JP2020134262A - Measurement device - Google Patents

Abstract

To provide a measurement device that can simultaneously measure activity such as photosynthetic activity, respiratory activity or the like based on, for example, oxygen density and the like about a plurality of measurement samples.SOLUTION: A measurement device 1 comprises: a measurement block 2 that has a plurality of concave parts 10 serving as a sample measurement chamber; a chamber main body 3 that houses the measurement block 2; a chamber lid 4 that covers an upper opening of the chamber main body 3; an inner lid 5 that can slide and move vertically with respect to the chamber main body 3, in which a plurality of plug members 11 individually sealing the plurality of concave parts 10 of the measurement block 2 accompanied by the slide movement downward are provided in a lower surface; a gas introduction port 6A introducing arbitrary gas into a space S between the measurement block 2 and inner lid 5 within the chamber main body 3 and gas derivation port 6B deriving the arbitrary gas from the space S, in which the gas introduction and derivation ports are attached to the chamber main body 3; and a density measurement instrument 7 that can measure density of prescribed gas within each concave part 10 of the measurement block 2.SELECTED DRAWING: Figure 6

Description

The present invention relates to a measuring device that simultaneously measures information on the concentration of a predetermined gas in a plurality of sample measuring chambers, and more particularly to a measuring device that simultaneously measures an activity that can use a change in the information as an index.

Conventionally, various methods have been known for measuring the photosynthetic activity of plants. For example, there is known a method of measuring photosynthetic activity by irradiating a plant with an electromagnetic wave and measuring the phase and amplitude of the electromagnetic wave modulated by the plant (Patent Document 1). In addition, a method of measuring the photosynthetic activity of a plant using a chamber is also known. In this method, CO ₂ gas or the like is introduced into the chamber, the plant is irradiated with light, and an oxygen electrode is used in the chamber. The photosynthetic activity of plants is measured by measuring the oxygen concentration.

However, in the conventional method, only one measurement sample can be measured in one measurement, and the photosynthetic activity of many measurement samples cannot be measured at the same time. Therefore, when measuring the photosynthetic activity of a plurality of measurement samples using a conventional method, a great deal of time, labor, or cost is required. Therefore, it is important to provide a technique capable of measuring photosynthetic activity more efficiently. This also applies when measuring activities such as respiratory activity other than photosynthetic activity.

JP-A-2017-153406

The present invention has been made in view of the above problems, and for a plurality of measurement samples, for example, activities such as photosynthetic activity and respiratory activity are introduced into each sample measurement chamber after introducing a gas suitable for activity measurement into each sample measurement chamber. To provide a measuring device capable of simultaneously measuring information on a predetermined gas concentration such as gas concentration (for example, oxygen concentration, carbon dioxide concentration, etc.) or pH, etc., at the same time and with time. With the goal.

The present invention is a measuring device that simultaneously measures information on the concentration of a predetermined gas in a plurality of sample measuring chambers, and includes a measuring block having a plurality of recesses serving as a sample measuring chamber and a chamber accommodating the measuring block. A plurality of bodies, a chamber lid that closes the upper opening of the chamber body, and a plurality of recesses of the measurement block that can be slid up and down with respect to the chamber body and individually seal the plurality of recesses of the measurement block as the slide moves downward. From the gas inlet and the space, which are attached to the chamber body and are attached to the chamber body and introduce arbitrary gas into the space between the measurement block and the inner lid. It is characterized by including a gas outlet for drawing out an arbitrary gas and a measuring instrument capable of measuring information on the concentration of the predetermined gas in each recess of the measuring block.

In the measuring device of the present invention, it is preferable to further provide a fixing means for detachably fixing the inner lid to the chamber lid. Further, the fixing means includes at least one magnet provided on one of the inner lid and the chamber lid, and at least one metal plate provided on the other of the inner lid and the chamber lid so as to face the magnet. , Is more preferable.

Further, in the measuring device of the present invention, the inner lid further includes a plurality of sealing members individually provided around the plurality of plug members, and the plurality of sealing materials are measured by the plurality of plug members. It is preferable that when the plurality of recesses of the block are individually sealed, the openings of the plurality of recesses are individually sealed by being sandwiched between the inner lid and the measurement block.

Further, in the measuring device of the present invention, the measuring block closes a metal first plate in which a plurality of through holes are formed and an opening on one side of the plurality of through holes of the first plate. A second plate fixed to the first plate so as to form a plurality of recesses is provided, the second plate is capable of transmitting light, and the plurality of through holes are grouped in the first plate. It is preferable that a flow path through which the liquid passes is formed so as to surround the flow path, and a water supply port for supplying the liquid to the flow path and a drainage port for discharging the liquid from the flow path are provided.

Further, in the measuring apparatus of the present invention, it is preferable that a packing for sealing the opening on one side of the plurality of through holes is interposed between the first plate and the second plate.

Further, in the measuring device of the present invention, the measuring device is a measuring device capable of measuring information on a predetermined gas concentration such as gas concentration or pH, and the measuring device is a plurality of recesses of the measuring block. It is individually set on the bottom, whereby the information can be acquired for each measurement block. For example, when measuring the oxygen concentration, the measuring device is an oxygen concentration measuring device for measuring the oxygen concentration. Although not limiting the present invention, the oxygen concentration measuring device is individually set on the bottoms of a plurality of recesses of the measuring block, and a plurality of fluorescences having an intensity corresponding to the oxygen concentration are emitted by irradiation with excitation light. A fluorescent oxygen sensor, a plurality of light emitting units that individually irradiate the plurality of fluorescent oxygen sensors with excitation light, and a plurality of light receiving units that individually measure fluorescence emitted from the plurality of fluorescent oxygen sensors. It is preferable to prepare. Further, it is more preferable that the sample is housed in the plurality of recesses of the measurement block on the fluorescent oxygen sensor via a light-shielding sheet and / or a breathable buffer material.

According to the measuring device of the present invention, a plurality of recesses serving as a sample measurement chamber of a measuring block can be simultaneously replaced with an arbitrary gas, and a plurality of plug members can be used to individually seal the plurality of recesses. .. Therefore, for example, when measuring the photosynthetic activity of plants, algae, these cells (including cultured cells), etc. as a measurement sample, it is possible to replace all the recesses of the measurement block with CO _2- containing gas all at once. At the same time, all the recesses can be individually sealed at the same time. Therefore, by measuring information on the concentration of oxygen generated by photosynthesis of the measurement sample in each recess (such as the oxygen concentration in the recess or the pH that changes based on the oxygen concentration dissolved in the solution in the recess), The photosynthetic activity of a plurality of measurement samples can be measured at the same time. As a result, it is possible to measure efficiently especially when there are a large number of measurement samples, and the measurement time can be significantly shortened.

The information regarding the predetermined gas is not limited to the gas concentration and pH, and may be any information that changes according to the gas concentration. In addition, other activities that can be measured based on information on oxygen concentration or carbon dioxide concentration, such as respiratory activity of an organism or its cells (including cultured cells), can also be measured using the present invention. In addition, other activities that can be measured based on information on the concentration of a predetermined gas other than the oxygen concentration and the carbon dioxide concentration can also be measured by using the present invention.

It is an exploded perspective view of the measuring device. It is an exploded perspective view of the measurement block. It is a top view of the measuring apparatus in a state where a chamber lid and an inner lid are omitted. (A) is a perspective view of a chamber lid, and (B) is an exploded perspective view of an inner lid. It is a perspective view of the state which the chamber lid and the inner lid are attached to a chamber body. It is sectional drawing of the measuring apparatus along the X1-X1 line of FIG. 3 in the state which attached the chamber lid and the inner lid to the chamber body. It is a perspective view of the measuring apparatus after sliding the inner lid downward with the chamber lid and the inner lid attached to the chamber body. It is sectional drawing of the measuring apparatus along the X1-X1 line of FIG. 3 in the state of FIG. FIG. 7 is a perspective view of a measuring device in a state where the chamber lid is removed from the chamber body after the state of FIG. 7. It is a perspective view of the measuring apparatus in the state of measuring the photosynthetic activity. It is data which shows the measurement result of the photosynthetic activity. It is data which shows the measurement result of the photosynthetic activity.

The measuring apparatus of the present invention simultaneously and temporally measures information on the concentration of a predetermined gas such as gas concentration and pH in a plurality of sample measurement chambers, thereby changing the gas concentration, pH, etc. of a plurality of measurement samples. The activity that can be used as an index is measured at the same time. For example, by measuring the oxygen concentration, carbon dioxide concentration or pH in a plurality of sample measurement chambers simultaneously and over time, based on the information on the oxygen concentration such as photosynthetic activity and respiratory activity or the information on the carbon dioxide concentration for a plurality of measurement samples. The measured activity can be measured at the same time. The activity to be measured by the measuring device of the present invention is not limited to the activity measured based on the information on the oxygen concentration and the information on the carbon dioxide concentration, and the information on the concentration of a predetermined gas other than oxygen and carbon dioxide. Other activities measured based on are also included in the measurement target. In addition, the information regarding a predetermined gas is not limited to the gas concentration and pH, but also includes information that changes according to other gas concentrations. In the following, an example in which the photosynthetic activity of a plurality of plants is measured is described.

FIG. 1 is an exploded perspective view of the measuring device 1 of the present embodiment, and FIG. 6 shows an internal configuration of the measuring device 1 of the present embodiment. The measuring device 1 of the present embodiment includes a measuring block 2 having a plurality of recesses 10 serving as a sample measuring chamber, a chamber main body 3 for accommodating the measuring block 2, and a chamber lid 4 for closing the upper opening of the chamber main body 3. An inner lid 5 having a plurality of plug members 11 on the lower surface, which can slide up and down with respect to the chamber body 3 and individually seal a plurality of recesses 20 of the measurement block 2 as the measurement block 2 slides downward. Gas inlet 6A attached to the chamber body 3 and introducing an arbitrary gas (for example, CO ₂ containing gas) into the space S (shown in FIG. 6) between the measurement block 2 and the inner lid 5 in the chamber body 3. A concentration measuring device (for example, oxygen) capable of measuring the concentration of a predetermined gas (for example, oxygen) in each recess 10 of the measuring block 2 and the gas outlet 6B for deriving an arbitrary gas (for example, CO ₂ containing gas) from the space S. For example, an oxygen concentration measuring device) 7 is provided.

First, as shown in FIG. 1, the measurement block 2 has a rectangular outer shape and exhibits a plate-like body having a predetermined thickness. A plurality of recesses 10 having a predetermined depth are formed on the upper surface of the measurement block 2. The plurality of recesses 10 are regularly arranged at equal intervals in the vertical and horizontal directions. Each recess 10 is designed to have a size capable of accommodating a measurement sample (for example, a plant leaf) to be measured for photosynthetic activity or the like. The number of recesses 10 is not particularly limited, but is 24 in this embodiment.

In the present embodiment, the measurement block 2 is fixed to the first plate 21 in which a plurality of through holes 20 are formed and the second plate 21 as shown in FIGS. 2, 3 and 6 and the like. It is configured to include a plate 22 and a packing 23 interposed between the first plate 21 and the second plate 22.

The first plate 21 is made of metal in this embodiment, and is preferably formed of a metal having high thermal conductivity such as aluminum or stainless steel. The first plate 21 does not necessarily have to be made of metal, and may be made of, for example, glass or synthetic resin. Inside the first plate 21, a flow path 24 through which a liquid passes is formed so as to collectively surround a plurality of through holes 20. Further, on the end surface of the first plate 21, a water supply port 25 for introducing the liquid into the flow path 24 and a drain port 26 for drawing out the liquid from the flow path 24 are attached to the inlet end and the outlet end of the flow path 24, respectively. ing. For example, water is supplied to the flow path 24 from a tank, a water tank, or the like (not shown). The temperature of the water supplied to the flow path 24 is adjusted to a desired temperature, and the water set to the desired temperature flows through the flow path, so that the first plate 21 is maintained at a desired temperature. As a result, the temperature of the recess 10 serving as the sample measurement chamber can be set to a temperature suitable for the measurement conditions.

The second plate 22 is light permeable and is made entirely of, for example, a transparent or translucent material. The second plate 22 can be made of a synthetic resin such as acrylic or glass. The second plate 22 is fixed to the lower surface of the first plate 21 using screws (not shown) or the like so as to close the openings on one side (lower side in the drawing) of the plurality of through holes 20 of the first plate 21. ing. A plurality of the recesses 10 are formed by collectively closing the openings on one side of the plurality of through holes 20 of the first plate 21 with the second plate 22. As a result, each recess 10 can transmit light from the opening on the first plate 21 side to the bottom on the second plate 22 side.

The packing 23 is designed to be one size smaller than the first plate 21 and the second plate 22. The packing 23 is formed with a plurality of openings 25 that correspond one-to-one with the plurality of through holes 20 of the first plate 21. The packing 23 is arranged in contact with the lower surface of the first plate 21 so that the plurality of openings 27 individually overlap the plurality of through holes 20 of the first plate 21. As a result, as shown in FIG. 6, the openings on one side of the plurality of through holes 20 are collectively sealed by the packing 23. The packing 23 can be formed by using a known material conventionally used as a packing in order to prevent gas leakage. The packing 23 is sandwiched between the first plate 21 and the second plate 22 by fixing the second plate 22 to the lower surface of the first plate 21, and the second plate 22 is sandwiched by screws (not shown) or the like. It is fixed to the lower surface of the first plate 21 together with.

Next, as shown in FIGS. 1, 3, 5, and 6, the chamber body 3 has a bottom portion 30 having a rectangular outer shape, and a pair of front and rear side wall portions 31 and rear side walls that stand up on the peripheral edge of the bottom portion 30. It is configured to include a portion 32, a pair of left and right left wall portions 33, and a right side wall portion 34, and has a box shape with an open upper portion. In the present embodiment, the bottom portion 30 is fixed to the lower surface of a frame body composed of front, rear, left and right wall portions 31 to 34 by using screws (not shown) or the like.

On the upper surface of the bottom portion 30, positioning protrusions 35 are provided parallel to the wall portions 31 to 34 at the inner positions of the front, rear, left and right wall portions 31 to 34, respectively. The measurement block 2 is placed in the four protrusions 35.

Of the front, rear, left, and right wall portions 31 to 34 forming the frame, the front side wall portion 31 has a gas introduction port 6A for introducing gas into the chamber body 3, and the rear side wall portion 32 has gas introduced from inside the chamber body 3. The gas outlet 6B to be led out is attached to each. The gas introduction port 6A and the gas outlet 6B may be attached to any of the front, rear, left and right wall portions 31 to 34. A pipe 60 (shown in FIG. 3 or the like) is connected to the gas introduction port 6A, and an arbitrary gas such as a CO _2- containing gas is supplied from a cylinder or the like (not shown). A pipe 61 (shown in FIG. 3 or the like) is connected to the gas outlet 6B, and the gas in the chamber body 3 is discharged to the outside air, a tank, or the like.

Further, in the front side wall portion 31, a pipe 62 (shown in FIG. 3 and the like) connected to the water supply port 25 of the measurement block 2 housed in the chamber body 3 and a pipe 63 connected to the drain port 26 (shown in FIG. 3 and the like) Two insertion ports 36 through which (shown in FIG. 3 and the like) are individually inserted are formed at intervals from each other.

Of the front, rear, left, and right wall portions 31 to 34 forming the frame, the left wall portion 33 and the right wall portion 34 have a guide groove 37 having a concave shape cut out downward from the upper end in the central portion in the length direction. Are formed respectively. A pair of left and right first guide portions 51 of the inner lid 5, which will be described later, are inserted into the two guide grooves 37 from above and slide up and down.

Further, the left side wall portion 33 and the right side wall portion 34 are provided with protruding portions 38 protruding outward at positions below the guide grooves 37, respectively. A long hole-shaped guide hole 39 is formed in each of the two protrusions 38 at a boundary position between the left side wall portion 33 and the right side wall portion 34, respectively. A pair of left and right second guide portions 52 of the inner lid 5, which will be described later, are inserted into the two guide holes 39 from above and slide up and down.

Next, as shown in FIGS. 1, 4, 4 (A), 5, 6, and 8, the chamber lid 4 exhibits a plate-like body having a rectangular outer shape and a predetermined thickness. The chamber lid 4 is designed to be substantially the same size as the outer shape formed by the front, rear, left, and right wall portions 31 to 34 of the chamber body 3 so as to close the upper opening of the chamber body 3. When the chamber lid 4 covers the upper opening of the chamber body 3, it can be fixed to the chamber body 3 with screws (not shown) or the like. The chamber lid 4 may be made of metal, glass, or synthetic resin, but is preferably made of transparent synthetic resin such as acrylic.

Next, as shown in FIGS. 1, 4 (B) and 5 to 8, the inner lid 5 has a main body portion 50 having a rectangular outer shape and a pair of first first portions protruding from the left and right side surfaces of the main body portion 50. It is configured to include a guide portion 50 and a pair of second guide portions 51 provided so as to intersect each of the first guide portions 50 individually and vertically.

The main body 50 exhibits a plate-like body having a predetermined thickness. The main body 50 is designed to be one size smaller than the chamber lid 4, and is almost the same size as the upper opening so that it can be inserted into the chamber main body 3 through the upper opening of the chamber main body 3 and slid to move. Designed to size. On the lower surface of the main body 50, a plurality of plug members 11 for individually sealing the plurality of recesses 10 of the measurement block 2 when the chamber main body 3 is slid downward are provided.

The plurality of plug members 11 are regularly arranged on the lower surface of the main body 50 at equal intervals in the vertical and horizontal directions so as to correspond one-to-one with the recesses 10 of the measurement block 2. Each plug member 11 is designed to have a shape and size that fits tightly into the recess 10 of the measurement block 2, and may be formed of a known flexible or elastic material such as rubber or silicon. it can.

On the lower surface of the main body 50, a plurality of seal members 12 individually provided around the plurality of plug members 11 are provided. As shown in FIG. 8, the plurality of sealing materials 12 are sandwiched between the main body 50 and the measurement block 2 when the plurality of plug members 11 individually seal the plurality of recesses 10 of the measurement block 2. By doing so, the openings of the plurality of recesses 10 are individually sealed. The seal member 12 can be formed by using a known material conventionally used as a seal member in order to prevent gas leakage.

The pair of first guide members 51 and the pair of second guide members 52 guide the main body 50 so as to slide straight up and down when the main body 50 slides up and down with respect to the chamber main body 3. The pair of first guide members 51 and the pair of second guide members 52 both have a rectangular outer shape and exhibit a plate-like body having a predetermined thickness.

The width of the first guide portion 51 is substantially the same as the width of the guide groove 37 of the chamber body 3. The width of the second guide portion 52 is larger than the width of the first guide portion 51, and is substantially the same as the width of the guide hole 39 of the chamber body 3. The pair of first guide portions 51 slides up and down along the corresponding guide grooves 37, and the pair of second guide portions 52 slides up and down along the corresponding guide holes 39, whereby the main body The portion 50 slides straight up and down with respect to the chamber body 3.

A fixing means 8 for detachably fixing the inner lid 5 to the chamber lid 4 is provided between the inner lid 5 and the chamber lid 4. When the chamber lid 4 covers the upper opening of the chamber body 3, the fixing means 8 temporarily holds the inner lid 5 on the chamber lid 4, so that the fixing means 8 is inside the chamber body 3 as shown in FIG. A space S is formed between the housed measurement block 2 and the inner lid 5. Then, by removing the inner lid 5 from the chamber lid 4, the inner lid 5 can slide up and down with respect to the chamber body 3, and by sliding the inner lid 5 downward, as shown in FIG. The plurality of recesses 10 of the measurement block 2 can be individually sealed by the plurality of plug members 11.

As shown in FIGS. 1 and 4, for example, the fixing means 8 faces at least one magnet 80 provided on one of the inner lid 5 and the chamber lid 4 and the magnet 80 on the other of the inner lid 5 and the chamber lid 4. It can be configured with at least one metal plate 81 provided so as to. In the present embodiment, the magnets 80 are fitted into the through holes 53 at the boundary positions between the main body 50 of the inner lid 5 and the pair of first guide members 51, respectively, and the lengths of the left and right side edges of the chamber lid 4 A metal plate 81 is attached to the central portion in the direction so as to protrude from the lower surface of the chamber lid 4. By the contact between the magnet 80 and the metal plate 81, the inner lid 5 is removably fixed to the lower surface of the chamber lid 4 as shown in FIG.

Next, the concentration measuring device 7 is not particularly limited as long as it can measure the concentration of a predetermined gas (for example, oxygen) in each recess 10 of the measuring block 2, and has various configurations. Can be used. In the present embodiment, the concentration measuring device 7 is an oxygen concentration measuring device, and as shown in FIGS. 1, 6 and 8, the concentration measuring device 7 is individually set in the bottoms of a plurality of recesses 10 of the measuring block 2 and of the excitation light. It is composed of a plurality of fluorescent oxygen sensors 70 that emit fluorescence with an intensity corresponding to the oxygen concentration by irradiation, and a known microplate reader 71.

The fluorescent oxygen sensor 70 emits intense fluorescence corresponding to the oxygen concentration in the recess 10 of the measurement block 2 which is the sample measurement chamber when irradiated with the excitation light. Specifically, it is formed by fixing an oxygen-sensitive fluorescent substance inside.

The microplate reader 71 includes a plurality of light emitting units (not shown) that individually irradiate excitation light to each fluorescent oxygen sensor 70 set in a plurality of recesses 10 of the measurement block 2, and a plurality of fluorescent oxygen. It has a structure including a plurality of light receiving units (not shown) for individually measuring the fluorescence emitted from the sensor 70. The light emitting unit is used to excite a fluorescent substance fixed to the fluorescent oxygen sensor 70 to emit fluorescence, and for example, a mercury lamp, a laser, an LED, or the like can be used. The light receiving unit receives fluorescence of an intensity corresponding to the oxygen concentration from the fluorescent oxygen sensor 70 excited by the light from the light emitting unit, and for example, a cooled CCD or the like can be used. A control device (not shown) is connected to the microplate reader 71. The control device receives the fluorescence image obtained by the microplate reader 71, compares the fluorescence intensity information with the oxygen concentration calibration straight line, calculates the oxygen concentration profile and the oxygen consumption rate, and outputs the result. As the control device, a computer equipped with a CPU, ROM, RAM and I / O can be used.

In the present embodiment, the microplate reader 71 in which the light emitting unit and the light receiving unit are provided in the same device is used, but the excitation light source as the light emitting unit and the fluorescence intensity acquisition device as the light receiving unit are separate. It may be the device of.

The fluorescent oxygen sensor 70 is set at the bottom of each recess 10 of the measurement block 2, while the microplate reader 71 is set below the bottom 30 of the chamber body 3. A plate-shaped sensor fixing member 9 having a predetermined thickness is fixed to the lower surface of the bottom 30 of the chamber body 3 by using screws 91 (FIG. 1) or the like. The sensor fixing member 9 may be made of metal, glass, or synthetic resin, but is preferably made of transparent synthetic resin such as acrylic.

As shown in FIGS. 1, 6 and 8, the sensor fixing member 9 has a substantially rectangular outer shape, and an opening 90 for positioning around the microplate reader 71 is formed in the central portion thereof. By arranging the microplate reader 71 in the opening 90, the plurality of recesses 10 of the measurement block 2 can be individually irradiated with the excitation light. The bottom 30 of the chamber body 3 forms a space below which the microplate reader 71 is installed due to the height of the screw 91.

Next, the procedure of a method for measuring the photosynthetic activity of a plant, for example, using the measuring device 1 having the above-described configuration will be described. First, as shown in FIG. 5, the fluorescent oxygen sensor 70 is set in the bottom of each recess 10 of the measurement block 2 with the measurement block 2 housed in the chamber body 3.

Then, a part of the leaf is set as the measurement sample 100 with the light-shielding sheet 73 and / or the breathable cushioning material 72 placed on the fluorescent oxygen sensor 70 in each recess 10 of the measurement block 2. The light-shielding sheet 73 prevents the light from the light irradiator 101, which irradiates all the recesses 10 from above the measurement block 2 described later, into the microplate reader 71 located below the measurement block 2. It is for doing. As the light-shielding sheet 73, for example, black felt or the like can be used, but the light-shielding sheet 73 is not particularly limited to this, and various materials having the same function can be used. Further, the cushioning material 72 is for preventing the measurement sample 100 from directly touching the fluorescent oxygen sensor 70, and for example, plastic wool or mesh can be used, but the cushioning material 72 is not particularly limited to this. However, various ones can be used as long as they have the same function.

Next, as shown in FIGS. 5 and 6, the chamber lid 4 is covered with the upper opening of the chamber body 3 in a state where the inner lid 5 is fixed to the lower surface of the chamber lid 4. As a result, the space S in the chamber body 3 is closed. In this state, by introducing the CO _2- containing gas into the chamber main body 3 from the gas introduction port 6A, all the recesses 10 of the measurement block 2 are simultaneously replaced by the CO _2- containing gas.

Then, as shown in FIGS. 7 and 8, the inner lid 5 and the chamber lid 4 are unfixed, and the inner lid 5 is slid downward with respect to the chamber body 3 to measure with the plurality of plug members 11. The openings of the plurality of recesses 10 of the block 2 are closed. As a result, all the recesses 10 of the measurement block 2 are individually sealed at the same time, and all the recesses 10 are in a state of being filled with the CO ₂ containing gas.

Next, the chamber lid 4 is removed from the chamber body 3, and as shown in FIG. 9, the inner lid 5 is fixed to the measurement block 2 using screws 13 or the like, so that all the recesses 10 of the measurement block 2 are formed. It is tightly sealed.

Then, as shown in FIG. 10, all of the measurement block 2 is subjected to the light irradiator 101 from above the measurement block 2 while flowing water at a predetermined temperature through the flow path 24 so that the temperature becomes the optimum temperature for photosynthesis of plants. The recess 10 is irradiated with light.

As a result, the measurement sample 100 in each recess 10 of the measurement block 2 receives light to perform photosynthesis, carbohydrates are synthesized from carbon dioxide and water in the recess 10, and oxygen is released into the recess 10. On the other hand, the fluorescent oxygen sensor 70 in the recess 10 emits fluorescence by being irradiated with excitation light from the microplate reader 71, and this fluorescence is measured by the microplate reader 71. When oxygen is released from the measurement sample 100 into the recess 10 by photosynthesis, the fluorescence emitted from the fluorescent oxygen sensor 70 weakens according to the oxygen concentration in the recess 10. Therefore, by measuring the intensity of fluorescence emitted from the fluorescent oxygen sensor 70 with time by the microplate reader 71, the change in oxygen concentration in the recess 10 can be measured, and the photosynthetic activity of the measurement sample 100 is measured. be able to.

As described above, in order to measure the photosynthetic activity of a plant, it is necessary to replace the inside of a plurality of recesses 10 of the measurement block 2 serving as a sample measurement chamber with a CO _2- containing gas, but the measuring apparatus of the present embodiment According to No. 1, all the recesses 10 of the measurement block 2 can be replaced with the CO _2- containing gas all at once, and all the recesses 10 can be individually sealed at the same time. Therefore, since the photosynthetic activity of a plurality of measurement samples can be measured at the same time, it is possible to efficiently measure a large number of measurement samples, and the measurement time can be significantly shortened.

Further, a flow path 24 through which the liquid passes is formed inside the measurement block 2, and the temperature inside each recess 10 of the measurement block 2 can be adjusted to a desired temperature by adjusting the temperature of the liquid flowing through the flow path 24. Can be maintained at. Therefore, by setting the temperature in each recess 10 to a desired temperature according to the activity to be measured, the activity such as photosynthetic activity can be satisfactorily measured.

Further, the inner lid 5 is detachably fixed to the chamber lid 4 that closes the upper opening of the chamber body 3, and the inner lid 5 is positioned above the measurement block 2 shown in FIG. 6 at intervals. , The state can be easily switched to the state located directly above the measurement block 2 shown in FIG. Therefore, when introducing an arbitrary gas such as a CO _2- containing gas into the chamber body 3, it is placed at the position shown in FIG. 6 and the inside of each recess 10 of the measurement block 2 is an arbitrary gas such as a CO _2- containing gas. After the replacement with gas, the opening of the recess 10 of the measuring block 2 can be easily closed by the plug member 11 of the inner lid 5 simply by removing the fixing between the inner lid 5 and the chamber lid 4.

Although one embodiment of the present invention has been described above, the present invention is not limited to the above embodiment, and various modifications can be made without departing from the spirit of the present invention.

For example, in the above embodiment, the fixing means 8 is composed of the magnet 80 and the metal plate 81, but the inner lid 5 may be temporarily fixed to the chamber lid 4 and further removed from the chamber lid 4. For example, various other configurations can be used.

Further, in the above embodiment, when measuring the photosynthetic activity, the change with time of the oxygen concentration in each recess 10 of the measurement block 2 is measured, but the change with time of the carbon dioxide concentration may be measured. .. Further, although the measurement target is a plant, the measurement target is not limited to plants, for example, aquatic organisms such as algae, these cells (cells isolated from plants and algae or cultured cells), and further organelles. It may be an organ (for example, chloroplast or mitochondria). When the photosynthetic activity of algae, cells, etc. is used as a measurement sample, the change in pH of the liquid (water, cell preservation solution, cell culture solution, etc.) contained in each recess 10 together with the measurement sample over time is measured, for example, fluorescence. Photosynthetic activity may be measured by measuring using a pH measuring device such as a formula pH sensor.

Further, in the above embodiment, the measuring device 1 is used to measure the photosynthetic activity of the measurement sample, but the organism, its cells (cells isolated from the organism or cultured cells) and organelles (for example, chloroplast) are used. The measuring device 1 can also be used to measure the respiratory activity of (body and mitochondria). In this case, an arbitrary oxygen-containing gas is replaced in each recess 10 of the measurement block 2, and is housed in each recess 10 together with the concentration of oxygen consumed by respiration, the concentration of carbon dioxide generated by respiration, or the measurement sample. Respiratory activity can be measured by measuring changes over time such as pH of a liquid (water, cell preservation solution, cell culture solution, etc.) using a measuring device. When measuring respiratory activity, the cushioning material 72 and the light-shielding sheet 73 are not always necessary.

Furthermore, in addition to photosynthetic activity and respiratory activity, other activities that can be measured based on information on oxygen concentration or carbon dioxide concentration can also be measured using the measuring device 1. Moreover, other activities that can be measured based on the information on the oxygen concentration and the concentration of a predetermined gas other than carbon dioxide can also be measured by using the measuring device 1. In this case, the inside of each recess 10 of the measurement block 2 may be replaced with an arbitrary gas according to the activity to be measured, and information on the concentration of a predetermined gas may be measured using a measuring instrument suitable for the measurement target.

Further, in the above embodiment, the measuring instrument 7 for measuring the gas concentration such as the oxygen concentration in each recess 10 of the measuring block 2 measures an index depending on the gas concentration (for example, the fluorescence intensity of the fluorescent gas sensor). The gas concentration is measured by the above, but if the gas concentration can be measured, the gas concentration may be directly measured. Further, the gas concentration may be measured as an absolute value, a relative value, or a time change or the like.

Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited thereto.

Test example
1. 1. Test procedure The photosynthetic activity was measured using the measuring device of the above-described embodiment. A part of the leaf (tomato leaf, circle with a diameter of 8 mm (made by cutting one leaf into a circle with a diameter of 8 mm)) was used as a measurement sample.

More specifically, with the measurement block 2 housed in the chamber body 3 shown in FIG. 5, the fluorescent oxygen sensor 70 is located at the bottom of four recesses 10 among the plurality of recesses 10 of the measurement block 2. (Product name Oxygen Sensor Spot, manufactured by PreSens) was set. Next, a light-shielding sheet 73 (black felt) is placed on the fluorescent oxygen sensor 70 in the four recesses 10, and then a part of the above-mentioned leaf (leaf disc) is used as the measurement sample 100, and the back surface of the leaf is on the lower side. I put it horizontally so that it became. Next, as shown in FIGS. 5 and 6, with the inner lid 5 fixed to the lower surface of the chamber lid 4, the chamber lid 4 is covered with the upper opening of the chamber body 3 to form a space S in the chamber body 3. It was closed.

In this state, CO ₂ containing gas (composition: 5% CO ₂ , 5% O ₂ , 90% N ₂ ) is introduced into the chamber body 3 from the gas inlet 6A, and inside all the recesses 10 of the measurement block 2. Was replaced all at once with a CO _2- containing gas. Next, as shown in FIGS. 7 and 8, the inner lid 5 and the chamber lid 4 are unfixed, the inner lid 5 is slid downward with respect to the chamber body 3, and the plurality of plug members 11 are used for measurement. The openings of the plurality of recesses 10 of the block 2 were closed, and all the recesses 10 of the measurement block 2 were simultaneously and individually sealed, and all the recesses 10 were filled with CO ₂ containing gas. Next, the chamber lid 4 was removed from the chamber body 3, and the inner lid 5 was fixed to the measurement block 2 using screws 13 as shown in FIG. In this state, water at 23 ° C. was flowed through the flow path 24 to circulate.

Finally, a blackout curtain was placed on the measuring device 1 and the measurement of the oxygen concentration was started. When the change in oxygen concentration in the dark place became constant, the LED light provided on the measuring device 1 was turned on, and the measurement sample was irradiated with light (On in FIG. 11). A photosynthesis inhibitor (DUMU (3- (3,4-dichlorophenyl) -1,1-dimethylurea)) was allowed to act on two of the four measurement samples. The result is shown in FIG.

In addition, a fluorescent oxygen sensor and a measurement sample (leaf disk) are set in the four recesses 10 in the same manner as described above except that the leaves of ginkgo are used as the measurement sample and the photosynthesis inhibitor is not acted on all of the measurement samples. The measurement of oxygen concentration was started. In this case, the LED light was turned off 5 minutes after the LED light was turned on (Off in FIG. 12). The result is shown in FIG.

2. 2. Results In FIG. 11, in the measurement samples (DUMU (1), DUMU (2)) on which the photosynthesis inhibitor was acted, no significant increase in oxygen concentration was observed even under light irradiation, but the photosynthesis inhibitor was used. In the non-acting measurement samples (Non (1), Non (2)), a significant increase in oxygen concentration was observed under light irradiation. As a result, it was found that the measuring device can simultaneously and temporally measure the photosynthetic activity of a plurality of measurement samples based on the oxygen concentration.

Further, in FIG. 12, in all the measurement samples, an increase in oxygen concentration was observed under light irradiation, and it was confirmed that the increase in oxygen concentration stopped as the light irradiation was stopped. As a result, it was found that the measuring device can simultaneously and temporally measure the photosynthetic activity of a plurality of measurement samples based on the oxygen concentration.

From these facts, it was found that according to the measuring apparatus of the present invention, the photosynthetic activity of a plurality of measurement samples can be measured simultaneously and over time based on the oxygen concentration. Further, as described above, according to the measuring device of the present invention, the oxygen concentration can be measured simultaneously and with time for a plurality of measuring samples, so that the measuring device includes respiratory activity regardless of photosynthetic activity. It was found that it is useful for measuring the activity that can use the change in oxygen concentration as an index. In addition, it was found that the measuring device of the present invention is useful for measuring the activity that can use not only oxygen but also other changes in gas concentration, pH, etc. as indicators by using different fluorescent sensors. Since the measuring device of the present invention can measure the activities of a plurality of measurement samples simultaneously and over time, it can measure efficiently in a short time as compared with the conventional method in which only one sample can be measured at one time. I understood.

1 Measuring device 2 Measuring block 3 Chamber body 4 Chamber lid 5 Inner lid 6A Gas inlet 6B Gas outlet 7 Concentration measuring instrument 8 Fixing means 10 Recess 11 Plug member 12 Sealing member 21 1st plate 22 2nd plate 24 Flow path 25 Water supply port 26 Drain port 23 Packing 70 Fluorescent oxygen sensor 71 Micro plate reader (light emitting part and light receiving part)
72 Cushioning material 73 Light-shielding sheet 80 Magnet 81 Metal plate 100 Measurement sample 101 Light irradiator

Claims (9)

A measuring device for simultaneously measuring information on the concentration of a predetermined gas in a sample measuring chamber.
A measurement block with multiple recesses that serve as a sample measurement chamber,
The chamber body that houses the measurement block and
A chamber lid that closes the upper opening of the chamber body and
An inner lid that can slide up and down with respect to the chamber body and has a plurality of plug members on the lower surface that individually seal a plurality of recesses of the measurement block as the measuring block moves downward.
A gas inlet that is attached to the chamber body and introduces an arbitrary gas into the space between the measurement block and the inner lid in the chamber body, and a gas outlet that derives an arbitrary gas from the space.
A measuring device including a measuring device capable of measuring information on the concentration of the predetermined gas in each recess of the measuring block. The measuring device according to claim 1, further comprising a fixing means for detachably fixing the inner lid to the chamber lid. The fixing means comprises at least one magnet provided on one of the inner lid and the chamber lid, and at least one metal plate provided on the other of the inner lid and the chamber lid so as to face the magnet. The measuring device according to claim 2. The inner lid further includes a plurality of individually provided sealing members around the plurality of plug members.
The plurality of sealing materials are sandwiched between the inner lid and the measurement block when the plurality of plug members individually seal the plurality of recesses of the measurement block to open the openings of the plurality of recesses. The measuring device according to any one of claims 1 to 3, which is individually sealed. The measurement block is
A first metal plate with multiple through holes and
A second plate fixed to the first plate so as to close an opening on one side of a plurality of through holes of the first plate to form a plurality of recesses is provided.
The second plate is capable of transmitting light and is capable of transmitting light.
The first plate is internally formed with a flow path through which the liquid passes so as to collectively surround the plurality of through holes, and a water supply port for supplying the liquid to the flow path and a flow path for discharging the liquid. The measuring device according to any one of claims 1 to 4, wherein a drainage port is provided. The measuring device according to any one of claims 1 to 5, wherein a packing for sealing an opening on one side of the plurality of through holes is sandwiched between the first plate and the second plate. The measuring instrument is an oxygen concentration measuring instrument for measuring an oxygen concentration.
The oxygen concentration measuring device is individually set on the bottoms of a plurality of recesses of the measuring block, and a plurality of fluorescent oxygen sensors that emit fluorescence of intensity according to the oxygen concentration by irradiation with excitation light, and a plurality of fluorescent oxygen sensors.
Claims 1 to 1, further comprising a plurality of light emitting units for individually irradiating the plurality of fluorescent oxygen sensors with excitation light, and a plurality of light receiving units for individually measuring fluorescence emitted from the plurality of fluorescent oxygen sensors. 6. The measuring device according to any one of 6. The measuring device according to claim 7, wherein a sample is housed on the fluorescent oxygen sensor in each of the plurality of recesses of the measuring block via a light-shielding sheet and / or a breathable cushioning material. The measuring device according to any one of claims 1 to 8, further comprising a light irradiator that irradiates all the recesses of the measuring block with light.