JP2020115837A - Cell culture management system - Google Patents

Abstract

To provide a cell culture management system.SOLUTION: A cell culture management system of the present invention comprises: an output data server provided for a first culture apparatus for culturing a first cell; a customer server provided for a second culture apparatus for culturing a second cell; a cell management server provided for a cell management repository storing the second cell, in which the output data server, the customer server, and the cell management server are severally connected to a data management device such that the servers are capable of sending/receiving data to/from the device.SELECTED DRAWING: Figure 1

Description

The present invention relates to a cell culture management system.

Conventionally, various types of culture devices and culture methods have been developed for the purpose of maintaining organ function and growth for a long period of time. For example, Patent Document 1 discloses a growth induction system, a growth induction control device, a growth induction control method, and a growth induction control program, which are capable of inducing growth without requiring addition of a cytokine at each growth stage. ing.

International publication 2017/191691 pamphlet

However, when such a cell culture system is individually arranged, there has never been a system that can be managed as a whole.

Therefore, an object of the present invention is to provide a novel cell culture management system.

According to one embodiment of the present invention, a data management device, an output data server provided in a first culture device for culturing a first cell, and a second culture device for culturing a second cell. A cell culture management system comprising a customer server provided in a cell management server, and a cell management server provided in a cell management repository storing second cells, comprising a data management device, an output data server, and a customer. The server and the cell management server are connected to each other so as to be able to transmit and receive, and the output data server has a transmission means for transmitting a first protocol for culturing the first cell to the data management device. Then, the customer server has one or more transmission means for transmitting the order information for obtaining the second cells and the position information of the second culture device to the data management device, and A second protocol for culturing the second cell from the data management device, the data management device for receiving the first protocol from the output data server. Receiving means, the receiving means for receiving the order information and the position information of the second incubator from the customer server, the first protocol received from the output data server, and the customer server The cell management server has one or more storage means for storing the second protocol, the order information, and the position information of the second incubator received from the cell management server. And a reception means for receiving the position information of the second bioreactor from the data management device. The output data server may include receiving means for receiving a third protocol for culturing the third cell from the data management device. The customer server has a transmission unit for transmitting the actual culture data obtained from the cell sensor installed in the second culture device to the data management device, and the data management device is transmitted from the customer server. It may have a receiving means for receiving the actually measured culture data, and a storage means for storing the actually measured culture data received from the customer server. The cell management server includes storage means for storing inventory information of cells existing in the cell management storage, and transmission means for transmitting the inventory information to the data management device. The device may include a receiving unit for receiving the inventory information transmitted from the cell management server, and a storage unit for storing the received inventory information.

Another embodiment of the present invention comprises an output data server provided in the first culture device for culturing the first cell and a second culture device for culturing the second cell. A customer server and a cell management server provided in a cell management repository storing the second cell,
A cell culture management system comprising: the output data server, the customer server, and the cell management server, each of which is connected to a data management device so as to be capable of transmitting and receiving. A transmission means for transmitting a first protocol for culturing cells to the data management device, wherein the customer server has order information for obtaining the second cells and position information of the second culture device. And one or more transmitting means for transmitting to the data management device, and a receiving means for receiving a second protocol for culturing the second cell from the data management device. The cell management server is a cell culture management system having the order information and the position information of the second culture device, and a receiving unit for receiving the position information of the second culture device from the data management device. The output data server may include receiving means for receiving a third protocol for culturing the third cell from the data management device. The customer server has a transmission unit for transmitting the actual culture data obtained from the cell sensor installed in the second culture device to the data management device, and the data management device is transmitted from the customer server. It may have a receiving means for receiving the actually measured culture data, and a storage means for storing the actually measured culture data received from the customer server. The cell management server includes storage means for storing inventory information of cells existing in the cell management storage, and transmission means for transmitting the inventory information to the data management device. The device may include a receiving unit for receiving the inventory information transmitted from the cell management server, and a storage unit for storing the received inventory information.

The present invention has made it possible to provide a novel cell culture management system.

It is a flowchart which shows operation|movement of the cell culture system in the basic process which is one Embodiment concerning this invention. It is a schematic diagram of the cell culture system in the basic process which is one Embodiment concerning this invention. The broken line means the flow of information, and the solid line means the flow of things. It is a schematic diagram of the cell culture system at the time of development of the novel protocol which is other embodiment concerning this invention. The broken line means the flow of information, and the solid line means the flow of things. It is a schematic diagram of the cell culture system at the time of feedback of the culture conditions which is other embodiment concerning this invention. The broken line means the flow of information, and the solid line means the flow of things. It is a schematic diagram of the cell culture system in inventory management which is other embodiment concerning this invention. Lines mean the flow of information, and solid lines mean the flow of things.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings, but are not necessarily limited thereto. The objects, features, advantages, and ideas of the present invention will be apparent to those skilled in the art from the description of the present specification, and those skilled in the art can easily reproduce the present invention from the description of the present specification. it can. The embodiments and specific examples of the invention described below show preferred embodiments of the present invention, and are shown for the purpose of illustration or explanation, and the present invention is not limited thereto. It is not limited. It will be apparent to those skilled in the art that various alterations and modifications can be made based on the description of the present specification within the intent and scope of the present invention disclosed in the present specification.

==Cell culture management system==
A cell culture management system according to an embodiment of the present invention includes an output data server provided in a first culture device for culturing a first cell, and a second culture device for culturing a second cell. A cell culture management system comprising: a customer server provided in a cell management system; and a cell management server provided in a cell management repository storing second cells, the output data server, the customer server, and the cell management system. The server is connected to a data management device outside the system so as to be able to transmit and receive, respectively, and the output data server has a transmission means for transmitting the first protocol for culturing the first cell to the data management device. The customer server has one or more transmitting means for transmitting the order information for obtaining the second cells and the position information of the second culture device to the data management device, and the second. Receiving means for receiving a second protocol for culturing cells of the above from the data management device, and the cell management server stores the order information and the position information of the second bioreactor as data. Receiving means for receiving from the management device. Here, the data management device includes a receiving means for receiving the first protocol from the output data server, a receiving means for receiving the order information and the position information of the second incubator from the customer server. , One or more storage means for storing the first protocol received from the output data server, the second protocol received from the customer server, the order information, and the location information of the second bioreactor. Have. Hereinafter, a specific embodiment of this system will be described with reference to FIG.

<Data management device>
The data management device 1 includes one or a plurality of output data servers 3, one or a plurality of customer servers 7, and 1 via a network such as the Internet, a LAN (Local Area Network), a WAN (Wide Area Network), and a dedicated line. Alternatively, it is connected to a plurality of cell management servers 11 so as to be able to transmit and receive. The data management device 1 includes at least a receiving means for receiving the protocol from the output data server 3, a receiving means for receiving the order information from the customer server 7, and the position information of the culture device 8. It has a storage means 2 for storing the received data. The received data is preferably stored as a database.

The data management device 1 may be a device outside the cell culture management system like a cloud, or may be managed as a part of the cell culture management system.

Further, the data management device 1 may be a device which is simply composed of a transmission/reception means and a storage means 2 and only holds data, but has a data processing means other than the transmission/reception means and the storage means 2 to store data Various processes may be performed.

<Output data server>
The output data server 3 is provided in the culture device 4 for developing a new protocol for culturing cells. Then, it has a transmission means for transmitting a new protocol to the data management device 1. The protocol includes, for example, cell types for cell culture, culture conditions (for example, temperature and CO ₂ concentration), type of medium used, reference values of cell markers to be measured over time, and medium to be measured over time. Information such as pH reference value and reference value of cell density measured over time may be included.

The culture device 4 is not particularly limited, and any known culture device can be used. For example, the culturing device 4 may include only a incubator for culturing cells, but may include a bioreactor 6. Here, the bioreactor 6 is, for example, one or more culture tank tanks for culturing cells to obtain a cell product, a control unit for controlling cell culture, and a culture including culture conditions. A storage unit for storing the protocol is included. When using a plurality of culture tanks, the culture solution is refluxed in the culture tanks, and thus each culture tank may be provided with a separate culture solution reflux mechanism, or the plurality of culture tanks can circulate the culture solution to each other. When connected to the medium, a culture medium circulator may be provided. The control unit for controlling the culture may control the medium flow rate and the reflux direction via the culture solution reflux mechanism.

Further, it is preferable that the culturing device 4 has a cell sensor 5 that can automatically and temporally detect the state of cell culture. The cell sensor 5 is a physical, chemical or biological property such as weight, temperature, viscosity, color tone (absorbance), pH, sugar content, salt concentration, density and type of cells, medium or cell product in a culture tank. Is preferably obtained over time. The cell sensor 5 may be controlled by the controller. The conditions detected by the cell sensor 5 may be stored in the storage unit.

Further, the data management device 1 may have a transmission means for transmitting the culture protocol to the output data server 3, while the output data server 3 may have a reception means for receiving the culture protocol from the data management device 1. Although a culture protocol creator may create an entirely new protocol, having the means to receive the protocol allows the modification of an existing culture protocol to create a new protocol.

<Customer server>
The customer server 7 is provided in a culture device 8 for a customer to culture cells. The configuration of the culture device 8 may be the same as that of the culture device 4 attached to the output data server 3, and may include, for example, the same bioreactor 10 and the same cell sensor 9. In particular, when a new protocol is developed in the culture device 4 attached to the output data server 3, it is preferable that the same device is used in order to meet the conditions.

For the customer, the purpose of the culture is not particularly limited, but for example, cells for food, medical use, or materials may be grown, or the substance may be produced using the cells.

The customer server 7 includes a transmission means for transmitting the order information and the position information of the culture device 8 to the data management device 1. The order information is not limited as long as it describes the purpose of the customer. For example, if a cell is required, the name of the target cell itself may be used, and if a cell product is required, the name of the cell product may be used. Further, in addition to the name of the target cell and the target cell product, information for ordering a product may be usually included. For example, it is possible to calculate the time when the cells are delivered from the cell management by including the necessary time information of the cells and cell products. On the other hand, the position information of the culture device 8 means the place where the culture device 8 exists. The place can be specified by an address or the like.

Further, the customer server 7 has a receiving means for receiving a protocol for obtaining a target cell or cell product from the data management device 1. The protocol received from the data management device 1 may be stored in the storage unit provided in the bioreactor.

Further, the customer server 7 is a protocol standard that is obtained over time from a cell sensor installed in the bioreactor while culturing cells in the culturing device 8 or obtained from the cell sensor at the end of culturing. You may have the transmission means for transmitting the measured culture data containing the measured value corresponding to a value (for example, pH value or the reference value of cell density) to the data management apparatus 1. By having such a means, it becomes possible to detect and control the variation when manufacturing the same cell product in a plurality of bioreactors. In this case, the data management device may include a receiving unit for receiving the actual culture data and a storage unit for storing the data. This storage means may be the same as or different from the storage means for storing other data.

When the bioreactor 10 uses a plurality of culture tanks, the initial arrangement information of the cultured cells (for example, the culture tank number in the bioreactor) and instructions for changing the recirculation condition of the medium between the culture tanks over time are also included. It may be included in the protocol.

<Cell management server>
The cell management server 11 is provided in the cell management storage 12 that stores cells. The data management device 1 sends order information from the customer sent by the customer server and data for culturing target cells to the customer. It has a receiving means for receiving the position information of the culture device 8 and the information. Furthermore, the culture protocol and the conveyed product information that accompany the order information may be received from the data management device 1. The conveyed product information is information indicating a conveyed product to be conveyed to the customer in response to an order from the customer, and includes information about materials necessary for culture (for example, consumables such as culture medium). Based on these pieces of information, it becomes possible to carry the goods required by the customer to the place where the culture device 8 is located.

The cell management server 11 may have a transmission unit for transmitting the inventory information of various cells stored in the cell management storage or in the cell management server 11 to the data management device 1. A cell administrator who has accessed the data management device 1 can replenish cells with little or no inventory by purchasing the cells or growing the cells themselves.

It should be noted that the cell management warehouse may include a warehouse, a refrigerator, a freezer, a culture tank, etc. for storing the cells and carrying them out when instructed.

==Cell culture management method==
How the cells can be managed using the above cell culture management system will be described in detail with reference to some embodiments.

(1) Basic process (Figs. 1-2)
The basic steps in the cell culture management method using this management system will be described with reference to FIGS.

The culturing protocol creator sends a culturing protocol in which the names and IDs of cells and/or cell products are associated with the cell culturing method related to them, to the data management device 1 via the output data server 3 (S1). The data management device 1 receives the data transmitted from the output data server 3 and stores it in the storage means 2 (S2) (A). The cell culture method is a method developed by the creator of the culture protocol. At that time, the culture conditions in the bioreactor 6 detected by the cell sensor 5 may be included in the culture protocol.

The customer places an order with the data management device 1 via the customer server 7 to obtain the target cells. At that time, when the position information of the culture device 8 for producing cells and the like are transmitted to the data management device 1 together with the order information (S3), the data management device 1 receives the data transmitted from the customer server 7, and the storage means. 2 (S4)(B). The order information may be information about cells that the customer wants to culture or information about cell products that the customer wants to produce using the cultured cells. The information in this case is preferably the name of the cell or cell product, but the data management device manages the cultured cell, cell product, culture protocol, etc. by ID, and the customer can know the ID. In this case, the information may be the ID.

When the data management device 1 receives an order from the customer server 7 and transmits a culture protocol for culturing cells (S5), the customer receives the data transmitted from the data management device 1 through the customer server 7. (S6) (C). This is because the data management device 1 may automatically transmit the culture protocol corresponding to the order information in response to the reception of the order information, and the customer can select an appropriate culture protocol from the data in the data management device 1. You may select and download.

On the other hand, the data management device 1 that has received the order from the customer transmits the order information from the customer, the position information of the culture device of the customer, and the like (S7), and the cell management server 11 provided in the cell management cabinet 12 Receives data from the data management device 1 (S8)(D). The data reception by the cell management server 11 may be performed by the data management device 1 at regular intervals, for example. However, when the manager of the cell management cabinet 12 is convenient, the data management device 1 is appropriately used. You may go to get the data. Alternatively, the data management device 1 that has received the order may automatically transmit it to the cell management server 11 together with the position information of the customer's culture device. Then, the manager of the cell management cabinet 12 carries the goods required by the customer to the culture device 8 of the customer based on the received data.

The customer installs the cells in the culture tank of the bioreactor 10 according to the received culture protocol using the delivered product received from the cell management box 12, starts the culture, and grows the target cells. Alternatively, the cell product resulting from cell growth may be recovered.

(2) Creation of new protocol (Fig. 3)
An embodiment in which the output data server 3 sends a new protocol to the data management device 1 will be described with reference to FIG.

The culturing protocol creator can develop a completely new protocol and send it to the data management device 1 with a new control number (A). Alternatively, the existing protocol a is received from the data management device 1 using the output data server 3 (E), and is studied using the culture device 4, a new protocol b is developed from the protocol a, and a new protocol b is added. It may be transmitted to the data management device 1 together with the management number (A).

(3) Feedback from customers (Fig. 4)
An embodiment in which the customer server 7 feeds back the measured culture data to the data management device 1 will be described with reference to FIG.

The customer accesses the data management device 1 via the customer server 7 and places an order. At that time, the order information and the position information of the culture device 8 for culturing the target cells are transmitted to the data management device 1 (B). The data management device 1 receives the information and stores it in the storage means 2. Accordingly, the customer server 7 receives the culture protocol for culturing the target cells from the data management device 1 (C).

When the cell management server 11 provided in the cell management cabinet 12 receives the order information from the customer and the position information of the culture apparatus of the customer from the data management apparatus 1, the administrator of the cell management cabinet 12 receives the information. Based on the data obtained, the cells are transported to the customer's culture device 8 (D).

The customer may load the received protocol into the bioreactor 10 and start culturing the cells in a predetermined fermentor tank of the bioreactor 10. At that time, the customer server 7 is obtained from the cell sensor 9 installed in the culture device 8 over time while culturing the target cell, or obtained from the cell sensor at the end of culturing the target cell. Or, it is possible to transmit the actual culture data including the actual measurement value corresponding to the reference value of the protocol (for example, the reference value of the pH value or the cell density) to the data management device 1 (F). The data management device 1 may receive the actual culture data and store it in the storage means 2.

By managing such measured culture data with the data management device 1, the culture protocol creator can freely use the measured culture data when developing the protocol.

(4) Inventory management (Fig. 5)
An embodiment in which the cell management server 11 manages the cell inventory will be described with reference to FIG.

The cell management server 11 may transmit the cell inventory information to the data management device 1 (G). The cell inventory information may be updated periodically, but the inventory information may be updated only when the cell inventory information changes in the cell management server 11. The data management device 1 manages the inventory information as a database in the storage device 2. However, when new inventory information is transmitted from the cell management server 11, the data management device 1 receives the inventory information and updates the database of the storage device 2. .. The inventory information may include not only the inventory amount but also expiration date information.

The administrator of the cell management cabinet 12 accesses the data management apparatus 1 to newly bring in cells to the cell management cabinet 12 with respect to cells with little or no stock, expired cells, or the like. Alternatively, the cells are grown by using the culture device 13 provided in the cell management cabinet 12 to secure the cell inventory. The culturing device 13 may be the same as or different from the culturing device 4 of the output data server 3 and the culturing device 4 of the customer server 7, but is preferably the same type, and includes the cell sensor 14 and the bioreactor 15. It is preferable.

== Specific example of operation using a cell culture management system ==
Here, the operation example of the cell culture management system from the customer's side is described. According to these embodiments, the customer can specify a target cell or cell product and obtain a culture protocol and cells suitable for producing the target cell or cell product in a predetermined culture device. Culturing cells using a culture protocol allows cells or cell products to be produced with good reproducibility.

(1) When Customer Orders Cells The customer is provided with a culture device for culturing target cells in advance.

The customer accesses the data management device 1 via the customer server 7 when it becomes necessary to culture the cells. The data management device 1 displays a list of orderable cells and a purchase order as a database. The customer selects a cell from the list and clicks on the cell name or ID or writes the cell name or ID, and at the same time, describes the address of the culture device 8 that manufactures the cell in the order form. Finally, these pieces of information are transmitted to the data management device 1.

Customers can download the culture protocol associated with the ordered cells. The culture protocol describes the method of culturing the target cells (for example, the type of medium and culture conditions).

The administrator of the cell management box 12 who has learned the order from the data management device 1 delivers the delivery item required for culturing the cell to the customer, who is associated with the cell, and the customer receives the delivery item. Then, the customer cultivates the target cells using the culturing device, using the received delivery product and according to the downloaded culturing protocol.

(2) When a customer orders a cell product The customer is provided with a culture device for culturing cells to obtain a desired cell product in advance.

The customer accesses the data management device 1 through the customer server 7 when it becomes necessary to obtain the cell product. The data management device 1 displays a list of cell products that can be produced and an order form as a database. The customer selects a cell product from the list, clicks on the cell product name or ID, writes in the cell product name or ID, and at the same time describes the address of the culture device 8 for producing the cells in the order form. .. Finally, these pieces of information are transmitted to the data management device 1.

The customer can download the culture protocol associated with the ordered cell product. The culture protocol describes a method for culturing cells to obtain a desired cell product (for example, cell type, medium type, culture conditions, etc.).

The administrator of the cell management warehouse 12 who has received the order from the data management device 1 is associated with the cell product, and delivers the goods required for cell culture for the production to the customer, and the customer carries the goods. Receive the goods. Then, the customer can obtain the target cell product by culturing the predetermined cells using the culturing device according to the downloaded culturing protocol by using the received delivery product.

1 Data management device 1
2 Storage Means 3 Output Data Server 4 Culture Device 5 Cell Sensor 6 Bioreactor 7 Customer Server 8 Culture Device 9 Cell Sensor 10 Bioreactor 11 Cell Management Server 12 Cell Management Cabinet 13 Culture Device 14 Cell Sensor 15 Bioreactor

Claims (8)

A data management device,
An output data server provided in the first culture device for culturing the first cell,
A customer server provided in the second culture device for culturing the second cell,
A cell management server provided in a cell management repository storing the second cell,
A cell culture management system comprising:
The data management device, the output data server, the customer server, and the cell management server are connected to each other so that they can be transmitted and received.
The output data server is
A transmission means for transmitting a first protocol for culturing a first cell to the data management device,
The customer server is
One or more transmitting means for transmitting the order information for obtaining the second cells and the position information of the second culture device to the data management device;
Receiving means for receiving a second protocol for culturing the second cells from the data management device,
The data management device,
Receiving means for receiving the first protocol from the output data server, receiving means for receiving the order information and position information of the second incubator from the customer server,
One or more of which stores the first protocol received from the output data server, the second protocol received from the customer server, the order information, and the position information of the second incubator. With the above storage means,
The cell management server is
A cell culture management system comprising: a receiving unit for receiving the order information and the position information of the second bioreactor from the data management device. The cell culture management system according to claim 1, wherein the output data server has a receiving unit for receiving a third protocol for culturing the third cell from the data management device. The customer server has a transmission unit for transmitting actually-measured culture data obtained from a cell sensor installed in the second culture device to the data management device,
The data management device,
Receiving means for receiving the measured culture data transmitted from the customer server,
Storage means for storing the measured culture data received from the customer server;
The cell culture management system according to claim 1 or 2, further comprising: The cell management server is
Storage means for storing inventory information of the cells existing in the cell storage,
Transmitting means for transmitting the inventory information to the data management device;
Have
The data management device,
Receiving means for receiving the inventory information transmitted from the cell management server,
Storage means for storing the received inventory information,
The cell culture management system according to claim 1, which comprises: An output data server provided in the first culture device for culturing the first cell,
A customer server provided in the second culture device for culturing the second cell,
A cell management server provided in a cell management repository storing the second cell,
A cell culture management system comprising:
The output data server, the customer server, and the cell management server are connected to a data management device so that they can be transmitted and received.
The output data server is
A transmission means for transmitting a first protocol for culturing a first cell to the data management device,
The customer server is
One or more transmission means for transmitting the order information for obtaining the second cells and the position information of the second culture device to the data management device;
Receiving means for receiving a second protocol for culturing the second cell from the data management device,
The cell management server is
A cell culture management system comprising: a reception unit for receiving the order information and the position information of the second culture device from the data management device. The cell culture management system according to claim 5, wherein the output data server has a receiving unit for receiving a third protocol for culturing the third cell from the data management device. The customer server has a transmission unit for transmitting measured culture data obtained from a cell sensor installed in the second culture device to the data management device,
The data management device,
Receiving means for receiving the actual culture data transmitted from the customer server,
Storage means for storing the measured culture data received from the customer server;
The cell culture management system according to claim 5, which comprises: The cell management server is
Storage means for storing inventory information of the cells existing in the cell storage,
Transmitting means for transmitting the inventory information to the data management device;
Have
The data management device,
Receiving means for receiving the inventory information transmitted from the cell management server,
Storage means for storing the received inventory information,
The cell culture management system according to any one of claims 5 to 7, which comprises:

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