JP2019050777A5 - Composite and its manufacturing method, laminate, cell adhesion substrate and its manufacturing method - Google Patents

Composite and its manufacturing method, laminate, cell adhesion substrate and its manufacturing method Download PDF

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JP2019050777A5
JP2019050777A5 JP2017178043A JP2017178043A JP2019050777A5 JP 2019050777 A5 JP2019050777 A5 JP 2019050777A5 JP 2017178043 A JP2017178043 A JP 2017178043A JP 2017178043 A JP2017178043 A JP 2017178043A JP 2019050777 A5 JP2019050777 A5 JP 2019050777A5
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particles
cell adhesion
composite
hydrogel containing
manufacturing
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JP2017178043A
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JP2019050777A (en
JP7102694B2 (en
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Claims (11)

細胞接着性を有する粒子を含有するハイドロゲルと、
前記粒子を含有しないハイドロゲルと、
前記粒子に接着している細胞と、を含み、
平面視した全体表面に対する、前記粒子の露出表面積の占有割合が２０％以上である、ことを特徴とする複合体。 Hydrogel containing particles with cell adhesion and
Hydrogels that do not contain the particles
Look including the, and the cells adhering to the particles,
A composite characterized in that the ratio of the exposed surface area of the particles to the entire surface in a plan view is 20% or more . 前記粒子の体積平均粒径が、前記細胞の体積平均粒径より小さい請求項１に記載の複合体。The complex according to claim 1, wherein the volume average particle size of the particles is smaller than the volume average particle size of the cells. 前記粒子の体積平均粒径が、１μｍ以下である請求項１から２のいずれかに記載の複合体。The composite according to any one of claims 1 to 2, wherein the volume average particle diameter of the particles is 1 μm or less. 前記粒子の体積平均粒径が、１００ｎｍ以上１μｍ以下である請求項３に記載の複合体。The composite according to claim 3, wherein the volume average particle diameter of the particles is 100 nm or more and 1 μm or less. 前記細胞が、前記粒子と接着点を有する請求項１から４のいずれかに記載の複合体。The complex according to any one of claims 1 to 4, wherein the cell has an adhesion point with the particle. 前記粒子が、細胞接着因子を有する請求項１から５のいずれかに記載の複合体。The complex according to any one of claims 1 to 5, wherein the particles have a cell adhesion factor. 前記粒子が、ゼラチン粒子である請求項１から６のいずれかに記載の複合体。The complex according to any one of claims 1 to 6, wherein the particles are gelatin particles. 請求項１から７のいずれかに記載の複合体を、前記複合体の厚み方向に複数有することを特徴とする積層体。A laminate characterized by having a plurality of the composite according to any one of claims 1 to 7 in the thickness direction of the composite. 細胞接着性を有する粒子を含有するハイドロゲルの領域を形成する工程と、The process of forming a region of hydrogel containing particles with cell adhesion and
前記粒子を含有しないハイドロゲルの領域を形成する工程と、The step of forming the region of the hydrogel containing no particles and
前記粒子に細胞を接着させる工程と、The step of adhering cells to the particles and
を含むことを特徴とする複合体の製造方法。A method for producing a complex, which comprises. 細胞接着性を有する粒子を含有するハイドロゲルと、Hydrogel containing particles with cell adhesion and
前記粒子を含有しないハイドロゲルと、を含み、Hydrogel containing no particles,
平面視した全体表面に対する、前記粒子の露出表面積の占有割合が２０％以上である、ことを特徴とする細胞接着用基材。A cell adhesion base material, wherein the proportion of the exposed surface area of the particles to the entire surface in a plan view is 20% or more. 細胞接着性を有する粒子を含有するハイドロゲルの領域を形成する工程と、The process of forming a region of hydrogel containing particles with cell adhesion and
前記粒子を含有しないハイドロゲルの領域を形成する工程と、The step of forming the region of the hydrogel containing no particles and
を含むことを特徴とする細胞接着用基材の製造方法。A method for producing a base material for cell adhesion, which comprises.
JP2017178043A 2017-09-15 2017-09-15 Complex and its manufacturing method, laminate, cell adhesion substrate and its manufacturing method Active JP7102694B2 (en)

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JP2019050777A JP2019050777A (en) 2019-04-04
JP2019050777A5 true JP2019050777A5 (en) 2020-09-03
JP7102694B2 JP7102694B2 (en) 2022-07-20

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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113372580A (en) * 2021-06-17 2021-09-10 西北工业大学 Preparation method of composite hydrogel and construction method of cell microenvironment bionic system

Family Cites Families (9)

* Cited by examiner, † Cited by third party
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MXPA04003162A (en) 2001-10-05 2005-01-25 Surmodics Inc Particle immobilized coatings and uses thereof.
JP5164156B2 (en) 2007-11-01 2013-03-13 独立行政法人産業技術総合研究所 Patterning cells on a substrate using inkjet printing technology
CN102458496B (en) 2009-05-15 2016-06-01 新加坡南洋理工大学 For manufacturing compositions and the production method thereof of scaffold for tissue engineering
US8715247B2 (en) 2009-07-30 2014-05-06 Carefusion 303, Inc. Collapsible valve
US9347037B2 (en) 2013-02-11 2016-05-24 Evan Masataka Masutani Methods and apparatus for building complex 3D scaffolds and biomimetic scaffolds built therefrom
EP3006559B1 (en) 2013-05-31 2019-11-06 iHeart Japan Corporation Layered cell sheet incorporating hydrogel
KR20160026899A (en) 2013-06-06 2016-03-09 에스엔에스 나노 피버 테크놀로지, 엘엘씨 Three-dimensional structures for cell or tissue culture
JP6519168B2 (en) 2014-12-17 2019-05-29 株式会社リコー Three-dimensional cell assembly, method for producing the same, and solution for forming the same
JP2017131144A (en) 2016-01-27 2017-08-03 株式会社リコー Materials for preparing three-dimensional cell aggregates, compositions for preparing three-dimensional cell aggregates, three-dimensional cell aggregate preparation sets, composition-receiving containers, and production methods of three-dimensional cell aggregates

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