JP2021108772A - Vinyl isolator for animal operation - Google Patents

Abstract

To provide a simple animal operation method to give birth aseptically without euthanizing an experimental animal or a domestic animal; and to provide a device therefor.SOLUTION: Aseptic childbirth is performed by performing caesarean section of an animal 320 by using gloves 150, via a surgical window, in a vinyl isolator 100 for animal operation, with a window for a surgical field in a window frame 120 for the surgical field.SELECTED DRAWING: Figure 10-2

Description

The present invention relates to vinyl isolators for animal surgery.

When experimental animals are contaminated with specific microorganisms such as pathogenic microorganisms, the reproductive performance and experimental results are greatly affected. Conventionally, a caesarean section and a uterine cutting method are known as methods for removing pathogenic microorganisms from contaminated animals.

Artificial delivery of small animals has generally been performed by hysterectomy. To name a common hysterectomy
(i) The process of euthanizing the mother due to dislocation of the cervical spine, etc.
(ii) The process of stopping the cervical spine of an animal with forceps and immersing it in a tank of disinfectant such as iodine tincture.
(iii) After disinfection, the process of pulling up the animal from the iodine tincture tank and wiping off the disinfectant solution on the surface of the animal,
(iv) The process of placing the animal on the dissection table, lying on its back, and fixing the limbs,
(v) Pinch the abdominal muscles in the center of the abdomen with tweezers, and cut the epidermis and dermis at the same time along the midline with scissors to make an incision to the xiphoid process.
(vi) The process of removing the uterus,
(vii) The process of stopping the left and right fallopian tubes and the vaginal side with forceps,
(viii) The process of cutting the outside of the forceps part that stopped the fallopian tube and the vaginal side with scissors,
(ix) The process of removing the uterus with the uterus stopped with forceps,
(x) A step of immersing the removed uterus in a disinfectant solution to disinfect it is involved. Then, in general, the removed uterus is copied into a clean bench or a vinyl isolator.
(xi) The process of removing the fallopian tubes and forceps on the vaginal side of the uterus and simultaneously incising the uterus and amniotic membrane.
(xii) A process in which the uterus is washed with peracetic acid, etc., passed through a chemical bath, and then the fetus is removed.
(xiii) The process of wiping and resuscitating the amniotic fluid in the body, oral cavity, and nostrils of the fetus (newborn) is performed.

As described above, in hysterectomy, the number of steps up to hysterectomy is large, and it is difficult to arrange all the instruments in the vinyl isolator and perform fine work. It is also known that the inside of the uterus is basically a sterile environment. Therefore, a method has been adopted in which the work up to the hysterectomy is performed in a clean bench, the removed uterus is moved into a vinyl isolator or a clean bench, and resuscitation is performed.

However, in hysterectomy, even if the mother's womb is euthanized or anesthetized, it cannot give birth again, which is not always desirable in terms of laboratory animal ethics. For example, in animal experiments, the 3Rs of "Replacement," "Reduction," and "Refinement" have been proposed. Reduction is the idea of reducing the number of animals used and using the minimum number of animals scientifically required. Refinement is a philosophy that includes reducing pain, taking measures such as euthanasia, and improving the breeding environment.

On the other hand, with Caesarean section, it is possible to give birth again without having to euthanize the mother's womb. This is in line with the above 3R philosophy. However, in cesarean section, if the mother's womb is not a germ-free animal, there is a high possibility that contamination will occur even if the non-sterile mother's womb is brought into a vinyl isolator as it is and the work is performed. Also, it is not easy to completely sterilize a non-sterile mother's womb and place it in a vinyl isolator. Further, even if the sterilization is successful, it is not desirable in terms of cost because the equipment becomes large and the work load is large in order to perform the entire process of cesarean section in the vinyl isolator.

There has been a need for simple methods and devices for aseptically giving birth to animals, such as laboratory animals or livestock animals.

An object of the present invention is to solve the above problems at least partially. That is, it is an object of the present invention to provide a simple animal surgery method and an apparatus therefor.

The present inventors have diligently studied the above problems. In adopting the Caesarean section instead of hysterectomy, one idea is to cut the experimental animal together with the vinyl isolator. However, with this method, the vinyl isolator becomes disposable after each birth, which can be costly and waste resources. Therefore, as a result of further studies, the present inventors have found that, as an example, a caesarean section can be easily performed on an animal by using a vinyl isolator having a window for a surgical field, and this is included as an embodiment. The invention was completed.

Furthermore, the present inventors have developed a novel method that when a caesarean section is performed on an animal using a vinyl isolator having a window for surgical field, the vinyl isolator undulates and the light is diffusely reflected, making it difficult to perform detailed work. I ran into a challenge. Therefore, as a result of further studies, the present inventors have found that, as an example, by providing an observation window separate from the surgical field window in the vinyl isolator, animals can be observed without any trouble such as diffused reflection of light, and the operation can be performed quickly. The present invention including this as an embodiment has been completed.

The present disclosure includes the following embodiments.
[1] A vinyl isolator having a surgical field window 110 for performing a caesarean section on an animal.
[2] The vinyl isolator according to the first embodiment, wherein the surgical field window 110 has a surgical field window frame 120 and a double-sided adhesive film 115.
[3] The vinyl isolator according to the second embodiment, wherein the double-sided adhesive film 115 is transparent soft vinyl chloride.
[4] The vinyl isolator according to any one of embodiments 1 to 3, further comprising an observation window 130.
[5] The vinyl isolator according to any one of embodiments 1 to 4, which is further connected to the resuscitation vinyl isolator 200 via a connecting portion 210.
[6] The vinyl isolator according to the fifth embodiment, wherein the resuscitation vinyl isolator 200 has an observation window 130.
[7] The vinyl isolator according to any one of embodiments 4 to 6, wherein the observation window 130 has an observation window frame 135 and a hard window material 140.
[8] The vinyl isolator according to the seventh embodiment, wherein the hard window material 140 of the observation window 130 is transparent hard vinyl chloride.
[9] A method for producing a germ-free animal, which comprises performing a caesarean section on a non-human animal 320 using the vinyl isolator according to any one of embodiments 1-8.
[10] Caesarean section
(i) A step of bringing the abdomen of the animal 320 into close contact with the surgical field window 110 of the vinyl isolator 100 for animal surgery.
(ii) A step of incising the abdomen of the animal 320 together with the double-sided adhesive film 115 stretched on the window 110 for the surgical field from the inside of the vinyl isolator 100 for animal surgery using a scalpel.
(iii) A step of sandwiching the incised double-sided adhesive film 115 and the animal 320 from the inside of the vinyl isolator 100 for animal surgery with tweezers to clip them so that the sterile condition in the vinyl isolator is maintained.
(iv) The step of removing the fetus from the incised abdomen into the vinyl isolator 100 for animal surgery,
The production method according to the ninth embodiment.
[11] Before the step (i),
(i-1) The process of pre-shaving the abdominal hair of animal 320,
(i-2) The process of disinfecting the abdomen 330 of a shaved animal,
The production method according to the tenth embodiment, further comprising.
[12] A vinyl isolator 100 for performing cesarean section surgery on the animal 320 is connected to the resuscitation vinyl isolator 200 via a connecting portion 210, and further.
(v) A step of transferring the removed fetus to a vinyl isolator 200 for resuscitation, washing the surface of the fetus in the vinyl isolator 200 for resuscitation, and performing resuscitation.
The production method according to the tenth or eleventh embodiment.

As an effect of the present invention, a caesarean section can be easily applied to an animal.

It is a top view of the vinyl isolator 100 for animal surgery. The dimensions are exemplary. It is a front view of the vinyl isolator 100 for animal surgery. The dimensions are exemplary. It is a right side view of vinyl isolator 100 for animal surgery. The dimensions are exemplary. It is a bottom view of the vinyl isolator 100 for animal surgery. The dimensions are exemplary. It is a top view of the operating table 300. The dimensions are exemplary. It is a front view of the operating table 300. The dimensions are exemplary. It is a right side view of the operating table 300. It is a photograph of the caesarean section method using a vinyl isolator 100 for animal surgery. It is a photograph of the caesarean section method using a vinyl isolator 100 for animal surgery. An animal 320 with shaved abdomen is brought into close contact with the double-sided adhesive film 115. It is a photograph which connected vinyl isolator 100 for animal surgery and vinyl isolator 200 for resuscitation. It is a schematic diagram of the vinyl isolator 100 for animal surgery and the vinyl isolator 200 for resuscitation that were connected. The shaved abdomen 330 of the animal 320 is in close contact with the underside of the double-sided adhesive film 115. It is a photograph of vinyl isolator 100 for animal surgery. The two observation windows are indicated by arrows. It is a photograph from the front of the vinyl isolator 100 for animal surgery. The two observation windows 130 are indicated by arrows. It is a photograph of a conventional sterile pig-producing isolator 500 and a 4-week-old rearing isolator 400 that are linked. It is a photograph of a conventional sterile pig isolator 500. It is a photograph of hysterectomy using a conventional sterile porcine isolator 500.

Vinyl Isolators of the Invention In certain embodiments, the present invention provides a vinyl isolator 100 for animal surgery. The animal surgical vinyl isolator 100 has a surgical field window 110. In the present specification, the surgical field window 110 may also be referred to as a surgical window, a surgical frame, a surgical window or simply a surgical window, but these terms are synonymous. The surgical field window 110 has a surgical field window frame 120, and a double-sided adhesive film 115 can be adhered (stretched) to the surgical field window frame 120. That is, in certain embodiments, the surgical field window 110 has a surgical field window frame 120 and a double-sided adhesive film 115. In certain embodiments, the surgical field window 110 can be provided on the bottom or side of the animal surgical vinyl isolator 100. As a result, the animal can be brought into close contact with, for example, the bottom surface (the surface on the outside world side) of the vinyl isolator 100 for animal surgery. Examples of the double-sided adhesive film 115 include, but are not limited to, transparent soft vinyl chloride having an adhesive material or an adhesive material on both sides (for example, 0.02 mm thick, manufactured by Nippon Wave Block Co., Ltd.). The double-sided pressure-sensitive adhesive film 115, for example, transparent soft vinyl chloride, can have a thickness of 0.01 mm to 1 mm, 0.02 mm to 0.5 mm, for example 0.03 mm, for example 0.02 mm, for example 0.01 mm.

The dimensions of the animal surgical vinyl isolator 100 can be appropriately designed according to the size of the target animal (laboratory animal, domestic animal, etc.). In certain embodiments, the animal surgical vinyl isolator 100 is, for example, 500-5000 cm, 500-4000 cm, 500-3000 cm, 600-2500 cm, 700-2000 cm, 800 if the experimental animal is a marmoset or similar animal. ~ 1500 cm, for example 900-1200 cm length, for example 100-3000 cm, 150-2500 cm, 200-2000 cm, 250-1500 cm, for example 300-1200 cm width, for example 200-1000 cm, 300-900 cm, 400-800 cm, 500- It can have a height of 700 cm, for example 550-600 cm, but the dimensions are not limited to this.

In another embodiment, the animal surgical vinyl isolator 100 of the present invention can have an observation window 130. In the present specification, unless otherwise specified, the observation window 130 is a window different from the surgical field window 110. The observation window 130 has an observation window frame 135 and a hard window material 140. In a certain embodiment, the observation window 130 is formed by adhering transparent hard vinyl chloride (a thin plate having a thickness of 0.1 mm) as a hard window material 140 to the observation window frame 135 with an adhesive. The hard window material 140 can be a material having less diffuse reflection (diffuse reflection) of light. In certain embodiments, the rigid window material 140 can be, but is not limited to, a transparent vinyl chloride plate. In the present specification, less diffused reflection means less diffused reflection as compared with a wavy soft material such as a vinyl isolator, for example, less diffused reflection as shown by transparent hard vinyl chloride. Further, in the present specification, it is preferable that the hard window material 140 has less diffuse reflection of light, but at this time, the hard window material 140 is allowed to be specularly reflected. The observation window 130 can be configured by attaching or stretching the hard window material 140 to the observation window frame 135. Unlike soft materials such as vinyl isolators, the hard window material 140 does not undulate, so there is little diffused reflection of light, and in detailed work such as surgery, animals can be observed without problems such as light reflection, and surgery can be performed in a short time. Can be carried out.

In certain embodiments, the observation window 130 and the observation window frame 135 can be circular, such as substantially circular or perfectly circular. The observation window 130 and the observation window frame 135 do not necessarily have to be a perfect circle, and may be oval, quadrangular, rectangular, square, or the like depending on the shape of the animal and the usage mode of the device. In certain embodiments, the rigid window material 140 can be a rigid film, such as a vinyl plate. The observation window 130 and the observation window frame 135 may have any size as long as the animal can be observed through the vinyl isolator and a caesarean section can be performed. In certain embodiments, the observation window frame 135 can be, but is not limited to, a circle having a diameter (inner diameter) of, for example, 5 to 50 cm, 10 to 40 cm, for example, 20 to 30 cm. Some embodiments observed in Embodiment window frame 135, for example, ^{50cm 2 ~8000cm 2, 300cm 2 ~5000cm} 2, for example 1200 cm ² ～3000Cm oval having ^two about the area (inner area of the frame), square, rectangular, or square It can be a frame of, but it is not limited to this. In the present specification, the circular observation window frame 135 may be referred to as an observation window ring. In certain embodiments, the hard window material 140, such as a vinyl plate, such as a transparent hard vinyl chloride plate, is, for example, 0.01 mm to 10 mm, 0.05 mm to 5 mm, 0.1 mm to 3 mm, for example about 0.3 mm, for example about 0.2 mm, for example about. It can have a thickness of 0.1 mm.

In certain embodiments, the animal surgical vinyl isolator 100 may have one or more observation windows 130, for example two, three, or four. In certain embodiments, the observation window 130 can be provided on the top (top) and / or side (side) of the animal surgery vinyl isolator 100. In another embodiment, a part of the upper portion and the side portion of the vinyl isolator 100 for animal surgery can be inclined, and the observation window 130 can be provided on the inclined surface (also referred to as the inclined portion). In another embodiment, the observation window 130 can be provided on the top, sides, and / or slopes of the animal surgery vinyl isolator 100. The dimensions, shape, and material of each observation window 130 may be the same or different.

In certain embodiments, the animal surgical vinyl isolator 100 of the present invention may further include a resuscitation vinyl isolator 200 as part of its configuration. In another embodiment, the animal surgical vinyl isolator 100 of the present invention may have a connecting portion 210 for connecting to the resuscitation vinyl isolator 200. The animal surgery vinyl isolator 100 may be connected to the resuscitation vinyl isolator 200 via the connecting portion 210. In certain embodiments, the resuscitation vinyl isolator 200 has an observation window 130. The observation window 130 has an observation window frame 135 and a hard window material 140. The observation window frame 135 and the hard window material 140 can be the above-mentioned ones.

In certain embodiments, a table on which the animal is placed can be used to bring the animal into close contact with the bottom surface (the surface on the outside world side) of the animal surgery vinyl isolator 100. In the present specification, the table on which such an animal is placed may be referred to as an operating table 300 or a caesarean section holding table. The operating table 300 may have a height-adjustable mechanism, such as a jack 310. The jack 310 can be screw or hydraulic depending on the size of the animal. Examples of the jack 310 include, but are not limited to, pantograph jacks, floor jacks, and foldable jacks.

In certain embodiments, the animal surgical vinyl isolator 100 of the present invention may be fitted with gloves 150 for performing a caesarean section within the isolator. Two or more gloves 150, for example, two, three, four, five, six, seven, for example eight, can be attached, but the gloves 150 are not limited to this. The vinyl isolator 100 for animal surgery of the present invention may have a normal configuration of a vinyl isolator, for example, an opening / closing part for carrying in and out, an intake part for sending sterile air, and an exhaust part for sending out air. The opening / closing part for carrying in and out can also serve as a connecting part with the vinyl isolator for resuscitation. There may be one or more opening / closing parts.

Methods for Creating Germ-Free Animals by Caesarean Section In one embodiment, a method for producing germ-free animals is provided, which comprises performing a caesarean section on a non-human animal 320 using the vinyl isolator 100 for animal surgery of the present invention. In this production method, a set of sterilized surgical instruments can be placed inside the vinyl isolator 100 for animal surgery. Further, for example, when the animal 320 has body hair, the abdomen (abdominal surface) of the animal can be shaved in advance (step (i-1)). In addition, the abdomen 330 of the shaved animal can be disinfected (step (i-2)). In addition, the body surface of the animal may be disinfected in advance. These steps may be omitted if the animal does not have hair. Next, the animal 320 can be placed on the operating table 300 and the height of the animal 320 can be adjusted so that the abdomen of the animal 320 is brought into close contact with the surgical field window 110 of the vinyl isolator 100 for animal surgery (step (i)). ). In addition, the animal 320 can be anesthetized in advance. Next, from the inside of the vinyl isolator 100 for animal surgery, an incision is made in the abdomen of the animal 320 together with the double-sided adhesive film 115 stretched on the window 110 for the surgical field using a scalpel (step (ii)). In the present specification, incising the abdomen of the animal 320 together with the double-sided adhesive film 115 means incising the double-sided adhesive film 115 and the animal abdomen together. When making the incision, the animal abdomen is kept in close contact with the double-sided adhesive film 115, and the operation is performed so that the air outside the animal surgery vinyl isolator 100 does not enter the animal surgery vinyl isolator 100. Next, from the inside of the vinyl isolator 100 for animal surgery, the cut double-sided adhesive film 115 and the animal 320 are clipped with tweezers so that the sterile condition in the vinyl isolator is maintained (step (iii). )). Next, the fetus is removed from the incised abdomen into the vinyl isolator 100 for animal surgery (step (iv)). In some cases, the excised fetus (newborn) can be transferred to the resuscitation vinyl isolator 200, and the surface of the fetus (newborn) can be washed and resuscitated in the resuscitation vinyl isolator 200 (step v). The resuscitated fetus (newborn) can be bred for an appropriate period, for example, in a sterile environment. For breeding, for example, artificial feeding may be used.

The uterus of an animal is basically sterile. However, microorganisms are usually present in the intestine. If the intestine is injured at the time of incision, contamination is likely to occur. Therefore, the incision operation is preferably performed so as not to injure the intestine. In addition, after the abdominal incision and fetal removal, the mother's womb can be recovered at an early stage by suturing the incision. Since the uterus of the recovered mother's womb has not been removed, it is possible to give birth again, and it is possible to give birth multiple times from the same mother's womb.

The method of caesarean section of the present invention and the method of producing germ-free animals of the present invention can be performed on various animals. The animal 320 for performing the royal incision may be, for example, an experimental animal, a domestic animal, a wild animal, a genetically engineered animal, a genetically modified animal, a cloned animal, a genome editing animal, or the like, but is not limited thereto. In addition, the genetically engineered animals, genetically modified animals, cloned animals and genome-editing animals referred to here may include not only the first generation but also their offspring and strains. In certain embodiments, the animal 320 performing the caesarean section can be a mammal. Mammals include marmosets, mice, nude mice, rats, hamsters, guinea pigs, guinea pigs, ferrets, rabbits, dogs, cats, pigs, mini pigs, micro pigs, red-tailed monkeys, cynomolgus monkeys, chimpanzees, gorillas, cows, sheep, goats, Horses, etc. can be mentioned, but not limited to this. By the term animal 320 herein, humans shall be excluded. That is, in the present specification, non-human animals are simply referred to as animals.

With the vinyl isolator for animal surgery of the present invention, a sterile animal can be produced by performing a caesarean section aseptically from an animal that is not necessarily sterile. Also, the animals produced do not necessarily have to be completely sterile. For example, pigs can be used as animals if desired, and SPF (specific pathogen free) pigs can be produced by the vinyl isolator for animal surgery of the present invention. With the vinyl isolator for animal surgery of the present invention, the fetus can be removed without removing the uterus of the animal. In addition, the vinyl isolator for animal surgery of the present invention can remove the fetus without euthanizing the mother's womb. This is a particularly advantageous effect for animals for which cesarean section was difficult with conventional methods. That is, according to the present invention, the pain of animals can be alleviated, and the number of experimental animals to be euthanized can be reduced. These have favorable effects that are in line with the 3R philosophy of animal experiments.

The present invention will be described in more detail with reference to the following examples. The examples below are for illustration purposes only and should not be construed as limiting the invention in any way.

[Example 1]
Creation of a sterile marmoset by cesarean section A cesarean section was performed on the marmoset using a vinyl isolator 100 for animal surgery having a window 110 for the surgical field. A set of sterilized surgical instruments was placed inside the vinyl isolator 100 for animal surgery. After anesthetizing the marmoset, shaving its abdomen (abdominal surface) and disinfecting the abdomen, place it on the operating table 300, adjust the height of the operating table 300, and use the abdomen for the surgical field of the vinyl isolator 100 for animal surgery. It was brought into close contact with the window 110 (FIG. 9). Next, from the inside of the vinyl isolator 100 for animal surgery, an incision was made in the abdomen of the marmoset together with the double-sided adhesive film 115 (0.02 mm thick, manufactured by Nippon Wave Block Co., Ltd.) stretched on the window 110 for the surgical field using a scalpel. .. When making the incision, the abdomen of the marmoset was kept in close contact with the double-sided adhesive film 115, and an operation was performed so that the air outside the vinyl isolator 100 for animal surgery did not enter the vinyl isolator 100 for animal surgery. Next, from the inside of the vinyl isolator 100 for animal surgery, the cut double-sided adhesive film 115 and the animal 320 were clipped by sandwiching them with tweezers so that the sterile state inside the vinyl isolator was maintained. Next, marmoset pups were removed from the incised abdomen into a vinyl isolator 100 for animal surgery. Then, the excised marmoset pups were transferred to the resuscitation vinyl isolator 200, and the surface of the marmoset pups was washed and resuscitated in the resuscitation vinyl isolator 200. After resuscitation, marmoset pups were bred in vinyl isolators and were sterile.

[Example 2]
A caesarean section was performed on the marmoset using a vinyl isolator 100 for animal surgery having a window 110 for the surgical field and an observation window 130. The observation window 130 was formed by adhering transparent hard vinyl chloride (a thin plate with a thickness of 0.1 mm) to the observation window frame 135 with an adhesive. Caesarean section was performed in the same procedure as in Example 1 while observing the inside of the vinyl isolator 100 for animal surgery through the observation window 130. The removed marmoset pups were revived and bred in a vinyl isolator and were sterile.

[Comparison example]
Photographs of conventional sterile pig-producing isolators and 4-week-old rearing isolators are shown in FIGS. 13 and 14. In addition, a photograph of hysterectomy using a conventional sterile porcine isolator is shown in FIG. First, 1. Sterilize the isolator, 2. Start surgery. Then 3. Remove the uterus, 4. This is washed with peracetic acid. After that, 5. Through the chemical tank, 6. Start resuscitation. 7. Resuscitate and 8. After resuscitation, newborn pigs 9. Breeding. In the case of sterile pigs, breeding is generally carried out by artificial feeding up to 4 weeks of age. With conventional hysterectomy, the mother's womb had to be euthanized. Alternatively, the mother's womb was unable to re-pregnant and give birth.

INDUSTRIAL APPLICABILITY According to the present invention, germ-free animals and animals having no specific pathogen (SPF animals) can be produced without excising the uterus of the animal and without euthanizing the animal.

In this specification, documents including patent applications, patent specifications, and manufacturer's manuals may be cited. The disclosure of these documents is not considered to be relevant to the patentability of the invention, but is incorporated herein by reference in its entirety. More specifically, all referenced documents are incorporated herein by reference, as if each individual document was specifically and individually indicated to be incorporated by reference.

100 Animal Surgical Vinyl Isolator 110 Surgical Field Window 115 Double-sided Adhesive Film 120 Surgical Field Window Frame 130 Observation Window 135 Observation Window Frame 140 Hard Window Material 150 Gloves 200 Resuscitation Vinyl Isolator 210 Connection 300 Operating Table 310 Operating Table Jack 320 Animals 330 Shaved abdomen of animals 400 4-week-old rearing isolator 500 Sterile pig production isolator

Claims (12)

A vinyl isolator for performing a caesarean section on an animal, having a surgical field window 110. The vinyl isolator according to claim 1, wherein the surgical field window 110 has a surgical field window frame 120 and a double-sided adhesive film 115. The vinyl isolator according to claim 2, wherein the double-sided adhesive film 115 is transparent soft vinyl chloride. The vinyl isolator according to any one of claims 1 to 3, further comprising an observation window 130. The vinyl isolator according to any one of claims 1 to 4, further connected to the resuscitation vinyl isolator 200 via a connecting portion 210. The vinyl isolator according to claim 5, wherein the resuscitation vinyl isolator 200 has an observation window 130. The vinyl isolator according to any one of claims 4 to 6, wherein the observation window 130 has an observation window frame 135 and a hard window material 140. The vinyl isolator according to claim 7, wherein the hard window material 140 of the observation window 130 is transparent hard vinyl chloride. A method for producing a germ-free animal, which comprises performing a caesarean section on a non-human animal 320 using the vinyl isolator according to any one of claims 1 to 8. Caesarean section
(i) A step of bringing the abdomen of the animal 320 into close contact with the surgical field window 110 of the vinyl isolator 100 for animal surgery.
(ii) A step of incising the abdomen of the animal 320 together with the double-sided adhesive film 115 stretched on the window 110 for the surgical field from the inside of the vinyl isolator 100 for animal surgery using a scalpel.
(iii) A step of sandwiching the incised double-sided adhesive film 115 and the animal 320 from the inside of the vinyl isolator 100 for animal surgery with tweezers to clip them so that the sterile condition in the vinyl isolator is maintained.
(iv) The step of removing the fetus from the incised abdomen into the vinyl isolator 100 for animal surgery,
9. The production method according to claim 9. Before step (i),
(i-1) The process of pre-shaving the abdominal hair of animal 320,
(i-2) The process of disinfecting the abdomen 330 of a shaved animal,
The production method according to claim 10, further comprising. A vinyl isolator 100 for performing cesarean section surgery on the animal 320 is connected to the resuscitation vinyl isolator 200 via a connecting portion 210, and further.
(v) A step of transferring the removed fetus to a vinyl isolator 200 for resuscitation, washing the surface of the fetus in the vinyl isolator 200 for resuscitation, and performing resuscitation.
The production method according to claim 10 or 11, which comprises.

Images