルコン酸金属キレートをベタインと組み合わせて植物に施用した場合、それぞれを単独で使用したときの効果を顕著に上回る植物生育促進効果が現れることが明らかとなった。ベタインは、植物の根毛の発生を促進し、根量を増加させる等植物の地下部の生育に対する促進効果があることが知られているが、地上部の生育に対する促進効果は見られない（特開平１-208386、特開平１-228416）。一方、グルコン酸金属キレートとベタインの組み合わせでは植物の地上部及び地下部のいずれの生育促進効果も見られた。本発明は、当該知見に基づくものであって、以下を提供する。 However, it was clarified that when the metal gluconate chelate was applied to a plant in combination with betaine, a plant growth promoting effect significantly exceeding the effect when each of them was used alone was exhibited. Betaine is known to have a promoting effect on the growth of the underground part of the plant, such as promoting the growth of root hair of the plant and increasing the amount of roots, but no promoting effect on the growth of the above-ground part is observed (special feature). Kaihei 1-208386, JP-A 1-228416). On the other hand, the combination of the metal gluconate chelate and betaine was found to have a growth-promoting effect on both the above-ground part and the underground part of the plant. The present invention is based on this finding and provides the following.
（１）グルコン酸金属キレート及びベタインを有効成分として含む植物生育促進剤。
（２）前記グルコン酸金属キレートがグルコン酸銅、グルコン酸亜鉛、及びグルコン酸鉄からなる群から選択される１以上の金属キレートである、（１）に記載の植物生育促進剤。
（３）グルコン酸及び／又はグルコン酸塩、並びに無機金属塩をさらに含む、（１）又は（２）に記載の植物生育促進剤。
（４）前記グルコン酸塩がグルコン酸ナトリウム、グルコン酸カリウム、及びグルコン酸カルシウムからなる群から選択される１以上の塩であり、前記無機金属塩が無機銅、無機亜鉛、及び無機鉄からなる群から選択される１以上の塩である、（３）に記載の植物生育促進剤。
（５）前記グルコン酸金属キレートの含有量が0.1ｍM〜50ｍMである、（１）〜（４）のいずれかに記載の植物生育促進剤。
（６）前記ベタインの含有量が10ppm〜500ppmである、（１）〜（５）のいずれかに記載の植物生育促進剤。
（７）植物栽培方法であって、（１）〜（６）のいずれかに記載の植物生育促進剤を対象植物に施用する施用工程、及び該対象植物を栽培する栽培工程を含む前記方法。
(1) A plant growth promoter containing a metal gluconate chelate and betaine as active ingredients.
(2) The plant growth promoter according to (1), wherein the metal gluconate chelate is one or more metal chelates selected from the group consisting of copper gluconate, zinc gluconate, and iron gluconate.
(3) The plant growth promoter according to (1) or (2), further comprising gluconic acid and / or gluconate, and an inorganic metal salt.
(4) The gluconate salt is one or more salts selected from the group consisting of sodium gluconate, potassium gluconate, and calcium gluconate, and the inorganic metal salt is composed of inorganic copper, inorganic zinc, and inorganic iron. The plant growth promoter according to (3), which is one or more salts selected from the group.
(5) The plant growth promoter according to any one of (1) to (4), wherein the content of the metal gluconate chelate is 0.1 mM to 50 mM.
(6) The plant growth promoter according to any one of (1) to (5), wherein the betaine content is 10 ppm to 500 ppm.
(7) The method for cultivating a plant, which comprises an application step of applying the plant growth promoter according to any one of (1) to (6) to a target plant, and a cultivation step of cultivating the target plant.
本発明の植物生育促進剤によれば、植物への施用が認められている2つの薬剤を用いることで、新規の植物生育促進剤を提供することができる。 According to the plant growth promoter of the present invention, a novel plant growth promoter can be provided by using two agents that are approved for application to plants.
１．植物生育促進剤
１−１．概要
本発明の第1の態様は、植物生育促進剤である。本発明の植物生育促進剤は、構成成分としてグルコン酸金属キレート及びベタインを含む組成物である。本発明の植物生育促進剤によれば、植物地上部及び地下部の両者の生育を促進することができる。
1. 1. Plant growth promoter 1-1. Overview The first aspect of the present invention is a plant growth promoter. The plant growth promoter of the present invention is a composition containing a metal gluconate chelate and betaine as constituent components. According to the plant growth promoter of the present invention, it is possible to promote the growth of both the above-ground part and the underground part of the plant.
１−２．定義
本明細書で頻用する用語について、以下で定義する。
本明細書において「植物（の）生育」とは、植物体の重量、好ましくは乾燥重量の増加をいい、具体的には、植物体の伸長及び／又は拡大、葉数及び／又は茎数の増加、花芽形成、結実等が該当する。
1-2. Definitions The terms frequently used herein are defined below.
As used herein, the term "plant growth" refers to an increase in the weight of a plant, preferably a dry weight, specifically, the elongation and / or expansion of the plant, the number of leaves and / or the number of stems. Increase, flower bud formation, fruiting, etc. are applicable.
「植物生育促進（作用）」とは、対照植物と比較して、生育を促進すること、又はその作用をいう。これにより、同環境下において同期間で栽培した対照植物よりも多くのバイオマスを得ることができる。ここでいう「対照植物」とは、本発明の植物生育促進剤を施用する対象植物と同種の植物であって、本発明の植物生育促進剤が非施用であることを除いて同一条件下で栽培される植物をいう。 "Plant growth promotion (action)" refers to promoting growth or its action as compared with a control plant. As a result, more biomass can be obtained than the control plants cultivated in the same environment in the same environment. The "control plant" here is a plant of the same species as the target plant to which the plant growth promoter of the present invention is applied, and under the same conditions except that the plant growth promoter of the present invention is not applied. A plant that is cultivated.
本明細書において「施用」とは、植物に対して薬剤や肥料等を投与し、使用することをいう。 As used herein, the term "application" refers to the administration and use of chemicals, fertilizers, etc. to plants.
本明細書において「（対象）植物」とは、本発明の植物生育促進剤の施用対象となる植物であり、また後述する第２態様における植物栽培方法において栽培対象となる植物をいう。植物の種類は問わない。被子植物又は裸子植物のいずれであってもよい。さらに、草本植物及び木本植物も問わない。本発明の植物生育促進剤の施用対象植物の具体例としては、農業上重要な植物、例えば、穀類、野菜、果物等の作物植物や花卉植物が挙げられる。具体的には、単子葉植物では、イネ科（Poaceae）植物（例えば、イネ、コムギ、オオムギ、トウモロコシ、サトウキビ、ソルガム、コウリャン、芝草（例えば、Zoysia, Cynodon, Pao, Agrostis, Festuca, Lolium等に属する種））が挙げられる。また、双子葉植物では、アブラナ科（Brassicaceae）植物（例えば、キャベツ、ダイコン、ハクサイ、アブラナ）、キク科（Asteraceae）植物（例えば、レタス、ゴボウ、キク）、マメ科（Fabaceae）植物（例えば、ダイズ、落花生、エンドウ、インゲンマメ、レンズマメ、ヒヨコマメ、ソラマメ）、ナス科（Solanaceae）植物（例えば、トマト、ナス、ジャガイモ、タバコ、ピーマン、トウガラシ、ペチュニア）、バラ科（Rosaceae）植物（例えば、イチゴ、リンゴ、ナシ、モモ、ビワ、アーモンド、スモモ、バラ、ウメ、サクラ）、ウリ科（Cucurbitaceae）植物（例えば、キュウリ、ウリ、カボチャ、メロン、スイカ）、ユリ科（Liliaceae）植物（例えば、ネギ、タマネギ、ユリ）、ミカン科（Rutaceae）植物（例えば、ミカン、グレープフルーツ、レモン、ユズ）、ヒルガオ科（Convolvulaceae）植物（例えば、サツマイモ）、ツバキ科（Theaceae）植物（例えばチャノキ）、及びブドウ科（Vitaceae）植物（例えば、ブドウ）が該当する。 In the present specification, the "(target) plant" refers to a plant to which the plant growth promoter of the present invention is applied, and a plant to be cultivated in the plant cultivation method according to the second aspect described later. The type of plant does not matter. It may be either angiosperms or gymnosperms. Furthermore, herbaceous plants and woody plants are not limited. Specific examples of the plant to which the plant growth promoter of the present invention is applied include agriculturally important plants such as crop plants such as grains, vegetables and fruits, and flower plants. Specifically, for monocotyledonous plants, grasses (Poaceae) plants (eg, rice, wheat, cynodon, corn, sugar cane, sorghum, kouryan, turfgrass (eg, Zoysia, Cynodon, Pao, Agrostis, Festuca, Lolium, etc.) Species to which it belongs)). In addition, among dicotyledonous plants, Brassicaceae plants (eg, cabbage, daikon, hakusai, abrana), Asteraceae plants (eg, lettuce, gobo, kiku), Fabaceae plants (eg, Fabaceae) plants (eg,) Soybeans, peanuts, peas, green beans, lens beans, chicks, solanaceae plants (eg, tomatoes, eggplants, potatoes, tobacco, peppers, capsicum, petunia), roses (rosaceae) plants (eg strawberries, Apples, pears, peaches, biwa, almonds, peaches, roses, sea urchins, cherry blossoms), Cucurbitaceae plants (eg cucumbers, uris, pumpkins, melons, watermelons), Liliaceae plants (eg onions, Onions, lilies, Rutaceae plants (eg, citrus, grapefruit, lemon, yuzu), Convolvulaceae plants (eg, sweet potatoes), Theaceae plants (eg, Chanoki), and grapes (eg, Chanoki). Vitaceae) Plants (eg grapes) are applicable.
１−３．構成
１−３−１．構成成分
本発明の植物生育促進剤は、グルコン酸金属キレート及びベタインを必須の構成成分として含む。このグルコン酸金属キレート及びベタインが本発明の生育促進剤における有効成分となり得る。さらに必要に応じて、グルコン酸及び／又はグルコン酸塩、並びに無機金属塩、そして農学上許容可能な担体を含むことができる。以下、それぞれの構成成分について、具体的に説明をする。
1-3. Configuration 1-3-1. Components The plant growth promoter of the present invention contains a metal gluconate chelate and betaine as essential components. The metal gluconate chelate and betaine can be active ingredients in the growth promoter of the present invention. Further, if desired, gluconic acid and / or gluconate, as well as inorganic metal salts, and an agronomically acceptable carrier can be included. Hereinafter, each component will be specifically described.
（１）グルコン酸
「グルコン酸（C6H12O7）」は、グルコースにおける1位の炭素が酸化されピラノース環が開裂したカルボン酸で、強力なキレート剤として知られている。自然界にも存在し、ハチミツやローヤルゼリー等の食品にも含まれる植物や人体に安全な低分子化合物である。本発明の植物生育促進剤では、限定はしないが、通常、水に溶解した状態（グルコン酸水溶液）で存在し得る。したがって、水に溶解することで加水分解によってグルコン酸となるグルコノラクトンも本明細書においては、グルコン酸に包含される。グルコン酸は、本発明の植物生育促進剤における選択的構成成分であり、必要に応じて添加すればよい。
(1) Gluconic acid "Gluconic acid (C 6 H 12 O 7 )" is a carboxylic acid in which the carbon at the 1-position in glucose is oxidized and the pyranose ring is cleaved, and is known as a strong chelating agent. It is a small molecule compound that exists in nature and is safe for plants and the human body, which is also contained in foods such as honey and royal jelly. The plant growth promoter of the present invention is not limited, but can usually exist in a state of being dissolved in water (gluconic acid aqueous solution). Therefore, gluconolactone, which becomes gluconic acid by hydrolysis when dissolved in water, is also included in gluconic acid in the present specification. Gluconic acid is a selective constituent of the plant growth promoter of the present invention and may be added as needed.
（２）グルコン酸金属キレート
「グルコン酸金属キレート」とは、グルコン酸が重金属をキレート配位した化合物である。グルコン酸金属キレートの種類は限定しないが、配位する金属が植物生育上不可欠な元素であることが好ましい。例えば、グルコン酸銅、グルコン酸亜鉛、グルコン酸鉄等が挙げられる。本発明の植物生育促進剤は、1種、又は2種以上のグルコン酸金属キレートを含むことができる。例えば、前述のグルコン酸銅、グルコン酸亜鉛、グルコン酸鉄をそれぞれ単独で含んでいてもよいし、グルコン酸銅とグルコン酸鉄、グルコン酸亜鉛とグルコン酸鉄、グルコン酸銅とグルコン酸亜鉛、並びにグルコン酸銅、グルコン酸亜鉛、及びグルコン酸鉄等の組み合わせにより2種以上のグルコン酸金属キレートを含んでいてもよい。
(2) Metal gluconate chelate “Metal gluconate chelate” is a compound in which gluconic acid chelate a heavy metal. The type of metal gluconate chelate is not limited, but it is preferable that the metal to be coordinated is an essential element for plant growth. For example, copper gluconate, zinc gluconate, iron gluconate and the like can be mentioned. The plant growth promoter of the present invention may contain one kind or two or more kinds of metal gluconate chelate. For example, the above-mentioned copper gluconate, zinc gluconate, and iron gluconate may be contained alone, or copper gluconate and iron gluconate, zinc gluconate and iron gluconate, copper gluconate and zinc gluconate, In addition, two or more kinds of metal gluconate chelate may be contained by a combination of copper gluconate, zinc gluconate, iron gluconate and the like.
植物生育促進剤におけるグルコン酸金属キレートの含有量は、限定はしないが、施用時の終濃度が0.1mM〜50mMの範囲にあればよい。例えば、グルコン酸銅を0.1mM〜5mM、0.2mM〜10mM、0.5mM〜20mM、及び1mM〜40mMの範囲とすることができる。グルコン酸金属キレートを複数種包含する場合、それぞれのグルコン酸金属キレートの含有量は同一であってもよいし、異なっていてもよい。ただし、施用前の植物生育促進剤は濃縮状態であってもよく、施用時に水、バッファー等で希釈することもできる。この場合、植物生育促進剤に含まれるグルコン酸金属キレートの含有量は前記終濃度の範囲を超えていてもよい。その場合、施用時の終濃度を濃縮率で乗じた濃度を含むことができる。濃縮率は限定しないが、例えば、5倍〜1000倍、10〜800倍、100〜500倍の範囲であればよい。 The content of the metal gluconate chelate in the plant growth promoter is not limited, but the final concentration at the time of application may be in the range of 0.1 mM to 50 mM. For example, copper gluconate can be in the range of 0.1 mM to 5 mM, 0.2 mM to 10 mM, 0.5 mM to 20 mM, and 1 mM to 40 mM. When a plurality of types of metal gluconate chelates are included, the content of each metal gluconate chelate may be the same or different. However, the plant growth promoter before application may be in a concentrated state, and may be diluted with water, buffer or the like at the time of application. In this case, the content of the metal gluconate chelate contained in the plant growth promoter may exceed the range of the final concentration. In that case, it can include the concentration obtained by multiplying the final concentration at the time of application by the concentration rate. The concentration rate is not limited, but may be in the range of, for example, 5 times to 1000 times, 10 to 800 times, and 100 to 500 times.
（３）ベタイン
「ベタイン」とは、植物や海産物等に含有される形で自然界に広く存在する物質であり、構造的には、同一分子内の隣接しない位置に負電荷及び解離水素が結合しない正電荷を有し、分子全体として電荷を持たない分子内塩としての特徴を有する。本発明の植物生育促進剤におけるベタインの種類は問わないが、入手の容易性や安全性から、生体物質としてのベタインが好ましい。例えば、グリシンベタイン（トリメチルグリシン）、プロリンベタイン、カルニチン等が挙げられる。本発明の植物生育促進剤は、1種、又は2種以上のベタインを含むことができる。例えば、前述の、グリシンベタイン、プロリンベタイン、及びカルニチンをそれぞれ単独で含んでいてもよいし、グリシンベタインとプロリンベタイン、グリシンベタインとカルニチン、プロリンベタインとカルニチン、及びグリシンベタイン、プロリンベタイン及びカルニチン等の組み合わせにより2種以上のベタインを含んでいてもよい。
(3) Betaine "Betaine" is a substance that is widely present in nature in the form of being contained in plants, marine products, etc., and structurally, negative charges and dissociated hydrogen do not bond to non-adjacent positions within the same molecule. It has the characteristics of an intramolecular salt having a positive charge and no charge as a whole molecule. The type of betaine in the plant growth promoter of the present invention is not limited, but betaine as a biological substance is preferable from the viewpoint of availability and safety. For example, glycine betaine (trimethylglycine), proline betaine, carnitine and the like can be mentioned. The plant growth promoter of the present invention may contain one or more betaines. For example, the above-mentioned glycine betaine, proline betaine, and carnitine may be contained alone, or glycine betaine and proline betaine, glycine betaine and carnitine, proline betaine and carnitine, and glycine betaine, proline betaine, carnitine, and the like. Depending on the combination, two or more kinds of betaines may be contained.
植物生育促進剤におけるベタインの含有量は、限定はしないが、施用時の終濃度が10ppm〜500ppmの範囲にあればよい。例えば、グリシンベタインを10ppm〜100ppm、30ppm〜200ppm、50ppm〜300ppm、及び80ppm〜400ppmの範囲とすることができる。ベタインを複数種包含する場合、それぞれのベタインの含有量は同一であってもよいし、異なっていてもよい。ただし、施用前の植物生育促進剤は濃縮状態であってもよく、この場合、植物生育促進剤に含まれるベタインの含有量は前記終濃度の範囲を超えていてもよい。その場合、上記グルコン酸金属キレートと同様に、施用時の終濃度を濃縮率で乗じた濃度を含むことができる。 The content of betaine in the plant growth promoter is not limited, but the final concentration at the time of application may be in the range of 10 ppm to 500 ppm. For example, glycine betaine can be in the range of 10 ppm to 100 ppm, 30 ppm to 200 ppm, 50 ppm to 300 ppm, and 80 ppm to 400 ppm. When a plurality of betaines are included, the content of each betaine may be the same or different. However, the plant growth promoter before application may be in a concentrated state, and in this case, the content of betaine contained in the plant growth promoter may exceed the range of the final concentration. In that case, similarly to the above-mentioned metal gluconate chelate, the concentration obtained by multiplying the final concentration at the time of application by the concentration rate can be included.
（４）グルコン酸塩
「グルコン酸塩」とは、グルコン酸の塩類である。グルコン酸塩は、本発明の植物生育促進剤における選択的構成成分であり、必要に応じて添加される。
グルコン酸塩の具体例としては、グルコン酸ナトリウム、グルコン酸カリウム、グルコン酸カルシウム等が挙げられるが、これに限定はされない。グルコン酸塩は、本発明の植物生育促進剤中に１種、又は２種以上含まれていてもよい。
(4) Gluconic acid "Gluconic acid" is a salt of gluconic acid. Gluconic acid is a selective constituent of the plant growth promoter of the present invention and is added as needed.
Specific examples of the gluconate include, but are not limited to, sodium gluconate, potassium gluconate, calcium gluconate and the like. Gluconic acid salt may be contained alone or in combination of two or more in the plant growth promoter of the present invention.
（５）無機金属塩
「無機金属塩」は、本発明の植物生育促進剤における選択的構成成分であり、硫酸塩、リン酸塩、硝酸塩、酢酸塩、塩化物、炭酸塩等が該当する。無機金属塩における金属元素の種類は限定しないが植物生育上不可欠な元素であることが好ましい。例えば、銅、亜鉛、鉄等が挙げられる。したがって、好ましい無機金属塩は、無機銅、無機亜鉛、及び無機鉄である。具体的には、硫酸銅、硫酸亜鉛、硫酸鉄、リン酸銅、リン酸亜鉛、リン酸鉄、硝酸銅、硝酸亜鉛、硝酸鉄、塩化銅、塩化亜鉛、塩化鉄、酢酸銅、酢酸亜鉛、酢酸鉄、炭酸銅、炭酸亜鉛、炭酸鉄等が挙げられる。無機金属塩は、本発明の植物生育促進剤中に１種、又は２種以上含まれていてもよい。
(5) Inorganic metal salt The "inorganic metal salt" is a selective constituent component in the plant growth promoter of the present invention, and corresponds to sulfates, phosphates, nitrates, acetates, chlorides, carbonates and the like. The type of metal element in the inorganic metal salt is not limited, but it is preferably an element indispensable for plant growth. For example, copper, zinc, iron and the like can be mentioned. Therefore, preferred inorganic metal salts are inorganic copper, inorganic zinc, and inorganic iron. Specifically, copper sulfate, zinc sulfate, iron sulfate, copper phosphate, zinc phosphate, iron phosphate, copper nitrate, zinc nitrate, iron nitrate, copper chloride, zinc chloride, iron chloride, copper acetate, zinc acetate, Examples thereof include iron acetate, copper carbonate, zinc carbonate and iron carbonate. The inorganic metal salt may be contained alone or in combination of two or more in the plant growth promoter of the present invention.
本発明の植物生育促進剤において無機金属塩と前記グルコン酸及び／又はグルコン酸塩は、組み合わせて添加することが望ましい。これは、無機金属塩とグルコン酸又はグルコン酸塩を水中で溶解し、キレート化させることで、有効成分であるグルコン酸金属キレートを新たに生成できるからである。 In the plant growth promoter of the present invention, it is desirable to add the inorganic metal salt and the gluconic acid and / or gluconate in combination. This is because the metal gluconate chelate, which is an active ingredient, can be newly produced by dissolving the inorganic metal salt and gluconic acid or gluconate in water and chelating them.
（６）農学上許容可能な担体
本発明の植物生育促進剤は、選択的な構成要素として、必要に応じて農学上許容可能な担体を含むことができる。
「農学上許容可能」とは、本発明の植物生育促進剤の施用を容易にし、その分解を阻害若しくは抑制し、及び／又はその作用速度を制御する物質であって、野外及び屋内において植物の栽培に施用しても、土壌及び水質等の環境に対する有害な影響がないか又は小さく、かつ動物、特にヒトに対する有害性がないか又は低い性質を有していることをいう。
(6) Agriculturally Acceptable Carrier The plant growth promoter of the present invention may optionally include an agriculturally acceptable carrier as a selective component.
"Agriculturally acceptable" is a substance that facilitates the application of the plant growth promoter of the present invention, inhibits or suppresses its decomposition, and / or controls its rate of action, and is used for plants in the field and indoors. It means that even if it is applied to cultivation, it has no or small harmful effects on the environment such as soil and water quality, and has no or low harmful effects on animals, especially humans.
「担体」は、例えば、溶剤及び補助剤が該当する。 The "carrier" corresponds to, for example, a solvent and an auxiliary agent.
「溶媒」には、水、芳香族化合物溶媒(例えば、ベンゼン、トルエン、キシレン、テトラヒドロナフタレン、アルキル化ナフタレン若しくはその誘導体)、パラフィン類（例えば、鉱油留分）、クロロホルム、四塩化炭素、ケトン類（例えば、アセトン、シクロヘキサノン）、ピロリドン類(例えば、NMP又はNOP)、アセテート(二酢酸グリコール)、グリコール類、脂肪酸ジメチルアミド類、脂肪酸類、脂肪酸エステル類、又はそれらの混合溶媒が挙げられる。 "Solvents" include water, aromatic compound solvents (eg, benzene, toluene, xylene, tetrahydronaphthalene, alkylated naphthalene or derivatives thereof), paraffins (eg, mineral oil distillates), chloroform, carbon tetrachloride, ketones. (For example, acetone, cyclohexanone), pyrrolidones (for example, NMP or NOP), acetate (glycol diacetate), glycols, fatty acid dimethylamides, fatty acids, fatty acid esters, or a mixed solvent thereof.
「補助剤」としては、粉砕天然鉱物、粉砕合成鉱物、乳化剤、分散剤、展着剤及び界面活性剤等が挙げられる。 Examples of the "auxiliary agent" include crushed natural minerals, crushed synthetic minerals, emulsifiers, dispersants, spreading agents, surfactants and the like.
粉砕天然鉱物には、例えば、カオリン、クレイ、タルク及びチョークが挙げられる。 Grinded natural minerals include, for example, kaolin, clay, talc and chalk.
粉砕合成鉱物には、例えば、高分散シリカ及びシリケートが挙げられる。乳化剤としては、非イオン性乳化剤やアニオン性乳化剤（例えば、ポリオキシエチレン脂肪アルコールエーテル、アルキルスルホネート及びアリールスルホネート）が挙げられる。 Examples of the pulverized synthetic mineral include highly dispersed silica and silicate. Examples of the emulsifier include nonionic emulsifiers and anionic emulsifiers (eg, polyoxyethylene fatty alcohol ethers, alkyl sulfonates and aryl sulfonates).
分散剤には、例えば、リグノ亜硫酸廃液及びメチルセルロースが挙げられる。 Dispersants include, for example, lignosulfurous acid effluent and methylcellulose.
界面活性剤には、例えば、リグノスルホン酸、ナフタレンスルホン酸、フェノールスルホン酸、ジブチルナフタレンスルホン酸のアルカリ金属塩、アルカリ土類金属塩及びアンモニウム塩、アルキルアリールスルホネート、アルキルスルフェート、アルキルスルホネート、脂肪アルコールスルフェート、脂肪酸及び硫酸化脂肪アルコールグリコールエーテル、さらに、スルホン化ナフタレン及びナフタレン誘導体とホルムアルデヒドの縮合物、ナフタレン又はナフタレンスルホン酸とフェノール及びホルムアルデヒドの縮合物、ポリオキシエチレンオクチルフェニルエーテル、エトキシル化イソオクチルフェノール、オクチルフェノール、ノニルフェノール、アルキルフェニルポリグリコールエーテル、トリブチルフェニルポリグリコールエーテル、トリステアリルフェニルポリグリコールエーテル、アルキルアリールポリエーテルアルコール、アルコール及び脂肪アルコール／エチレンオキシドの縮合物、エトキシル化ヒマシ油、ポリオキシエチレンアルキルエーテル、ポリオキシエチレンヘキシタン脂肪酸エステル、ソルビタン脂肪酸エステル、エトキシル化ポリオキシプロピレン、ラウリルアルコールポリグリコールエーテルアセタール、ソルビトールエステル、リグノ亜硫酸廃液、及びメチルセルロースが挙げられる。 Surfactants include, for example, lignosulfonic acid, naphthalenesulfonic acid, phenolsulfonic acid, alkali metal salts of dibutylnaphthalenesulfonic acid, alkaline earth metal salts and ammonium salts, alkylarylsulfonates, alkylsulfates, alkylsulfonates, fats. Alcohol sulfate, fatty acids and sulfated fatty alcohol glycol ethers, sulfonated naphthalenes and naphthalene derivatives and formaldehyde condensates, naphthalene or naphthalene sulfonic acid plus phenol and formaldehyde condensates, polyoxyethylene octylphenyl ethers, ethoxylated iso Octylphenol, octylphenol, nonylphenol, alkylphenylpolyglycol ether, tributylphenylpolyglycol ether, tristearylphenylpolyglycol ether, alkylarylpolyether alcohol, alcohol and fatty alcohol / ethylene oxide condensates, ethoxylated castor oil, polyoxyethylene alkyl Examples include ether, polyoxyethylene hexitane fatty acid ester, sorbitan fatty acid ester, ethoxylated polyoxypropylene, lauryl alcohol polyglycol ether acetal, sorbitol ester, ligno sulfite waste liquid, and methyl cellulose.
（７）その他
本発明の植物生育促進剤は、必要に応じて他の作用を有する成分、例えば、栄養素、成長強化剤、殺菌剤、殺虫剤等を包含することもできる。「栄養素」とは、その物質の欠乏により植物が成長上又は生殖上何らかの異常をきたし、その症状の回復が他の物質の供給では補償できない物質をいう。原則として植物の必須元素を意味する。一般的な植物の必須元素としては、17種の元素、すなわち、水素（H）、酸素（O）、炭素（C）、窒素（N）、リン（P）、カリウム（K）、マグネシウム（Mg）、カルシウム（Ca）、硫黄（S）、鉄（Fe）、マンガン（Mg）、亜鉛（Zn）、ホウ素（B）、モリブデン（Mo）、銅（Cu）、塩素（Cl）、及びニッケル（Ni）が挙げられる。また、それらの元素を含む肥料（例えば、尿素、アンモニウム塩、(過)リン酸塩）もここでいう栄養素に含まれる。
(7) Others The plant growth promoter of the present invention may also include components having other actions, such as nutrients, growth enhancers, fungicides, insecticides, etc., if necessary. "Nutrient" means a substance in which a plant causes some growth or reproductive abnormality due to the deficiency of the substance, and the recovery of the symptom cannot be compensated by the supply of other substances. In principle, it means an essential element of plants. As essential elements of general plants, 17 kinds of elements, that is, hydrogen (H), oxygen (O), carbon (C), nitrogen (N), phosphorus (P), potassium (K), magnesium (Mg) ), Calcium (Ca), Sulfur (S), Iron (Fe), Manganese (Mg), Zinc (Zn), Boron (B), Molybdenum (Mo), Copper (Cu), Chlorine (Cl), and Nickel ( Ni) can be mentioned. In addition, fertilizers containing these elements (for example, urea, ammonium salts, (per) phosphate) are also included in the nutrients referred to here.
１−３−２．剤型
本発明の植物生育促進剤の剤型は、特に限定はしない。例えば、液体状態、又は固体状態とすることができる。液体状態の場合、各構成成分を適切な溶液に懸濁したものであればよい。適切な溶液としては、例えば、水（滅菌水、脱イオン水、超純水を含む）、バッファー、液体培地等が挙げられる。固体状態の場合、例えば、顆粒状態、粉末状態、又はゲルのような半固体状態が挙げられる。
1-3-2. Dosage Form The dosage form of the plant growth promoter of the present invention is not particularly limited. For example, it can be in a liquid state or a solid state. In the liquid state, each component may be suspended in an appropriate solution. Suitable solutions include, for example, water (including sterile water, deionized water, ultrapure water), buffers, liquid media and the like. In the case of a solid state, for example, a granular state, a powder state, or a semi-solid state such as a gel can be mentioned.
本発明の植物生育促進剤の剤型の具体例として、液剤、粉剤、粒剤、水和剤、育苗培土等の剤型が挙げられる。 Specific examples of the dosage form of the plant growth promoter of the present invention include dosage forms such as liquids, powders, granules, wettable powders, and seedling raising soil.
２．植物栽培方法
２−１．概要
本発明の第２の態様は、植物栽培方法である。本態様の植物栽培方法は、前記第１態様の植物生育促進剤を対象植物に施用して当該植物を栽培することを特徴とする。本態様の植物栽培方法により、対象植物の生育を促進させることができる。
2. Plant cultivation method 2-1. Outline The second aspect of the present invention is a plant cultivation method. The plant cultivation method of this embodiment is characterized in that the plant growth promoter of the first aspect is applied to a target plant to cultivate the plant. By the plant cultivation method of this embodiment, the growth of the target plant can be promoted.
２−２．方法
本発明の植物栽培方法は、必須の工程として施用工程及び栽培工程を含む。以下、それぞれの工程について説明する。
2-2. Method The plant cultivation method of the present invention includes an application step and a cultivation step as essential steps. Hereinafter, each step will be described.
２−２−１．施用工程
本明細書において「施用工程」は、前記第１態様の植物生育促進剤を対象植物に施用する工程である。施用工程では、植物生育促進剤の有効成分であるグルコン酸金属キレートとベタインを対象植物に付与することを目的とする。
2-2-1. Application step In the present specification, the "application step" is a step of applying the plant growth promoter of the first aspect to a target plant. The purpose of the application step is to impart metal gluconate chelate and betaine, which are the active ingredients of the plant growth promoter, to the target plant.
施用方法については、対象植物に第１態様の植物生育促進剤を施用することができる方法であれば、当該分野で公知の方法を用いればよく、特に限定はされない。第１態様の植物生育促進剤の有効成分は、対象植物の茎葉部、及び根部等、植物体表面全体から吸収され得ることから、栽培用途や目的に合わせて施用することができる。例えば、茎葉部等に直接、塗布、噴霧、散布又は浸漬によって接触させる施用法であってもよい。また後述する栽培工程において、対象植物を、土壌で栽培するのであれば土壌中に、培地であれば培地中に、また水耕栽培であれば水耕液中に、添加すればよい。ここでいう「土壌」は、対象植物の生育が可能な土壌であれば特に制限はしない。通常は、適当な栄養素を含み、適切なpH値を有する対象植物の栽植用土壌が利用される。土壌の場所は、問わない。播種又は苗の植え付け前の本圃土壌又は育苗土壌のいずれであってもよい。また、「培地」は、人工的に調製した対象植物の栽植用培地をいう。寒天培地のような固体培地であってもよいし、液体培地であってもよい。培地の例として、例えば、隔離ベッド、根域制限ポット又は苗床が挙げられる。培地の組成は、当該分野で公知の培地組成でよい。植物の種類等によって適宜選択することができる。「水耕液」とは、水耕栽培に用いる液体培地又は液体肥料をいう。水耕液の組成は、当該分野で公知の水耕液でよい。例えば、社団法人 日本施設園芸協会編、培養液栽培新マニュアル、に記載の組成に基づいて調製することができる。また、水耕液は、園芸用品メーカーからも市販されており、それらを利用してもよい。施用後は、植部生育促進剤を均一化するため、土壌、培地、水耕液などを十分に撹拌することが好ましい。 As for the application method, any method known in the art may be used as long as the plant growth promoter of the first aspect can be applied to the target plant, and the application method is not particularly limited. Since the active ingredient of the plant growth promoter of the first aspect can be absorbed from the entire surface of the plant such as the foliage and roots of the target plant, it can be applied according to the cultivation use and purpose. For example, it may be an application method in which the foliage portion or the like is directly contacted by application, spraying, spraying or dipping. Further, in the cultivation step described later, the target plant may be added to the soil if it is cultivated in soil, to the medium if it is a medium, or to the hydroponic solution if it is hydroponics. The "soil" referred to here is not particularly limited as long as it is a soil in which the target plant can grow. Usually, the planting soil of the target plant containing appropriate nutrients and having an appropriate pH value is used. The location of the soil does not matter. It may be either field soil or seedling raising soil before sowing or planting of seedlings. In addition, "medium" refers to an artificially prepared medium for planting a target plant. It may be a solid medium such as an agar medium or a liquid medium. Examples of media include isolation beds, root zone limiting pots or nurseries. The composition of the medium may be a medium composition known in the art. It can be appropriately selected depending on the type of plant and the like. "Hydroponic liquid" refers to a liquid medium or liquid fertilizer used for hydroponics. The composition of the hydroponic solution may be a hydroponic solution known in the art. For example, it can be prepared based on the composition described in the New Manual for Cultivation of Culture Solution, edited by the Japan Facility Gardening Association. Further, the hydroponic liquid is commercially available from a gardening equipment manufacturer, and they may be used. After application, it is preferable to sufficiently stir the soil, medium, hydroponic solution, etc. in order to homogenize the plant growth promoter.
２−２−２．栽培工程
「栽培工程」は、前記施用工程後の対象植物を所定の期間栽培する工程である。本工程は、第１態様の植物生育促進剤を付与した対象植物を所望の状態にまで生育させることを目的とする。
2-2-2. Cultivation step The "cultivation step" is a step of cultivating the target plant after the application step for a predetermined period. The purpose of this step is to grow the target plant to which the plant growth promoter of the first aspect has been applied to a desired state.
栽培工程における、植物の栽培形態は、特に制限はしない。土壌栽培、培地栽培、水耕栽培のいずれの栽培形態も適用できる。対象植物の種類や栽培目的に合わせて適宜選択すればよい。各栽培形態の具体的な方法については、公知の方法に基づいて実施すればよい。栽培における、明暗時間（光照射時間及び暗黒時間）、気温及び湿度等の気象条件、二酸化炭素（CO2）濃度、生育期間、及び肥料や至適pH等の栽培条件については、対象植物に合わせて適宜選択する。 The cultivation form of the plant in the cultivation process is not particularly limited. Any cultivation form of soil cultivation, medium cultivation, and hydroponics can be applied. It may be appropriately selected according to the type of the target plant and the purpose of cultivation. The specific method of each cultivation form may be carried out based on a known method. In cultivation, the light and dark time (light irradiation time and dark time), meteorological conditions such as temperature and humidity, carbon dioxide (CO 2 ) concentration, growing period, and cultivation conditions such as fertilizer and optimum pH are adjusted according to the target plant. And select as appropriate.
２−３．効果
本態様の植物栽培方法によれば、第１態様の植物生育促進剤を用いて植物を栽培することで、該植物生育促進剤を使用しない栽培法と比較して、同じ栽培期間内で植物バイオマス生産量を増加することができる。
2-3. Effect According to the plant cultivation method of this embodiment, by cultivating a plant using the plant growth promoter of the first embodiment, the plant is cultivated within the same cultivation period as compared with the cultivation method without the plant growth promoter. Biomass production can be increased.
＜実施例１：植物生育促進剤による植物の生育促進効果の検証（１）＞
（目的）
本発明の植物生育促進剤の有効成分による植物の生育促進効果について検証する。
<Example 1: Verification of plant growth promoting effect by plant growth promoting agent (1)>
(Purpose)
We will verify the plant growth promoting effect of the active ingredient of the plant growth promoting agent of the present invention.
（材料と方法）
（１）供試植物にはトマト（品種：レジナ）を用いた。
（２）供試薬剤には、グルコン酸銅、グルコン酸亜鉛、及びベタインを超純水に溶解して使用した。
（３）供試試験区として6試験区を設置した。各試験区の詳細は、（ａ）対照区：供試薬剤無付与（水散布）、（ｂ）ベタイン区：ベタインのみを終濃度100ppmで付与、（ｃ）グルコン酸亜鉛区：グルコン酸亜鉛のみを終濃度0.5mMで付与、（ｄ）グルコン酸銅区：グルコン酸銅のみを終濃度0.5mMで付与、（ｅ）グルコン酸亜鉛／ベタイン区：終濃度0.5mMグルコン酸亜鉛と終濃度100ppmのベタインを付与、（ｆ）グルコン酸銅／ベタイン区：終濃度0.5mMグルコン酸銅と終濃度100ppmのベタインを付与、である。
（４）栽培条件は、室温25℃、明条件16時間、暗条件8時間に設定した人工気象室内で土壌栽培した。
（５）薬剤の付与方法は、播種2週間後の本葉1.5〜1.8葉期から各濃度に調整した水溶液を供試植物の茎葉に散布して行った。散布は、播種2週間後から開始し、以降6〜8日毎に合計3回行った。
（６）生育調査は、最終散布から10日後に地上部新鮮重、及び地下部新鮮重を測定した。
(Materials and methods)
(1) Tomato (variety: Regina) was used as the test plant.
(2) Copper gluconate, zinc gluconate, and betaine were dissolved in ultrapure water and used as a reagent agent.
(3) Six test plots were set up as test plots. The details of each test group are as follows: (a) Control group: No reagent agent added (water spraying), (b) Betain group: Only betaine is given at a final concentration of 100 ppm, (c) Zinc gluconate group: Zinc gluconate only (D) Copper gluconate group: Only copper gluconate is given at a final concentration of 0.5 mM, (e) Zinc gluconate / betaine group: Final concentration 0.5 mM Zinc gluconate and final concentration 100 ppm Add betaine, (f) Copper gluconate / betaine group: Grant a final concentration of 0.5 mM copper gluconate and a final concentration of 100 ppm of betaine.
(4) Soil cultivation was carried out in an artificial weather room set at room temperature of 25 ° C., bright conditions for 16 hours, and dark conditions for 8 hours.
(5) As a method of applying the drug, an aqueous solution adjusted to each concentration was sprayed on the stems and leaves of the test plant from the 1.5 to 1.8 leaf stage of the true leaves 2 weeks after sowing. Spraying started 2 weeks after sowing and was performed 3 times every 6 to 8 days thereafter.
(6) In the growth survey, the fresh weight of the above-ground part and the fresh weight of the underground part were measured 10 days after the final spraying.
（結果）
図１及び図２に結果を示す。図１は、（ａ）対照区、（ｂ）ベタイン区、（ｃ）グルコン酸亜鉛区、及び（ｅ）グルコン酸亜鉛／ベタイン区を示す図であり、図２は、（ａ）対照区、（ｂ）ベタイン区、（ｄ）グルコン酸銅区、及び（ｆ）グルコン酸銅／ベタイン区を示す図である。図１及び図２、共にAは植物地上部の新鮮重を、またBは植物地下部の新鮮重（根重）を示す。
(result)
The results are shown in FIGS. 1 and 2. FIG. 1 is a diagram showing (a) control group, (b) betaine group, (c) zinc gluconate group, and (e) zinc gluconate / betaine group, and FIG. 2 is a diagram showing (a) control group, It is a figure which shows (b) betaine group, (d) copper gluconate group, and (f) copper gluconate / betaine group. In both FIGS. 1 and 2, A indicates the fresh weight of the above-ground part of the plant, and B indicates the fresh weight (root weight) of the underground part of the plant.
図１において、（ｂ）ベタイン区では、従来の結果と同様、地下部の新鮮重のみが（ａ）対照区よりも高くなる傾向が見られた。一方、（ｃ）グルコン酸亜鉛区では、地上部及び地下部共に（ａ）対照区よりもやや高くなる傾向が見られた。ところが、（ｅ）グルコン酸亜鉛／ベタイン区では、（ａ）対照区よりも顕著に高くなり、また（ｂ）ベタイン区及び（ｃ）グルコン酸亜鉛区よりも、地上部及び地下部共に明らかに高くなった。 In FIG. 1, in the (b) betaine plot, only the fresh weight of the underground part tended to be higher than that in the (a) control plot, as in the conventional results. On the other hand, in the (c) zinc gluconate group, both the above-ground part and the underground part tended to be slightly higher than the (a) control group. However, in the (e) zinc gluconate / betaine group, it was significantly higher than in the (a) control group, and it was clearly higher in both the above-ground part and the underground part than in the (b) betaine group and (c) zinc gluconate group. It got higher.
図２でも図１とほぼ同様の結果が得られた。（ｂ）ベタイン区では、従来の結果と同様、地下部の新鮮重のみが（ａ）対照区よりも高くなる傾向が見られた。一方、（ｄ）グルコン酸銅区では、地上部は（ａ）対照区よりもやや高くなったが、逆に地下部で（ａ）対照区より低い結果となった。ところが、（ｆ）グルコン酸銅／ベタイン区では、（ａ）対照区よりも顕著に高くなり、また（ｂ）ベタイン区及び（ｄ）グルコン酸銅区よりも、地上部及び地下部共に明らかに高くなった。 In FIG. 2, almost the same result as in FIG. 1 was obtained. In the (b) betaine plot, only the fresh weight of the underground part tended to be higher than that in the (a) control plot, as in the conventional results. On the other hand, in the (d) copper gluconate group, the above-ground part was slightly higher than the (a) control group, but conversely, the underground part was lower than the (a) control group. However, in the (f) copper gluconate / betaine section, it was significantly higher than in the (a) control section, and it was clearly higher in both the above-ground part and the underground part than in the (b) betaine section and (d) copper gluconate section. It got higher.
以上のことから、グルコン酸金属キレートであるグルコン酸亜鉛又はグルコン酸銅とベタインの組み合わせによって、相乗的な生育促進効果が現れることが立証された。 From the above, it was proved that a synergistic growth promoting effect appears by the combination of zinc gluconate or copper gluconate, which is a metal gluconate chelate, and betaine.
＜実施例２：植物生育促進剤による植物の生育促進効果の検証（２）＞
（目的）
イチゴの繁殖には種子繁殖と栄養繁殖がある。栄養繁殖は親株の遺伝形質を受継ぎ、比較的早く生育させることが可能なため、イチゴでは主に栄養繁殖が行われている。栄養繁殖は、ランナー（匍匐枝）と呼ばれる蔓のように長く伸びる茎の節にできる新芽によって行われる。この新芽を土に触れさせておくと、新芽から根が伸びて、葉が成長し、切り離せば新たな苗（ランナー子株）になる。苗の採取方法には大きく2種類あり、新芽の葉が2〜3葉位まで育った段階で親株から完全に切り離して別の鉢に植え付ける「挿し苗法」と、親株から切り離さず新芽の部分の下に鉢をおき、ピンで新芽を鉢に固定して、十分に大きくなった後に切り離す方法の2種類がある。「挿し苗法」は、親株のランナーから切り離して栽培するので場所の制限はないが、切り離し後に根が伸長していない場合、枯れてしまう。そこで、本発明の植物生育促進剤が挿し苗法における発根促進とその伸長において、ベタイン以上に有効であることを検証する。
<Example 2: Verification of plant growth promoting effect by plant growth promoting agent (2)>
(Purpose)
Strawberry propagation includes seed propagation and vegetative propagation. Since vegetative propagation inherits the genetic traits of the parent strain and can grow relatively quickly, vegetative propagation is mainly carried out in strawberries. Vegetative propagation is carried out by sprouts called runners (stolons) that form on the nodes of long stems that grow like vines. When this sprout is exposed to the soil, the root grows from the sprout, the leaves grow, and when separated, it becomes a new seedling (runner seedling). There are two main methods for collecting seedlings: the "cutting seedling method" in which the shoots are completely separated from the parent strain and planted in another pot when the shoots have grown to about 2 to 3 leaves, and the shoots that are not separated from the parent strain. There are two methods: place the pot underneath, fix the sprout to the pot with a pin, and cut it off after it is large enough. In the "cutting seedling method", the roots are cultivated separately from the runner of the parent strain, so there are no restrictions on the location, but if the roots do not grow after cutting, they will die. Therefore, it is verified that the plant growth promoter of the present invention is more effective than betaine in promoting rooting and its elongation in the seedling method.
（材料と方法）
（１）供試植物にはイチゴ（品種：女峰）を用いた。
（２）供試薬剤には、グルコン酸亜鉛、及びベタインを超純水に溶解して使用した。
（３）供試試験区として4試験区を設置した。各試験区の詳細は、（ａ’）対照区：供試薬剤無付与（水散布）、（ｂ’）ベタイン区：ベタインのみを終濃度100ppmで付与、（ｃ’）グルコン酸亜鉛区：グルコン酸亜鉛のみを終濃度0.5mMで付与、（ｄ’）グルコン酸亜鉛／ベタイン区：終濃度0.5mMグルコン酸亜鉛と終濃度100ppmのベタインを付与、である。
（４）栽培条件は、ガラスハウス内で土壌栽培にて行った。
（５）採苗は、ランナー子株が本葉2葉になった時に第2節間の途中で切り取り採苗した。なお、採苗時点での発根は見られなかった。
（６）薬剤の付与方法は、ランナー子株を切り離す9日前から散布を開始、各濃度に調整した水溶液を子株だけでなく、親株の茎葉にまんべんなく散布した。挿し苗後も同様に子株の茎葉に散布した。散布頻度は、ランナー子株を切り離す9日前及び1日前、及び採苗後は6日毎に2回、合計4回散布した。
（７）生育調査は、最終散布から15日後に地下部新鮮重（新根重）を測定した。
(Materials and methods)
(1) Strawberries (variety: female peak) were used as the test plant.
(2) Zinc gluconate and betaine were dissolved in ultrapure water and used as a reagent agent.
(3) Four test plots were set up as test plots. The details of each test group are as follows: (a') Control group: No reagent agent added (water spraying), (b') Betaine group: Only betaine was given at a final concentration of 100 ppm, (c') Zinc gluconate group: Glucon Only zinc acid acid was given at a final concentration of 0.5 mM, and (d') zinc gluconate / betaine group: zinc gluconate at a final concentration of 0.5 mM and betaine at a final concentration of 100 ppm were given.
(4) Cultivation conditions were soil cultivation in a glass house.
(5) Seedlings were collected by cutting in the middle of the second node when the runner cotyledon became two true leaves. No rooting was observed at the time of seedling collection.
(6) As a method of applying the drug, spraying was started 9 days before the runner seedlings were separated, and the aqueous solution adjusted to each concentration was sprayed evenly not only on the seedlings but also on the foliage of the parent strain. After cutting, the seedlings were similarly sprayed on the foliage of the offspring. The frequency of spraying was 9 days and 1 day before cutting off the runner seedlings, and twice every 6 days after seedling collection, for a total of 4 sprays.
(7) In the growth survey, the fresh weight (new root weight) in the underground was measured 15 days after the final spraying.
（結果）
図３に結果を示す。（ａ’）は対照区、（ｂ’）はベタイン区、（ｃ’）はグルコン酸亜鉛区、及び（ｄ’）はグルコン酸亜鉛／ベタイン区で、いずれも植物地下部の新鮮重（新根重）を示す図である。
(result)
The results are shown in FIG. (A') is a control group, (b') is a betaine group, (c') is a zinc gluconate group, and (d') is a zinc gluconate / betaine group, all of which are fresh weights (new) in the underground part of the plant. It is a figure which shows the root weight).
図３から、ベタイン区（ｂ’）及びグルコン酸亜鉛区（ｃ’）の新根重は、対照区（ａ’）のそれと有意な差はなく、ほぼ同等で、新根発生効果は明らかではなかった。ところが、（ｄ’）グルコン酸亜鉛／ベタイン区では、（ａ）対照区や（ｂ）ベタイン区及び（ｃ’）グルコン酸亜鉛区よりも新根重は顕著に高く、グルコン酸亜鉛とベタインの組み合わせによって、相乗的な新根の発根促進効果を有することが明らかとなった。 From FIG. 3, the new root weights of the betaine group (b') and the zinc gluconate group (c') are not significantly different from those of the control group (a') and are almost the same, and the new root development effect is clear. There wasn't. However, in the (d') zinc gluconate / betaine group, the new root weight was significantly higher than in the (a) control group, (b) betaine group and (c') zinc gluconate group, and the zinc gluconate and betaine were significantly higher. It was clarified that the combination had a synergistic effect of promoting the rooting of new roots.
Claims (7)
前記無機金属塩が無機銅、無機亜鉛、及び無機鉄からなる群から選択される１以上の塩である、請求項３に記載の植物生育促進剤。 The gluconate is one or more salts selected from the group consisting of sodium gluconate, potassium gluconate, and calcium gluconate.
The plant growth promoter according to claim 3, wherein the inorganic metal salt is one or more salts selected from the group consisting of inorganic copper, inorganic zinc, and inorganic iron.
請求項１〜６のいずれか１項に記載の植物生育促進剤を対象植物に施用する施用工程、及び
該対象植物を栽培する栽培工程
を含む前記方法。 It ’s a plant cultivation method.
The method comprising an application step of applying the plant growth promoter according to any one of claims 1 to 6 to a target plant, and a cultivation step of cultivating the target plant.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20230067206A (en) * | 2021-11-09 | 2023-05-16 | 오미경 | Plant fertilization promoter that promote pollination of self-incompatibility fruit |
| KR102613157B1 (en) * | 2021-11-09 | 2023-12-12 | 오미경 | Plant fertilization promoter that promote pollination of self-incompatibility fruit |
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