JP2020020743A - Fluid device kit, method for manufacturing fluid device, and fluid device - Google Patents

Abstract

To provide a fluid device which can be easily assembled and cause less leakage in the circulation process.SOLUTION: The fluid device kit includes a fluid device body and a valve capable of controlling the flow of the fluid in the fluid device to form the fluid device. The fluid device body includes: a first substrate and a second substrate joined to each other; a flow path in the shape of a trench in one surface of the first substrate, the flow path being covered by the second substrate; and a through-hole in the second substrate, the through-hole connecting to the flow path. The valve is a flexible member which can be inserted in the through-hole.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a fluid device kit, a method for manufacturing a fluid device, and a fluid device.

  In recent years, attention has been paid to the development of μ-TAS (Micro-Total Analysis Systems) for the purpose of increasing the speed, efficiency, and integration of tests in the field of in vitro diagnostics, or miniaturizing test devices, and the like. Active research is ongoing worldwide.

μ-TAS is superior to conventional testing equipment in that it can be measured and analyzed with a small amount of sample, can be carried around, and can be disposable at low cost.
Furthermore, it is attracting attention as a highly useful method when an expensive reagent is used or when a small number of multiple samples are tested.

  As a component of μ-TAS, a device including a loop-shaped flow path and a pump disposed on the flow path has been reported (Non-Patent Document 1). In this device, a plurality of solutions are injected into the loop-shaped flow path, and the plurality of solutions are mixed in the loop-shaped flow path by operating a pump.

Jong Wook Hong, Vincent Studer, Giao Hang, W French Anderson and Stephen R Quake, Nature Biotechnology 22, 435-439 (2004)

  According to a first embodiment, the device includes a device body provided with a flow path through which a fluid flows and a hole extending from the flow path, and a flexible valve that can be inserted into the hole. A fluid device kit is provided which is capable of controlling the flow of a fluid in the flow channel by being deformed while being inserted into the hole.

  According to a second aspect, there is provided a method of manufacturing a fluidic device using the fluidic device kit of the first embodiment, comprising the step of inserting the valve into the hole. You.

  According to a third embodiment, there is provided a device body provided with a flow path through which a fluid flows and a hole extending from the flow path, and a flexible valve inserted into the hole, wherein the valve is The fluid device is provided, wherein the fluid device is closed by being deformed, and the valve can be removed from the hole.

FIG. 1 is a front view of a fluid device according to an embodiment. FIG. 1 is a plan view schematically showing a fluid device according to an embodiment. Sectional drawing which follows the III-III line in FIG. Sectional drawing which follows the IV-IV line in FIG. Sectional drawing which shows the structure of the upper plate and the valve fitted in the said upper plate. FIG. 4 is a cross-sectional view showing a method of assembling the fluid device kit, which is a cross-sectional view showing a fluid device main body and a plurality of valves. FIG. 6 is a cross-sectional view illustrating a method of assembling the fluid device kit, and is a cross-sectional view illustrating a state of a first example in which a valve is fitted into the fluid device main body. It is sectional drawing which shows the assembly method of a fluid device kit, and is sectional drawing which shows the state of the 2nd example in which the valve | bulb and the plug part were fitted in the fluid device main body. The figure for demonstrating the method of 1st leak evaluation. The figure for demonstrating the method of 2nd leak evaluation. The perspective view of a 1st valve. Sectional drawing of the 1st valve which follows the IX-IX line of FIG. 9A. The perspective view of a 2nd valve. Sectional drawing of the 2nd valve which follows the XX line of FIG. 10A. The perspective view of a 3rd valve. FIG. 11B is a sectional view of the third valve taken along line XI-XI in FIG. 11A. Sectional drawing which expands and shows the principal part of an upper plate. Sectional drawing which shows a mode that the valve fitted in the upper plate has not produced displacement. Sectional drawing which shows the mode that the displacement which the valve fitted in the upper plate has produced. 9 is a graph showing the evaluation results of the initial displacement of the valve.

  Hereinafter, embodiments of a fluid device kit and a method of manufacturing a fluid device will be described with reference to the drawings. In addition, in the drawings used in the following description, in order to make the characteristics easy to understand, the characteristic portions may be enlarged for convenience, and the dimensional ratios and the like of the respective components are not necessarily the same as the actual ones. I can't.

(Fluid device)
FIG. 1 is a front view of a fluid device 1 according to one embodiment. FIG. 2 is a plan view schematically showing the fluid device 1. In FIG. 2, the transparent upper plate 6 is illustrated in a state where the respective components disposed on the lower side are transmitted therethrough.

  The fluid device 1 of the present embodiment includes a device that detects a sample substance to be detected contained in a specimen sample by an immune reaction, an enzyme reaction, or the like. The sample substance is, for example, a biological molecule such as nucleic acid, DNA, RNA, peptide, protein, extracellular endoplasmic reticulum, and the like.

  The fluid device 1 includes a fluid device main body (device main body) 41, a septum 3, a plurality of valves V, Vi, Vo, and a pump P, as shown in FIG.

  As shown in FIGS. 1 and 2, the fluid device body 41 includes a base material 5 having at least a flow channel 11 through which a fluid flows and a plurality of valve holding holes (through holes) 34 extending from the flow channel 11. Become. The width and depth of the flow channel 11 are preferably 0.1 mm to several tens mm. For example, the channel width is 1.5 mm and the depth is 0.3 mm.

  The base material 5 has an upper plate (first substrate) 6, a lower plate (third substrate) 8, and a substrate (second substrate) 9. The upper plate 6, the lower plate 8, and the flow path substrate 9 of the present embodiment are made of a resin material. Examples of the resin material constituting the upper plate 6, the lower plate 8, and the flow path substrate 9 include polypropylene, polycarbonate, and the like. In the present embodiment, the upper plate 6 and the lower plate 8 are made of a transparent material. In addition, the material which comprises the upper plate 6, the lower plate 8, and the flow path substrate 9 is not limited.

  In the following description, an upper plate (eg, a lid, an upper or lower part of a channel, an upper or lower surface of a channel) 6, a lower plate (eg, a lid, an upper or lower part of a channel, an upper surface of a channel, The bottom plate 8 and the flow path substrate 9 are arranged along a horizontal plane, the upper plate 6 is disposed above the flow path substrate 9 and the lower plate 8 is disposed below the flow path substrate 9. However, this merely defines the horizontal direction and the vertical direction for convenience of description, and does not limit the orientation when the fluid device 1 according to the present embodiment is used.

  The upper plate 6, the flow path substrate 9, and the lower plate 8 are plate members extending along the horizontal direction. The upper plate 6, the lower plate 8, and the flow path substrate 9 are each manufactured by cutting or injection molding. The upper plate 6, the flow path substrate 9, and the lower plate 8 are stacked in this order along the vertical direction. The flow path substrate 9 is joined to the upper plate 6 with its second surface 9b facing the first surface 6a of the upper plate 6. The lower plate 8 is joined to the flow path substrate 9 with its second surface 8b facing the first surface 9a of the flow path substrate 9. The fluid device main body 41 is composed of the base material 5 manufactured by joining the upper plate 6, the lower plate 8, and the flow path substrate 9 by joining means such as laser welding and integrating them.

  In the following description, the direction in which the upper plate 6, the flow path substrate 9, and the lower plate 8 are stacked is simply referred to as a stacking direction. In the present embodiment, the laminating direction is a vertical direction.

  As shown in FIG. 1, the upper plate 6 is provided with a plurality of first through holes (through holes) 37, air holes 35, and a plurality of valve holding holes 34. The first through hole 37, the air hole 35, and the valve holding hole 34 penetrate the upper plate 6 in the plate thickness direction.

  The first through-hole 37 is located immediately above the second through-hole 38 of the flow path substrate 9 and is connected to the second through-hole 38 as described later. That is, the first through-hole 37 and the second through-hole 38 overlap with each other when viewed from the stacking direction. The first through hole 37 and the second through hole 38 form the injection hole 32. The first through hole 37 forms an opening of the injection hole 32. That is, the opening of the injection hole 32 is located in the upper plate 6.

  The air hole 35 is located on the upper plate 6 immediately above the waste liquid tank 7. The air hole 35 connects the waste liquid tank 7 to the outside. As will be described later, a suction device (negative pressure applying device: not shown) can be connected to the air hole 35.

  The valve holding hole 34 holds the valves V, Vi, Vo, and a plurality of pump valves Pa, Pb, Pc constituting the pump P.

  The valves V, Vi, Vo and the pump valves Pa, Pb, Pc are flexible valves that can be attached to and detached from the valve holding holes 34. The valves V, Vi, Vo and the pump valves Pa, Pb, Pc are configured to close the flow path 11 (FIG. 2) provided between the upper plate 6 and the flow path substrate 9. That is, the valves V, Vi, Vo and the pump valves Pa, Pb, Pc are deformed while being inserted into the valve holding holes 34, so that the flow of the fluid in the flow path 11 can be controlled.

  As shown in FIG. 2, each of the valves V, Vi, and Vo is provided in the path of the circulation flow path 10 and divides the circulation flow path 10 into, for example, three quantitative sections 18. These valves V, Vi, and Vo are arranged so that each of the fixed quantity sections 18 of the circulation flow path 10 has a predetermined volume.

  The pump P includes three pump valves Pa, Pb, and Pc arranged side by side in the flow path. The pump P can transport a solution (fluid) in the circulation channel 10 by sequentially opening and closing the pump valves Pa, Pb, and Pc. The number of valves constituting the pump valves Pa, Pb, Pc may be four or more.

FIG. 3 is a cross-sectional view of the fluid device 1 along the line III-III in FIG.
As shown in FIG. 3, the flow path substrate 9 has a first surface 9a and a second surface 9b. The upper plate 6 is located on the second surface 9 b side of the flow path substrate 9. The lower plate 8 is located on the first surface 9 a side of the flow path substrate 9.

  The flow path substrate 9 includes a reservoir layer 19A on the first surface 9a side. A plurality of reservoirs 29 are provided on the reservoir layer 19A. Further, the flow path substrate 9 includes a reaction layer 19B on the second surface 9b side. The flow channel 11 and the waste liquid tank 7 are provided in the reaction layer 19B.

  As shown in FIG. 2, at least a part of the flow channel 11 and at least a part of the reservoir 29 are arranged so as to overlap each other when viewed from the laminating direction. According to the present embodiment, by disposing the flow path 11 or the reservoir 29 on the first surface 9a side and the second surface 9b side of the flow path substrate 9, respectively, the flow path 11 and the reservoir 29 are viewed from the laminating direction. Can be placed one on top of the other. Thereby, the fluid device 1 can be downsized.

As shown in FIGS. 3 and 4, the flow path substrate 9 is provided with a supply hole 39 and a second through hole 38 penetrating vertically.
The supply hole 39 connects the reservoir 29 and the flow path 11. The solution stored in the reservoir 29 is supplied to the flow channel 11 through the supply hole 39.

FIG. 4 is a cross-sectional view of the fluid device 1 along the line IV-IV in FIG.
The second through-hole 38 is connected to the first through-hole 37 of the upper plate 6 to form the injection hole 32. The injection hole 32 connects the reservoir 29 to the outside. The solution is filled into the reservoir 29 through the injection hole 32.

  The septum 3 is fixed to the first through hole 37. The septum 3 closes the opening of the injection hole 32.

  In the fluid device main body 41, a valve holding hole 34 is formed at a position where the valve V is assumed to be arranged. Then, according to the arrangement of the flow path 11, the valve V is inserted into the valve holding hole 34 used as the valve V, and the septum 3 is inserted into the valve holding hole 34 not used as the valve V.

(Valve structure)
FIG. 5 is a sectional view showing the configuration of the upper plate 6 and the valves V (Vi, Vo...) Fitted into the valve holding holes 34 of the upper plate 6.
The valves constituting the valves V, Vi, Vo and the pump valves Pa, Pb, Pc have the same structure. Therefore, the structure of the valve V will be described below as an example.

  As shown in FIG. 5, the valve V has a bottom portion 61 formed of a film portion, a cylindrical portion 62, and a flange portion 63, and has a circular shape in plan view as viewed from the center axis direction. The valve holding hole 34 formed in the upper plate 6 has a size capable of accommodating the valve V, has a penetrating portion 34a and an expanding portion 34b, and has a circular shape in plan view when viewed from the central axis direction. Present. Therefore, when the valve V is inserted into the valve holding hole 34 of the upper plate 6, the cylindrical portion 62 is housed in the through portion 34a of the valve holding hole 34, and the flange portion 63 is housed in the expansion portion 34b. You.

  The bottom portion 61 is a portion that functions as a diaphragm and is capable of controlling the flow of the solution by being deformed in the thickness direction so as to close the flow channel 11 shown in FIG. The thickness of the bottom 61 is, for example, in the range of 0.1 mm to 1.0 mm, and preferably 0.3 mm.

  The cylindrical portion 62 is a portion extending from the outer peripheral portion of the bottom portion 61 to one side in the thickness direction of the bottom portion 61. The flange portion 63 is an annular flange that is located at an end opposite to the bottom portion 61 of the tubular portion 62 and extends radially outward in the radial direction of the tubular portion 62.

  The flange portion 63 has an upper surface 63a facing one side in the thickness direction of the bottom portion 61. The upper surface 63a is provided with an annular packing portion 63A protruding from the second surface 6b of the upper plate 6 while being attached to the upper plate 6, and a flat portion 63B formed of a surface parallel to the bottom portion 61. The packing part 63A is located radially outside the flat part 63B. The packing portion 63A protruding from the second surface 6b of the upper plate 6 functions as an O-ring.

  The diameter D1 of the through portion 34a in the valve holding hole 34 is, for example, ± 0.2 mm or less with respect to the diameter d1 of the cylindrical portion 62 of the valve V. The outer diameter D2 of the expansion portion 34b is, for example, ± 0.2 mm or less with respect to the outer diameter d2 of the flange portion 63 of the valve V. The depth T of the extension portion 34b, that is, the depth T from the second surface 6b of the upper plate 6 to the counterbore surface 6c is, for example, about 0.3 mm.

(Method of Manufacturing Fluid Device 1)
FIG. 6A is a cross-sectional view showing a method of assembling the fluid device kit 100, and is a cross-sectional view showing the fluid device main body 41 and a plurality of valves V (Vi, Vo). FIG. 6B is a cross-sectional view illustrating the method of assembling the fluid device kit 100, and is a cross-sectional view illustrating a state of the first example in which the valves Vi and Vo are fitted into the fluid device body 41. FIG. 6C is a cross-sectional view illustrating a method of assembling the fluid device kit 100, and is a cross-sectional view illustrating a state of the second example in which the valve Vi and the stopper S are fitted into the fluid device main body 41. 6A, 6B, and 6C are views schematically showing the cross-sectional view shown in FIG.

  As shown in FIG. 6A, the fluid device kit 100 includes a fluid device main body 41 made of the base material 5, a plurality of valves V (in FIGS. 6A and 6B, for example, valves Vi and Vo are shown), and a stopper S. And

In the method of manufacturing the fluid device 1, the operator inserts the valve V (Vi, Vo and the pump valve) from the second surface 6 b side of the upper plate 6 of the fluid device main body 41 into the plurality of valve holding holes 34 of the upper plate 6. Pa, Pb, and Pc (not shown in FIGS. 6A and 6B) are fitted respectively. At this time, each valve V is merely fitted into the valve holding hole 34 of the upper plate 6 and does not adhere to the upper plate 6.
Thus, the fluidic device 1 of the present embodiment as shown in FIG. 6B is manufactured.

  In the present embodiment, a large number of valves V are mass-produced by injection molding in advance, and a plurality of separately manufactured valves V are fitted into the respective valve holding holes 34 of the fluid device main body 41, so that the fluid device 1 can be simplified. Can be assembled. In addition, since the adhesive is not required by using the valve fitting type, there is no possibility that the adhesive enters the flow channel 11.

  Further, since each valve V is independent of the fluid device main body 41, it is possible to immediately respond to a slight design change on the fluid device main body 41 side. In other words, it is possible to cope with the problem by simply shaving the base material 5 without newly preparing a mold. For this reason, the fluid device kit 100 of the present embodiment is suitable not only for trial production but also for production of a small number and variety of fluid devices 1.

  As shown in FIGS. 6A and 6C, the fluid device kit 100 of the present embodiment has a plug portion S that can be inserted into a through hole provided in the upper plate 6. The through hole into which the stopper S is fitted is, for example, the valve holding hole 34. In the method of manufacturing the fluid device 1, the operator can insert the plugs S into some of the plurality of valve holding holes 34 as necessary.

The plug S closes the through hole by being inserted into a through hole provided in the upper plate 6, for example, the valve holding hole 34. For example, the fluid for driving the valve can be prevented from leaking from the valve holding hole 34 by being inserted into the valve holding hole 34 that does not require valve driving. In this case, since the fluid device kit 100 has the valve V and the plug S, and the fluid device 1 has the plurality of valve holding holes 34, the valve V or the plug S can be inserted into an arbitrary valve holding hole 34. And the degree of freedom of valve arrangement in the flow path 11 increases.
When filling the flow path with the solid reagent or the viscous reagent, the solid reagent or the viscous reagent is filled into the flow path 11 through the through-hole provided in the upper plate 6, and then the stopper S is fitted into the through-hole. Is also possible. This method is effective when there are restrictions on the reagent filling direction.
The plug S may be inserted into the valve holding hole 34 to close a part of the flow channel 11 of the fluid device main body 41. In this case, the stopper S restricts passage of the solution in the flow path 11. By providing the stopper S, intrusion of the solution into a predetermined region of the flow channel 11 is suppressed. Therefore, since the fluid device kit 100 has the stopper S, the operator can select which of the plurality of quantitative sections 18 to use depending on the type of the solution to be used and the degree of dilution. As an example, it is assumed that the fluid device main body 41 is provided with two quantitative sections that branch off from each other. The volumes of the two metering compartments are different from each other. When assembling the fluid device 1, the operator closes both ends of one of the fixed amount sections with the stopper S. Thereby, the fluid device 1 for introducing the solution into only one of the fixed amount sections can be configured.

  The stopper S has, for example, a cylindrical shape with one end closed. The outer diameter of the bottom of the stopper S and the outer diameter of the bottom 61 of the valve V are equal to each other. The diameter of the through portion 34a of the fluid device main body 41 is, for example, ± 0.2 mm or less with respect to the outer diameter of the plug S. The thickness of the bottom of the plug S is greater than the thickness of the bottom 61 of the valve V. Thus, even when the plurality of valve holding holes 34 have a common valve driving fluid flow path, the stopper S is not deformed, and only the valve V can be deformed.

(Leak evaluation)
Next, a leak evaluation for the manufactured fluid device 1 will be described.
In the present embodiment, the plurality of valves V are merely fitted into the fluid device main body 41 (the upper plate 6 of the base member 5), but are not bonded. For this reason, the leak evaluation was performed with the valve V fitted in the fluid device body 41.

FIG. 7 is a diagram for explaining a first leak evaluation method. FIG. 8 is a diagram for explaining a second leak evaluation method.
Here, as shown in FIG. 7, the first leak evaluation by sending air from the packing portion 63A side of each valve V1, V2, V3, and as shown in FIG. 8, the bottom of each valve V1, V2, V3 A second leak evaluation was performed by feeding air from the 61 side.

  In performing each leak evaluation for the valves V1, V2, and V3, eight patterns of the first upper plate 6A to the eighth upper plate 6H in which the design conditions of the valve holding holes 34 are different were prepared. Further, the valves V1, V2, and V3 have different outer shapes.

  FIGS. 9A and 9B to 11A and 11B are diagrams showing three patterns of valves V1, V2 and V3 having different external shapes. 9A is a perspective view of the first valve V1, and FIG. 9B is a cross-sectional view of the first valve V1 along line IX-IX in FIG. 9A. FIG. 10A is a perspective view of the second valve V2, and FIG. 10B is a cross-sectional view of the second valve V2 along line XX in FIG. 10A. FIG. 11A is a perspective view of the third valve V3, and FIG. 11B is a cross-sectional view of the third valve V3 along line XI-XI in FIG. 11A. FIG. 12 is an enlarged cross-sectional view showing a main part of the upper plate 6.

  The first valve V1 shown in FIGS. 9A and 9B has a configuration in which a flat portion 63B is provided in the flange portion 63 radially inside the packing portion 63A. The second valve V2 shown in FIGS. 10A and 10B has the same configuration as that of the above-described embodiment, and has a configuration in which a flat portion 63B is provided radially outside the packing portion 63A in the flange portion 63. I have. In the third valve V3 shown in FIGS. 11A and 11B, the packing portion 63A is provided over the entire width of the flange portion 63, and the flat portion 63B does not exist.

  Regarding the sizes of the valves V1, V2, and V3, the diameter d1 of the cylindrical portion 62 is 3.2 mm, and the outer diameter d2 of the flange portion 63 is 4.0 mm. Further, the volume of the packing portion 63A in each of the valves V1, V2, V3 is also common. In each of the valves V1, V2, and V3, the thickness of the bottom portion 61 is 0.3 mm, and the thickness of the cylindrical portion 62 is also 0.3 mm.

  As shown in FIG. 12, in the first upper plate 6A, the diameter D1 of the through portion 34a of the valve holding hole 34 is 3.1 mm, and the outer diameter D2 of the expanded portion 34b is 4.0 mm. In the second upper plate 6B, the diameter D1 of the through portion 34a of the valve holding hole 34 is 3.1 mm, and the outer diameter D2 of the expansion portion 34b is 4.4 mm. In the third upper plate 6C, the diameter D1 of the through portion 34a of the valve holding hole 34 is 3.2 mm, and the outer diameter D2 of the expansion portion 34b is 4.0 mm. In the fourth upper plate 6D, the diameter D1 of the through portion 34a of the valve holding hole 34 is 3.2 mm, and the outer diameter D2 of the expansion portion 34b is 4.4 mm. In the fifth upper plate 6E, the diameter D1 of the through portion 34a of the valve holding hole 34 is 3.3 mm, and the outer diameter D2 of the expanded portion 34b is 4.0 mm. In the sixth upper plate 6F, the diameter D1 of the through portion 34a of the valve holding hole 34 is 3.3 mm, and the outer diameter D2 of the expansion portion 34b is 4.4 mm. In the seventh upper plate 6G, the diameter D1 of the through portion 34a of the valve holding hole 34 is 3.4 mm, and the outer diameter D2 of the expanded portion 34b is 4.0 mm. In the eighth upper plate 6H, the diameter D1 of the through portion 34a of the valve holding hole 34 is 3.4 mm, and the outer diameter D2 of the expanded portion 34b is 4.4 mm.

  Differences in the design conditions of the above-described upper plates 6A to 6H (bulb holding hole 34: diameter D1 of penetrating portion 34a, outer diameter D2 of expansion portion 34b) are summarized in Table 1 below. In Table 1, the diameters D1 and D2 of the valves V1, V2 and V3 are "narrow" when the diameters D1 and D2 of the valve holding holes 34 are narrow, "wide" when they are wide, and "fit" when they are the same. ".

<Leak evaluation result>
Next, each of the first leak evaluation (leak evaluation from the packing portion 63A side of the valves V1, V2, and V3) and the second leak evaluation (leak evaluation from the bottom 61 side of the valves V1, V2, and V3) The results will be described.

  In each of the leak evaluations for the three types of valves V1, V2, and V3, the pressure was increased from the atmospheric pressure in increments of 50 kPa, and a pressure value with no pressure fluctuation was recorded for one minute in a state where the depressurization was cut off. Table 1 summarizes the results of each leak evaluation.

  As shown in Table 1, when the hole shape on the upper plate 6 side is smaller than the shape of each valve V1, V2, V3 (the diameter d1 of the cylindrical portion 62> the diameter D1 of the through portion 34a), In both the "first leak evaluation" and the "second leak evaluation", a withstand voltage of 250 kPa or more was confirmed.

  Further, in any of the valves V1, V2, and V3, it is suggested that the "second leak evaluation" has a higher withstand pressure than the "first leak evaluation" and can sufficiently withstand the water pressure from the flow path 11 side. Was done. Among them, a shape in which the packing portion 63A is partially provided has a higher withstand voltage than a shape in which the packing portion 63A is provided on the entire flange portion 63 of the valves V1, V2, and V3. It became. In particular, when using the second valve V2 having a shape in which the packing portion 63A is positioned radially inward of the flat portion 63B, in each of the "first leak evaluation" and the "second leak evaluation", 250 kPa or more is used. High pressure resistance was obtained. In the second valve V2, the load vertically applied to the packing portion 63A functioning as an O-ring is strong, and thus it is considered that the second valve V2 effectively acts on the leak.

  From the above results, it is preferable to use the second valve V2 as the shape of the valve used for the fluid device 1. The dimension of the valve holding hole 34 formed in the upper plate 6 is such that the shape of the valve holding hole 34 fits (D1 = d1, D2 = d2) with the shape of the valve V to be fitted, or is smaller than that. It is preferable that the dimensions are narrow (D1 <d1, D2 <d2).

(Initial change of valve V)
Next, the initial displacement of the valve V was evaluated.
FIG. 13 is a cross-sectional view showing a state in which no displacement occurs in the valve V1 fitted in the upper plate 6. FIG. 14 is a cross-sectional view showing a state in which the valve V1 fitted to the upper plate 6 is displaced.

As shown in FIG. 13, when no displacement occurs in the valve V1 fitted in the upper plate 6, the bottom portion 61 of the valve V1 is substantially flat.
However, as shown in FIG. 14, the valve V1 fitted into the valve holding hole 34 of the upper plate 6 is crushed by, for example, coming into contact with the flow path substrate 9 and the manifold 12, so that the bottom of the valve V1 61 may be deformed. In some cases, since the bottom portion 61 of the valve V1 is displaced outward (to the side opposite to the flange portion 63) in a convex manner from the beginning when the valve V1 is fitted into the upper plate 6, the flow path 11 is already formed before use. It may be obstructed. For this reason, in order to reduce the initial displacement of the valve V1 fitted into the upper plate 6, it is necessary to consider the design conditions of the upper plate 6 (the dimensions of the valve holding hole 34).

Here, the initial deformation was evaluated using the first valve V1. The initial displacement amount of the first valve V1 was measured in a state where the first valve V1 was fitted in each of the first upper plate 6A to the eighth upper plate 6H having different design conditions of the valve holding hole 34.
Regarding the size of the first valve V1, the diameter d1 of the cylindrical portion 62 is 3.2 mm, and the outer diameter d2 of the flange portion 63 is 4.0 mm.

FIG. 15 is a graph showing an evaluation result of the initial displacement of the first valve V1.
Like the first upper plate 6A to the third upper plate 6C, when the size of the through portion 34a in the valve holding hole 34 is the same as or smaller than the size of the cylindrical portion 62 of the first valve V1, each upper plate 6 The initial displacement amount of the fitted first valve V1 was large, and a displacement of 200 μm or more was observed in each case.

  On the other hand, when the size of the expanded portion 34b of the valve holding hole 34 is larger than the size of the flange portion 63 of the first valve V1, like the fourth upper plate 6D, the initial displacement of the fitted first valve V1 is performed. The amount was reduced to about 55 μm. As shown in FIG. 15, the displacement amount of the valve V1 is considerably suppressed as compared with the case where the flange portion 63 is the third upper plate 6C that fits the extension portion 34b.

  Further, when the diameter D1 of the penetrating portion 34a in the valve holding hole 34 is equal to or larger than the diameter d1 of the cylindrical portion 62 of the valve V1 as in the fifth upper plate 6E and the sixth upper plate 6F. Regardless of the size of the extension portion 34b with respect to the flange portion 63, the displacement amount of the valve V1 is as small as about 50 μm.

  In the seventh upper plate 6G and the eighth upper plate 6H, the diameter D1 of the through portion 34a in the valve holding hole 34 is larger than the diameter d1 of the cylindrical portion 62 of the valve V1. When the outer diameter D2 of the extension portion 34b is the same as the outer diameter d2 of the flange portion 63 of the valve V1 as in the case of the seventh upper plate 6G, the bottom portion 61 of the valve V1 is recessed toward the flange portion 63. However, the displacement amount is as small as 50 μm.

  On the other hand, in the eighth upper plate 6H, the outer diameter D2 of the expanded portion 34b is larger than the outer diameter d2 of the flange portion 63 of the valve V1, and the valve holding hole 34 is formed larger than the valve V1. . Therefore, the valve V1 receives little stress from the eighth upper plate 6H, and the bottom portion 61 of the valve V1 is hardly deformed.

  As described above, as the diameter D1 of the through portion 34a of the valve holding hole 34 is larger than the diameter d1 of the cylindrical portion 62 of the valve V (d1 <D1), the stress that the valve V1 receives from the upper plate 6 is smaller. The initial displacement of the bottom 61 of the valve V1 is small. Further, as the outer diameter D2 of the expanded portion 34b of the valve holding hole 34 is larger than the outer diameter d2 of the flange portion 63 of the valve V (d2 <D2), the displacement is reduced on the flange portion 63 side of the valve V1. It was found that the initial displacement of the bottom portion 61 in the valve V1 was small.

  In particular, the initial displacement of each valve V1 in the third upper plate 6C and the fourth upper plate 6D in which the size of the cylindrical portion 62 of the valve V1 fits the penetrating portion 34a of the valve holding hole 34 is compared. In addition, the fourth upper plate 6D in which the outer diameter d2 of the extension portion 34b is larger than the flange portion 63 has a larger valve than the third upper plate 6C in which the flange portion 63 fits the extension portion 34b. It can be seen that the displacement of V1 is considerably small, and the difference is remarkable. As described above, the larger the diameter D1 of the through portion 34a of the valve holding hole 34 with respect to the size of the valve V, the smaller the initial displacement of the valve V can be.

  However, if the size of the valve holding hole 34 is too large with respect to the valve V, the withstand voltage against leakage is reduced. In view of this, it is necessary as a design condition on the upper plate 6 side to obtain a high withstand voltage against leakage while suppressing the initial displacement amount of the valve V.

  In this embodiment, as shown in Table 1, when the second valve V2 is used as the valve V and the fifth upper plate 5E is used as the upper plate 6, either the O-ring (packing portion) side or the flow path side is used. Also obtained a high withstand voltage of 250 kPa or more. Therefore, when the second valve V2 is used, as a design condition on the upper plate 6 side, the diameter D1 of the through portion 34a of the valve holding hole 34 is 3.2 mm, and the outer diameter D2 of the expansion portion 34b is 2.0 mm. It is preferable to use the fifth upper plate 5E. In the fifth upper plate 5E, the through portion 34a of the valve holding hole 34 is smaller than the cylindrical portion 62 of the valve V2 by about 0.1 mm, and the flange portion 63 is dimensioned to fit the expanded portion 34b. . As a result, a high withstand voltage against leakage was obtained, and the initial displacement of the second valve V was reduced. It is preferable that the initial displacement of the valve V is small in order to suppress the influence on the fraction quantification.

  According to the fluid device 1 of the present embodiment, even if the fluid device body 41 is assembled by fitting a plurality of independent valves V into the fluid device main body 41, the fluid device 1 can be used as a valve function by the above-described two leak evaluations. Since sufficient pressure resistance was obtained, it is possible to manufacture the fluid device 1 having pressure resistance close to the two-color molding used in the conventional manufacturing method.

  In addition, by adopting a configuration in which the initial deformation amount of the valve V when the valve V is fitted into the upper plate 6, a fractionation quantitative performance equivalent to a device manufactured by two-color molding can be obtained.

  Further, by preparing a plurality of valves V, it is possible to use different types of valves V according to the pressure to be applied.

  Further, it is also possible to remove the valve V and the septum 3 from the fluid device main body 41 and collect the solution inside the substrate 5.

  The fluid device 1 can be reused by removing the valve V and the septum 3 from the fluid device main body 41 and cleaning the channel 11 and the like.

  Although various embodiments of the present invention have been described above, each configuration and a combination thereof are merely examples, and addition, omission, substitution, and other changes of the configuration may be made without departing from the spirit of the present invention. It is possible. The present invention is not limited by the embodiments.

  For example, in the above-described embodiment, the configuration is such that all the valves V are fitted into the fluid device main body 41 later. However, at least one valve V is of a fitting type, and the other valves V are different from those in the related art. Similarly, it may be configured to be integrally formed by two-color molding, insert molding, injection molding or the like.

  Further, the shapes of the valves V fitted into the fluid device main body 41 do not have to be all the same.

  Further, the fluid device main body 41 of the fluid device kit 100 may further include a detection unit capable of detecting a substance in the solution. Thus, after assembling the fluid device 1 using the fluid device kit 100, the substance in the solution is detected, and the valve V can be removed from the fluid device main body 41 after the detection.

  Reference Signs List 1 fluid device, 3 septum, 6 upper plate (first substrate), 6b second surface (front surface), 8 lower plate (third substrate), 9 flow path substrate (second substrate) ), 11 ... flow path, 34 ... valve holding hole (through hole), 34a ... penetrating part, 34b ... expansion part, 41 ... fluid device main body (device main body), 61 ... bottom part (membrane part), 62 ... cylindrical part 63, a flange portion, 63a, a top surface, 63A, a packing portion, 63B, a flat portion, 100, a fluid device kit, D1, a diameter of the penetrating portion 34a, D2, an outer diameter of the expansion portion 34b, d1, a cylindrical portion 62 Diameter, d2: outer diameter of flange 63, V, V1, V2, V3, Vi: valve, Pa, Pb, Pc: pump valve (valve), S: stopper

Claims (9)

Fluid device body, comprising a valve capable of controlling the flow of the fluid of the fluid device, a fluid device kit constituting a fluid device,
The fluid device body has a first substrate and a second substrate joined to each other,
A flow path formed in a groove shape on one surface of the second substrate and formed by covering with the first substrate;
A through-hole provided in the first substrate so as to be connected to the flow path,
The valve is a flexible member that can be inserted into the through hole of the fluid device,
Fluid device kit. The valve is
A film part that can be deformed in the thickness direction,
A cylindrical portion extending from the peripheral portion of the film portion to one side in the thickness direction of the film portion;
An annular flange portion located at an end of the cylindrical portion opposite to the film portion and extending radially outward of the cylindrical portion,
The through hole,
A cylindrical portion, a through portion capable of accommodating the tubular portion of the valve,
An extending portion that is extended radially outward of the through portion and that accommodates the flange portion of the valve.
The fluid device kit according to claim 1. The flange portion has an upper surface facing one side in the thickness direction of the film portion,
On the top surface,
An annular packing portion protruding from the surface of the device main body while being attached to the device main body is provided,
The fluid device kit according to claim 2. On the upper surface of the flange portion, a flat portion located radially outside the cylindrical portion with respect to the packing portion is provided.
The fluid device kit according to claim 3. In a plan view, the penetrating portion and the tubular portion are circular,
The diameter of the penetrating portion is ± 0.2 mm or less with respect to the diameter of the tubular portion.
The fluid device kit according to any one of claims 2 to 4. In a plan view, the expansion portion and the flange portion are annular,
The outer diameter of the expansion portion is ± 0.2 mm or less with respect to the outer diameter of the flange portion.
The fluid device kit according to any one of claims 2 to 5. Further comprising a plug that can be inserted into the through hole;
The fluid device kit according to any one of claims 1 to 6. A method for manufacturing a fluid device using the fluid device kit according to any one of claims 1 to 7,
Having a step of inserting the valve into the through-hole,
A method for manufacturing a fluid device. A device body provided with a flow path through which a fluid flows and a hole extending from the flow path,
A flexible valve inserted into the hole,
The valve closes the flow path by being deformed,
The valve is removable from the hole;
Fluid device.

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