JP2019064961A - Repellent for anthocoridae - Google Patents

Abstract

To provide a repellent effective for migrating genus Orius Anthocoridae, which is a predator of fine pest insect such as aphid, thrips, or spider mite, and promising natural enemy insect from natural enemy preservation plant to crop.SOLUTION: There is provided a repellent for genus Orius Anthocoridae, containing (E)-2-octenal as an active component, and a method for promoting genus Orius Anthocoridae discreteness behavior by scattering or installing the repellent.SELECTED DRAWING: None

Description

  The present invention relates to a repellent against a stink bug that belongs to the genus Orius. The present invention also relates to a method for promoting the discrete behavior of a coatworm belonging to the genus Orius, comprising spraying or setting up the repellent agent.

  Stink bug (Orius genus) is a predator of small pests such as aphids, thrips and red mites, and is expected globally as a promising natural enemy insect. Orius sauteri is a bryophyte commonly found all over Japan. As part of natural enemy utilization and protection, mixed planting of selective drugs in the field and plants called natural enemy-sparing plants has been carried out (Non-Patent Documents 1 to 3). Recently, it has been proposed that a combination of a light source having a specific wavelength and a natural enemy-sparing plant be used to efficiently introduce the beetle to agroecosystem (Non-Patent Document 4). As a result, the stink bug can be effectively retained and maintained on natural enemy-sparing plants. However, it has been difficult to transfer the stink bug from a natural enemy saving plant to a crop. Keeping the stink bug on natural enemy sparing plants for a long time can significantly reduce the predatory efficiency of pests on the crop.

  It is known that B. tabaci uses the semiochemicals emitted by other species or plants for searching for food and mating behavior. For example, B. tabaci is considered to prefer the smell of pollen of crops and natural enemy-sparing plants and the odor released from crops that are poisoned by this thrips (Non-patent Documents 5 to 7). The males of O. insidossus that live in North America are attracted to a mixture of (E) -2,7-octadienal and (E) -2-octenal, which are female sex pheromone components. A long-chain unsaturated hydrocarbon is identified as a footprint sex pheromone in O. minutus, and it is known that males stay for a long time in the place where female footprints remain (Non-patent Document 8). Although O. insidiosus and O. sauteri also suggest the presence of footprint sex pheromone, no substance has been clarified (Non-patent Documents 9 and 10). As mentioned above, although the relation between various behaviors of B. tabaci and semiochemicals is suggested, few have known the substance.

  Push-pull strategy (Push-pull strategy) was proposed by Cook et al. As a pest management method achieved by combining (pulling) and pushing (pushing) pests and their natural enemy insects into the field Patent Document 11). Push-pull strategies generally consist of behavioral control techniques through sensory stimuli such as vision and chemoreception. In pests, both techniques of attraction and repellent behavior are well studied. However, in natural enemy insects, research is limited to techniques related to attractive behavior, and research related to repellent behavior has hardly been studied.

  Patent Document 1 describes a repellent against spider beetles containing (E) -2-octenal. However, the spider bug (Leptocorisa chinensis) is an insect belonging to the family Hemiptera Hosohelidae, and a family of the family Bacteriidae to which the flower bug belongs is a different type of insect at the "family" level.

JP 2005-68021 A

Nagai, K., Jpn. J. Appl. Ent. Zool., 34: 109-114 (1990) Imura, T. and Kamikawa, S., Ann. Rept. Kansai. Pl. Prot., 2012, 54: 163-165 (2012) Ohta, I., and Takeda, M., Ann. Rept. Kansai Pl. Prot., 56: 1-5 (2014) Ogino, T., et al., Sci. Rep., Vol. 6, Article number 32302 (2016), doi: 10.1038 / srep 32302 Carvalho, L. M., et al., J. Appl. Entomol., 135: 177-183 (2011) Mochizuki, M. and Yano, E., Entomol. Exp. Appl., 123: 57-62 (2007) Xu, X. and Enkegaard, A., Entomol. Exp. Appl., 132: 93-98 (2009) Maeda, T., et al., J. Chem. Ecol., 42: 433-443 (2016) Aldrich, JR, et al., J. Chem. Ecol., 33: 1477-1493 (2007) Nakashima, Y. and Hirose, Y., Ecol. Entomol., 24: 115-117 (1999) Cook, S. M., et al., Ann. Rec. Entomol., 52: 375-400 (2007)

  As mentioned above, keeping the bug on a natural enemy-sparing plant for a long time may significantly reduce the predatory efficiency of pests on the crop, while transferring the flowering bug on a natural enemy-sparing plant to a crop It was difficult to As a solution to these problems, a behavioral control method of the stink bug is required.

  The present invention provides a repellent against B. tabarius belonging to the genus Orius, and a method for promoting discrete behavior of B. tabarius belonging to the genus Orius, which comprises spraying or setting the repellent.

  In view of the above, the inventor of the present case focused on the extract of the bug of the stink bug and started research. As a result of intensive investigations, it was found that (E) -2-octenal promotes the discrete behavior of the stink bug. The present invention has been completed based on the findings.

That is, in one aspect, the present invention may be as follows.
[1] A repellant against the leaf beetle that belongs to the genus Orius (Orius), which comprises (E) -2-octenal as an active ingredient.

  [2] The repellent agent according to the above-mentioned [1], wherein the flowering beech beetle belonging to the genus Orius is a flowering beetle, a flowering beetle, a flowering beetle, a flowering beetle, a flowering beetle, or a flowering beetle.

  [3] A method for promoting the discrete behavior of the flower beetle belonging to the genus Orius, which comprises treating with the repellent described in the above-mentioned [1] or placing the repellent.

  The repellent agent of the present invention can promote the discrete behavior of the flower beetle belonging to the genus Orius.

FIG. 1 is a graph showing the results of evaluating the concentration dependency of the discrete effect of (E) -2-octenal.

  The present invention will be specifically described below, but the present invention is not limited thereto. Unless otherwise defined herein, scientific and technical terms used in connection with the present invention shall have the meanings that are commonly understood by those of ordinary skill in the art.

The present invention provides a repellent agent against the flower beetle which belongs to the genus Orius, which comprises (E) -2-octenal as an active ingredient.
There are no particular limitations on the bug which belongs to the genus Orius, as long as it is an insect which belongs to the genus Orius. In a preferred embodiment, the stink bug belonging to the genus Orius is Orius sauteri, Orius strigicollis, Orius minutus, Origami moth (Oriusu nagaii), and / or It may be a southern leaf beetle (Orius tartillus). In a particularly preferred embodiment, the coatworm belonging to the genus Orius may be Orius sauteri.

  The repellent of the present invention may contain only (E) -2-octenal, or may be a solid carrier, a liquid carrier, a carrier such as a gaseous carrier, a surfactant, and other adjuvants for formulation. Furthermore, you may contain. The amount of (E) -2-octenal contained in the repellent agent of the present invention is not particularly limited, and may be 0.01 to 100% by weight of (E) -2-octenal.

  The form of the repellent agent of the present invention is not particularly limited as long as it is a form capable of scattering or releasing (E) -2-octenal, but it is a solid preparation, solution, microemulsion, oil, emulsion, wettable powder, wettable granules Agents, suspension in water, flowable agents such as emulsions in water, powders, granules, tablets, microcapsules, aerosols, heated fumigants, heated transpiration agents and the like.

  The solid carrier is not particularly limited as long as it is an acceptable carrier in the formulation of agricultural chemicals, and examples thereof include filter paper, cotton, clays (kaolin clay, diatomaceous earth, bentonite, humus clay, acid clay etc), synthetic hydrous silicon oxide, talc Powders and particles of metals, ceramics, other inorganic minerals (sericite, quartz, sulfur, activated carbon, calcium carbonate, hydrated silica etc.), chemical fertilizers (ammonium sulfate, ammonium phosphate, ammonium nitrate, urea nitrate, ammonium chloride etc.) , High molecular weight materials (polyethylene, polypropylene, polyester, nylon, polyvinyl alcohol, polyvinyl acetate saponified, polyvinylidene chloride (PVDC), ethylene / vinyl alcohol copolymer, ethylene / vinyl acetate copolymer saponified, PVDC coated Film or sheet, aluminum evaporation foil Beam or sheet), and the like.

  The liquid carrier is not particularly limited as long as it is an acceptable carrier for formulation of agricultural chemicals, but, for example, water, alcohols (methanol, ethanol etc.), ketones (acetone, methyl ethyl ketone etc.), aromatic hydrocarbons (toluene, Xylene, ethylbenzene, methylnaphthalene etc.), non-aromatic hydrocarbons (hexane, cyclohexane, kerosene, light oil etc.), esters (ethyl acetate, butyl acetate etc.), nitriles (acetonitrile, isobutyronitrile etc.) ethers Acidic amides (N, N-dimethylformamide, N, N-dimethylacetamide etc.), halogenated hydrocarbons (dichloromethane, trichloroethane, carbon tetrachloride etc.), dimethyl sulfoxide, vegetable oil (soybean oil , Cottonseed oil etc.).

  The gaseous carrier is not particularly limited as long as it is an acceptable carrier for formulation of agricultural chemicals, and examples thereof include fluorocarbon gas, butane gas, liquefied petroleum gas, dimethyl ether, carbon dioxide gas and the like. Gaseous carriers may be utilized as a propellant.

  The surfactant is not particularly limited as long as it is acceptable in formulation of agrochemicals, and examples thereof include alkyl sulfates, alkyl sulfonates, alkyl aryl sulfonates, alkyl aryl ethers, and poly alkyl aryl ethers. Oxyethylene compounds, polyethylene glycol ethers, polyhydric alcohol esters, sugar alcohol derivatives and the like can be mentioned.

  Other pharmaceutical adjuvants include, for example, casein, gelatin, saccharides (starch, gum arabic, cellulose derivatives, alginic acid etc.), lignin derivatives, bentonite, synthetic water-soluble polymers (polyvinyl alcohol, polyvinyl pyrrolidone, polyacrylic acids etc.) ), PAP (isopropyl acid phosphate), BHT (2,6-di-tert-butyl-4-methylphenol), BHA (2-tert-butyl-4-methoxyphenol and 3-tert-butyl-4-methoxy) Phenols, cyclodextrins, vegetable oils, mineral oils, fatty acids, fatty acid esters.

  The solid preparation is obtained by impregnating the active ingredient (E) -2-octenal with a solid carrier such as filter paper, cotton and a polymer carrier, and the active ingredient can be continuously diffused in the air. It is a formulation in the form. The form of the solid preparation is not particularly limited. The solid preparation can be used by setting as it is.

  Solutions, microemulsions, oils and emulsions can be used neat or diluted. These formulations or their dilutions can be used by spraying or spraying. As means for dispersing or spraying these preparations or their diluted bases, those known to those skilled in the art can be used, and for example, they may be used by filling in a spray container or a sprayer.

  Wettable powders, wettable granules, and flowables are usually used after dilution with water or the like. Dilutions of these formulations can be used by spraying or spraying. The means for spraying or spraying is as described above.

Powders, granules, tablets and microcapsules can be used as they are by spraying or setting.
Aerosols are preparations in the form in which the active ingredient is packed in a suitable sprayer together with a gaseous carrier, and can be used by spraying the active ingredient.

A heating fumigant and a heating evaporation agent are preparations which gasify or atomize and disperse an active ingredient by heating.
The repellent agent of the present invention is applied (treatment such as spraying, spraying or dispersing, or placed) in an area where the discrete behavior of B. tabaci is to be promoted, such as a natural enemy-sparing plant and its surroundings. Thus, the present invention provides a method for promoting the discrete behavior of B. tabarius belonging to the genus Orius, which comprises treating with the repellent agent of the present invention or placing the repellent agent.

The application amount of the repellent agent of the present invention is not particularly limited as long as it promotes the discrete behavior of the stink bug. For example, you may determine suitably, observing the discrete behavioral state of a stink bug. Usually, per 1m ² (E) -2- octenal is applied such that 0.1 mg to 100 mg. Or you may apply so that (E) -2- octenal may be 100 micrograms or more per one point to apply.

Hereinafter, the present invention will be described in more detail by way of examples, but these are for the purpose of illustration and do not limit the present invention.
Example 1: Identification and evaluation of substances promoting discrete behavior of the stink bug
1. Materials and methods
Test insect
In 2011, it was captured in Tsukuba City, Ibaraki Prefecture, and thereafter, was maintained in the laboratory and used for experiments. Rearing was carried out in a plastic container, and eggs of Scutellaria barbata D. Don were used as bait and leaves of P. mexicanum were used for egg laying substrate and humidity control. The breeding conditions were room temperature 25 ± 1 ° C., humidity 60-70%, light condition 16 hours light period 8 hours dark period. A plastic tube filled with water was placed in a rearing container to maintain humidity.

Extraction conditions Insects were transferred into a freezer and anesthetized, then 30 individuals were transferred to glass vials and immersed in 100 μl of hexane. The glass vial was immersed in an ultrasonic cleaner and worm extraction was performed for 5 minutes at room temperature. The extract was stored in a -20.degree. C. freezer until used for experiments.

Test reagents (E) -2-octenal, (E) -2-decenal and standard hydrocarbons were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).

The structure of the components in the chemical analysis extract was determined by gas chromatography-mass spectrometry (GC-MS). GC-MS analysis was performed using a gas chromatograph Agilent 6890N (Agilent Technology) and 5973 equipped with a split / splitless injector and a DB-5 MS capillary column (30 m × 0.25 mm diam. × 0.25 μm film thickness; Agilent Technology, CA, USA) A mass detector (Agilent Technology) was performed. Helium gas was used as a carrier gas, and the flow rate was 1.1 ml / min. Temperature conditions maintained the injection port at 250 ° C. The oven was maintained at 50 ° C. for 2 minutes, then heated up to 250 ° C. at 10 ° C./minute and held at 250 ° C. for 10 minutes. GC analysis used DB-5MS and DB-23 (30 m × 0.25 mm diam. × 0.25 μm film thickness; Agilent Technologies) as columns of different polarity. A hydrogen flame detector (FID) was used as a detector. The temperature conditions were according to GC-MS analysis.

Discrete behavior evaluation (E) -2-octenal bioassay was performed in an environment of 25 ± 1 ° C. in a plastic cage (24.2 × 14.7 × 20.8 cm). The floor of the cage was covered with filter paper. For moisture control, stems and leaves of C. mexicanum were placed at the center of the floor. Insects were collected from breeding containers using an aspirator and transferred to behavioral observation cages. All experiments were performed from 8 am to 10 am when insect activity was reduced. In order to minimize the influence on the experiment by treating the insects, 10 individuals were transferred to the behavior monitoring cage Mexican manengusa on the evening of the previous day, and the plants were kept in glass petri dishes until the start of the experiment so as not to escape. I covered it. This operation made it possible to use a more settled insect for the experiment and to observe the insect's spontaneous behavior. Immediately after removing the petri dish, (E) -2-octenal (0.01 to 1000 μg) dissolved in 1 μl of hexane was dropped onto the center of the filter paper. The discrete effects on the components were evaluated based on the plant-insect distance 10 minutes after dropping the components. Individuals who left the plant before the experiment and walked the petri dishes were removed before the experiment and removed from the data.

Statistical analysis Discrete effects of (E) -2-octenal on worms were evaluated using half effective concentrations (EC ₅₀ ). (E) After treatment with -2-octenal, treated as an individual who responded to an insect at least 5 cm away from the plant, as an individual who responded to an insect remaining less than 5 cm from the plant as a response-non-response binary value Data were subjected to logistic regression analysis.

2. result
Identification larvae and male worms extracts derived material, from a comparison of the worms extract female was detected as a common component (E)-2-octenal. From the GC-MS analysis, fragment ions of the common component are m / z 125 (M ⁺ -1, 1%), 108 (4%), 97 (19%), 83 (73%), 70 (base peak) 69 (49%) and 55 (88%). Molecular ion -1 and dehydrated ion m / z 108 ([M ⁺ -18] ⁺ ) were observed. In addition, fragment ions (m / z 97, 83, 69 and 55) separated by 14 showed unsaturated linear aliphatic compounds. These results suggested the molecular formula C ₈ H ₁₄ O, consistent with the result that (E) -2-octenal is a common component. When the retention indexes in two different columns were compared, the common components were 1062 and 1492 and were in agreement with the retention indexes 1062 and 1492 of (E) -2-octenal. From these results, (E) -2-octenal was determined as a common component of larvae, males and females in the stink bug.

Discrete behavior evaluation (E) We investigated about the discrete effect of octenal. The results are shown in FIG. Before the start of the experiment, most of the insects released the day before gathered on the plant and remained. The worm did not respond well to the concentration of 0.1 μg, even when given (E) -2-octenal. However, when higher concentrations of (E) -2-octenal were given, discrete behavior from plants was observed immediately. The discrete behavior emerged from 1 μg, which corresponds to 1.6 to 6 times of (E) -2-octenal possessed by one animal. The half effective concentrations (EC ₅₀ ) of larvae, males and females are 93.78 μg (67-120.55 (95% confidence interval)), 31.34 μg (12.82-49.85), 140.14 μg, respectively. (101.16 to 179.13), males most sensitively responded to (E) -2-octenal.

  From the results of chemical analysis and behavioral evaluation, it was revealed that (E) -2-octenal is the main component of the extract of the insect body of the black flea beetle, and that it causes the discrete behavior of this species to be dependent on concentration .

The repellent agent of the present invention is to promote the discrete behavior of the flower beetle belonging to the genus Orius. So far, it has been difficult to transfer the stink bug from a natural enemy-sparing plant to a crop, but by treating the natural enemy-sparing plant with the repellent agent of the present invention, it promotes the transfer of the stink bug to a crop on the natural enemy-sparing plant It becomes possible.

Claims (3)

  (E) A repellant against the flower beetle which belongs to the genus Orius (Orius), which contains -2-octenal as an active ingredient.   The repellent agent according to claim 1, wherein the stink bug belonging to the genus Orius is a stink bug, a stink bug, a stink bug, a stink bug, a sting bug, or a stink bug. A method for promoting the discrete behavior of a flowering beetle belonging to the genus Orius, which comprises treating with the repellent agent according to claim 1 or placing the repellent agent.

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