JP2021045054A - Antidepressant composition and happiness enhancing composition - Google Patents

Abstract

To provide an antidepressant composition or a happiness enhancing composition.SOLUTION: An antidepressant composition or a happiness enhancing composition includes a fermented substance of Lactobacillus helveticus as an active principle. The fermented substance may be fermented milk. The Lactobacillus helveticus may be Lactobacillus helveticus NITE BP-01671. The composition may be a beverage/food product composition.SELECTED DRAWING: None

Description

The present technology relates to a composition for antidepressant depression and a composition for improving happiness.

In recent years, the sophistication of technology, information, infrastructure, etc. has progressed rapidly. Along with these sophistication, people's living environment and working environment are also changing drastically. For this reason, people are required to adapt appropriately to such changes in the environment, but adapting to these changes tends to be a mental burden and tends to intensify emotional ups and downs. Others have a personality that makes it difficult for them to adapt to this change. For this reason, in modern society, people tend to feel depressed and less likely to feel satisfied.

For example, in Patent Document 1, a) improved vitality, b) specific cognitive function, in particular sustained attention and faster response time, and improved information processing; or c) improved sleep quality; and d) improved. A chicken egg white lysozyme hydrolyzate in which the composition has a tryptophan to large neutral amino acid ratio (“Trp / LNAA”) greater than 0.15, which provides a healthy adult consumer with a sensation of good health. Compositions have been proposed that include pharmaceuticals, foods or dietary supplements that provide a Trp hydrolyzate dose of 10 to 100 mg per day.

The specific content of anxiety varies, and the specific content of anxiety also differs for each subject. Therefore, as in Patent Document 1, the specific content of the subject can be met. Proposals for compositions that are more specific to the application are being made.

JP-A-2017-79744

Therefore, the main purpose of this technique is to provide a composition for antidepressant depression or for improving happiness.

As a result of diligent studies, the present inventor has found that the fermented product of Lactobacillus herbeticus has a particularly antidepressant effect and a happiness-enhancing effect based on a clinical trial of STAI (psychological test for measuring anxiety). Completed the invention. The present invention is as follows.

The present invention can provide an antidepressant composition or a happiness-enhancing composition containing a fermented product of Lactobacillus herbeticus as an active ingredient.
The fermented product may be fermented milk.
The Lactobacillus herbeticus may be Lactobacillus herbeticus NITE BP-01671.
The composition may be a food and drink composition.

The present technology can provide compositions for antidepressant or happiness enhancement. The effects described here are not necessarily limited, and may be any of the effects described in the present technology.

Hereinafter, suitable embodiments for carrying out the present technology will be described. It should be noted that the embodiments described below show an example of typical embodiments of the present technology, and the scope of the present technology is not narrowly interpreted by this. In this specification, the percentage is expressed by mass unless otherwise specified. Further, the upper limit value and the lower limit value can be arbitrarily combined as desired.

<1. Fermented product of Lactobacillus herbeticus in this technology>
The present technology can provide a composition for antidepressant and / or for improving happiness, which contains a fermented product of Lactobacillus helveticus as an active ingredient.
In the present specification, "depression" means "depressed feelings that do not swell", "feelings of sadness that are depressed", "full of sadness and disappointment", "pessimistic feelings of lack of competence", and inactivity. A mental state characterized by depression. "
The term "happiness" as used herein refers to "a feeling of fullness,""a state of being full of happiness and joy,""a feeling of fulfillment and satisfaction," and the like.

The fermented product of Lactobacillus herveticas in this technology is excellent in safety because its active ingredient is a fermented product using Lactobacillus herveticas, and there are concerns about side effects even if it is continuously administered for a long period of time. It is very useful because it is less necessary. Furthermore, it is highly safe when used in combination with other ingredients or other compositions.
The form of the fermented product of the present technology may be liquid, fluid, solid, powder or the like, but is not particularly limited.

The type of fermented product of the present technology is not particularly limited, but among them, fermented milk is suitable from the viewpoint of the effect of the present technology (particularly antidepressant action and happiness improving action), and the fermented milk is a food or drink. It can be used for a wide range of purposes such as pharmaceutical compositions as well as compositions. Further, it is more preferable to use fermented milk obtained by fermenting a milk component derived from milk from the viewpoint of the effect of the present technology.

The "fermented milk" of the fermented milk of Lactobacillus herveticas in the present technology is "a fermented product obtained by culturing a culture medium containing milk components using the fermented milk of Lactobacillus herveticas of the present technology", and is "milk". It is a broader concept than "fermented milk" in the narrow sense based on the "Ministry Ordinance on Ingredient Standards for Milk Products". Further, unless otherwise specified, the fermented milk of Lactobacillus herbeticus of the present technology may contain cells such as lactic acid bacteria and yeast, or may be any of the supernatants obtained by removing these cells.

<1-1. Method for producing fermented product of Lactobacillus herbeticus in this technology>
The fermented product of Lactobacillus herbeticus in the present technology can be produced by using a general fermentation method of lactic acid bacteria.
Hereinafter, a suitable example of the method for producing a fermented product of Lactobacillus herbeticus in the present technology will be described, but the present technology is not limited thereto.

As an example of the method for producing a fermented product of Lactobacillus herveticas in the present technology, it is preferable to include a step of culturing a bacterium belonging to Lactobacillus herveticas in a culture medium. Further, it is preferable to include a step of recovering the culture after the culture as a fermented product.

<1-1-1. Lactobacillus herbeticus of this technology>
Lactobacillus helveticus used in this technique can be used without particular limitation as long as it is a bacterium belonging to Lactobacillus helveticus. Bacteria belonging to the genus Lactobacillus are usually classified as lactic acid bacteria. In this technique, Lactobacillus herbeticus is more preferably a bacterium capable of producing lactic acid.

As the Lactobacillus herbeticus used in this technique, a strain that has been deposited or registered with a predetermined institution may be obtained and used. The prescribed organization is not particularly limited, but for example, the National Institute of Technology and Evaluation Patent Microorganisms Depositary (NPMD, Japan), the National Institute of Technology and Evaluation (NITE) Patent Organism Depositary (IPOD) ( NITE-IPOD (Japan), RIKEN BioResource Center Microbial Materials Development Office (RIKEN BRC-JCM, Japan), American Type Culture Collection (ATCC, USA), etc.
Further, the strain used in this technique is preferably a strain capable of producing a fermented product having an antidepressant effect and / or a feeling of well-being improving effect, has the same mycological properties as this strain, and is substantially the same as the strain. The same strain may be used. The definition of the same or substantially the same can be defined by the same method as the definition of the strain described later.

The Lactobacillus herveticas used in the present technology is not particularly limited, and examples thereof include Lactobacillus herveticas NITE BP-01671.
Of these, it is preferable to use Lactobacillus herveticas NITE BP-01671.
Lactobacillus Helveticas NITE BP-01671 was established on July 29, 2013 by the National Institute of Technology and Evaluation Patent Microorganisms Depositary Center (Room 2-5-8 122, Kazusakamatari, Kisarazu City, Chiba Prefecture, 292-0818). It has been deposited as Lactobacillus herveticus MCC1848 (accession number: NITE BP-01671). This bacterium is generally available from the above preservation institutions.

Lactobacillus herveticas NITE BP-01671 is not limited to the above-mentioned deposit bacterium, and may be a bacterium substantially the same quality as the deposit bacterium. A substantially equivalent bacterium is a bacterium classified into Lactobacillus herbeticus, and the base sequence of its 16S rRNA gene is preferably 99.5% or more, more preferably 99.9% or more of the above-mentioned deposited bacterium. % Or more, more preferably 100%, or strains having the same mycological properties as the deposited bacteria and substantially the same as the deposited strains are also included (Japanese Patent Laid-Open No. 2015-047114). ..
Regarding the strain, "a strain substantially equivalent to the above-mentioned deposit strain" belongs to the same species as the above-mentioned deposit strain, and the effect of this technology (specifically, a fermented product having an antidepressant action or a happiness improving action) is exhibited. Means the strain obtained. The stock substantially equivalent to the deposited stock may be, for example, a derivative stock having the deposited stock as a parent stock. Derivative strains include strains bred from deposit stocks and strains naturally generated from deposit stocks.

Examples of substantially the same strains and derivatives include the following strains.
(1) Probiotics in food / Health and nutritional properties and strains determined to be the same strains as Lactobacillus helveticus NITE BP-01671 by the RAPD method (Randomly Amplified Polymorphic DNA) and PFGE method (Pulsed-field gel electrophoresis). guidelines for evaluation 85 (listed on Page 43)
(2) It has only the gene derived from Lactobacillus herveticas NITE BP-01671 and does not have the gene derived from foreign countries, and its DNA identity with Lactobacillus herveticas NITE BP-01671 is 95% or more (preferably 99%). (3) Lactobacillus herbeticus NITE BP-01671 traits (including strains bred by genetic engineering modification, mutation, or spontaneous mutation) of NITE BP-01671 Owned stock

<1-1-2. Culture medium of this technology>
As the culture medium used in the present technology, generally, a carbon source, a nitrogen source, an inorganic salt, an organic component, an optional component, or the like is appropriately used. In the present technology, for example, the medium normally used for culturing lactic acid bacteria can be appropriately modified as necessary and used as the culture medium of the present technology. The culture medium can be used to grow Lactobacillus herveticas of the present technology and to obtain a fermented product of Lactobacillus herveticas of the present technology.

As the culture medium, for example, as the carbon source, saccharides such as galactose, glucose, fructose, mannose, cellobiose, maltose, lactose, sucrose, trehalose, starch, starch hydrolyzate, and waste sugar honey are used depending on the assimilation property. Can be used. As the nitrogen source, for example, ammonium salts such as ammonia, ammonium sulfate, ammonium chloride and ammonium nitrate and nitrates can be used. Further, as the inorganic salts, for example, sodium chloride, potassium chloride, potassium sulfate, magnesium sulfate, calcium chloride, calcium nitrate, manganese chloride, ferrous sulfate and the like can be used. In addition, organic components such as peptone, soybean flour, defatted soybean meal, meat extract, and yeast extract may be used.

Specific known media include, for example, fortified Clostridia medium (Reinforced Clostridial medium), MRS medium (de Man, Rogosa, and Sharpe medium), mMRS medium (modified MRS medium), TOSP medium (TOS propionate medium), and TOSP. Mup medium (TOS propionate mupirocin medium) and the like can be mentioned. In addition, these known media may be commercially available products and used, or may be appropriately used as a liquid culture.

Further, it is preferable to use a milk component in the culture medium. As a result, fermented milk can be obtained, and the fermented milk is more suitable from the viewpoint of the effect of the present technology (particularly, antidepressant action and happiness improving action).

[1-1-2-1. Milk component]
The milk component is not particularly limited, but for example, milk, buffalo milk, sheep milk, goat milk, horse milk, skim milk, skim milk concentrate, skim milk powder, concentrated milk, whole fat powder milk, cream, butter, butter milk, and the like. Examples include skim milk and milk protein. One or more selected from the group consisting of these can be used. In addition, reduced skim milk may be prepared by appropriately combining these, and the reduced skim milk may be used as a medium.
Moreover, it is preferable to use a milk component derived from milk as the milk component.
The milk protein is not particularly limited, and examples thereof include whey protein, casein protein, and hydrolysates thereof, and one or more selected from the group consisting of these can be used. For the hydrolysis, a general means for hydrolyzing a protein (for example, a protein hydrolase, hydrolysis of an acid or an alkali, etc.) may be used.

The content of the milk component in the culture medium is not particularly limited, but the lower limit is preferably 3% by mass or more, more preferably 8% by mass or more from the viewpoint of the effect of the present technology. Is. The upper limit is preferably 20% by mass or less, more preferably 15% by mass or less, from the viewpoint of cost and fermentation efficiency. The content of the milk component in the medium is even more preferably 8 to 15% by mass.

Further, the culture medium may be blended with probiotics, prebiotics and the like, which will be described later, and the content of the prebiotics is, for example, 0.1 to 1% by mass in the medium.
Here, in general, probiotics refer to bacteria that have a beneficial effect in the intestine. On the other hand, substances that serve as a selective nutrient source for bacteria that have beneficial effects in the intestine and promote their growth are called prebiotics.

<1-1-3. Culture method of this technology>
The culturing method and culturing step in the present technique are not particularly limited, and the method and step usually used for culturing lactic acid bacteria can be appropriately modified and used as necessary. In the culture, a fermented product of the present technology can be obtained.

In the culturing method of the present technology, it is generally possible to use culturing conditions (for example, culturing temperature, culturing time, etc.) in which lactic acid bacteria can be fermented. It is preferable that the culture conditions are such that Lactobacillus herbeticus of the present technology can obtain a fermented product.

It is preferable to use at least Lactobacillus herbeticus of this technology as the "lactic acid bacterium starter" to be inoculated into the culture medium, but other lactic acid bacteria and Bifidobacterium spp. Are combined as long as the effect of this technology is not impaired. You may.

In the culture method of the present technology, the number of bacteria when the lactic acid bacterium starter of the present technology is inoculated into the culture medium is not particularly limited, but is preferably 1 × 10 ³ to 1 × 10 ¹² CFU / g (or) in the culture medium. cells (pieces) / g), more preferably 1 × 10 ⁵ to 1 × 10 ¹¹ CFU / g (or cells (pieces) / g), even more preferably 1 × 10 ⁷ to 1 × 10 ¹⁰ CFU / g (or It is preferable to prepare so as to have cells (pieces) / g).

Further, in the present specification, "CFU" indicates a Colony forming unit. The number of cells (CFU) of the bacteria is the number of colonies that appear when a suspension of appropriately diluted bacteria is spread on a suitable agar medium, for example, a BCP-added plate count agar medium (manufactured by Eiken Chemical Co., Ltd.) and cultured. Can be measured by.

The culture temperature of the present technology is preferably 25 to 45 ° C, more preferably 30 to 42 ° C, and even more preferably 37 to 42 ° C. The culturing time is not particularly limited, but is preferably 2 to 24 hours, more preferably 4 to 18 hours. Further, from the viewpoint of flavor, it is preferable to ferment until the acidity reaches preferably 2%, more preferably 1 to 2%.
Further, the culture is preferably carried out under anaerobic conditions, and for example, the culture can be carried out while aerating an anaerobic gas such as carbon dioxide gas. It can also be cultured under microaerobic conditions by liquid static culture or the like.
Further, as the culture method of the present technology, a continuous method and a batch method may be adopted. Further, a method of culturing the bacteria of the present technology by immobilizing them (for example, a carrier binding method) may be adopted.

Through the culture step as described above, a fermented product of Lactobacillus herbeticus in the present technology can be obtained.
As the fermented product of Lactobacillus herbeticus in the present technology, a culture containing the cells obtained after the culturing step may be used as it is, or a supernatant obtained by removing the cells from the culture may be used. Further, the culture or supernatant may be appropriately diluted or concentrated for use.

When the fermented product of the present technology contains bacterial cells, the bacterial cells may be live or dead, but are preferably dead. Moreover, the bacterial cell may be a crushed product. The crushed product may be a crushed product of live bacteria or a crushed product of dead bacteria, or may be heated or freeze-dried after crushing.

In addition, various steps can be appropriately added after the culturing step as long as the effect of the present technology is not impaired. The various steps are not particularly limited, and examples thereof include a heat sterilization step, a sterilization step, a concentration step, and a drying step, and one or more selected from the group consisting of these can be used.
The method of heat sterilization is not particularly limited, but a conventional heat treatment method or the like can be used. For example, it can be sterilized by heat treatment at 60 to 140 ° C. for 2 seconds to 30 minutes, for example, pasteurization (for example, heat treatment at 60 to 80 ° C. for 5 to 30 minutes) or UHT sterilization (for example, 120 to 140 ° C. 1). ~ 3 seconds) and so on.

The sterilization method is not particularly limited, but a conventional sterilization method or the like can be used. For example, centrifugation, membrane separation and the like can be mentioned. As a result, the cells can be separated and a supernatant can be obtained.
The method of concentration is not particularly limited, but a conventional method of concentration or the like can be used. For example, centrifugation, membrane separation, freeze concentration and the like can be mentioned. As a result, the fermented product can be concentrated by reducing the water content and concentrating the active component and the solid content concentration.
The drying method is not particularly limited, but a conventional drying method or the like can be used. For example, a spray drying method (spray drying method), a freeze drying method and the like can be mentioned.

<2. Applications of fermented products of Lactobacillus herbeticus in this technology>
As shown in Examples below, the fermented product of Lactobacillus herbeticus in the present technology has an antidepressant effect and / or a feeling of well-being improving effect, and symptoms or symptoms that can be prevented, ameliorated, or treated by using the fermented product. It is effective against diseases. Symptoms or illnesses include depression, decreased well-being, depression, dullness, apathy, depression, melancholy, annoyance, gloomy, heavy, unhappy, lack of fulfillment, dissatisfaction, and unsatisfied. One or more species selected from the group consisting of unrewarding, unpleasant, etc. can be mentioned.
Therefore, the antidepressant composition or the happiness-enhancing composition of the present technology contains at least a fermented product of Lactobacillus herbeticus as an active ingredient. That is, the fermented product of Lactobacillus herbeticus in the present technology can be contained and used as an active ingredient in a composition for antidepressant depression and / or for improving happiness.

In addition, the fermented product of Lactobacillus herbeticus in the present technology can be contained in a composition expected to have various effects as an active ingredient, and these various compositions can also be used as a preparation. The fermented product of Lactobacillus herbeticus in the present technology can be used as it is, or can be used as a mixture with a physiologically or pharmaceutically acceptable ordinary simple substance or a diluent. it can.
In addition, the fermented product of Lactobacillus herbeticus in the present technology can be used for various uses such as pharmaceuticals, foods and drinks, and various compositions.

In addition, the present technology can provide a fermented product of Lactobacillus herbeticus, or its use, which is used for the above-mentioned purposes of improving antidepressant or happiness, and preventing / ameliorating depression or decreased happiness.
In addition, the fermented product of Lactobacillus herbeticus in the present technology should be used as the above-mentioned antidepressant method or happiness improving method, the above-mentioned method for preventing / ameliorating / treating the symptoms and diseases, or the active ingredient of these methods. Can be done.
In addition, the fermented product of Lactobacillus herbeticus in the present technology can be used for the production of various preparations or various compositions having the above-mentioned actions or for the above-mentioned purposes of use.

In addition, the present technology can be applied to humans and non-human animals (for example, pets, livestock, etc.). Of these, humans and pets are preferable, and humans are more preferable. In addition, since each active ingredient of this technology has a long eating experience and is known to be highly safe, this technology can be applied to men and women of all ages, but it should be applied to men in this technology. Is preferable.
In addition, the present technology may be used for therapeutic purposes or for non-therapeutic purposes.
"Non-therapeutic purpose" is a concept that does not include medical practice, that is, treatment of the human body by treatment. For example, nutrition and dietary guidance, and maintenance and promotion of health.
"Prevention" refers to the prevention or delay of the onset of a disease or symptom in the application, or the reduction of the risk of the disease or symptom in the application.
"Amelioration" means improvement of a disease, symptom or condition; prevention or delay of exacerbation; reversal, prevention or delay of progression.

This technology can also be used for quasi-drugs, foods with functional claims, foods and drinks, feeds (for example, pets), etc. Examples include quasi-drugs.

<2-1. Form, dose and usage in the composition of the present technology>
The composition containing the fermented product of Lactobacillus herbeticus in the present technology may appropriately contain an arbitrary component as long as the effect of the present technology is not impaired. Examples of the optional component include probiotics, prebiotics, sugars, sugar alcohols, polysaccharides, pH adjusters, fatty acid esters, flavoring agents, fragrances, excipients and the like. At this time, the form of the composition of the present technology is not particularly limited to solid, liquid, semi-solid, powder and the like.

The content or amount of the fermented product of Lactobacillus herbeticus in the present technology can be freely set according to the age, symptoms and the like as long as the effect of the present technology is not impaired. In addition, this amount and usage can be applied to pharmaceutical compositions, food and drink compositions, etc., which will be described later.

The content of the fermented product (dry matter equivalent) of Lactobacillus herbeticus in the present technology can be appropriately blended, and is preferably 10% by mass or more, more preferably 30% by mass or more, still more preferably 30% by mass or more in the composition. It is 50% by mass or more.
In this technique, the amount of the fermented product (dry matter equivalent) of Lactobacillus herbeticus is preferably 0.005 g or more / kg body weight / day, more preferably 0.01 g or more / kg body weight / day. It is more preferably 0.01 to 0.05 g / kg body weight / day.

When the fermented product contains cells of Lactobacillus herbeticus, the content thereof is not particularly limited, but is preferably 1 × 10 ³ to 1 × 10 ¹² CFU / g (or cells (pieces) /). g), more preferably 1 × 10 ⁵ to 1 × 10 ¹¹ CFU / g (or cells (pieces) / g), more preferably 1 × 10 ⁶ to 1 × 10 ¹¹ CFU / g (or cells (pieces) / g). g), more preferably 1 × 10 ⁷ to 1 × 10 ¹⁰ CFU / g (or cells (pieces) / g) may be included in the composition.

The interval of use (specifically, the interval of administration or ingestion) of the fermented product of Lactobacillus herbeticus in the present technique is not particularly limited, and may be once a day or may be divided into a plurality of times. In addition, it is desirable that this technique be used continuously every day (specifically, administered or ingested).
The fermented product of Lactobacillus herbeticus used in this technique is empirically highly safe and can be continuously ingested for a long period of time.

<2-2. Pharmaceutical composition>
The present technology can provide a pharmaceutical composition containing a fermented product of Lactobacillus herbeticus as an active ingredient. The pharmaceutical composition of the present technology can be used as a pharmaceutical composition and can be obtained by a known production method.

The pharmaceutical composition of the present technology is not particularly limited as long as it contains the fermented product of Lactobacillus herbeticus. As the pharmaceutical composition of the present technology, the fermented product of Lactobacillus herbeticus may be used as it is, or a physiologically acceptable liquid or solid preparation carrier may be blended and used as a preparation.

The pharmaceutical composition of the present technology may be for oral use. Since the fermented product of Lactobacillus herbeticus in the present technology is less likely to cause side effects even if it is continuously administered for a long period of time, it can be administered with peace of mind.

The dosage form of the pharmaceutical composition of the present technology is not particularly limited, but in the case of oral administration, for example, solid preparations such as powders, granules, tablets, lozenges, capsules; solutions, syrups, suspensions, emulsions It can be formulated into a liquid preparation such as. In the case of parenteral administration, it can be formulated into, for example, a suppository, a spray, an inhalant, an ointment, a patch, an injection, or the like. In addition, when formulating, the addition of excipients, binders, disintegrants, lubricants, stabilizers, flavoring agents, diluents, surfactants, solvents for injections, etc., which are usually used as formulation carriers. Agents can be used. These components may be appropriately selected by those skilled in the art depending on the dosage form.

The content of the fermented product of Lactobacillus herbeticus in the present technology in the pharmaceutical composition of the present technology is appropriately set according to the dosage form, usage, age and sex of the patient, type of disease, degree of disease, and other conditions. It's okay.

<2-3. Food and beverage composition>
The present technology can also provide food and drink compositions containing a fermented product of Lactobacillus herbeticus as an active ingredient, for example, health foods and functional foods whose concept is to improve antidepressant depression and happiness. Etc. can be provided. The food and drink composition of the present technology can be used as a food and drink composition, and can be obtained by a known production method.

The food and drink composition of the present technology is not particularly limited as long as it contains the fermented product of Lactobacillus herbeticus. Food and beverage compositions include beverages such as soft drinks, carbonated beverages, nutritional beverages, fruit juice beverages, and lactic acid bacteria beverages (including concentrated stock solutions and preparation powders for these beverages); Confectionery such as candy, chewing gum, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream, and baked confectionery; dairy products such as processed milk, dairy beverages, fermented milk, drink yogurt, and butter; Examples include bread; enteral nutritional foods, liquid foods such as porridge, baby foods, baby milk, sports beverages; and other functional foods. In addition, the food and drink composition of the present technology may be a supplement, for example, a tablet-shaped supplement. In the case of supplements, Lactobacillus herbeticus of the present technology can be taken without being affected by other foods in terms of daily dietary amount and calorie intake.
Examples of commercially available foods other than the above include baby food, sprinkle, and Ochazuke seaweed.

The food and drink composition of the present technology can be produced by adding the fermented product of Lactobacillus herbeticus to the raw materials of ordinary food and drink, and other than adding the fermented product, the food and drink composition is the same as that of ordinary food and drink. It can be manufactured in the same manner. The fermented product may be added at any stage of the manufacturing process of the food or drink composition. In addition, a food or drink composition may be produced through a fermentation step using the added Lactobacillus herbeticus of the present technology. Examples of such food and drink compositions include lactic acid bacteria beverages and fermented milk.

In general, "fermented milk" refers to "milk or milk containing non-fat milk solids equal to or higher than this" fermented with lactic acid bacteria or yeast based on the "Ministry Ordinance on Ingredient Standards for Milk and Milk Products". It is defined as "paste-like or liquid or frozen", and its component specifications are "non-fat milk solids content of 8% or more, number of lactic acid bacteria or yeasts (per mL) of 10 million or more, E. coli group negative". It is stipulated.

The types of fermented milk are roughly divided into coagulated yogurt (stationary yogurt, post-fermented yogurt) that is fermented by filling a container with raw materials, and curd that is produced by putting raw materials in a tank and fermenting. There are yogurts (stirring type yogurt, pre-fermentation type yogurt) that are crushed and easily filled with this.

Examples of the stationary yogurt include plain yogurt and hard yogurt.
Examples of the agitated yogurt include soft yogurt, drink yogurt, and frozen yogurt.

[2-3-1. Compositions Containing Probiotics and / or Prebiotics]
In addition, the food and drink composition of the present technology includes components having probiotic effects known or found in the future (for example, lactic acid bacteria and bifidobacteria) or bacteria of the genus Bifidobacterium, as long as the effects of the present technology are not impaired. Ingredients that assist the probiotic effect (eg, prebiotics, etc.) can be used.

For example, the food and drink composition of the present technology includes various proteins such as whey protein, casein protein, soybean protein, or pea protein (pea protein) or mixtures thereof, and decomposition products; leucine, valine, isoleucine, glutamine, and the like. Amino acids; vitamins such as vitamin B6 or vitamin C; creatine; citric acid; fish oil; or prebiotics, isoleucine oligosaccharides, galactooligosaccharides, xylooligosaccharides, soybean oligosaccharides, fructo-oligosaccharides, lactulose, human milk oligos It can be produced by blending components such as oligosaccharides such as sugar (HMO). As the food and drink composition, one kind or two or more kinds selected from the group consisting of these components can be used.

The human milk oligosaccharide is not particularly limited, but is, for example, 2'-fucosyl lactose, 3-fucosyl lactose, 2', 3-difucosyl lactose, lacto-N-triose II, lacto-N-tetraose, lacto-N. -Neotetraose, Lactose-N-Fucospentaose I, Lactose-N-Neofcopentaose, Lactose-N-Fucospentaose II, Lactose-N-Fucospentaose III, Lactose-N-Fucospentaose V, Lact-N-Neofcopentaose V, lacto-N-difucohexaose I, lacto-N-difucohexaose II, 6'-galactosyllactose, 3'-galactosyllactose, lacto-N-hexaose, and lacto-N-neohexaose, etc. Neutral human milk oligosaccharides; acidic human milk oligosaccharides such as 3'-sialyl lactose, 6'-sialyl lactose, 3-fucosyl-3'-sialyl lactose, disialyl-lacto-N-tetraose, etc. One or more selected from the group can be used.

Further, the probiotics are not particularly limited, but probiotics (for example, lactic acid bacteria and / or Bifidobacterium spp.) Used in a conventional method can be used. The amount of probiotics contained in the composition is not particularly limited, but is preferably 1 × 10 ³ to 1 × 10 ¹² CFU / g (or cells (pieces) / g), and more preferably 1 × 10 ⁷ to. It is 1 × 10 ¹⁰ CFU / g (or cells (pieces) / g).

The foods and drinks defined in the present technology can also be provided and sold as foods and drinks labeled for health use.
The "display" act includes all acts for informing the consumer of the use, and if the expression can remind or analogize the use, the purpose of the display, the content of the display, and the display. Regardless of the object or medium to be used, all of them fall under the "display" act of this technology.

Further, it is preferable that the "display" is performed by an expression that allows the consumer to directly recognize the above-mentioned use. Specifically, the act of transferring a product related to food and drink or the packaging of the product with the above-mentioned use, displaying it for delivery, transfer or delivery, and importing it, advertising on the product, price list or transaction documents Examples include the act of describing the above-mentioned use and displaying or distributing it, or describing the above-mentioned use in the information containing these and providing it by an electromagnetic (Internet, etc.) method.

On the other hand, as the display content, it is preferable that the display is approved by the government or the like (for example, a display obtained based on various systems established by the government and performed in a manner based on such approval). In addition, it is preferable to attach such display contents to packaging, containers, catalogs, pamphlets, promotional materials such as POPs at sales sites, and other documents.

In addition, "labeling" includes health foods, functional foods, enteric nutritional foods, special purpose foods, health functional foods, specified health foods, nutritional functional foods, functional foods, non-medicinal products, etc. The display is also mentioned. Among these, in particular, labeling approved by the Consumer Affairs Agency, for example, a system related to foods for specified health use, nutritionally functional foods, or functional foods, or labeling approved by a system similar to these can be mentioned. Specifically, labeling as a food for specified health use, labeling as a conditional food for specified health use, labeling to the effect that it affects the structure and function of the body, labeling for reducing the risk of illness, and functionality based on scientific grounds. Indications, etc. can be mentioned, and more specifically, Cabinet Office Ordinance on permission for special use indications stipulated in the Health Promotion Act (Cabinet Office Ordinance No. 57, August 31, 2001) Labeling as a food for specified health use (particularly labeling for health use) and similar labeling specified in the above are typical examples.

The wording used for displaying as described above is not limited to the wording, and other words may be used for various diseases and symptoms related to the treatment and / or prevention of stress. Needless to say, any wording that expresses the effect of treatment and / or prevention is included in the scope of the present technology. Such words include, for example, "for those who feel depressed", "for those who want to feel satisfied", "for those who want to be happiness", "for those who want to be well-being", "for those who want to feel positive", and "for those who feel better". It is also possible to display based on various uses such as "temporary anxiety, depression, and reduction of confusion" so that consumers can recognize the anti-stress effect.

The food and drink composition of the present technology can be produced by adding the fermented product of Lactobacillus herbeticus in the present technology to the raw material of the food and drink composition.
The food and drink composition of the present technology can be produced by the same method as that of a normal food and drink composition except that the step of adding the fermented product of Lactobacillus herbeticus in the present technology is included.
In the production of the food and drink composition of the present technology, the addition of the fermented product of Lactobacillus herbeticus in the present technology may be carried out at any stage of the production process of the food and drink composition.
Further, in the production of the food and drink composition of the present technology, a step of producing a fermented product of Lactobacillus herbeticus in the present technology may be included. For example, in general, in the process of producing a fermented product of lactic acid bacteria, the fermented product of the present technology may be obtained by adding Lactobacillus herbeticus in the present technology.

In addition, the fermented product of Lactobacillus herbeticus in the present technology can be used in combination with probiotics or the fermented product thereof, depending on the purpose.
In the production of the food and drink composition of the present technology, raw materials and food additives used for ordinary food and drink may be appropriately blended during or after the production process of the food and drink composition.

[2-3-2. Milk powder]
The food and drink composition of the present technology can include milk powder, and can be used particularly for infant formula and mom's milk for mothers during pregnancy and lactation. Infant formula is infant formula for infants 0-12 months old, follow-up milk for infants 6-9 months old and younger (up to 3 years old), at birth Formulated milk powder for low-birth-weight babies weighing less than 2500 g (low-birth-weight babies), various therapeutic milks used to treat children with morbid conditions such as milk allergy and lactose intolerance. Point to. Furthermore, the composition can be applied to foods with health claims and foods for the sick. The health functional food system was established not only for ordinary foods but also for foods in the shape of tablets, capsules, etc., based on internal and external trends and consistency with the conventional food system for specified health use. It consists of two types: foods for specified health use (individual permission type) and foods with nutritional function (standard standard type).

Examples of foods and drinks related to this technology include milk for mothers (prepared milk powder) and dietary supplements for mothers during pregnancy and lactation. Mama's milk refers to milk that contains a well-balanced nutritional requirement for pregnancy and lactation.

Specifically, the milk powder prepared by the present technology may be, for example, milk powder for antidepressant depression or for improving happiness. For example, milk powder for antidepressant depression or for improving happiness can be produced by the following method.

That is, the present technology provides a method for producing milk powder for antidepressant depression or for improving happiness, which comprises the following steps (A) to (C):
(A) A step of culturing Lactobacillus herbeticus bacteria in a medium containing a milk component to obtain a fermented product;
(B) A step of subjecting the fermented product to spray drying and / or freeze-drying to obtain a fermented product powder;
(C) A step of mixing the fermented product powder and prebiotics to obtain milk powder for antidepressant depression or improving happiness.
Here, the milk component may be mixed at the same time as the step (C), before the step (C), or after the step (C).

The present technology also provides a method for producing milk powder for antidepressant depression or for improving happiness, including the following step (A):
(A) A step of mixing prebiotics, a fermented product of Lactobacillus herbeticus, and milk components to obtain milk powder.

[2-3-3. supplement〕
In addition, the food composition of the present technology may be, for example, a supplement for antidepressant depression or for improving happiness. Supplements for antidepressant or happiness enhancement can be produced, for example, by the following methods.

That is, the present technology provides a method for producing a supplement for antidepressant or happiness, which comprises the following steps (A) and (B):
(A) A step of mixing a fermented product of Lactobacillus herbeticus and an excipient to obtain a mixture;
(B) A step of locking the mixture.

In any of the above production methods, components other than the components mentioned in the above steps may be used in combination as appropriate.
The form of the supplement is not particularly limited, and may be in the form of tablets or capsules. In the case of a capsule, for example, the fermented product of Lactobacillus herbeticus or a composition containing the same may be filled in the capsule.

The present technology can also adopt the following configurations.
[1] An antidepressant composition or a happiness-enhancing composition containing a fermented product of Lactobacillus herbeticus of the present technology as an active ingredient.
[2] A method for preventing, ameliorating or treating depression, or a method for improving happiness, in which a fermented product of Lactobacillus herbeticus of the present technology is administered or ingested.
[3] Fermented product of Lactobacillus herbeticus used for antidepressant or happiness improvement, or its use.
[4] Use of a fermented product of Lactobacillus herbeticus for producing an antidepressant composition or a composition for enhancing happiness.
[5] It is preferable that the fermented product is fermented milk. The fermented milk may contain bacterial cells or may have the bacterial cells removed.
Further, it is preferable that the Lactobacillus herveticas is Lactobacillus herveticas NITE BP-01671.
Moreover, it is preferable that the composition is for pharmaceutical use or food and drink.

Hereinafter, the present technology will be described in more detail based on experimental examples, examples, and the like. It should be noted that the experimental examples and examples described below show examples of typical examples of the present technology, and the scope of the present technology is not narrowly interpreted by this.

[Experimental Example 1]
<Manufacturing of test sample>
A fermented milk powder containing Lactobacillus herveticas NITE BP-01671 was produced by the following procedure.
Lactobacillus herveticas NITE BP-01671 strain was inoculated into a medium (sterilized at 90 ° C. for 30 minutes) containing 10% by mass reduced skim milk powder (manufactured by Morinaga Milk Industry Co., Ltd.), cultured at 37 ° C. for 16 hours, and grown. Was used as a lactic acid bacterium starter.
The 10% by mass reduced skim milk powder medium obtained by dissolving skim milk powder in water is sterilized at 90 ° C. for 30 minutes, and seed culture, which is a lactic acid bacterium starter of Lactobacillus herveticas NITE BP-01671, is added (inoculated) to this sterilization medium. )did. At this time, the number of bacteria when the inoculation was added to a approximately 1 × 10 8 ^CFU / g in the culture medium.
After inoculating the lactic acid bacterium starter, it was maintained at a predetermined fermentation temperature and fermented until the lactic acid acidity reached the target value (1.50%) to obtain fermented milk (culture temperature 41 ° C., culture time 8 hours). During production, fermentation was carried out while monitoring each value (Brix value, acidity, pH, etc.) using a sugar content meter, an acidity meter, a pH meter, or the like. Lactobacillus herveticas NITE BP-01671 was contained in the fermented milk in an amount of 1 × 10 ⁹ to 1 × 10 ¹⁰ CFU / g. When the lactic acid bacterium Lactobacillus herveticas NITE BP-01671 is cultured, the medium is usually in an anaerobic state.
The obtained fermented milk was spray-dried with a spray dryer to obtain a fermented milk powder to be used as a test food. Lactobacillus herveticas NITE BP-01671 was contained in the fermented milk powder in an amount of 1 × 10 ⁹ to 1 × 10 ¹⁰ CFU / g.
In the following examples, 1.2 grams of fermented milk powder per day (containing about 10 billion killed lactic acid bacteria) was encapsulated and used as a test sample. The amount of this fermented milk powder used is about 0.01 to 0.05 g / 60 kg body weight / day in terms of dry matter. In addition, the fermented milk powder is 95% by mass or more in the filled capsule.

<Subject>
We recruited adult men and women between the ages of 20 and 60 at the time of consent acquisition as subjects, and registered those who had a feeling of depression or anxiety in clinical trials from a simple psychological questionnaire conducted at the time of recruiting the WEB. did. Furthermore, according to the subject background survey, 80 subjects who did not violate the following exclusion criteria were selected as subjects.

(1) Depression or those who have been diagnosed with depression in the past (2) Those who are being treated for chronic illness (3) Those who have a desire to commit suicide (4) Those who have food allergies or hypersensitivity to the test food ingredients Those who have a history (5) Those who are pregnant, breastfeeding, or willing to become pregnant during the test period (6) Those who have participated in other drug or food tests within the past month (7) Intestinal flora Those who regularly use medicines and supplements that affect them (8) Those who fall under the category of heavy drinkers (9) Those who are judged by the investigator or the investigator to be inappropriate as subjects

<Test method>
The subjects underwent a psychological test, which is an index of depressed mood and anxiety, before ingesting the test sample, and were divided into two groups of 40 subjects each using the psychological test score as a stratification factor. Next, the subjects in each group were distributed a test sample or a placebo sample indistinguishable from the test sample, and the test sample or placebo sample was ingested once a day for 8 consecutive weeks regardless of morning, noon, or night. A psychological test was performed again after the end of ingestion to evaluate the change from the score before ingestion.
One of the subjects was unable to participate in the test at the end of intake (at 8 weeks) and data was lost, and the other one had a low intake rate of the test food of less than 70%, so from the study protocol. Since it was considered to be a deviation, it was excluded from the analysis. Therefore, in this study, the analysis was performed on 78 subjects who adhered to the study plan and completed the study.

For the psychological test, the State-Trait Anxiety Initiative (STAI state-characteristic anxiety scale), which is a psychological test for measuring depressed mood and anxiety, was performed. The STAI test is divided into characteristic anxiety and state anxiety, and each has 20 questions. The question items are shown below.
Ask the subject to perform from FORM X-1. Regarding FORM X-1, teach "Please mark one place in the column that best applies to your current feelings (" Totally different 1 "," Somewhat 2 "," Okay 3 ","That's right 4 ")." And ask the subject to do it.
Next, move on to FORM X-2 and have the subject perform FORM X-2. Instruct the subject about FORM X-2, "Please mark one of the columns that best apply to your feelings (" Almost none 1 "," Sometimes 2 "," Frequently 3 "," Frequently 4 ")." Have it implemented.
The psychological test (specifically, implementation method and arrangement method (paper, scoring method, etc.), judgment method, etc.) of (STAI state-characteristic anxiety scale) performed in this experimental example is "Japanese version of STAI (State-". Trait Anxiety Industry) Form X User's Guide (enhanced version); Original author Spielberger, CD / Organizer Kiminobu Mizuguchi, Junko Shimonaka, Katsuji Nakazato; Published in 2012; Sankyobo Co., Ltd. ..

"State anxiety" is said to be "anxiety depending on the situation." For example, there are scenes where a scary dog is barking just before an important test or near, but just before or in such a scene, everyone gets nervous and anxious. It can be said that anxiety about the condition is increasing at such times.
"Characteristic anxiety" is said to mean "a person's personality is usually evasive to danger or easy to worry about." Moreover, since this characteristic does not change depending on the state, it is said that this characteristic does not change much after formation.

Regarding the results of the psychological test, the amount of change before and after ingestion was calculated, and the significant difference between the groups was performed using the Wilcoxon rank sum test.

<Result>
In the test sample intake group, a significant difference was observed between the groups in the items shown in Table 3 below as compared with the placebo sample intake group.

The STAI score is an index for evaluating that the larger the value, the worse the anxiety symptom associated with each item.
Of the items of "STAI state anxiety" (20 items) and "STAI characteristic anxiety" (20 items), items other than these two items of "become depressed" and "feel satisfied" of STAI characteristic anxiety , Good results were not obtained.
As shown in Table 3, the STAI score decreased (improved) significantly in the test sample intake group compared with the placebo group, so that the "depressed" emotional change was improved and "satisfied mood", that is, It was suggested that "happiness" could be obtained.
Regarding men, as a result of STAI characteristic anxiety, the P values of "being depressed" and "being satisfied" are 0.08 and 0.03, respectively, and the effect is better for men. Was seen.

As shown in the item of "STAI score", there are various specific contents of "anxiety". For this reason, there are users and markets who desire to exert their effects only on specific items as much as possible, while exerting little effect on other items. In modern society, a composition having an anti-depressive effect and an effect of improving happiness is particularly desired, and it is also desired that this composition is highly safe even if it is ingested daily.
In Example 1 using the fermented product of the present technology, effective effects were obtained in the two items of item numbers 35 and 36, "become depressed" and "become satisfied", while other than these two items. No effective effect was obtained with 38 items. That is, the present inventor was able to find a substance more specialized in "anti-depressive action" and "happiness improving action", that is, a fermented product of Lactobacillus herbeticus of the present technology.
Therefore, it has become possible to provide a composition specialized for antidepressant depression or improvement of happiness by using a highly safe fermented product of Lactobacillus herbeticus.

<Prescription example>
Examples of production and prescription of the food and drink composition of the present technology are shown below, but the composition of the present technology is not limited to this.

[Prescription example 1: Drink yogurt]
Reduced skim milk powder (manufactured by Morinaga Milk Industry Co., Ltd.) was dissolved in water to obtain a 10% by mass reduced skim milk powder medium. 1000 mL of this 10 mass% reduced defatted milk powder medium is sterilized at 90 ° C. for 30 minutes, and 30 mL of seed culture of Lactobacillus herveticas NITE BP-01671 strain is inoculated into this sterilized medium, cultured at 37 ° C. for 16 hours and grown, and lactic acid bacteria Got a starter.
Separately from this, 0.5% by mass of milk fat, 8.0% by mass of non-fat milk solids, and sucrose obtained by mixing and dissolving raw materials containing skim milk powder, whole milk powder, pectin, and sucrose. 50 L of base medium containing 0% by mass and 0.2% by mass of pectin was sterilized at 90 ° C. for 10 minutes and cooled to 40 ° C.
The sterilized base medium was inoculated with 50 mL of the lactic acid bacterium starter of the above-mentioned Lactobacillus herveticas NITE BP-01671 strain, and cultured at 37 ° C. for 7 hours under anaerobic conditions to obtain fermented milk.
The fermented milk was immediately stirred and cooled, and the cooled fermented milk was homogenized at a pressure of 15 MPa, filled in a 200 mL glass container and sealed to produce drink yogurt. Lactobacillus herbeticus NITE BP-01671 was ^{contained in the drink yogurt at 1 × 10 9} to 1 × 10 ¹⁰ CFU / g.

Since the produced drink yogurt is a fermented product of Lactobacillus herveticas, this drink yogurt can be used as an antidepressant drink yogurt or a drink yogurt for improving happiness, which contains a fermented product of Lactobacillus herveticas as an active ingredient. ..

[Prescription example 2: Fermented milk]
1000 mL of 10 mass% reduced skim milk powder medium was sterilized at 90 ° C. for 30 minutes, 30 mL of seed culture of Lactobacillus herveticas NITE BP-01671 strain was inoculated, and the mixture was cultured at 37 ° C. for 16 hours and grown to obtain a lactic acid bacterium starter. A lactic acid bacterium starter with a mixed culture of Streptococcus thermophilus and Lactobacillus bulgaricus was also obtained using the same culture method.
Separately, 50 L of raw milk containing 3.0% by mass of milk fat and 9.0% by mass of non-fat milk solids is heated to 70 ° C., homogenized at a pressure of 15 MPa, and then sterilized at 90 ° C. for 10 minutes. Then, the mixture was cooled to 40 ° C. to obtain sterilized milk. This pasteurized milk is inoculated with 500 mL of the above-mentioned lactic acid bacterium starter of the Lactobacillus herveticas NITE BP-01671 strain and 5 mL of a mixed culture of Streptococcus thermophilus and Lactobacillus bulgaricus, filled in a 500 mL resin container, and sealed. Then, under anaerobic conditions, the cells were cultured at 37 ° C. for 7 hours and then immediately cooled to produce fermented milk.

Since the fermented milk produced is a fermented product of Lactobacillus herveticas, this fermented milk can be used as a fermented milk for antidepressant depression or a fermented milk for improving happiness, which contains a fermented product of Lactobacillus herveticas as an active ingredient. ..

[Prescription Example 3: Prepared milk powder and supplements]
The fermented milk powder obtained in Example 1 can be mixed with human milk oligosaccharides containing 2'-fucosyl lactose and / or 3'-sialyl lactose to obtain prepared milk powder.
In addition, a tablet-type supplement can be obtained by tableting a mixture of the prepared milk powder and an excipient.
This formula can be used as a formula for antidepressant depression or a formula for improving happiness, which contains a fermented product of Lactobacillus herbeticus as an active ingredient.

In addition, this supplement can be used as an antidepressant supplement or a happiness-enhancing supplement containing a fermented product of Lactobacillus herbeticus as an active ingredient.
In addition, the cells of Lactobacillus herbeticus are removed from the fermented milk in Example 1 to produce a supernatant. It is also possible to obtain a formula for depression or a formula for improving happiness, a supplement for antidepressant or a supplement for improving happiness by using the supernatant containing no Lactobacillus herveticas cells.

Claims (4)

An antidepressant composition or a happiness-enhancing composition containing a fermented product of Lactobacillus herbeticus as an active ingredient. The composition according to claim 1, wherein the fermented product is fermented milk. The composition according to claim 1 or 2, wherein the Lactobacillus herveticas is Lactobacillus herveticas NITE BP-01671. The composition according to any one of claims 1 to 3, wherein the composition is a food and drink composition.