JP2019151609A - Composition for promoting growth of plants and use thereof - Google Patents
Composition for promoting growth of plants and use thereof Download PDFInfo
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- JP2019151609A JP2019151609A JP2018039988A JP2018039988A JP2019151609A JP 2019151609 A JP2019151609 A JP 2019151609A JP 2018039988 A JP2018039988 A JP 2018039988A JP 2018039988 A JP2018039988 A JP 2018039988A JP 2019151609 A JP2019151609 A JP 2019151609A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
Abstract
Description
本発明は、植物の成長促進用組成物およびその利用に関する。 The present invention relates to a composition for promoting plant growth and use thereof.
非特許文献１には、Talaromyces（タラロマイセス）属のカビを用いてコマツナの生育を促進する方法が記載されている。 Non-Patent Document 1 describes a method for promoting the growth of Komatsuna using molds of the genus Talaromyces.
しかし、この方法のみでは、植物の生長を促進させる方法に求められる産業界のニーズを全て満たすことはできず、新たな、植物の成長を促進する方法が求められている。 However, this method alone does not satisfy all the industrial needs required for a method for promoting plant growth, and a new method for promoting plant growth is required.
そこで、本発明の目的は、新たな、植物の成長促進用組成物を提供することにある。 Therefore, an object of the present invention is to provide a new composition for promoting plant growth.
本発明者らは、前記目的を達成すべく鋭意研究を重ねた。その結果、特定の耐熱性カビが植物の成長を促進する効果を有することを見出した。この知見は、従来、殺菌することが困難であり、植物を加工して食品などとして利用する際に障害となっていた耐熱性カビについて、成長の促進に利用しようという驚くべきものである。このような本発明者らによる新たな知見によって本発明を完成するに至った。 The inventors of the present invention have intensively studied to achieve the above object. As a result, it was found that specific heat-resistant molds have the effect of promoting plant growth. This finding is surprising that heat-resistant molds that have been difficult to sterilize in the past and have been an obstacle when processing plants and using them as foods are used to promote growth. The present invention has been completed by such new knowledge by the present inventors.
すなわち、本発明の一態様は、
（１）Geminibasidium（ジェミニバシディウム）属の耐熱性カビを含む、ハクサイ、キュウリ、エンドウ、イチゴからなる群から選ばれる植物の成長促進用組成物、
（２）前記Geminibasidium（ジェミニバシディウム）属の耐熱性カビは、Geminibasidium donsium（ジェミニバシディウム ドンシウム）である、（１）の植物の成長促進用組成物、
（３）賦形剤をさらに含む、（１）または（２）の植物の成長促進用組成物、
（４）植物を生育させるための培地に、請求項（１）から（３）のいずれか１項に記載の植物の成長促進用組成物を供給する供給工程を含む、植物の成長促進方法、
（５）Geminibasidium属の耐熱性カビを製剤化する製剤化工程を含む、植物の成長促進用組成物の製造方法、
（６）前記製剤化工程は、５０℃以上、７５℃以下の熱処理工程を含む、（５）の植物の成長促進用組成物の製造方法、
（７）Geminibasidium（ジェミニバシディウム）属の耐熱性カビが定着したハクサイ、キュウリ、エンドウ、イチゴからなる群から選ばれる植物、
である。
That is, one embodiment of the present invention is
(1) A composition for promoting the growth of a plant selected from the group consisting of Chinese cabbage, cucumber, pea, and strawberry, comprising a heat-resistant mold belonging to the genus Geminibasidium.
(2) The heat-resistant mold belonging to the genus Geminibasidium (Geminibasidium) is Geminibasidium donsium (Geminibasidium donsium), (1) the plant growth promoting composition,
(3) The composition for promoting plant growth according to (1) or (2), further comprising an excipient,
(4) A plant growth promoting method comprising a supplying step of supplying the plant growth promoting composition according to any one of claims (1) to (3) to a medium for growing the plant,
(5) A method for producing a composition for promoting plant growth, comprising a formulation step of formulating a heat-resistant mold of the genus Geminibasidium,
(6) The method for producing a plant growth promoting composition according to (5), wherein the formulation step includes a heat treatment step of 50 ° C or higher and 75 ° C or lower,
(7) A plant selected from the group consisting of Chinese cabbage, cucumber, pea, and strawberry, to which heat-resistant molds of the genus Geminibasidium are established,
It is.
本発明によれば、植物の成長を促進させる、新たな植物の成長促進用組成物、及び植物の成長促進方法を提供できる。従って、植物の収穫量の更なる拡大が期待できる。 ADVANTAGE OF THE INVENTION According to this invention, the plant growth promotion composition and the plant growth promotion method which promote the growth of a plant can be provided. Therefore, further expansion of plant yield can be expected.
以下、本発明を詳細に説明する。なお、本発明において、「％」は「質量％」を、「部」は「質量部」を意味する。 Hereinafter, the present invention will be described in detail. In the present invention, “%” means “mass%”, and “part” means “part by mass”.
＜本発明の植物の成長促進用組成物の特徴＞
本発明に係る植物の成長促進用組成物は、Geminibasidium（ジェミニバシディウム）属の耐熱性カビを含む。以下、植物の成長促進用組成物を、単に「組成物」ともいう。本発明に係る組成物は、耐熱性カビが植物の根圏の栄養素を植物の吸収できる形へと変換することで、植物の成長を促進させる効果を奏する。例えば、これらの耐熱性カビが、アミノ体窒素を硝酸体窒素に変換することで、植物体が当該硝酸体窒素を吸収できる。なお、本発明における「成長促進」とは、植物体の乾燥重量、植物体の高さ、植物体の葉数、植物体の葉長、又は植物体の葉幅が、本発明に係る組成物を用いない場合に比べて増加することを意味する。
<Features of Composition for Promoting Plant Growth of the Present Invention>
The composition for promoting plant growth according to the present invention comprises a heat-resistant mold belonging to the genus Geminibasidium. Hereinafter, the composition for promoting plant growth is also simply referred to as “composition”. The composition according to the present invention has an effect of promoting the growth of plants by converting the heat-resistant fungi into nutrients that can be absorbed by the plants. For example, these heat-resistant molds convert amino nitrogen into nitrate nitrogen, so that the plant can absorb the nitrate nitrogen. In the present invention, the term “growth promotion” means that the dry weight of the plant, the height of the plant, the number of leaves of the plant, the leaf length of the plant, or the leaf width of the plant is the composition according to the present invention. It means that it increases compared to the case where no is used.
＜耐熱性カビ＞
本発明の一実施形態に係る組成物に含まれる耐熱性カビは、Geminibasidium（ジェミニバシディウム）属の耐熱性カビである。Geminibasidium（ジェミニバシディウム）属の耐熱性カビとしては、Geminibasidium donsium（ジェミニバシディウム ドンシウム）が好ましい。Geminibasidium donsium（ジェミニバシディウム ドンシウム）を用いることで、特に高い成長促進効果を得ることができる。
<Heat resistant mold>
The heat-resistant mold contained in the composition according to one embodiment of the present invention is a heat-resistant mold belonging to the genus Geminibasidium. As the heat-resistant mold belonging to the genus Geminibasidium, Geminibasidium donsium is preferable. By using Geminibasidium donsium, it is possible to obtain a particularly high growth promoting effect.
耐熱性カビは、土壌等から単離してもよく、寄託機関から取得してもよい。例えば、Geminibasidium donsium（ジェミニバシディウム ドンシウム）として、受託番号ＮＩＴＥ ＢＰ−０２３９５のGeminibasidium donsium（ジェミニバシディウム ドンシウム）ＢＦＦ４１０等を利用することができる。 The heat-resistant mold may be isolated from soil or the like, or obtained from a depository organization. For example, Geminibasidium donsium (Geminibasidium donsium) BFF410 etc. having a deposit number of NITE BP-02395 can be used as Geminibasidium donsium.
本発明の一態様に係る組成物は、以上に例示した耐熱性カビを単独で含んでもよく、複数の種類を含んでもよい。本発明の一態様に係る組成物は、植物の成長を促進する効果を損ねない範囲で、以上に例示した耐熱性カビ以外のカビを含んでもよい。例えば、Leohumicola（レオフミコラ）属、Devriesia shelburniensis（デブリエシア シェルバーニエンシス）等の耐熱性カビ等が挙げられる。耐熱性カビが、Leohumicola（レオフミコラ）属であれば、エンドウ、又はイチゴの成長を特に好適に促進することができる。耐熱性カビが、Devriesia shelburniensis（デブリエシア シェルバーニエンシス）であれば、ハクサイ、トマト、エンドウ、又はイチゴの成長を特に好適に促進することができる。Leohumicola（レオフミコラ）属の中でも、Leohumicola verrucosa（レオフミコラ ヴェルコサ）がより好ましく、Leohumicola verrucosa（レオフミコラ ヴェルコサ）ＢＦＦ３５２（受託番号：ＮＩＴＥ ＢＰ−０２４６７）等を用いればよい。Devriesia shelburniensis（デブリエシア シェルバーニエンシス）としては、Devriesia shelburniensis（デブリエシア シェルバーニエンシス）ＢＦＦ３１３（受託番号：ＮＩＴＥ ＢＰ−０２３９４）等を用いればよい。 The composition according to one embodiment of the present invention may include the heat-resistant molds exemplified above alone or may include a plurality of types. The composition according to one embodiment of the present invention may include molds other than the heat-resistant molds exemplified above as long as the effect of promoting plant growth is not impaired. Examples thereof include heat-resistant molds such as Leohumicola genus and Devriesia shelburniensis. If the heat-resistant mold is in the genus Leohumicola, the growth of peas or strawberries can be promoted particularly suitably. If the heat-resistant mold is Devriesia shelburniensis, the growth of Chinese cabbage, tomato, pea, or strawberry can be particularly preferably promoted. Among the genus Leohumicola, Leohumicola verrucosa is more preferable, and Leohumicola verrucosa BFF352 (accession number: NITE BP-02467) may be used. Devriesia shelburniensis (Devriesia shelveniensis) BFF313 (accession number: NITE BP-02394) or the like may be used.
耐熱性カビの耐熱性は、７５℃、１分間の処理で殺菌できる程度であることが好ましい。食品加工の工程では、病原性大腸菌など衛生細菌の殺滅のため、７５℃、１分間の殺菌が行われている。耐熱性カビの耐熱性が前記の範囲内であれば、この殺菌処理により、耐熱性カビを選抜することができるためである。 The heat resistance of the heat-resistant mold is preferably such that it can be sterilized by treatment at 75 ° C. for 1 minute. In the process of food processing, sterilization is performed at 75 ° C. for 1 minute in order to kill sanitary bacteria such as pathogenic E. coli. If the heat resistance of the heat resistant mold is within the above range, the heat resistant mold can be selected by this sterilization treatment.
＜耐熱性カビの含有量＞
本発明の一実施形態に係る組成物中の耐熱性カビの含有量は、とくに限定されるものではないが、好ましくは、組成物１ｇ当たり、１００個以上１億個以下である。耐熱性カビの配合量が前記の範囲内であることにより、耐熱性カビがより確実に植物に接触及び定着する。
<Content of heat-resistant mold>
The content of heat-resistant mold in the composition according to one embodiment of the present invention is not particularly limited, but is preferably 100 or more and 100 million or less per 1 g of the composition. When the amount of the heat-resistant mold is within the above range, the heat-resistant mold more reliably contacts and settles the plant.
＜耐熱性カビの状態＞
耐熱性カビは、生活環のどの形態で組成物中に含まれていてもよい。例えば菌糸体の状態であってもよく、胞子体の状態であってもよい。なお、耐熱性カビを、後述する製剤化工程において熱処理工程により処理する場合、耐熱性カビが熱により死滅しないよう、耐熱性カビは、胞子の状態であることが好ましい。
<State of heat-resistant mold>
The heat-resistant mold may be contained in the composition in any form of the life cycle. For example, it may be in a mycelium state or a spore state. In addition, when heat-resistant mold is processed by the heat treatment step in the formulation step described later, the heat-resistant mold is preferably in a spore state so that the heat-resistant mold is not killed by heat.
＜植物＞
本発明の一実施形態に係る組成物は様々な植物に適用することができ、対象となる植物は特に限定されない。例えば、一般的に食用に供される植物の栽培に用いることができる。一般的に栽培が難しいとされるブルーベリーの栽培にも用いることが可能である。本実施形態に係る組成物により特に好適に成長を促進される植物は、ハクサイ、キュウリ、エンドウ、またはイチゴである。中でも、イチゴの成長を特に好適に促進することができる。
<Plant>
The composition which concerns on one Embodiment of this invention can be applied to various plants, and the plant used as object is not specifically limited. For example, it can be used for cultivation of plants generally used for food. It can also be used for cultivation of blueberries, which are generally considered difficult to grow. Plants whose growth is particularly preferably promoted by the composition according to the present embodiment are Chinese cabbage, cucumber, pea, or strawberry. Especially, the growth of strawberry can be promoted especially suitably.
本発明の一実施形態に係る植物の成長促進用組成物、及び植物の成長促進方法は、様々な成長段階の植物に適用することができる。例えば、植物が種であるときに組成物を培地に供給してもよく、植物が苗木、樹木であるときに供給してもよい。 The composition for promoting plant growth and the method for promoting plant growth according to one embodiment of the present invention can be applied to plants in various growth stages. For example, the composition may be supplied to the medium when the plant is a seed, or may be supplied when the plant is a seedling or a tree.
本発明の一実施形態に係る組成物を用いて生育した植物は、本発明に係る組成物を用いずに生育した植物と全く同様に利用できる。また、本発明の一実施形態に係る組成物を用いて生育した植物からは、アフラトキシン、パツリン、デオキシニバレノール、及びオクラトキシンＡ等のカビ毒が、人体に有害な量検出されない。 A plant grown using the composition according to one embodiment of the present invention can be used in exactly the same manner as a plant grown without using the composition according to the present invention. In addition, mold poisons such as aflatoxin, patulin, deoxynivalenol, and ochratoxin A are not detected from plants grown using the composition according to one embodiment of the present invention.
＜その他の成分＞
本発明の一実施形態に係る組成物は、耐熱性カビに加えて、その他の成分を含んでいてもよい。その他の成分としては例えば、賦形剤、安定剤、界面活性剤、展着剤等の添加剤が挙げられる。安定剤とは耐熱性カビの胞子又は菌糸が長期間生存できるようにするものであり、例えば特開２００４−３５４２１、特開２０１０−１４３８５６に記載の安定剤等が挙げられる。界面活性剤は耐熱性カビが植物に浸透し易くするためのものであり、従来公知の界面活性剤が挙げられる。展着剤は植物に浸透した後の耐熱性カビが植物に付着しやすくするためのものであり、例えば界面活性剤、多糖類を使用でき、界面活性剤としては、特開２００５−７５８１３、特開２００８−３１０８３、特開２０１１−１８４３７０、特開２０１２−７２１１６、国際公開公報第２０１２０６３８２４号、特開２０１６−１３５１０１等で用いられている界面活性剤が挙げられ、多糖類としては、でんぷん、キサンタンガム、グアーガム、ローストビーンガム、カラギーナン等が挙げられる。
<Other ingredients>
The composition according to one embodiment of the present invention may contain other components in addition to the heat-resistant mold. Examples of other components include additives such as excipients, stabilizers, surfactants, and spreading agents. Stabilizers are those that allow heat-resistant mold spores or mycelia to survive for a long period of time, and examples include stabilizers described in JP-A Nos. 2004-35421 and 2010-143856. Surfactants are used for making heat-resistant molds easily penetrate plants, and include conventionally known surfactants. The spreading agent is for making the heat-resistant mold after infiltrating the plant easy to adhere to the plant. For example, surfactants and polysaccharides can be used. Examples include surfactants used in KAI 2008-31083, JP-A 2011-184370, JP-A 2012-72116, International Publication No. 20122063824, JP-A-2006-135101, and the like. , Guar gum, roast bean gum, carrageenan and the like.
好ましくは、本発明の一実施形態に係る植物の成長促進用組成物は、その他の成分として、賦形剤、又は安定剤を含んでいる。植物の成長促進用組成物が、賦形剤を含んでいることにより、植物の成長促進用組成物の取扱い、及び成形を容易にすることができる。植物の成長促進用組成物が、安定剤を含んでいることにより、品質劣化を防ぐことができる。 Preferably, the composition for promoting plant growth according to an embodiment of the present invention contains an excipient or a stabilizer as the other component. When the composition for promoting plant growth contains an excipient, handling and shaping of the composition for promoting plant growth can be facilitated. Since the composition for promoting plant growth contains a stabilizer, quality deterioration can be prevented.
＜組成物の形状＞
本発明の一実施形態に係る植物の成長促進用組成物の形状は、特に限定されるものではなく、様々な形状で有り得る。例えば、組成物の形状は、粉状、液状、ペレット状等であり得る。また、予め種子又は植物をコーティングした形状であってもよい。コーティング方法としては従来公知のフィルムコート種子を製造する方法、鉄コーティング種子を製造する方法を適宜利用できる。また、日本国特許庁発行の標準技術集（農薬製剤技術）の「固体担体」に記載の方法を利用して組成物を調製してもよい。
<Shape of composition>
The shape of the composition for promoting plant growth according to an embodiment of the present invention is not particularly limited, and may be various shapes. For example, the composition may be in the form of powder, liquid, pellet, or the like. Moreover, the shape which coated the seed or the plant previously may be sufficient. As a coating method, a conventionally known method for producing film-coated seeds and a method for producing iron-coated seeds can be appropriately used. In addition, the composition may be prepared by using the method described in “Solid Carrier” in the standard technology collection (agrochemical formulation technology) published by the Japan Patent Office.
＜植物の成長促進用組成物の製造方法＞
本発明に係る植物の成長促進用組成物の製造方法は、Geminibasidium（ジェミニバシディウム）属の耐熱性カビを製剤化する製剤化工程を含む。
<Method for producing composition for promoting plant growth>
The method for producing a composition for promoting plant growth according to the present invention includes a formulation step of formulating a heat-resistant mold belonging to the genus Geminibasidium.
本発明の一実施形態に係る製剤化工程は特に限定されるものではなく、公知の方法により製剤化することができる。 The formulation process according to an embodiment of the present invention is not particularly limited, and can be formulated by a known method.
本発明の一実施形態に係る製剤化工程は、好ましくは、前記製剤化工程は、５０℃以上、７５℃以下の熱処理工程を含む。前記熱処理工程により、耐熱性カビを生かしつつ、他のカビ、細菌を死滅させることができる。 The formulation step according to an embodiment of the present invention preferably includes a heat treatment step of 50 ° C. or higher and 75 ° C. or lower. By the heat treatment step, other molds and bacteria can be killed while making use of heat-resistant molds.
＜植物の成長促進方法＞
本発明に係る植物の成長促進方法は、植物を生育させるための培地に、前述した植物の成長促進用組成物を供給する供給工程を含む。これにより、植物の成長を促進させることができる。
<Plant growth promotion method>
The plant growth promotion method according to the present invention includes a supplying step of supplying the above-described plant growth promotion composition to a medium for growing a plant. Thereby, the growth of a plant can be promoted.
＜培地＞
本発明に係る植物の成長促進方法で用いる培地の実施形態は、植物を生育可能なものであればよい。例えば、培地の一実施形態は、土壌、土壌改良剤およびゲル等の固形培地、水耕栽培用の液体等の液体培地で有り得る。当業者は、適宜、植物に応じた培地を選択できる。
<Medium>
The embodiment of the medium used in the plant growth promotion method according to the present invention only needs to be capable of growing plants. For example, one embodiment of the medium may be a solid medium such as soil, soil conditioner and gel, or a liquid medium such as a liquid for hydroponics. A person skilled in the art can appropriately select a medium according to the plant.
＜土壌＞
培地について土壌を用いる場合、土壌のｐＨは３以上、８以下であることが好ましい。
<Soil>
When soil is used for the medium, the pH of the soil is preferably 3 or more and 8 or less.
＜供給工程＞
供給工程は、培地に、本発明に係る組成物を供給する工程であればよい。供給方法は、例えば、培地に組成物を振りかける方法でもよく、混合する方法でもよい。例えば、土壌に培地を漉き込む方法でもよい。また、例えば、土壌の上から振り掛ける等の方法でもよい場合もある。本発明に係る組成物を培地に供給するとき、適宜他の物と混合してもよい。例えば、本発明に係る組成物を、従来公知の植物を生育するために用いる、植物ホルモン等の薬剤および／または肥料等と混合して、培地に供給してもよい場合もある。また、本発明に係る組成物に含まれる耐熱性カビが既に存在する培地に、本発明に係る組成物をさらに供給してもよい。このような供給の態様でも、植物の成長をさらに促進させることができる。また、供給工程においては、本発明に係る組成物を、当該組成物中の耐熱性カビが増殖可能なように供給すればよく、その供給量は対象の植物を生育させる環境等から当業者であれば適宜設定することができる。
<Supply process>
The supply process should just be a process of supplying the composition which concerns on this invention to a culture medium. The supply method may be, for example, a method of sprinkling the composition on the medium or a method of mixing. For example, a method of pouring a culture medium into soil may be used. Moreover, for example, a method of sprinkling from above the soil may be used. When the composition according to the present invention is supplied to the culture medium, it may be appropriately mixed with other products. For example, the composition according to the present invention may be mixed with drugs such as plant hormones and / or fertilizers used for growing conventionally known plants and supplied to the medium. Moreover, you may further supply the composition which concerns on this invention to the culture medium in which the heat-resistant mold | fungi contained in the composition which concerns on this invention already exist. Even in such a supply mode, plant growth can be further promoted. Further, in the supplying step, the composition according to the present invention may be supplied so that the heat-resistant mold in the composition can grow, and the supply amount is determined by those skilled in the art from the environment in which the target plant is grown. It can be set as appropriate.
＜供給時期＞
組成物を供給する時期は、植物の種を播く前が好ましく、また、苗木および樹木を植樹する前が好ましい。例えば、本発明に係る組成物を予め混合した土壌に、播種すること、又は苗木および植木を植樹したりすることによって、供給を行ってもよい。
<Supply time>
The timing for supplying the composition is preferably before sowing the seeds of the plant, and before planting the seedlings and trees. For example, you may supply by seed | inoculating to the soil which mixed the composition concerning this invention previously, or planting a seedling and a plant.
＜耐熱性カビが定着している植物＞
Geminibasidium（ジェミニバシディウム）属の耐熱性カビが定着した植物も、本発明の範疇である。なお、本明細書において、「定着している」とは、カビが植物組織内に菌糸が存在する状態を指す。植物は、特に限定されないが、ハクサイ、トマト、キュウリ、エンドウ、及びイチゴからなる群から選ばれる少なくとも一つであり得る。
<Plants with heat-resistant mold>
Plants to which heat-resistant molds of the genus Geminibasidium are established are also within the scope of the present invention. In the present specification, “fixed” refers to a state in which fungi are present in the plant tissue. The plant is not particularly limited, and may be at least one selected from the group consisting of Chinese cabbage, tomato, cucumber, pea, and strawberry.
本発明は上述した各実施形態に限定されるものではなく、請求項に示した範囲で種々の変更が可能であり、異なる実施形態にそれぞれ開示された技術的手段を適宜組み合わせて得られる実施形態についても本発明の技術的範囲に含まれる。 The present invention is not limited to the above-described embodiments, and various modifications are possible within the scope shown in the claims, and embodiments obtained by appropriately combining technical means disclosed in different embodiments. Is also included in the technical scope of the present invention.
以下に実施例を示し、本発明の実施の形態についてさらに詳しく説明する。もちろん、本発明は以下の実施例に限定されるものではなく、細部については様々な態様が可能であることは言うまでもない。 Examples will be shown below, and the embodiments of the present invention will be described in more detail. Of course, the present invention is not limited to the following examples, and it goes without saying that various aspects are possible in detail.
耐熱性カビとしては、Devriesia shelburniensis（デブリエシア シェルバーニエンシス）ＢＦＦ３１３（受託番号：ＮＩＴＥ ＢＰ−０２３９４）、Leohumicola verrucosa（レオフミコラ ヴェルコサ）ＢＦＦ３５２（受託番号：ＮＩＴＥ ＢＰ−０２４６７）、又はGeminibasidium donsium（ジェミニバシディウム ドンシウム）ＢＦＦ４１０（受託番号：ＮＩＴＥ ＢＰ−０２３９５）を用いた。以下、Devriesia shelburniensis（デブリエシア シェルバーニエンシス）ＢＦＦ３１３をＢＦＦ３１３、Leohumicola verrucosa（レオフミコラ ヴェルコサ）ＢＦＦ３５２をＢＦＦ３５２、Geminibasidium donsium（ジェミニバシディウム ドンシウム）ＢＦＦ４１０をＢＦＦ４１０とも称する。 As heat-resistant mold, Devriesia shelburniensis BFF313 (Accession number: NITE BP-02394), Leohumicola verrucosa BFF352 (Accession number: NITE BP-02467), or Geminibasidium donium Donsium) BFF410 (Accession Number: NITE BP-02395) was used. Hereinafter, Devriesia shelburniensis BFF313 is also referred to as BFF313, Leohumicola verrucosa BFF352 BFF352, and Geminibasidium donsium BFF410 as BFF410.
植物としては、ハクサイ、トマト、キュウリ、エンドウ、及びイチゴの種を用いた。 As the plant, Chinese cabbage, tomato, cucumber, pea, and strawberry seeds were used.
シャーレに耐熱性カビを供給し、耐熱性カビをシャーレ全体に繁殖させた。対照としては、耐熱性カビを供給しなかったシャーレを用いた。それぞれのシャーレ上に、上述した植物の発根した種子を移植し、生育させ、植物の成長促進用組成物による植物の成長促進効果を調べた。実験の詳細は、文献「エンドファイトの働きと使い方」（社団法人 農山漁村文化協会）の１０２頁から１０３頁に記載の通りである。ハクサイ、トマト、キュウリ、エンドウ、及びイチゴは、それぞれ移植から２週間生育させて、以下の試験に供した。 Heat-resistant mold was supplied to the petri dish, and the heat-resistant mold was propagated throughout the petri dish. As a control, a petri dish that did not supply heat-resistant mold was used. The seeds rooted with the plants described above were transplanted and grown on each petri dish, and the plant growth promoting effect of the plant growth promoting composition was examined. Details of the experiment are as described on pages 102 to 103 of the document “Work and Usage of Endfight” (Agricultural and Mountain Fishing Village Cultural Association). Chinese cabbage, tomatoes, cucumbers, peas, and strawberries were each grown for 2 weeks after transplantation and subjected to the following tests.
生育させた植物体の乾燥重量、外観、高さ、葉数、葉長、及び葉幅を調べた。植物体の乾燥重量は、後述した通りに行った。結果を図１〜１０、及び表１に示した。図１、図２はハクサイの結果を示し、図３、図４はトマトの結果を示し、図５、図６はキュウリの結果を示し、図７、図８はエンドウの結果を示し、図９、図１０はイチゴの結果を示す。 The dry weight, appearance, height, number of leaves, leaf length, and leaf width of the grown plants were examined. The dry weight of the plant body was performed as described later. The results are shown in FIGS. 1 and 2 show the results of Chinese cabbage, FIGS. 3 and 4 show the results of tomato, FIGS. 5 and 6 show the results of cucumber, FIGS. 7 and 8 show the results of pea, and FIG. FIG. 10 shows the results for strawberries.
〔植物体の乾燥重量測定〕
生育させた植物体の地上部を採取し、それぞれ６０℃にて３〜４日間乾燥させ、その重量を測定した。
The above-ground parts of the grown plants were collected, dried at 60 ° C. for 3 to 4 days, and their weights were measured.
表１において、ＢＦＦ３５２、ＢＦＦ３１３、ＢＦＦ４１０は、それぞれの耐熱性カビを含む植物の成長促進用組成物を用いた結果を示す。表１において、それぞれの耐熱性カビを含む植物の成長促進用組成物を供給した実施例の成長促進効果が、対照に比べて「−」は１．０倍以下、「●」は１．０倍より高く、１．５倍以下、「○」は１．５倍より高く、２．５倍以下、「◎」は２．５倍より高かったことを示す。 In Table 1, BFF352, BFF313, and BFF410 show the results of using the plant growth promoting composition containing each heat-resistant mold. In Table 1, the growth promotion effect of the Example which supplied the growth promotion composition of the plant containing each heat-resistant mold | fungi is 1.0 times or less compared with a control | contrast, and "●" is 1.0 times. It is higher than fold, 1.5 times or less, “◯” is higher than 1.5 times, 2.5 times or less, and “◎” indicates higher than 2.5 times.
図１〜１０及び表１に示すように、ＢＦＦ３５２、又はＢＦＦ３１３を含む植物の成長促進用組成物を供給することで、試験に供した植物すべての乾燥重量、及びイチゴの高さ、葉数、葉長、及び葉幅が対照に比べて増加した。 As shown in FIGS. 1 to 10 and Table 1, by supplying a composition for promoting growth of a plant containing BFF352 or BFF313, the dry weight of all the plants subjected to the test, and the height of strawberry, the number of leaves, Leaf length and leaf width increased compared to controls.
図１〜１０及び表１に示すように、ＢＦＦ４１０を含む植物の成長促進用組成物を供給することで、トマト以外の植物の乾燥重量、及びイチゴの高さ、葉数、葉長、及び葉幅が対照に比べて増加した。 As shown in FIGS. 1 to 10 and Table 1, by supplying a plant growth promoting composition containing BFF410, the dry weight of plants other than tomatoes, and the height of strawberry, the number of leaves, the leaf length, and the leaves The width increased compared to the control.
〔カビ毒４種類の有無の判定方法〕
生育させた植物について、アフラトキシン、パツリン、デオキシニバレノール及びオクラトキシンＡのカビ毒４種類の有無を調べた。以下の通りに測定を行った。
[Method for determining the presence or absence of four types of mold poisons]
The grown plants were examined for the presence or absence of four types of mold poisons such as aflatoxin, patulin, deoxynivalenol, and ochratoxin A. Measurements were made as follows.
アフラトキシン、デオキシニバレノール及びオクラトキシンＡについては、ＲＩＤＡＳＣＲＥＥＮ（登録商標）ＦＡＳＴキット（それぞれ、ＦＡＳＴ アフラトキシン （４１６１ＰＦ）、ＦＡＳＴ ＤＯＮ （４３３１ＰＦ）、ＦＡＳＴ オクラトキシンＡ（４１２２ＰＦ）、ｒ−Ｂｉｏｐｈａｒｍ ＡＧ社製、ダルムシュタット、ドイツ）を用いて、製造者の指示する手順に従って、行なった。 For aflatoxin, deoxynivalenol and ochratoxin A, RIDSCREEN (registered trademark) FAST kit (FAST aflatoxin (4161PF), FAST DON (4331PF), FAST ochratoxin A (4122PF), manufactured by r-Biopharm AG, Darmstadt, Germany) according to the manufacturer's instructions.
パツリンについては次の手順で行なった。まず、パツリンの抽出を、ＡＯＡＣ２０００に従って行なった。サンプルのスラリー５ｍｌに、エチルアセテート１０ｍｌを加えた。次に、ボルテックスミキサーを使用して、１分間、激しく撹拌した。次に、上澄みを培養試験管に移した。この工程を３回繰り返した。そして、エチルアセテート相に２ｍｌの１．５％のＮａ２ＣＯ３を加えた。そして、３０秒間激しく撹拌した。上側のエチルアセテート相を、１５ｇのＮａ２ＳＯ４中に通過させた。そして、上側のエチルアセテート相を、４５℃で、窒素蒸気下で、蒸発させた。乾燥した残渣をＨＰＬＣ分析用の酢酸２ｍｌ中に溶解させた。パツリンのＨＰＬＣでの分析条件は以下の通りとした。
・パツリン標準溶液（１００μｇ／ｍｌアセトニトリル溶液）はＷａｋｏ Ｐｕｒｅ Ｃｈｅｍｉｃａｌ Ｉｎｄｕｓｔｒｉｅｓ, Ｌｔｄから購入したものを用いた。
・パツリンを、２７６ｎｍで、ＵＶ検出器セットを備えるＷａｔｅｒｓ Ａｌｌｉａｎｃｅ（登録商標）ＨＰＬＣ Ｓｙｓｔｅｍを使用して測定した。
・３５℃で、Ｐｕｒｅｓｉｌ Ｃ１８ カラム（２５０×４．６ｍｍ、５μｍ;水）を、パツリンを分離するために使用した。
・アイソクラティック移動相は、流速１ｍｌ／分で、９７：３の割合の、０．０９５％の過塩素酸溶液−アセトニトリルであった。
For patulin, the following procedure was used. First, patulin was extracted according to AOAC2000. To 5 ml of the sample slurry, 10 ml of ethyl acetate was added. Next, it was vigorously stirred for 1 minute using a vortex mixer. The supernatant was then transferred to a culture tube. This process was repeated three times. Then 2 ml of 1.5% Na 2 CO 3 was added to the ethyl acetate phase. And it stirred vigorously for 30 seconds. The upper ethyl acetate phase was passed through 15 g Na 2 SO 4 . The upper ethyl acetate phase was then evaporated at 45 ° C. under nitrogen vapor. The dried residue was dissolved in 2 ml of acetic acid for HPLC analysis. The analysis conditions of Patulin in HPLC were as follows.
-The patulin standard solution (100 microgram / ml acetonitrile solution) used what was purchased from Wako Pure Chemical Industries, Ltd.
Patulin was measured at 276 nm using a Waters Alliance® HPLC System equipped with a UV detector set.
• At 35 ° C., a Puresil C18 column (250 × 4.6 mm, 5 μm; water) was used to separate patulin.
The isocratic mobile phase was 0.095% perchloric acid solution-acetonitrile in a 97: 3 ratio at a flow rate of 1 ml / min.
以上の結果、ＢＦＦ３５２、ＢＦＦ３１３、ＢＦＦ４１０のいずれも毒性は陰性であった。 As a result, all of BFF352, BFF313, and BFF410 were negative in toxicity.
ＮＩＴＥ ＢＰ−０２３９４
ＮＩＴＥ ＢＰ−０２３９５
ＮＩＴＥ ＢＰ−０２４６７
NITE BP-02394
NITE BP-02395
NITE BP-02467
Claims (7)
前記Geminibasidium（ジェミニバシディウム）属の耐熱性カビは、Geminibasidium donsium（ジェミニバシディウム ドンシウム）である、植物の成長促進用組成物。 The composition for promoting plant growth according to claim 1,
The composition for promoting growth of a plant, wherein the heat-resistant mold belonging to the genus Geminibasidium is Geminibasidium donsium (Geminibasidium donsium).
賦形剤をさらに含む、植物の成長促進用組成物。 The plant growth promoting composition according to claim 1 or 2,
A composition for promoting plant growth, further comprising an excipient.
前記製剤化工程は、５０℃以上、７５℃以下の熱処理工程を含む、植物の成長促進用組成物の製造方法。 In the manufacturing method of the composition for plant growth promotion of Claim 5,
The said formulation process is a manufacturing method of the composition for plant growth promotion including the heat processing process of 50 to 75 degreeC.
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