JP2019176830A - Magnetic adsorbent recovery tool and genetic testing kit - Google Patents

Abstract

To provide a magnetic adsorbent recovery tool that can efficiently recover a bacterial cell contained in a sample.SOLUTION: A magnetic adsorbent recovery tool (1) comprises: a suction member (10) in which a passage way (11) having a first end part (14) having a suction port (13) and a second end part (15) to which suction means is connected is formed; and a filter (40) which is provided for the whole region of a cross sectional area of a passage way in a direction orthogonal to a flow direction of liquid, in at least a part of passage way in a flow direction of liquid, and captures magnetic adsorbent (5) while allowing liquid flow.SELECTED DRAWING: Figure 3

Description

  The present invention relates to a magnetic adsorbent recovery tool for recovering a magnetic adsorbent that adsorbs infectious disease-causing bacteria in a specimen, and a genetic test kit including the same.

  In recent years, genetic testing has rapidly spread in the field of clinical diagnosis. Genetic testing is the analysis of nucleic acids, chromosomes, and the like to test for the presence or absence of mutations, karyotypes, etc. related to hereditary diseases. As an example of genetic testing, it is determined whether or not a nucleic acid derived from an infectious disease-causing bacterium as a causative bacterium of tuberculosis or other infectious diseases (hereinafter referred to as “target nucleic acid”) exists in a sample collected from a living body. There is an inspection. The inspection process mainly comprises three steps of pretreatment, nucleic acid amplification, and detection.

  There are various methods for the pretreatment process. As an example, the pretreatment process includes a homogenization process, a bacteria collection process, and a bacteria disruption process. In the homogenization step, a homogenization process is performed in which a treatment liquid that lowers the viscosity of the specimen is added to the specimen to homogenize the specimen. In the collection process, a collection process is performed by collecting a homogenized sample by adding a treatment liquid in which an adsorbent capable of adsorbing (collecting) infectious disease-causing bacteria in the sample is dispersed. In the crushing step, the crushing solution is added to the adsorbent from which the infectious disease-causing bacteria are collected and the homogenization treatment is performed by detaching the infectious disease-causing fungus, and the cells in the sample are added. Bacteria crushing treatment is performed to crush (homogenize). The supernatant after this bacterial crushing treatment is used as an inspection intermediate.

  In the nucleic acid amplification step, a mixed reagent containing a primer that can bind to the target nucleic acid and an amplification reagent that amplifies the target nucleic acid is added to the test intermediate prepared in the pretreatment step, and the target nucleic acid is amplified by a nucleic acid amplification reaction. To obtain an amplification product. The amplification reagent contains a fluorescent substance that can bind to the target nucleic acid. In the detection step, the presence or absence of a target nucleic acid derived from an infectious disease-causing fungus in the sample is determined by measuring the fluorescence of a fluorescent substance bound to the target nucleic acid.

  Here, as an adsorbent used in the collection process, a magnetic adsorbent (hereinafter referred to as “magnetic beads”) in which a polymer that adsorbs bacterial cells is coated on the surface of magnetic particles is widely used.

  In the case of using magnetic beads, in the collection process, a treatment liquid in which magnetic beads are dispersed is added to a homogenized specimen to adsorb the bacterial cells on the magnetic beads. Then, the magnet is immersed in a mixed solution of the specimen and the processing liquid, and the magnetic beads adsorbing the bacterial cells are collected by the magnet and used for the bacterial crushing step.

  As a method for recovering using magnetic beads, there is a method disclosed in Patent Document 1. In the method disclosed in Patent Document 1, a sample liquid containing cells (corresponding to the homogenized specimen) is put into a sample container that contains magnetic beads, and the magnetic beads and the sample liquid are stirred with a mixer. Mix while mixing. Next, the magnet covered with the cover is inserted into the sample container, immersed in the sample solution to attach the magnetic beads to the cover, and the magnetic beads adsorbing the cells are collected.

JP 2008-167722 A

  In the method described in Patent Document 1, the magnetic beads and the homogenized specimen are mixed and stirred in a container, and a magnet is immersed in the mixed solution in the container to collect the magnetic beads to which the bacterial cells are attached. , The mixture is left in the container. For this reason, when the microbial cells that are not adsorbed by the magnetic beads are present in the mixed solution remaining in the container, the microbial cells cannot be used for the inspection. This greatly affects the test result when the amount of cells contained in the sample is small.

  This invention is made | formed in view of such a problem, and it aims at providing the magnetic adsorbent collection | recovery tool which can collect | recover the microbial cells contained in a test substance efficiently.

  As a result of various studies, the present inventor has found that the above object can be achieved by the following invention.

  A magnetic adsorbent collection tool according to an aspect of the present invention is a magnetic adsorbent collection tool for collecting a magnetic adsorbent capable of adsorbing the bacterial cells from a liquid containing bacterial cells, and has a suction port. A suction member formed with a passage having one end and a second end to which suction means is connected; and at least part of the passage in the liquid flow direction, orthogonal to the liquid flow direction And a filter that captures the magnetic adsorbent while allowing the liquid to flow.

  In the above magnetic adsorbent collection tool, when a liquid (specimen) containing bacterial cells and in which the magnetic adsorbent is dispersed is sucked from the suction port of the first end, the magnetic adsorbent is captured by the filter, and then the liquid is The magnetic adsorbent passes through the captured filter. Thereby, possibility that the microbial cell in the liquid which is not adsorbed by the magnetic adsorbent will be adsorbed by the magnetic adsorbent increases, and the microbial cell in the liquid which is not adsorbed by the magnetic adsorbent can be reduced. Therefore, according to the magnetic adsorbent collecting tool, the bacterial cells contained in the specimen can be efficiently collected by collecting the magnetic adsorbent.

  In the magnetic adsorbent collecting tool, the filter is disposed in the passage of the suction member at a predetermined interval from the suction port, and the suction member extends from the suction port of the passage to the filter. The portion may be configured to include an accommodating portion for accommodating the magnetic adsorbent.

  In this aspect, the magnetic adsorbent captured by the filter can be constrained in the accommodating portion of the passage, so that the ratio of contact with the liquid magnetic adsorbent passing through the filter can be improved. Therefore, possibility that the microbial cell in a liquid will be adsorb | sucked to a magnetic adsorption body further increases.

  The magnetic adsorbent collection tool may be configured to further include a magnet provided on the suction member so that a magnetic force reaches at least one of the filter and the housing portion.

  In this aspect, after completing the suction of the liquid, the collected magnetic adsorbent can be held in at least one of the position captured by the filter and the accommodating portion. Therefore, the magnetic adsorbent that has adsorbed the bacterial cells can be used for the bacterial crushing step in a collective state, and the possibility that the target nucleic acid is detected in the subsequent steps can be increased.

  In the magnetic adsorbent recovery tool described above, a groove that is recessed toward the downstream side in the liquid flow direction and that opens in a direction perpendicular to the liquid flow direction is formed on an edge surface surrounding the suction port of the passage. May be.

  In this aspect, even if the suction port of the suction member is pressed against the bottom of the container, the suction of the liquid is not hindered, and the liquid stored in the container can be sucked without leaving the bottom. Therefore, possibility that the microbial cell in a liquid will be adsorb | sucked to a magnetic adsorption body further increases.

  In the above magnetic adsorbent recovery tool, a lid mounted on the container in a state where at least the suction port of the suction member is disposed in a container having an opening and the opening is sealed, and the lid is the A check valve provided on the lid so as to allow the flow of gas from the outside to the inside of the container while being attached to the container, while restricting the reverse flow. May be.

  In this aspect, by attaching a lid to a container containing a liquid containing bacterial cells and containing a magnetic adsorbent and sealing the container, the liquid in the container can be prevented from leaking. Furthermore, since a check valve is provided, the pressure inside the container is maintained at the same pressure as the outside even if the liquid inside the container is sealed, and the liquid is sucked in due to a decrease in the pressure inside the container. There is no hindrance. Therefore, the magnetic adsorbent can be recovered safely without diffusing the cells into the atmosphere.

  A genetic test kit according to another aspect of the present invention includes the magnetic adsorbent collection tool, a cylindrical side wall extending in one direction, and a bottom provided at one end of the side wall, A container provided with the opening at the other end of the side wall; and a seal provided on at least one of the container and the suction member; and the suction member includes the lid on the container. In a mounted state, the seal portion extends from the lid through the opening toward the bottom, and the seal portion includes the lid of the suction member and the container in the state where the lid is mounted on the container. Between the bottom portion, the container is interposed over the entire circumference between the outer surface of the suction member and the inner surface of the side wall of the container so as to divide the interior of the container into a plurality of spaces.

  In the genetic test kit described above, the inside of the container is partitioned into a plurality of spaces by interposing a seal portion between the suction member and the inner surface of the side wall of the container in a state where the lid is attached to the container. Further, in a state where the lid is removed and the suction member is removed from the container, the sealed state by the seal portion is released, and each space inside the container communicates.

  If the reagent is arranged in advance in each space inside the container, the operator can mix the reagent arranged in each space in the lower part of the container by simply pulling out the suction member with the opening of the container facing upward.

  In addition, when the suction member that collects the magnetic adsorbent that has adsorbed bacterial cells is inserted into the container again and a lid is attached, the bacterial cells can be added to the mixed reagent and the container can be sealed. . If the whole container is heated in this state, the bacterial cells can be crushed and the nucleic acid can be amplified while the container is sealed. As described above, the operator performs the mixing of the reagents and the amplification of the nucleic acid using the mixed reagents by a very simple operation of simply removing the suction member from the container, collecting the magnetic adsorbent, and returning it to the container again. be able to.

  ADVANTAGE OF THE INVENTION According to this invention, the magnetic adsorbent collection | recovery tool which can collect | recover the microbial cells contained in a sample efficiently can be provided. In addition, according to the present invention, it is possible to provide a genetic test kit capable of simply, safely and reliably performing a reagent mixing operation and a nucleic acid amplification operation using the mixed reagent with a small number of procedures.

FIG. 1 is a front view of a magnetic adsorbent recovery tool according to this embodiment. FIG. 2 is a bottom view of the magnetic adsorbent recovery tool according to the present embodiment. FIG. 3 is a vertical cross-sectional view of a state in which the magnetic adsorbent recovery tool according to the present embodiment is mounted on a sample container. FIG. 4 is a front partial sectional view showing a state in which the magnetic adsorbent recovery tool according to the present embodiment, to which a syringe is connected, is attached to a sample container. FIG. 5 is a front view of the check valve. FIG. 6 is a longitudinal sectional view of the check valve. FIG. 7 is a diagram illustrating the flow direction of the liquid in the passage. FIG. 8 is a longitudinal sectional view of the genetic test kit according to the present embodiment. FIG. 9 is a diagram showing a state where magnets are arranged on the second direction D12 side of the genetic test kit.

  A magnetic adsorbent recovery tool and a genetic test kit according to an embodiment of the present invention will be described with reference to the drawings.

  The magnetic adsorbent collection tool and gene test kit according to this embodiment are used for testing a specimen containing a gene. For example, in a genetic test for determining the presence or absence of a target nucleic acid derived from an infectious disease-causing fungus (bacteria, specific component) as a causative bacterium of tuberculosis or other infectious diseases in a sample collected from a living body, a magnetic adsorbent It is used to collect infectious disease-causing bacteria. Samples collected from a living body include airway samples such as sputum, gastric juice, and bronchoalveolar lavage fluid.

  In this embodiment, a viscous liquid prepared by adding a homogenization treatment liquid to a specimen collected from a living body is used as a specimen for examination. The homogenization treatment liquid is a treatment liquid having a function of reducing the viscosity of a specimen collected from a living body, and examples thereof include a NALC-NaOH mixed solution and a NaOH aqueous solution. NALC (N-acetyl-L-cysteine) has a reducing action, and reduces the viscosity of the specimen by reducing and dissociating the SS bond in the specimen. Moreover, NaOH (sodium hydroxide) sterilizes other than infectious disease-causing bacteria (specific components) mixed in the specimen. A magnetic adsorbent capable of adsorbing infectious disease-causing bacteria is dispersed in the test specimen.

  Hereinafter, after describing the magnetic adsorbent recovery tool, the genetic test kit will be described.

(Configuration of magnetic adsorbent collection tool)
FIG. 1 is a front view of a magnetic adsorbent recovery tool according to the present embodiment, and FIG. 2 is a bottom view thereof. FIG. 3 is a longitudinal sectional view of a state in which the lid of the magnetic adsorbent recovery tool according to the present embodiment is attached to the sample container. FIG. 4 is a front partial cross-sectional view of a state in which the magnetic adsorbent recovery tool according to the present embodiment, to which a syringe is connected, is attached to a sample container. The magnetic adsorbent collection tool 1 includes a suction member 10 in which a passage 11 in which a liquid containing bacterial cells and a magnetic adsorbent (hereinafter also simply referred to as “liquid”) flows is formed, and a filter provided in the passage 11. 40.

(Suction member)
The suction member 10 extends in the axial direction D1, has a tubular shape, and has a passage 11 formed therein. The passage 11 includes a first end 14 having a suction port 13 and a second end 15 to which a syringe 4 (suction means) is connected. In the following description, in the axial direction D1, the direction from the first end portion 14 of the passage 11 toward the second end portion 15 is directed to the first direction D11, and the second end portion 15 is directed to the first end portion 14. The direction is referred to as a second direction D12. The direction in which the liquid sucked by the syringe 4 flows through the passage 11 coincides with the first direction D11.

  The suction member 10 includes a first tubular portion 20, a second tubular portion 30 coupled so that the axis thereof coincides with the first tubular portion 20, and a check valve attached to the lid body 24 of the first tubular portion 20. 17, and a check valve fixing member 16 that fixes the check valve 17 to the lid body 24. The second tubular portion 30 is arranged on the second direction D12 side of the first tubular portion 20.

(First tubular part)
The first tubular portion 20 includes a syringe connecting portion 22 (connecting portion) to which the syringe 4 is detachably connected, a main body 21 extending in the second direction D12 from the end of the syringe connecting portion 22 on the second direction D12 side, The engaged portion 26 provided at the distal end portion of the main body 21 on the second direction D12 side, the lid body 24 provided at a position closer to the first direction D11 than the engaged portion 26 of the main body 21, and the lid body 24 and an external screw 23 provided between the syringe connecting portion 22.

  The syringe connection portion 22 is substantially cylindrical, and the end portion on the first direction D11 side of the syringe connection portion 22 forms the second end portion 15 of the passage 11. The syringe connector 22 has a female luer taper that fits into a male luer taper (not shown) of the syringe 4 with the inner diameter gradually decreasing from the end on the first direction D11 side in the second direction D12. On the outer surface of the end portion on the first direction D11 side of the syringe connecting portion 22, an external screw 22a that is screwed with an internal screw (not shown) provided in the suction port 4a of the syringe 4 is disposed.

  The engaged portion 26 is disposed on the outer surface of the end portion of the main body 21 on the second direction D12 side. The engaged portion 26 protrudes in a direction orthogonal to the axial direction D1 from the outer surface of the main body 21, and constitutes an inverted truncated cone-like claw whose protruding amount increases from the second direction D12 toward the first direction D11. The engaged portion 26 engages with an engaging portion 31 of the second tubular portion 30 described later.

  A magnet holding rib 27 that bulges from the inner surface of the main body 21 toward the passage 11 in a direction orthogonal to the axial direction D1 is disposed at the end of the main body 21 on the second direction D12 side. The magnet holding rib 27 extends from the position on the first direction D11 side to the position exposed from the end on the second direction D12 side of the main body 21 relative to the end on the first direction D11 side of the engaged part 26. .

  The lid 24 includes a disk-shaped top plate 24a that bulges from the outer surface of the main body 21 in a direction orthogonal to the axial direction D1, and a cylindrical side wall 24b that extends from the edge of the top plate 24a toward the second direction D12. Have. An inner screw 24c is provided on the inner surface of the side wall 24b. The inner screw 24 c can be screwed with an outer screw 55 provided in the vicinity of the opening 56 of the sample container 50. The lid 24 is attached to the sample container 50 in a state where at least the suction port 13 of the suction member 10 is disposed inside the sample container 50 and the opening 56 of the sample container 50 is sealed. A through-hole 24d is provided in the top plate 24a, and a check valve 17 that allows inflow of outside air into the specimen container 50 when sucked by the syringe 4 connected to the suction member 10 is provided in the through-hole 24d. It is inserted. In the drawing, specific shapes of the inner screw 24c and the outer screw 55 of the specimen container 50 are omitted.

  FIG. 5 is a front view of the check valve, and FIG. 6 is a longitudinal sectional view of the check valve. The check valve 17 is attached to the through hole 24d of the lid body 24 from the first direction D11 side and is sandwiched between the top plate 24a of the lid body 24 and the check valve fixing member 16 so that the through hole 24d A flange 17a that secures airtightness and a flange 17a that is disposed on the end surface of the flange 17a on the second direction D12 side, closes when the pressure on the second direction D12 side is equal to or higher than the pressure on the first direction D11 side, and pressure on the first direction D11 side Is provided with a valve portion 17b that opens when the pressure is higher than the pressure on the second direction D12 side. The flange 17a has a disk shape with a substantially constant diameter. The valve portion 17b is, in order from the flange 17a side, a cylindrical constant diameter portion having a constant outer diameter and smaller than the outer diameter of the flange 17a, and a truncated cone-shaped enlarged diameter portion whose outer diameter gradually increases from the constant diameter portion side. And a truncated cone-shaped reduced-diameter portion whose outer diameter gradually decreases from the enlarged-diameter portion side. The constant diameter part, the enlarged diameter part, and the reduced diameter part of the flange 17a and the valve part 17b are arranged so that their axes coincide with each other. The outer diameter of the end of the valve portion 17b opposite to the flange 17a is smaller than that of the constant diameter portion.

  The check valve 17 has an air passage 17c penetrating from the flange 17a to the tip of the valve portion 17b in the shaft portion. The air passage 17c has a tapered shape in which the inner diameter gradually decreases from the end portion on the flange 17a side toward the end portion on the distal end side of the valve portion 17b. In a state where the lid body 24 is attached to the specimen container 50 and the check valve 17 is inserted into the through hole 24d of the top plate 24a of the lid body 24 from the first direction D11 side, the pressure inside the specimen container 50 is an external pressure. If it is lower, the check valve 17 opens to allow the gas flow from the outside to the inside of the sample container 50. On the other hand, when the pressure inside the sample container 50 is equal to or higher than the external pressure, the check valve 17 is closed to restrict the flow of gas from the inside of the sample container 50 toward the outside.

  The outer screw 23 is formed on the outer surface of the main body 21, and the inner screw of the check valve fixing member 16 is screwed together. In the drawing, specific shapes of the outer screw 23 and the inner screw of the check valve fixing member 16 are omitted. A through hole 24d is provided in a range overlapping the diameter of the portion of the top plate 24a of the lid 24 where the external screw 23 is provided. A groove in the circumferential direction of the outer screw 23 is recessed in the direction perpendicular to the axial direction D1 and opened in the axial direction D1 in order to open the check valve 17 fitted in the through hole 24d toward the first direction D11. 23a is provided.

  The check valve fixing member 16 has an inner screw and a surface that sandwiches the flange 17a of the check valve 17 with the lid 24 when the inner screw is screwed into the outer screw 23 of the first tubular portion 20. . In a state where the check valve 17 is inserted into the through hole 24d of the lid body 24 from the first direction D11 side and the inner screw of the check valve fixing member 16 is screwed to the outer screw 23, the top plate 24a of the lid body 24 is A part of the flange 17 a of the check valve 17 is sandwiched between the check valve fixing member 16 and the check valve 17 is fixed to the lid body 24. The check valve 17 is bonded to the lid body 24 with an adhesive. In this way, the check valve 17 is fixed and bonded to the lid body 24, thereby ensuring airtightness of the through hole 24 d of the lid body 24.

(Second tubular part)
The second tubular portion 30 is entirely cylindrical, and is disposed on the filter housing portion 32 that houses the filter 40 and the first direction D11 side of the filter housing portion 32, and the engaged portion 26 of the first tubular portion 20. An engaging portion 31 that engages with each other, a magnetic adsorbent accommodating portion 33 (accommodating portion) that is disposed on the second direction D12 side of the filter accommodating portion 32 and accommodates the magnetic adsorbent 5, and the second tubular portion 30 The suction port 13 is provided at the end on the second direction D12 side and sucks liquid. The outer diameter of the second tubular portion 30 is constant from the end on the first direction D11 side to the position between the end on the first direction D11 side and the end on the second direction D12 side. From the position to the end on the second direction D12 side is a tapered shape that gradually decreases in the second direction D12.

  The filter housing portion 32 has a constant inner diameter, and the inner diameter of the filter housing portion 32 is substantially the same as the outer diameter of the end portion on the second direction D12 side of the main body 21 of the first tubular portion 20.

  The engaging portion 31 is configured by a groove formed on the inner surface of the second tubular portion 30 so as to gradually become deeper in the first direction D11 from the second direction D12 side. The engaging part 31 has a shape in which the claw of the engaged part 26 of the first tubular part 20 can be fitted.

  The magnetic adsorbent housing part 33 has a constant inner diameter, which is smaller than the inner diameter of the filter housing part 32. The end of the magnetic adsorbent housing part 33 on the second direction D12 side constitutes the first end 14 of the passage 11 and forms the suction port 13. An edge surface 35 surrounding the suction port 13 of the magnetic adsorbent housing 33 is recessed toward the downstream side in the liquid flow direction, that is, in the first direction D11 and opens in a direction perpendicular to the liquid flow direction. A groove 36 is formed. The length of the magnetic adsorbent accommodating part 33 in the axial direction D1, that is, the distance from the suction port 13 to the bottom 42 of the filter 40 is determined according to the amount of the magnetic adsorbent 5 to be used. It is set to a length that can accommodate all the adsorbents 5.

(filter)
The filter 40 is disposed in the entire region of the cross-sectional area of the passage 11 in a direction orthogonal to the liquid flow direction in at least a part of the passage 11 in the liquid flow direction of the passage 11 of the suction member 10. The filter 40 is formed of a sponge, a porous body, or the like, and captures the magnetic adsorbent 5 while allowing liquid to pass. The filter 40 includes a bottom portion 42 and a cylindrical portion 41 extending from the surface of the bottom portion 42 on the first direction D11 side to the first direction D11 side while being accommodated in the filter accommodation portion 32 of the second tubular portion 30. . The bottom 42 of the filter 40 closes the passage 11 at the end of the magnetic adsorbent housing portion 33 on the first direction D11 side, and the vicinity of the outer edge is in the first direction D11 side of the magnetic adsorbent housing portion 33 of the second tubular portion 30. It touches the end face.

  A cylindrical magnet 45 is disposed on the tubular portion 41 of the filter 40 so as to be in contact with the entire inner surface of the tubular portion 41. In a state where the filter 40 is housed in the filter housing portion 32, the outer surface of the tubular portion 41 of the filter 40 is in contact with the inner surface of the filter housing portion 32 over the entire circumference. That is, in the state where the magnet 45 is accommodated, the tubular portion 41 of the filter 40 is interposed between the outer surface of the magnet 45 and the inner surface of the filter accommodation portion 32 over the entire circumference.

  Further, the thickness (the length in the axial direction D1) of the bottom portion 42 of the filter 40, the length in the axial direction D1 of the magnet 45, and the second holding direction D12 side of the main body 21 of the magnet holding rib 27 of the first tubular portion 20. The sum of the lengths of the portions exposed from the end portions substantially coincides with the length of the filter housing portion 32 in the axial direction D1. Thereby, in the state which connected the 1st tubular part 20 and the 2nd tubular part 30, since the end surface by the side of the 1st direction D11 of the magnet 45 touches the magnet holding rib 27, the magnet 45 in the filter accommodating part 32 is attached. Movement toward the first direction D11 is restricted.

  The liquid that has flowed from the suction port 13 of the suction member 10 by the suction of the syringe 4 flows from the bottom portion 42 of the filter 40 through the cylindrical portion 41 to the first tubular portion 20 side in the passage 11. Further, the magnetic adsorbent 5 in the liquid is captured on the surface of the bottom portion 42 of the filter 40 on the second direction D12 side. FIG. 3 shows a state where the magnetic adsorbent 5 is captured by the filter 40 and accommodated in the magnetic adsorbent accommodating portion 33. The captured magnetic adsorbent 5 is held in the captured position by the magnet 45 even after the liquid has been sucked.

  The magnet 45 preferably has a magnetic pole at each of an end portion on the first direction D11 side and an end portion on the second direction D12 side. 3 is distributed, a relatively strong magnetic field is generated in the bottom portion 42 of the filter 40 and the filter accommodating portion 32 of the suction member 10 which are portions for capturing the magnetic adsorbent 5, and the magnetic force is exerted. Can be made. Therefore, at the time of suction by the syringe 4, the magnetic adsorbent 5 can be reliably gathered by the magnetic force, and the magnetic adsorbent 5 can be reliably held at the captured position.

(Sample container)
In a state where the magnetic adsorbent collecting tool 1 is mounted on the sample container 50, the sample container 50 has a cylindrical side wall 51 extending in the axial direction D1 and a bottom portion provided at the end of the side wall 51 on the second direction D12 side. 52 and a flange 53 that bulges outward from the outer surface in a direction perpendicular to the axial direction D1 at a position between the end portion on the first direction D11 side and the end portion on the second direction D12 side of the side wall 51. Have.

  The side wall 51 includes an opening 56 formed at the end on the first direction D11 side, a constant diameter portion extending from the opening 56 to the flange and having a substantially constant outer diameter, and the flange 53 on the second direction D12 side. Another constant diameter portion extending from the position toward the second direction D12 and having substantially constant outer diameter and inner diameter, and the second direction D12 side of the side wall 51 from the end of the constant diameter portion on the second direction D12 side And a tapered portion that gradually decreases in outer diameter and inner diameter. The inclination of the inner surface of the taper portion is set to be substantially the same as the inclination of the outer surface near the end portion on the second direction D12 side of the second tubular portion 30 constituting the suction member 10 of the magnetic adsorbent collecting tool 1, and The diameter is set slightly larger than the diameter of the suction port 13 of the second tubular portion 30.

  An outer screw 55 that engages with the inner screw 24c of the lid 24 of the suction member 10 is provided on the outer surface of the constant diameter portion of the side wall 51 on the first direction D11 side of the flange 53. Further, the inner diameter of the constant diameter portion is constant from the end on the first direction D11 side to the position on the first direction D11 side of the flange 53 from the first direction D11 side, and from the position to the second direction of the flange. It gradually decreases to the position on the D12 side.

  The length of the specimen container 50 in the axial direction D1 is such that the suction member 10 of the magnetic adsorbent 5 is inserted from the opening 56 side of the specimen container 50 from the suction port 13 side and the lid body 24 is attached to the opening 56. The length is such that the bottom 52 of the sample container 50 and the suction port 13 of the suction member 10 are in contact.

  An O-ring 58 is disposed at the end of the sample container 50 on the first direction D11 side. In a state where the lid 24 and the sample container 50 of the magnetic adsorbent collection tool 1 are coupled, an O-ring 58 is provided between the end of the sample container 50 on the first direction D11 side and the top plate 24a of the lid 24. In a state of being crushed, it is interposed over the entire circumference, and the airtightness of the joint between the sample container 50 and the lid 24 is ensured.

(How to assemble magnetic adsorbent collection tool)
The magnetic adsorbent collection tool 1 according to this embodiment is assembled by the following procedure.

  First, the magnet 45 is inserted into the filter 40, and the filter 40 containing the magnet 45 is inserted into the filter housing portion 32 of the second tubular portion 30. At this time, the vicinity of the outer edge of the bottom portion 42 of the filter 40 is in contact with the end surface on the first direction D11 side of the magnetic adsorbent housing portion 33 of the second tubular portion 30, and the movement of the filter 40 in the second direction D12 side is restricted. The

  Next, the end portion on the second direction D12 side of the first tubular portion 20 is inserted into the engaging portion 31 on the first direction D11 side of the second tubular portion 30. At this time, the edge of the end portion on the first direction D11 side of the engaging portion 31 of the second tubular portion 30 gets over after contacting the engaged portion 26 of the first tubular portion 20. Thus, the end portion on the second direction D12 side of the first tubular portion 20 is accommodated in the engaging portion 31 of the second tubular portion 30, and the engaging portion 31 and the engaged portion 26 are engaged with each other. As a result, the suction member 10 is completed. At this time, the end surface on the second direction D12 side of the first tubular portion 20 is in contact with the end surface on the first direction D11 side of the tubular portion 41 of the filter 40, and the second direction of the magnet holding rib 27 of the first tubular portion 20 is. The end on the D12 side is in contact with the end of the magnet 45 on the first direction D11 side. Thereby, the movement of the filter 40 and the magnet 45 in the filter housing portion 32 toward the first direction D11 is restricted, and the filter 40 and the magnet 45 are stably held inside the filter housing portion 32.

  Next, the valve portion 17b of the check valve 17 is inserted from the first direction D11 side of the through hole 24d of the top plate 24a of the lid body 24, and the check valve fixing member 16 is inserted into the external screw 23 of the first tubular portion 20. Screw the inner screw. Accordingly, the flange 17 a of the check valve 17 is sandwiched between the top plate 24 a of the lid body 24 and the check valve fixing member 16, and the check valve 17 is fixed to the lid body 24.

  The assembly of the magnetic adsorbent collection tool 1 is completed by the above procedure.

(How to use magnetic adsorbent recovery tool)
A method of using the magnetic adsorbent collection tool 1 according to this embodiment will be described.

  First, a test sample is prepared. As a specimen for examination, sputum collected from a living body is used as a viscous liquid prepared using the above-mentioned NALC-NaOH mixed solution as a homogenization treatment solution (homogenization step).

  This viscous liquid is put into the specimen container 50 containing the bacterial collection treatment liquid, and the viscous liquid and the bacterial collection treatment liquid are mixed to obtain a mixed liquid. As the bacteria collection treatment solution, a treatment solution containing a magnetic adsorbent capable of adsorbing infectious disease-causing bacteria is used. Examples of such a bacterial collection treatment solution include a solution containing TB-Beads (registered trademark), which is a magnetic adsorbent (manufactured by Nippon BCG Co., Ltd.).

  The suction member 10 of the magnetic adsorbent collection tool 1 according to this embodiment is inserted from the suction port 13 side through the opening 56 of the specimen container 50 that stores the mixed liquid, and the lid 24 is attached to the specimen container 50. At this time, the sample container 50 and the magnetic adsorbent collection tool 1 are coupled, the opening 56 is sealed by the lid 24, and the inside of the sample container 50 is sealed. Further, the suction port 13 of the suction member 10 of the magnetic adsorbent collection tool 1 is in contact with the bottom 52 of the sample container 50.

  In a state where the sample container 50 and the magnetic adsorbent collecting tool 1 are coupled, the internal screw provided in the suction port 4a of the syringe 4 is screwed into the external screw 22a of the syringe connecting portion 22 of the inhalation member 10, thereby magnetic adsorption. The body recovery tool 1 and the syringe 4 are combined. FIG. 4 shows a state in which the sample container 50, the magnetic adsorbent collection tool 1, and the syringe 4 are combined, and shows a state in which the magnetic adsorbent 5 is dispersed in the mixed liquid in the sample container 50.

  FIG. 7 is a diagram illustrating the flow direction of the liquid in the passage. After the syringe 4 is coupled, the sample container 50 is held so that the second direction D12 side is the lower side in the vertical direction, and when the mixed solution in the sample container 50 is sucked with the syringe 4, the mixing is performed in the direction indicated by the arrow in FIG. Liquid flows. That is, the mixed solution in the sample container 50 flows toward the suction port 13 of the suction member 10, and the mixed solution flowing in from the groove 36 provided around the suction port 13 passes through the passage 11 toward the syringe connecting portion 22. It flows in the first direction D11. At this time, the magnetic adsorbent 5 in the mixed liquid is attracted toward the magnetic adsorbent accommodating portion 33 and the filter 40 by the magnetic force of the magnet 45 in addition to the attractive force of the syringe 4.

  More specifically, the liquid mixture flowing from the suction port 13 of the suction member 10 comes into contact with the bottom 42 of the filter 40 through the magnetic adsorbent housing part 33. At this time, since the bottom portion 42 of the filter 40 blocks the opening at the end of the magnetic adsorbent housing portion 33 on the first direction D11 side, the entire amount of the sucked liquid mixture comes into contact with the filter 40. In the filter 40, since the flow of the mixed solution is allowed, the mixed solution flows in the bottom portion 42 of the filter 40 in a direction orthogonal to the axial direction D1, and in the tubular portion 41 of the filter 40 in the first direction D11. After flowing in the direction orthogonal to the axial direction D1 again at the end on the first direction D11 side of the tubular portion 41, the flow passes through the passage 11 in the first tubular portion 20 toward the first direction D11. It is accommodated in the syringe 4 through the connection part 22.

  Here, since the magnetic adsorbent 5 is captured by the filter 40, all of the magnetic adsorbent 5 in the sucked liquid mixture is captured by the filter 40. Further, the captured magnetic adsorbent 5 is restrained in the magnetic adsorbent accommodating portion 33. Further, the mixed liquid flowing in from the suction port 13 comes into contact with the magnetic adsorbent 5 captured by the filter 40 and restrained in the magnetic adsorbent accommodating portion 33. Therefore, when there is a microbial cell that is not adsorbed by the magnetic adsorbent 5 in the mixed solution, the possibility that the microbial cell is adsorbed by the magnetic adsorbent 5 captured by the filter 40 can be improved. Bacteria in the liquid not adsorbed on the adsorbent 5 can be reduced. By collecting the magnetic adsorbent 5 that has adsorbed the bacterial cells, the bacterial cells contained in the liquid can be efficiently collected.

  After the inhalation of the mixed solution is completed, the lid 24 is removed from the sample container 50, the magnetic adsorbent collecting tool 1 is taken out, and the magnetic adsorbent 5 accommodated in the magnetic adsorbent accommodating portion 33 is subjected to the bacteria crushing step. At this time, since the magnetic adsorbent 5 is held in the magnetic adsorbent accommodating portion 33 by the magnetic force of the magnet 45, the magnetic adsorbent 5 having adsorbed the bacterial cells can be used for the bacterial crushing step in a collective state. The possibility that the target nucleic acid is detected in this step can be increased.

  In the magnetic adsorbent recovery tool 1 according to the present embodiment, since the liquid mixture flows from the groove 36 provided around the suction port 13, mixing is performed even if the tip of the suction member 10 is brought into contact with the bottom 52 of the sample container 50. Liquid suction is not hindered. Thereby, the liquid mixture can be sucked in until the liquid level of the liquid mixture in the specimen container 50 is lowered near the bottom 52, and almost all of the liquid mixture can be sucked up. The possibility of being adsorbed by the magnetic adsorbent 5 captured by 40 can be improved. In the present embodiment, since the side wall 51 of the sample container 50 has a tapered portion, the mixed liquid can be collected at the bottom 52 of the sample container 50, and the mixed liquid in the sample container 50 can be sucked more reliably.

  When the magnetic adsorbent recovery tool 1 according to the present embodiment is used, the liquid mixture in the sample container 50 sealed with the lid 24 is reduced by the suction by the syringe 4, via the check valve 17. Thus, outside air flows from the outside of the sample container 50 into the inside. Therefore, even if the mixed liquid is inhaled, the inhalation of the mixed liquid is not hindered by the decrease in the pressure in the sample container 50. Therefore, the magnetic adsorbent 5 can be safely recovered without diffusing the cells into the atmosphere.

(Modification of magnetic adsorbent recovery tool)
In the above embodiment, the suction member 10 includes the first tubular portion 20 and the second tubular portion 30 which are separate members. However, the first tubular portion 20 and the second tubular portion 30 are integrally formed. May be.

  Further, either or both of the magnetic adsorbent housing part 33 and the magnet 45 can be omitted. In this case, the state in which the magnetic adsorbent 5 is captured is maintained by at least one of the unevenness of the surface of the filter 40, the surface tension of the liquid attached to the filter 40, and the suction force of the syringe 4.

  The container for storing the specimen can be of any size and shape as long as the suction port 13 of the suction member 10 can be inserted, and can be a general-purpose container such as a test tube or a beaker. A dedicated container can be used. The above-described sample container 50 is an example of a dedicated container. When it is not necessary to seal the container as in the case of using a general-purpose container, the lid 24 may be omitted.

  The syringe 4 may be detachable from the inhalation member 10 at the syringe connection portion 22 or may be formed integrally with the inhalation member 10.

(Configuration of genetic test kit)
Next, the genetic test kit according to this embodiment will be described.

  FIG. 8 is a longitudinal sectional view of the genetic test kit according to the present embodiment. The genetic test kit 2 is provided in the magnetic adsorbent collection tool 1, a reagent container 60 (container) for separating and storing a plurality of reagents used in the nucleic acid amplification process, and the suction member 10 of the magnetic adsorbent collection tool 1. And a partition O-ring 90 (seal part) that partitions the space inside the reagent container 60 into a first sealing space 66 and a second sealing space 67. The magnetic adsorbent recovery tool 1 has a flange 25 that bulges from the outer surface of the main body 21 in a direction perpendicular to the axial direction D1 on the first tubular portion 20 of the suction member 10 on the first direction D11 side of the engaged portion 26. In the central portion of the flange 25 in the axial direction D1, a concave portion 25a is provided in the circumferential direction. The compartment O-ring 90 is disposed in the recess 25 a of the flange 25 of the first tubular portion 20.

  Next, the reagent container 60 will be described.

(Reagent container)
In a state where the magnetic adsorbent recovery tool 1 is mounted on the reagent container 60, the reagent container 60 includes a cylindrical side wall 61 extending in the axial direction D1 and a bottom portion provided at the end of the side wall 61 on the second direction D12 side. 62, and an opening 63 is formed at the end of the side wall 61 on the first direction D11 side.

  The reagent container 60 includes a first container member 70 and a second container member 80 that are coupled so that their axes coincide with each other. The second container member 80 is disposed on the first direction D11 side of the first container member 70.

(First container member)
The first container member 70 has a cylindrical first side wall 71 that constitutes a part of the side wall 61 of the reagent container 60, and a bottom 62 that closes the opening of the first side wall 71 on the second direction D12 side. An opening is formed at the end on the one direction D11 side. An external screw 74 is provided at the end of the first side wall 71 on the first direction D11 side.

  An end of the first side wall 71 on the second direction D12 side is formed in a tapered shape that extends toward the bottom 62 and gradually decreases in inner diameter. The inclination of the inner surface of the tapered portion of the first side wall 71 is substantially the same as the inclination of the outer surface in the vicinity of the end portion on the second direction D12 side of the second tubular portion 30 constituting the suction member 10 of the magnetic adsorbent recovery tool 1. The diameter of the bottom 62 is set slightly larger than the diameter of the suction port 13 of the suction member 10.

  The outer screw 74 is screwed with an inner screw 88 of the second container member 80 described later. In the drawing, specific shapes of the outer screw 74 and the inner screw 88 are omitted.

  The material which comprises the 1st container member 70 is not specifically limited, A synthetic resin can be used. As the synthetic resin, for example, polypropylene (PP), polycarbonate (PC), polymethyl methacrylate (PMMA), cycloolefin polymer (COP) and the like can be used. Further, the first container member 70 may be made of a plurality of materials, and preferably includes a light-transmitting portion on at least a part of the first side wall 71 and the bottom portion 62.

(Second container member)
The second container member 80 is disposed on the cylindrical second side wall 81 constituting a part of the side wall 61 of the reagent container 60 and the outer surface of the second side wall 81, and the end of the second side wall 81 on the first direction D11 side. And a flange 86 that bulges outward from the outer surface in a direction orthogonal to the axial direction D1 at a position between the first portion and the end portion on the second direction D12 side.

  The second side wall 81 includes a first opening 83 formed at the end on the first direction D11 side, a second opening formed at the end on the second direction D12 side, and a flange extending from the first opening 83. A constant diameter portion extending to 86 and having a substantially constant outer diameter and inner diameter, a tapered portion extending from the position on the second direction D12 side of the flange 86 toward the second direction D12, and the inner diameter gradually decreasing, and a taper And a constant diameter portion extending from the end portion on the second direction D12 side of the portion to the end portion on the second direction D12 side and having a substantially constant outer diameter and inner diameter. The flange 86 forms a surface whose end on the first direction D11 side is perpendicular to the axial direction D1.

  An outer screw 87 is provided on the outer surface of the constant diameter portion on the first direction D11 side of the flange 86 of the second side wall 81. The outer screw 87 is screwed with the inner screw 24 c of the lid 24 of the magnetic adsorbent collection tool 1. In the drawing, specific shapes of the outer screw 87 and the inner screw 24c are omitted.

  The portion of the second side wall 81 from the end on the second direction D12 side to the position between the end on the second direction D12 side and the end on the first direction D11 side is the opening of the first container member 70. The insertion part 85 to be inserted is configured. The inner surface of the insertion portion 85 is in contact with the compartment O-ring 90 in a state where the suction member 10 of the magnetic adsorbent collection tool 1 is inserted into the reagent container 60 and the lid 24 is mounted.

  The second side wall 81 is provided with a cylindrical threaded portion 82 so as to surround the insertion portion 85 of the second side wall 81. The screwing portion 82 is provided with an inner screw 88 which is screwed with the outer screw 74 of the first container member 70 on the inner surface.

  The second container member 80 can be made of the same material as the first container member 70. Although the 2nd container member 80 does not need to have a light transmittance, it is preferable to have a light transmittance.

  When the outer screw 74 of the first container member 70 and the inner screw 88 of the second container member 80 are screwed together, the first container member 70 and the second container member 80 are coupled to form the reagent container 60. At this time, the insertion portion 85 of the second container member 80 is disposed inside the first side wall 71. Further, the first opening 83 of the second container member 80 constitutes the opening 63 of the reagent container 60.

  A first O-ring 64 is disposed in a groove formed between the screwing portion 82 and the insertion portion 85 of the second container member 80. In a state in which the outer screw 74 of the first container member 70 and the inner screw 88 of the second container member 80 are screwed together, the first O-ring 64 has an entire circumference between the first container member 70 and the second container member 80. Thus, the airtightness of the connecting portion between the first container member 70 and the second container member 80 is ensured.

  A second O-ring 65 is disposed at the end of the reagent container 60 on the first direction D11 side. In a state where the lid 24 of the magnetic adsorbent body recovery tool 1 and the reagent container 60 are coupled, the second O-ring 65 is connected to the end of the second container member 80 on the first direction D11 side and the top plate 24a of the lid 24. The airtightness of the joint portion between the reagent container 60 and the lid body 24 is ensured by being crushed between the entire circumference. For the first O-ring 64 and the second O-ring 65, for example, an elastomer can be used.

  As shown in FIG. 8, the partition O-ring 90 protrudes to the outermost side in the range from the end of the suction member 10 on the second direction D12 side to the lid body 24. In addition, when the suction member 10 is inserted into the reagent container 60 and the lid 24 is attached to the reagent container 60, the suction member 10 extends from the lid 24 toward the bottom 62 through the opening 63. In this state, the partition O-ring 90 first seals the interior of the reagent container 60 at a position between the lid 24 and the end of the suction member 10 on the second direction D12 side (the recess 25a of the flange 25). Between the outer surface of the suction member 10 and the inner surface of the side wall 61 of the reagent container 60 (the inner surface of the insertion portion 85), the entire periphery is interposed so as to partition the space 66 and the second sealing space 67. Further, the space closer to the first direction D11 than the partition O-ring 90 of the reagent container 60, that is, the second sealing space 67 surrounded by the second container member 80, is the suction member 10, the lid 24, the partition O-ring 90. The second O-ring 65 is sealed.

  In the reagent container 60, the reagent container 60 is held such that the bottom 62 is on the lower side in the vertical direction, and the reagent container 60 is disposed in the second sealed space 67 with a predetermined amount of reagent disposed in the first sealed space 66. A predetermined amount of reagent is mixed on the bottom 62 side of the reagent container 60. In this state, when the suction member 10 is inserted into the reagent container 60 and the lid 24 is attached to the reagent container 60, the liquid level of the mixed reagent is the same as the surface of the bottom 42 of the filter 40 on the second direction D12 side. The dimension of the reagent container 60 and the amount of the reagent are set so that the position is closer to the first direction D11 than that.

(Modification of genetic test kit)
In the above embodiment, the suction member 10 and the partition O-ring 90 are separate members, but may be formed integrally. Further, the partition O-ring 90 may be provided in the second container member 80 instead of being provided in the first tubular portion 20 of the suction member 10. In this case, the flange 25 of the first tubular portion 20 can be omitted, and the partition O-ring 90 can be provided on the inner peripheral surface of the second container member 80. Further, the second container member 80 and the compartment O-ring 90 may be integrally formed. When the compartment O-ring 90 is provided on the inner peripheral surface of the second container member 80, the second container member 80 and the compartment O-ring 90 are arranged so that the compartment O-ring 90 protrudes inwardly on the inner surface of the side wall 61 of the reagent container 60. Need to form.

  The first container member 70 and the second container member 80 constituting the reagent container 60 may be integrally formed. At this time, the first O-ring 64 and the screwing portion 82 of the second container member 80 are unnecessary.

(Reagent placement method)
A method for arranging reagents in the first sealed space 66 and the second sealed space 67 of the genetic test kit 2 according to the present embodiment will be described.

  First, a predetermined amount of the first reagent is injected into the first container member 70 before being combined with the second container member 80. As the first reagent, for example, an amplification reagent that is a reagent that causes a nucleic acid amplification reaction can be used. The amplification reagent contains an enzyme that amplifies the target nucleic acid and a fluorescent substance that binds to the target nucleic acid.

  Next, the insertion portion 85 of the second container member 80 is inserted from the opening of the first container member 70, and the external screw 74 of the first container member 70 and the internal screw 88 of the second container member 80 are screwed together. . Thereby, the first container member 70 and the second container member 80 are combined to form the reagent container 60.

  A first O-ring 64 is disposed in a groove formed between the insertion portion 85 and the screwing portion 82 of the second container member 80 before being coupled to the first container member 70. Since the 2nd container member 80 is provided with the screwing part 82, the 1st container member 70 and the 2nd container member 80 can be combined reliably.

  Subsequently, the second O-ring 65 is disposed at the end of the second container member 80 of the reagent container 60 on the first direction D11 side, and the suction member 10 is opened from the suction port 13 side to the opening 63 of the reagent container 60, that is, the second container. The lid body 24 is attached to the reagent container 60 by being inserted into the first opening 83 of the member 80 and screwing the inner screw 24 c of the lid body 24 and the outer screw 87 of the reagent container 60. At this time, the suction member 10 is in a state in which the check valve 17 and the check valve fixing member 16 are not attached to the lid body 24. In the present embodiment, the first opening 83 of the second container member 80 and the constant diameter portion of the second side wall 81 subsequent thereto have a relatively large inner diameter, and the tip of the suction member 10 is smoothly second due to the tapered portion. Since it is guided to the direction D12 side, the insertion of the suction member 10 is easy.

  Through the above steps, the first reagent is disposed in the first sealed space 66, the first sealed space 66 is sealed, and the second reagent can be disposed in the second sealed space 67.

  Next, a predetermined amount of the second reagent is injected into the second sealing space 67 from the through hole 24 d of the top plate 24 a of the lid 24. As the second reagent, for example, a primer can be used. The primer is a reagent for a nucleic acid amplification reaction with respect to a target nucleic acid derived from an infectious disease-causing bacterium.

  After injecting the second reagent into the second sealing space 67, the check valve 17 is inserted into the through hole 24d from the first direction D11 side, and the inner screw of the check valve fixing member 16 is screwed into the outer screw 23. .

  In this manner, predetermined amounts of the first reagent and the second reagent are separately disposed and sealed in the first sealed space 66 and the second sealed space 67, respectively. Even if the genetic test kit 2 vibrates during transportation, the first reagent and the second reagent are held in a state where they are separated into the first sealed space 66 and the second sealed space 67.

(Gene detection method using genetic test kit)
About the gene detection method using the genetic test kit according to the present embodiment in which a predetermined amount of the first reagent and the second reagent are arranged in the first sealed space 66 and the second sealed space 67, respectively, and sealed. explain.

  First, a specimen for examination is prepared, and a viscous liquid is prepared using a homogenized treatment liquid as described in the above-mentioned “How to Use Magnetic Adsorbent Recovery Tool” (homogenization step). Is put in a specimen container 50 containing a bacterial collection treatment solution, and the viscous liquid and the bacterial collection treatment solution are mixed to obtain a mixed solution. At this time, any sample container may be used.

  Next, the genetic test kit 2 according to the present embodiment is held so that the lid 24 is at the top, the lid 24 is opened, and the inhalation member 10 is taken out from the reagent container 60. At this time, in the reagent container 60, the first sealing space 66 and the second sealing space 67 communicate with each other, and the second reagent is removed from the second sealing space 67 by the insertion portion 85 of the second container member 80. A mixed reagent in which the first reagent and the second reagent are mixed at a predetermined ratio is obtained by being guided to the one sealed space 66 side. The second reagent is smoothly guided to the first sealed space 66 side without staying along the inner surface of the tapered portion of the second side wall 81.

  The first reagent is an amplification reagent and the second reagent is a primer. The amplification reagent includes a mixed solution of HNB and GelGreen as a labeling fluorescent substance.

  Then, as described in the above “How to Use Magnetic Adsorbent Recovery Tool”, the sample container 50 and the magnetic adsorbent recovery tool 1 are combined, the magnetic adsorbent recovery tool 1 and the syringe 4 are combined, and the syringe In step 4, the mixed liquid in the sample container 50 is sucked to collect the magnetic adsorbent (bacteria collection step).

  The suction member 10 containing the magnetic adsorbent in the magnetic adsorbent containing portion 33 is inserted into the reagent container 60 again, and the lid 24 is closed. Thereby, the genetic test kit 2 is sealed in a state where the magnetic adsorbent is immersed in the mixed reagent inside the reagent container 60.

  The sealed genetic test kit 2 is sufficiently vibrated to mix the mixed reagent and the magnetic adsorbent.

  Subsequently, the genetic test kit 2 is heated at about 68 ° C. for 30 to 70 minutes. Heating is performed using, for example, a heat block. Due to the heating, the infectious disease-causing bacteria are crushed into pieces of cells due to cell membrane degradation or the like (bacteria crushing step). A target nucleic acid derived from an infectious disease-causing fungus dispersed in a mixed reagent by crushing is amplified by a nucleic acid amplification reaction (nucleic acid amplification step). In this embodiment, after mixing the amplification reagent and the primer, since the bacteria disruption step and the nucleic acid amplification step can be performed quickly, the effectiveness of the mixed reagent can be sufficiently exerted, and the presence or absence of the target nucleic acid is high. Can be judged with accuracy.

  FIG. 9 is a diagram showing a state where magnets are arranged on the second direction D12 side of the genetic test kit. When the genetic test kit 2 is heated, the magnet 6 that exerts a magnetic field stronger than the magnet 45 in the filter 40 on the filter 40 or the magnetic adsorbent housing part 33 may be disposed on the second direction D12 side of the genetic test kit 2. The magnet 6 can pull out the magnetic adsorbent 5 accommodated in the magnetic adsorbent accommodating portion 33 to the bottom 62 of the reagent container 60 so that the magnetic adsorbent 5 can be easily touched to the mixed reagent. Thereby, reaction of a mixed reagent can be accelerated and test | inspection time can be shortened.

  After heating, the reagent in the gene test kit 2 is observed under excitation light, the presence or absence of fluorescence is determined, the presence or absence of the target nucleic acid is determined, and the test is completed.

  If it is determined that there is fluorescence under excitation light, the sample is determined to be positive, that is, the target nucleic acid is present, and if it is determined that there is no fluorescence, it is determined to be negative, that is, the target nucleic acid is not present.

  As described above, in the genetic test kit 2 according to the present embodiment, a predetermined amount of the first reagent and the second reagent are arranged in the first sealed space 66 and the second sealed space 67 in advance, respectively. The member 10 is removed from the reagent container 60, and the magnetic adsorbent is recovered from the mixed liquid containing the sample and returned to the reagent container 60 again, so that a highly accurate test can be performed.

  For this reason, for example, an examination can be performed even when a specialized worker is absent, such as at night, and an appropriate response can be taken for the patient.

  The determination of the presence or absence of fluorescence when observing the genetic test kit 2 under excitation light may be made visually or using a fluorescence detector. In the present embodiment, since the reagent container 60 has a tapered shape in which the inner diameter becomes smaller as it is closer to the bottom portion 62, the density of the target nucleic acid is increased in the vicinity of the bottom portion 62, the fluorescence emission is increased, and the determination accuracy is increased.

  When the first container member 70 includes a light-transmitting part on at least a part of the first side wall 71 and the bottom part 62, the first container member 70 transmits fluorescence through the light-transmitting part without removing the lid 24 from the reagent container 60. The presence or absence can be determined. Therefore, after the lid 24 is mounted on the reagent container 60, the infection-causing bacteria can be crushed, nucleic acid amplification and detection can be performed with the reagent container 60 sealed. There is little risk of scattering in the air, and the risk of contamination and the infection risk of workers can be greatly reduced.

  When the second container member 80 and the lid 24 are also light transmissive, the presence / absence of fluorescence can be determined even from the lid 24 side, and the convenience of the inspection operator is further improved.

  Commercially available heating devices and fluorescence detectors can be used. Therefore, if these devices are already owned, the genetic test kit of this embodiment can be used without newly making capital investment.

1 Magnetic Adsorbent Collection Tool 2 Genetic Testing Kit 4 Syringe (Suction Unit)
5 Magnetic Adsorbent 10 Suction Member 11 Passage 13 Suction Port 14 First End 15 Second End 17 Check Valve 24 Lid 33 Magnetic Adsorbent Storage (Storage)
35 Edge surface surrounding suction port 36 Groove 40 Filter 45 Magnet 60 Reagent container (container)
61 Side wall 62 Bottom 90 Compartment O-ring (seal part)

Claims (6)

A magnetic adsorbent recovery tool for recovering a magnetic adsorbent capable of adsorbing the bacterial cells from a liquid containing bacterial cells,
A suction member formed with a passage having a first end having a suction port and a second end to which a suction means is connected;
At least part of the passage in the liquid flow direction is provided in the entire area of the cross-sectional area of the passage in a direction orthogonal to the liquid flow direction, and captures the magnetic adsorbent while allowing the liquid flow. And a magnetic adsorbent collection tool. The filter is disposed at a predetermined interval from the suction port in the passage of the suction member,
2. The magnetic adsorbent collection tool according to claim 1, wherein the suction member includes an accommodating portion that accommodates the magnetic adsorbent in a portion of the passage from the suction port to the filter.   The magnetic adsorbent collection tool according to claim 1, further comprising a magnet provided on the suction member so that a magnetic force reaches at least one of the filter and the storage portion.   The surface of the edge surrounding the suction port of the passage has a groove that is recessed toward the downstream side in the liquid flow direction and opens in a direction perpendicular to the liquid flow direction. The magnetic adsorbent collection tool according to 1. A lid mounted on the container in a state where at least the suction port of the suction member is disposed in a container having an opening and the opening is sealed;
With the lid mounted on the container, a check valve provided on the lid so as to allow a gas flow from the outside to the inside of the container while restricting a reverse flow; The magnetic adsorbent collection tool according to any one of claims 1 to 4, further comprising: A magnetic adsorbent recovery tool according to claim 5;
The container having a cylindrical side wall extending in one direction and a bottom provided at one end of the side wall, and the opening formed at the other end of the side wall;
A seal portion provided on at least one of the container and the suction member,
The suction member extends from the lid toward the bottom through the opening with the lid attached to the container,
The seal portion is configured to divide the inside of the container into a plurality of spaces between the lid of the suction member and the bottom of the container in a state where the lid is attached to the container. A genetic test kit interposed between the outer surface of the member and the inner surface of the side wall of the container over the entire circumference.

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