JP2021164409A - Production method of low-fat soy milk lactic acid fermented product - Google Patents
Production method of low-fat soy milk lactic acid fermented product Download PDFInfo
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 title claims abstract description 158
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
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- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
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- YBHQCJILTOVLHD-YVMONPNESA-N Mirin Chemical compound S1C(N)=NC(=O)\C1=C\C1=CC=C(O)C=C1 YBHQCJILTOVLHD-YVMONPNESA-N 0.000 description 1
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Abstract
Description
本発明は、低脂肪豆乳乳酸発酵物の製造方法に関する。 The present invention relates to a method for producing a low-fat soymilk lactic acid fermented product.
「食資源不足」や「環境」といった問題に対する、社会的な危機感が、ますます高まっており、持続可能な世界を目指す取組が進んでいる。
とりわけ、世界的な人口増加により、食料(特に動物性たんぱく源)および水資源が将来不足すると予測されている。
この、人口増加と共に起こる「食資源不足」や「環境」といった社会課題に対し、植物性の食の素材を通じた解決が試みられている。
There is an increasing sense of social crisis regarding issues such as "food resource depletion" and "environment", and efforts are underway to aim for a sustainable world.
In particular, global population growth is projected to lead to future shortages of food (especially animal protein sources) and water resources.
Attempts are being made to solve these social issues such as "food resource depletion" and "environment" that occur with population growth through plant-based food materials.
特に、この人類の社会課題に対し、大豆は有効なソリューションの一つであると考えられる。
なぜならば、大豆は寒冷地から熱帯まで幅広い地域において、動物性のたんぱく源に比べ、わずかな肥料、水で、大量に栽培することが可能だからである。
In particular, soybeans are considered to be one of the effective solutions to this social issue of humankind.
This is because soybeans can be cultivated in large quantities in a wide range of areas from cold regions to the tropics with a small amount of fertilizer and water compared to animal protein sources.
従来、大豆は、搾油により得られる大豆油や、大豆を絞って得られる豆乳、更に脱脂大豆から得られる粉末状大豆たんぱくや粒状大豆たんぱく等、様々に加工されて、幅広い用途に利用されている。
一方で、大豆たんぱく質はコレステロールを含まない良質なたんぱく質であり、地球環境負荷は低いものの、青草味、えぐ味などの特有の風味を有するため、その利用が制限されている面もある。
Conventionally, soybeans have been variously processed and used for a wide range of purposes, such as soybean oil obtained by squeezing oil, soymilk obtained by squeezing soybeans, and powdered soybean protein and granular soybean protein obtained from defatted soybeans. ..
On the other hand, soybean protein is a high-quality protein that does not contain cholesterol, and although it has a low impact on the global environment, it has unique flavors such as green grass taste and harsh taste, so its use is restricted in some respects.
また、稲から収穫される米を原料とし、麹による糖化・酵母によるアルコール発酵を経て得られたもろみを圧搾することで、「清酒」と、分離した搾りかすとして「酒粕」が得られる。
「酒粕」中には、米、米麹、酵母に由来した成分である各種アミノ酸類、ビタミン類、有機酸類、タンパク質、各種糖類などの多くの栄養成分、有効成分が含まれている。
しかしながら、酒粕は甘酒、漬物、粕汁、あるいはそのまま食料とする以外では、飼料、肥料として処理されており、食品としての需要・流通が低迷している。
In addition, by using rice harvested from rice as a raw material and squeezing the mash obtained through saccharification with jiuqu and alcoholic fermentation with yeast, "sake" and "sake lees" can be obtained as separated pomace.
"Sake lees" contains many nutritional components and active ingredients such as various amino acids, vitamins, organic acids, proteins, and various sugars, which are components derived from rice, rice jiuqu, and yeast.
However, sake lees are processed as feed and fertilizer other than amazake, pickles, lees juice, or as food as they are, and the demand and distribution as food is sluggish.
一方、酒粕の有効利用を目的に、例えば、特許文献1では、酒粕を原料とした新規な酒粕発酵エキスが、特許文献2では、液化酒粕をデンプン分解酵素処理およびタンパク分解酵素処理と平行して、酵母で発酵させ、次いで、固液分離を行って固体成分を除去することにより得られる酒粕発酵エキスを用いた風味改良剤が、特許文献3では、酒粕由来の成分を有効成分として含有する乳化剤、および脂溶性物質を含む、油脂含有水溶性組成物が、特許文献4では、酒粕由来の成分を有効成分として含有することを特徴とする乳化剤が提案されているが、用途が限定されている。
また、酒粕には100gあたり5.2gと、白米の10倍もの食物繊維が含まれており、ざらつきや、口残りといった食感の点で、食品用途としての利用が敬遠される傾向があった。
On the other hand, for the purpose of effective utilization of liquor lees, for example, in Patent Document 1, a novel liquor lees fermented extract made from liquor lees is used as a raw material, and in Patent Document 2, liquefied liquor lees are treated in parallel with starch-degrading enzyme treatment and proteolytic enzyme treatment. , A flavor improver using a liquor starch fermented extract obtained by fermenting with yeast and then performing solid-liquid separation to remove solid components. In Patent Document 4, an emulsifier containing an oil-and-fat-containing water-soluble composition containing a fat-soluble substance and a fat-soluble substance containing a component derived from liquor starch as an active ingredient has been proposed, but its use is limited. ..
In addition, sake lees contain 5.2 g per 100 g, which is 10 times as much dietary fiber as white rice, and there is a tendency to avoid using it for food purposes in terms of texture such as roughness and mouth residue. ..
本発明の課題は、植物性の食の素材である、「大豆」と「酒粕」を有効利用し、食品用途としての新たな素材を提供することで、「食資源不足」や「環境」といった社会課題の解決に貢献することである。 The subject of the present invention is to effectively utilize "soybeans" and "liquor lees", which are plant-based food materials, and to provide new materials for food use, such as "food resource depletion" and "environment". It is to contribute to the solution of social issues.
本発明者は上記課題に対して鋭意研究を行った結果、低脂肪豆乳に酒粕を添加し、乳酸発酵後、加圧均質化処理、又は低脂肪豆乳に酒粕を添加し、加圧均質化処理後、乳酸発酵、あるいは乳酸発酵した低脂肪豆乳に、酒粕を添加し、加圧均質化処理することで、大豆の風味が改善され、動物性の食の素材には無い、コク・うまみを有し、かつ、ざらつきや、口残りが無い、なめらかで口溶けの良い低脂肪豆乳乳酸発酵物が得られることを見出し、本発明を完成させるに至った。 As a result of diligent research on the above-mentioned problems, the present inventor added sake lees to low-fat soymilk and subjected to pressure homogenization treatment after lactic acid fermentation, or pressure homogenization treatment by adding sake lees to low-fat soymilk. After that, by adding liquor lees to lactic acid-fermented or lactic-fermented low-fat soymilk and subjecting it to pressure homogenization, the flavor of soybeans is improved, and it has richness and taste that are not found in animal food ingredients. However, they have found that a low-fat soymilk lactic acid fermented product that is smooth and has good melting in the mouth without roughness or residue in the mouth can be obtained, and has completed the present invention.
すなわち本発明は、下記の発明を包含するものである。
(1)脂質含量2.5重量%以下の低脂肪豆乳に酒粕を添加し、乳酸発酵することを特徴とする低脂肪豆乳乳酸発酵物の製造方法。
(2)乳酸発酵の前、及び/又は乳酸発酵の後に加圧均質化処理を行う請求項1記載の低脂肪豆乳乳酸発酵物の製造方法。
(3)乳酸発酵した、脂質含量2.5重量%以下の低脂肪豆乳に、酒粕を添加し、加圧均質化処理することを特徴とする低脂肪豆乳乳酸発酵物の製造方法。
(4)(1)〜(3)いずれかに記載の低脂肪豆乳乳酸発酵物を使用することを特徴とする飲料および食品。
That is, the present invention includes the following inventions.
(1) A method for producing a low-fat soymilk lactic acid fermented product, which comprises adding sake lees to low-fat soymilk having a lipid content of 2.5% by weight or less and lactic acid fermentation.
(2) The method for producing a low-fat soymilk lactic acid fermented product according to claim 1, wherein the pressure homogenization treatment is performed before and / or after the lactic acid fermentation.
(3) A method for producing a low-fat soymilk lactic acid fermented product, which comprises adding sake lees to lactic acid-fermented low-fat soymilk having a lipid content of 2.5% by weight or less and subjecting it to pressure homogenization treatment.
(4) Beverages and foods comprising the low-fat soymilk lactic acid fermented product according to any one of (1) to (3).
本発明によって、大豆の風味が改善され、動物性の食の素材には無い、コク・うまみを有し、かつ、ざらつきや、口残りが無い、なめらかで口溶けの良い低脂肪豆乳乳酸発酵物および、これを使用した飲料および食品を提供することができ、「大豆」と「酒粕」の有効利用さらには、「食資源不足」や「環境」の社会課題に対し、植物性の食の素材が有効なソリューションの一つとなり得る。 According to the present invention, the flavor of soybean is improved, and a low-fat soymilk lactic acid fermented product having richness and taste, which is not found in animal food materials, and has no roughness or residue, and is smooth and melts in the mouth. , Beverages and foods using this can be provided, and effective use of "soybeans" and "sake lees". Furthermore, in response to social issues such as "insufficient food resources" and "environment", plant-based food ingredients It can be one of the effective solutions.
以下、本発明を詳細に説明する。 Hereinafter, the present invention will be described in detail.
(低脂肪豆乳)
本発明の低脂肪豆乳乳酸発酵物の製造方法において、低脂肪豆乳は、脂質含量が低減された豆乳をいう。
本発明で使用する低脂肪豆乳は、強調表示に則り脂質含量2.5重量%以下、好ましくは2重量%、より好ましくは1.5重量%以下に低減された低脂肪豆乳を用いる。
低脂肪豆乳は、牛乳における低脂肪乳に相当する特長を持った大豆の素材である。
豆乳の低カロリー化の実現は勿論、大豆脂質による風味劣化の低減を可能にしている。
低脂肪豆乳の脂質含量は、該低脂肪豆乳の蛋白質含量に対して、好ましくは40重量%以下であり、より好ましくは20重量%以下であり、更に好ましくは10重量%以下である。また、本発明の低脂肪豆乳とは、丸大豆や脱脂大豆から水で抽出し、不溶性繊維であるオカラを除去して得られる、豆乳だけでなく、豆乳からオカラを除去せずにオカラを微粉砕したスラリー状のものも含まれ、好ましくはオカラを除去した豆乳が好ましい。
(Low-fat soymilk)
In the method for producing a low-fat soymilk lactic acid fermented product of the present invention, low-fat soymilk refers to soymilk having a reduced lipid content.
As the low-fat soymilk used in the present invention, low-fat soymilk having a lipid content of 2.5% by weight or less, preferably 2% by weight, more preferably 1.5% by weight or less is used according to the highlighting.
Low-fat soymilk is a soybean material having characteristics equivalent to low-fat milk in milk.
Not only is it possible to reduce the calories of soymilk, but it is also possible to reduce the deterioration of flavor caused by soybean fat.
The lipid content of the low-fat soymilk is preferably 40% by weight or less, more preferably 20% by weight or less, still more preferably 10% by weight or less, based on the protein content of the low-fat soymilk. Further, the low-fat soymilk of the present invention is obtained by extracting okara, which is an insoluble fiber, from whole soybeans or defatted soybeans with water, and not only soymilk, but also fine okara without removing okara from soymilk. A crushed slurry is also included, and soymilk from which okara has been removed is preferable.
(低脂肪豆乳の脂質含量の測定方法)
サンプル(W)をクロロホルム:メタノールが2:1(体積比)の混合溶媒を用い、常圧沸点において30分間抽出された抽出物を無水硫酸ナトリウムで脱水後石油エーテルへ転溶する。
これを遠心分離しエーテル層を重量既知のフラスコ(W1)に採取し、エーテルを留去後、乾燥重量(エーテル留去後のフラスコの重量)(W2)を測定する。
W、W1、W2、およびサンプル(W)の乾物重量(W3)の測定値を用い、以下の式により得られる値を脂質含量(%/dry)とする。
脂質含量(%/dry)=[{(W2−W1)/W×2.5×100}/W3×100(%)]
溶媒抽出装置としてはFOSS社製の「ソックステック」を用いることができる。
上記の測定法は「クロロホルム/メタノール混合溶媒抽出法」と称するものとする。
(Measuring method of lipid content of low-fat soymilk)
The sample (W) is dehydrated with anhydrous sodium sulfate using a mixed solvent of chloroform: methanol in a ratio of 2: 1 (volume ratio) for 30 minutes at an atmospheric boiling point, and then transferred to petroleum ether.
This is centrifuged, and the ether layer is collected in a flask (W1) having a known weight. After distilling off the ether, the dry weight (weight of the flask after distilling off the ether) (W2) is measured.
Using the measured values of the dry weight (W3) of W, W1, W2, and the sample (W), the value obtained by the following formula is defined as the lipid content (% / dry).
Lipid content (% / dry) = [{(W2-W1) / W × 2.5 × 100} / W3 × 100 (%)]
As the solvent extraction device, "Socks Tech" manufactured by FOSS can be used.
The above measurement method shall be referred to as "chloroform / methanol mixed solvent extraction method".
(低脂肪豆乳の製造方法)
低脂肪豆乳の大豆原料は、丸大豆、半割れ大豆、グリッツ、粉末等の形態のものが挙げられる。
また、大豆原料として、あらかじめ脱脂や減脂されたものを好ましく用いることもできる。
低脂肪豆乳は、公知の製造法を用いて調製することができ、例えば、ヘキサンやエタノール等の溶剤により脱脂して得られた脱脂大豆から水抽出する方法を好ましく使用できる。
水抽出は、一般的な方法を用いることができ、例えば水性媒体を原料大豆に加えて攪拌・磨砕してスラリー状とし、不溶性画分(オカラ)を遠心分離、濾過等により分離し、除去する方法が例示される。
また、丸大豆や減脂大豆を水抽出後、スラリー状態或はオカラの除去後に溶剤抽出や膜処理、又は遠心分離による脱脂操作を行うことによって低脂肪豆乳を得る方法でもよい。
粉末化された低脂肪豆乳を水に溶解して用いることもできる。
水抽出は、大豆原料に対して、好ましくは3重量倍〜20重量倍、より好ましくは4重量倍〜15重量倍程度の加水をする。
加水倍率は高い方が水溶性成分の抽出率が高まり、分離を良くすることができるが、高過ぎると濃縮が必要となりコストがかかる。
また、抽出処理を2回以上繰り返すと水溶性成分の抽出率をより高めることができる。
抽出温度は、特に限定されない。効率良く蛋白質を抽出するには5℃〜98℃で行うことが好ましい。
また、丸大豆を水抽出後、スラリー状態或はオカラの除去後において溶剤抽出や膜処理による脱脂操作を行うことによって低脂肪豆乳を得ることも可能である。
また、特開2012−016348号公報に開示される方法等も利用できる。
即ち、乾物当たりの脂質含量が15重量%以上、NSI(Nitrogen Solubility Index:窒素溶解指数)が20〜77の含脂大豆に加水して懸濁液を得、固液分離し、中性脂質および極性脂質を不溶性画分に移行させて除去し、蛋白質および糖質を含む水溶性画分を回収する。
この製法で得られる低脂肪豆乳は、乾物当たりの蛋白質および炭水化物の総含量が80重量%以上であり、脂質含量(クロロホルム/メタノール混合溶媒抽出物量としての含量)が蛋白質含量に対して10重量%未満であるという特徴を有する。
なお、ここでの蛋白質含量とは、ケルダール法により測定した窒素量に6.25の窒素換算係数を乗じた数値を指す。
また、脂質含量の低い豆乳に、脂質含量の高い豆乳や大豆油をブレンドし、脂質含量を調整してもよい。
(Manufacturing method of low-fat soymilk)
Examples of the soybean raw material of low-fat soymilk include whole soybeans, half-split soybeans, grits, powder and the like.
Further, as the soybean raw material, those which have been degreased or degreased in advance can be preferably used.
Low-fat soymilk can be prepared using a known production method. For example, a method of water extraction from defatted soybeans obtained by degreasing with a solvent such as hexane or ethanol can be preferably used.
For water extraction, a general method can be used. For example, an aqueous medium is added to raw soybeans, stirred and ground to form a slurry, and the insoluble fraction (okara) is separated by centrifugation, filtration, etc. and removed. The method of doing so is illustrated.
Alternatively, low-fat soymilk may be obtained by extracting whole soybeans or reduced-fat soybeans with water, removing the slurry state or okara, and then performing a solvent extraction, a film treatment, or a degreasing operation by centrifugation.
Powdered low-fat soymilk can also be used by dissolving it in water.
In water extraction, water is preferably added to the soybean raw material in an amount of 3 to 20 times by weight, more preferably 4 to 15 times by weight.
The higher the water content, the higher the extraction rate of water-soluble components and the better the separation, but if it is too high, concentration is required and cost is high.
Further, if the extraction process is repeated twice or more, the extraction rate of the water-soluble component can be further increased.
The extraction temperature is not particularly limited. In order to extract the protein efficiently, it is preferably performed at 5 ° C to 98 ° C.
It is also possible to obtain low-fat soymilk by performing a degreasing operation by solvent extraction or membrane treatment after extracting whole soybeans with water and then in a slurry state or after removing okara.
Further, the method disclosed in Japanese Patent Application Laid-Open No. 2012-016348 can also be used.
That is, a suspension was obtained by adding water to a fat-containing soybean having a lipid content of 15% by weight or more per dry matter and an NSI (Nitrogen Solubility Index) of 20 to 77, and solid-liquid separation was performed to obtain neutral lipids and neutral lipids. Polar lipids are transferred to the insoluble fraction and removed, and the water-soluble fraction containing proteins and sugars is recovered.
The low-fat soymilk obtained by this production method has a total protein and carbohydrate content of 80% by weight or more per dry matter, and a lipid content (content as a amount of chloroform / methanol mixed solvent extract) of 10% by weight with respect to the protein content. It has the characteristic of being less than.
The protein content here refers to a value obtained by multiplying the amount of nitrogen measured by the Kjeldahl method by a nitrogen conversion coefficient of 6.25.
Further, soymilk having a low lipid content may be blended with soymilk or soybean oil having a high lipid content to adjust the lipid content.
本発明の低脂肪豆乳乳酸発酵物の製造方法は、低脂肪豆乳又は乳酸発酵した低脂肪豆乳に酒粕を添加することを特徴とする。
低脂肪豆乳又は乳酸発酵した低脂肪豆乳に酒粕を添加することにより、動物性の食の素材には無い、コク・うまみを有する低脂肪豆乳乳酸発酵物を得ることができる。
本発明において酒粕とは、酒の製造過程において、原料の米や麹等の発酵物(もろみ)を圧搾した後に残る固形物であり、清酒を製造する際に副生する清酒粕が代表的であるが、清酒粕以外の酒粕としては、みりん粕、蒸留酒粕(焼酎粕等)が挙げられる。
酒粕は、例えば、液状、ペースト状、あるいは乾燥させた固形状、や粉砕した粉末状のものを用いることができる。
本発明においては、低脂肪豆乳又は乳酸発酵した低脂肪豆乳に、酒粕が固形物換算で1〜30重量%含まれることが好ましい。
The method for producing a low-fat soymilk lactic acid fermented product of the present invention is characterized by adding sake lees to low-fat soymilk or lactic-fermented low-fat soymilk.
By adding sake lees to low-fat soymilk or lactic acid-fermented low-fat soymilk, a low-fat soymilk lactic acid fermented product having richness and taste, which is not found in animal food materials, can be obtained.
In the present invention, sake lees is a solid substance remaining after pressing fermented products (moromi) such as rice and jiuqu, which are raw materials in the process of producing sake, and sake lees produced as a by-product in the production of sake is typical. However, examples of sake lees other than sake lees include mirin lees and distilled sake lees (such as shochu lees).
As the sake lees, for example, liquid, paste, dried solid, or crushed powder can be used.
In the present invention, it is preferable that the low-fat soymilk or the low-fat soymilk fermented with lactic acid contains 1 to 30% by weight of sake lees in terms of solid matter.
本発明の低脂肪豆乳乳酸発酵物の製造方法においては、低脂肪豆乳又は酒粕を添加した低脂肪豆乳を原料とし、乳酸菌等を加えて乳酸発酵行うことを特徴とする。
低脂肪豆乳あるいは酒粕を添加した低脂肪豆乳のいずれの原料でもよいが、酒粕を添加した低脂肪豆乳を乳酸発酵することで、低脂肪豆乳由来の大豆特有の好ましくない風味が改善されるだけではく、酒粕由来の旨味・コクといった好ましい風味を付与しながらも、さらに乳酸発酵により、風味のカドがとれ、より美味しい低脂肪豆乳乳酸発酵物を得ることができる。
The method for producing a low-fat soymilk lactic acid fermented product of the present invention is characterized in that low-fat soymilk or low-fat soymilk to which sake lees are added is used as a raw material, and lactic acid fermentation is carried out by adding lactic acid bacteria and the like.
Either low-fat soymilk or low-fat soymilk with liquor lees can be used as a raw material, but lactic acid fermentation of low-fat soymilk with liquor lees only improves the unfavorable flavor peculiar to soybeans derived from low-fat soymilk. It is possible to obtain a more delicious low-fat soymilk lactic acid fermented product by further removing the flavor from the lactic acid fermentation while imparting a favorable flavor such as the taste and richness derived from sake lees.
本発明の製造方法における乳酸発酵で用いる菌は、好ましくは、ラクトバチルス・ブレビス(Lb.brevis)、ラクトバチルス・アシドフィルス(Lb.acidophilus)、ラクトバチルス・カゼイ(Lb.casei)、ラクトバチルス・ロイテリ(Lb.reuteri)、ラクトバチルス・デルブルッキー・サブスピーシーズ・ブルガリカス(Lb.delbruekii ssp.bulgaricus)、ラクトバチルス・プランタラム(Lb.plantarum)、ラクトバチルス・ブフネリ(Lb.buchneri)、ペディオコッカス・アシドラクティシ(Ped.acidilactici)、ラクトバチルス・ヘルベティカス(Lb.helveticus)等のラクトバチルス属細菌を用いる。 The bacteria used in the lactic acid fermentation in the production method of the present invention are preferably Lactobacillus brevis (Lb.brevis), Lactobacillus acidophilus (Lb.acidophilus), Lactobacillus casei (Lb.casei), Lactobacillus reuteri. (Lb.reuteri), Lactobacillus delbruekii ssp.bulgaricus, Lb.plantarum, Lb.buchneri, Pedio Coccus -Use Lactobacillus bacteria such as Ped.acidilactici and Lb.helveticus.
本発明の製造方法における乳酸発酵で用いる菌は、好ましくは、ストレプトコッカス・サーモフィルス(Streptococcus thermophilus)等のストレプトコッカス属細菌を用いる。 As the bacterium used in the lactic acid fermentation in the production method of the present invention, a bacterium belonging to the genus Streptococcus such as Streptococcus thermophilus is preferably used.
本発明の製造方法における乳酸発酵で用いる菌は、好ましくは、ラクトコッカス・ラクチス(Lactococcus lactis)、ラクトコッカス・ラクチス・サブスピーシーズ.ラクチス(Lactococcus lactis ssp.lactis)、ラクトコッカス・ラクチス・サブスピーシーズ.クレモリス(Lactococcus lactis ssp.cremoris)等のラクトコッカス属細菌を用いる。 The bacteria used in the lactic acid fermentation in the production method of the present invention are preferably Lactococcus lactis, Lactococcus lactis, and Lactococcus subspecies. Lactococcus lactis ssp.lactis, Lactococcus lactis subspecies. Bacteria of the genus Lactococcus such as Cremoris (Lactococcus lactis ssp.cremoris) are used.
本発明の製造方法における乳酸発酵で用いる菌は、好ましくは、ロイコノストク属細菌、エンテロコッカス・フェカリス(Enterococcus faecalis)等のエンテロコッカス属細菌を用いる。
本発明の製造方法における乳酸発酵では、上記選ばれる一種又は二種以上の菌を使用することができる。
As the bacterium used in the lactic acid fermentation in the production method of the present invention, a bacterium of the genus Enterococcus such as a bacterium of the genus Leukonostoc and Enterococcus faecalis is preferably used.
In the lactic acid fermentation in the production method of the present invention, one or more of the above-selected bacteria can be used.
乳酸発酵は、例えば、低脂肪豆乳又は酒粕を添加した低脂肪豆乳を含む原料へ、乳酸菌を添加して行う。
乳酸菌の割合は、発酵原料として、低脂肪豆乳又は酒粕を添加した低脂肪豆乳を含む原料100重量部に対して、好ましくは、0.01重量部〜20重量部程度、より好ましくは、0.1重量部〜15重量部程度の範囲である。
上記範囲において、乳酸発酵性が良く、貯蔵安定性も良い。
乳酸発酵の温度は、菌種によって適宜設定する。乳酸発酵の温度は、乳酸を効率よく生産させる(乳酸発酵性が良い)という観点から、好ましくは、5℃〜50℃程度の範囲であり、より好ましくは、10℃〜50℃程度、更に好ましくは、20℃〜48℃程度、特に好ましくは、30℃〜45℃程度である。例えば、30℃付近で培養する。
乳酸発酵の時間は、菌種によって適宜設定する。
乳酸発酵の時間は、乳酸を効率よく生産させる(乳酸発酵性が良い)という観点から、好ましくは、1時間〜30時間程度範囲であり、より好ましくは2時間〜20時間程度、更に好ましくは、3時間〜15時間程度、特に好ましくは、4時間〜8時間程度である。
乳酸発酵は、通常、静置発酵で行われるが、発酵液の温度分布を均一化する目的や菌体の沈殿を制御する目的で緩慢な攪拌を行っても良い。
Lactic acid fermentation is carried out, for example, by adding lactic acid bacteria to a raw material containing low-fat soymilk or low-fat soymilk to which sake lees are added.
The ratio of lactic acid bacteria is preferably about 0.01 parts by weight to 20 parts by weight, more preferably 0. The range is about 1 part by weight to 15 parts by weight.
In the above range, lactic acid fermentability is good and storage stability is also good.
The temperature of lactic acid fermentation is appropriately set depending on the bacterial species. The temperature of lactic acid fermentation is preferably in the range of about 5 ° C. to 50 ° C., more preferably about 10 ° C. to 50 ° C., and further preferably about 10 ° C. to 50 ° C. from the viewpoint of efficiently producing lactic acid (good lactic acid fermentability). Is about 20 ° C. to 48 ° C., particularly preferably about 30 ° C. to 45 ° C. For example, incubate at around 30 ° C.
The lactic acid fermentation time is appropriately set depending on the bacterial species.
The lactic acid fermentation time is preferably in the range of about 1 hour to 30 hours, more preferably about 2 hours to 20 hours, and further preferably about 2 hours to 20 hours, from the viewpoint of efficiently producing lactic acid (good lactic acid fermentability). It is about 3 hours to 15 hours, particularly preferably about 4 hours to 8 hours.
Lactic acid fermentation is usually carried out by static fermentation, but slow stirring may be carried out for the purpose of making the temperature distribution of the fermentation broth uniform or controlling the precipitation of bacterial cells.
本発明の低脂肪豆乳乳酸発酵物の製造方法は、低脂肪豆乳に酒粕を添加後乳酸発酵の前、及び/又は低脂肪豆乳に酒粕を添加し乳酸発酵の後、又は乳酸発酵した低脂肪豆乳に、酒粕を添加後、加圧均質化処理する。
均質化とは、低脂肪豆乳、および酒粕を十分混合することにより均質にすることであって、低脂肪豆乳や酒粕に含まれるたんぱく質や食物繊維などの粒子を機械的に微細化し、均一な分散状態にすることをいう。
加圧均質化処理は、特に限定されないが、低脂肪豆乳や酒粕に含まれるたんぱく質や食物繊維などの粒子を良好に均質化する観点から、ホモゲナイザーを用いることが好ましい。
加圧均質化処理は、たとえば、3MPa以上30MPa以下の圧力を加えながら、均質化することが好ましく、より好ましくは、5MPa以上25MPa以下である。
The method for producing a low-fat soymilk lactic acid fermented product of the present invention is low-fat soymilk after adding sake lees to low-fat soymilk and before lactic acid fermentation, and / or after adding sake lees to low-fat soymilk and lactic acid fermentation, or lactic acid-fermented low-fat soymilk. After adding soymilk, it is subjected to pressure homogenization treatment.
Homogenization is to homogenize by sufficiently mixing low-fat soymilk and sake lees, and mechanically finely disperse particles such as protein and dietary fiber contained in low-fat soymilk and sake lees. It means to put it in a state.
The pressure homogenization treatment is not particularly limited, but it is preferable to use a homogenizer from the viewpoint of satisfactorily homogenizing particles such as proteins and dietary fibers contained in low-fat soymilk and sake lees.
In the pressure homogenization treatment, for example, homogenization is preferably performed while applying a pressure of 3 MPa or more and 30 MPa or less, and more preferably 5 MPa or more and 25 MPa or less.
本発明の低脂肪豆乳乳酸発酵物の製造方法は、必要により殺菌を行う。
殺菌方法は、特に制限されないが、例えば、原料液に高温高圧水もしくは高圧水蒸気を注入するか、または原料液に対し通電によるジュール加熱、高周波(マイクロ波)による加熱などの直接加熱法や、電磁誘導加熱、プレート加熱、掻き取り式熱交換器、湯煎などの間接加熱法を単独でまたは組み合わせる方法が挙げられる。
The method for producing a low-fat soymilk lactic acid fermented product of the present invention sterilizes if necessary.
The sterilization method is not particularly limited, but for example, a direct heating method such as injecting high-temperature high-pressure water or high-pressure steam into the raw material liquid, Joule heating by energizing the raw material liquid, heating by high frequency (microwave), or electromagnetic waves. Indirect heating methods such as induction heating, plate heating, scraping heat exchangers, and sterilization can be used alone or in combination.
本発明の製造方法で得られた低脂肪豆乳乳酸発酵物は、大豆の風味が改善され、動物性の食の素材には無い、コク・うまみを有し、かつ、ざらつきや、口残りが無い、なめらかで口溶けの良い低脂肪豆乳乳酸発酵物であり、豆乳発酵飲料、豆乳ヨーグルト、豆乳フィリング等の幅広い飲料および食品用途に用いることができる。 The low-fat soymilk lactic acid fermented product obtained by the production method of the present invention has improved soybean flavor, has richness and taste that are not found in animal food materials, and has no roughness or residue. It is a low-fat soymilk lactic acid fermented product that is smooth and melts in the mouth, and can be used in a wide range of beverages and food applications such as fermented soymilk beverages, soymilk yogurt, and soymilk filling.
以下、本発明について実施例を示し、より詳細に説明する。なお、例中の数字は特に断りのない限り、重量基準を意味する。 Hereinafter, the present invention will be described in more detail with reference to examples. Unless otherwise specified, the numbers in the examples mean weight standards.
(実施例1)
低脂肪豆乳(「美味投入」不二製油(株)製、固形分:90.2%、蛋白質:5.1%、脂質:0.5%)を80部、市販の酒粕(固形分含量50%)(大関(株)製)を10部、水を10部を混合し、60℃で5分間加熱撹拌した後、70〜80℃で殺菌後24℃まで冷却した。
該調合液に対してラクトバチルス・ブルガリカスおよびストレプトコッカス・サーモフィラスを混合した乳酸菌スターターを0.01%添加して37〜40℃で約5時間発酵させた。
発酵後、ホモゲナイザーで5MPaで均質化処理し、70〜80℃で殺菌後24℃まで冷却し、低脂肪豆乳乳酸発酵物を得た。
(Example 1)
80 parts of low-fat soymilk (manufactured by "Delicious" Fuji Oil Co., Ltd., solid content: 90.2%, protein: 5.1%, fat: 0.5%), commercially available sake lees (solid content content 50) %) (10 parts manufactured by Ozeki Co., Ltd.) and 10 parts of water were mixed, heated and stirred at 60 ° C. for 5 minutes, sterilized at 70-80 ° C., and then cooled to 24 ° C.
0.01% of a lactic acid bacterium starter containing Lactobacillus bulgaricus and Streptococcus thermophilus was added to the preparation, and the mixture was fermented at 37-40 ° C. for about 5 hours.
After fermentation, it was homogenized at 5 MPa with a homogenizer, sterilized at 70 to 80 ° C., and then cooled to 24 ° C. to obtain a low-fat soymilk lactic acid fermented product.
(実施例2)
低脂肪豆乳(「美味投入」不二製油(株)製、固形分:90.2%、蛋白質:5.1%、脂質:0.5%)を80部、市販の酒粕(固形分含量50%)(大関(株)製)を10部、水を10部を混合し、60℃で5分間加熱撹拌した後、ホモゲナイザーで5MPaで均質化処理し、70〜80℃で殺菌後24℃まで冷却した。
該調合液に対してラクトバチルス・ブルガリカスおよびストレプトコッカス・サーモフィラスを混合した乳酸菌スターターを0.01%添加して37〜40℃で約5時間発酵させた。
得られた低脂肪豆乳乳酸発酵物のpHは4.8であった。
(Example 2)
80 parts of low-fat soymilk (manufactured by "Delicious" Fuji Oil Co., Ltd., solid content: 90.2%, protein: 5.1%, fat: 0.5%), commercially available sake lees (solid content content 50) %) (Made by Ozeki Co., Ltd.), 10 parts of water, 10 parts of water are mixed, heated and stirred at 60 ° C. for 5 minutes, homogenized at 5 MPa with a homogenizer, sterilized at 70-80 ° C., and then up to 24 ° C. Cooled.
0.01% of a lactic acid bacterium starter containing Lactobacillus bulgaricus and Streptococcus thermophilus was added to the preparation, and the mixture was fermented at 37-40 ° C. for about 5 hours.
The pH of the obtained low-fat soymilk lactic acid fermented product was 4.8.
(実施例3)
低脂肪豆乳(「美味投入」不二製油(株)製、固形分:90.2%、蛋白質:5.1%、脂質:0.5%)に対してラクトバチルス・ブルガリカスおよびストレプトコッカス・サーモフィラスを混合した乳酸菌スターターを0.01%添加して37〜40℃で約5時間発酵させた。
得られたpHは4.8の乳酸発酵低脂肪豆乳を80部、市販の酒粕(固形分含量50%)(大関(株)製)を10部、水を10部を混合し、60℃で5分間加熱撹拌した後、ホモゲナイザーで5MPaで均質化処理し、70〜80℃で殺菌後24℃まで冷却し、低脂肪豆乳乳酸発酵物を得た。
(Example 3)
Lactobacillus bulgaricus and Streptococcus thermophilus against low-fat soymilk (manufactured by Fuji Oil Co., Ltd., solid content: 90.2%, protein: 5.1%, fat: 0.5%) 0.01% of the lactic acid bacterium starter mixed with the above was added and fermented at 37-40 ° C. for about 5 hours.
The obtained pH is a mixture of 80 parts of 4.8 lactic acid fermented low-fat soymilk, 10 parts of commercially available sake lees (solid content 50%) (manufactured by Ozeki Co., Ltd.), and 10 parts of water at 60 ° C. After heating and stirring for 5 minutes, homogenization treatment was performed at 5 MPa with a homogenizer, sterilization at 70 to 80 ° C., and then cooling to 24 ° C. to obtain a low-fat soymilk lactic acid fermentation product.
(実施例4)
低脂肪豆乳(「美味投入」不二製油(株)製、固形分:90.2%、蛋白質:5.1%、脂質:0.5%)を65部、市販の酒粕(固形分含量50%)(大関(株)製)を30部、水を5部を混合し、60℃で5分間加熱撹拌した後、70〜80℃で殺菌後24℃まで冷却した。
該調合液に対してラクトバチルス・ブルガリカスおよびストレプトコッカス・サーモフィラスを混合した乳酸菌スターターを0.01%添加して37〜40℃で約5時間発酵させた。
発酵後、ホモゲナイザーで5MPaで均質化処理し、70〜80℃で殺菌後24℃まで冷却し、低脂肪豆乳乳酸発酵物を得た。
(Example 4)
65 parts of low-fat soymilk (manufactured by "Delicious" Fuji Oil Co., Ltd., solid content: 90.2%, protein: 5.1%, fat: 0.5%), commercially available sake lees (solid content content 50) %) (Made by Ozeki Co., Ltd.) was mixed with 30 parts and 5 parts with water, and the mixture was heated and stirred at 60 ° C. for 5 minutes, sterilized at 70 to 80 ° C., and then cooled to 24 ° C.
0.01% of a lactic acid bacterium starter containing Lactobacillus bulgaricus and Streptococcus thermophilus was added to the preparation, and the mixture was fermented at 37-40 ° C. for about 5 hours.
After fermentation, it was homogenized at 5 MPa with a homogenizer, sterilized at 70 to 80 ° C., and then cooled to 24 ° C. to obtain a low-fat soymilk lactic acid fermented product.
(実施例5)
低脂肪豆乳(「美味投入」不二製油(株)製、固形分:90.2%、蛋白質:5.1%、脂質:0.5%)を80部、市販の酒粕(固形分含量50%)(大関(株)製)を10部、水を10部を混合し、60℃で5分間加熱撹拌した後、70〜80℃で殺菌後24℃まで冷却した。
該調合液に対して乳酸菌スターターとしてストレプトコッカス・サーモフィラスを0.01%添加して37〜40℃で約5時間発酵させた。
発酵後、ホモゲナイザーで5MPaで均質化処理し、70〜80℃で殺菌後24℃まで冷却し、低脂肪豆乳乳酸発酵物を得た。
(Example 5)
80 parts of low-fat soymilk (manufactured by "Delicious" Fuji Oil Co., Ltd., solid content: 90.2%, protein: 5.1%, fat: 0.5%), commercially available sake lees (solid content content 50) %) (10 parts manufactured by Ozeki Co., Ltd.) and 10 parts of water were mixed, heated and stirred at 60 ° C. for 5 minutes, sterilized at 70-80 ° C., and then cooled to 24 ° C.
0.01% of Streptococcus thermophilus was added to the preparation as a lactic acid bacterium starter, and the mixture was fermented at 37 to 40 ° C. for about 5 hours.
After fermentation, it was homogenized at 5 MPa with a homogenizer, sterilized at 70 to 80 ° C., and then cooled to 24 ° C. to obtain a low-fat soymilk lactic acid fermented product.
(実施例6)
低脂肪豆乳(「美味投入」不二製油(株)製、固形分:90.2%、蛋白質:5.1%、脂質:0.5%)を80部、市販の酒粕(固形分含量50%)(大関(株)製)を10部、水を10部を混合し、60℃で5分間加熱撹拌した後、70〜80℃で殺菌後24℃まで冷却した。
該調合液に対してラクトバチルス・ブルガリカスおよびストレプトコッカス・サーモフィラスを混合した乳酸菌スターターを0.01%添加して37〜40℃で約5時間発酵させた。
発酵後、ホモゲナイザーで15MPaで均質化処理し、70〜80℃で殺菌後24℃まで冷却し、低脂肪豆乳乳酸発酵物を得た。
(Example 6)
80 parts of low-fat soymilk (manufactured by "Delicious" Fuji Oil Co., Ltd., solid content: 90.2%, protein: 5.1%, fat: 0.5%), commercially available sake lees (solid content content 50) %) (10 parts manufactured by Ozeki Co., Ltd.) and 10 parts of water were mixed, heated and stirred at 60 ° C. for 5 minutes, sterilized at 70-80 ° C., and then cooled to 24 ° C.
0.01% of a lactic acid bacterium starter containing Lactobacillus bulgaricus and Streptococcus thermophilus was added to the preparation, and the mixture was fermented at 37-40 ° C. for about 5 hours.
After fermentation, it was homogenized at 15 MPa with a homogenizer, sterilized at 70 to 80 ° C., and then cooled to 24 ° C. to obtain a low-fat soymilk lactic acid fermented product.
(実施例7)
低脂肪豆乳(「美味投入」不二製油(株)製、固形分:90.2%、蛋白質:5.1%、脂質:0.5%)に、豆乳クリーム(不二製油(株)製、固形分:81.0%、蛋白質:5.6%、脂質:12.3%)を加え、脂質2.3%に調整した低脂肪豆乳+豆乳クリームの混合液を80部、市販の酒粕(固形分含量50%)(大関(株)製)を10部、水を10部を混合し、60℃で5分間加熱撹拌した後、70〜80℃で殺菌後24℃まで冷却した。
該調合液に対してラクトバチルス・ブルガリカスおよびストレプトコッカス・サーモフィラスを混合した乳酸菌スターターを0.01%添加して37〜40℃で約5時間発酵させた。
発酵後、ホモゲナイザーで5MPaで均質化処理し、70〜80℃で殺菌後24℃まで冷却し、低脂肪豆乳乳酸発酵物を得た。
(Example 7)
Low-fat soymilk ("delicious" made by Fuji Oil Co., Ltd., solid content: 90.2%, protein: 5.1%, fat: 0.5%) and soymilk cream (manufactured by Fuji Oil Co., Ltd.) , Solid content: 81.0%, Protein: 5.6%, Lipid: 12.3%), 80 parts of a mixture of low-fat soymilk + soymilk cream adjusted to 2.3% fat, commercially available sake lees 10 parts of (solid content 50%) (manufactured by Ozeki Co., Ltd.) and 10 parts of water were mixed, heated and stirred at 60 ° C. for 5 minutes, sterilized at 70 to 80 ° C., and cooled to 24 ° C.
0.01% of a lactic acid bacterium starter containing Lactobacillus bulgaricus and Streptococcus thermophilus was added to the preparation, and the mixture was fermented at 37-40 ° C. for about 5 hours.
After fermentation, it was homogenized at 5 MPa with a homogenizer, sterilized at 70 to 80 ° C., and then cooled to 24 ° C. to obtain a low-fat soymilk lactic acid fermented product.
(比較例1)
実施例1で使用した酒粕に代えて、デキストリン5部、水を5部を使用した以外は、実施例1の配合および製法と同様にして、低脂肪豆乳乳酸発酵物を得た。
(Comparative Example 1)
A low-fat soymilk lactic acid fermented product was obtained in the same manner as in the formulation and production method of Example 1 except that 5 parts of dextrin and 5 parts of water were used instead of the sake lees used in Example 1.
(比較例2)
低脂肪豆乳(「美味投入」不二製油(株)製、固形分:90.2%、蛋白質:5.1%、脂質:0.5%)を80部、市販の酒粕(固形分含量50%)(大関(株)製)を10部、水を10部を混合し、60℃で5分間加熱撹拌した後、ホモゲナイザーで5MPaで均質化処理し、70〜80℃で殺菌後24℃まで冷却し、低脂肪豆乳組成物を得た。
(Comparative Example 2)
80 parts of low-fat soymilk (manufactured by "Delicious" Fuji Oil Co., Ltd., solid content: 90.2%, protein: 5.1%, fat: 0.5%), commercially available sake lees (solid content content 50) %) (Made by Ozeki Co., Ltd.), 10 parts of water, 10 parts of water are mixed, heated and stirred at 60 ° C. for 5 minutes, homogenized at 5 MPa with a homogenizer, sterilized at 70-80 ° C., and then up to 24 ° C. The mixture was cooled to obtain a low-fat soymilk composition.
(比較例3)
低脂肪豆乳(「美味投入」不二製油(株)製、固形分:90.2%、蛋白質:5.1%、脂質:0.5%)を80部、市販の酒粕(固形分含量50%)(大関(株)製)を10部、水を10部を混合し、60℃で5分間加熱撹拌した後、70〜80℃で殺菌後24℃まで冷却した。
該調合液に対してラクトバチルス・ブルガリカスおよびストレプトコッカス・サーモフィラスを混合した乳酸菌スターターを0.01%添加して37〜40℃で約5時間発酵し、低脂肪豆乳組成物を得た。
(Comparative Example 3)
80 parts of low-fat soymilk (manufactured by "Delicious" Fuji Oil Co., Ltd., solid content: 90.2%, protein: 5.1%, fat: 0.5%), commercially available sake lees (solid content content 50) %) (10 parts manufactured by Ozeki Co., Ltd.) and 10 parts of water were mixed, heated and stirred at 60 ° C. for 5 minutes, sterilized at 70-80 ° C., and then cooled to 24 ° C.
A low-fat soymilk composition was obtained by adding 0.01% of a lactic acid bacterium starter obtained by mixing Lactobacillus bulgaricus and Streptococcus thermophilus to the preparation and fermenting at 37-40 ° C. for about 5 hours.
(比較例4)
無調整豆乳(「無調整豆乳」不二製油(株)製、固形分:90.3%、蛋白質:4.4%、脂質:3.0%)を80部、市販の酒粕(固形分含量50%)(大関(株)製)を10部、水を10部を混合し、60℃で5分間加熱撹拌した後、70〜80℃で殺菌後24℃まで冷却した。
該調合液に対してラクトバチルス・ブルガリカスおよびストレプトコッカス・サーモフィラスを混合した乳酸菌スターターを0.01%添加して37〜40℃で約5時間発酵させた。
発酵後、ホモゲナイザーで5MPaで均質化処理し、70〜80℃で殺菌後24℃まで冷却し、豆乳乳酸発酵物を得た。
(Comparative Example 4)
80 parts of unadjusted soymilk ("Unadjusted soymilk" manufactured by Fuji Oil Co., Ltd., solid content: 90.3%, protein: 4.4%, fat: 3.0%), commercially available sake lees (solid content content) 50%) (manufactured by Ozeki Co., Ltd.) was mixed with 10 parts and 10 parts with water, and the mixture was heated and stirred at 60 ° C. for 5 minutes, sterilized at 70 to 80 ° C., and then cooled to 24 ° C.
0.01% of a lactic acid bacterium starter containing Lactobacillus bulgaricus and Streptococcus thermophilus was added to the preparation, and the mixture was fermented at 37-40 ° C. for about 5 hours.
After fermentation, it was homogenized at 5 MPa with a homogenizer, sterilized at 70 to 80 ° C., and then cooled to 24 ° C. to obtain a soymilk lactic acid fermented product.
官能試験では、下記に示す5段階で評価して評点を付け、15名の訓練された専門パネラーによる平均点を評価点とした。
大 豆 臭:弱 い 1〜5 強 い 合格基準(2以下が良好)
発 酵 臭:弱 い 1〜5 強 い 合格基準(4以上が良好)
旨 味 :弱 い 1〜5 強 い 合格基準(4以上が良好)
なめらかさ:ざらつく 1〜5 なめらか 合格基準(3以上が良好)
雑 味 :弱 い 1〜5 強 い 合格基準(2以下が良好)
実施例1〜7および比較例1〜4の結果を<表>に示す。
In the sensory test, evaluation points were given on a scale of 5 as shown below, and the average score of 15 trained professional panelists was used as the evaluation point.
Large bean odor: Weak 1-5 Strong Passing criteria (2 or less is good)
Yeast odor: weak 1-5 strong acceptance criteria (4 or more is good)
Taste: Weak 1-5 Strong Passing criteria (4 or above is good)
Smoothness: Roughness 1-5 Smoothness Pass criteria (3 or more is good)
Miscellaneous: Weak 1-5 Strong Passing criteria (2 or less is good)
The results of Examples 1 to 7 and Comparative Examples 1 to 4 are shown in <Table>.
(実施例8)
実施例1で得られた低脂肪豆乳乳酸発酵物70部に対して、グラニュー糖10部、ペクチン1部、水19部を加えて100部とし、80〜90℃で加熱撹拌して殺菌し、次いで2MPaの圧力にてホモゲナイザーで均質化し、冷却して低脂肪豆乳ヨーグルトを得た。
得られた豆乳ヨーグルトは、なめらかなペースト状の物性を示し、かつ乳原料から得られるヨーグルトには無い、独特のコク・うまみを有し、かつ、ざらつきや、口残りが無い、なめらかで口溶けの良い風味であった。
また、水の替わりに「ブルーベリー果汁」などの各種果汁を用いることで、バラエティ豊富な低脂肪豆乳ヨーグルトが提供できる。
(Example 8)
To 70 parts of the low-fat soymilk lactic acid fermented product obtained in Example 1, 10 parts of granulated sugar, 1 part of pectin, and 19 parts of water were added to make 100 parts, and the mixture was sterilized by heating and stirring at 80 to 90 ° C. Then, it was homogenized with a homogenizer at a pressure of 2 MPa and cooled to obtain low-fat soymilk yogurt.
The obtained soymilk yogurt shows smooth paste-like physical properties, has a unique richness and umami that yogurt obtained from dairy ingredients does not have, and has no roughness or residue, and is smooth and melts in the mouth. It had a good flavor.
In addition, by using various fruit juices such as "blueberry juice" instead of water, a wide variety of low-fat soymilk yogurt can be provided.
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