JP2021023149A - Method for supplying cell-containing liquid, device for supplying cell-containing liquid, and method for manufacturing cell-containing product - Google Patents

Abstract

To provide a method for supplying a cell-containing liquid that is capable of filling a certain amount of a cell-containing liquid in a container in a short time and is less likely to affect the qualities of cells, such as homogeneity and viability, between multiple containers.SOLUTION: For a combination of a main pile 22, which is a series of pipelines; a plurality of branch pipes 23, which are pipelines branched laterally from the main pipe 22; and a plurality of containers B connected to the plurality of branch pipes 23, the main pipe 22 is filled with a cell-containing liquid L in which living cells are dispersed in a state where the plurality of branch tubes 23 are closed. After that, by opening the plurality of branch pipes 23, the containers B are supplied from the main pipe 22 through the respective branch pipes 23.SELECTED DRAWING: Figure 1

Description

The present invention relates to a method for supplying a cell-containing liquid, a device for supplying the cell-containing liquid, and a method for producing a cell-containing product when supplying a liquid containing viable cells to a container.

In recent years, it has been put into practical use that cells are separated and / or cell culture is performed using tissues, cells, fertilized eggs and the like of various parts of the human body, and the prepared cells are used for regenerative medicine. The prepared cells are transported from the place of preparation to a place of use such as a medical institution or a research institution. At this time, it is conceivable that the container is filled with a liquid containing viable cells to form a cell-containing product. If the cell-containing product is packed in a container, it can be easily put on the distribution route. Further, by filling the container, it is possible to maintain the quality of cells and the like.

Here, for example, Patent Document 1 describes a method of filling a container with a liquid related to a biological sample. In this method, the bottoms of the respective storage chambers of a plurality of containers are set to substantially the same height, and the liquids are connected so as to flow through the respective storage chambers in order to be fed.

JP-A-2018-126195

In this method, the liquid is supplied to the container with a time lag from the container on the upstream side to the container on the downstream side. For this reason, filling of different containers may affect quality such as cell homogeneity and viability by changing the time.

Therefore, according to the present invention, a method for supplying a cell-containing liquid, which can fill a container with a certain amount of the cell-containing liquid in a short time and does not easily affect the quality such as cell homogeneity and viability, a cell-containing liquid supply device, An object of the present invention is to provide a method for producing a cell-containing product.

The present invention relates to a combination of a main pipe, which is a series of pipelines, a plurality of branch pipes, which are pipelines branched laterally from the main pipe, and a plurality of containers connected to the plurality of branch pipes. The cell-containing liquid in which the living cells are dispersed is filled in the main pipe in a state where the plurality of branch pipes are closed, and then the plurality of branch pipes are opened, so that the main pipe is used via each branch pipe. This is a method of supplying a cell-containing liquid to be supplied to each container.

Further, the present invention is a method for producing a cell-containing product in which a container is filled with a cell-containing solution in which viable cells are dispersed, and the main is a series of pipelines and the main is branched laterally from the main. The main pipe is filled with a cell-containing liquid in a state where the plurality of branch pipes are closed for a combination of the plurality of branch pipes which are the pipelines and the plurality of containers connected to the plurality of branch pipes. After that, by opening the plurality of branch pipes, the cell-containing product is supplied from the main pipe to each container via each branch pipe.

According to these methods, by filling the main pipe with the cell-containing liquid and then supplying the cell-containing liquid to each container via each branch pipe, the cell-containing liquid can be supplied to a plurality of containers at the same time, so that the supply conditions can be made uniform. it can.

Further, the container is a container that expands with the supply of the cell-containing liquid, and the container has an entrance / exit portion for taking in / out the cell-containing liquid, and the peripheral portion other than the portion where the entrance / exit portion is formed. Is liquid-tight and airtightly closed, and after the cell-containing liquid is supplied to the container through the inlet / outlet portion, the air inside the container may be discharged from the inlet / outlet portion. ..

According to this, after the cell-containing liquid is supplied to the container, the air inside the container is discharged from the inlet / outlet portion, so that the air remaining after filling can be prevented from adversely affecting the cell-containing liquid.

Further, the present invention is a cell-containing liquid supply device for putting a cell-containing liquid in which viable cells are dispersed into a container, and is connected to a storage portion for storing the cell-containing liquid and the storage portion. A main line, which is a series of pipelines, a plurality of branch pipes, which are pipelines branched laterally from the main line, and a liquid feed pump that supplies cell-containing liquid from the storage unit to the main line. A plurality of on-off valves provided in the plurality of branch pipes and a control unit are provided, and the control unit closes the plurality of on-off valves and then operates the liquid feed pump to release cell-containing liquid. After supplying the cell-containing liquid from the storage unit to the main pipe and filling the main pipe with the cell-containing liquid, the plurality of on-off valves are opened, and the cell-containing liquid is supplied from the main pipe to each container via each branch pipe. It is a supply device for a cell-containing liquid to be supplied.

According to this configuration, by filling the main pipe with the cell-containing liquid and then supplying the cell-containing liquid to each container via each branch pipe, the cell-containing liquid can be supplied to a plurality of containers at the same time, so that the supply conditions can be made uniform. ..

Further, the main body is provided with a liquid recovery pump provided on the side opposite to the side to which the storage portion is connected, and the container is a container that expands with the supply of the cell-containing liquid. Has an entrance / exit portion for taking in and out a cell-containing liquid, and is closed in a liquid-tight and airtight manner except for a portion of the peripheral portion where the entrance / exit portion is formed, and the control unit is a cell via the entrance / exit portion. By operating the liquid recovery pump after the contained liquid is supplied to the container, the air inside the container may be discharged from the inlet / outlet portion of the container.

According to this configuration, after the cell-containing liquid is supplied to the container, the air inside the container is discharged from the inlet / outlet portion, so that the air remaining after filling can be prevented from adversely affecting the cell-containing liquid. ..

According to the present invention, the conditions for supplying the cell-containing liquid to a plurality of containers can be made uniform. Therefore, a certain amount of cell-containing liquid can be filled in a container in a short time, and the quality such as cell homogeneity and viability between a plurality of containers is not easily affected. It is possible to provide a liquid supply device and a method for producing a cell-containing product.

It is a figure which shows schematic structure of the manufacturing apparatus of the cell-containing product which concerns on one Embodiment of this invention. It is the figure which looked at the main part of the manufacturing apparatus from the side of a container. It is a view of the arrow III-III in FIG. It is a block diagram of the manufacturing apparatus.

Hereinafter, the cell-containing product manufacturing apparatus 1 (hereinafter, simply referred to as “manufacturing apparatus”) according to the embodiment of the present invention will be described, and a method for producing the cell-containing product will also be described. The cell-containing product is a container B filled with a cell-containing solution in which living cells are dispersed. The manufacturing apparatus 1 supplies the cell-containing liquid L in which the living cells are dispersed to the container B which is the outer cover of the cell-containing product with respect to the cells prepared outside the manufacturing apparatus 1, and the cell-containing liquid L It is a device for producing a cell-containing product filled with. In this device, dispensing is performed by supplying a specified amount of cell-containing liquid L to each of a plurality of containers. This manufacturing apparatus 1 is also a filling apparatus when focusing on the filling of the cell-containing liquid L.

The manufacturing apparatus 1 of the present embodiment includes a liquid supply unit 2, a container arrangement unit 3, and a control unit 4 (see FIG. 4). The liquid supply unit 2 is a portion that supplies the cell-containing liquid L toward the container B, and is mainly a storage unit 21, a main pipe 22, a branch pipe 23, a liquid feed pump 24, a liquid recovery pump 25, and a main on-off valve. (Upstream valve 26, downstream valve 27), and a plurality of branch pipe on-off valves 28 ... 28 are provided. The control unit 4 controls each unit in the manufacturing apparatus 1.

The storage unit 21 has a container (tank) 211 for storing the cell-containing liquid L before being supplied to the container B. The container 211 of the present embodiment has an inlet for containing the cell-containing liquid L, and this inlet is closed by a lid 212 that can be opened and closed. For example, the cell-containing liquid L may be directly introduced from the pipe connected to the storage unit 21, and in this case, an inlet for the cell-containing liquid L is not required. Further, the storage unit 21 has a stirring unit 213 inside the container 211. The stirring unit 213 has, for example, rotating blades and stirs the cell-containing liquid L in order to maintain the dispersed state of the living cells. Further, although not shown, the storage unit 21 has a temperature adjusting unit. The temperature adjusting unit is a cooling jacket or a cooling plate provided so as to come into contact with the storage unit 21, and maintains the cell-containing liquid L at a constant temperature (low temperature). Therefore, the cell-containing liquid L is sent out toward the container B in a cooled state (4 ° C. in this embodiment). Although the temperature adjusting unit in the storage unit 21 has been mentioned here, it is also possible to provide an overall temperature adjusting unit (not shown) that adjusts the overall temperature of the manufacturing apparatus 1, thereby containing moving cells. The liquid L is also desirable because it is maintained at a constant temperature (low temperature). The storage unit 21 of the present embodiment includes a spinner flask as a container 211 and a table on which the spinner flask is placed, and includes a cooling table as a temperature control unit.

The main pipe 22 is a series of pipes and is connected to the storage unit 21. In this embodiment, a resin tube (specifically, a soft resin) is used. As shown in FIG. 2, the main 22 is formed in a series by connecting a plurality of tubes with connecting members. In the present embodiment, as shown in FIG. 1, three main pipes 22 are provided in parallel with branch pipes 23, and three main pipes 22 are provided on the upstream side and the downstream side of each main pipe 22. Are integrated into one to form an aggregation pipe 22A. Further, the number of three is only an example, and the number can be variously determined according to the specifications of the manufacturing apparatus 1. In the main pipe 22 of the present embodiment, both the upstream side 221 and the downstream side 222, which are the centralized pipe 22A, are connected to the storage section 21, and form a circulation path as shown in FIG. The circulation path is advantageous because the surplus cell-containing liquid L after the filling of the container B is completed can be recovered in the storage unit 21. When the overall temperature adjusting unit is provided as described above, the surplus cell-containing liquid L is also maintained at a constant temperature (low temperature), so that the cell-containing liquid L can be reused.

The branch pipe 23 is a pipe line that branches laterally from the main pipe 22 and is connected to the container B. In this embodiment, a resin tube (specifically, a soft resin) is used. A plurality of branch pipes 23 ... 23 branch from the main pipe 22. The plurality of branch pipes 23 ... 23 have the same inner diameter dimension and length. In the present embodiment, 10 branch pipes 23 are branched from each of the three main pipes 22 in the parallel portion shown in FIG. 1 as shown in FIG. The number of 10 is only an example, and the number of branches of the branch pipe 23 can be variously determined according to the specifications of the manufacturing apparatus 1.

The main pipe 22 and the plurality of branch pipes 23 ... 23 can be combined to form a set of assemblies. By assembling in this way, the main pipe 22 and the branch pipe 23 are replaced after the first filling work is completed and before the second filling work is performed (replacement is in principle every time of the filling work). Since this can be done by exchanging the entire set of assemblies, the work load of connecting the pipes can be reduced.

Here, the container B will be described. The container B used in the present embodiment has the same structure as a known infusion bag (drip bag, etc.), and is liquidtight and liquid-tight due to the peripheral portions of two flexible resin films facing each other being adhered to each other. It is airtightly closed. Therefore, the container B is an expandable container and is a flexible bag that expands with the supply of the cell-containing liquid L. This bag shrinks when bleeding air, as described below. That is, the container B has a bag shape and its volume changes. From this configuration, there is a correlation between the facing distance between the two resin films and the amount of liquid filled inside. The planar shape of the container B is not particularly limited, but is often rectangular (substantially rectangular) or oval. The container B of the present embodiment has a substantially rectangular shape as shown in FIG. 3, and is provided with a plurality of (three places in the present embodiment) mouth portions on one side (upper side in the arranged state at the time of filling). ing. In the present embodiment, two of the three locations (the mouth portion on the left side and the mouth portion on the substantially central side in FIG. 3) are closed by the plug B1. The other one is used as the entrance / exit portion B2, and is used for taking in / out the cell-containing liquid L when filling. The entrance / exit portion B2 is located on the end side (right side in FIG. 3) of the center on one side of the container B. By the way, a groove B11 is formed in the circumferential direction on the outer peripheral portion of the plug B1. As shown in FIGS. 2 and 3, the groove B11 is hooked on a pair of bag locking portions 34 formed from a pair of rods arranged opposite to each other in the container arrangement portion 3, so that the groove B11 is hooked on the container arrangement portion 3. Can position the container B. Therefore, the groove B11 has a shape corresponding to the bag locking portion 34. As shown in FIG. 3, the entrance / exit tube B21 is integrally provided in the entrance / exit portion B2. The entrance / exit tube B21 is closed and cut at an intermediate position after the filling of the cell-containing liquid L is completed.

The liquid feed pump 24 functions as an extrusion pump, pressurizes the cell-containing liquid L, and supplies it from the storage unit 21 to the main 22. The liquid recovery pump 25 functions as a suction pump, and by sucking the cell-containing liquid L in the main pipe 22, the surplus cell-containing liquid L after filling the container B is collected and returned to the storage unit 21. Further, the liquid recovery pump 25 discharges the cell-containing liquid L from the container B by sucking the air inside the container B together with the excess cell-containing liquid L after the cell-containing liquid L is supplied to the container B.

As the liquid feed pump 24 and the liquid recovery pump 25, a known tube pump that feeds liquid by squeezing the outside of the tube (the tube constituting the main pipe 22 in this embodiment) with a pressing means such as a roller is used. .. Therefore, since the cell-containing liquid L does not come into direct contact with the inside of each pump, the cell-containing liquid L is not contaminated in the pumps 24 and 25.

The main pipe on-off valve is provided on the upstream side and the downstream side of the branch positions of the plurality of branch pipes 23 ... 23 in the main pipe 22, and can open and close the main pipe 22. The main on-off valve is composed of an upstream valve 26 provided on the upstream side of the main 22 and a downstream valve 27 provided on the downstream side of the main 22. Each branch pipe opening / closing valve 28 is provided in each branch pipe 23, and each branch pipe 23 can be opened / closed. By combining the opening and closing of each on-off valve 26 to 28 and the selective operation of the liquid feed pump 24 and the liquid recovery pump 25, the cell-containing liquid L is filled into the main pipe 22 via the main pipe 22 and the branch pipe 23. The cell-containing liquid L can be supplied to the container B, and the air in the container B can be sequentially bleeded through the branch pipe 23 and the main pipe 22. As each on-off valve 26 to 28, a known pinch valve that closes by crushing the flow path space in the tube by pressing from the outside of the tube by an actuator or the like is used. Therefore, the tubes included in the main pipe 22 and the branch pipe 23 are made of a flexible material that is deformed by external pressure.

The container arrangement portion 3 is a portion that supports a plurality of containers B ... B at least from the start of supply of the cell-containing liquid L to the end of supply. As shown in FIG. 2, a plurality of block-shaped side members 31 arranged at regular intervals in the horizontal direction are provided so as to extend in the horizontal direction and abut on the lower ends of the plurality of side members 31. The flat plate-shaped bottom member 32 is provided. As shown in FIG. 3, the side member 31 and the bottom member 32 are provided so as to be inclined in a lateral view (a plan view based on the container B reference). The side member 31 and the bottom member 32 are fixed to the flat plate-shaped rear member 33. By this fixing, the two adjacent side members 31 are kept at a constant distance. The container arranging portion 3 is provided with a pair of bag locking portions 34 above the pair of adjacent side members 31. The bag locking portion 34 locks a part of the container B, and is a rod-shaped body (specifically, a round bar) protruding from the rear member 33 in the present embodiment. By hooking the stopper of the container B on the pair of bag locking portions 34, the container B is positioned with respect to the container arranging portion 3. When the container B is arranged in the container arrangement portion 3, the container B is in contact with the bottom member 32 and the side member 31. However, the container B may be arranged so as not to come into contact with the bottom member 32.

A pair of facing wall surfaces 31A and 31A are formed by the two adjacent side members 31. Each wall surface 31A is a vertical plane, and the pair of wall surfaces 31A and 31A face each other in parallel. A container B before being filled with the cell-containing liquid L is arranged between the pair of wall surfaces 31A and 31A. Each wall surface 31A formed by the side member 31 has a region in the container B that freely expands with the supply of the cell-containing liquid L (that is, two flexible resin films are adhered to the peripheral portion of the container B). As a result, free expansion is restricted, so that the region is provided to the outside of the region (excluding the portion where the amount of expansion is smaller than that of other portions).

A bottom surface 32A that supports the container B from below is formed below the pair of wall surfaces 31A and 31A that face each other. The bottom surface 32A corresponds to the top surface of the bottom member 32. Since the bottom member 32 is inclined, one (right side in FIG. 3) of the bottom surface 32A is high and the other (left side) is low when focusing on the line intersecting the pair of wall surfaces 31A and 31A. The surface is inclined as if it were.

When the cell-containing liquid L is supplied to the plurality of containers B ... B in the container arrangement portion 3, each container B expands. However, since the container B is sandwiched between the pair of wall surfaces 31A and 31A, the container B is further moved after the resin film constituting the container B is brought into close contact with each wall surface 31A, particularly in the "freely expanding region". Does not expand. That is, the pair of wall surfaces 31A and 31A regulates the expansion of the space volume inside the bag at that time. Correspondingly, the container B is filled with the cell-containing liquid L having a volume corresponding to the facing distance between the pair of wall surfaces 31A and 31A (and no further filling is performed).

Next, the steps of the method for producing the cell-containing product (method for filling the cell-containing liquid L) according to the present embodiment will be described. First, a plurality of containers B (10 in the example shown in FIG. 2) are arranged so as to be inserted between the pair of opposite wall surfaces 31A and 31A of the container arrangement unit 3. At this time, the container B is inclined and arranged between the pair of wall surfaces 31A and 31A so that the entrance / exit portion B2 is located at the upper end (specifically, the upper end in the space inside the bag). At the same time, the cell-containing liquid L is placed in the container 211 of the storage unit 21. Further, after the main pipe 22 is attached to the liquid feeding pump 24 and the liquid recovery pump 25, the upstream side and downstream side centralizing pipes 22A of the main pipe 22 are connected to the storage unit 21. Further, the container B is connected to each branch pipe 23. By this connection, the main 22 having the tube and the set of each branch pipe 23 are connected to the storage portion 21 and the container B. Further, this set is also connected to the liquid recovery pump 25. In addition, each on-off valve 26 to 28 is set so as to be able to operate.

Next, the liquid recovery pump 25 is operated after the upstream valve 26 of the main on-off valve is closed, the downstream valve 27 is opened, and all the branch on-off valves 28 ... 28 are opened. As a result, preliminary air bleeding, which is the work of bleeding air from the container B before filling, is performed for a certain period of time. After a lapse of a predetermined time, all the branch on-off valves 28 ... 28 are closed.

Here, the "predetermined time" is a fixed time set in advance, and is set corresponding to a time in which each operation (here, preliminary air bleeding) is expected to be achieved (hereinafter, hereafter. Same for other operations). In this way, the control unit 4 of the present embodiment controls each on-off valve 26 to 28 or stops the supply of the cell-containing liquid L in the so-called "expected time" without detecting the filling status in real time. I am doing. As described above, in the present embodiment, the filling control can be performed under relatively loose conditions as compared with the control in which the filling status is detected every moment and the supply amount of the cell-containing liquid L is fed back. It is advantageous because the design cost related to the above can be reduced.

Next, the upstream valve 26 of the main on-off valve is opened, and the liquid feed pump 24 and the liquid recovery pump 25 are operated. As a result, the cell-containing liquid L of the reservoir 21 is sent to the empty main 22, and the inside of the main 22 is filled with the cell-containing liquid L. As a result, the cell-containing liquid L is filled inside the main 22 (particularly, the portion of the main 22 where the plurality of branch pipes 23 ... 23 are branched). After a lapse of a predetermined time, the liquid feed pump 24 and the liquid recovery pump 25 are stopped, and the downstream valve 27 of the main on-off valves is closed.

Next, all the branch pipe on-off valves 28 ... 28 are opened, and the liquid feed pump 24 is operated. All the branch on-off valves 28 ... 28 are opened at the same time (simultaneously) by the control unit 4. As a result, the cell-containing liquid L that was first filled in the main pipe 22 and then the cell-containing liquid L that was sent from the storage unit 21 via the main pipe 22 are supplied to the branch pipe 23. In this way, the cell-containing liquid L is supplied to the container B via the branch pipe 23 and the entrance / exit portion B2, the container B is expanded, and the cell-containing liquid L having a volume corresponding to the facing distance between the pair of wall surfaces 31A and 31A. Is filled in the container B. Since the expansion of the container B is regulated by the pair of wall surfaces 31A and 31A, after the container B is filled with the specified amount of the cell-containing liquid L (that is, after the container B is in close contact with the pair of wall surfaces 31A and 31A). ), Since there is no space for receiving the cell-containing liquid L in each container B, the cell-containing liquid L cannot enter each container B. Since the downstream valve 27 is closed at this point, the cell-containing liquid L that does not enter each container B stays in the main pipe 22 (of which, the upstream side of the downstream valve 27) and the branch pipe 23.

In the present embodiment, the cell-containing liquid L having a volume corresponding to the facing distance between the pair of wall surfaces 31A and 31A can be filled in the container B with the supply (that is, in the flow of the supply), so that the supply rate can be adjusted during the supply. No need to change. Further, in the present embodiment, the cell-containing liquid L is filled in the main pipe 22 and then supplied to each container B via each branch pipe 23, whereby the cell-containing liquid L is supplied to the plurality of containers B ... B at the same time. Therefore, the supply conditions can be made uniform. Therefore, depending on the supply status of the cell-containing liquid L, it is difficult to affect the quality such as cell homogeneity and survival rate between a plurality of containers B ... B, and a high-quality product having high medicinal and pharmacological effects is supplied. it can.

When supplying the cell-containing liquid L, in order to surely fill each container B with the specified amount of the cell-containing liquid L (to prevent insufficient filling), the amount of the cell-containing liquid L larger than the specified amount is received from the storage unit 21. Is supplied. After a lapse of a predetermined time, the liquid feed pump 24 is stopped, and the upstream valve 26 of the main main on-off valve and all the branch on-off valves 28 ... 28 are closed.

Next, the liquid recovery pump 25 is operated after opening the downstream valve 27 of the main on-off valve. At this time, of the plurality of branch pipe on-off valves 28 ... 28, for example, only one on the most upstream side is opened. As a result, the container B corresponding to the opened branch pipe 23 is evacuated to discharge the air inside from the inlet / outlet portion B2. By bleeding air, it is possible to prevent the air remaining in the container B after filling from adversely affecting the cell-containing liquid L. After a lapse of a predetermined time, one of the plurality of branch pipe on-off valves 28 ... 28 that had been opened was closed, and only the other one was opened, and air was bleeded from the container B corresponding to the opened branch pipe 23. Will be done. This is also sequentially performed in the other branch pipes 23. If this air bleeding is performed in a plurality of containers B ... B at the same time, there will be a container B in which a large amount of air and the cell-containing liquid L are sucked out and a container B in which the sucking out is limited to a small amount, resulting in variation. Therefore, it is carried out sequentially in each container B. After a predetermined time has elapsed for the final air bleeding, the liquid recovery pump 25 is stopped, and the downstream valve 27 of the main on-off valves is closed.

After that, the container B is taken out from the container arranging portion 3, and the inlet / outlet tube B21 integrally provided at the inlet / outlet portion B2 of the container B is closed by welding or the like and then cut. As a result, a cell-containing product is completed. The finished cell-containing product is frozen as needed, put on the market, and the cells in the cell-containing product are used.

As described above, according to the present embodiment, it is not necessary to change the supply rate during supply. Therefore, although it has a simple structure, a certain amount of cell-containing liquid L can be filled in the container B in a short time, and the quality such as cell homogeneity and survival rate is not easily affected, and moreover, medicinal effect and pharmacological effect. We can supply high quality products.

Although one embodiment of the present invention has been described above, the present invention is not limited to the above-described embodiment, and various modifications can be made without departing from the gist of the present invention.

The main 22 constitutes a circulation path in the above embodiment. However, the present invention is not limited to this, and for example, the cell-containing liquid L discharged from the storage unit 21 may be configured so as not to flow in one direction through the main 22 and return to the storage unit 21.

Further, the distance between the pair of wall surfaces 31A and 31A can be adjusted by making the side member 31 movable in the container arranging portion 3 or providing a separate mounting plate so as to be detachable. In this case, the filling capacity can be adjusted according to the distance between the pair of wall surfaces 31A and 31A.

Further, although the bottom surface 32A of the container arrangement portion 3 is an inclined surface in the above embodiment, it may be a horizontal surface depending on the shape of the container B.

Further, each wall surface 31A in the container arrangement portion 3 was a flat surface in the above embodiment. However, the present invention is not limited to this, and for example, it may be a curved surface.

Further, the container to be filled with the cell-containing liquid L may be a hard bottle (glass, hard resin bottle, etc.). The container in this case may be closed by the attached lid. In this case, the container arranging portion 3 can be simply a portion on which the container is placed. However, in the manufacturing apparatus 1, a mechanism or control for keeping the filling amount constant is required.

1 ... Manufacturing equipment, 2 ... Liquid supply unit, 3 ... Container arrangement unit, 4 ... Control unit, 21 ... Storage unit, 22 ... Main pipe, 22A ... Main pipe (consolidation pipe), 23 ... Branch pipe, 24 ... Liquid feed pump , 25 ... Liquid recovery pump, 26 ... Main on-off valve (upstream valve), 27 ... Main on-off valve (downstream valve), 28 ... On-off valve, branch on-off valve, 31 ... Side member, 31A ... Wall surface, 32 ... Bottom member, 32A ... bottom surface, 33 ... rear member, 34 ... bag locking part, 211 ... container in storage part, 212 ... lid, 213 ... stirring part, 221 ... upstream side, 222 ... downstream side, B ... container, B1 ... Plug, B11 ... Groove, B2 ... Doorway, B21 ... Doorway tube, L ... Cell-containing liquid

Claims (6)

The main, which is a series of pipelines,
A plurality of branch pipes, which are pipelines branched laterally from the main pipe,
For a combination of a plurality of containers connected to the plurality of branch pipes,
The cell-containing liquid in which the living cells are dispersed is filled in the main pipe in a state where the plurality of branch pipes are closed, and then the plurality of branch pipes are opened, so that the main pipe is used via each branch pipe. A method of supplying a cell-containing liquid to be supplied to each container. The container is a container that expands with the supply of the cell-containing liquid.
The container has an entrance / exit portion for taking in and out the cell-containing liquid, and is closed liquidtightly and airtightly except for the portion of the peripheral portion where the entrance / exit portion is formed.
The method for supplying a cell-containing liquid according to claim 1, wherein the cell-containing liquid is supplied to the container through the inlet / outlet portion, and then the air inside the container is discharged from the inlet / outlet portion. A cell-containing liquid supply device for putting a cell-containing liquid in which living cells are dispersed into a container.
A storage unit that stores cell-containing fluid and
A main pipe, which is a series of pipelines connected to the storage unit,
A plurality of branch pipes, which are pipelines branched laterally from the main pipe,
A liquid feed pump that supplies cell-containing liquid from the reservoir to the main
A plurality of on-off valves provided in the plurality of branch pipes and
With a control unit
The control unit closes the plurality of on-off valves and then operates the liquid feed pump to supply the cell-containing liquid from the storage unit to the main pipe, and the cell-containing liquid is filled in the main pipe. After that, the cell-containing liquid supply device for opening the plurality of on-off valves and supplying the cell-containing liquid from the main pipe to each container via each branch pipe. A liquid recovery pump provided on the side of the main pipe opposite to the side to which the storage portion is connected is provided.
The container is a container that expands with the supply of the cell-containing liquid.
The container has an entrance / exit portion for taking in and out the cell-containing liquid, and is closed in a liquid-tight and airtight manner except for the portion of the peripheral portion where the entrance / exit portion is formed.
The control unit discharges the air inside the container from the inlet / outlet portion of the container by operating the liquid recovery pump after the cell-containing liquid is supplied to the container via the inlet / outlet portion. The cell-containing liquid supply device according to claim 3. A method for producing a cell-containing product in which a container is filled with a cell-containing solution in which living cells are dispersed.
The main, which is a series of pipelines,
A plurality of branch pipes, which are pipelines branched laterally from the main pipe,
For the combination of the plurality of containers connected to the plurality of branch pipes,
A cell containing a cell-containing solution is filled in the main pipe in a state where the plurality of branch pipes are closed, and then supplied to each container from the main pipe via each branch pipe by opening the plurality of branch pipes. Manufacturing method of contained products. The container is a container that expands with the supply of the cell-containing liquid.
The container has an entrance / exit portion for taking in and out the cell-containing liquid, and is closed in a liquid-tight and airtight manner except for the portion of the peripheral portion where the entrance / exit portion is formed.
The method for producing a cell-containing product according to claim 5, wherein the cell-containing liquid is supplied to the container through the doorway, and then the air inside the container is discharged from the doorway.