JP2020059653A - Natural polymer compound composition, method for producing the same, food and medical material - Google Patents
Natural polymer compound composition, method for producing the same, food and medical material Download PDFInfo
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Abstract
Description
本発明は、天然高分子化合物組成物、その製造方法、食品および医療材料に関する。 The present invention relates to a natural polymer compound composition, a method for producing the same, a food product and a medical material.
近年、食の多様化が進み、鳥獣肉の内臓である腸や胃（以下、「ホルモン」という）が食材として非常に人気を博し、焼肉、鍋物、炒め物等様々な方法で食されている。ホルモンが食材として好まれる要因のひとつに食感がある。ホルモンは、粘性および弾性の両方を合わせた粘弾性と吸着性のある食感を有し、他の食品にない食感を楽しむことができる。 In recent years, dietary diversification has progressed, and the intestines and stomachs (hereinafter referred to as “hormones”), which are the internal organs of poultry meat, have become very popular as food ingredients, and are eaten in various ways such as grilled meat, pans, and stir-fried foods. There is. Texture is one of the factors that favor hormones as food ingredients. Hormones have a viscoelasticity that combines both viscosity and elasticity, and a texture with adsorptivity, and can enjoy a texture that is not found in other foods.
粘弾性の食感を得る技術として、ゼラチンにポリリジンを加え、トランスグルタミナーゼで架橋反応させて改質したゼラチンを作製し、その改質ゼラチンを使用してゼラチン加工品に耐熱性や吸水防止等の特殊な機能を付与する技術が知られている（例えば、特許文献１参照）。また、油脂、水溶性蛋白、水およびトランスグルタミナーゼを混合して架橋反応を行わせ、所定の固さと風味を有した固形脂を得る技術が知られている（例えば、特許文献２参照）。 As a technique for obtaining a viscoelastic texture, polylysine is added to gelatin and cross-linked by transglutaminase to produce modified gelatin, and the modified gelatin is used to improve heat resistance and water absorption of processed gelatin products. A technique of giving a special function is known (for example, refer to Patent Document 1). Further, a technique is known in which fats and oils, water-soluble proteins, water and transglutaminase are mixed to carry out a crosslinking reaction to obtain solid fats having a predetermined hardness and flavor (see, for example, Patent Document 2).
菓子類においては、糖質、ゼラチン及び油脂を所定の組成重量比率で含有し、ゼラチンをトランスグルタミナーゼで架橋処理して成る、ホルモン様のコリコリした食感のグミキャンディが知られている（例えば、特許文献３参照）。
食品以外の分野では、組織インプラントの製造技術であって、ゼラチンを水とグリセリンの混合物に溶解させ、その溶液にホルムアルデヒド水溶液を添加して架橋させ、混合物をホモジネートして乾燥させた後、さらにホルムアルデヒド水溶液で架橋させる技術が知られている（例えば、特許文献４参照）。
また、ゼラチン−多価アルコール溶液とトランスグルタミナーゼとを混合し、５０℃に暖めてゲル化させる技術が知られている（例えば、特許文献５参照）。
In confectionery, gummy candy containing sugar, gelatin and fats and oils in a predetermined composition weight ratio and formed by cross-linking gelatin with transglutaminase is known as a gummy candy with a hormone-like crunchy texture (for example, See Patent Document 3).
In fields other than food, a technique for producing tissue implants, in which gelatin is dissolved in a mixture of water and glycerin, an aqueous formaldehyde solution is added to the solution to crosslink, and the mixture is homogenized and dried, and then formaldehyde is added. A technique of crosslinking with an aqueous solution is known (see, for example, Patent Document 4).
Further, a technique is known in which a gelatin-polyhydric alcohol solution and transglutaminase are mixed and heated to 50 ° C to cause gelation (for example, refer to Patent Document 5).
しかしながら、特許文献１に記載の技術では、ポリリジンが用いられるという課題があった。特許文献２，３に記載の技術では、油脂が用いられるという課題があった。特許文献４，５に記載の技術では、粘弾性の性質を得ることができないという課題があった。 However, the technique described in Patent Document 1 has a problem that polylysine is used. The techniques described in Patent Documents 2 and 3 have a problem that fats and oils are used. The techniques described in Patent Documents 4 and 5 have a problem that viscoelastic properties cannot be obtained.
本発明は、このような課題に着目してなされたもので、ポリリジンや油脂などを使用せずに、粘弾性を有する天然高分子化合物組成物、その製造方法、食品および医療材料を提供することを目的としている。 The present invention has been made in view of such a problem, and provides a natural polymer compound composition having viscoelasticity, a method for producing the same, a food, and a medical material without using polylysine or fats and oils. It is an object.
前記目的を達成するため、本発明者らは鋭意検討を重ねた結果、ゼラチンまたはコラーゲンペプチドを多価アルコールまたはその誘導体の水溶液に溶解させ、トランスグルタミナーゼにより架橋反応させることにより、粘弾性がありながらも吸着性のある、従来ない食感の組成物が得られ、その食感は凍結乾燥等で水分を９９重量％以上除去しても保持されることを発見し、本発明を完成するに至った。 In order to achieve the above-mentioned object, the present inventors have conducted extensive studies, and as a result, gelatin or collagen peptide was dissolved in an aqueous solution of a polyhydric alcohol or a derivative thereof, and cross-linked by transglutaminase to give a viscoelasticity. It was found that a composition having a texture that is not adsorbed and has a conventional texture is obtained, and that the texture is retained even when 99% by weight or more of water is removed by freeze-drying or the like, leading to the completion of the present invention. It was
即ち、本発明に係る天然高分子化合物組成物は、多価アルコールまたはその誘導体を含む分子量３０，０００以上３００,０００以下の架橋した親水性天然高分子化合物を含むことを特徴とする。
本発明に係る天然高分子化合物組成物において、前記多価アルコールまたはその誘導体は重合度１〜１０のオリゴグリセリンまたはその誘導体から成ることが好ましい。
That is, the natural polymer compound composition according to the present invention is characterized by containing a crosslinked hydrophilic natural polymer compound containing a polyhydric alcohol or a derivative thereof and having a molecular weight of 30,000 or more and 300,000 or less.
In the natural polymer compound composition according to the present invention, it is preferable that the polyhydric alcohol or its derivative comprises oligoglycerin having a polymerization degree of 1 to 10 or its derivative.
本発明に係る天然高分子化合物組成物は、前記親水性天然高分子化合物の架橋剤を含むことが好ましい。
前記架橋剤はトランスグルタミナーゼを含むことが好ましい。
前記親水性天然高分子化合物は分子量３０，０００以上１００，０００以下のゼラチンまたはコラーゲンペプチドから成ることが好ましい。
The natural polymer compound composition according to the present invention preferably contains a crosslinking agent for the hydrophilic natural polymer compound.
The cross-linking agent preferably contains transglutaminase.
The hydrophilic natural polymer compound preferably comprises gelatin or collagen peptide having a molecular weight of 30,000 or more and 100,000 or less.
本発明に係る天然高分子化合物組成物は、全重量に対し、前記多価アルコールまたはその誘導体４８〜６８重量％、前記親水性天然高分子化合物２５〜４８重量％、８０から１３０Ｕ／ｇの前記架橋剤０．０２〜０．１３重量％を含むことが好ましい。
本発明に係る天然高分子化合物組成物は、多孔質体から成ることが好ましく、乾燥した多孔質体から成ることが特に好ましい。
The natural polymer compound composition according to the present invention comprises, based on the total weight, the polyhydric alcohol or a derivative thereof in an amount of 48 to 68% by weight, the hydrophilic natural polymer compound in an amount of 25 to 48% by weight, and 80 to 130 U / g. It is preferable to include 0.02 to 0.13% by weight of the crosslinking agent.
The natural polymer compound composition according to the present invention is preferably a porous body, and particularly preferably a dried porous body.
本発明に係る天然高分子化合物組成物の製造方法は、親水性天然高分子化合物を多価アルコールまたはその誘導体の水溶液に溶解させて親水性天然高分子化合物溶液を調製する溶液調製工程と、前記親水性天然高分子化合物溶液に架橋剤を混合して架橋反応させる架橋反応工程と、架橋反応させた前記親水性天然高分子化合物溶液を攪拌して泡を生じさせる起泡工程とを、有することを特徴とする。 The method for producing a natural polymer compound composition according to the present invention comprises a solution preparation step of preparing a hydrophilic natural polymer compound solution by dissolving a hydrophilic natural polymer compound in an aqueous solution of a polyhydric alcohol or a derivative thereof, A cross-linking reaction step of mixing a cross-linking agent in the hydrophilic natural polymer compound solution to carry out a cross-linking reaction, and a foaming step of stirring the cross-linking hydrophilic natural polymer compound solution to generate bubbles. Is characterized by.
前記架橋反応工程において、前記架橋剤はトランスグルタミナーゼを含み、前記親水性天然高分子化合物溶液に前記架橋剤を０℃以上４０℃以下で混合して架橋反応させることが好ましい。
前記親水性天然高分子化合物は分子量３０，０００以上３００，０００以下のゼラチンまたはコラーゲンペプチドから成り、前記多価アルコールまたはその誘導体は重合度１〜１０のオリゴグリセリンまたはその誘導体から成り、前記起泡工程において、前記親水性天然高分子化合物溶液をホモミキサーにより回転数５０００〜２００００ｒｐｍで５〜４０分間撹拌することが好ましい。
本発明に係る天然高分子化合物組成物の製造方法は、前記起泡工程後の前記親水性天然高分子化合物溶液を凍結乾燥させることが好ましい。
In the crosslinking reaction step, it is preferable that the crosslinking agent contains transglutaminase, and the hydrophilic natural polymer compound solution is mixed with the crosslinking agent at 0 ° C. or higher and 40 ° C. or lower to perform a crosslinking reaction.
The hydrophilic natural polymer compound is composed of gelatin or collagen peptide having a molecular weight of 30,000 or more and 300,000 or less, the polyhydric alcohol or its derivative is composed of oligoglycerin or its derivative having a polymerization degree of 1 to 10, and the foaming is performed. In the step, the hydrophilic natural polymer compound solution is preferably stirred with a homomixer at a rotation speed of 5000 to 20000 rpm for 5 to 40 minutes.
In the method for producing a natural polymer compound composition according to the present invention, it is preferable to freeze-dry the hydrophilic natural polymer compound solution after the foaming step.
本発明に係る食品は、前述の天然高分子化合物組成物を含むことを特徴とする。
本発明に係る医療材料は、前述の天然高分子化合物組成物を含むことを特徴とする。
本発明に係る医療材料としては、例えば、止血材料が挙げられる。
The food according to the present invention is characterized by containing the natural polymer compound composition described above.
The medical material according to the present invention is characterized by including the above-mentioned natural polymer compound composition.
Examples of the medical material according to the present invention include a hemostatic material.
本発明によれば、ポリリジンや油脂などを使用せずに、粘弾性を有する天然高分子化合物組成物、その製造方法、食品および医療材料を提供することができる。 ADVANTAGE OF THE INVENTION According to this invention, the natural high molecular compound composition which has viscoelasticity, its manufacturing method, foodstuffs, and medical materials can be provided, without using polylysine, fats and oils, etc.
以下、本発明の実施の形態の天然高分子化合物組成物、その製造方法、食品および医療材料について説明する。
本発明の実施の形態の天然高分子化合物組成物は、多価アルコールまたはその誘導体を含む分子量３０，０００以上３００,０００以下の架橋した親水性天然高分子化合物を含む。
Hereinafter, a natural polymer compound composition of the embodiment of the present invention, a method for producing the same, a food and a medical material will be described.
The natural polymer compound composition of the embodiment of the present invention contains a crosslinked hydrophilic natural polymer compound containing a polyhydric alcohol or a derivative thereof and having a molecular weight of 30,000 or more and 300,000 or less.
多価アルコールまたはその誘導体は、水分を乾燥させると同時に気化してしまわないよう、沸点が１００℃以上のもの、より好ましくは１５０℃以上のもの、さらに好ましくは１７０℃以上のものが好ましい。多価アルコールまたはその誘導体としては、例えば、グリセリン、プロピレングリコール、ジグリセリン、トリグリセリン、テトラグリセリン、ペンタグリセリン、ヘキサグリセリン、ヘプタグリセリン、オクタグリセリン、ナノグリセリン、デカグリセリンおよびそれらの脂肪酸誘導体が挙げられる。 The polyhydric alcohol or its derivative preferably has a boiling point of 100 ° C. or higher, more preferably 150 ° C. or higher, and further preferably 170 ° C. or higher so that it will not vaporize at the same time when water is dried. Examples of the polyhydric alcohol or a derivative thereof include glycerin, propylene glycol, diglycerin, triglycerin, tetraglycerin, pentaglycerin, hexaglycerin, heptaglycerin, octaglycerin, nanoglycerin, decaglycerin and fatty acid derivatives thereof. .
但し、グリセリンの脂肪酸エステルの鎖長が長くなればなるほど、融点が上がることから、鎖長は短いほうが好ましい、グリセリンの脂肪酸エステルの鎖長は、炭素数が１８以下、より好ましくは１４以下、さらに好ましくは１２以下が好ましい。多価アルコールまたはその誘導体は、重合度１〜１０のオリゴグリセリンまたはその誘導体から成ることが特に好ましい。 However, the longer the chain length of the fatty acid ester of glycerin, the higher the melting point, so the shorter chain length is preferred. The chain length of the fatty acid ester of glycerin is 18 or less, more preferably 14 or less, It is preferably 12 or less. It is particularly preferable that the polyhydric alcohol or its derivative comprises oligoglycerin or its derivative having a degree of polymerization of 1 to 10.
親水性天然高分子化合物としては、コラーゲン、ゼラチン、コラーゲンペプチド、ヒアルロン酸、アルギン酸、キチン、キトサン、セルロース、ヒドロキシプロピルセルロース、化工でんぷんなどを例示することができる。特に、親水性天然高分子化合物として、水に溶解させることができ、加工性に優れることから、ゼラチンまたはコラーゲンペプチドを用いることが好ましく、さらに、抗原性を低減した分子量３０，０００以上３００，０００以下のゼラチンまたはコラーゲンペプチドから成ることがより好ましく、分子量３０，０００以上１００，０００以下が特に好ましい。 Examples of the hydrophilic natural polymer compound include collagen, gelatin, collagen peptide, hyaluronic acid, alginic acid, chitin, chitosan, cellulose, hydroxypropyl cellulose, modified starch, and the like. In particular, it is preferable to use gelatin or collagen peptide as the hydrophilic natural polymer compound because it can be dissolved in water and is excellent in processability. Furthermore, the molecular weight of 30,000 or more and 300,000 with reduced antigenicity is preferable. The gelatin or collagen peptide described below is more preferable, and a molecular weight of 30,000 or more and 100,000 or less is particularly preferable.
親水性天然高分子化合物は、水中で容易に溶解しないよう架橋処理がなされている。架橋方法は、加熱処理や紫外線照射、放射線照射などの物理的方法、グルタルアルデヒドやカルボジイミドなどの架橋剤を用いる化学的方法のいずれでもよいが、酵素を用いる方法が好ましい。酵素としては、安全性が高いことから、トランスグルタミナーゼが好ましい。
本発明の実施の形態の天然高分子化合物組成物は、ポリリジンや油脂などを使用せずに、水分のない状態で、高い粘弾性を有する。
The hydrophilic natural polymer compound is subjected to a crosslinking treatment so as not to be easily dissolved in water. The cross-linking method may be either a physical method such as heat treatment, ultraviolet irradiation, or radiation irradiation, or a chemical method using a cross-linking agent such as glutaraldehyde or carbodiimide, but a method using an enzyme is preferable. As the enzyme, transglutaminase is preferable because of its high safety.
The natural polymer compound composition according to the embodiment of the present invention has high viscoelasticity in the absence of water without using polylysine, fats and oils.
本発明の実施の形態の天然高分子化合物組成物は、全重量に対し、多価アルコールまたはその誘導体４８〜６８重量％、親水性天然高分子化合物２５〜４８重量％、８０から１３０Ｕ／ｇの架橋剤０．０２〜０．１３重量％を含むことが好ましい。架橋剤がトランスグルタミナーゼから成る場合、トランスグルタミナーゼを０．１５〜０．１３重量％を含むことが好ましい。この配合比率の場合、本発明の実施の形態の天然高分子化合物組成物は、ポリリジンや油脂などを使用せずに、水分のない状態で、高い粘弾性および吸着性を有する。この高い粘弾性および吸着性は、例えれば、もちを焼いた時の内側のべたべた感のような性質である。 The natural polymer compound composition according to the embodiment of the present invention contains a polyhydric alcohol or a derivative thereof in an amount of 48 to 68% by weight, a hydrophilic natural polymer compound in an amount of 25 to 48% by weight, and an amount of 80 to 130 U / g based on the total weight. It is preferable to include 0.02 to 0.13% by weight of the crosslinking agent. When the cross-linking agent consists of transglutaminase, it is preferable to contain transglutaminase in an amount of 0.15 to 0.13% by weight. In the case of this blending ratio, the natural polymer compound composition of the embodiment of the present invention has high viscoelasticity and adsorptivity in the absence of water without using polylysine, fats and oils. This high viscoelasticity and adsorptivity are, for example, properties such as an inside sticky feeling when the rice cake is baked.
分子量３０，０００以上３００,０００以下の架橋した親水性天然高分子化合物のみの場合、乾燥状態では非常に脆く、吸着性がない。それに対し、多価アルコールまたはその誘導体を含む分子量３０，０００以上３００,０００以下の架橋した親水性天然高分子化合物は、乾燥状態で粘性があり、多価アルコールまたはその誘導体を含ませることによりゲル強度が格段に向上する。特に、親水性天然高分子化合物がコラーゲン、ゼラチンまたはコラーゲンペプチドでトランスグルタミナーゼを含む場合、その効果は顕著である。多価アルコールまたはその誘導体と、ゼラチンまたはコラーゲンペプチドと、トランスグルタミナーゼとの組成物は、医療材料として用いたとき、生体組織の水分を吸水し、ヒドロゲル状になり、高い密着、圧着効果を奏する。 In the case of only the crosslinked hydrophilic natural polymer compound having a molecular weight of 30,000 or more and 300,000 or less, it is very brittle in a dry state and has no adsorptivity. On the other hand, a crosslinked hydrophilic natural polymer compound having a molecular weight of 30,000 or more and 300,000 or less containing a polyhydric alcohol or a derivative thereof is viscous in a dry state, and a gel is obtained by including the polyhydric alcohol or a derivative thereof. Strength is dramatically improved. In particular, when the hydrophilic natural polymer compound is collagen, gelatin or collagen peptide and contains transglutaminase, the effect is remarkable. The composition of polyhydric alcohol or its derivative, gelatin or collagen peptide, and transglutaminase, when used as a medical material, absorbs water of living tissue and becomes a hydrogel, which exhibits high adhesion and pressure bonding effects.
天然高分子化合物組成物は、水分を除去したものでも除去しないものでもよい。水分を除去する場合、９５重量％以上除去して水分が５重量％未満のものとすることができ、９９重量％以上除去して水分が１重量％未満のものとすることもできる。
天然高分子化合物組成物は、多孔質体から成ることが好ましく、乾燥した多孔質体から成ることが特に好ましい。多孔質体は、表面に吸水を容易にする微少な孔などが多数存在する構造であり、起泡または発泡による気泡を含んだ構造から成っても、編物、織物、不織布のような繊維構造から成ってもよい。多孔質にすることにより、水分を吸水した後のゲル強度が強化され、組成物の強度を上げることができる。また、多孔質体とすることにより、食品や生体組織に所定の期間、密着、固定させることができる。
The natural polymer compound composition may or may not have water removed. In the case of removing water, 95% by weight or more can be removed to make the water content less than 5% by weight, and 99% by weight or more can be removed to make the water content less than 1% by weight.
The natural polymer compound composition preferably comprises a porous body, and particularly preferably a dried porous body. The porous body has a structure in which a large number of minute pores that facilitate water absorption are present on the surface, and even if it has a structure containing bubbles due to foaming or foaming, May be made up. By making it porous, the gel strength after absorbing water can be enhanced and the strength of the composition can be increased. Further, by using a porous body, it can be brought into close contact with and fixed to food or living tissue for a predetermined period.
本発明の実施の形態の天然高分子化合物組成物の製造方法は、親水性天然高分子化合物を多価アルコールまたはその誘導体の水溶液に溶解させて親水性天然高分子化合物溶液を調製する溶液調製工程と、前記親水性天然高分子化合物溶液に架橋剤を混合して架橋反応させる架橋反応工程と、架橋反応させた前記親水性天然高分子化合物溶液を攪拌して泡を生じさせる起泡工程とを、有する。 A method for producing a natural polymer compound composition according to an embodiment of the present invention is a solution preparation step of preparing a hydrophilic natural polymer compound solution by dissolving a hydrophilic natural polymer compound in an aqueous solution of a polyhydric alcohol or a derivative thereof. And a cross-linking reaction step of mixing a cross-linking agent in the hydrophilic natural polymer compound solution to carry out a cross-linking reaction, and a foaming step of stirring the cross-linking hydrophilic natural polymer compound solution to generate bubbles. , Have.
溶液調整工程で、多価アルコールまたはその誘導体の水溶液の濃度は５〜５０重量％が好ましい。
架橋反応工程では、親水性天然高分子化合物溶液に架橋剤を０℃以上４０℃以下で混合して架橋反応させることが好ましい。多価アルコールまたはその誘導体と、親水性天然高分子化合物と、架橋剤との配合比率としては、前述の配合比率が好ましい。
In the solution adjusting step, the concentration of the aqueous solution of the polyhydric alcohol or its derivative is preferably 5 to 50% by weight.
In the crosslinking reaction step, it is preferable to mix the hydrophilic natural polymer compound solution with a crosslinking agent at 0 ° C. or higher and 40 ° C. or lower to cause a crosslinking reaction. The compounding ratio of the polyhydric alcohol or its derivative, the hydrophilic natural polymer compound, and the crosslinking agent is preferably the above-mentioned compounding ratio.
一般に、高分子化合物多孔質体の製造方法の一つとして、高分子化合物を水などの溶媒に溶解した後に、この溶液を凍結乾燥する方法がある。しかし、この操作のみでは気泡含有状態にはならない。 Generally, as one of the methods for producing a polymer compound porous body, there is a method in which a polymer compound is dissolved in a solvent such as water and then this solution is freeze-dried. However, this operation alone does not result in a bubble-containing state.
本発明の実施の形態の天然高分子化合物組成物の製造方法では、起泡工程後、親水性天然高分子化合物溶液を乾燥させる乾燥工程を有することが好ましい。乾燥工程では、親水性天然高分子化合物溶液から水分を９５重量％以上、特に９９重量％以上除去することが好ましい。乾燥方法としては、凍結真空乾燥が好ましい。起泡工程後の凍結乾燥により、気泡を含有した天然高分子化合物組成物を製造することができる。凍結乾燥は、起泡工程後、多孔質構造を維持するよう速やかに行い、凍結後、速やかに真空乾燥を行うことが好ましい。起泡工程では、泡を均質化するため、攪拌速度と攪拌時間等を調節することが好ましい。 The method for producing a natural polymer compound composition according to the embodiment of the present invention preferably has a drying step of drying the hydrophilic natural polymer compound solution after the foaming step. In the drying step, it is preferable to remove 95% by weight or more, especially 99% by weight or more of water from the hydrophilic natural polymer compound solution. Freeze vacuum drying is preferred as the drying method. A natural polymer compound composition containing bubbles can be produced by freeze-drying after the foaming step. Freeze-drying is preferably carried out immediately after the foaming step so as to maintain a porous structure, and vacuum-drying is carried out immediately after freezing. In the foaming step, it is preferable to adjust the stirring speed, stirring time and the like in order to homogenize the foam.
特に、親水性天然高分子化合物溶液をホモミキサーにより回転数５０００〜２００００ｒｐｍで５〜４０分間撹拌することが好ましい。この場合、天然高分子化合物組成物の多孔質体の孔径は、０．３μｍ〜１００μｍとなる。本発明の実施の形態の天然高分子化合物組成物は、孔径が１μｍ〜３０μｍの範囲のものが好ましい。孔径が０．３μｍより小さいと生体組織の水が天然高分子化合物組成物に吸い込まれにくく、天然高分子化合物組成物が切れやすくなる。一方、１００μｍを超えると、天然高分子化合物組成物の多孔質体の強度が下がり過ぎて破れやすくなる。 In particular, it is preferable to stir the hydrophilic natural polymer compound solution with a homomixer at a rotation speed of 5000 to 20000 rpm for 5 to 40 minutes. In this case, the pore diameter of the porous body of the natural polymer compound composition is 0.3 μm to 100 μm. The natural polymer compound composition according to the embodiment of the present invention preferably has a pore size in the range of 1 μm to 30 μm. If the pore size is smaller than 0.3 μm, the water of the biological tissue is hard to be absorbed into the natural polymer compound composition, and the natural polymer compound composition is easily broken. On the other hand, when it exceeds 100 μm, the strength of the porous body of the natural polymer compound composition is too low and the porous body tends to be broken.
本発明の実施の形態の食品は、前述の天然高分子化合物組成物を含む。本発明の実施の形態の食品は、天然高分子化合物組成物により、高い粘弾性のある独特の食感を有する。本発明の実施の形態の食品には、調味料、賦形剤、蛋白質、キレート剤、乳化剤、着色剤、酸味料、香料、その他の食品添加物が混合されてもよい。食品としては、例えば、グミやもち菓子、代替肉などが挙げられる。 The food according to the embodiment of the present invention includes the natural polymer compound composition described above. The food of the embodiment of the present invention has a unique texture with high viscoelasticity due to the natural polymer compound composition. The food of the embodiment of the present invention may be mixed with seasonings, excipients, proteins, chelating agents, emulsifiers, coloring agents, acidulants, flavors and other food additives. Examples of the food include gummy candy, mochi confectionery, and substitute meat.
本発明の実施の形態の食品は、しっかり噛むことができ、満腹感が得やすいことからダイエット食としての用途や、咀嚼力を鍛える咀嚼トレーニングとしての用途に適している。また、食品への吸着性を高めた配合比率とした場合には、品質保持材の用途もある。
本発明の実施の形態の医療材料は、前述の天然高分子化合物組成物を含む。医療材料としては、例えば、止血材料が挙げられる。本発明の実施の形態の医療材料は、天然高分子化合物組成物により、高い粘弾性を有する。
The food according to the embodiment of the present invention is suitable for use as a diet diet and for mastication training for strengthening masticatory power because it can be chewed firmly and a feeling of fullness is easily obtained. Further, when the blending ratio is such that the adsorptivity to food is increased, it is also used as a quality retention material.
The medical material according to the embodiment of the present invention includes the natural polymer compound composition described above. Examples of medical materials include hemostatic materials. The medical material of the embodiment of the present invention has high viscoelasticity due to the natural polymer compound composition.
以下、実施例を挙げて本発明を説明するが、本発明はこれらの実施例に限定されるものではない。 Hereinafter, the present invention will be described with reference to examples, but the present invention is not limited to these examples.
（実施例１）
グリセリン：ゼラチン粉末(ニッピ社製)：トランスグルタミナーゼ（味の素社製）を６：２：０．０１の組成重量比で準備した。ゼラチン粉末は、分子量３０，０００以上３００，０００以下のものを用いた。トランスグルタミナーゼは、酵素活性８６Ｕ／ｇのものを用いた。グリセリン：ゼラチン粉末：水の重量比が６：２：４の水溶液を作製し、この水溶液を２５℃に保ち、トランスグルタミナーゼを添加した。この混合水溶液を冷蔵庫に入れ、５℃で一昼夜反応させた。その後、ホモミキサーを用いて、回転数１８０００ｒｐｍで１０分間撹拌し、均質化した。撹拌後の溶液をシャーレに流延し、−８０℃の冷凍庫に６時間入れてゼラチン水溶液を凍結させた後、凍結乾燥機中で４８時間、凍結乾燥処理した。こうして、架橋ゼラチン多孔質体から成る天然高分子化合物組成物を作製した。
(Example 1)
Glycerin: gelatin powder (manufactured by Nippi): transglutaminase (manufactured by Ajinomoto Co.) were prepared at a composition weight ratio of 6: 2: 0.01. The gelatin powder used had a molecular weight of 30,000 or more and 300,000 or less. The transglutaminase used had an enzyme activity of 86 U / g. An aqueous solution having a weight ratio of glycerin: gelatin powder: water of 6: 2: 4 was prepared, the aqueous solution was kept at 25 ° C., and transglutaminase was added. This mixed aqueous solution was placed in a refrigerator and reacted at 5 ° C. overnight. Then, using a homomixer, the mixture was stirred at 18,000 rpm for 10 minutes to homogenize. The solution after stirring was cast on a petri dish, put in a freezer at −80 ° C. for 6 hours to freeze the gelatin aqueous solution, and then freeze-dried for 48 hours in a freeze dryer. Thus, a natural polymer compound composition composed of a crosslinked gelatin porous material was prepared.
（比較例１）
グリセリン：ゼラチン粉末(ニッピ社製)：トランスグルタミナーゼ（味の素社製）を０：２：０．０１の組成重量比で準備した点を除き、実施例１と同様の方法で架橋ゼラチン多孔質体を作製した。
(Comparative Example 1)
A crosslinked gelatin porous material was prepared in the same manner as in Example 1 except that glycerin: gelatin powder (manufactured by Nippi): transglutaminase (manufactured by Ajinomoto Co.) was prepared at a composition weight ratio of 0: 2: 0.01. It was made.
（比較例２）
グリセリン：ゼラチン粉末(ニッピ社製)：トランスグルタミナーゼ（味の素社製）を６：２：０の組成重量比で準備した点を除き、実施例１と同様の方法でゼラチン多孔質体を作製した。
(Comparative example 2)
A gelatin porous material was prepared in the same manner as in Example 1 except that glycerin: gelatin powder (manufactured by Nippi): transglutaminase (manufactured by Ajinomoto Co.) was prepared at a composition weight ratio of 6: 2: 0.
（実施例２）
グリセリン：ゼラチン粉末(ニッピ社製)：トランスグルタミナーゼ（味の素社製）を６：２：０．０１の組成重量比で準備した。グリセリン：ゼラチン粉末：水の重量比が６：２：４の水溶液を作製し、この水溶液を２５℃に保ち、トランスグルタミナーゼを添加した。この混合水溶液をシャーレに流延し、−８０℃の冷凍庫に６時間入れてゼラチン水溶液を凍結させた後、凍結乾燥機中で４８時間、凍結乾燥処理した。こうして、架橋ゼラチン多孔質体から成る天然高分子化合物組成物を作製した。
(Example 2)
Glycerin: gelatin powder (manufactured by Nippi): transglutaminase (manufactured by Ajinomoto Co.) were prepared at a composition weight ratio of 6: 2: 0.01. An aqueous solution having a weight ratio of glycerin: gelatin powder: water of 6: 2: 4 was prepared, the aqueous solution was kept at 25 ° C., and transglutaminase was added. This mixed aqueous solution was cast on a petri dish, put in a freezer at −80 ° C. for 6 hours to freeze the gelatin aqueous solution, and then freeze-dried for 48 hours in a freeze dryer. Thus, a natural polymer compound composition composed of a crosslinked gelatin porous material was prepared.
（実施例３）
グリセリン：ゼラチン粉末(ニッピ社製)：トランスグルタミナーゼ（味の素社製）を１．２：２：０．０１の組成重量比で準備した点を除き、実施例１と同様の方法で架橋ゼラチン多孔質体から成る天然高分子化合物組成物を作製した。
(Example 3)
Glycerin: Gelatin powder (manufactured by Nippi): Transglutaminase (manufactured by Ajinomoto Co.) in the composition weight ratio of 1.2: 2: 0.01 except that crosslinked gelatin porous material was prepared in the same manner as in Example 1. A natural polymer composition composed of the body was prepared.
（実施例４）
グリセリン：ゼラチン粉末(ニッピ社製)：トランスグルタミナーゼ（味の素社製）を１０：２：０．０１の組成重量比で準備した点を除き、実施例１と同様の方法で架橋ゼラチン多孔質体から成る天然高分子化合物組成物を作製した。
(Example 4)
Glycerin: Gelatin powder (manufactured by Nippi): Transglutaminase (manufactured by Ajinomoto Co.) was prepared in the same manner as in Example 1 except that a composition weight ratio of 10: 2: 0.01 was prepared. A natural polymer compound composition was prepared.
（実施例５）
グリセリン：ゼラチン粉末(ニッピ社製)：トランスグルタミナーゼ（味の素社製）を６：２：０．０２５の組成重量比で準備した点を除き、実施例１と同様の方法で架橋ゼラチン多孔質体から成る天然高分子化合物組成物を作製した。
(Example 5)
Glycerin: Gelatin powder (manufactured by Nippi): Transglutaminase (manufactured by Ajinomoto Co.) was prepared in the same manner as in Example 1 except that a composition weight ratio of 6: 2: 0.025 was prepared. A natural polymer compound composition was prepared.
（実施例６）
グリセリン：ゼラチン粉末(ニッピ社製)：トランスグルタミナーゼ（味の素社製）を６：２：０．００２の組成重量比で準備した点を除き、実施例１と同様の方法で架橋ゼラチン多孔質体から成る天然高分子化合物組成物を作製した。
(Example 6)
Glycerin: Gelatin powder (manufactured by Nippi): Transglutaminase (manufactured by Ajinomoto Co.) was prepared in the same manner as in Example 1 except that a composition weight ratio of 6: 2: 0.002 was prepared. A natural polymer compound composition was prepared.
（実施例７）
ゼラチン粉末(ニッピ社製)の代わりにペプチドコラーゲン(ニッピ社製：分子量３００００)を用いた点を除き、実施例２と同様の方法で架橋ゼラチン多孔質体から成る天然高分子化合物組成物を作製した。
(Example 7)
A natural polymer compound composition comprising a crosslinked gelatin porous material was prepared in the same manner as in Example 2 except that peptide collagen (Nippi: molecular weight 30,000) was used instead of gelatin powder (Nippi). did.
（実施例８）
グリセリン：ヒドロキシプロピルセルロース（ダウケミカル社製）：トランスグルタミナーゼ（味の素社製）を６：０．５：０．０１の組成重量比で準備した点を除き、実施例２と同様の方法で架橋天然高分子多孔質体から成る天然高分子化合物組成物を作製した。
(Example 8)
Glycerin: Hydroxypropyl cellulose (manufactured by Dow Chemical Co.): Transglutaminase (manufactured by Ajinomoto Co.) at a composition weight ratio of 6: 0.5: 0.01, except that a cross-linked natural product was prepared in the same manner as in Example 2. A natural polymer compound composition composed of a polymer porous body was prepared.
（比較例３）
ゼラチン粉末(ニッピ社製)の代わりにペプチドコラーゲン(ニッピ社製：分子量１００００以下)を用いた点を除き、実施例２と同様の方法で天然高分子化合物組成物を作製した。
(Comparative example 3)
A natural polymer compound composition was prepared in the same manner as in Example 2 except that peptide collagen (manufactured by Nippi; molecular weight: 10,000 or less) was used instead of gelatin powder (manufactured by Nippi).
（実施例９）
グリセリンの代わりにプロピレングリコールを用いた点を除き、実施例２と同様の方法で架橋ゼラチン多孔質体から成る天然高分子化合物組成物を作製した。
(Example 9)
A natural polymer compound composition comprising a crosslinked gelatin porous material was prepared in the same manner as in Example 2 except that propylene glycol was used instead of glycerin.
（比較例４）
グリセリンの代わりにエタノールを用いた点を除き、実施例２と同様の方法で天然高分子化合物組成物を作製した。
(Comparative example 4)
A natural polymer compound composition was prepared in the same manner as in Example 2, except that ethanol was used instead of glycerin.
（実施例１０）
グリセリンの代わりにデカグリセリンモノラウリン酸エステル（三菱ケミカルフーズ社製）を用いた点を除き、実施例２と同様の方法で架橋ゼラチン多孔質体から成る天然高分子化合物組成物を作製した。
(Example 10)
A natural polymer compound composition comprising a crosslinked gelatin porous material was prepared in the same manner as in Example 2 except that decaglycerin monolaurate (manufactured by Mitsubishi Chemical Foods) was used instead of glycerin.
（比較例５）
グリセリンの代わりにデカグリセリンオレイン酸エステルを用いた点を除き、実施例２と同様の方法で天然高分子化合物組成物を作製した。
(Comparative example 5)
A natural polymer compound composition was prepared in the same manner as in Example 2, except that decaglycerin oleic acid ester was used instead of glycerin.
実施例１〜６および比較例１，２でそれぞれ作製したものについて、乾燥時と吸水時の状態観察と物性評価を行った。その結果を表１に示す。 With respect to the products produced in each of Examples 1 to 6 and Comparative Examples 1 and 2, state observation and physical property evaluation were performed during drying and during water absorption. The results are shown in Table 1.
表１に示すとおり、比較例１〜５ではいずれも粘弾性がなく、脆かったり固まらなかったりしたのに対し、実施例１〜１０ではいずれも粘弾性があった。実施例１〜１０は、いずれもしっかり噛むことができ、ホルモン様の独特の食感を有していた。実施例１〜１０は、いずれも食品として食することができた。 As shown in Table 1, Comparative Examples 1 to 5 did not have viscoelasticity and were brittle or did not solidify, whereas Examples 1 to 10 had viscoelasticity. All of Examples 1 to 10 were able to chew firmly and had a unique hormone-like texture. All of Examples 1 to 10 could be eaten as food.
実施例１〜６および比較例１，２でそれぞれ作製したものについて、豚レバーへの吸着性試験を行った。
、実施例１〜６および比較例１，２でそれぞれ作製したものから１ｃｍ×１ｃｍの大きさの試験材料を準備し、豚レバーの切り身（２００ｇ程度）に対する吸着性を調べた。その結果を表２に示す。
The adsorbability test on pig liver was carried out for the products produced in Examples 1 to 6 and Comparative Examples 1 and 2, respectively.
A test material having a size of 1 cm × 1 cm was prepared from each of the materials produced in Examples 1 to 6 and Comparative Examples 1 and 2, and the adsorptivity of the pork liver fillet (about 200 g) was examined. The results are shown in Table 2.
表２に示すとおり、比較例１では吸着せず、比較例２では吸着しにくかったのに対し、実施例１では吸着しやすくかつ粘弾性を有し、実施例２では千切れるものの吸着性を有し、実施例４では吸着性を有し、千切れにくかった。但し、実施例３，５，６では吸着しにくかった。従って、生体組織への吸着性には、グリセリンとゼラチン粉末とトランスグルタミナーゼとの配合比率が関係していると考えられる。
As shown in Table 2, Comparative Example 1 did not adsorb, and Comparative Example 2 was difficult to adsorb, whereas Example 1 was easy to adsorb and had viscoelasticity. In Example 4, it had an adsorptive property and was hard to be cut into pieces. However, in Examples 3, 5 and 6, it was difficult to adsorb. Therefore, it is considered that the adsorbability to living tissues is related to the mixing ratio of glycerin, gelatin powder, and transglutaminase.
Claims (14)
前記親水性天然高分子化合物溶液に架橋剤を混合して架橋反応させる架橋反応工程と、
架橋反応させた前記親水性天然高分子化合物溶液を攪拌して泡を生じさせる起泡工程とを、
有することを特徴とする天然高分子化合物組成物の製造方法。 A solution preparation step of preparing a hydrophilic natural polymer compound solution by dissolving a hydrophilic natural polymer compound in an aqueous solution of a polyhydric alcohol or a derivative thereof,
A cross-linking reaction step in which a cross-linking reaction is carried out by mixing a cross-linking agent in the hydrophilic natural polymer compound solution,
A foaming step of stirring the hydrophilic natural polymer compound solution which has been cross-linked to generate bubbles,
A method for producing a natural polymer compound composition, which comprises:
The medical material according to claim 13, which is a hemostatic material.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021200741A1 (en) | 2020-03-30 | 2021-10-07 | 日東電工株式会社 | Conductive film, bioelectrode, and biosensor |
| US11951229B2 (en) | 2020-08-20 | 2024-04-09 | Aobakasei Kabushiki Kaisha | Liquid hemostatic medical material |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0286743A (en) * | 1988-09-21 | 1990-03-27 | Nippi Zerachin Kogyo Kk | Production of gelatin having high polymerization degree |
| JPH02171160A (en) * | 1988-12-23 | 1990-07-02 | Nippi:Kk | Preparation of fish fin-like food |
| JPH11169121A (en) * | 1997-12-12 | 1999-06-29 | Miyagi Prefecture | Molding product, cushioning material and food using bean husk and their production |
| JP2004534023A (en) * | 2001-05-10 | 2004-11-11 | クロダ・インターナショナル・パブリック・リミテッド・カンパニー | Gelatin substitute |
| JP2010521994A (en) * | 2006-12-15 | 2010-07-01 | ライフボンド リミテッド | Gelatin-transglutaminase hemostatic dressing and sealant |
| JP2012237083A (en) * | 2011-05-13 | 2012-12-06 | Kansai Univ | Fiber using aqueous solution containing gelatin and polyhydric alcohol, and dry spinning method of hollow fiber |
| JP2015051626A (en) * | 2013-08-08 | 2015-03-19 | 日東電工株式会社 | Release sheet and silicone resin sheet having release sheet |
| WO2017195198A1 (en) * | 2016-05-09 | 2017-11-16 | Lifebond Ltd. | Mesh-based in situ cross-linkable compositions |
-
2018
- 2018-10-05 JP JP2018189803A patent/JP7332125B2/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0286743A (en) * | 1988-09-21 | 1990-03-27 | Nippi Zerachin Kogyo Kk | Production of gelatin having high polymerization degree |
| JPH02171160A (en) * | 1988-12-23 | 1990-07-02 | Nippi:Kk | Preparation of fish fin-like food |
| JPH11169121A (en) * | 1997-12-12 | 1999-06-29 | Miyagi Prefecture | Molding product, cushioning material and food using bean husk and their production |
| JP2004534023A (en) * | 2001-05-10 | 2004-11-11 | クロダ・インターナショナル・パブリック・リミテッド・カンパニー | Gelatin substitute |
| JP2010521994A (en) * | 2006-12-15 | 2010-07-01 | ライフボンド リミテッド | Gelatin-transglutaminase hemostatic dressing and sealant |
| JP2012237083A (en) * | 2011-05-13 | 2012-12-06 | Kansai Univ | Fiber using aqueous solution containing gelatin and polyhydric alcohol, and dry spinning method of hollow fiber |
| JP2015051626A (en) * | 2013-08-08 | 2015-03-19 | 日東電工株式会社 | Release sheet and silicone resin sheet having release sheet |
| WO2017195198A1 (en) * | 2016-05-09 | 2017-11-16 | Lifebond Ltd. | Mesh-based in situ cross-linkable compositions |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021200741A1 (en) | 2020-03-30 | 2021-10-07 | 日東電工株式会社 | Conductive film, bioelectrode, and biosensor |
| US11951229B2 (en) | 2020-08-20 | 2024-04-09 | Aobakasei Kabushiki Kaisha | Liquid hemostatic medical material |
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