JP2020516912A - 粒子懸濁液の顕微鏡撮像及びラマン分光材料分析のための液体セル - Google Patents
粒子懸濁液の顕微鏡撮像及びラマン分光材料分析のための液体セル Download PDFInfo
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Abstract
Description
底部と、底部の反対側の測定窓と、シールとを含む測定室を有しており、
底部は少なくともシールの支持面の領域で平坦にされており、
底部は特に平滑な、特に部分的に平滑な反射表面を有しており、この反射表面は、測定窓を通って入射する光、特にラマン励起光が反射表面で方向付けられて反射されるようになっているため、ラマン測定時のバックグラウンド信号が減少し、懸濁液に含まれている粒子のラマン信号が増加する。
液体セルを閉じて固定するための保持装置と、
本発明による液体セルとを有するシステムであって、
保持装置は液体セルを滑り込ませることができるスライドガイドを有しており、このスライドガイドは、液体セルが保持装置に受容されると測定窓がシールを押圧するように構成された押圧板を有するシステムによって解決される。
図6には、本発明による液体セルの組立が一連の画像として示されている。組立の手順はA)から始まり、F)まで実行されて組み立てられた液体セルになる。
A)モジュール式液体セルのすべての個別部材はまだ組み合わされておらず、ばらばらに存在している。この例では個別部材は底部3、測定窓5(ここでは破線の境界線で示されている)、挿入部材20としてのインサートディスク、スペーサ部材21としてのワッシャ、シール6、及び押圧部材9である。この例では底部3に流入開口部と流出開口部がない。しかしながら代替としてこれらの開口部71、72を備えた底部3も考えられる。
B)挿入ディスク20が底部3の凹部8に挿入される。
C)次にシール6が底部3の凹部8内のインサートディスク上に置かれる。
D)続いてスペーサ部材21が底部3の上に置かれる。
E)次に測定窓5がシール6上に置かれる。
F)そして押圧部材9が測定窓5と距離要素21の上に置かれる。
液体セル1への試料液の充填は、開いた液体セル1(測定窓5が開いているか、測定窓5がない)、又は閉じた液体セル1(測定窓5が閉じている)で行うことができる。閉じた液体セル1では、試料液は流入開口部71と流出開口部72を通して導入又は排出可能である。
適切な顕微鏡は、画像取得と粒子認識のために10倍(通常NA0.25)又は20倍(通常NA0.45)の対物レンズを備えている。対応する被写界深度は、約20μm又は約5μmである。この場合、画像取得は好ましくは暗視野照明で実行されるが、これは底部3の反射表面4が平滑であるために可能である。これにより粒子と背景のコントラストが高くなり、自動のコンピュータ支援粒子認識が改善される。
Claims (10)
- 反射光顕微鏡における粒子懸濁液の顕微鏡画像取得及びラマン分光材料分析のための液体セル(1)であって、前記液体セル(1)は、底部(3)と、前記底部(3)の反対側の測定窓(5)と、シール(6)とを少なくとも含む、測定室(2)を有しており、前記底部(3)は少なくとも前記シール(6)の支持部の領域で平坦にされている、液体セル(1)において、
前記底部(3)は反射表面(4)を有しており、前記反射表面(4)は、前記測定窓(5)を通って入射するラマン励起光が前記反射表面(4)で方向付けられて反射されるようになっていて、粒子のラマン信号が特に2回の励起機会に基づいて増加することを特徴とする液体セル。 - 前記底部(3)の前記反射表面(4)は金属又は金属合金を有し、特に前記底部(3)は金属又は金属合金からなることを特徴とする、請求項1に記載の液体セル。
- 前記反射表面(4)は前記底部(3)上に配置された反射皮膜(40)であり、前記反射皮膜(40)は特に半透明であることを特徴とする、請求項1又は2に記載の液体セル。
- 前記底部(3)は、前記測定室(2)内で充填し、排出し、真空化し、及び/又は貫流を生成するための流入開口部(71)と流出開口部(72)を有しており、前記流入開口部及び/又は流出開口部(71、72)は特に前記測定室(2)の一部である底部(3)の領域に配置されていることを特徴とする、請求項1〜3のいずれか一項に記載の液体セル。
- 前記反射表面(4)は、300nm〜800nm、特に210nm〜1200nmの波長範囲で連続的に反射することを特徴とする、請求項1〜4のいずれか一項に記載の液体セル。
- 前記液体セル(1)は、スライドガイド内に前記液体セル(1)が導入され得るように構成された押圧部材(9)を有しており、前記押圧部材(9)はスライドガイドによって押し下げられて、前記押圧部材(9)は前記測定窓(5)と前記シール(6)と底部(3)と、特に下部(30)とを押圧し、その結果として、測定室(2)が密閉され、前記押圧部材(9)はその第1の領域が前記測定窓(5)の上又は上方に配置されていて、その第2の領域が底部(3)の上又は上方に配置されていることを特徴とする、請求項1〜5のいずれか一項に記載の液体セル。
- 前記液体セルはスペーサ部材(21)を有しており、前記スペーサ部材(21)は前記液体セル(1)の底部(3)の上に、特に測定室(2)の外部で前記液体セル(1)の底部(3)の上に配置されており、前記押圧部材(9)は第2の領域でスペーサ部材(21)の上に載っていて、前記測定窓(5)は前記押圧部材(9)により所定の距離まで底部(3)に押圧されることができ、その際に前記スペーサ部材(21)の高さが前記測定窓(5)の底部(3)からの距離を指定することを特徴とする、請求項6に記載の液体セル。
- 液体セル(1)を閉じて固定するための保持装置(200)と、
請求項1〜7のいずれか一項に記載の液体セル(1)と、を有するシステムであって、
前記保持装置(200)は前記液体セル(1)を滑り込ませることができるスライドガイド(201)を有しており、前記スライドガイド(201)は、前記液体セル(1)が保持装置(200)に受容されると前記測定窓(5)が前記シール(6)を押圧するように構成された押圧板(202)を有するシステム。 - 粒子懸濁液の顕微鏡画像取得及びラマン分光材料測定のための手段を有する顕微鏡であって、前記顕微鏡は反射光顕微鏡であり、前記顕微鏡は請求項1から7のいずれか一項に記載の液体セル(1)、又は特に請求項8に記載のシステムを有する顕微鏡。
- 前記顕微鏡は暗視野照明用に構成されており、前記液体セル(1)の底部(3)の反射表面(4)は入射する暗視野照明の反射体として用いられることを特徴とする、請求項9に記載の顕微鏡。
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