JP2019017266A - Cell storage vessel - Google Patents

Abstract

To provide a technology of avoiding situations in which culture medium and cell suspension stored in a storage vessel leak outside the storage vessel.SOLUTION: In a cell storage vessel according to one embodiment, a bottom wall part, a top wall part, and side wall parts define the interior space of a cell storage vessel. A supply/discharge part is provided in a side wall part and allows communication between the internal space and the cell storage vessel outside space. A ventilation part is provided on a side wall part near the bottom wall part and a partition part that extends from the bottom wall part to the top wall part defines an isolated region that covers the ventilation part forward of the ventilation part in the internal space, a first gap region is provided between the partition part and the top wall part that allows communication between the inside of the gap region in the internal space in the first gap region and the outside of the gap region.SELECTED DRAWING: Figure 1

Description

Embodiments described herein relate generally to a cell container that contains cells.

In regenerative medicine, tissues and organs are artificially created by cell culture. Patent Documents 1 and 2 disclose techniques for recovering cells cultured in a culture vessel. Patent Document 1 discloses a technique aimed at recovering cells detached from an adhesive surface. Patent Document 2 discloses a technique aiming to prevent leakage of a liquid material such as a culture solution in a state where a three-dimensional container is laid or when cells are cultured. . Patent Document 3 discloses a technique relating to a cell culture jig and an automatic medium exchange system for automating medium exchange work.

JP 2003-235541 A JP 2009-34078 A Japanese Patent Laying-Open No. 2015-84686

In these cell culture systems, a closed cell culture vessel is used in order to prevent external contamination of bacteria, viruses and the like. For example, in adhesive cell culture, when harvesting cells adhered to the bottom surface of the culture container, first the adhesion force between the cells and the bottom surface of the culture container is reduced using an enzyme, etc. A method of flushing with the culture solution and cell suspension is used. The culture medium, culture solution, and cell suspension washed away from the closed cell culture container can be stored in a closed system cell storage container that stores the culture medium, culture solution, and cell suspension. The supply / discharge of the culture medium, culture solution, and cell suspension to / from the storage container is realized by adjusting the atmospheric pressure in the storage container. The adjustment of the atmospheric pressure in the storage container is performed by supplying and exhausting gas in the storage container. Gas supply and exhaust in the storage container is performed through a vent provided in the storage container. The inside of the storage container communicates with the outside of the storage container (specifically, a pump for supplying and exhausting gas) through the vent. In this case, in a state where the culture medium, the culture solution, and the cell suspension are stored in the storage container, the stored culture medium, culture solution, and cell suspension leak to the outside of the storage container through the vent. There is a case. Therefore, there is a demand for a technique for avoiding a situation in which the culture medium, culture solution, and cell suspension stored in the storage container leak outside the storage container.

In one aspect, a cell container is provided. The cell storage container includes a bottom wall part, a ceiling wall part, a side wall part, a partition wall part, a supply / discharge part, and a ventilation part, and the bottom wall part, the ceiling wall part, and the side wall part define an internal space of the cell storage container. The bottom wall portion and the ceiling wall portion are spaced apart from each other via the side wall portion, the side wall portion extends from the bottom wall portion to the ceiling wall portion, and the supply / discharge portion is disposed on the side wall portion. Provided, communicates the internal space with the space outside the cell container, and is disposed on the bottom wall side, the surface of the bottom wall portion faces the internal space, and the inner surface of the supply / discharge portion The ventilation part is provided in the side wall part, communicates the internal space and the space outside the cell storage container, and the partition part extends from the bottom wall part to the ceiling wall part in the internal space. An isolation region is defined so as to cover the ventilation part in front of the part, and two end sides of the partition wall part extending from the bottom wall part toward the ceiling wall part are formed on the side wall part. The opening of the ventilation portion on the side of the isolation region is disposed between the two end sides of the partition wall portion, and a first gap region is provided between the partition wall portion and the ceiling wall portion, The first gap region communicates the inside of the isolation region and the outside of the isolation region in the internal space.

According to the above-described aspect, the internal space of the vent and the cell container (more specifically, via the isolation region defined by the partition wall and the gap region provided between the partition wall and the ceiling wall portion). Since the internal space communicates with the region excluding the isolation region), the air pressure in the internal space (more specifically, the region excluding the isolation region from the internal space) can be adjusted via the ventilation portion. By adjusting the atmospheric pressure of the internal space (more specifically, the region excluding the isolation region from the internal space), the culture medium, culture solution, cell for the internal space (more specifically, the region excluding the isolation region from the internal space) The supply and discharge of the suspension is performed well through the supply and discharge unit. Even when a culture medium, a culture solution, or a cell suspension is accommodated in the internal space (more specifically, the area excluding the isolation region from the internal space), the partition wall covers the opening of the aeration part. Inflow of the culture medium and cell suspension into the aeration part is sufficiently suppressed by the partition part.

In one embodiment, the cell container further includes a protrusion, and the protrusion extends from the ceiling wall to the bottom wall in the internal space, and extends along the partition wall outside the isolation region in the internal space. It extends and is provided so as to cover the first gap region, the protrusion and the partition wall form a labyrinth structure, and a second gap region is provided between the protrusion and the partition wall. Yes. In this way, the first gap region provided between the ceiling wall portion and the partition wall portion is covered by the protrusion, and further, the second gap region provided between the protrusion portion and the partition wall portion. Since the labyrinth structure is formed on the ceiling wall side, the medium, culture medium, and cell suspension are contained in the internal space (more specifically, the area excluding the isolated area from the internal space). In addition, the inflow of the medium, the culture solution, and the cell suspension into the aeration part can be more sufficiently suppressed.

In one embodiment, the surface of the bottom wall portion is inclined so as to approach the ceiling wall portion as it goes from the supply / discharge portion side to the side wall portion side. The surface of the bottom wall part which concerns on one Embodiment is divided into several surface area | regions, and each of several surface area is flat, and approaches a ceiling wall part as it goes to the side wall part side from the supply / discharge part side. So as to be inclined. Or the surface of the bottom wall part which concerns on one Embodiment is a curved surface. Thus, since the surface of the bottom wall portion is inclined as it goes from the supply / discharge portion side to the side wall portion side, the cell storage container is placed on a horizontal plane, and the bottom wall portion and the ceiling wall portion extend horizontally. When the medium, culture solution, and cell suspension stored in the internal space (more specifically, the region excluding the isolated region from the internal space) are discharged from the supply / discharge unit The medium, the culture medium, and the cell suspension can be discharged well from the supply / discharge section.

As described above, there is provided a technique for avoiding a situation in which a culture medium, a culture solution, or a cell suspension stored in a storage container leaks outside the storage container.

FIG. 1 is a front perspective view of a plane side according to the first embodiment. FIG. 2 is a planar rear perspective view according to the first embodiment. FIG. 3 is a right side view according to the first embodiment. FIG. 4 is a left side view according to the first embodiment. FIG. 5 is a plan view according to the first embodiment. FIG. 6 is a bottom view according to the first embodiment. FIG. 7 is a front view according to the first embodiment. FIG. 8 is a rear view according to the first embodiment. FIG. 9 is a bottom side rear perspective view according to the first embodiment. FIG. 10 is a bottom front perspective view according to the first embodiment. FIG. 11 is an enlarged CC cross-sectional view of DD and EE portions according to the first embodiment. FIG. 12 is an enlarged BB cross-sectional view of the DD portion according to the first embodiment. FIG. 13 is an AA cross-sectional view according to the first embodiment. FIG. 14 is a diagram illustrating the shape of the surface of the bottom wall portion on the internal space side according to the first embodiment. FIG. 15 is a diagram illustrating the configuration and arrangement of the partition wall according to the first embodiment. FIG. 16 is a front perspective view of the plane side according to the second embodiment. FIG. 17 is a planar rear perspective view according to the second embodiment. FIG. 18 is a right side view according to the second embodiment. FIG. 19 is a left side view according to the second embodiment. FIG. 20 is a plan view according to the second embodiment. FIG. 21 is a bottom view according to the second embodiment. FIG. 22 is a front view according to the second embodiment. FIG. 23 is a rear view according to the second embodiment. FIG. 24 is a bottom side rear perspective view according to the second embodiment. FIG. 25 is a bottom front perspective view according to the second embodiment. FIG. 26 is an enlarged CC cross-sectional view of DD and EE portions according to the second embodiment. FIG. 27 is an enlarged BB cross-sectional view of a DD portion according to the second embodiment. FIG. 28 is an AA cross-sectional view according to the second embodiment. FIG. 29 is a diagram illustrating the shape of the surface of the bottom wall portion on the side of the internal space according to the second embodiment. FIG. 30 is a reference bottom side rear perspective view according to the first embodiment. FIG. 31 is a reference enlarged CC cross-sectional view taken along DD and EE according to the first embodiment. FIG. 32 is a cross-sectional view taken along the line BB in the DD portion according to the first embodiment. FIG. 33 is a reference AA sectional view according to the first embodiment. FIG. 34 is a reference bottom side rear perspective view according to the second embodiment. FIG. 35 is a reference enlarged CC sectional view taken along the lines DD and EE according to the second embodiment. FIG. 36 is a cross-sectional view taken along the line BB in the DD portion according to the second embodiment. FIG. 37 is a reference AA sectional view according to the second embodiment.

Hereinafter, various embodiments will be described in detail with reference to the drawings. In the drawings, the same or corresponding parts are denoted by the same reference numerals.

(First embodiment)
The structure of the cell storage container 1 which concerns on 1st Embodiment is shown in FIGS. FIG. 1 is a front perspective view of a plane side according to the first embodiment. FIG. 2 is a planar rear perspective view according to the first embodiment. FIG. 3 is a right side view according to the first embodiment. FIG. 4 is a left side view according to the first embodiment. FIG. 5 is a plan view according to the first embodiment. FIG. 6 is a bottom view according to the first embodiment. FIG. 7 is a front view according to the first embodiment. FIG. 8 is a rear view according to the first embodiment. FIG. 9 is a bottom side rear perspective view according to the first embodiment. FIG. 10 is a bottom front perspective view according to the first embodiment. FIG. 11 is an enlarged CC cross-sectional view of DD and EE portions according to the first embodiment. FIG. 12 is an enlarged BB cross-sectional view of the DD portion according to the first embodiment. FIG. 13 is an AA cross-sectional view according to the first embodiment. FIG. 14 is a diagram illustrating the shape of the surface of the bottom wall portion on the internal space side according to the first embodiment. FIG. 15 is a diagram illustrating the configuration and arrangement of the partition wall according to the first embodiment.

The cell container 1 contains various adhesive (adhesive) cells such as pluripotent stem cells such as iPS cells and ES cells, chondrocytes such as bone marrow stromal cells (MSC), and dendritic cells. It can be used for the purpose of accommodating a medium, a culture solution, and a cell suspension used for culture. The cell container 1 may also be used as a medium for culturing floating cells such as blood cells, preferably T cells and B cells, a culture solution, a cell suspension, or a culture container for cell culture. Can be used. The cell storage container 1 includes a storage portion 1A and a frame portion 1B. In one embodiment, the material of the cell container 1 may include a highly transparent (transparent) resin such as polystyrene. When the cell container 1 has light transmittance, it becomes easy to optically observe the cells being cultured in the container 1A of the cell container 1 from the outside of the cell container 1. The cell container 1 can be integrally formed by, for example, injection molding.

The accommodating portion 1A includes a bottom wall portion 1A1, a ceiling wall portion 1A2, a side wall portion 1A3, a partition wall portion 1A4, a supply / discharge portion 1A5, and a ventilation portion 1A6. The accommodating portion 1A further includes a protruding portion 1A7. In the storage part 1A, the bottom wall part 1A1, the ceiling wall part 1A2, and the side wall part 1A3 define an internal space SP of the storage part 1A of the cell storage container 1. The internal space SP includes a main space SP1 and an isolation region ER. In the internal space SP, the main space SP1 and the isolation region ER are separated by a partition wall 1A4. In the internal space SP, the main space SP1 and the isolation region ER communicate with each other via a gap region VD1 (first gap region) and a gap region VD2 (second gap region) described later.

The bottom wall portion 1A1 and the ceiling wall portion 1A2 are provided apart from each other via the side wall portion 1A3. The bottom wall portion 1A1 and the ceiling wall portion 1A2 extend facing each other. The side wall 1A3 extends from the bottom wall 1A1 to the ceiling wall 1A2. The side wall portion 1A3 is provided along the edge of the bottom wall portion 1A1 and is provided along the edge of the ceiling wall portion 1A2. The side wall 1A3 extends from the edge of the bottom wall 1A1 to the edge of the ceiling wall 1A2. The bottom wall 1A1, the ceiling wall 1A2, and the side wall 1A3 define the shape of the box that forms the container 1A of the cell container 1.

The supply / discharge part 1A5 is provided on the side wall part 1A3. The supply / discharge portion 1A5 is disposed closer to the bottom wall portion 1A1 (on the side of the bottom wall portion). The supply / discharge unit 1A5 communicates the main space SP1 of the internal space SP and the space outside the storage unit 1A of the cell storage container 1. The surface 1A1f of the bottom wall portion 1A1 faces the internal space SP and is connected to the inner surface of the supply / discharge portion 1A5. In one embodiment, the surface 1A1f can be smoothly connected to the inner surface of the supply / discharge portion 1A5 without a step. The supply / discharge part 1A5 includes an opening 1A5a and an opening 1A5b. The inner surface of the supply / discharge part 1A5 extends from the opening 1A5a to the opening 1A5b. The opening 1A5a is on the side of the internal space SP and faces the main space SP1 of the internal space SP. The opening 1A5b is on the side of the space outside the accommodating portion 1A of the cell container 1 and faces this space. The supply / discharge part 1A5 is a flow path for liquids (hereinafter, simply referred to as liquids) such as a culture medium, a culture solution, and a cell suspension. A sealing member made of, for example, an elastic body (not shown) is inserted into the opening of the supply / discharge portion 1A5, and the supply and discharge of the liquid to the main space SP1 of the storage portion 1A can be performed airtightly via the sealing member. In a state where the cell storage container 1 is placed on a horizontal plane (in this case, the bottom wall portion 1A1 extends along the horizontal plane, and the surface 1A1f of the bottom wall portion 1A1 extends from the side of the side wall portion 1A3 to the supply / discharge portion 1A5. The liquid supplied from the outside of the cell storage container 1 via the supply / discharge part 1A5 is inclined to the opening 1A5a (inclined so as to be separated from the ceiling wall part 1A2). Furthermore, in a state where the opening 1A5b) is closed, it can be accommodated in the main space SP1.

The ventilation part 1A6 is provided in the side wall part 1A3. In one embodiment, the ventilation portion 1A6 can be disposed closer to the bottom wall portion 1A1. The ventilation portion 1A6 communicates the isolation region ER of the internal space SP with the space outside the storage portion 1A of the cell storage container 1. The ventilation portion 1A6 includes an opening 1A6a and an opening 1A6b. The inner surface of the ventilation portion 1A6 extends from the opening 1A6a to the opening 1A6b. The opening 1A6a is on the inner space SP side and faces the isolation region ER of the inner space SP. The opening 1 </ b> A <b> 6 b is on the side of the space outside the housing portion 1 </ b> A of the cell housing container 1 and faces this space. The ventilation portion 1A6 is a flow passage for gas (which is a gas used for adjusting the internal pressure of the main space SP1, and may be simply referred to as gas below). Gas can be supplied to and discharged from the main space SP1 of the housing portion 1A through the ventilation portion 1A6.

In the internal space SP of the accommodating portion 1A, an opening 1A5a (further opening 1A5b) of the supply / discharge portion 1A5 and an opening 1A6a (further opening 1A6b) of the ventilation portion 1A6 are inserted with a sealing member made of, for example, an elastic body (not shown). Airtightness is maintained in the closed state. Access from the outside of the cell storage container 1 to the internal space SP of the storage portion 1A can be performed while the airtight state is maintained by inserting a needle-like connection member into the sealing member.

The partition wall portion 1A4 extends from the bottom wall portion 1A1 toward the ceiling wall portion 1A2 in the internal space SP (for example, see particularly FIG. 15). The partition wall 1A4 is joined to the bottom wall 1A1. In the internal space SP, the partition wall 1A4 is disposed in front of the ventilation part 1A6 so as to cover the ventilation part 1A6 (more specifically, so as to cover the opening 1A6a of the ventilation part 1A6). Are defined. The partition wall portion 1A4 is joined to the bottom wall portion 1A1 and the side wall portion 1A3 so as to define the main space SP1 and the isolation region ER. The partition wall portion 1A4 includes an end side portion 1A4a, an end side portion 1A4b, an end side portion 1A4c, and an end side portion 1A4d. As described above, the partition wall 1A4 has a plate shape having four sides in a plan view. The partition wall 1A4 has a surface 1A4f. The surface 1A4f is defined by an end side portion 1A4a, an end side portion 1A4b, an end side portion 1A4c, and an end side portion 1A4d. The surface 1A4f faces the main space SP1 in the internal space SP. The back surface of the partition wall 1A4 with respect to the front surface 1A4f faces the isolation region ER in the internal space SP.

The direction in which the end side portion 1A4a and the end side portion 1A4b extend and the direction in which the end side portion 1A4c and the end side portion 1A4d extend intersect each other. The end side portion 1A4a and the end side portion 1A4b extend side by side from the end side portion 1A4c to the end side portion 1A4d. The edge 1A4a is joined to the bottom wall 1A1, and is along the surface 1A1f of the bottom wall 1A1 (surface 1A1f that is inclined with respect to the horizontal plane when the cell container 1 is placed on the horizontal plane). Along). The end side portion 1A4b faces the ceiling wall portion 1A2 and extends along the ceiling wall portion 1A2 at a position close to the ceiling wall portion 1A2. The end side portion 1A4c and the end side portion 1A4d extend side by side from the end side portion 1A4a to the end side portion 1A4b. One end of the end side portion 1A4c and one end of the end side portion 1A4d are both joined to the bottom wall portion 1A1. The end side portion 1A4c and the end side portion 1A4d extend from the bottom wall portion 1A1 toward the ceiling wall portion 1A2, and are joined to the side wall portion 1A3.

In the side wall portion 1A3, an opening 1A6a of the ventilation portion 1A6 on the inner space SP side is disposed between the end side portion 1A4c and the end side portion 1A4d. A gap region VD1 is provided between the partition wall 1A4 and the ceiling wall 1A2. The gap region VD1 is disposed between the end portion 1A4b of the partition wall portion 1A4 and the ceiling wall portion 1A2. The gap region VD1 communicates the inside of the isolation region ER and the outside of the isolation region ER (main space SP1) in the internal space SP.

The protrusion 1A7 extends from the ceiling wall 1A2 toward the bottom wall 1A1 in the internal space SP (specifically, the main space SP1). The protrusion 1A7 extends along the partition wall 1A4 (more specifically, the surface 1A4f of the partition wall 1A4) outside the isolation region ER in the internal space SP (on the main space SP1 side). The protrusion 1A7 is provided so as to cover the gap region VD1 in the internal space SP (specifically, the main space SP1). The protrusion 1A7 and the partition wall 1A4 form a labyrinth structure. A gap region VD2 is provided between the protrusion 1A7 and the partition wall 1A4.

The gas supplied from the outside of the cell storage container 1 to the internal space SP (specifically, the main space SP1) of the storage portion 1A through the ventilation portion 1A6 is supplied from the opening 1A6b of the ventilation portion 1A6 to the opening 1A6a and the isolation region ER. The main space SP1 is reached through the gap region VD1 and the gap region VD2 in this order. On the other hand, in the labyrinth structure provided between the protrusion 1A7 and the partition wall 1A4, the liquid accommodated in the main space SP1 enters the isolation region ER via the gap region VD2 and reaches the ventilation portion 1A6. Avoid the situation. By this labyrinth structure, when supplying a liquid such as a culture medium to the main space SP1 of the storage portion 1A, or when transporting the cell storage container 1 in a state where the main space SP1 is filled with a liquid such as a culture medium, the isolation region ER The liquid can be effectively prevented from entering.

In the state where the cell container 1 is placed on the horizontal plane, the supply and discharge of the liquid to and from the main space SP1 via the supply / discharge portion 1A5 are performed via the ventilation portion 1A6 without the liquid entering the ventilation portion 1A6. This can be performed by increasing or decreasing (adjusting) the atmospheric pressure in the internal space SP by supplying and discharging gas.

The configuration of the bottom wall portion 1A1 will be described in more detail. The bottom wall portion 1A1 may have a substantially uniform thickness in one embodiment. Accordingly, the back surface of the bottom wall portion 1A1 with respect to the front surface 1A1f is substantially parallel to the front surface 1A1f (in the present specification, “substantially parallel” indicates that the geometrically strict meaning is not necessarily limited to parallel). The same). The back surface is inclined in the same manner as the front surface 1A1f. The surface 1A1f of the bottom wall portion 1A1 is inclined so as to approach the ceiling wall portion 1A2 from the opening 1A5a side of the supply / discharge portion 1A5 toward the side wall portion 1A3. In a state where the cell container 1 is placed on a horizontal plane, the surface 1A1f is lowered from the side wall 1A3 side toward the opening 1A5a side of the supply / discharge portion 1A5 (so as to be separated from the ceiling wall 1A2). To). In a state where the cell container 1 is placed on a horizontal plane, the ceiling wall portion 1A2 extends horizontally, and the surface 1A1f of the bottom wall portion 1A1 has a horizontal plane (with respect to the direction in which the ceiling wall portion 1A2 extends). And slanted. For example, when the sphere is placed on the surface 1A1f at any position on the surface 1A1f in a state where the cell container 1 is placed on a horizontal plane, the sphere rolls on the surface 1A1f by gravity. It is provided so as to reach the opening 1A5a of the discharge portion 1A5. Accordingly, the cell storage container 1 is placed on a horizontal plane, the opening 1A5a of the supply / discharge section 1A5 is closed, and the liquid is stored in the main space SP1, and the opening 1A5a is opened to be stored in the main space SP1. When the discharged liquid is discharged to the outside through the supply / discharge portion 1A5, the surface 1A1f of the bottom wall portion 1A1 is inclined as described above. It can flow on the surface 1A1f, be efficiently collected in the supply / discharge section 1A5, and discharged from the supply / discharge section 1A5 to the outside.

The surface 1A1f of the bottom wall portion 1A1 is divided into a plurality of surface regions, and each of the plurality of surface regions is flat, and the ceiling wall portion is directed toward the side wall portion 1A3 from the supply / discharge portion 1A5 side. It can incline so that it may approach 1A2. In the first embodiment, specifically, as shown in FIGS. 6, 9, 10, 13, and 14, the surface 1A1f of the bottom wall portion 1A1 has two surface regions (surface region PE1a, surface It can be divided into areas PE1b). The surface 1A1f includes a surface region PE1a and a surface region PE1b. Each of the surface region PE1a and the surface region PE1b is flat and can be inclined so as to approach the ceiling wall portion 1A2 from the supply / discharge portion 1A5 side toward the side wall portion 1A3.

The frame portion 1B includes a bottom portion 1B1 and a wall portion 1B2. The accommodating portion 1A is provided on the bottom portion 1B1. The bottom 1B1 has a plate shape. The accommodating portion 1A is disposed at substantially the center of the bottom portion 1B1. The wall 1B2 is provided along the outer edge of the bottom 1B1. The wall 1B2 extends substantially parallel to the side wall 1A3 along the side wall 1A3 of the housing 1A. The opening 1A5b of the supply / discharge portion 1A5 and the opening 1A6b of the ventilation portion 1A6 are provided in the wall portion 1B2.

According to the cell container 1 according to the embodiment described above, the ventilation is performed via the isolation region ER defined by the partition wall portion 1A4 and the gap region VD1 provided between the partition wall portion 1A4 and the ceiling wall portion 1A2. Since the portion 1A6 communicates with the main space SP1 of the internal space SP, the atmospheric pressure of the main space SP1 of the internal space SP can be adjusted via the ventilation portion 1A6. By adjusting the atmospheric pressure of the main space SP1, liquid supply / discharge of the main space SP1 is favorably performed through the supply / discharge section 1A5. Even when the liquid is stored in the main space SP1, since the partition wall 1A4 covers the opening 1A6a of the ventilation portion 1A6, the flow of the liquid stored in the main space SP1 into the ventilation portion 1A6 is caused by the partition wall 1A4. Sufficiently suppressed.

Further, the gap portion VD1 provided between the ceiling wall portion 1A2 and the partition wall portion 1A4 is covered by the protrusion 1A7, and further, the gap region VD2 provided between the protrusion portion 1A7 and the partition wall portion 1A4. Since the labyrinth structure is formed on the ceiling wall portion 1A2 side, even when the liquid is stored in the main space SP1 of the internal space SP, the inflow of the liquid into the ventilation portion 1A6 can be sufficiently suppressed. In this case, if the surface (liquid level) of the liquid stored in the main space SP1 is located at a lower level than the surface of the protrusion 1A7, the liquid overflows from the partition wall 1A4 and enters the ventilation part 1A6. There is no. Further, when the cell storage container 1 in a state where the main space SP1 is filled with the liquid is transported, the liquid can be effectively prevented from entering the isolation region ER.

Further, since the surface 1A1f of the bottom wall portion 1A1 is inclined so as to approach the ceiling wall portion 1A2 from the opening 1A5a side of the supply / discharge portion 1A5 toward the side wall portion 1A3, the cell container 1 is placed on a horizontal plane. When the liquid stored in the main space SP1 of the internal space SP is discharged from the supply / discharge portion 1A5 in a state where the bottom wall portion 1A1 and the ceiling wall portion 1A2 are horizontally extended, the liquid is supplied / discharged portion 1A5. Can be discharged well.

(Second Embodiment)
The structure of the cell storage container 1 which concerns on 2nd Embodiment is shown in FIGS. FIG. 16 is a front perspective view of the plane side according to the second embodiment. FIG. 17 is a planar rear perspective view according to the second embodiment. FIG. 18 is a right side view according to the second embodiment. FIG. 19 is a left side view according to the second embodiment. FIG. 20 is a plan view according to the second embodiment. FIG. 21 is a bottom view according to the second embodiment. FIG. 22 is a front view according to the second embodiment. FIG. 23 is a rear view according to the second embodiment. FIG. 24 is a bottom side rear perspective view according to the second embodiment. FIG. 25 is a bottom front perspective view according to the second embodiment. FIG. 26 is an enlarged CC cross-sectional view of DD and EE portions according to the second embodiment. FIG. 27 is an enlarged BB cross-sectional view of a DD portion according to the second embodiment. FIG. 28 is an AA cross-sectional view according to the second embodiment. FIG. 29 is a diagram illustrating the shape of the surface of the bottom wall portion on the side of the internal space according to the second embodiment.

The difference between the second embodiment and the first embodiment is only the difference in the configuration of the bottom wall portion 1A1 (the configuration of the surface 1A1f), and the others are the same as those in the first embodiment and the second embodiment. Have the same configuration. In the second embodiment, as shown in FIGS. 21, 24, 25, 28, and 29, the surface 1A1f of the bottom wall 1A1 has three surface regions (surface region PE2a, surface region PE2b, surface region). PE2c). In the second embodiment, the surface 1A1f includes a surface region PE2a, a surface region PE2b, and a surface region PE2c. Each of the surface region PE2a, the surface region PE2b, and the surface region PE2c is flat and can be inclined so as to approach the ceiling wall portion 1A2 from the supply / discharge portion 1A5 side toward the side wall portion 1A3. The surface 1A1f can be divided into four or more surface regions. By partitioning the surface 1A1f into multiple surfaces, a large liquid storage volume in the main space SP1 can be secured. It should be noted that the region of the surface 1A1f of the bottom wall 1A1 that is farthest from the ceiling wall 1A2 (the lowest region of the surface 1A1f of the bottom wall 1A1 when the cell container 1 is laid flat) and the supply / discharge The part 1A5 only needs to be connected, and is not limited to the illustrated embodiment, and can be modified. The surface 1A1f can be a curved surface. In this case, the curved surface is inclined so as to approach the ceiling wall portion 1A2 as it goes from the supply / discharge portion 1A5 side to the side wall portion 1A3 side. The configuration shown in FIG. 15 is also applied to the second embodiment.

With reference to FIGS. 30, 31, 32, and 33, the first embodiment will be generally described. FIG. 30 is a reference bottom side rear perspective view according to the first embodiment. FIG. 31 is a reference enlarged CC cross-sectional view taken along DD and EE according to the first embodiment. FIG. 32 is a cross-sectional view taken along the line BB in the DD portion according to the first embodiment. FIG. 33 is a reference AA sectional view according to the first embodiment. With reference to FIG. 34, FIG. 35, FIG. 36, and FIG. 37, the second embodiment will be generally described. FIG. 34 is a reference bottom side rear perspective view according to the second embodiment. FIG. 35 is a reference enlarged CC sectional view taken along the lines DD and EE according to the second embodiment. FIG. 36 is a cross-sectional view taken along the line BB in the DD portion according to the second embodiment. FIG. 37 is a reference AA sectional view according to the second embodiment.

Description of the article according to the design: This article (the cell storage container 1 according to one embodiment and the same applies hereinafter) is a cell storage container for recovering cells cultured in the cell culture container. In the present article, supply and discharge of the culture medium, the culture solution, and the cell suspension are performed through the supply and discharge unit by adjusting the atmospheric pressure by the ventilation unit. In this product, not only the partition walls but also the projections on the ceiling wall make the gaps smaller and maintain the function of adjusting the air pressure of the ventilation part, while allowing ventilation of the culture medium, culture solution, and cell suspension. Inflow to the part can be suppressed. In the present article, the upper surface portion of the bottom wall portion is divided into a plurality of surface regions, and is inclined downward from the back side wall portion toward the supply / discharge portion side. The article can discharge the contained culture medium, culture solution, and cell suspension from the supply / discharge section in a state where the article is placed on a horizontal plane by the inclination of the upper surface of the bottom wall.

Description of the design: The product is entirely transparent.

While the principles of the invention have been illustrated and described in the preferred embodiments, it will be appreciated by those skilled in the art that the invention can be modified in arrangement and detail without departing from such principles. The present invention is not limited to the specific configuration disclosed in the present embodiment. We therefore claim all modifications and changes that come within the scope and spirit of the following claims.

DESCRIPTION OF SYMBOLS 1 ... Cell accommodation container, 1A ... Accommodating part, 1A1 ... Bottom wall part, 1A1f ... Surface, 1A2 ... Ceiling wall part, 1A3 ... Side wall part, 1A4 ... Partition wall part, 1A4a ... End side part, 1A4b ... End side part, 1A4c ... end side part, 1A4d ... end side part, 1A4f ... surface, 1A5 ... supply / discharge part, 1A5a ... opening, 1A5b ... opening, 1A6 ... venting part, 1A6a ... opening, 1A6b ... opening, 1A7 ... projecting part, 1B ... frame Part, 1B1 ... bottom part, 1B2 ... wall part, ER ... isolation region, PE1a ... surface region, PE1b ... surface region, PE2a ... surface region, PE2b ... surface region, PE2c ... surface region, SP ... internal space, SP1 ... main space , VD1 ... gap area, VD2 ... gap area.

Claims (5)

A cell container,
A bottom wall portion, a ceiling wall portion, a side wall portion, a partition wall portion, a supply / discharge portion, and a ventilation portion are provided,
The bottom wall portion, the ceiling wall portion, and the side wall portion define an internal space of the cell container;
The bottom wall portion and the ceiling wall portion are provided apart from each other through the side wall portion,
The side wall portion extends from the bottom wall portion to the ceiling wall portion,
The supply / discharge portion is provided on the side wall portion, communicates the internal space with the space outside the cell storage container, and is disposed on the bottom wall portion side,
The surface of the bottom wall portion faces the internal space, and is connected to the inner surface of the supply / discharge portion,
The vent is provided in the side wall, and communicates the internal space with the space outside the cell container,
The partition portion extends from the bottom wall portion toward the ceiling wall portion in the internal space, and defines an isolation region so as to cover the ventilation portion in front of the ventilation portion,
Two end sides of the partition wall extending from the bottom wall toward the ceiling wall are joined to the side wall,
Between the two end sides of the partition wall, an opening of the ventilation portion on the side of the isolation region is disposed,
A first gap region is provided between the partition wall and the ceiling wall.
The first gap region communicates the inside of the isolation region and the outside of the isolation region in the internal space.
Cell container. It further includes a protrusion,
The protrusion extends from the ceiling wall portion toward the bottom wall portion in the internal space, extends along the partition wall outside the isolation region in the internal space, and a first gap region Is provided to cover
The protrusion and the partition wall form a labyrinth structure,
A second gap region is provided between the protrusion and the partition.
The cell container according to claim 1. The surface of the bottom wall portion is inclined so as to approach the ceiling wall portion from the supply / discharge portion side toward the side wall portion.
The cell container according to claim 1 or 2. The surface of the bottom wall is divided into a plurality of surface regions;
Each of the plurality of surface regions is flat and is inclined so as to approach the ceiling wall portion from the supply / discharge portion side toward the side wall portion.
The cell container according to claim 3. The surface of the bottom wall is a curved surface;
The cell container according to claim 3.

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