CN116640647A - Kit and nucleic acid detection system - Google Patents
Kit and nucleic acid detection system Download PDFInfo
- Publication number
- CN116640647A CN116640647A CN202310637813.0A CN202310637813A CN116640647A CN 116640647 A CN116640647 A CN 116640647A CN 202310637813 A CN202310637813 A CN 202310637813A CN 116640647 A CN116640647 A CN 116640647A
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- China
- Prior art keywords
- valve
- valve shaft
- communicated
- extraction tank
- shaft assembly
- Prior art date
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- 238000001514 detection method Methods 0.000 title claims abstract description 20
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 17
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 17
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 17
- 239000007788 liquid Substances 0.000 claims abstract description 154
- 238000000605 extraction Methods 0.000 claims abstract description 130
- 210000001503 joint Anatomy 0.000 claims abstract description 32
- 230000009471 action Effects 0.000 claims abstract description 9
- 238000007789 sealing Methods 0.000 claims description 46
- 239000010408 film Substances 0.000 claims description 15
- 239000013039 cover film Substances 0.000 claims description 7
- 238000003032 molecular docking Methods 0.000 claims description 6
- 238000004891 communication Methods 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims 2
- 238000003752 polymerase chain reaction Methods 0.000 description 10
- 238000010586 diagram Methods 0.000 description 6
- 239000000243 solution Substances 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 2
- 239000000443 aerosol Substances 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000005086 pumping Methods 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/54—Labware with identification means
- B01L3/545—Labware with identification means for laboratory containers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/56—Labware specially adapted for transferring fluids
- B01L3/567—Valves, taps or stop-cocks
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
A kit and a nucleic acid detection system relate to the technical field of nucleic acid detection. The kit comprises a valve body, an extraction tank arranged below the valve body, an air nozzle, a reaction tube and a valve shaft assembly; one end of the valve shaft component penetrates through the valve body, the other end of the valve shaft component extends into the extraction tank, the valve shaft component is rotationally connected with the valve body and the extraction tank, the valve body is provided with a plurality of liquid storage cavities which are not communicated with each other and are arranged around the rotating shaft of the valve shaft component, and the valve shaft component is provided with a butt joint flow channel communicated with the extraction tank; one end of the air nozzle is communicated with the interior of the extraction tank, and the other end of the air nozzle is connected with the vacuum pump; the valve shaft assembly is driven to rotate so that the butt joint flow channel is communicated with any liquid storage cavity or a reaction tube; the butt joint flow channel is communicated with the liquid storage cavity, so that liquid in the corresponding liquid storage cavity can flow into the extraction tank; the butt-joint flow channel is communicated with the reaction tube, so that the liquid in the extraction tank can be pressed into the reaction tube under the action of the vacuum pump. The kit and the nucleic acid detection system have simple structures and can provide continuous and stable pressure to control the flow of liquid in the kit.
Description
Technical Field
The application relates to the technical field of nucleic acid detection, in particular to a kit and a nucleic acid detection system.
Background
PCR (Polymerase Chain Reaction ) technology is a molecular biological technique that amplifies specific DNA (deoxyribonucleic acid ) sequences in vitro. The PCR technology has the characteristics of strong specificity, high sensitivity, low purity requirement, simplicity, convenience and rapidness, and is widely applied to molecular biological detection and analysis. Conventional nucleic acid detection needs to be performed in a PCR laboratory, and according to the requirements of regulations, the PCR laboratory needs to be partitioned according to a reagent preparation area, a nucleic acid extraction area, an amplification area and a detection area, and all relevant experimenters need to have PCR on-duty certificates. Therefore, the molecular biological detection and analysis in the PCR laboratory has high requirements for experimental operation environment and personnel experimental quality, and even if the requirements are met, the accuracy of the detection result may be affected by the pollution of the aerosol.
In order to solve the influence of aerosol pollution in the external environment on the detection result, a kit is currently presented, and the kit transfers samples, reagents and the like by using a plurality of on-off valves in a mode of internally arranging a plurality of liquid storage cavities. However, the existing kit mostly adopts a piston rod internally arranged to control the flow direction of liquid, so that the structure is complex, the piston stroke is easily limited, the volume of the positive and negative pressure gas is limited, and the flow of liquid is limited and cannot be complemented for a long time.
Disclosure of Invention
The application aims to provide a kit and a nucleic acid detection system, which have simple structures and can provide continuous and stable pressure to control the flow of liquid in the kit.
Embodiments of the present application are implemented as follows:
in one aspect of the application, a kit is provided, which comprises a valve body, an extraction tank arranged below the valve body, an air nozzle, a reaction tube and a valve shaft assembly; one end of the valve shaft component penetrates through the valve body, the other end of the valve shaft component extends into the extraction tank, the valve shaft component is rotationally connected with the valve body and the extraction tank, the valve body is provided with a plurality of liquid storage cavities which are not communicated with each other, the liquid storage cavities are arranged around the rotating shaft of the valve shaft component, and the valve shaft component is provided with a butt joint flow channel communicated with the extraction tank; one end of the air nozzle is communicated with the interior of the extraction tank, and the other end of the air nozzle is connected with a vacuum pump; the valve shaft assembly is driven to rotate so that one end of the butt joint flow channel far away from the extraction tank is communicated with any one liquid storage cavity or a reaction tube; the butt joint flow channel is communicated with the liquid storage cavity, so that liquid in the corresponding liquid storage cavity can flow into the extraction tank; the butt-joint runner is communicated with the reaction tube, so that liquid in the extraction tank can be pressed into the reaction tube under the action of the vacuum pump. The kit and the nucleic acid detection system are simple in structure and can provide continuous and stable pressure to control the flow of liquid in the kit.
Optionally, the kit further comprises a valve seat, the valve seat being located between the valve body and the extraction well; the valve seat is provided with a first through hole, a plurality of second through holes arranged around the first through hole and an extraction flow channel; the reaction tube is connected to the valve seat and is communicated with the extraction flow channel; one end of the valve shaft assembly, which is far away from the extraction tank, sequentially passes through the first through holes and the valve body, and the plurality of second through holes are in one-to-one correspondence with the plurality of liquid storage cavities; the valve shaft assembly is driven to rotate, and the butt joint runner can be communicated with the corresponding liquid storage cavity through the second through hole, or the butt joint runner can be communicated with the reaction tube through the extraction runner.
Alternatively, the reaction tubes comprise two, and the valve shaft assembly is driven to rotate so that the butt-joint flow channel is communicated with the two reaction tubes.
Optionally, the valve body includes first body, and first body has the third through-hole that supplies the valve shaft subassembly to pass, and a plurality of stock solutions chamber link up first body and encircle setting up in the periphery of third through-hole.
Optionally, the valve body further comprises a bottom sealing film positioned between the first body and the valve seat, and the bottom sealing film is used for sealing the bottom of the liquid storage cavity; a plurality of puncture parts are convexly arranged on one surface of the valve seat, which faces the back cover film, and a plurality of second through holes extend towards the back cover film and penetrate through the puncture parts; when the valve body is arranged on the valve seat, the puncture part can penetrate through the bottom sealing film to be communicated with the liquid storage cavity.
Optionally, the kit further comprises a valve cover arranged above the valve body, wherein the valve cover is used for blocking the inlet end of the liquid storage cavity.
Optionally, the valve shaft assembly comprises a valve shaft and a valve core connected with the valve shaft, a first flanging is arranged at one end of the valve shaft, which is close to the valve core, and a second flanging fixedly connected with the first flanging is arranged at one end of the valve core, which is close to the first flanging; the first flange is provided with a through butt joint hole, the second flange is provided with a first liquid channel, the valve core is provided with a second liquid channel, and the butt joint hole, the first liquid channel and the second liquid channel are sequentially communicated to form a butt joint flow channel.
Optionally, the first flange and the valve shaft are integrally formed, the second flange and the valve core are integrally formed, and the first flange and the second flange are welded and connected.
Optionally, a sealing ring is sleeved on the valve shaft, the valve seat is in sealing connection with the first flanging through the sealing ring, and the sealing ring is provided with a fourth through hole which is communicated with the second through holes in one-to-one correspondence and a fifth through hole which is communicated with the extraction flow passage.
In another aspect of the present application, there is provided a nucleic acid detection system comprising the kit described above.
The beneficial effects of the application include:
the kit provided by the application comprises a valve body, an extraction tank, an air nozzle, a reaction tube and a valve shaft assembly, wherein the extraction tank, the air nozzle, the reaction tube and the valve shaft assembly are arranged below the valve body; one end of the valve shaft component penetrates through the valve body, the other end of the valve shaft component extends into the extraction tank, the valve shaft component is rotationally connected with the valve body and the extraction tank, the valve body is provided with a plurality of liquid storage cavities which are not communicated with each other, the liquid storage cavities are arranged around the rotating shaft of the valve shaft component, and the valve shaft component is provided with a butt joint flow channel communicated with the extraction tank; one end of the air nozzle is communicated with the interior of the extraction tank, and the other end of the air nozzle is connected with a vacuum pump; the valve shaft assembly is driven to rotate so that one end of the butt joint flow channel far away from the extraction tank is communicated with any one liquid storage cavity or a reaction tube; the butt joint flow channel is communicated with the liquid storage cavity, so that liquid in the corresponding liquid storage cavity can flow into the extraction tank; the butt-joint runner is communicated with the reaction tube, so that liquid in the extraction tank can be pressed into the reaction tube under the action of the vacuum pump. When the kit works, the valve shaft assembly is driven to rotate relative to the valve body, and the butt-joint flow channel on the valve shaft assembly can be selectively communicated with any liquid storage cavity, so that liquid in the liquid storage cavity flows into the extraction tank through the butt-joint flow channel; when liquid in the extraction tank is required to enter the reaction tube, the valve shaft assembly is rotated, the butt-joint flow channel of the valve shaft assembly is communicated with the reaction tube, the vacuum pump charges air into the extraction tank through the air nozzle, and at the moment, the liquid in the extraction tank can be pressed into the reaction tube through the butt-joint flow channel under the action of air pressure, so that polymerase chain reaction is carried out in the reaction tube. The kit provided by the application has a simple structure, and the flow direction of liquid is controlled by using a 6-way mode that the vacuum pump pumps and aerates the air nozzle, so that compared with the control of the flow direction of the liquid by adopting the piston rod, the pressure of the kit is not limited by the stroke of the piston, stable and continuous pressure can be provided, the pressure can be compensated for a long time, and the control effect on the flow direction of the liquid is better; the extraction tank is independent of the liquid storage cavity of the valve body, can accommodate liquid with larger volume, and has good application prospect.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic structural diagram of a kit according to an embodiment of the present application;
FIG. 2 is a schematic diagram of a valve cover and a valve body according to an embodiment of the present application;
FIG. 3 is a second schematic view of a valve cover and a valve body according to an embodiment of the present application;
fig. 4 is a schematic structural diagram of a first body of a valve body according to an embodiment of the present application;
FIG. 5 is a schematic illustration of one of the valve shaft assembly, valve seat and extraction cell structures provided in an embodiment of the present application;
FIG. 6 is a second schematic illustration of a valve shaft assembly, valve seat and extraction cell provided in accordance with an embodiment of the present application;
FIG. 7 is a schematic view of a valve seat and seal ring according to an embodiment of the present application;
FIG. 8 is a schematic diagram of a valve seat according to an embodiment of the present application;
FIG. 9 is a schematic illustration of one of the valve shaft assembly and extraction cell structures provided in an embodiment of the present application;
FIG. 10 is a second schematic illustration of a valve shaft assembly and extraction cell provided in accordance with an embodiment of the present application;
FIG. 11 is a schematic diagram of an extraction tank according to an embodiment of the present application;
fig. 12 is a second schematic diagram of the structure of the extraction tank according to the embodiment of the present application.
Icon: 10-a valve body; 11-a liquid storage cavity; 12-a first body; 121-a third through hole; 13-a back cover film; 14-capping film; 15-capping; 16-a bottom sealing piece; 20-an extraction tank; 21-a first gap; 22-a first channel; 23-a second gap; 24-a first cover plate; 25-a first groove; 26-a second groove; 30-air nozzle; 31-a filter element; 40-reaction tube; 50-a valve shaft assembly; 51-butting flow channels; 52-a valve shaft; 53-a first flanging; 531-butt holes; 54-valve core; 541-a second liquid channel; 55-second flanging; 551-a first liquid channel; 56-sealing rings; 561-fourth through holes; 562-fifth through holes; 60-valve seat; 61-a first through hole; 62-a second through hole; 63-extracting the flow channel; 631-extraction wells; 632-extraction path; 64-piercing section; 65-a second cover plate; 70-valve cover.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present application more apparent, the technical solutions of the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application, and it is apparent that the described embodiments are some embodiments of the present application, but not all embodiments of the present application. The components of the embodiments of the present application generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the application, as presented in the figures, is not intended to limit the scope of the application, as claimed, but is merely representative of selected embodiments of the application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
In the description of the present application, it should be noted that, directions or positional relationships indicated by terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., are directions or positional relationships based on those shown in the drawings, or are directions or positional relationships conventionally put in use of the inventive product, are merely for convenience of describing the present application and simplifying the description, and are not indicative or implying that the apparatus or element to be referred to must have a specific direction, be constructed and operated in a specific direction, and thus should not be construed as limiting the present application. Furthermore, the terms "first," "second," "third," and the like are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.
Furthermore, the terms "horizontal," "vertical," and the like do not denote a requirement that the component be absolutely horizontal or overhang, but rather may be slightly inclined. As "horizontal" merely means that its direction is more horizontal than "vertical", and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.
In the description of the present application, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present application will be understood in specific cases by those of ordinary skill in the art.
Referring to fig. 1 to 6, the present embodiment provides a kit, which includes a valve body 10, an extraction tank 20 disposed below the valve body 10, an air nozzle 30, a reaction tube 40, and a valve shaft assembly 50; one end of the valve shaft assembly 50 penetrates through the valve body 10 and the other end of the valve shaft assembly 50 extends into the extraction tank 20, the valve shaft assembly 50 is rotationally connected with the valve body 10 and the extraction tank 20, the valve body 10 is provided with a plurality of liquid storage cavities 11 which are not communicated with each other, the liquid storage cavities 11 are arranged around the rotating shaft of the valve shaft assembly 50, and the valve shaft assembly 50 is provided with a butt joint flow passage 51 communicated with the extraction tank 20; one end of the air nozzle 30 is communicated with the interior of the extraction tank 20, and the other end is connected with a vacuum pump; the valve shaft assembly 50 is driven to rotate so that one end of the butt joint flow channel 51 far away from the extraction tank 20 is communicated with any one of the liquid storage cavities 11 or the reaction tube 40; the butt-joint flow channel 51 is communicated with the liquid storage cavity 11, so that liquid in the corresponding liquid storage cavity 11 can flow into the extraction tank 20; the abutting flow passage 51 is communicated with the reaction tube 40, and can enable liquid in the extraction tank 20 to be pressed into the reaction tube 40 under the action of a vacuum pump. The kit and the nucleic acid detection system are simple in structure and can provide continuous and stable pressure to control the flow of liquid in the kit.
In this embodiment, referring to fig. 2, 3 and 4, the valve body 10 is provided with a plurality of liquid storage cavities 11 that are not communicated with each other, and fig. 4 is an example of ten liquid storage cavities 11, and it should be understood that ten liquid storage cavities 11 are only examples given by the present application and are not limiting to the specific number of liquid storage cavities 11, and those skilled in the art can select a suitable number of liquid storage cavities 11 according to the need. It should be noted, however, that no matter how many liquid storage chambers 11 are provided, two adjacent liquid storage chambers 11 should be isolated from each other.
In the present embodiment, the plurality of liquid storage chambers 11 are disposed around the rotation axis of the valve shaft assembly 50, so that the abutting flow passages 51 on the valve shaft assembly 50 can communicate with different liquid storage chambers 11 when the valve shaft assembly 50 is rotated.
The extraction tank 20 is located below the valve body 10, and when the liquid storage chamber 11 is communicated with the extraction tank 20 through the butt-joint flow channel 51 of the valve shaft assembly 50, the liquid in the liquid storage chamber 11 can enter the extraction tank 20, so that an amplification reaction occurs in the extraction tank 20.
One end of the air nozzle 30 is communicated with the interior of the extraction tank 20, and the other end of the air nozzle is communicated with a vacuum pump, so that the kit provided by the application can be inflated into the air nozzle 30 through the vacuum pump, and the liquid in the extraction tank 20 is pressed into the reaction tube 40 through the butt-joint flow channel 51; alternatively, the air nozzle 30 is pumped by a vacuum pump, so that the liquid in the liquid storage cavity 11 is pumped into the extraction tank 20 through the butt-joint flow channel 51.
Referring to fig. 11 and 12 again, the extraction tank 20 is provided with a first notch 21, a first groove 25, a first channel 22, a second groove 26 and a second notch 23 which are sequentially communicated, wherein the air nozzle 30 is communicated with the first notch 21, so that the air nozzle 30 can be inflated by a vacuum pump, so that the gas sequentially passes through the first notch 21, the first groove 25, the first channel 22, the second groove 26 and the second notch 23 to enter the extraction tank 20, and the liquid in the extraction tank 20 is pressed into the reaction tube 40 through the butt joint flow channel 51; or, the air in the extraction tank 20 is pumped out through the second notch 23, the second groove 26, the first channel 22, the first groove 25 and the first notch 21 in sequence by pumping the air in the air nozzle 30 through the vacuum pump, so that the liquid in the liquid storage cavity 11 is pumped into the extraction tank 20 after passing through the butt joint flow channel 51.
In order to prevent the liquid in the extraction tank 20 from being sucked into the vacuum pump when the vacuum pump sucks, the application is also provided with a filter element 31 at the air nozzle 30, as shown in fig. 11.
It should be noted that, when the vacuum pump draws the gas in the extraction tank 20 through the air tap 30, the arrangement of the second groove 26 (the depth of the second groove 26 is greater than the depth of the second notch 23) can serve as a first safety for preventing the liquid from being sucked into the vacuum pump, the arrangement of the first passage 22 (the first passage 22 is provided around the outer periphery of the extraction tank 20) can serve as a second safety for preventing the liquid from being sucked into the vacuum pump, the arrangement of the first groove 25 (the depth of the first groove 25 is greater than the depth of the first notch 21) can serve as a third safety for preventing the liquid from being sucked into the vacuum pump, and the arrangement of the filter element 31 can serve as a fourth safety for preventing the liquid from being sucked into the vacuum pump. According to the application, the risk that liquid is pumped into the vacuum pump during air pumping of the vacuum pump can be effectively improved through the four-place arrangement.
In addition, as shown in fig. 11, the present application further includes a first cover plate 24 covering the extraction tank 20, and a through hole is formed in the first cover plate 24, and the through hole is used for the valve shaft assembly 50 (specifically, a valve core 54 of the valve shaft assembly 50, which will be described in detail below) to pass through. The provision of the first cover plate 24 can improve the sealing performance of the gas.
One end of the valve shaft assembly 50 penetrates the valve body 10 and the other end protrudes into the interior of the extraction tank 20, as shown in fig. 1 and 4. In addition, in the present embodiment, the valve shaft assembly 50 can rotate relative to the valve body 10 and the extraction tank 20, so that the docking flow channel 51 on the valve shaft assembly 50 can selectively communicate with different liquid storage chambers 11 or with the reaction tube 40. In this embodiment, the reaction tube 40 is a PCR tube.
In summary, the kit provided by the application includes a valve body 10, an extraction tank 20 disposed below the valve body 10, an air tap 30, a reaction tube 40, and a valve shaft assembly 50; one end of the valve shaft assembly 50 penetrates through the valve body 10 and the other end of the valve shaft assembly 50 extends into the extraction tank 20, the valve shaft assembly 50 is rotationally connected with the valve body 10 and the extraction tank 20, the valve body 10 is provided with a plurality of liquid storage cavities 11 which are not communicated with each other, the liquid storage cavities 11 are arranged around the rotating shaft of the valve shaft assembly 50, and the valve shaft assembly 50 is provided with a butt joint flow passage 51 communicated with the extraction tank 20; one end of the air nozzle 30 is communicated with the interior of the extraction tank 20, and the other end is connected with a vacuum pump; the valve shaft assembly 50 is driven to rotate so that one end of the butt joint flow channel 51 far away from the extraction tank 20 is communicated with any one of the liquid storage cavities 11 or the reaction tube 40; the butt-joint flow channel 51 is communicated with the liquid storage cavity 11, so that liquid in the corresponding liquid storage cavity 11 can flow into the extraction tank 20; the abutting flow passage 51 is communicated with the reaction tube 40, and can enable liquid in the extraction tank 20 to be pressed into the reaction tube 40 under the action of a vacuum pump. When the kit works, the valve shaft assembly 50 is driven to rotate relative to the valve body 10, and the butt-joint flow channel 51 on the valve shaft assembly 50 can be selectively communicated with any one liquid storage cavity 11, so that liquid in the liquid storage cavity 11 flows into the extraction tank 20 through the butt-joint flow channel 51; when the liquid in the extraction tank 20 is required to enter the reaction tube 40, the valve shaft assembly 50 is rotated, so that the butt-joint flow channel 51 of the valve shaft assembly 50 is communicated with the reaction tube 40, the vacuum pump charges air into the extraction tank 20 through the air nozzle 30, and at the moment, the liquid in the extraction tank 20 can be pressed into the reaction tube 40 through the butt-joint flow channel 51 under the action of air pressure, so that the polymerase chain reaction is performed in the reaction tube 40. The kit provided by the application has a simple structure, and the liquid flow direction is controlled by using a mode that the vacuum pump pumps and aerates the air nozzle 30, so that compared with the mode that the liquid flow direction is controlled by adopting the piston rod, the pressure of the kit is not limited by the piston stroke, stable and continuous pressure can be provided, long-time pressure compensation can be realized, and the control effect on the liquid flow direction is better; the extraction tank 20 is independent of the liquid storage cavity 11 of the valve body 10, can accommodate larger volume of liquid, and has better application prospect.
Referring again to fig. 5 and 6, optionally, the kit further comprises a valve seat 60, the valve seat 60 being located between the valve body 10 and the extraction well 20; the valve seat 60 is provided with a first through hole 61, a plurality of second through holes 62 provided around the first through hole 61, and an extraction flow passage 63; the reaction tube 40 is connected to the valve seat 60 and communicates with the extraction flow path 63; one end of the valve shaft assembly 50, which is far away from the extraction tank 20, sequentially passes through the first through hole 61 and the valve body 10, and the plurality of second through holes 62 are in one-to-one correspondence with the plurality of liquid storage cavities 11; the valve shaft assembly 50 is driven to rotate, and the docking flow passage 51 can be communicated with the corresponding liquid storage cavity 11 through the second through hole 62, or the docking flow passage 51 can be communicated with the reaction tube 40 through the extraction flow passage 63.
The valve seat 60 has a first through hole 61 through which the valve shaft assembly 50 can pass, and a plurality of second through holes 62 are in one-to-one correspondence with the plurality of liquid storage cavities 11. The valve shaft assembly 50 is driven to rotate, and the abutting flow passage 51 on the valve shaft assembly 50 can be communicated with the extracting flow passage 63 or any of the second through holes 62. When the butt-joint flow passage 51 of the valve shaft assembly 50 is communicated with any one of the second through holes 62, the liquid in the liquid storage cavity 11 can flow into the extraction tank 20 through the second through holes 62 and the butt-joint flow passage 51 in sequence; when the abutting flow passage 51 and the extracting flow passage 63 of the valve shaft assembly 50 are communicated, the liquid in the extracting tank 20 can sequentially enter the reaction tube 40 through the abutting flow passage 51 and the extracting flow passage 63 under the action of the vacuum pump and the air nozzle 30.
In the present embodiment, as shown in fig. 7 and 8, the extraction flow path 63 includes an extraction hole 631 and an extraction channel 632 that are in communication with each other, wherein the extraction channel 632 is in communication with the reaction tube 40, and the extraction hole 631 is provided in the movement path of the abutting flow path 51 of the valve shaft assembly 50. Thus, by rotating the valve shaft assembly 50 to a proper angle, the docking flow path 51 can communicate with the extraction hole 631 and thus with the reaction tube 40 through the extraction channel 632.
Referring to fig. 8, the valve seat 60 is further provided with a second cover plate 65, and the second cover plate 65 is used for covering the extraction channel 632, so that no liquid overflows when the liquid enters the reaction tube 40 through the extraction channel 632. Of course, the extraction channel 632 may be disposed inside the valve seat 60 instead of on the surface of the valve seat 60, so that the second cover plate 65 is not required.
In this embodiment, alternatively, the reaction tubes 40 may be provided in two, and the valve shaft assembly 50 may be driven to rotate so that the docking flow channel 51 communicates with the two reaction tubes 40.
In one case, when the reaction tubes 40 include two extraction flow paths 63, the two extraction flow paths 63 are connected to the two reaction tubes 40 in a one-to-one correspondence, and thus, when it is necessary to make the liquid enter the reaction tube 40, the butt flow path 51 of the valve shaft assembly 50 and the extraction flow path 63 corresponding to the reaction tube 40 are communicated.
In another case, when the reaction tube 40 includes two extraction holes 631 of the extraction flow path 63, two extraction channels 632, and two extraction channels 632 are connected to the extraction holes 631, the valve shaft assembly 50 is rotated such that the butt flow path 51 of the valve shaft assembly 50 is connected to the extraction holes 631, and then the liquid is introduced into the two reaction tubes 40.
In the above two cases, a person skilled in the art may select an appropriate one according to actual needs, and the present application is not limited thereto.
As shown in fig. 4, the valve body 10 may alternatively include a first body 12, where the first body 12 has a third through hole 121 through which the valve shaft assembly 50 passes, and a plurality of liquid storage chambers 11 penetrate the first body 12 and are disposed around the outer periphery of the third through hole 121.
The shape and volume of each liquid storage cavity 11 are not limited in the application, and can be set by one skilled in the art. Illustratively, the shape of each reservoir 11 may be the same or different, and the volume of each reservoir 11 may be equal or different.
Referring to fig. 3, optionally, the valve body 10 further includes a bottom sealing film 13 located between the first body 12 and the valve seat 60, where the bottom sealing film 13 is used to seal the bottom of the liquid storage cavity 11; the valve seat 60 is provided with a plurality of puncture parts 64 protruding toward one surface of the back cover film 13, and a plurality of second through holes 62 extend toward the back cover film 13 and penetrate the puncture parts 64; when the valve body 10 is mounted on the valve seat 60, the puncture portion 64 can communicate with the liquid storage chamber 11 through the back cover 13.
The bottom sealing film 13 may be used to seal the bottom of the liquid storage cavity 11, so that when in use, liquid may be stored in the liquid storage cavity 11 in advance, and after the liquid in the liquid storage cavity 11 is completely stored, the valve body 10 is mounted on the valve seat 60.
The puncture part 64 arranged on the valve seat 60 can puncture the bottom sealing film 13 at the bottom of the valve body 10 when the valve body 10 is arranged on the valve seat 60, so that the liquid in the liquid storage cavity 11 can be communicated with the second through hole 62 on the valve seat 60 through the puncture part 64, and the preparation is made for the liquid to enter the extraction tank 20 through the butt joint flow channel 51.
Optionally, the kit further comprises a valve cover 70 disposed above the valve body 10, the valve cover 70 being used to block the inlet end of the liquid storage chamber 11. The valve cover 70 can effectively prevent the liquid in the liquid storage cavity 11 from flowing out from the top of the liquid storage cavity 11.
With continued reference to fig. 3, the valve body 10 provided by the present application further includes a top sealing film 14, a top sealing member 15, and a bottom sealing member 16. Wherein, the top sealing member 15 is arranged between the top sealing film 14 and the top of the liquid storage cavity 11, and the top sealing member 15 has an embedded part embedded at the top of the liquid storage cavity 11, so that the embedded part can play a role in improving the outflow of the liquid in the liquid storage cavity 11 from the outer wall of the top to a certain extent. The top sealing film 14 is disposed between the valve cover 70 and the top sealing member 15, and is used for sealing the top outlet of the liquid storage cavity 11. The application can effectively improve the leakage of liquid from the top of the liquid storage cavity 11 through the arrangement of the top sealing film 14, the top sealing piece 15 and the valve cover 70. The bottom sealing member 16 may also have an embedded portion embedded at the bottom of the liquid storage cavity 11, and the functions and arrangement manners of the bottom sealing film 13 and the bottom sealing member 16 are the same as those of the top sealing film 14 and the top sealing member 15, which may be referred to the foregoing description, and for avoiding repeated description, no further description is provided herein.
Referring to fig. 9 and 10 in combination, optionally, the valve shaft assembly 50 includes a valve shaft 52 and a valve core 54 connected to the valve shaft 52, wherein a first flange 53 is disposed at an end of the valve shaft 52 adjacent to the valve core 54, and a second flange 55 fixedly connected to the first flange 53 is disposed at an end of the valve core 54 adjacent to the first flange 53; the first flange 53 is provided with a through butt joint hole 531, the second flange 55 is provided with a first liquid channel 551, the valve core 54 is provided with a second liquid channel 541, and the butt joint hole 531, the first liquid channel 551 and the second liquid channel 541 are sequentially communicated to form the butt joint flow channel 51.
The valve shaft 52 sequentially passes through the first through hole 61 of the valve seat 60 and the third through hole 121 of the valve body 10, and the valve core 54 is located below the valve seat 60 and extends into the extraction tank 20.
The above-mentioned abutting hole 531 on the first flange 53, the first liquid channel 551 on the second flange 55, and the second liquid channel 541 on the valve core 54 are sequentially communicated to form the abutting flow passage 51.
Alternatively, the first flange 53 and the valve shaft 52 are integrally formed, the second flange 55 and the valve core 54 are integrally formed, and the first flange 53 and the second flange 55 are welded.
The first flange 53 may be a flange of the valve shaft 52, and the second flange 55 may be a flange of the valve core 54.
Referring to fig. 7, optionally, a sealing ring 56 is sleeved on the valve shaft 52, the valve seat 60 is connected with the first flange 53 in a sealing manner through the sealing ring 56, and the sealing ring 56 is provided with a fourth through hole 561 in one-to-one correspondence with the plurality of second through holes 62 and a fifth through hole 562 in communication with the extraction flow channel 63.
That is, the sealing ring 56 is sleeved on the valve shaft 52 and is located between the first flange 53 and the valve seat 60, and the sealing ring 56 is provided with a fifth through hole 562 and a plurality of fourth through holes 561. Among them, the fourth through hole 561 and the second through hole 62 communicate, and the fifth through hole 562 and the extraction flow path 63 communicate (specifically, the extraction holes 631 of the extraction flow path 63 communicate).
The ends of the fourth and fifth through-holes 561, 562 facing away from the valve seat 60 may selectively communicate with the abutment channel 51 of the valve shaft assembly 50, and in this embodiment, particularly with the abutment hole 531 of the abutment channel 51.
In another aspect of the present application, there is provided a nucleic acid detection system comprising the kit described above. The specific structure and the beneficial effects of the kit are described in detail in the foregoing, so the description of the application is not repeated here.
It should be noted that, the nucleic acid detecting system may further include a driving motor for driving the valve shaft assembly 50 to rotate, an ultrasonic probe for promoting cell lysis, etc. besides the kit, and the present application is not limited thereto, and a person skilled in the art may set the system according to the need of nucleic acid detection.
The above description is only of alternative embodiments of the present application and is not intended to limit the present application, and various modifications and variations will be apparent to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.
In addition, the specific features described in the above embodiments may be combined in any suitable manner, and in order to avoid unnecessary repetition, various possible combinations are not described further.
Claims (10)
1. The kit is characterized by comprising a valve body, an extraction tank, an air nozzle, a reaction tube and a valve shaft assembly, wherein the extraction tank, the air nozzle, the reaction tube and the valve shaft assembly are arranged below the valve body; one end of the valve shaft assembly penetrates through the valve body, the other end of the valve shaft assembly extends into the extraction tank, the valve shaft assembly is in rotary connection with the valve body and the extraction tank, the valve body is provided with a plurality of liquid storage cavities which are not communicated with each other, the liquid storage cavities are arranged around the rotary shaft of the valve shaft assembly, and the valve shaft assembly is provided with a butt joint flow channel communicated with the extraction tank; one end of the air nozzle is communicated with the interior of the extraction tank, and the other end of the air nozzle is connected with a vacuum pump;
the valve shaft assembly is driven to rotate so that one end, far away from the extraction tank, of the butt joint flow channel is communicated with any one of the liquid storage cavities or the reaction tube; the butt joint flow channel is communicated with the liquid storage cavity, so that liquid in the liquid storage cavity can flow into the extraction tank; the butt-joint runner is communicated with the reaction tube, so that liquid in the extraction tank can be pressed into the reaction tube under the action of the vacuum pump.
2. The kit of claim 1, further comprising a valve seat between the valve body and the extraction well; the valve seat is provided with a first through hole, a plurality of second through holes arranged around the first through hole and an extraction flow channel; the reaction tube is connected to the valve seat and is communicated with the extraction flow channel; one end, far away from the extraction tank, of the valve shaft assembly sequentially passes through the first through holes and the valve body, and the plurality of second through holes are in one-to-one correspondence with the plurality of liquid storage cavities; the valve shaft assembly is driven to rotate, and the butt joint runner can be communicated with the liquid storage cavity corresponding to the second through hole or the butt joint runner can be communicated with the reaction tube through the extraction runner.
3. The kit of claim 1 or 2, wherein the reaction tubes comprise two, and wherein the valve shaft assembly is rotatably driven to enable the docking flow channel to communicate with the two reaction tubes.
4. The kit of claim 2, wherein the valve body comprises a first body having a third through hole through which the valve shaft assembly passes, and wherein the plurality of liquid storage chambers penetrate the first body and are circumferentially disposed around the third through hole.
5. The kit of claim 4, wherein the valve body further comprises a bottom sealing membrane between the first body and the valve seat, the bottom sealing membrane for sealing off the bottom of the liquid storage cavity; a plurality of puncture parts are convexly arranged on one surface of the valve seat, which faces the back cover film, and a plurality of second through holes extend towards the back cover film and penetrate through the puncture parts; when the valve body is mounted on the valve seat, the puncture part can penetrate through the bottom sealing film to be communicated with the liquid storage cavity.
6. The kit of claim 1 or 2, further comprising a valve cover disposed over the valve body for sealing off an inlet end of the reservoir.
7. The kit of claim 2, wherein the valve shaft assembly comprises a valve shaft and a valve core connected with the valve shaft, a first flange is arranged at one end of the valve shaft close to the valve core, and a second flange fixedly connected with the first flange is arranged at one end of the valve core close to the first flange; the first flanging is provided with a through butt joint hole, the second flanging is provided with a first liquid channel, the valve core is provided with a second liquid channel, and the butt joint hole, the first liquid channel and the second liquid channel are sequentially communicated to form the butt joint flow channel.
8. The kit of claim 7, wherein the first flange and the valve shaft are integrally formed, the second flange and the valve cartridge are integrally formed, and the first flange and the second flange are welded together.
9. The kit of claim 7, wherein a sealing ring is sleeved on the valve shaft, the valve seat is in sealing connection with the first flange through the sealing ring, and the sealing ring is provided with a fourth through hole in one-to-one correspondence with the second through holes and a fifth through hole in communication with the extraction flow channel.
10. A nucleic acid detection system, characterized in that ss comprises a kit according to any one of claims 1 to 9.
Priority Applications (1)
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CN202310637813.0A CN116640647A (en) | 2023-05-31 | 2023-05-31 | Kit and nucleic acid detection system |
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CN202310637813.0A CN116640647A (en) | 2023-05-31 | 2023-05-31 | Kit and nucleic acid detection system |
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