JP2021014453A - Corolla regulator for plant body - Google Patents

Abstract

To provide a drug making it possible to simply regulate one or more selected from, for example, color tone, fragrance and shape related to a corolla of a plant body according to user's preference without using any special method, such as breeding and gene-recombination.SOLUTION: A corolla regulator for a plant body employs an organic acid and/or a salt thereof as an active ingredient.SELECTED DRAWING: Figure 1

Description

The present invention relates to a plant corolla regulator containing an organic acid and / or a salt thereof as an active ingredient.

Since ancient times, it has been practiced to cultivate plants such as flowers and enjoy watching the color tone and morphology of the plants, or enjoying the aroma of the plants. Among the ornamental plants, roses are especially popular, and in addition to the roses that bloom white and magenta flowers grown in medieval Europe, the roses that came from China added red pigment and pale yellow pigment. Around 1900, a yellow pigment was introduced into the rose flower by crossing with Rosa foetida. In recent years, it is characterized by abundant flower colors compared to other plants, such as the addition of roses that bloom blue flowers by genetic modification. However, little is known about methods for changing the color tone of plant flowers other than breeding and genetic modification.
In addition, many people enjoy the aroma of plants as well as for viewing purposes. Among them, roses have been developed with various characteristic aroma varieties. In recent years, attention has also been paid to the functionality of aroma such as refreshing effect and relaxing effect (for example, Patent Documents 1 and 2). However, as with color tone, little is known about methods for changing the aroma of plant flowers other than breeding and genetic modification.
In addition, the corolla of roses tends to be evaluated as beautiful when the center is high and the petals are tightly rolled and the petals are evenly opened. However, as with the color tone and aroma, other than breeding and genetic recombination, the plant body No method is known to change the shape of the flower.

JP-A-2002-265977 Japanese Unexamined Patent Publication No. 2005-281288

As described above, methods other than breeding and genetic modification are not well known as methods for changing the color tone, aroma, shape, etc. of the corolla, which is an aggregate of petals (petals) in a plant.
Therefore, an object of the present invention is to provide a drug capable of adjusting the color tone, aroma, shape, etc. according to the user's preference without using techniques such as breeding and genetic modification.

As a result of diligent research to solve the above problems, the present inventors have applied an organic acid and / or a salt thereof to the plant, and as a result, with respect to the flower crown of the plant, particularly the color tone, aroma, and shape. Has been found to change, and the present invention has been completed.

The gist of the present invention is as follows.
1. 1. A plant corolla regulator containing an organic acid and / or a salt thereof as an active ingredient.
2. 2. 1. Adjusting one or more selected from the color, aroma and shape of the corolla. The corolla conditioner of the plant described in.
3. 3. A method of adjusting one or more selected from the color, aroma and shape of the corolla of a plant, characterized in that a composition containing an organic acid and / or a salt thereof as an active ingredient is applied to the plant.

According to the present invention, with respect to the corolla of a plant, one or more selected from, in particular, color tone, aroma and shape can be adjusted.
Regarding the color tone of the corolla of a plant, the content of the anthocyanin pigment, which has already been reported to contribute to a relaxing effect and a health effect in food, can be significantly increased by using the adjusting agent of the present invention. Therefore, it is possible to obtain a corolla of a plant with a darker color tone.
Further, regarding the aroma of the corolla of the plant body, the content of the aroma component contained in the corolla of the plant body can be significantly increased, so that the corolla of the plant body having a stronger aroma can be obtained.
Further, since the perfection of the shape of the corolla of the plant can be enhanced, the excellent effect of obtaining the corolla of the plant having an excellent appearance is exhibited.
The present invention is very useful in obtaining a plant having a corolla having an excellent appearance and aroma.

It is a photograph of the corolla of a mini rose using the test samples of Examples 1, 2 and Comparative Example 1 in the color tone adjustment effect confirmation test 1 of the corolla of a plant.

Hereinafter, the present invention will be described in detail.
<Plant corolla modifier>
"Adjustment" in the corolla adjuster of the plant of the present invention means adjusting the corolla of the plant in a direction preferred by the user who uses the adjuster. Specific items to be adjusted include color tone, aroma, size, and three-dimensional shape, and can be used as a corolla color tone adjuster, a corolla aroma adjuster, and a corolla shape adjuster.

<About organic acids and / or their salts>
The active ingredient of the corolla modifier of the plant of the present invention is an organic acid and / or a salt thereof.
Examples of the organic acid in the present invention include a carboxylic acid having a carboxyl group (-CO ₂ H group) and a sulfonic acid having a sulfo group (-SO ₃ H group), and among them, a carboxylic acid is preferable. Examples of the carboxylic acid include saturated carboxylic acids such as formic acid and acetic acid, unsaturated carboxylic acids such as oleic acid, hydroxycarboxylic acids such as malic acid and citric acid, aromatic carboxylic acids such as benzoic acid, oxalic acid and succinic acid. Dicarboxylic acid can be mentioned. Among them, organic acids having 1 to 10 carbon atoms are preferable, and for example, saturated fatty acids such as formic acid, acetic acid, propionic acid, butyric acid, valeric acid, caproic acid, enanthic acid, capric acid, pelargonic acid, and capric acid, oxalic acid, Dicarboxylic acids such as malonic acid, succinic acid, glutaric acid, adipic acid, fumaric acid and maleic acid, hydroxycarboxylic acids such as lactic acid, malic acid, citric acid and tartaric acid, benzoic acid, phthalic acid, isophthalic acid, terephthalic acid and salicylic acid. Such as aromatic carboxylic acids and the like.
Among these organic acids, a saturated carboxylic acid having 1 or more and 5 or less carbon atoms is preferable as the active ingredient of the corolla adjusting agent for the plant body of the present invention.
When acetic acid is used as the active ingredient of the flower crown adjusting agent for plants of the present invention, for example, pure acetic acid, vinegar such as vinegar and synthetic vinegar are included. These are commercially available, and for example, grain vinegar, special concentrated vinegar, high-concentration brewed vinegar, powdered vinegar (mixture of acetic acid and dextrin, etc.) can be used. Fruit vinegars such as wine vinegar and apple vinegar are also available.
Examples of the salt of the organic acid include sodium salt, potassium salt, calcium salt, magnesium salt, ammonium salt, ethanolamine salt, triethanolamine salt and the like, and organic as an active ingredient of the flower crown modifier of the plant of the present invention. When a salt salt is used, sodium salt, triethanolamine salt, ammonium salt and potassium salt are preferable. These salts may be added as a simple substance to the corolla regulator of a plant, or an organic acid and a corresponding neutralizing agent may be added separately to form salts at the time of preparation of the preparation. For example, an organic acid and sodium hydroxide as a neutralizing agent can be added separately and used as a sodium salt. As the neutralizing agent, sodium hydroxide, potassium hydroxide and the like are suitable.
As the corolla regulator of the plant body of the present invention, one containing the above organic acid and / or a salt thereof may be used alone, or two or more thereof may be mixed and used.

The plant corolla regulator of the present invention contains the active ingredient organic acid and / or a salt thereof in an amount of preferably 0.01% by weight or more, more preferably 0.05, based on the total amount of the plant corolla regulator. It can be contained in an amount of% by weight or more, more preferably 0.1% by weight or more. Further, if an organic acid and / or a salt thereof is used in an excessively large amount, some users may be concerned about the odor of the organic acid. Therefore, the content is preferably 10% by weight or less, more preferably 5% by weight or less. More preferably, it is 3% by weight or less.
The corolla adjusting agent for plants of the present invention can be treated as it is on plants, but it is also possible to dilute a preparation containing a predetermined active ingredient with water at the time of use and treat it on plants. In this case, the content of the active ingredient organic acid and / or a salt thereof in the water-diluted preparation is preferably 0.01% by weight or more, more preferably 0.05% by weight or more, still more preferably. It can be prepared and used so as to be 0.1% by weight or more.

The corolla adjusting agent for plants of the present invention can be used as various preparations.
Examples of the preparation include oils, emulsions, wettable powders, flowables (suspensions in water, emulsions in water, etc.), microcapsules, powders, granules, tablets, liquids, sprays, aerosols and the like. Be done. Among them, spray preparations such as spray agents and aerosol agents, spray agents in which a liquid agent is filled in a container with a watering can head, and the like can make the best use of the performance of the corolla conditioner of the plant body of the present invention. Suitable as a formulation type. As a spray agent or an aerosol agent, an aerosol can or a drug bottle equipped with a predetermined spray pattern and a spray device for supplying spray particles can be used.
The corolla adjusting agent for plants of the present invention is not limited to liquid preparations, but can also be used as solid preparations such as powders, granules and fine granules, as long as the effects of the present invention are exhibited.
As one production example of the above preparation, a solution (solution A) is prepared by dissolving it in a solvent using an organic acid and / or a salt thereof and, if necessary, a surfactant, and the solution A is mixed with an appropriate amount of water. , A method of preparing a flower crown adjusting agent for a plant that does not need to be diluted at the time of use can be mentioned by stirring to prepare the preparation.
As the water, tap water, ion-exchanged water, distilled water, filtered water, sterilized water, groundwater and the like can be used.

Examples of the liquid carrier used in the preparation include alcohols (methanol, ethanol, isopropyl alcohol, butanol, hexanol, benzyl alcohol, ethylene glycol, etc.) and ethers (diethyl ether, ethylene glycol dimethyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl). Ether, propylene glycol monomethyl ether, tetrahydrofuran, dioxane, etc.), esters (ethyl acetate, butyl acetate, isopropyl myristate, ethyl lactate, etc.), ketones (acetone, methyl ethyl ketone, methyl isobutyl ketone, cyclohexanone, etc.), aromatic or fat Group hydrocarbons (xylene, toluene, alkylnaphthalene, phenylxysilyl ethane, kerosine, light oil, hexane, cyclohexane, etc.), halogenated hydrocarbons (chlorobenzene, dichloromethane, dichloroethane, trichloroethane, etc.), nitriles (nitrile, isobuty) Lonitrile, etc.), sulfoxides (dimethyl sulfoxide, etc.), heterocyclic solvents (sulfolane, γ-butyrolactone, N-methyl-2-pyrrolidone, N-ethyl-2-pyrrolidone, N-octyl-2-pyrrolidone, 1, 3-Dimethyl-2-imidazolidinone), acid amides (N, N-dimethylformamide, N, N-dimethylacetamide, etc.), alkylidene carbonates (propylene carbonate, etc.), vegetable oils (soybean oil, cottonseed oil, etc.), plants Essential oils (orange oil, hisop oil, pepper oil, lemon oil, etc.), and water can be mentioned.

Examples of the surfactant used at the time of preparation include a nonionic surfactant, an anionic surfactant, a cationic surfactant, and an amphoteric surfactant. Examples of the nonionic surfactant include polyoxyalkylene allylphenyl ether, polyoxyethylene alkyl ether, polyoxyethylene alkyl phenyl ether, polyoxyethylene allyl phenyl ether, polyoxyethylene styryl phenyl ether, and polyoxyethylene alkyl phenyl. Ether formaldehyde condensate, polyoxyethylene-polyoxypropylene block polymer, polyoxyethylene-polyoxypropylene block polymer alkylphenyl ether, sorbitan fatty acid ester (eg, sorbitan monooleate, sorbitan laurate), polyoxyethylene fatty acid ester, Examples thereof include glycerin fatty acid ester, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene hydrogenated castor oil, and polyethylene glycol fatty acid ether. Anionic surfactants include, for example, alkyl sulfate, polyoxyethylene alkyl ether sulfuric acid, polyoxyethylene alkyl phenyl ether sulfuric acid, polyoxyethylene benzyl (or styryl) phenyl ether sulfuric acid or polyoxyethylene-polyoxypropylene block polymer. Sodium, calcium or ammonium salts of sulfuric acid; alkyl sulfonate, dialkyl sulfosuccinate, alkylbenzene sulfonic acid (eg calcium dodecylbenzene sulfonate, etc.), mono- or di-alkylnaphthalene sulfonic acid, formaldehyde condensation of naphthalene sulfonate Mono, lignin sulfonic acid, polyoxyethylene alkylphenyl ether sulfonic acid or polyoxyethylene alkyl ether sulfosuccinate sodium, calcium, ammonium or alkanolamine salts; polyoxyethylene alkyl ether phosphate, polyoxyethylene, mono- Alternatively, salts such as di-alkylphenyl ether phosphate, polyoxyethylene benzyl (or styryl) phenyl ether phosphate, and sodium or calcium salt of polyoxyethylene-polyoxypropylene block polymer phosphate can be mentioned. Examples of the cationic surfactant include a quaternary ammonium salt, an alkylamine salt, an alkylpyridinium salt, an alkyloxide and the like. Examples of amphoteric surfactants include alkyl betaines and amine oxides. The surfactant is also used as a spreading agent.

Examples of the propellant used when the aerosol agent is used include butane gas, chlorofluorocarbon gas, alternative chlorofluorocarbons (HFO, HFC, etc.), liquefied petroleum gas (LPG), dimethyl ether, and carbon dioxide gas.
Examples of solid carriers include clays (kaolin, diatomaceous earth, bentonite, clay, acidic white clay, etc.), synthetic silicon hydroxide-containing silicon hydroxide, talc, zeolite, ceramics, and other inorganic minerals (serisite, quartz, sulfur, activated carbon, calcium carbonate, etc.). Calcium, hydrated silica, etc.), porous materials, etc. can be mentioned.

The plant corolla regulator of the present invention can be added with an antifoaming agent, a preservative, an antioxidant, a thickener and the like, if necessary, at the time of preparation of the preparation.
Examples of the defoaming agent include silicone-based defoaming agents and fluorine-based defoaming agents.
Examples of preservatives include organic nitrogen-sulfur-based compounds, organic bromine-based compounds, isothiazolin-based compounds, benzyl alcohol mono (poly) hemiformal, 1,2-benzoisothiazolin-3-one, and 5-chloro-2-methyl-. Examples thereof include 4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one, 2-bromo-2-nitropropane-1,3-diol, potassium sorbate, sodium dehydroacetate and the like.
Examples of the antioxidant include tetrakis [methylene-3- (3,5-di-t-butyl-4-hydroxyphenyl) propionate] methane, butylated hydroxytoluene (BHT), butylhydroxyanisole (BHA), and propyl gallate. Examples thereof include propyl acid and vitamin E, mixed tocopherol, α-tocopherol, ethoxyquin and ascorbic acid.
Examples of the thickener include polyvinylpyrrolidone, xanthan gum, polyvinyl alcohol, guar gum, carboxyvinyl polymer and the like.

<Corolla adjustment effect>
The plant corolla modifier of the present invention is particularly effective in adjusting one or more selected from the color, aroma and shape of the plant corolla, as will be described in detail in the examples below. ..
By applying the corolla modifier of the present invention to a plant, the content of the anthocyanin pigment contained in the petals can be significantly increased. By utilizing this action, the user can modulate the original color tone of the corolla of the plant body to a deep color tone in the preferred direction.
By applying the corolla modifier of the present invention to a plant, the content of the aroma component contained in the corolla of the plant can be significantly increased. By utilizing this action, the user can modulate the original aroma of the corolla of the plant body to a deep aroma tone in a preferred direction.
Further, by applying the corolla adjusting agent of the present invention to a plant, the degree of perfection of the shape of the corolla of the plant can be enhanced, so that a corolla having an excellent appearance can be obtained.

<About plants>
The plant body in the present invention has a root part and a foliage part, and the root part means a part that is usually in the ground (in some cases, underwater) and supports the above-ground part, and has no root part like a cut branch or a cut flower. The state is not included.
The plant body in the present invention is not limited as long as it is a plant body whose corolla can be adjusted. Specific examples include plants with ornamental corollas such as roses, lilies, carnations, dalia, moth orchids, kiku, turkish ginkgo, cyclamen, statice, symbidium, gerbera, dendrobium, tulips, pelargonium, petunia, and cattleya. ..
The flower crown modifier of the plant of the present invention is not limited to the part of the plant to which it is attached as long as it can be attached to the plant, but it is applied to the foliage and root of the plant from the viewpoint of good absorption efficiency. It is preferable to treat the roots by foliage treatment or irrigation treatment, and it is particularly preferable to apply it to the surface of plant leaves. The time of application may be appropriately selected according to the growing condition of the plant, but it is particularly preferable to apply it before flowering. The frequency of application is preferably once every 1 to 10 days, preferably once every 1 to 7 days, and more preferably once every 1 to 4 days. The means of application is not particularly limited.
The treatment amount of the flower crown adjusting agent for the plant body of the present invention is 0.005 g / week or more and 10 g / week or less in terms of the cumulative treatment amount of the organic acid and / or its salt with respect to the plant body regardless of the application frequency. It is preferable to apply in a range, preferably 0.01 g / week or more and 7 g / week or less, more preferably 0.02 g / week or more and 5 g / week or less. In particular, for plants 10 to 60 cm above the ground, the cumulative treatment amount may be applied in the range of 0.02 g / week or more and 7 g / week or less, preferably 0.05 g / week or more and 5 g / week or less. It is preferable to apply in the range of 0.1 g / week or more, more preferably 3 g / week or less.
In particular, when acetic acid and / or a salt thereof is applied to roses, the cumulative treatment amount per rose is 0.02 g / week or more in the early stage of growth before the emergence of new shoots and the emergence of 5 leaves. It is preferable to apply in the range of 1 g / week or less, preferably 0.05 g / week or more and 0.5 g / week or less. From the initial stage of growth after the emergence of 5 leaves, the cumulative treatment amount per rose strain is in the range of 0.05 g / week or more and 7 g / week or less, preferably 0.1 g / week or more and 5 g / week or less. It is good to apply within the range.

In addition, for example, a fungicide, a fungicide, an insecticidal acaricide, a repellent, a fragrance, an essential oil and the like may be used in combination depending on the purpose. For example, bactericides such as bittertanol, bromconazole, cyproconazole, diphenoconazole, hexaconazole, imazalyl, microbutanyl, simeconazole, tetraconazole, thiabendazole, penthiopyrado, manzeb; benzethonium chloride, benzalkonium chloride, chlorhexidine hydrochloride, glucon Antifungal agents such as chlorhexidine acid, hinokithiol, phenoxyethanol, isopropylmethylphenol; insecticide chrysanthemum extract, natural pyrethrin, prarethrin, imiprothrin, phthalthrin, alesulin, bifentrin, resmethrin, phenothrin, ciphenotrin, permethrin, cypermethrin, ethopheneprox , Bifenthrin, fenvalerate, fenpropatrin, empentrin, silafluofen, transfluthrin, metoflutrin, profluthrin and other pyrethroid compounds, carbamate compounds such as carbalyl, propoxul, mesomil, thiodicalbu, oxaziazole compounds such as methoxadiazone, fipronyl. Phenylpyrazole compounds such as, sulfonamide compounds such as amidoflumeth, neonicotinoid compounds such as dinotefuran and imidacloprid, pyrethroid compounds such as chlorphenapir, fenitrothione, diadinone, marathon, pyridafenthion, prothiophos, hoxim, chlorpyriphos, dichlorovos, etc. Pesticides such as organic phosphorus compounds; Diet, di-n-butyl succinate, hydroxyanisole, rotenone, ethyl-butyl acetylaminopropionate, icaridine (pyrethroid), 3- (N-n-butyl-) One or more repellents such as N-acetyl) aminopropionic acid ethyl ester can be used. As the fragrance and the essential oil, one kind or a combination of two or more kinds appropriately selected from the group consisting of natural fragrances, synthetic fragrances, natural extracts and the like can be used depending on the intended use.

Hereinafter, the present invention will be described in more detail by way of examples, but the present invention is not limited thereto.
In the examples, unless otherwise specified, parts mean parts by weight.

<Test 1 for confirming the effect of adjusting the color tone of the corolla of a plant>
(1) Test sample Example 1
Using 0.125 parts by weight of acetic acid, 0.08 parts by weight of a spreading agent (polyoxyethylene sorbitan monolaurate), and ion-exchanged water, a flower crown modifier for a plant was prepared with a total amount of 100 parts by weight.
Example 2
Using 0.10 parts by weight of acetic acid, 0.08 parts by weight of a spreading agent (polyoxyethylene sorbitan monolaurate) and ion-exchanged water, a flower crown modifier for a plant was prepared with the total amount being 100 parts by weight.
Comparative Example 1
A comparative agent was prepared using 0.08 parts by weight of a spreading agent (polyoxyethylene sorbitan monolaurate) and ion-exchanged water with a total amount of 100 parts by weight.

(2) Test method As a test plant, a mini rose (flower color: pink) with an above-ground part of about 15 to 30 cm grown in a polypot (No. 4: caliber 12 cm x height 10 cm) (test period: August to October) is used. did.
For 2 weeks, the test specimens (Examples 1 and 2, Comparative Example 1) were sprayed on the test plant three times in total on the first day, one week, and two weeks later, so that the entire test plant was sufficiently wet. 80-240 mL was sprayed at a time using a hand spray. It was sprayed before flowering, and the buds and flowers were sprayed so that the liquid agent would not be applied directly.
The color depth of the flowers that bloomed about one month after the first spraying was evaluated by 10 panelists using the flower color of Comparative Example 1 as an evaluation standard (evaluation: 3 points) on the following five stages. .. In this evaluation test, it was judged that the evaluation value of 4 points or more became darker by using the corolla modifier of the plant of the present invention.
It has been confirmed by another test that the spreading agent in the above test sample does not affect the flower color of the plant body.
[Flower color evaluation criteria]
5 points: darker than the flower color of Comparative Example 1 4 points: slightly darker than the flower color of Comparative Example 1 3 points: flower color of Comparative Example 1 2 points: slightly lighter than the flower color of Comparative Example 1 1 point: lighter than the flower color of Comparative Example 1 The evaluation values and average evaluation values of each panelist are shown in Table 1 together with the composition of the test sample.
In addition, photographs of the corolla of mini roses using the test samples of Examples 1, 2 and Comparative Example 1 are shown in FIG.
When each color tone is shown by a Pantone color sample book (SOLID CHIPS / coated: trade name), Example 1 corresponds to 236 U, Example 2 corresponds to 250 U, and Comparative Example 1 corresponds to 671 U.

(3) Results As shown in Table 1, the corolla modifiers of the plants of Examples 1 and 2 containing acetic acid of the present invention as an active ingredient have a flower color based on Comparative Example 1 which does not contain an active ingredient. It became clear that it became darker.
In addition, it was confirmed that there was a difference in the index "completeness" that indicates the appearance of the corolla by evaluating the corolla from the viewpoint of the shape of the petals, the number of petals, and the like. This "completeness" is an index that is evaluated to be higher as the petals open evenly, the number of petals is large, and the central part of the corolla has a raised height (thickness of the corolla).
A confirmation test was conducted on the "completeness" of this corolla.

<Plant corolla shape adjustment effect confirmation test>
(1) Test sample The test sample (Example 1, Comparative Example 1) of the above-mentioned "Test 1 for confirming the effect of adjusting the color tone of the corolla of a plant" was used.
(2) Confirmation test method for corolla shape adjustment effect of plants As a test plant, the above-ground part grown in No. 10 pot (caliber 30 cm x height 30 cm, capacity 11 liters) (test period: September to October) A rose of about 30 to 50 cm (variety: fairy tale kenigin) was used.
For one month from the initial growth of the test plant, use a hand spray on the test sample (Example 1, Comparative Example 1) 2-3 times for 1 week so that the entire test plant is sufficiently wet. 80-300 mL was sprayed at a time. Spraying was started before the buds were formed, and the buds and flowers were sprayed so that the liquid agent would not be applied directly.
The shape of the corolla that bloomed about one month after the first spraying was evaluated according to the following evaluation methods for each of "corolla height", "number of petals", and "corolla circularity".
Corolla height: The corolla height was measured from the side, from the base of the petals to the highest point of the corolla.
Number of petals: The number of all petals forming the corolla was measured.
Circularity of corolla: The circularity of the corolla when viewed from directly above was calculated from the following formula.
[Calculation formula]: Circularity = 4π × (area) ÷ (peripheral length) ²
Both the "area" and "peripheral length" of the corolla are quantified by the image analysis software ImageJ.

(3) Results The average values of the evaluation results of "corolla height", "number of petals", and "corolla circularity" using three rose corollas treated with each test sample are summarized in Table 2 below. Shown. In the "circularity of the corolla" by the above evaluation method, 1.0 is the highest evaluation corolla meaning a perfect circle.

As shown in Table 2, the corolla of the plant body treated with the corolla adjusting agent of the plant body of Example 1 containing acetic acid of the present invention as an active ingredient is compared with the plant body treated in Comparative Example 1 containing no active ingredient. It was clarified that the "corolla height" was increased by about 10% and the "number of petals" was increased by about 20%. Further, the "circularity of the corolla" of the plant treated with the corolla adjusting agent of Example 1 containing acetic acid of the present invention as an active ingredient is 0.96, which is almost a perfect circle. The "circularity of the corolla" of the plant treated in Comparative Example 1 containing no active ingredient was 0.81, and it was also confirmed that the plant had a distorted shape.
From this result, the corolla adjuster of the present invention has a higher shape in all of "corolla height", "number of petals" and "corolla circularity" as compared with the plant body not treated with the corolla adjuster of the present invention. It was clarified that it exerts an adjustment effect and enhances the perfection of the shape of the corolla.

<Test 2 for confirming the effect of adjusting the color tone of the corolla of a plant>
(1) Test sample The test sample (Examples 1 and 2 and Comparative Example 1) of the above-mentioned "Test 1 for confirming the effect of adjusting the color tone of a plant corolla" was used.
(2) Test for confirming the amount of pigment in the corolla of a plant As a test plant, a mini rose with a above-ground part of about 15 to 30 cm grown in a polypot (No. 4: diameter 12 cm x height 10 cm) (test period: August to October) Using (flower color: pink), the test specimens (Examples 1 and 2, Comparative Example 1) were used for 2 weeks, and the whole test plant was sufficiently used 3 times in total on the first day, 1 week, and 2 weeks later. To the extent that it gets wet, 80-240 mL was sprayed at a time using a hand spray. It was sprayed before flowering, and the buds and flowers were sprayed so that the liquid agent would not be applied directly.
Using the corolla that bloomed about 1 month after the first application, the content of anthocyanins contained in the corolla was measured by the following procedure.
0.1 g of petals crushed with liquid nitrogen and 5 mL of a 50% aqueous acetic acid solution were placed in a glass bottle, allowed to stand in a refrigerator (2 ° C.) for 48 hours, and then filtered using a filter (0.45 μm). The anthocyanin content in 0.1 g of petals was calculated under the following analytical conditions.
[Analysis conditions]
Measuring device: High performance liquid chromatography analyzer (manufactured by Shimadzu Corporation)
Column: Inert Sill ODS-2 (4.6 x 150 mm) (manufactured by GL Science)
Oven temperature: 35 ° C
Flow rate: 0.8 mL / min
Mobile phase: (A) 1.5% aqueous phosphoric acid solution, (B) phosphoric acid (1.5% by weight) + acetic acid (20% by weight) + acetonitrile (25% by weight) + water (100% by weight overall) Adjusted to)
Gradient condition: After changing the mobile phase ratio from "(A) 80% by volume + (B) 20% by volume" to "(A) 15% by volume + (B) 85% by volume" over 20 minutes, " (A) 15% by volume + (B) 85% by volume "was maintained for 10 minutes.
Sample injection volume: 20 μL
The test was performed three times, and the average values are summarized in Table 3.

As shown in Table 3, the corolla of the plant body treated with the corolla modifier of Examples 1 and 2 containing acetic acid of the present invention as an active ingredient is the plant body treated in Comparative Example 1 containing no active ingredient. In comparison, it was revealed that the anthocyanin content was dramatically improved. In particular, it was confirmed that the corolla of the plant treated with the corolla adjuster of the plant of Example 1 had an anthocyanin content about twice as much as that of Comparative Example 1.
It was also clarified that the dramatic increase in the amount of anthocyanins contained in the petals was such that the flower color changed deeply.
From this test result, it was clarified that the corolla modifier of the plant body of the present invention has a function as an anthocyanin enhancer in the corolla.

<Aroma adjustment effect confirmation test 1 of plant corolla>
(1) Test sample The test sample (Example 1, Comparative Example 1) of the above-mentioned "Test 1 for confirming the effect of adjusting the color tone of the corolla of a plant" was used.
(2) Confirmation test method of aroma adjusting effect of corolla of plant body As a test plant, the above-ground part grown in No. 10 pot (caliber 30 cm x height 30 cm, capacity 11 liters) (test period: March to April) A rose (variety: fragrant hill) of about 30 to 50 cm was used.
Hand spray the test specimens (Example 1, Comparative Example 1) on the test plant for 2 weeks, 3 times in total on the first day, 1 week, and 2 weeks, until the entire test plant is sufficiently wet. 80-300 mL was sprayed at a time using. Spraying was started before the buds were formed in the early stage of growth, and sprayed so that the buds and flowers were not directly exposed to the liquid agent.
Regarding the aroma of the corolla that bloomed about one month after the first spraying, the aroma of the corolla of Example 1 and Comparative Example 1 was evaluated. Twenty-two panelists (five of whom are odor judges) evaluate the aroma intensity of each corolla, tea-based aroma intensity, fruity-based aroma intensity, and fragrance quality by the following five-step absolute evaluation. Was done. The evaluation standard is set with reference to the evaluation method (9-step pleasant / unpleasant degree display method) used for displaying the degree of pleasantness / discomfort of the odor.
The tea-based scent and the fruity-based scent are two of the rose scents classified into seven types.
[Aroma strength evaluation criteria]
5 points: strong 4 points: slightly strong 3 points: neither strong nor weak 2 points: slightly weak 1 point: weak [fragrance evaluation criteria]
5 points: Good 4 points: Somewhat good 3 points: Not good or bad 2 points: Somewhat bad 1 point: Bad The average value of each panelist's evaluation is summarized in Table 4. It has been confirmed by another test that the spreading agent in the above test sample does not affect the aroma of the corolla of the plant body.

(3) Results As shown in Table 4, the flower canopy of the plant of Example 1 containing the acetic acid of the present invention as the active ingredient was treated with Comparative Example 1 containing no active ingredient. It was evaluated as having a stronger aroma than the plant body. The fragrance was also evaluated as good. As a specific aroma system, the aroma intensity of the tea type and the aroma intensity of the fruity type were stronger in Example 1 than in Comparative Example 1.

<Aroma adjustment effect confirmation test 2 of plant corolla>
(1) Test sample The test sample (Example 1, Comparative Example 1) of the above-mentioned "Test 1 for confirming the effect of adjusting the color tone of the corolla of a plant" was used.
(2) Confirmation test method for the aroma adjusting effect of the corolla of the plant As a test plant, a rose (variety: fragrant hill,) with an above-ground part of about 30 to 50 cm grown in No. 10 pot (test period: March to April) Quinn Elizabeth, Nicole, Double Delight) was used.
[Method of collecting aroma components]
Hand spray the test specimens (Example 1, Comparative Example 1) on the test plant for 2 weeks, 3 times in total on the first day, 1 week, and 2 weeks, until the entire test plant is sufficiently wet. 80-300 mL was sprayed at a time using. Spraying was started before the buds were formed in the early stage of growth, and sprayed so that the buds and flowers were not directly exposed to the liquid agent. The aroma was collected about one month after the start of spraying.
Regarding the aroma of the corolla that bloomed about one month after the first spraying, the flowers were covered with a bag (polyvinyl fluoride, capacity: 2 liters), and a collector (GL Science, MonoTrap RCC18) was placed inside. Then, the aroma component was collected. The collection was carried out for 5 hours (7:30 to 12:30).
[Extraction method of aroma components]
The above-mentioned collecting agent in which dichloromethane and the aroma component were collected was placed in a vial (MT Extract Cup with Vial manufactured by GL Science Co., Ltd.), and the aroma component was measured under the following analytical conditions by sonication for 15 minutes.
[Analysis conditions]
Measuring device: Gas chromatograph mass spectrometer (manufactured by Shimadzu Corporation)
Column: DB-WAX (manufactured by Agilent Technologies)
Column temperature: 40 ° C (5 min) → 4 ° C / min → 250 ° C (5 min)
Carrier gas: helium, 120 kPa
Injection temperature: 250 ° C, Splitless 0.5 min
Detection: MS Scan (m / z: 40-350)
Sample injection volume: 1.0 μL

(3) Results The aroma components of the rose flower treated with the test sample of Example 1 were compared with the aroma components of the rose flower treated with the test sample of Comparative Example 1, and the changed aroma components were compared. The degree of increase is summarized in Table 5 below.

As shown in Table 5, the flower canopy of the plant body treated with the flower crown adjusting agent of the plant body of Example 1 containing acetic acid of the present invention as an active ingredient is compared with the plant body treated with Comparative Example 1 containing no active ingredient. It was confirmed that the aroma components dimethoxytoluene, hydro-β-ionone, and phenethyl alcohol were significantly improved. In particular, it was clarified that the aroma component dimethoxytoluene of Quinn Elizabeth increased dramatically, 8.8 times that of Comparative Example 1, and the aroma component phenethyl alcohol of Fragrant Tohil increased 11.8 times. Although the aroma components differ depending on the variety, it was clarified that the main aroma components in each variety increased.

The corolla modifier of the plant body of the present invention can significantly increase the content of the anthocyanin pigment, which has already been reported to contribute to the relaxing effect and the health effect in food, so that the plant has a darker color tone. You can get the corolla of the body. Further, since the content of the aroma component contained in the corolla of the plant body can be significantly increased, the corolla of the plant body having a strong aroma can be obtained.
The present invention is very useful in obtaining a plant having a corolla having an excellent appearance and aroma.
Further, since the perfection of the shape of the corolla of the plant body can be enhanced, the excellent effect of obtaining the corolla of the plant body having an excellent appearance is exhibited.

Claims (3)

A plant corolla regulator containing an organic acid and / or a salt thereof as an active ingredient. The corolla adjuster for a plant according to claim 1, wherein one or more selected from the color tone, aroma and shape of the corolla are adjusted. A method of adjusting one or more selected from the color, aroma and shape of the corolla of a plant, characterized in that a composition containing an organic acid and / or a salt thereof as an active ingredient is applied to the plant.