JP2020137476A - Beverage/food product including milk containing lactic acid bacteria - Google Patents
Beverage/food product including milk containing lactic acid bacteria Download PDFInfo
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- 235000013305 food Nutrition 0.000 title claims abstract description 97
- 241000894006 Bacteria Species 0.000 title claims abstract description 59
- 235000013361 beverage Nutrition 0.000 title claims abstract description 23
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 title claims description 44
- 239000004310 lactic acid Substances 0.000 title claims description 22
- 235000014655 lactic acid Nutrition 0.000 title claims description 22
- 241000186605 Lactobacillus paracasei Species 0.000 claims abstract description 67
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- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
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- 229910021380 Manganese Chloride Inorganic materials 0.000 description 1
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Landscapes
- Dairy Products (AREA)
Abstract
Description
本発明は、乳酸菌を含有する乳入り飲食品に関する。より具体的には、一定量以上の無脂乳固形分を含む乳酸菌を含有する乳入り飲食品の塩味低減の技術に関する。 The present invention relates to milk-containing foods and drinks containing lactic acid bacteria. More specifically, the present invention relates to a technique for reducing the salty taste of milk-containing foods and drinks containing lactic acid bacteria containing a certain amount or more of non-fat milk solids.
従来、乳酸菌の種々の生理作用が研究され、現在では、有用な乳酸菌を含む飲食品が開発されている。
また、近年、乳原料から製造される飲食品(乳入り飲食品)の分野では、タンパク質を含む無脂乳固形分を高めた製品が、そのコク等の高級感や栄養価の観点から人気がある。
Conventionally, various physiological actions of lactic acid bacteria have been studied, and nowadays, foods and drinks containing useful lactic acid bacteria have been developed.
In recent years, in the field of foods and drinks produced from dairy raw materials (foods and drinks containing milk), products with a high solid content of non-fat milk containing protein have become popular from the viewpoint of their richness and nutritional value. is there.
しかしながら、無脂乳固形分が高い乳入り飲食品の製造においては、無脂乳固形分を有する乳原料を多量に添加することが必要となる。その結果、製造される飲食品中に、乳原料由来のタンパク質等の他にナトリウム等のミネラルも多く含有されるため、飲食品の塩味が問題となる。
これに対して、従来、無脂乳固形分が高い乳入り飲食品を製造するにあたっては、乳原料及び/又は製造した乳入り飲食品を膜処理することで、ナトリウムを除去し、塩味を低減することが行われていた。
例えば、特許文献1には、発酵乳ミックスを発酵させて得られた発酵物を膜処理することで濃厚な食感を有し、塩味が抑えられた、後味の良い滑らかな食感の発酵乳が得られることが記載されている。
However, in the production of milk-containing foods and drinks having a high non-fat milk solid content, it is necessary to add a large amount of a dairy raw material having a non-fat milk solid content. As a result, the produced foods and drinks contain a large amount of minerals such as sodium in addition to proteins derived from dairy raw materials, so that the salty taste of the foods and drinks becomes a problem.
On the other hand, in the conventional production of milk-containing foods and drinks having a high non-fat milk solid content, sodium is removed and saltiness is reduced by film-treating the milk raw material and / or the produced milk-containing foods and drinks. Was being done.
For example, Patent Document 1 describes fermented milk having a rich texture by membrane-treating a fermented product obtained by fermenting a fermented milk mix, suppressing saltiness, and having a smooth texture with a good aftertaste. Is stated to be obtained.
また、飲料の塩味のマスキングに、アルギン酸と特定のオリゴ糖を用いる例(特許文献2)、果糖、リンゴ酸を用いる例(特許文献3)が知られている。 Further, there are known examples of using alginic acid and a specific oligosaccharide (Patent Document 2) and fructose and malic acid (Patent Document 3) for masking the salty taste of beverages.
前述した従来技術に示される、乳原料及び/又は乳入り飲食品を膜処理する方法では、低分子のミネラルや香気成分も除去される結果、栄養素の不足や風味の低下が問題となっていた。そのため、無脂乳固形分の高い乳入り飲食品の製造において膜処理を行わずに塩味を低減することが求められていた。
本発明は、塩味を有する乳入り飲食品の塩味を低減する手段を提供することを課題とする。そして、本発明は、無脂乳固形分を多く含み、塩味が低減された乳入り飲食品を提供することを課題とする。
In the method of membrane-treating dairy raw materials and / or milk-containing foods and drinks shown in the above-mentioned prior art, low-molecular-weight minerals and aroma components are also removed, resulting in problems such as lack of nutrients and deterioration of flavor. .. Therefore, in the production of milk-containing foods and drinks having a high non-fat milk solid content, it has been required to reduce the salty taste without performing a membrane treatment.
An object of the present invention is to provide a means for reducing the salty taste of a milky food or drink having a salty taste. An object of the present invention is to provide a milk-containing food or drink containing a large amount of non-fat milk solids and having a reduced salty taste.
本発明者らは、ラクトバチルス・パラカゼイ(Lactobacillus paracasei)が、無脂乳固形分を多く含む乳入り飲食品の塩味を低減する効果を奏することを見出し、本発明を完成させた。 The present inventors have found that Lactobacillus paracasei has the effect of reducing the salty taste of milk-containing foods and drinks containing a large amount of non-fat milk solids, and have completed the present invention.
すなわち、前記課題を解決する本発明は、無脂乳固形分が9質量%以上であり、ラクトバチルス・パラカゼイ(Lactobacillus paracasei)を含む、乳入り飲食品である。
これにより、無脂乳固形分を多く含む乳入り飲食品の塩味を低減することができる。
That is, the present invention that solves the above-mentioned problems is a food or drink containing milk having a non-fat milk solid content of 9% by mass or more and containing Lactobacillus paracasei.
This makes it possible to reduce the salty taste of milk-containing foods and drinks containing a large amount of non-fat milk solids.
本発明の好ましい形態では、前記ラクトバチルス・パラカゼイ(Lactobacillus paracasei)は、ラクトバチルス・パラカゼイ(Lactobacillus paracasei)MCC1849株(NITE BP−01633)である。 In a preferred embodiment of the present invention, the Lactobacillus paracasei is the Lactobacillus paracasei MCC1849 strain (NITE BP-01633).
本発明の好ましい形態では、前記ラクトバチルス・パラカゼイ(Lactobacillus paracasei)が死菌である。
これにより、乳入り飲食品の品質の安定性を高めることができる。
In a preferred embodiment of the present invention, the Lactobacillus paracasei is a dead bacterium.
Thereby, the stability of the quality of the food and drink containing milk can be enhanced.
本発明の好ましい形態では、前記乳入り飲食品のpHは5〜8である。
このような中性域の乳入り飲食品は、特に塩味を感じやすいためである。
In a preferred embodiment of the present invention, the milk-containing food or drink has a pH of 5 to 8.
This is because such foods and drinks containing milk in the neutral range are particularly susceptible to salty taste.
本発明の好ましい形態では、前記乳入り飲食品は、ナトリウムを38mg/100g以上含む。
このようなナトリウム濃度の乳入り飲食品は、特に塩味を感じやすいためである。
In a preferred embodiment of the present invention, the milk-containing food or drink contains 38 mg / 100 g or more of sodium.
This is because foods and drinks containing milk having such a sodium concentration are particularly susceptible to salty taste.
本発明の好ましい形態では、前記乳入り飲食品は、飲料である。
飲料は、特に塩味を感じやすいためである。
In a preferred embodiment of the present invention, the milk-containing food or drink is a beverage.
This is because beverages are particularly susceptible to salty taste.
本発明の一形態では、前記乳入り飲食品は、さらに、前記ラクトバチルス・パラカゼイ(Lactobacillus paracasei)とは異なる乳酸菌の生菌を含む。
また、本発明の一形態では、前記乳入り飲食品は、さらに、ビフィドバクテリウム属細菌を含む。
これらの細菌をさらに含むことで、栄養価が高く、風味に優れた乳入り飲食品を提供することができる。
In one embodiment of the invention, the milky food or drink further comprises a viable lactic acid bacterium that is different from the Lactobacillus paracasei.
Further, in one form of the present invention, the milk-containing food or drink further contains a bacterium of the genus Bifidobacterium.
By further containing these bacteria, it is possible to provide a milk-containing food or drink having high nutritional value and excellent flavor.
前記課題を解決する本発明は、ラクトバチルス・パラカゼイ(Lactobacillus paracasei)を塩味を有する乳入り飲食品に添加することを含む、塩味の低減方法である。
前記ラクトバチルス・パラカゼイ(Lactobacillus paracasei)の好ましい形態については、本発明の乳入り飲食品について述べた通りである。
The present invention that solves the above problems is a method for reducing saltiness, which comprises adding Lactobacillus paracasei to a salty milky food or drink.
The preferred form of Lactobacillus paracasei is as described for the milk-containing food and drink of the present invention.
前記課題を解決する本発明は、無脂乳固形分が9質量%以上である乳入り飲食品の製造方法であって、乳原料にラクトバチルス・パラカゼイ(Lactobacillus paracasei)を添加する工程を含み、乳原料由来のナトリウムを除去する工程を含まない、製造方法である。
これにより、膜処理などによるナトリウムの除去という工程を経ずとも、塩味を低減した、無脂乳固形分が高い乳入り飲食品を製造することが可能となる。
The present invention for solving the above problems is a method for producing a milk-containing food or drink having a non-fat milk solid content of 9% by mass or more, and includes a step of adding Lactobacillus paracasei to a dairy raw material. It is a manufacturing method that does not include a step of removing sodium derived from a dairy raw material.
As a result, it becomes possible to produce a milk-containing food or drink having a reduced salty taste and a high non-fat milk solid content without going through a step of removing sodium by a membrane treatment or the like.
本発明によれば、無脂乳固形分を多く含む乳入り飲食品の塩味を低減することができる。そして、コク等の高級感あるいは栄養価に優れ、かつ風味が良好な乳入り飲食品の提供が可能となる。また、本発明によれば、膜処理などによるナトリウムの除去という工程を経ずとも、塩味が低減された、無脂乳固形分が高い乳入り飲食品を製造することが可能となる。 According to the present invention, it is possible to reduce the salty taste of milk-containing foods and drinks containing a large amount of non-fat milk solids. Then, it becomes possible to provide foods and drinks containing milk having a high-class feeling such as richness or nutritional value and having a good flavor. Further, according to the present invention, it is possible to produce a milk-containing food or drink having a reduced salty taste and a high non-fat milk solid content without going through a step of removing sodium by a membrane treatment or the like.
次に、本発明の好ましい実施形態について詳細に説明する。ただし、本発明は以下の好ましい実施形態に限定されず、本発明の範囲内で自由に変更することができるものである。尚、本明細書において百分率は特に断りのない限り質量による表示である。 Next, a preferred embodiment of the present invention will be described in detail. However, the present invention is not limited to the following preferred embodiments, and can be freely changed within the scope of the present invention. In the present specification, the percentage is expressed by mass unless otherwise specified.
本明細書において、「乳入り飲食品」とは、乳原料を用いて加工した飲食品をいう。本発明の乳入り飲食品としては、好ましくは発酵乳、乳酸菌飲料、乳飲料が挙げられる。本発明の乳入り飲食品の原料のうち、乳原料が占める割合は、好ましくは20質量%以上、好ましくは25質量%以上、さらに好ましくは30質量%以上である。
中でも、飲料であることが好ましく、例えば、日本国の「乳及び乳製品の成分規格等に関する省令」(乳等省令)にいう乳酸菌飲料、乳飲料の他、乳等省令にいう発酵乳のうち液状のもの(液状発酵乳)が挙げられる。
また、本明細書において、「乳原料」とは、乳由来の原料をいい、乳等省令における乳製品を特に制限なく用いることができる。本発明に用いられる乳原料として、例えば、生乳、脱脂乳、脱脂濃縮乳、全粉乳、脱脂粉乳、クリーム、バター、バターオイル、チーズ、濃縮乳、無糖練乳、無糖脱脂練乳、加糖練乳、クリームパウダー、ホエイパウダー、バターミルクパウダー、加糖粉乳、発酵乳、発酵乳パウダー、ブロックミルク等が挙げられる。乳原料としてより好ましくは、生乳、脱脂乳、脱脂濃縮乳、全粉乳、脱脂粉乳、クリーム、バター、ホエイパウダーが用いられる。本発明の乳入り飲食品に用いる乳原料としては、これらを1種又は2種以上含むものが好適で、膜処理を行っていないものを含むことがより好ましい。ただし、膜処理を行った乳原料を用いることもできる。
In the present specification, "food and drink containing milk" means food and drink processed using dairy raw materials. The milk-containing food and drink of the present invention preferably includes fermented milk, lactic acid bacteria beverages, and milk beverages. The proportion of the raw material of the milk-containing food or drink of the present invention is preferably 20% by mass or more, preferably 25% by mass or more, and more preferably 30% by mass or more.
Among them, beverages are preferable, and for example, among lactic acid bacteria beverages and milk beverages referred to in Japan's "Ministry Ordinance on Ingredient Standards for Milk and Milk Products" (Ministry Ordinance for Milk, etc.), and fermented milk referred to in the Ordinance for Milk, etc. Examples include liquid milk (liquid fermented milk).
Further, in the present specification, the “milk raw material” refers to a raw material derived from milk, and dairy products specified in the Ordinance of the Ministry of Milk, etc. can be used without particular limitation. Examples of milk raw materials used in the present invention include raw milk, defatted milk, defatted concentrated milk, whole milk powder, defatted milk powder, cream, butter, butter oil, cheese, concentrated milk, unsweetened condensed milk, unsweetened defatted condensed milk, and sweetened condensed milk. Examples thereof include cream powder, whey powder, butter milk powder, sweetened milk powder, condensed milk, fermented milk powder, and block milk. More preferably, raw milk, skim milk, defatted concentrated milk, whole milk powder, skim milk powder, cream, butter, and whey powder are used as the dairy raw material. As the milk raw material used in the milk-containing food or drink of the present invention, those containing one or more of these are preferable, and those containing no membrane treatment are more preferable. However, a dairy raw material that has undergone membrane treatment can also be used.
本発明の乳入り飲食品には、本発明の効果を損なわない範囲内で、適宜、任意成分を用いることができる。当該任意成分として、例えば、油脂類(動植物性)、食物繊維、乳化剤、安定化剤、増粘剤、糖類、糖アルコール類、多糖類、pH調整剤、脂肪酸エステル類、矯味矯臭剤、香料、賦形剤等が挙げられる。 In the milk-containing food or drink of the present invention, any component can be appropriately used as long as the effect of the present invention is not impaired. Examples of the optional components include fats and oils (animal and vegetable), dietary fiber, emulsifiers, stabilizers, thickeners, sugars, sugar alcohols, polysaccharides, pH adjusters, fatty acid esters, flavoring agents, flavors, etc. Examples thereof include excipients.
本発明の乳入り飲食品における無脂乳固形分とは、乳脂肪以外の乳固形分のことを言う。無脂乳固形分は、ゲルベル法等により飲食品中の脂肪量を定量し、直接乾燥法により飲食品中の水分量を定量したのち、これらの定量値から以下の式に基づいて定量することができる。
無脂乳固形分=全固形分−乳脂肪分
本発明の乳入り飲食品における無脂乳固形分は、9質量%以上であり、好ましくは10質量%以上である。このような範囲の無脂乳固形分の乳入り飲食品において、塩味低減の課題が特に存在する。また、本発明の乳入り飲食品における無脂乳固形分の上限値は、物性、嗜好性等を考慮して決定され、好ましくは20質量%以下、さらに好ましくは15質量%以下である。
The non-fat milk solid content in the food and drink containing milk of the present invention refers to a milk solid content other than milk fat. The fat-free milk solid content shall be quantified by the Gerber method or the like, the amount of water in the food or drink by the direct drying method, and then quantified from these quantified values based on the following formula. Can be done.
Non-fat milk solid content = total solid content-milk fat content The non-fat milk solid content in the milk-containing food or drink of the present invention is 9% by mass or more, preferably 10% by mass or more. In foods and drinks containing milk with a non-fat milk solid content in such a range, there is a particular problem of reducing saltiness. The upper limit of the non-fat milk solid content in the milk-containing food or drink of the present invention is determined in consideration of physical properties, palatability, etc., and is preferably 20% by mass or less, more preferably 15% by mass or less.
本発明において、「塩味」とは、食塩(塩化ナトリウム)等の塩味を呈する物質を口に含んだときに感じる味をいう。 In the present invention, the "salty taste" refers to the taste felt when a substance exhibiting a salty taste such as salt (sodium chloride) is contained in the mouth.
本発明の乳入り飲食品におけるナトリウム濃度は、好ましくは38mg/100g以上であり、より好ましくは40mg/100g以上である。このような範囲のナトリウム濃度の乳入り飲食品において、塩味低減の課題が特に存在する。また、本発明の乳入り飲食品におけるナトリウム濃度の上限値は、嗜好性を考慮して決定され、好ましくは85mg/100g以下であり、さらに好ましくは80mg/100g以下であり、より好ましくは70mg/100g以下であり、よりさらに好ましくは65mg/100g以下である。
なお、ナトリウム濃度は、ICP発光分析法により定量することができる。
The sodium concentration in the milk-containing food or drink of the present invention is preferably 38 mg / 100 g or more, and more preferably 40 mg / 100 g or more. In milk-containing foods and drinks having a sodium concentration in such a range, there is a particular problem of reducing saltiness. The upper limit of the sodium concentration in the milk-containing food or drink of the present invention is determined in consideration of palatability, and is preferably 85 mg / 100 g or less, more preferably 80 mg / 100 g or less, and more preferably 70 mg / g. It is 100 g or less, and more preferably 65 mg / 100 g or less.
The sodium concentration can be quantified by ICP emission spectrometry.
本発明の乳入り飲食品のpHは特に制限されないが、好ましくは5〜8、さらに好ましくは6.5〜8である。中性域の乳入り飲食品は、酸性域の乳入り飲食品に対して相対的に塩味低減の課題が大きいためである。 The pH of the milk-containing food or drink of the present invention is not particularly limited, but is preferably 5 to 8, and more preferably 6.5 to 8. This is because the foods and drinks containing milk in the neutral region have a greater problem of reducing saltiness than the foods and drinks containing milk in the acidic region.
本発明の乳入り飲食品は、膜処理を行わずに製造されたものであってもよい。具体的には、ナノフィルトレーション(NF)膜等による乳原料からのナトリウムの除去を行わずに製造したものであってもよい。これにより、低分子のミネラルや香気成分を製品に保持することができる。
ただし、本発明の乳入り飲食品に用いられる乳原料の一部又は全部を膜処理することが制限されるものではなく、最終的な飲食品の形態を考慮して、適宜乳原料を膜処理することもできる。
The food or drink containing milk of the present invention may be produced without performing a membrane treatment. Specifically, it may be produced without removing sodium from the milk raw material by a nanofiltration (NF) film or the like. As a result, low-molecular-weight minerals and aroma components can be retained in the product.
However, it is not limited to film-treat a part or all of the milk raw material used for the milk-containing food or drink of the present invention, and the milk raw material is appropriately film-treated in consideration of the final form of the food or drink. You can also do it.
本発明の乳入り飲食品は、ラクトバチルス・パラカゼイ(Lactobacillus paracasei)に属する細菌(以下、単に「ラクトバチルス・パラカゼイ」ともいう。)を含む。ラクトバチルス・パラカゼイとしては、例えば、ラクトバチルス・パラカゼイのMCC1849株(NITE BP−01633)、MCC1375株(FERM BP−11313)、SBT−2558株、SBT0327(NITE ABP−1129)、SBT2105(NITE P−1130)、SBT2203(NITE ABP−1131)、SBT2215(NITE ABP−1132)、SBT11408(NITE ABP−1133)、ATCC25598、OLL204220株(NITE BP−02244)、K71株(FERM BP−11098)等が挙げられる。
ラクトバチルス・パラカゼイは、同細菌の菌体を含む培養物の形態で含まれていてもよく、培養物から回収した菌体の形態で含まれていてもよい。また、前述の培養物は、加熱乾燥物、凍結乾燥物等の乾燥物の形態で含まれていてもよい。
The milk-containing food or drink of the present invention contains bacteria belonging to Lactobacillus paracasei (hereinafter, also simply referred to as "Lactobacillus paracasei"). Examples of the lactobacillus paracasei include MCC1849 strain (NITE BP-01633), MCC1375 strain (FERM BP-11313), SBT-2558 strain, SBT0327 (NITE ABP-1129), and SBT2105 (NITE BP-1129) of lactobacillus paracasei. 1130), SBT2203 (NITE ABP-1131), SBT2215 (NITE ABP-1132), SBT11408 (NITE ABP-1133), ATCC25598, OLL204220 strain (NITE BP-02244), K71 strain (FERM BP-110), etc. ..
Lactobacillus paracasei may be contained in the form of a culture containing the cells of the same bacterium, or may be contained in the form of cells recovered from the culture. In addition, the above-mentioned culture may be contained in the form of a dried product such as a heat-dried product or a freeze-dried product.
ラクトバチルス・パラカゼイの培養方法は特に限定されず、乳酸菌の培養に通常用いられる方法を適宜用いることができる。例えば、培養温度は25〜50℃でよく、35〜42℃であることが好ましい。また培養は好気条件下、及び嫌気条件下のいずれで行ってもよいが、嫌気条件下で行うことが好ましく、例えば、炭酸ガス等の嫌気ガスを通気しながら培養することができる。また、液体静置培養等の微好気条件下で培養してもよい。 The method for culturing Lactobacillus paracasei is not particularly limited, and a method usually used for culturing lactic acid bacteria can be appropriately used. For example, the culture temperature may be 25 to 50 ° C, preferably 35 to 42 ° C. Further, the culture may be carried out under either aerobic conditions or anaerobic conditions, but it is preferably carried out under anaerobic conditions, and for example, the culture can be carried out while aerating an anaerobic gas such as carbon dioxide gas. Further, the cells may be cultured under microaerobic conditions such as liquid static culture.
ラクトバチルス・パラカゼイを培養する培地としては、特に限定されず、乳酸菌の培養に通常用いられる培地を適宜用いることができる。すなわち、炭素源としては、例えば、ガラクトース、グルコース、フルクトース、マンノース、セロビオース、マルトース、ラクトース、スクロース、トレハロース、デンプン、デンプン加水分解物、廃糖蜜等の糖類を資化性に応じて使用できる。窒素源としては、例えば、アンモニア、硫酸アンモニウム、塩化アンモニウム、硝酸アンモニウムなどのアンモニウム塩類や硝酸塩類を使用できる。また、無機塩類としては、例えば、塩化ナトリウム、塩化カリウム、リン酸カリウム、硫酸マグネシウム、塩化カルシウム、硝酸カルシウム、塩化マンガン、硫酸第一鉄等を用いることができる。また、ペプトン、大豆粉、脱脂大豆粕、肉エキス、酵母エキス等の有機成分を用いてもよい。また、調製済みの培地としては、例えばMRS培地を好適に用いることができる。 The medium for culturing Lactobacillus paracasei is not particularly limited, and a medium usually used for culturing lactic acid bacteria can be appropriately used. That is, as the carbon source, for example, saccharides such as galactose, glucose, fructose, mannose, cellobiose, maltose, lactose, sucrose, trehalose, starch, starch hydrolysate, and waste sugar honey can be used depending on the assimilation property. As the nitrogen source, for example, ammonium salts such as ammonia, ammonium sulfate, ammonium chloride, and ammonium nitrate, and nitrates can be used. Further, as the inorganic salts, for example, sodium chloride, potassium chloride, potassium phosphate, magnesium sulfate, calcium chloride, calcium nitrate, manganese chloride, ferrous sulfate and the like can be used. In addition, organic components such as peptone, soybean flour, defatted soybean meal, meat extract, and yeast extract may be used. Further, as the prepared medium, for example, MRS medium can be preferably used.
本発明においては、ラクトバチルス・パラカゼイは、生菌であっても死菌であってもよいが、死菌であることが好ましい。
死菌を用いることで、乳入り飲食品の品質管理が容易となる。
In the present invention, Lactobacillus paracasei may be a live bacterium or a dead bacterium, but is preferably a dead bacterium.
By using killed bacteria, quality control of milk-containing foods and drinks becomes easy.
ラクトバチルス・パラカゼイの死菌は、培養物を加熱処理することなどで得ることができる。 Killed bacteria of Lactobacillus paracasei can be obtained by heat-treating the culture.
ラクトバチルス・パラカゼイとして、具体的には、ラクトバチルス・パラカゼイMCC1849(NITE BP−01633)株が挙げられる。MCC1849株は、2013年6月6日に、独立行政法人製品評価技術基盤機構特許微生物寄託センター(〒292−0818千葉県木更津市かずさ鎌足2−5−8 122号室)に、NITE P−01633の受託番号で寄託され、2014年1月31日にブダペスト条約に基づく国際寄託に移管され、NITE BP−01633の受託番号が付与されている。 Specific examples of Lactobacillus paracasei include the Lactobacillus paracasei MCC1849 (NITE BP-01633) strain. The MCC1849 strain was transferred to NITE P-01633 on June 6, 2013 at the National Institute of Technology and Evaluation Patent Microorganisms Depositary Center (Room 2-5-8 122, Kazusakamatari, Kisarazu City, Chiba Prefecture 292-0818). It was deposited with the deposit number of NITE BP-01633, which was transferred to the international deposit based on the Budapest Treaty on January 31, 2014, and has been given the deposit number of NITE BP-01633.
また、ラクトバチルス・パラカゼイとしては、上記菌株に制限されず、同菌株と実質的に同等の菌株であってもよい。実質的に同等の菌株とは、ラクトバチルス・パラカゼイに属する菌株であって、寄託菌株と同程度の塩味低減作用を有する菌株を言う。また、実質的に同等の菌株は、さらに、その16S rRNA遺伝子の塩基配列が、上記寄託菌株の16S rRNA遺伝子の塩基配列と98%以上、好ましくは99%以上、より好ましくは100%の相同性を有し、且つ、好ましくは上記寄託菌株と同一の菌学的性質を有する。さらに、本発明におけるラクトバチルス・パラカゼイは、本発明の効果が損なわれない限り、寄託菌株、又はそれと実質的に同等の菌株から、変異処理、遺伝子組換え、自然変異株の選択等によって育種された菌株であってもよい。 Further, the Lactobacillus paracasei is not limited to the above-mentioned strain, and may be a strain substantially equivalent to the same strain. A substantially equivalent strain is a strain belonging to Lactobacillus paracasei and has a salty taste reducing action comparable to that of a deposited strain. Further, the substantially equivalent strain has a homology in which the base sequence of the 16S rRNA gene is 98% or more, preferably 99% or more, more preferably 100% with the base sequence of the 16S rRNA gene of the depositary strain. And preferably have the same mycological properties as the above-mentioned deposited strain. Further, the Lactobacillus paracasei in the present invention is bred from a deposited strain or a strain substantially equivalent thereto by mutation treatment, genetic recombination, selection of a natural mutant strain, etc., as long as the effect of the present invention is not impaired. It may be a strain.
本発明の乳入り飲食品におけるラクトバチルス・パラカゼイの含有量は、下限値として好ましくは2.5×109cfu/100g以上、さらに好ましくは5×109cfu/100g以上であり、上限値としては、好ましくは1×1011cfu/100g以下、さらに好ましくは5×1010cfu/100g以下が好ましい。
また、より好ましい当該数値範囲としては、好ましくは2.5×109〜1×1011cfu/100g、さらに好ましくは2.5×109〜5×1010cfu/100gである。ここで、ラクトバチルス・パラカゼイが死菌の場合の好ましい含有量は、前記「cfu」は、個(細胞の数)と置き換えることができる(以下の菌の含有量の説明においても同じ)。なお、cfuはColony forming unitを示す。
The content of lactobacillus paracasei in the milk-containing food or drink of the present invention is preferably 2.5 × 10 9 cfu / 100 g or more as a lower limit value, more preferably 5 × 10 9 cfu / 100 g or more as an upper limit value. Is preferably 1 × 10 11 cfu / 100 g or less, and more preferably 5 × 10 10 cfu / 100 g or less.
The numerical range is more preferably 2.5 × 10 9 to 1 × 10 11 cfu / 100 g, and further preferably 2.5 × 10 9 to 5 × 10 10 cfu / 100 g. Here, in the preferred content when Lactobacillus paracasei is a dead bacterium, the "cfu" can be replaced with an individual (number of cells) (the same applies to the following description of the bacterium content). In addition, cfu indicates Colony forming unit.
本発明の乳入り飲食品におけるラクトバチルス・パラカゼイの含有量は、無脂乳固形分1gに対し、下限値として好ましくは2.5×108cfu/g以上、さらに好ましくは3×108cfu/g以上であり、上限値としては、好ましくは6.8×109cfu/g以下、さらに好ましくは3.4×109cfu/g以下が好ましい。
また、より好ましい当該数値範囲として、好ましくは2.5×108〜6.8×109cfu/g、さらに好ましくは2.5×108〜3.4×109cfu/gである。
また、本発明の乳入り飲食品におけるラクトバチルス・パラカゼイの含有量は、ナトリウム1mgに対し、下限値として好ましくは5.5×107cfu/mg以上、さらに好ましくは7.6×107cfu/mg以上であり、上限値としては、好ましくは1.6×109cfu/mg以下、さらに好ましくは8×108cfu/mg以下が好ましい。
また、より好ましい当該数値範囲として、好ましくは5.5×107〜1.6×109cfu/mg、さらに好ましくは5.5×107〜8×108cfu/mgである。
The content of lactobacillus paracasei in the milk-containing food or drink of the present invention is preferably 2.5 × 10 8 cfu / g or more, more preferably 3 × 10 8 cfu, as a lower limit value with respect to 1 g of non-fat milk solids. It is / g or more, and the upper limit value is preferably 6.8 × 10 9 cfu / g or less, more preferably 3.4 × 10 9 cfu / g or less.
Further, the numerical value range is more preferably 2.5 × 10 8 to 6.8 × 10 9 cfu / g, and further preferably 2.5 × 10 8 to 3.4 × 10 9 cfu / g.
The content of lactobacillus paracasei in the milk-containing food or drink of the present invention is preferably 5.5 × 10 7 cfu / mg or more, more preferably 7.6 × 10 7 cfu, as a lower limit value with respect to 1 mg of sodium. It is / mg or more, and the upper limit value is preferably 1.6 × 10 9 cfu / mg or less, more preferably 8 × 10 8 cfu / mg or less.
The numerical range is more preferably 5.5 × 10 7 to 1.6 × 10 9 cfu / mg, and even more preferably 5.5 × 10 7 to 8 × 10 8 cfu / mg.
本発明の乳入り飲食品は、さらに、前述したラクトバチルス・パラカゼイ以外の種類の他の乳酸菌の生菌を含んでいてもよい。
他の乳酸菌としては、発酵乳、乳酸菌飲料に用いられているものを特に制限なく用いることができる。
他の乳酸菌の含有量は、製品の形態に合わせて、通常の範囲で選択することが可能である。
The milk-containing food or drink of the present invention may further contain viable bacteria of other types of lactic acid bacteria other than the above-mentioned Lactobacillus paracasei.
As other lactic acid bacteria, those used in fermented milk and lactic acid bacteria beverages can be used without particular limitation.
The content of other lactic acid bacteria can be selected in the usual range according to the form of the product.
本発明の乳入り飲食品は、さらにビフィドバクテリウム(Bifidobacterium)属に属する細菌の生菌を含んでいてもよい。
ビフィドバクテリウム属に属する細菌としては、発酵乳、乳酸菌飲料に用いられているものを特に制限なく用いることができ、例えば、ビフィドバクテリウム・ロンガム(Bifidobacterium longum)が好ましく挙げられる。
ビフィドバクテリウム属に属する細菌の含有量は、製品の形態に合わせて、通常の範囲で選択することが可能である。
The milk-containing food or drink of the present invention may further contain viable bacteria belonging to the genus Bifidobacterium.
As the bacterium belonging to the genus Bifidobacterium, those used in fermented milk and lactic acid bacteria beverages can be used without particular limitation, and for example, Bifidobacterium longum is preferable.
The content of bacteria belonging to the genus Bifidobacterium can be selected in the usual range according to the form of the product.
本発明の乳入り飲食品は、ラクトバチルス・パラカゼイを配合することを除いて、特に制限されず、通常の方法で製造することができる。
乳入り飲食品における無脂乳固形分は、乳原料として粉乳や濃縮乳を用いる方法、又は乳原料として用いる乳や乳製品の水分を除去し、無脂乳固形分を濃縮する方法によって調整することができる。
ここで、本発明の製造方法では、好ましくは乳原料由来のナトリウム除去を行わなくてもよい。より具体的には、ナトリウムを除去しうる膜処理を行わなくてもよい。これにより、工程全体を簡便にすることが可能となる。また、膜処理を行わないことで、ミネラルや香気成分を製品中に保持することができる。
The food and drink containing milk of the present invention is not particularly limited except that it contains Lactobacillus paracasei, and can be produced by a usual method.
The non-fat milk solids in milk-containing foods and drinks are adjusted by a method of using powdered milk or concentrated milk as a dairy raw material, or a method of removing water from milk or dairy products used as a dairy raw material and concentrating the non-fat milk solids. be able to.
Here, in the production method of the present invention, it is not necessary to remove sodium derived from the milk raw material. More specifically, it is not necessary to perform a membrane treatment capable of removing sodium. This makes it possible to simplify the entire process. In addition, minerals and aroma components can be retained in the product by not performing the film treatment.
発酵乳や乳酸菌飲料の製造においては、前記他の乳酸菌を培養したスターターを用いて、乳原料を発酵する過程において、発酵前又は発酵後の任意の時点でラクトバチルス・パラカゼイを添加することができる。 In the production of fermented milk and lactic acid bacteria beverages, Lactobacillus paracasei can be added at any time before or after fermentation in the process of fermenting the dairy raw material using the starter in which the other lactic acid bacteria are cultured. ..
また、本発明は、ラクトバチルス・パラカゼイを塩味を有する乳入り飲食品に添加することを含む、塩味の低減方法である。
乳入り飲食品、及びラクトバチルス・パラカゼイの好ましい形態については、前述の通りである。同方法に用いられるラクトバチルス・パラカゼイの形態として、例えば、ラクトバチルス・パラカゼイの培養物の加熱乾燥物、及び凍結乾燥物が好ましく挙げられる。
この観点から、本発明はまた、ラクトバチルス・パラカゼイの培養物の加熱乾燥物又は凍結乾燥物を有効成分とする、乳入り飲食品の塩味低減剤を提供する。
The present invention is also a method for reducing saltiness, which comprises adding Lactobacillus paracasei to a salty milky food or drink.
The preferred forms of milk-containing foods and drinks and Lactobacillus paracasei are as described above. As the form of Lactobacillus paracasei used in the same method, for example, a heat-dried product of a culture of Lactobacillus paracasei and a freeze-dried product are preferably mentioned.
From this point of view, the present invention also provides a salty taste reducing agent for milk-containing foods and drinks, which comprises a heat-dried product or a freeze-dried product of a culture of Lactobacillus paracasei as an active ingredient.
以下に実施例を用いて本発明をさらに詳しく説明するが、本発明はこれら実施例に限定されるものではない。 Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited to these Examples.
(1)試験1
本試験では、ラクトバチルス・パラカゼイを種々の濃度で添加した場合の塩味低減作用を評価した。
本試験で用いるラクトバチルス・パラカゼイMCC1849株は死菌体の凍結乾燥物(実施例においてMCC1849培養物という)であり、菌数(細胞の数)は凍結乾燥物の規格に応じて、任意の数となるように配合量を適宜変更することができる。本試験では凍結乾燥物1g当たり2500億個の菌数の凍結乾燥物を用いた。
なお、ラクトバチルス・パラカゼイMCC1849株の凍結乾燥物は森永乳業株式会社より入手できる。
(1) Test 1
In this test, the salty taste reducing effect when Lactobacillus paracasei was added at various concentrations was evaluated.
The Lactobacillus paracasei MCC1849 strain used in this test is a lyophilized product of dead cells (referred to as MCC1849 culture in the examples), and the number of bacteria (number of cells) is arbitrary according to the specifications of the lyophilized product. The blending amount can be appropriately changed so as to be. In this test, a freeze-dried product having 250 billion bacteria per gram of the freeze-dried product was used.
The freeze-dried product of Lactobacillus paracasei MCC1849 strain can be obtained from Morinaga Milk Industry Co., Ltd.
表1に示す処方に従って、各成分を溶解水(精製水)に溶解させ全量を4000mLとした。前記溶解液を85℃で6分加温保持後、15MPaで均質化、130℃で2秒間加熱殺菌し、ペットボトルに充填した比較例1及び実施例1〜4の液体飲料を得た。
このときの実施例1〜4の配合設計はラクトバチルス・パラカゼイ培養物を含む乳入り飲食品(飲料)(無脂乳固形分15質量%、乳脂肪分2.5質量%、pH6.8)であり、ナトリウム含有量は65mg/100gであり、MCC1849菌数は50億/100g〜500億/100gであった。
また、比較例1としてラクトバチルス・パラカゼイ培養物を含まない乳入り飲食品(飲料)を製造した。
なお、表中の乳原料は、膜処理などの脱塩処理を行っていない乳由来の原料であり、すべての例で同じものを用いた。
According to the formulation shown in Table 1, each component was dissolved in dissolved water (purified water) to make a total volume of 4000 mL. The solution was heated and held at 85 ° C. for 6 minutes, homogenized at 15 MPa, and sterilized by heating at 130 ° C. for 2 seconds to obtain liquid beverages of Comparative Example 1 and Examples 1 to 4 filled in PET bottles.
The formulation design of Examples 1 to 4 at this time is a food or drink (beverage) containing milk containing a Lactobacillus paracasei culture (non-fat milk solid content 15% by mass, milk fat content 2.5% by mass, pH 6.8). The sodium content was 65 mg / 100 g, and the number of MCC1849 bacteria was 5 billion / 100 g to 50 billion / 100 g.
In addition, as Comparative Example 1, a milk-containing food or drink (beverage) containing no Lactobacillus paracasei culture was produced.
The milk raw material in the table is a milk-derived raw material that has not been desalted such as a membrane treatment, and the same raw material was used in all the examples.
製造した乳入り飲食品を、乳入り飲食品の評価の専門パネラー7名によって試飲し、塩味の評価を行った。
評価は、以下の基準を用い、比較例と比較した場合にどの程度塩味を感じるかの相対評価とした。結果を表2に示す。
<基準>
5:塩味が弱い
4:塩味がやや弱い
3:塩味が比較例と変わらない
2:塩味がやや強い
1:塩味が強い
The produced milk-containing foods and drinks were tasted by seven panelists specializing in the evaluation of milk-containing foods and drinks, and the salty taste was evaluated.
The evaluation was a relative evaluation of how much salty taste was felt when compared with the comparative example using the following criteria. The results are shown in Table 2.
<Criteria>
5: Weak salty taste 4: Slightly weak salty taste 3: Same as the comparative example 2: Slightly strong salty taste 1: Strong salty taste
表2に示す通り、MCC1849培養物を添加した実施例の乳入り飲食品は、ラクトバチルス・パラカゼイの塩味低減効果が容量依存的に増大し、比較例1と比較して、実施例1〜4は顕著に塩味の低減作用が得られた。これにより、ラクトバチルス・パラカゼイの塩味の低減作用を十分に得るためには、無脂乳固形分1gあたりのラクトバチルス・パラカゼイの含有量が、3億(3×108)個以上が好ましいことが分かった。
ここで、無脂乳固形分が15質量%の乳入り飲食品100g中には、約65mgのナトリウムが含まれる。そのため、ナトリウムとの関係で見ると、ナトリウム1mgあたりのラクトバチルス・パラカゼイの含有量が、7600万(7.6×107)個以上であることが好ましいことが分かった。
As shown in Table 2, in the milk-containing foods and drinks of Examples to which the MCC1849 culture was added, the salty taste reducing effect of Lactobacillus paracasei increased in a volume-dependent manner, and Examples 1 to 4 were compared with Comparative Example 1. Remarkably reduced the salty taste. Thus, in order to sufficiently obtain the effect of reducing the salty taste of Lactobacillus paracasei, the content of Lactobacillus paracasei per non-fat milk solids 1g is 300 million (3 × 10 8) or more is preferable I found out.
Here, about 65 mg of sodium is contained in 100 g of a milk-containing food or drink having a non-fat milk solid content of 15% by mass. Therefore, when viewed in relation to the sodium content of Lactobacillus paracasei per sodium 1 mg, it was found that it is preferable that 76 million (7.6 × 10 7) or greater.
(2)試験2
表3に示す配合で、各成分を溶解水(精製水)に溶解させ全量を4000mLとした。前記溶解液を85℃で6分加温保持後、15MPaで均質化、130℃で2秒間加熱殺菌し、ペットボトルに充填した実施例5及び参考例、比較例2及び3の液体飲料(pH6.8)を得た。
得られた液体飲料を用いて、試験1と同様に塩味を評価した。比較例2と実施例5の間、及び比較例3と参考例との間では同じ乳原料を用いた。ここで、乳原料は、膜処理などの脱塩処理を行っていないものを用いた。
実施例5は比較例2との相対評価、参考例は比較例3との相対評価とした。
(2) Test 2
With the formulation shown in Table 3, each component was dissolved in dissolved water (purified water) to make a total volume of 4000 mL. The liquid beverage (pH 6) of Example 5 and Reference Examples, Comparative Examples 2 and 3 filled in a PET bottle after homogenizing the solution at 85 ° C. for 6 minutes, homogenizing it at 15 MPa, and sterilizing it by heating at 130 ° C. for 2 seconds. 8.8) was obtained.
Using the obtained liquid beverage, the salty taste was evaluated in the same manner as in Test 1. The same dairy ingredients were used between Comparative Example 2 and Example 5, and between Comparative Example 3 and Reference Example. Here, as the dairy raw material, one that has not been desalted such as a membrane treatment was used.
Example 5 was evaluated relative to Comparative Example 2, and Reference Example was evaluated relative to Comparative Example 3.
実施例5の無脂乳固形分含量が10質量%の乳入り飲食品では、ラクトバチルス・パラカゼイの塩味低減効果が得られた。一方、参考例の無脂乳固形分含量が8質量%の乳入り飲食品では、もともと塩味を感じにくいことから、ラクトバチルス・パラカゼイの塩味低減効果が限定的であった。これより、ラクトバチルス・パラカゼイの塩味低減効果は、無脂乳固形分含量が9質量%以上、より好ましくは10質量%以上の場合に、特に顕著であることがわかった。 In the milk-containing food or drink having a non-fat milk solid content of 10% by mass in Example 5, the salty taste reducing effect of Lactobacillus paracasei was obtained. On the other hand, in the food and drink containing milk having a non-fat milk solid content of 8% by mass in the reference example, the salty taste was originally difficult to be felt, so that the salty taste reducing effect of Lactobacillus paracasei was limited. From this, it was found that the salty taste reducing effect of Lactobacillus paracasei was particularly remarkable when the non-fat milk solid content content was 9% by mass or more, more preferably 10% by mass or more.
ここで、無脂乳固形分が10質量%の乳入り飲食品100g中には、約45mgのナトリウムが含まれ、無脂乳固形分が9質量%の乳入り飲食品100g中には、約40mgのナトリウムが含まれ、無脂乳固形分含量が8質量%の乳入り飲食品100g中には、約35mgのナトリウムが含まれる。そのため、乳入り飲食品100gあたりのナトリウム濃度が38mg以上、さらにナトリウム濃度が40mg以上、よりさらに、ナトリウム濃度が45mg以上の場合にラクトバチルス・パラカゼイの塩味低減効果が顕著に得られることがわかった。
また、実施例5の結果から、ラクトバチルス・パラカゼイの塩味の低減作用を十分に得るためには、無脂乳固形分1gあたりのラクトバチルス・パラカゼイの含有量が、2.5億(2.5×108)個以上が好ましいことが分かった。
ここで、無脂乳固形分が10質量%の乳入り飲食品100g中には、約45mgのナトリウムが含まれる。そのため、ナトリウムとの関係で見ると、ナトリウム1mgあたりのラクトバチルス・パラカゼイの含有量が、5500万(5.5×107)個以上であることが好ましいことが分かった。
Here, about 45 mg of sodium is contained in 100 g of a milk-containing food or drink having a non-fat milk solid content of 10% by mass, and about 45 mg of sodium is contained in 100 g of a milk-containing food or drink having a non-fat milk solid content of 9% by mass. About 35 mg of sodium is contained in 100 g of a milk-containing food or drink containing 40 mg of sodium and having a non-fat milk solid content of 8% by mass. Therefore, it was found that the salty taste reducing effect of Lactobacillus paracasei can be remarkably obtained when the sodium concentration per 100 g of milk-containing food or drink is 38 mg or more, the sodium concentration is 40 mg or more, and the sodium concentration is 45 mg or more. ..
Further, from the results of Example 5, in order to sufficiently obtain the salty taste-reducing effect of Lactobacillus paracasei, the content of Lactobacillus paracasei per 1 g of non-fat milk solids is 250 million (2. It was found that 5 × 10 8 ) or more was preferable.
Here, about 45 mg of sodium is contained in 100 g of a milk-containing food or drink having a non-fat milk solid content of 10% by mass. Therefore, when viewed in relation to the sodium content of Lactobacillus paracasei per sodium 1 mg, it was found that it is preferable that 55 million (5.5 × 10 7) or greater.
(3)試験3
表5に示す配合で、乳原料及びラクトバチルス・パラカゼイMCC1849培養物(MCC1849培養物)を溶解水(精製水)に溶解させ全量を4000mLとした。前記溶解液を85℃で6分加温保持後、15MPaで均質化、130℃で2秒間加熱殺菌し、ラクトバチルス・パラカゼイ以外の種類の他の乳酸菌の生菌(乳酸菌(生菌)培養物)及びビフィドバクテリウム・ロンガム(Bifidobacterium longum)BB536株(BB536(生菌)培養物)を添加、混合させた後、ペットボトルに充填した比較例4及び実施例6の液体飲料を得た。
このときの実施例6の配合設計はラクトバチルス・パラカゼイ培養物を含む実施例の乳入り飲食品(飲料)(無脂乳固形分15質量%、乳脂肪分2.5質量%、pH6.8)であり、ナトリウム含有量は65mg/100gであり、MCC1849菌数は50億/100gであった。
また、比較例4としてラクトバチルス・パラカゼイ培養物を含まない乳入り飲食品(飲料)を製造した。
なお、表中の乳原料は、膜処理などの脱塩処理を行っていない乳由来の原料であり、すべての例で同じものを用いた。
(3) Test 3
With the formulation shown in Table 5, the dairy raw material and the Lactobacillus paracasei MCC1849 culture (MCC1849 culture) were dissolved in dissolved water (purified water) to make a total volume of 4000 mL. The lysate is heated and held at 85 ° C. for 6 minutes, homogenized at 15 MPa, sterilized by heating at 130 ° C. for 2 seconds, and viable bacteria (lactic acid bacteria (live bacteria) culture) of other types of lactic acid bacteria other than Lactobacillus paracasei. ) And Bifidobacterium longum BB536 strain (BB536 (live bacterium) culture) were added and mixed, and then the liquid beverages of Comparative Example 4 and Example 6 filled in PET bottles were obtained.
At this time, the compounding design of Example 6 was the milk-containing food or drink (beverage) of Example containing the Lactobacillus paracasei culture (non-fat milk solid content 15% by mass, milk fat content 2.5% by mass, pH 6.8). ), The sodium content was 65 mg / 100 g, and the number of MCC1849 bacteria was 5 billion / 100 g.
In addition, as Comparative Example 4, a milk-containing food or drink (beverage) containing no Lactobacillus paracasei culture was produced.
The milk raw material in the table is a milk-derived raw material that has not been desalted such as a membrane treatment, and the same raw material was used in all the examples.
乳酸菌(生菌)やビフィドバクテリウム属細菌(生菌)を含む形態の乳入り飲食品(乳酸菌飲料)においても、ラクトバチルス・パラカゼイの塩味低減効果が得られた。 The salty taste-reducing effect of Lactobacillus paracasei was also obtained in milk-containing foods and drinks (lactic acid bacteria beverages) containing lactic acid bacteria (live bacteria) and bifidobacteria (live bacteria).
本発明は、栄養価の高い乳入り飲食品の製造に有用である。 The present invention is useful for producing highly nutritious milk-containing foods and drinks.
NITE BP−01633 NITE BP-01633
Claims (12)
乳原料にラクトバチルス・パラカゼイ(Lactobacillus paracasei)に属する細菌を添加する工程を含み、
乳原料由来のナトリウムを除去する工程を含まない、製造方法。
A method for producing a food or drink containing milk having a non-fat milk solid content of 9% by mass or more.
Including the step of adding bacteria belonging to Lactobacillus paracasei to the dairy raw material.
A manufacturing method that does not include the step of removing sodium derived from dairy ingredients.
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CN114586850A (en) * | 2022-03-16 | 2022-06-07 | 黑龙江飞鹤乳业有限公司 | Milk-containing solid molded product containing cheese and preparation method thereof |
CN114668047A (en) * | 2022-04-18 | 2022-06-28 | 黑龙江飞鹤乳业有限公司 | Milk product and method for producing same |
WO2024004139A1 (en) * | 2022-06-30 | 2024-01-04 | 森永乳業株式会社 | Fermented milk and production method for fermented milk |
WO2024004138A1 (en) * | 2022-06-30 | 2024-01-04 | 森永乳業株式会社 | Beverage and production method for beverage |
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JP5646124B1 (en) * | 2013-07-12 | 2014-12-24 | 森永乳業株式会社 | Novel lactic acid bacteria and pharmaceuticals, foods and drinks, and feeds containing the novel lactic acid bacteria |
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Cited By (6)
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CN114586850A (en) * | 2022-03-16 | 2022-06-07 | 黑龙江飞鹤乳业有限公司 | Milk-containing solid molded product containing cheese and preparation method thereof |
CN114586850B (en) * | 2022-03-16 | 2023-09-12 | 黑龙江飞鹤乳业有限公司 | Cheese-containing solid-state forming product containing cheese and preparation method thereof |
CN114668047A (en) * | 2022-04-18 | 2022-06-28 | 黑龙江飞鹤乳业有限公司 | Milk product and method for producing same |
CN114668047B (en) * | 2022-04-18 | 2023-12-22 | 黑龙江飞鹤乳业有限公司 | Milk product and preparation method thereof |
WO2024004139A1 (en) * | 2022-06-30 | 2024-01-04 | 森永乳業株式会社 | Fermented milk and production method for fermented milk |
WO2024004138A1 (en) * | 2022-06-30 | 2024-01-04 | 森永乳業株式会社 | Beverage and production method for beverage |
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