JP2019047769A - Production method of culture vessels, and production method of parts for culture vessels - Google Patents

Abstract

To provide methods for easily and rapidly producing culture vessels corresponding to various incidental facilities, and to provide methods for producing parts for culture vessels.SOLUTION: A production method of a culture vessel comprises: modeling a part 24 of a culture vessel containing ABS resin as a main material using a three-dimensional molding apparatus that laminates a material melted with heat to mold a three-dimensional object; applying to the part 24 a coating material 26 that does not affect the culture of interest; curing the coating material; and attaching a part 22 to the culture vessel.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a method for producing a culture container used for culturing cells, microorganisms and the like, and a method for producing a component for a culture container.

  Technologies for using cells and microorganisms are spreading mainly in the fields of medicine and food. In this technique, a target substance is often produced while culturing cells, microorganisms and the like in a culture solution, and usually, the culture process is performed while adjusting the state of the culture solution. Therefore, in a container (culture container) used for culture, additional equipment such as sampling means for extracting the culture solution, various sensors, gas supply means for supplying various gases, and stirring for stirring the culture medium It is common for the wings to be attached.

JP, 2015-216923, A

Accessory equipment attached to the culture vessel is selected according to the target culture. Therefore, the culture vessel is preferably configured to be compatible with various incidental facilities. In general, incidental equipment is attached to a culture vessel via a connection part, and in order to cope with a wide variety of incidental equipment, it is necessary to have a wide variety of connected parts.
In addition, the culture step is often carried out while stirring the culture medium, and therefore, a stirring blade is generally attached to the culture vessel. Also for this stirring blade, since the optimal shape changes depending on the culture solution, culture object, and culture conditions, it is preferable to have many types of blades.
Here, if the connection parts and the stirring blades are made of, for example, a metal such as stainless steel, parts suitable for the purpose can be made, but it has been difficult to reduce the manufacturing cost. Moreover, if the said connection parts and the stirring blade are produced using a metal mold | die, although a manufacturing cost can be reduced, it was difficult to respond | correspond to a small amount and many kinds.
Also, in recent years, development of three-dimensional modeling apparatus has been advanced, and is being used for manufacturing various parts. Although this makes it possible to produce many kinds of parts in small quantities, the materials used for the three-dimensional modeling apparatus are limited, and the conditions necessary for culture (fastness, water resistance, sterilization resistance・ There is no material that satisfies all the effects on culture, etc.

  An object of the present invention is to provide a method of manufacturing a culture container capable of easily and quickly manufacturing containers corresponding to various incidental facilities, and a method of manufacturing parts of the culture container.

(1) The method for producing a culture vessel according to the present invention,
Forming a culture vessel part mainly composed of an ABS resin using a three-dimensional forming apparatus for forming a three-dimensional object by laminating heat-melted materials;
Applying a coating material that does not affect the target culture on the part;
Curing the coating material;
Attaching the part to a culture vessel;
including.

  According to the method of manufacturing the culture container, it is possible to easily and quickly manufacture containers corresponding to various incidental facilities.

In the method of manufacturing this culture vessel,
The step of attaching the component to the culture vessel comprises
Placing the component at the opening of the culture vessel;
Applying the coating material between the opening and the part;
A second curing step of curing the coating material;
May be included.

(3) The manufacturing method of this culture vessel component is
Forming a culture vessel part mainly composed of an ABS resin using a three-dimensional forming apparatus for forming a three-dimensional object by laminating heat-melted materials;
Applying a coating material that does not affect the target culture on the part;
Curing the coating material;
including.

  According to the manufacturing method of this culture container, it becomes possible to manufacture many kinds of parts which can be used for culture little by little.

The figure which shows an example of the culture container manufactured by the method which concerns on this Embodiment. The figure which shows the manufacturing method of the culture container which concerns on this Embodiment.

  Hereinafter, an embodiment to which the present invention is applied will be described with reference to the drawings. However, the present invention is not limited to the following embodiments. That is, all the configurations described in the following embodiments are not necessarily essential to the present invention. Furthermore, the present invention includes any combination of the following contents.

  A method of manufacturing a culture vessel according to the embodiment of the present invention will be described with reference to FIGS. 1 to 2C.

(1) Configuration of Culture Container The culture container 100 shown in FIG. 1 is an example of the culture container manufactured by the method according to the present embodiment. Hereinafter, the configuration of the culture container 100 will be described.

  The culture vessel 100 is a member on which the target culture is performed, and the volume, material, and shape of the culture container 100 are preferably optimized for the target culture. Then, various ancillary facilities are connected to the culture vessel 100 in order to maintain the internal environment (culture fluid) in a state suitable for culture. Examples of ancillary equipment include gas supply means for supplying various gases to the culture solution, various sensors for sensing the state of the culture solution, culture solution supply means for supplying the culture solution, and culture solution. The culture solution extraction means (sampling means) for extracting is mentioned. And the culture container 100 is equipped with various connection components for connecting these incidental facilities.

The culture vessel 100 has a culture tank 10. The culture tank 10 is a member that holds the culture solution inside, and the shape, capacity, and material of the culture tank 10 are appropriately selected according to the purpose of culture. In the present embodiment, the culture vessel 10 has a circular bottom surface 12 and a cylindrical side surface 14. The bottom surface 12 is configured with a plurality of openings to allow attachment of various components.
Although the constituent material of the culture tank 10 is not specifically limited, In this Embodiment, the culture tank 10 is comprised with the polycarbonate material. Polycarbonate materials are less expensive and easier to process than rigid materials such as glass and stainless steel, so it is possible to inexpensively manufacture culture vessels of a shape that matches the purpose. However, polycarbonate materials are generally unsuitable for repeated use on the premise of multiple sterilizations because they are inferior in strength. Therefore, the culture tank 10 is configured as a so-called single-use type culture vessel. However, separately from this, the culture vessel can be made of a rigid material such as glass or stainless steel, and in this case, culture can be performed multiple times by using steam sterilization or the like.
In the present embodiment, the culture tank 10 is supported by the support member 16. As a result, an appropriate space can be provided below the bottom surface 12, so that the culture vessel 100 can be easily handled even in the state where the nozzle 22 described later is attached to the bottom surface 12.

  In the present embodiment, a nozzle 22 for supplying a gas to the culture tank 10 is attached to the bottom surface 12 of the culture tank 10. The nozzle 22 is formed with a hole communicating with the inside and the outside of the culture tank 10, and the gas can be supplied to the inside of the culture tank 10 through the hole. The nozzle 22 is a member serving as a supply port for supplying, for example, oxygen, carbon dioxide, nitrogen, a mixed gas thereof, or sanitary air to the culture tank 10, and the number and shape thereof are suitable for the target culture. Is selected. Specifically, a through hole is formed in the nozzle 22, one of the through holes is opened in the culture tank 10, and the other is connected to the gas supply means. By setting the shape of the nozzle in accordance with the gas supply means, it is possible to supply gas to the culture tank 10 using various gas supply means. In the present embodiment, the porous body 23 is attached to the nozzle 22. By supplying a gas to the culture solution through the porous body 23, it is possible to cause the culture solution to release air bubbles with a small diameter. In the present embodiment, a seal member can be provided between the opening of the bottom surface 12 and the nozzle 22 (not shown). This can prevent the culture solution from leaking out from the bottom surface 12 of the culture tank 10.

The culture vessel 100 has a top 30. The top plate 30 is a member which blocks the upper opening of the culture tank 10 (side surface 14). The top plate 30 can be made of, for example, a polycarbonate material. The top plate 30 is formed with a plurality of openings to enable attachment of various components.
In the present embodiment, the culture medium supply nozzle 32 and the sensor 34 are attached to the top plate 30. The culture medium supply nozzle 32 is attached to the opening of the top plate 30 via the adapter 36, and it is possible to supply the culture medium from the outside of the culture vessel 100 via the culture medium supply nozzle 32. Moreover, the sensor 34 is a member which measures the state of a culture solution, Comprising: A temperature sensor, an oxygen sensor, pH sensor etc. are mentioned, for example. In the present embodiment, the sensor 34 is attached to the opening of the top 30 through the adapter 38. Moreover, in the present embodiment, a plurality of sensors can be attached to the top plate 30. A signal from the sensor 34 is sent to the control unit via a cable, and culture control is performed based on this.

The culture vessel 100 has a stirring blade 42. The stirring blade 42 plays a role of stirring the culture solution in the culture vessel 100. The stirring blade 42 is attached to the stirring shaft 44 and configured to be rotatable about the stirring shaft 44. The shape and the number of the stirring blades 42 are not particularly limited, but the stirring blades 42 are preferably configured to give a desired liquid flow (liquid flow suitable for the target culture) to the culture solution.
In addition, the system which rotationally drives the stirring blade 42 is not specifically limited. For example, by making the stirring blade 42 into the structure which the magnet was embedded, using the magnetic force, the stirring blade 42 can be rotated from the tank outside. Alternatively, the stirring blade can be rotated by driving the stirring shaft 44 to rotate.

(2) Method of Manufacturing Culture Container Next, a method of manufacturing a culture container according to the embodiment of the present invention will be described.
The method for producing a culture vessel includes the step of producing a part for culture vessel. Here, with reference to FIGS. 2A to 2C, a method of manufacturing the nozzle 22 will be described as an example of the culture vessel component.
In the present embodiment, the method of manufacturing the culture vessel component includes the step of modeling the component 24 shown in FIG. 2 (A) using a three-dimensional modeling apparatus. In the present embodiment, a three-dimensional modeling apparatus of a type in which heat-melted materials are stacked to form a three-dimensional object is used, and the component 24 is formed mainly of an ABS resin. Thereby, it is possible to form a component 24 with sufficient robustness to withstand culture.

  The method of manufacturing the culture vessel component includes the step of applying the coating material 26 to the component 24 as shown in FIG. 2 (B). The coating material 26 utilizes the thing which does not affect the culture made into the objective. In addition, when manufacturing components suitable for culture of animal cells, for example, “LOCTITE (registered trademark) UV-visible light curable adhesive 4304” can be used as the coating material 26.

  The method of manufacturing the culture vessel component includes the step of curing the coating material 26. Thereby, as shown in FIG. 2C, a film 28 covering the component 24 is formed. In the present embodiment, the film 28 can be formed so as to cover the whole of the component 24. However, it is also possible to form the film 28 so as to cover only the portion of the component 24 that is in contact with the culture solution. It is possible. As a result, the amount of coating material 26 used can be reduced, and the manufacturing cost of parts can be reduced. Further, in the present embodiment, the film 28 is formed on the part of the component 24 which is in contact with the culture solution. This makes it possible to prevent the culture solution from seeping into the component 24.

  The nozzle 22 (part for culture container) can be manufactured by the above process. Moreover, it is also possible to manufacture the adapter 36, the adapter 38, and the stirring blade 42 by the same process.

  In the present embodiment, as the coating material 26 (coating 28), it is preferable to use a material that can be sterilized. Since this makes it possible to sterilize the entire culture container after manufacturing the culture container, it is possible to provide a component for the culture container (culture container) with a low risk of contamination in the culture step.

The manufacturing method of the culture container which concerns on this Embodiment includes the process of attaching the said components for culture containers to a culture container. Specifically, the nozzle 22 is attached to the opening of the bottom 12, and the adapter 36 and the adapter 38 are attached to the opening of the top plate 30. Moreover, the stirring blade 42 is attached to the top plate 30 via the stirring shaft 44. In this step, a step of applying the coating material 26 between the culture vessel (the bottom surface 12 and the top plate 30) and the culture vessel component (the nozzle 22, the adapters 36, 38) and curing the coating material 26 And the step of According to this, since it is possible to seal between the culture vessel and the culture vessel component, it is possible to prevent the culture solution from leaking out.
In addition, the manufacturing method of the culture container which concerns on this Embodiment may further include the process of apply | coating the coating material 26 to the junction part of the bottom face 12 and the side 14, and the process of hardening this. Thereby, since the space between the bottom surface 12 and the side surface 14 can be sealed, leakage of the culture solution can be prevented.

Then, the culture vessel 100 can be manufactured through the steps of attaching the culture medium supply nozzle 32 to the adapter 36 and attaching the sensor 34 to the adapter 38.
In addition, the process of manufacturing the culture container 100 may further include the process of sterilizing this. Although the specific method of sterilization treatment is not particularly limited, gamma sterilization, for example, can be applied as a method of sterilizing the culture container 100.

(3) Effects Hereinafter, the functions and effects of the present embodiment will be described.
In the present embodiment, parts of the culture vessel are manufactured using a three-dimensional modeling apparatus. Therefore, it becomes possible to easily manufacture a small amount of various kinds of parts, and it becomes possible to provide a culture vessel corresponding to various uses. In particular, in the present embodiment, the culture vessel component is manufactured using ABS resin as a main material, and is coated with a coating material that does not affect culture. This makes it possible to manufacture parts with sufficient strength to withstand culture applications and without affecting culture.
In addition, according to the present embodiment, it is possible to easily and inexpensively manufacture small-lot, multi-variety culture container parts. As a result, it becomes possible to provide a single-use culture vessel on the premise of disposable, which has been difficult in the prior art, in an optimal shape adapted to the purpose of culture.
For example, when using the present embodiment, even if the shape of the culture vessel (the shape of the bottom surface 12 and the top plate 30) is determined in advance, various ancillary equipment can be attached to this standard culture vessel. become. Therefore, it becomes possible to provide a culture vessel adapted to the purpose of culture in a very short time.

DESCRIPTION OF SYMBOLS 10 ... culture tank 12 ... bottom face 14 ... side 16 ... support member 22 ... nozzle 23 ... porous body 24 ... part 26 ... coating material 28 ... film 30 ... top plate 32 ... culture medium supply nozzle 34 ... sensor 36 ... adapter 38 ... adapter 42 ... stirring blade 44 ... stirring shaft 100 ... culture vessel

Claims (3)

Forming a culture vessel part mainly composed of an ABS resin using a three-dimensional forming apparatus for forming a three-dimensional object by laminating heat-melted materials;
Applying a coating material that does not affect the target culture on the part;
Curing the coating material;
Attaching the part to a culture vessel;
A method of producing a culture vessel containing In the method for producing a culture vessel according to claim 1,
The step of attaching the component to the culture vessel comprises
Placing the component at the opening of the culture vessel;
Applying the coating material between the opening and the part;
A second curing step of curing the coating material;
A method of producing a culture vessel containing Forming a culture vessel part mainly composed of an ABS resin using a three-dimensional forming apparatus for forming a three-dimensional object by laminating heat-melted materials;
Applying a coating material that does not affect the target culture on the part;
Curing the coating material;
A method for producing a part for culture vessels, comprising

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