JP2021005103A - Slide glass for correcting microscopic image - Google Patents

Abstract

To provide a slide glass for correcting microscopic images, used for reproducibility evaluation of an observation image by a microscope and having high workability of the reproducibility evaluation performed for each objective lens with a different magnification ratio.SOLUTION: A slide glass for correcting microscopic images includes: a first protective base material 1 having at least a light-transmitting part 6; a plurality of correction patterns 3 and 4 observable by transmitted light; and a second protective base material 2 having a spacer 5 and the light-transmitting part 6. Each of the correction patterns 3 and 4 corresponds to an objective lens with a different magnification ratio, has a thin plate-like shape within a range of a visual field, is clamped between the first protective base material 1 and the second protective base material 2 and is disposed not to be overlapped with each other. The spacer 5 is disposed between the first protective base material 1 and the second protective base material 2 so as to retain a gap between the first protective base material 1 and the second protective base material 2, and is disposed to fix the plurality of correction patterns 3 and 4 at an area excluding a plane view area where the at least correction patterns 3 and 4 are provided.SELECTED DRAWING: Figure 6

Description

The present invention relates to a microscope image calibration slide glass used for evaluating the reproducibility of an observed image with a microscope.

In recent years, observation of a sample with a microscope is performed using an imaging device attached to the microscope, and the observation result can be recorded by the microscope with the imaging device, or through an imaging device such as a TV camera or a digital camera. Therefore, the information input from the microscope is output to a display or a printer, which has an advantage that the sample can be evaluated quickly, and is adopted in a wide range of fields such as medical fields.
In such a microscope with an image pickup device, the reproducibility of the image of the image pickup device itself is evaluated, but the reproducibility of the image captured by the image pickup device via the objective lens of the microscope is a method unique to each device. It is evaluated by, and standardization of the evaluation method is desired.
Patent Document 1 relates to a slide glass or the like for providing color information as a comparison standard that can be used for color evaluation and color correction of an image of a measurement sample imaged using a microscope. On the upper surface of the slide glass, a color reference micro color filter is formed so as to correspond to each field of view of objective lenses having different magnifications of the microscope.
However, in Patent Document 1, although it is disclosed that micro color filters for color reference are juxtaposed on the surface of a glass plate, there is a problem of uneven color and density in a minute size for reproducibility evaluation. There is no disclosure of slide glass with a standard color without.

International Publication No. 2004/044639

A slide glass for microscope image calibration used for evaluating the reproducibility of images observed by a microscope. It has a color chart with good color purity and a gray scale with excellent gradation as a calibration pattern, and has different magnifications. There is a demand for a slide glass for microscope image calibration with good workability for reproducibility evaluation performed for each objective lens.

The gist of the first invention for solving the above problems is that it has at least a first protecting group having a translucent portion, a plurality of calibration patterns observable by the transmitted light of a microscope, spacers, and a translucent portion. A slide glass for calibrating a microscope image composed of the protective base material of the above, wherein the first protective base material and the second protective base material have a thin plate shape and a rectangular shape in a plan view, and the calibration Each of the patterns is provided in correspondence with objective lenses having different magnifications of the microscope, and is in the form of a thin plate having a size that can be accommodated in the field of view of each objective lens of the microscope, and is the first protective base material. The spacer is sandwiched between the first protective base material and the second protective base material so as not to overlap with each other, and the spacer is between the first protective base material and the second protective base material, and the first protective base material is provided. A plurality of the calibration patterns arranged so as to hold a gap between the base material and the second protective base material, and at least in a region excluding the plan view region provided with the calibration pattern. It is characterized in that it is arranged so as to fix.
The gist of the second invention for solving the above problem is that in the microscope image calibration slide glass according to the first invention, the calibration pattern is composed of a plurality of independent thin plate-shaped calibration pattern chips. The spacer is characterized in that it is arranged so as to fix a plurality of the calibration pattern chips in a region excluding a plan view region in which the calibration pattern chips are provided. Is.
The gist of the third invention for solving the above problem is that in the slide glass for microscopic image calibration according to any one of the above first and second inventions, the first protective base material and the second protecting group. The material has the translucent portion in a pattern at the same position corresponding to each other in a plan view, and the transmissive portion is sandwiched between the first protective base material and the second protective base material. It is provided so as to correspond to the observation region of the calibration pattern, and is characterized in that a light-shielding portion is provided in a portion of the first protective base material and the second protective base material other than the translucent portion. Is to be.
The gist of the fourth invention for solving the above problem is that in the slide glass for microscopic image calibration according to any one of the first to third inventions, the adhesive layer is the first protective base material and the spacer. It is characterized in that it is provided at least one of the space between the two and the spacer with the second protective base material.
The gist of the fifth invention for solving the above problem is that in the microscope image calibration slide glass according to the fourth invention, the adhesive layer is at least the first protective substrate and the calibration pattern. It is characterized in that it is provided at least one of a part thereof and between the second protective base material and at least a part of the calibration pattern.
The gist of the sixth invention for solving the above problem is the microscope according to any one of claims 1 to 5 in the microscope image calibration slide glass according to any one of the above inventions. The image proofing slide glass is characterized in that the proofing pattern includes a color chart.
The gist of the seventh invention for solving the above problem is that in the microscope image calibration slide glass according to any one of the first to sixth inventions, the calibration pattern includes gray scale. To do.

According to the present invention, by configuring the microscope image calibration slide glass as described above, the calibration pattern is accurately arranged at a specific position in the plan view of the microscope image calibration slide glass. By arranging patterns of sizes corresponding to objective lenses with different magnifications on the same microscope image calibration slide glass, the evaluation of color reproducibility of microscopes and microscopes with an imaging device can be performed with high accuracy and workability. It has a good effect.
In addition, since the calibration pattern chips that make up the calibration pattern are created by cutting them into a predetermined small size and incorporated under the optimum conditions, the calibration pattern chips have uneven colors and shades. It has the effect of ensuring a color chart with good color purity and gray scale with excellent gradation, and has the effect of not causing unevenness, etc., compared to those created by the printing method, inkjet method, photolithography method, etc. Has.

It is a top view and the cross-sectional view which shows the 1st Embodiment of the slide glass for microscopic image calibration. It is sectional drawing which shows the 2nd Embodiment of the slide glass for microscopic image calibration. It is a top view which shows the relationship between a spacer and a pattern chip for calibration in FIG. It is a top view which shows the other arrangement example of the pattern chip for calibration. It is a top view which shows the other arrangement example of the pattern chip for calibration. It is sectional drawing and partial view which shows 3rd Embodiment of the slide glass for microscope image calibration. It is sectional drawing which shows 4th Embodiment of the slide glass for microscopic image calibration. It is sectional drawing which shows 5th Embodiment of the slide glass for microscopic image calibration. It is sectional drawing which shows 6th Embodiment of the slide glass for microscopic image calibration. It is a table which shows the characteristic of the reference color used in a color chart.

Hereinafter, embodiments for carrying out the microscope image calibration slide glass of the present invention will be described with reference to FIGS. 1 to 6.

(First Embodiment)
The first embodiment is composed of at least a first protecting base having a translucent portion, a plurality of calibration patterns observable by the transmitted light of a microscope, a spacer, and a second protective base having a translucent portion. The first protective base material and the second protective base material have a thin plate-like shape and a rectangular shape in a plan view, and each of the calibration patterns has a different magnification of the microscope. It is a thin plate that is provided corresponding to the objective lens and has a size that can be accommodated in the field of view of each objective lens of the microscope, and is sandwiched between the first protective base material and the second protective base material so as not to overlap. The spacer is located between the first protecting base and the second protecting base so as to hold a gap between the first protecting base and the second protecting base. Moreover, it is arranged so as to fix a plurality of calibration patterns in a region excluding a plan view region in which at least a calibration pattern is provided.
FIG. 1 shows an example of a first embodiment of a microscope image calibration slide glass, FIG. 1A is a plan view, and FIG. 1B is a partial cross-sectional view thereof.
As shown in FIG. 1A, the microscope image calibration slide glass 10 has a plurality of calibration patterns 3 arranged at a plurality of locations, here at seven locations. When performing microscope measurement using this microscope image calibration slide glass 10, each time the objective lens is changed, the calibration pattern 3 on the same microscopic image calibration slide glass 10 is changed to one corresponding to each objective lens. That is, the measurement can be performed without replacing the microscope slide glass 10 for microscopic image calibration.
In FIG. 1A, there are seven calibration patterns 3, but the number of calibration patterns 3 corresponds to the number of objective lenses of the microscope. Therefore, the number is not limited to seven.
Further, in FIG. 1A, the calibration pattern 3 is gradually reduced in size from the left side to the right side in the drawing, and the patterns are provided so as to be similar.
As for the size of the calibration pattern 3, in the case of the rectangular calibration pattern 3 as shown in FIG. 1 (a), each magnification of the seven objective lenses of the microscope is set to, for example, 2.5 times, 5 times, and 10 times. It is designed to fit within the field of microscope measurement by each objective lens when it is set to Magnification, 20x, 40x, 60x, and 100x, and it is longer for an objective lens with a magnification of 2.5x. A rectangular calibration pattern 3 having a side of 5.7 mm is used, and a rectangular calibration pattern 3 having a long side of 0.14 mm is used for an objective lens having a magnification of 100 times.
Since the shape of the calibration pattern 3 is not limited, the size of the calibration pattern 3 is appropriately determined so that the shape such as a square, a polygon, or a circle can be accommodated in the field of view of the microscope in addition to the rectangle as described above. Be done.
FIG. 1 (b) shows a cross section of a portion of the microscope image calibration slide glass 10 of FIG. 1 (a) having a calibration pattern 3 at two locations.
As shown in FIG. 1 (b), the calibration pattern 3 is arranged so as not to be sandwiched and overlapped between the first protective base material 1 and the second protective base material 2 which transmit at least visible light. .. These calibration patterns 3 are arranged at the lower part of the second protective base material 2 in the drawing, and allow light from a microscope light source from below to be transmitted for observation.
Further, in FIG. 1B, the spacer 5 is arranged so as to hold a gap between the first protective base material 1 and the second protective base material 2, and at least a calibration pattern 3 is provided. It is provided so as to continuously or discontinuously surround the outer periphery of the calibration pattern 3 so as to fix the plurality of calibration patterns 3 in the region excluding the designated region.

The calibration pattern 3 of the present invention is used for evaluating the reproducibility of a measurement image by a microscope equipped with an imaging device, and includes a color chart, a gray scale, a resolution chart, an inmega chart, a crosshatch chart, and the like. it can.

The first protective base material 1 and the second protective base material 2 of the present invention have at least a translucent portion that transmits visible light, and have a thin plate shape and a rectangular shape in a plan view, and are used for calibration. As the material of pattern 3, it protects from scratches and dust, and glass or plastic can be used. Preferably, glass, which is a material similar to that normally used as a microscope slide glass, is used. Further, in the case of plastic, it is preferable that the material that hinders microscopic measurement does not contain a filler or the like.
The sizes of the first protective base material 1 and the second protective base material 2 are not limited, but the short side is 26 ± 0.1 mm, the long side is 76 ± 0.1 mm, and the thickness. Is preferably 0.2 to 0.4 mm.
This is because the size of the general-purpose microscope slide glass is 26 mm on the short side, 76 mm on the long side, and 1.2 mm in thickness, and therefore, the same size is preferable for the operation of microscope observation.

The spacer 5 of the present invention is arranged between the first protective base material 1 and the second protective base material 2 so as to hold a gap between the first protective base material 1 and the second protective base material 2. The outer circumference of the calibration pattern 3 is continuous or discontinuous so that a plurality of calibration patterns 3 are fixed in a region excluding the plan view region in which the calibration pattern 3 is provided. It is provided so as to surround it.
The shape of the spacer 5 does not necessarily occupy the entire region excluding the plan view region of the calibration pattern 3, and holds a gap between the first protective base material 1 and the second protective base material 2. , The outer circumference of the calibration pattern 3 may be continuously or discontinuously surrounded. Therefore, the spacer 5 may be provided in the same size as the outer periphery of the first and second protective base materials as shown in FIG. 1 (b), and in addition to the spacer 5, the first and second protective base materials may be provided. It may be provided in a size smaller than the outer circumference.
The total thickness of the spacer 5 is preferably 0.02 to 0.4 mm. As the spacer 5, in addition to the spacer 5 made of a single plate, a spacer 5 thinner than the total thickness can be used by stacking a plurality of spacers. When the thickness of the calibration pattern 3 is different, it is possible to stack thin ones and adjust the total thickness according to the number of the thin ones.
As the material of the spacer 5, metal, glass, plastic, or the like can be used, and it is preferable that the spacer 5 is a thin plate, has rigidity, and is easy to process when providing through holes in a pattern. In the case of a metal material, it is preferable to use stainless steel, iron-nickel alloy (42 alloy), copper or the like.
As a method for forming the spacer 5, a method such as punching or etching of a metal plate, through-hole processing by punching of a plastic plate, or molding such as plastic molding or printing can be used.

A method of sealing and adhering a portion of the outer periphery of the microscope image calibration slide glass 10 in the thickness direction after all the members are laminated in order to form the microscope image calibration slide glass 10 in which each member is composed as described above. Alternatively, a method in which fitting portions are provided in advance on the first protective base material 1 and the second protective base material and then fitted together to be united, or a method in which the spacer 5 itself has adhesive strength is used for bonding. Can be used.
Further, when laminating each member, accurate alignment is performed by providing register marks and pin holes on each member of the first protective base material 1, the second protective base material 2, and the spacer 5. be able to.

(Second Embodiment)
The second embodiment is the slide glass for microscopic image calibration of the first embodiment described above.
The calibration pattern is composed of a plurality of independent thin plate-shaped calibration pattern chips, and the spacer is located in a region excluding at least a plan view region in which the calibration pattern chip is provided, and a plurality of calibration pattern chips are provided. It is arranged so as to fix.
FIG. 2 shows an example of the second embodiment of the microscope image calibration slide glass.
FIG. 2A shows a cross section of a portion of the microscope image calibration slide glass 10 having the calibration pattern 3 at two locations.
FIG. 2B shows a first protective base material 1, a calibration pattern 3, and a calibration pattern chip 4 in order to explain the laminated structure of the microscope image calibration slide glass 10 in the cross-sectional view of FIG. 2A. , Spacer 5 and the second protective base material are shown separately.
As shown in FIG. 2A, the calibration pattern 3 is arranged so as not to be sandwiched and overlapped between the first protective base material 1 and the second protective base material 2.
As shown in FIG. 2B, the calibration pattern 3 is composed of a plurality of, here, six calibration pattern chips 4.
In FIG. 2A, the spacer 5 is arranged so as to hold a gap between the first protective base material 1 and the second protective base material 2, and at least a calibration pattern chip 4 is provided. It is provided so as to continuously or discontinuously surround the outer periphery of the calibration pattern chip 4 so as to fix the plurality of calibration pattern chips 4 in the region excluding the area.

FIG. 3 is a plan view showing the positional relationship between the spacer 5 and the calibration pattern chip 4 in FIG. 2 in a plan view.
The head 3 (a) shows only the spacer 5 of the microscope image calibration slide glass 10 shown in FIG. 2, and the portion where the calibration pattern chip 4 of the spacer 5 can be arranged penetrates the front and back surfaces of the spacer 5. It is hollow.
FIG. 3B shows a state in which the calibration pattern chip 4 is fitted in the cavity of the spacer 5.
The spacer 5 is provided so as to continuously or discontinuously surround the outer periphery of the calibration pattern chip 4. With this spacer 5, the calibration pattern chip 4 can be accurately arranged at a predetermined position in the microscope image calibration slide glass 10.

Here, a color chart as an example of the calibration pattern 3 will be described.
The color chart is composed of, for example, red (R), green (G), blue (B), yellow (Y), cyan (C), and magenta (M) as colors for color evaluation. ..
Therefore, the color chart which is the calibration pattern 3 is an arrangement of each color for color evaluation as the calibration pattern chip 4, and the calibration pattern chip 4 has a size larger than that of the calibration pattern chip 4 for each color for color evaluation. It is formed by cutting the one created in.
Here, as a color chart in which each color for color evaluation is created with a size larger than the calibration pattern chip 4, for example, a color chart for an imaging device as shown in FIG. 10 is used as a reference color (manufactured by Dainippon Printing Co., Ltd .: " A standard color bar chart ") can be used.
The above "standard color bar chart" is composed of pre-designed red (R), green (G), blue (B), yellow (Y), cyan (C), and magenta (M). The effective size is 175 mm x 245 mm and is composed of 6 color bars.
As a method for producing the above "standard color bar chart", a silver salt emulsion prepared by adding a solution of potassium bromide and silver nitrate to gelatin is applied on a glass plate and desilvered from a dried silver salt photographic plate. A method of forming the substrate by dyeing the substrate with a dye corresponding to each color can be used.

When the above "standard color bar chart" is cut to the size of the calibration pattern chip 4 and used, the calibration pattern chip 4 has the effect of ensuring stable characteristics without color unevenness or shading unevenness. Even if the calibration pattern chip 4 has a minute size corresponding to a high-magnification objective lens, its purity is maintained as a reference color for reproducibility evaluation. Withstand For example, compared to the case where an attempt is made to form a standard color proofing pattern chip 4 similar to the "standard color bar chart" on glass by a printing method, an inkjet method, a photolithography method, or the like, printing ink or It has the effect that there is no problem of deterioration of color purity due to adjustment of the coating liquid and reduction of color purity due to uneven film thickness of the formed film.

The reference color is not limited to the above six colors of red (R), green (G), blue (B), yellow (Y), cyan (C), and magenta (M).
A method using a color corresponding to the observation target as a reference color can be adopted. For example, when observing a biological tissue by staining it, the biological tissue is stained with a standard staining method such as hematoxylin / eosin staining or other staining methods, such as reddish color, greenish color, and bluish color. A color selected from a color, a cyan color, a magenta color, a yellow color, another system color, or the like can be used as a reference color.

The arrangement of the calibration pattern chips 4 in each calibration pattern 3 may be arranged in a grid pattern or a circular shape in addition to the case where the calibration pattern chips 4 are arranged in a row as shown in FIG. 3 (b). ..
FIG. 4 shows an example in which the calibration pattern chips 4 of the color chart are arranged in a 2 × 3 grid pattern.
As shown in FIG. 4, in the calibration pattern 3, for example, the calibration pattern chips 4 are arranged in the order of red (R), green (G), and blue (B) from the upper right to the left in the figure. Cyan (C), magenta (M), and yellow (Y) are arranged in this order from the lower left to the right in the figure. It also has a colorless and translucent blank area between the upper and lower tiers.

Further, as an example of the calibration pattern 3 of the present invention, the case of gray scale will be described.
The gray scale is a scale that expresses the gradation by gradually changing the reflection density in the gray range in the middle from white to black in the reflection type, and the gradation by gradually changing the transmittance in the transmission type. It is a scale that expresses. Here, a transparent gray scale is used.
The gray scale, which is the calibration pattern 3, is configured by arranging calibration pattern chips 4 for each gradation having different transmittances. As an example of the arrangement, as shown in FIG. 3B, there is a grid-like arrangement in addition to the arrangement in which the transmittance gradually decreases from the left side to the right side in the drawing.
FIG. 5 shows an example in which the grayscale calibration pattern chip 4 is configured in two rows.
As shown in FIG. 5, in the calibration pattern 3, the calibration pattern chip 4 has a transmittance of 1.86% to 77.14% in 6 tones from the lower right to the left in the figure, and the upper right. From to the left, the transmittance is arranged in 6 tones from 51.43% to 83.57%.
Therefore, the gray scale, which is the calibration pattern 3, is an arrangement of chips of each gradation from the one having a small transmittance to the one having a large transmittance as the calibration pattern chip 4, and the calibration pattern chip 4 is more than the calibration pattern chip 4. It is formed by cutting a large size product.
As the gray scale, for example, a chromium nickel alloy can be formed by a sputtering method by the same forming method as an ND filter (neutral density filter).
When the above ND filter is cut to the size of the calibration pattern chip 4 and used, the calibration pattern chip 4 has an effect of ensuring stable characteristics without unevenness in shading. Even if the calibration pattern chip 4 has a small size corresponding to a high-magnification objective lens, its purity is maintained as a reference color for reproducibility evaluation. For example, when the calibration pattern chip 4 obtained by obtaining the gray scale as described above is attempted to be formed directly on the glass in the size of the calibration pattern chip 4 by a printing method, an inkjet method, a photolithography method, or the like. Compared with the above, there is an effect that there is no problem of deterioration of gradation due to blending of printing ink and coating liquid and uneven film thickness of the formed film.

Since the microscope image calibration slide glass 10 is configured as described above, the calibration pattern 3 accurately positions the microscope image calibration slide glass 10 at a specific position in the plan view, and the objective lenses having different magnifications. By arranging a plurality of calibration patterns having a size that fits in the field of view, there is an effect that the color reproducibility of the microscope and the microscope with an imaging device can be evaluated with high accuracy and good workability.
When the calibration pattern 3 is a color chart, the calibration putter chip 4 is used by cutting a size larger than the size of the calibration pattern chip 4 for each color. The pattern chip 4 has the effect of ensuring stable characteristics without color unevenness or shading unevenness. As a comparison, there is no problem of color and density unevenness in a minute size that tends to occur when a calibration pattern chip 4 is produced on a microscope image calibration slide glass 10 by a printing method, an inkjet method, a photolithography method, or the like. Has an effect.

(Third Embodiment)
In the third embodiment, in the slide glass for microscopic image calibration according to the first to second embodiments, the first protective base material and the second protective base material are patterned at the same positions corresponding to each other in a plan view. It has the translucent portion, and the translucent portion is provided so as to correspond to the observation region of the calibration pattern sandwiched between the first protective base material and the second protective base material. A light-shielding portion is provided in a portion of the protective base material and the second protective base material other than the translucent portion.
FIG. 6 is a cross-sectional view and a partial view showing an example of the third embodiment of the microscope image calibration slide glass.
FIG. 6A shows a cross section of a portion of the microscope image calibration slide glass 10 having the calibration pattern 3 at two locations.
FIG. 6B is a first protective base material 1 having a light transmitting portion 6 and a light shielding portion 7 in order to explain the laminated structure of the microscope image calibration slide glass 10 in the cross-sectional view of FIG. 6A. The second protective base material having the calibration pattern 3, the calibration pattern chip 4, the spacer 5, the light transmitting portion 6 and the light shielding portion 7 is shown separately.
FIG. 6C is a partial view showing the structural relationship between the second protective base material having the light transmitting portion 6 and the light shielding portion 7 and the calibration putter chip 4.

As shown in FIG. 6A, the calibration pattern 3 is arranged so as not to be sandwiched and overlapped between the first protective base material 1 and the second protective base material 2.
As shown in FIG. 6B, the calibration pattern 3 is composed of a plurality of, here, six calibration pattern chips 4.
In FIG. 6A, the spacer 5 is arranged so as to hold a gap between the first protective base material 1 and the second protective base material 2, and at least a calibration pattern chip 4 is provided. It is provided so as to continuously or discontinuously surround the outer periphery of the calibration pattern chip 4 so as to fix the plurality of calibration pattern chips 4 in the region excluding the area.
As shown in FIG. 6A, in the microscope image calibration slide glass 10, both the first protective base material 1 and the second protective base material 2 are transparent in a pattern at the same positions corresponding to each other in a plan view. It has a light unit 6 and a light-shielding unit 7 (hereinafter referred to as a “light-shielding pattern”).
In FIG. 6A, the translucent portion 6 corresponds to the observation region of the calibration pattern 3 or the calibration pattern chip 4 sandwiched between the first protective base material 1 and the second protective base material 2. When the microscope image calibration slide glass 10 is installed in the microscope, the calibration pattern 3 or the calibration pattern chip 4 is provided so that the light from the light source provided in the microscope can be transmitted. There is.
The light-shielding portion 7 is provided in a portion of the first protective base material 1 and the second protective base material 2 excluding the translucent portion 6.
FIG. 6C is a view of the laminated state of the second protective base material 2 having a light-shielding pattern and the calibration pattern chip 4 in FIG. 6A as viewed from the calibration pattern chip 4 side.
As shown in FIG. 6 (c), an example of the relationship between the light-shielding pattern formed on the second protective base material 2 shown in FIG. 6 (a) and the calibration pattern chip 4 is shown.
As shown in FIG. 6C, in the relationship between the calibration pattern chip 4 and the translucent portion 6 of the corresponding light-shielding pattern, the calibration pattern chip 4 is larger in size than the corresponding translucent portion 6 and is a calibration pattern chip. It is preferable that 4 covers all the corresponding translucent portions 6 and further exceeds the outer peripheral portion.
The above embodiment has described the case where the first protective base material and the second protective base material have a light-shielding pattern in the second embodiment, but the same can be performed in the first embodiment as well. In that case, the calibration pattern 3 is handled as one, and the calibration pattern 3 is larger in size than the corresponding light-transmitting portion 6 in the relationship between the calibration pattern 3 and the light-transmitting portion 6 of the corresponding light-shielding pattern, and is calibrated. It is preferable that the pattern 3 covers all the corresponding translucent portions 6 and further extends beyond the outer peripheral portion.

The first protective base material 1 and the second protective base material 2 having the light-shielding pattern of the present invention can be formed by a silver salt photography method, a printing method, a photolithography method, or the like.
According to the silver salt photographic method, the silver salt emulsion layer surface on the transparent substrate is exposed and developed by a laser drawing method or a method via a photomask to have a patterned translucent portion 6 and a light-shielding portion 7. The first protective base material 1 and the first protective base material 2 can be prepared.
Further, according to the printing method, a first protective base material having a patterned light-transmitting portion 6 and a light-shielding portion 7 by printing a black pattern on a transparent base material by gravure printing with black ink. The first and the second protective base material 2 can be prepared.
Further, according to the inkjet method, the first protective base material 1 and the first protective base material 1 having the patterned light-transmitting portion 6 and the light-shielding portion 7 by ejecting ink with black ink onto the transparent base material. The protective base material 2 can be prepared.

Since the microscope image calibration slide glass 10 is configured as described above, the calibration pattern 3 or the calibration pattern chip 4 is accurately arranged at a specific position in the plan view of the microscope image calibration slide glass 10. The first protective base material 1 and the second protective base material 2 have a patterned light-transmitting portion 6 and a light-shielding portion 7 at the same positions corresponding to each other in a plan view, and the light-transmitting portion 6 Is provided for transmitting the calibration pattern 3 or the calibration pattern chip 4 when the microscope image calibration slide glass 10 is installed in the microscope, so that the calibration pattern 3 or the calibration pattern chip 4 can transmit light. Since it becomes difficult for the light of the microscope other than the part of the pattern chip 4 to enter the eye or the imaging device indirectly into the eye or the imaging device, the color reproducibility of the microscope and the microscope with the imaging device can be evaluated with high accuracy and work efficiency. Has an effect.

(Fourth Embodiment)
In the fourth embodiment, in the slide glass for microscopic image calibration of the first to third embodiments, the adhesive layer 8 is between the first protective base material 1 and the spacer 5, and the second protective base material. It is provided on at least one of the spacer 5 and the spacer 5.
FIG. 7 is a cross-sectional view showing an example of the fourth embodiment of the microscope image calibration slide glass.
FIG. 7A is a cross-sectional view, and FIG. 7B shows a first protective base material 1, a calibration pattern 3, a spacer 5, an adhesive layer 8 and a second layer in order to make the laminated structure easy to understand. The protective base material 2 is shown separately.
As shown in FIGS. 7A and 7B, in the microscope image calibration slide glass 10, the adhesive layer 8 is between the first protective base material 1 and the spacer 5, and the second protective base material 2. It is provided both between the spacer 5 and the spacer 5.

The adhesive layer 8 is located between the first protective base material 1 and the spacer 5, and either or both of the second protective base material 2 and the spacer 5, and adheres between the members. Is.
The adhesive layer 8 is formed so that the slide glass 10 for microscopic image calibration in the adhered state has no distortion in appearance and is not defocused as a whole when placed on a microscope table and observed. ..
As the material of the adhesive layer 8, a liquid or tape-shaped adhesive or adhesive can be used. Of these, tape-shaped ones are preferable, and there is an advantage that the calibration pattern 3 is not contaminated by permeation of the adhesive or the like.

Since the microscope image calibration slide glass 10 is configured as described above, the calibration pattern 3 is accurately arranged at a specific position in the plan view of the microscope image calibration slide glass 10, and is adhered. The portion having the layer 8 is located between the first protective base material 1 and the spacer 5, and either or both of the second protective base material 2 and the spacer 5, so that between the members. It can be fixed stably, and has the effect of being easily fixed without high-precision processing such as fitting the calibration pattern 3 into the spacer 5.

(Fifth Embodiment)
In a fifth embodiment, in the microscope image calibration slide glass of the fourth embodiment, the adhesive layer is between the first protective base material and a part of the calibration pattern and between the second protective base material and the calibration. It is also provided on at least one of the patterns.
FIG. 8 is a cross-sectional view showing an example of the fifth embodiment of the microscope image calibration slide glass.
8 (a) is a cross-sectional view, and FIG. 8 (b) shows a first protective base material 1, a calibration pattern 3, a spacer 5, an adhesive layer 8 and a second layer in order to make the laminated structure easy to understand. The protective base material 2 is shown separately.
As shown in FIGS. 8A and 8B, in the microscope image calibration slide glass 10, the portion having the adhesive layer 8 is between the first protective base material 1 and a part of the calibration pattern 3 and It is both between the second protecting base 2 and a portion of the calibration pattern 3.
The adhesive layer 8 is formed so as to overlap a part of the calibration pattern 3, and can fix the members even if there is a gap in plan view between the calibration pattern 3 and the spacer 5. Is.
A portion of the adhesive layer 8 that overlaps a part of the calibration pattern 3 is provided in a region that is not affected by microscopic observation, and is formed on the light-shielding portion 7 of the first protective base material 1 and the second protective base material 2. It is preferable that the area is covered with the corresponding area.
It is preferable that the adhesive layer 8 is provided with an adjusted thickness so that the adhered slide glass 10 for microscopic image calibration is not distorted in appearance.

(Sixth Embodiment)
In the sixth embodiment, in the microscope image calibration slide glass of the fourth to fifth embodiments, the adhesive layer is calibrated between the first protective base material and the calibration pattern and with the second protective base material. It is also provided on at least one of the patterns.
FIG. 9 is a cross-sectional view showing an example of the sixth embodiment of the microscope image calibration slide glass.
9 (a) is a cross-sectional view, and FIG. 9 (b) shows a first protective base material 1, a calibration pattern 3, a spacer 5, an adhesive layer 8 and a second layer in order to make the laminated structure easy to understand. The protective base material 2 is shown separately.
As shown in FIGS. 9A and 9B, in the microscope image calibration slide glass 10, the adhesive layer 8 is between the first protective base material 1 and the calibration pattern 3, and the first protective base material. It is provided between the spacer 5 and the second protective base material 2 and the calibration pattern 3, and between the second protective base material and the spacer 5.
Since the adhesive layer 8 is formed on the calibration pattern 3, it is measured through an adhesive during microscopic observation. Therefore, it is preferable that the adhesive 8 is made of a material that does not affect the measurement, has excellent light transmittance, and does not contain a filler or the like.
According to the structure of the adhesive layer 8 as described above, as a method of manufacturing the slide glass 10 for microscopic image calibration, the adhesive layer 8 is formed on the entire surface of the first protective base material 1, and a spacer is provided on the adhesive layer side. A method can be used in which 5 are laminated, the calibration pattern 3 is placed at a predetermined position of the spacer 5, and then the second protective base material on which the adhesive layer is formed is laminated.

Since the microscope image calibration slide glass 10 is configured as described above, the calibration pattern 3 is accurately arranged at a specific position in the plan view of the microscope image calibration slide glass 10, and is adhered. The portion having the layer 8 is located between the first protective base material 1 and the spacer 5, and either or both of the second protective base material 2 and the spacer 5, and the first protective base material is provided. By being between 1 and a part of the calibration pattern 3 and / or both of the second protective substrate 2 and a part of the calibration pattern 3, the calibration pattern 3 to the spacer 5 It has the effect of being easily fixed without high-precision processing such as fitting.

1 First protective base material 2 Second protective base material 3 Calibration pattern 4 Calibration pattern chip 5 Spacer 6 Translucent part 7 Light-shielding part 8 Adhesive layer 10 Microscope image calibration slide glass

Claims (1)

It is composed of at least a first protective base material having a translucent portion, a plurality of calibration patterns observable by the transmitted light of a microscope, a spacer, and a second protective base material having a translucent portion. A slide glass for calibrating a microscope image
The first protective base material and the second protective base material are in the form of a thin plate.
Each of the calibration patterns is provided corresponding to objective lenses having different magnifications of the microscope, and is a thin plate having a size that can be accommodated in the field of view of each objective lens of the microscope, and the first protection. It is sandwiched between the base material and the second protective base material and arranged so as not to overlap.
The spacer is arranged between the first protective base material and the second protective base material so as to hold a gap between the first protective base material and the second protective base material. And are arranged so as to fix the plurality of the calibration patterns.
The calibration pattern is composed of a plurality of independent thin plate-shaped calibration pattern chips.
In the calibration pattern, the calibration pattern chip is arranged with red (R), green (G), blue (B), cyan (C), magenta (M) and yellow (Y), and is colorless and translucent. A glass slide for microscope image calibration, which has a blank area of.