JP2019013191A - Cultured cell analysis system, cultured cell analysis method, and program - Google Patents

Abstract

To correctly analyze an adherent cell.SOLUTION: A cultured cell analysis system 1 comprises: an image data generation device 20 which images a biological sample including an adherent cell and generates image data; a determination part 40b; and an analysis part 40d. The determination part 40b determines whether or not a state of adherent cell is an adhesion state or a floating state. The analysis part 40d analyzes data to be analyzed which is image data generated by the image data generation device 20 and image data of the biological sample in the adhesion state specified by the determination part 40b based on a determination result.SELECTED DRAWING: Figure 3

Description

  The disclosure of the present specification relates to a cultured cell analysis system, a cultured cell analysis method, and a program.

  In the biochemical field, an analysis system is known that repeatedly captures images of cultured cells in a controlled environment and analyzes the obtained data. Such an analysis system is described in Patent Document 1, for example. Patent Document 1 describes storing culture environment information, analysis condition information, and analysis result information in association with each other.

JP 2007-20422 A

  By the way, adherent cells (also referred to as adherent cells) that grow by adhering to a culture vessel are a type of cultured cells. In the culture of adherent cells, when passage (also referred to as planting) of transferring adherent cells to a container containing a new medium is performed, the adherent cells float in the container until they adhere to the new container. It becomes. If an analysis is performed using an image acquired in this state, the number of adherent cells is not counted correctly, and the analysis accuracy deteriorates.

  Based on the above situation, an object according to one aspect of the present invention is to provide a technique for correctly analyzing adherent cells.

  The cultured cell analysis system according to one aspect of the present invention includes an image data generation device that captures an image of a biological sample including adherent cells and generates image data, and a determination unit that determines whether the state of the adherent cells is an adhesive state or a floating state And an analysis unit for analyzing analysis target data, which is image data generated by the image data generation device and is image data of the biological sample in the adhesion state specified based on a determination result by the determination unit .

  In the cultured cell analysis method according to one aspect of the present invention, a biological sample including adherent cells is imaged to generate image data, whether the state of the adherent cells is an adherent state or a floating state, and the generated image data is used. Then, analysis target data, which is image data of the biological sample in the attached state specified based on the determination result about the state of the adherent cell, is analyzed.

  The program according to one aspect of the present invention is a computer that acquires image data of a biological sample containing adherent cells, determines whether the state of the adherent cells is an adherent state or a floating state, and is obtained image data, A process of analyzing analysis target data, which is image data of the biological sample in the attached state specified based on a determination result on the state of the adherent cell, is executed.

  According to said aspect, the technique which analyzes an adherent cell correctly can be provided.

It is the figure which illustrated the composition of cultured cell analysis system 1 concerning a 1st embodiment. 2 is a diagram illustrating a hardware configuration of a server 40. FIG. 3 is an example of a functional block diagram of a server 40. FIG. It is a flowchart which shows an example of the cultured cell analysis process which concerns on 1st Embodiment. It is a flowchart which shows an example of a time lapse control process. It is an example of an input screen. It is an example of a display of an analysis result. It is a display example of a conventional analysis result. It is a flowchart which shows an example of the cultured cell analysis process which concerns on 2nd Embodiment. It is another example of an input screen. 3 is an example of a functional block diagram of a server 90. FIG. It is a flowchart which shows an example of the cultured cell analysis process which concerns on 3rd Embodiment.

[First Embodiment]
FIG. 1 is a diagram illustrating the configuration of a cultured cell analysis system 1 according to this embodiment. FIG. 2 is a diagram illustrating a hardware configuration of the server 40. The cultured cell analysis system 1 is a system for imaging a biological sample accommodated in the microplate 30 and analyzing the obtained data. The target biological sample is a biological sample containing adherent cells.

  The cultured cell analysis system 1 includes an image data generation device 20 that images a biological sample and generates image data, and a server 40 that performs analysis processing based on at least the image data generated by the image data generation device 20. Yes. The image data generation device 20 and the server 40 are connected by a wired cable such as a USB (Universal Serial Bus) cable. The image data generation device 20 and the server 40 may be configured to be able to exchange data with each other, and may be connected to be communicable wirelessly, not limited to wired communication.

  The cultured cell analysis system 1 may further include a liquid crystal display 50 that is a display device that displays an analysis result, and a keyboard 60 that is an input device that inputs imaging conditions. The analysis result includes, for example, the number and density of adherent cells. The imaging conditions are conditions for imaging control processing for time-lapse observation (hereinafter referred to as time-lapse control processing). For example, the image data size and the waiting time until imaging start (hereinafter referred to as start delay time). ), An imaging time interval (hereinafter also referred to as a scan interval), an imaging count (hereinafter also referred to as a scan count), and the like.

  Note that the user of the cultured cell analysis system 1 may access the cultured cell analysis system 1 through at least one of wireless or wired communication using a client terminal such as a notebook computer 70, a tablet computer 80, or a smartphone. In this case, the client terminal is a display device that displays the analysis result, and an input device that inputs imaging conditions.

  For example, as shown in FIG. 1, the image data generation device 20 is installed in an incubator 10 in which a microplate 30 is arranged. The microplate 30 has a plurality of wells 31, each of which is a storage unit, and a biological sample is stored in each well 31. The incubator 10 is a device that maintains or controls the culture environment, and here, is an incubator for culturing a biological sample accommodated in the microplate 30.

  FIG. 1 shows an example in which the microplate 30 is placed on the support plate in the incubator 10 so as to be spaced apart from the image data generation device 20, but the microplate 30 is a support plate. It may be placed directly on the upper surface of the image data generation device 20 without going through. Moreover, the culture container for the biological sample is not limited to the microplate 30 and may be a flask, a dish, or the like, for example.

  The image data generation device 20 includes an image sensor 22, a plurality of illumination LED light sources 23 arranged around the image sensor 22, and a temperature sensor 24. The image sensor 22 is, for example, a CCD (Charge-Coupled Device) image sensor, a CMOS (Complementary MOS) image sensor, or the like. The image sensor 22 and the plurality of illumination LED light sources 23 are provided so as to be movable under the imaging region 21. The temperature sensor 24 is a sensor that measures the temperature in the incubator 10. Note that the image data generation device 20 may include other sensors that measure the environment in the incubator 10.

  The server 40 is, for example, a standard computer. As shown in FIG. 2, the server 40 includes a processor 41, a memory 42, a storage 43, an interface device 44, and a portable storage medium driving device 45 into which a portable storage medium 46 is inserted. Are connected to each other.

  The processor 41 is, for example, a central processing unit (CPU), a micro processing unit (MPU), a digital signal processor (DSP), and the like, and executes a programmed process by executing a program. The memory 42 is, for example, a RAM (Random Access Memory), and temporarily stores a program or data stored in the storage 43 or the portable storage medium 46 when the program is executed.

  The storage 43 is, for example, a hard disk or a flash memory, and is mainly used for storing various data and programs. The interface device 44 is a circuit that exchanges signals with devices other than the server 40 (for example, the image data generation device 20, the liquid crystal display 50, the keyboard 60, the notebook computer 70, the tablet computer 80, and the like).

  The portable storage medium driving device 45 accommodates a portable storage medium 46 such as an optical disk or a compact flash (registered trademark). The portable storage medium 46 has a role of assisting the storage 43. The storage 43 and the portable storage medium 46 are examples of non-transitory computer-readable storage media each storing a program.

  The configuration illustrated in FIG. 2 is an example of the hardware configuration of the server 40, and the server 40 is not limited to this configuration. The server 40 may be a dedicated device instead of a general-purpose device. The server 40 may include an electrical circuit such as an application specific integrated circuit (ASIC) or a field programmable gate array (FPGA) instead of or in addition to the processor that executes the program. All or part of the above may be performed.

  FIG. 3 is an example of a functional block diagram of the server 40. The server 40 includes an imaging condition acquisition unit 40a, a determination unit 40b, an imaging control unit 40c, an analysis unit 40d, a display control unit 40e, an imaging condition storage unit 40f, a time-lapse image storage unit 40g, and an analysis result storage unit 40h. Yes.

  The imaging condition acquisition unit 40a acquires imaging conditions input by the user using the keyboard 60 that is an input device, and stores the acquired imaging conditions in the imaging condition storage unit 40f. The imaging condition acquisition unit 40a may acquire, for example, the image data size, the start delay time, the scan interval, and the number of scans as the imaging conditions, and may store these in the imaging condition storage unit 40f.

  The determination unit 40b determines whether the state of the adherent cells contained in the biological sample accommodated in the well 31 is an adhesion state or a floating state. The adherent state refers to the state where adherent cells adhere to the container surface, etc., and the floating state refers to the state where adherent cells do not adhere to the container surface etc. and are suspended in the culture medium. Say. Specifically, the determination unit 40b may determine the state of adherent cells based on the elapsed time from the reference time. In this case, it is desirable to store in advance in the imaging condition storage unit 40f as the start delay time the time required from the passage to the time when the adherent cells are in an adhesive state. Thereby, the determination unit 40b can determine the state of the adherent cell by comparing the elapsed time from the reference time (for example, the passage time) with the start delay time.

  The imaging control unit 40c controls the image data generation device 20 according to the imaging conditions stored in the imaging condition storage unit 40f. Specifically, when the determination unit 40b determines that the state of the adherent cell is an adhesive state, the imaging control unit 40c starts the time lapse control process and images the biological sample at a preset time interval. The data generation device 20 may be controlled. In this case, it is desirable to store the preset time interval in advance in the imaging condition storage unit 40f as a scanning interval as an imaging condition. Thereby, the imaging control unit 40c may read the scan interval from the imaging condition storage unit 40f and control the image data generation device 20 so as to image the biological sample at the read scan interval.

  The analysis unit 40d analyzes the analysis target data and stores it in the analysis result storage unit 40h. The analysis target data is image data generated by the image data generation device 20, and is image data of a biological sample in an adhesive state that is specified based on a determination result by the determination unit 40b. Note that the image data of the adhered biological sample is image data generated by imaging the adhered cells in the adhered state. Specifically, the analysis unit 40d may specify the image data generated by the image data generation device 20 after the determination unit 40b determines that the state of the adherent cells is an adhesion state as analysis target data. The analysis process performed by the analysis unit 40d is not particularly limited, and may include, for example, a process of counting the number of adherent cells, a process of calculating the density of adherent cells, and the like.

  The display control unit 40e displays the analysis result by the analysis unit 40d on the liquid crystal display 50 that is a display device. The display control unit 40e may display, for example, the cell number and density of the adherent cells on the liquid crystal display 50, and may display these temporal changes in a graph format.

  The imaging condition storage unit 40f stores the imaging conditions acquired by the imaging condition acquisition unit 40a. The time-lapse image storage unit 40g stores the image data generated by the image data generation device 20 after the determination unit 40b determines that the state of the adherent cells is an adhesive state. The analysis result storage unit 40h stores the analysis result obtained by the analysis unit 40d.

  In the server 40, the processor 41 executes programs loaded in the memory 42, thereby realizing various functions shown in FIG. However, the various functional units illustrated in FIG. 3 are not limited to those realized using software, and may be configured by dedicated circuits, for example.

  In the cultured cell analysis system 1 configured as described above, the determination unit 40b determines the state of the adherent cell, and the analysis unit uses the image data specified as the image data of the biological sample in the adherence state as an analysis target. 40d can perform analysis processing. Therefore, according to the cultured cell analysis system 1, it is possible to avoid analysis inconvenience caused by the adherent cells being in a floating state, and the adherent cells can be analyzed correctly.

  FIG. 4 is a flowchart showing an example of the cultured cell analysis process according to the present embodiment. FIG. 5 is a flowchart illustrating an example of the time lapse control process. FIG. 6 is an example of an input screen. FIG. 7 is a display example of the analysis result. FIG. 8 is a display example of a conventional analysis result. Hereinafter, the cultured cell analysis process performed in the cultured cell analysis system 1 will be specifically described with reference to FIGS. 4 to 8.

  When the cultured cell analysis process shown in FIG. 4 is started, first, the server 40 acquires imaging conditions (step S10). Here, the server 40 displays the input screen 100 shown in FIG. 6 on the liquid crystal display 50, for example. When the user inputs an imaging condition on the input screen 100 using the keyboard 60, the server 40 acquires the input imaging condition and stores it in the imaging condition storage unit 40f that is the memory 42 or the storage 43. In FIG. 6, the image data size is manipulated by operating the drop-down list 101, the start delay time is manipulated by operating the drop-down list 102, and the scan interval is manipulated by operating the drop-down list 103. An example in which the number of scans is selected by operating the list 104 is shown.

  Next, the server 40 determines the state of adherent cells (step S20). Here, the server 40 determines the state of the adherent cell based on whether the start delay time acquired in step S10 has elapsed since the start of the cultured cell analysis process. The server 40 determines that the state of the adherent cell is in an adhesive state when the start delay time has elapsed from the start of the cultured cell analysis process, and determines that the state of the adherent cell is in a floating state when it has not elapsed.

  When the server 40 determines that the state of the adherent cell is not the adherent state (NO in step S30), the server 40 waits for a certain period of time (step S40), and repeats the processes of step S20 and step S30. On the other hand, when the server 40 determines that the state of the adherent cell is the adherent state (step S30 YES), the server 40 performs the time lapse control process shown in FIG. 5 (step S50).

  In the time lapse control process, the server 40 first determines whether or not the current time has reached the imaging time (step S51). Here, the server 40 calculates the imaging time based on the scan interval acquired in step S10. If it is determined that the current time has reached the imaging time, the server 40 outputs an imaging instruction to the image data generation device 20 (step S52). Thereafter, the server 40 receives the image data from the image data generation device 20 (step S53), and stores the image data as analysis target data in the time-lapse image storage unit 40g which is the storage 43 (step S54). Finally, the server 40 determines whether or not to end the time lapse control process (step S55). Here, the server 40 determines not to end the time lapse control process when the number of repetitions of the process from step S51 to step S55 has not reached the number of scans acquired in step S10, and performs the process from step S51 to step S55. repeat. on the other hand. The server 40 ends the time lapse control process when the number of repetitions reaches the number of scans.

  When the time lapse control process ends, the server 40 analyzes the image data (step S60). Here, the server 40 reads image data from the time-lapse image storage unit 40g, and analyzes the read image data as analysis target data. The server 40 further stores the analysis result in the analysis result storage unit 40 h that is the storage 43.

  Finally, the server 40 displays the analysis result (step S70). Here, the server 40 reads the analysis result from the analysis result storage unit 40 h and displays the read analysis result on the liquid crystal display 50. The graph G1 shown in FIG. 7 is an example of the analysis result displayed on the liquid crystal display 50 in step S70, and shows the time change of the number of adherent cells. Peaks P1 and P2 in the graph indicate the passage timing. A graph G2 shown in FIG. 8 is an example of an analysis result in the conventional analysis system shown as a comparative example.

  When the conventional analysis system and the cultured cell analysis system 1 are compared, there are the following differences. In a conventional analysis system, analysis processing is performed without determining the state of adherent cells. For this reason, as shown in FIG. 8, the liquid crystal display 50 displays the analysis results for both the floating period and the adhesion period. The analysis result in the floating period is inferior to the analysis result in the adhesion period in terms of analysis accuracy. For this reason, the accuracy as a whole of the analysis result obtained by the conventional analysis system is not necessarily high.

  In contrast, the cultured cell analysis system 1 determines the state of adherent cells and analyzes image data of adherent cells in an adherent state. For this reason, as shown in FIG. 7, only the analysis result of the period in the floating state is displayed on the liquid crystal display 50. In the adhered state, various analyzes such as counting of the number of cells can be performed with high accuracy. For this reason, according to the cultured cell analysis system 1, it is possible to provide the user with an analysis result with higher accuracy than the conventional analysis system.

  Further, if the purpose of analysis of adherent cells is to monitor the growth state of adherent cells, the analysis result of adherent cells in the floating state is less important than the analysis result of adherent cells in the adherent state. This is because adherent cells are cells that grow in an adherent state. For this reason, the cultured cell analysis system 1 that analyzes the image data of the adherent cells that are in the floating state, which is relatively less important, and that analyzes the image data of the adherent cells that are in the more important state is analyzed efficiency. This is also superior to conventional analysis systems.

  In the cultured cell analysis system 1, the time lapse control process is started after it is determined that the state of the adherent cell is the adherent state. For this reason, the time-lapse image stored in the storage 43 is an image in an adhesive state. Thereby, the work amount of the user who confirms the growth state of the adherent cells with reference to the time-lapse image can be suppressed. Therefore, the cultured cell analysis system 1 can improve the work efficiency of the user compared to the conventional analysis system.

[Second Embodiment]
The cultured cell analysis system according to this embodiment is different from the cultured cell analysis system 1 in that the determination method of the state of the adherent cell in the determination unit 40b is different. In the present embodiment, the determination unit 40b determines the state of adherent cells based on the image data generated by the image data generation device 20.

  More specifically, for example, the determination unit 40b may calculate the contrast of the image of the biological sample from the image data and determine the state of the adherent cell based on the calculated contrast. The image data generation device 20 is used in a state where the focus position is adjusted in advance to the adhesion surface (for example, the bottom surface of the well 31) so that the adherent cells in the adhesion state can be observed well. For this reason, when the adherent cells are in an adhesive state, the contrast of the image is high, and when they are in a floating state, the contrast of the image is low. More strictly, since there are a plurality of adherent cells in the biological sample, the higher the proportion of adherent cells in the adherent state in the adherent cells in the biological sample, the higher the contrast of the image. Thus, since there is a strong correlation between the state of the adherent cells and the contrast of the image, the determination unit 40b may determine the state of the adherent cells based on the contrast of the image.

  Moreover, the determination part 40b may specify the form of an adherent cell from the image data which the image data generation apparatus 20 produced | generated, for example, and may determine the state of an adherent cell based on the identified form. An example of the form of adherent cells is the shape of adherent cells. For example, a spherical cell in a floating state is deformed by adhesion in an adhesive state, and thus has a distorted shape (for example, an ellipse) different from the spherical shape. Thus, since there is a strong correlation between the state of the adherent cell and the shape of the adherent cell, the determination unit 40b identifies the shape by detecting the outline of the adherent cell by image processing, and based on the identified shape. Thus, the state of adherent cells may be determined.

  FIG. 9 is a flowchart showing an example of the cultured cell analysis process according to the present embodiment. FIG. 10 is another example of the input screen. Hereinafter, the cultured cell analysis process performed in the cultured cell analysis system will be specifically described with reference to FIGS. 9 and 10.

  When the cultured cell analysis process shown in FIG. 9 is started, the server 40 first acquires imaging conditions (step S100). Here, the server 40 displays, for example, the input screen 200 shown in FIG. 10 on the liquid crystal display 50. When the user inputs imaging conditions on the input screen 200 using the keyboard 60, the server 40 acquires the input imaging conditions and stores them in the imaging condition storage unit 40 f that is the memory 42 or the storage 43. In FIG. 10, the image data size is operated by operating the drop-down list 101, and the contrast of an image serving as a threshold for determining the adhesion state by operating the slider 201 is operated by the drop-down list 103. In this example, the scan interval is selected, and the number of scans is selected by operating the drop-down list 104.

  Next, the server 40 outputs an imaging instruction to the image data generation device 20 (step S110). Thereafter, the server 40 receives the image data from the image data generation device 20 (step S120), and pre-analyzes the image data (step S130). In this example, the pre-analysis process is a process for calculating the contrast of the image.

  In addition, if it is a case where the state of an adherent cell is determined based on the shape of an adherent cell, the prior analysis process may be a process for specifying the shape of an adherent cell. Further, the pre-analysis process may be a process of specifying a shape and further calculating a ratio of adherent cells having a predetermined shape (or other than the predetermined shape).

  When the preliminary analysis is completed, the server 40 determines the state of the adherent cell (step S140). Here, the server 40 determines the state of adherent cells based on the preliminary analysis result in step S130. In this example, the server 40 determines that the state of the adherent cell is in an adherent state when the contrast of the image calculated in Step S130 is equal to or greater than the contrast threshold acquired in Step S100, and the state of the adherent cell is less than the threshold. Judged to be floating.

  When the server 40 determines that the state of the adherent cell is not an adherent state (NO in step S150), the server 40 waits for a predetermined time (step S160), and repeats the processing from step S110 to step S150. On the other hand, when the server 40 determines that the state of the adherent cell is an adherent state (YES in step S150), the server 40 performs a time lapse control process (step S170), analyzes the image data acquired by the time lapse control process (step S180), A result is displayed (step S190) and a cultured cell analysis process is complete | finished. Note that the processing from step S170 to step S190 is the same as the processing from step S50 to step S70 in FIG.

  Even with the cultured cell analysis system according to the present embodiment, the same effect as that of the cultured cell analysis system 1 can be obtained. That is, it is possible to provide the user with an analysis result with higher accuracy than the conventional analysis system. Moreover, it is the same as that of the cultured cell analysis system 1 in that the analysis efficiency and the user's work efficiency are superior to the conventional analysis system.

  Furthermore, in the cultured cell analysis system according to this embodiment, the state of adherent cells is determined based on image data generated by imaging a biological sample. That is, the state of the actual adherent cell is confirmed from the image data, and the state of the adherent cell is determined based on the confirmation result. For this reason, according to the cultured cell analysis system which concerns on this embodiment, the state of an adherent cell can be determined with a higher precision than the cultured cell analysis system 1. FIG.

  Moreover, in the cultured cell analysis system 1, in order to ensure high determination accuracy, it is assumed that the start delay time is estimated and set to be somewhat longer. In contrast, in the cultured cell analysis system according to the present embodiment, since the time-lapse control process is started as soon as the state of the adherent cell is monitored and the adherent state is reached, the observation is started earlier than the cultured cell analysis system 1. Analysis results can be obtained.

[Third Embodiment]
The cultured cell analysis system according to the present embodiment includes the server 90 instead of the server 40, and the analysis target data specified by specifying the analysis target data from the image data obtained by the time lapse control process after the time lapse control process. Is different from the cultured cell analysis system according to the first and second embodiments.

  FIG. 11 is an example of a functional block diagram of the server 90. The server 90 includes an imaging condition acquisition unit 90a, a determination unit 90b, an imaging control unit 90c, an analysis unit 90d, a display control unit 90e, an imaging condition storage unit 90f, a time-lapse image storage unit 90g, and an analysis result storage unit 90h. Yes.

  The imaging condition acquisition unit 90a, the display control unit 90e, the imaging condition storage unit 90f, and the analysis result storage unit 90h are the imaging condition acquisition unit 40a, the display control unit 40e, the imaging condition storage unit 40f, and the analysis result storage unit 40h in FIG. It is the same.

  The imaging control unit 90c controls the image data generation device 20 so as to image a biological sample at a time interval (scan interval) preset in the imaging condition storage unit 90f. This is the same as the imaging control unit 40c. However, the imaging control unit 90c is different from the imaging control unit 40c in that the time lapse control process is started immediately after the imaging condition acquisition unit 90a acquires the imaging condition.

  The time lapse image storage unit 90g stores the image data acquired by the time lapse control process. This is the same as the time-lapse image storage unit 40g. However, the time-lapse image storage unit 90g is different from the time-lapse image storage unit 40g in that it stores image data generated by the image data generation device 20 regardless of the state of adherent cells.

  The determination unit 90b reads the image data stored in the time-lapse image storage unit 90g, and determines the state of adherent cells based on the read image data. Note that the determination method is the same as the determination method in the determination unit 40b according to the second embodiment. That is, the state of adherent cells may be determined based on the contrast of the image, or the state of adherent cells may be determined based on the shape of the adherent cells.

  The analysis unit 90d specifies analysis target data from the image data generated by the image data generation device 20 based on the determination result by the determination unit 90b. Then, the specified analysis target data is analyzed and stored in the analysis result storage unit 90h.

  FIG. 12 is a flowchart showing an example of the cultured cell analysis process according to the present embodiment. Hereinafter, the cultured cell analysis process performed in the cultured cell analysis system will be specifically described with reference to FIG.

  When the cultured cell analysis process shown in FIG. 12 is started, the server 90 first acquires imaging conditions (step S200), and then performs a time lapse control process (step S210). Note that the processes in steps S200 and S210 are the same as steps S100 and S170 in FIG.

  Next, the server 90 reads out the image data acquired by the time lapse control process from the time lapse image storage unit 90g, performs pre-analysis (step S220), and determines the state of the adherent cells (step S230). Note that the processes in steps S220 and S230 are the same as steps S130 and S140 in FIG.

  Thereafter, the server 90 specifies analysis target data from the image data stored in the time-lapse image storage unit 90g based on the determination result in step S230 (step S240).

  Finally, the server 90 analyzes the specified analysis target data (step S250), displays the analysis result (step S260), and ends the cultured cell analysis process. Note that the processing in step S250 and step S260 is the same as the processing in step S180 and step S190 in FIG.

  The cultured cell analysis system according to the present embodiment also provides the user with an analysis result with higher accuracy than the conventional analysis system, similarly to the cultured cell analysis system according to the first embodiment and the second embodiment. be able to.

  In the cultured cell analysis system according to the present embodiment, the analysis process can be performed at an arbitrary timing without being limited by the timing of performing the time-lapse observation. For this reason, for example, image data acquired by another analysis system can be analyzed.

DESCRIPTION OF SYMBOLS 10 Incubator 20 Image data generation apparatus 21 Image | photographing area | region 22 Image sensor 23 LED light source 24 for illumination Temperature sensor 30 Microplate 31 Well 40, 90 Server 40a, 90a Imaging condition acquisition part 40b, 90b Determination part 40c, 90c Imaging control part 40d, 90d Analysis unit 40e, 90e Display control unit 40f, 90f Imaging condition storage unit 40g, 90g Time-lapse image storage unit 40h, 90h Analysis result storage unit 41 Processor 42 Memory 43 Storage 44 Interface device 45 Portable storage medium drive unit 46 Portable storage Medium 47 Bus 50 Liquid crystal display 60 Keyboard 70 Notebook computer 80 Tablet computer 100, 200 Input screen 101, 102, 103 Drop-down list 201 Slider G1, G2

Claims (10)

An image data generation device for imaging a biological sample containing adherent cells and generating image data;
A determination unit for determining whether the state of the adherent cell is an adhesive state or a floating state; and
An analysis unit for analyzing data to be analyzed, which is image data generated by the image data generation device and is image data of the biological sample in the adhesion state specified based on a determination result by the determination unit; A cultured cell analysis system comprising: In the cultured cell analysis system according to claim 1,
The said determination part determines the state of the said adherent cell based on the elapsed time from reference | standard time, The cultured cell analysis system characterized by the above-mentioned. In the cultured cell analysis system according to claim 1,
The said determination part determines the state of the said adherent cell based on the image data which the said image data generation apparatus produced | generated, The cultured cell analysis system characterized by the above-mentioned. In the cultured cell analysis system according to claim 3,
The determination unit
Calculate the contrast of the image of the biological sample from the image data generated by the image data generation device,
A cultured cell analysis system, wherein the state of the adherent cell is determined based on the calculated contrast. In the cultured cell analysis system according to claim 3,
The determination unit
Identify the form of the adherent cells from the image data generated by the image data generation device,
A cultured cell analysis system, wherein the state of the adherent cell is determined based on the identified form. The cultured cell analysis system according to any one of claims 1 to 5, further comprising:
An imaging control unit for controlling the image data generation device;
When the determination unit determines that the state of the adherent cell is the adhesion state, the imaging control unit controls the image data generation device to image the biological sample at a preset time interval,
The analysis unit specifies the image data generated by the image data generation device as the analysis target data after the determination unit determines that the state of the adherent cell is the adherent state. Analysis system. The cultured cell analysis system according to any one of claims 1 to 5, further comprising:
An imaging control unit that controls the image data generation device so as to image the biological sample at a preset time interval;
The cultured cell analysis system, wherein the analysis unit specifies the analysis target data from image data generated by the image data generation device based on a determination result by the determination unit. The cultured cell analysis system according to any one of claims 1 to 7, further comprising:
A cultured cell analysis system comprising: a display control unit that displays a result of analysis by the analysis unit on a display device. Imaging biological samples containing adherent cells to generate image data,
Determine whether the state of the adherent cell is an adherent state or a floating state,
Cultured cell analysis characterized by analyzing analysis target data, which is generated image data and is image data of the biological sample in the adherent state specified based on a determination result about the state of the adherent cell Method. On the computer,
Obtain image data of biological samples containing adherent cells,
Determine whether the state of the adherent cell is an adherent state or a floating state,
A process of analyzing analysis target data, which is acquired image data, which is image data of the biological sample in the adherent state specified based on a determination result on the state of the adherent cells, Program to do.

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