JP2018173429A5 - - Google Patents

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JP2018173429A5
JP2018173429A5 JP2018153931A JP2018153931A JP2018173429A5 JP 2018173429 A5 JP2018173429 A5 JP 2018173429A5 JP 2018153931 A JP2018153931 A JP 2018153931A JP 2018153931 A JP2018153931 A JP 2018153931A JP 2018173429 A5 JP2018173429 A5 JP 2018173429A5
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measuring
substance
latex particles
measured
latex
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JP2018153931A
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JP6739481B2 (en
JP2018173429A (en
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Claims (8)

血液試料中の測定対象物質をラテックス免疫凝集法により測定する方法であって、
以下の工程を含む、前記測定方法。
(1)液相中で、当該試料とイミダゾール又はその誘導体あるいはこれらの塩とを接触させる工程
(2)(1)工程の後に、当該測定対象物質に対する特異的親和性物質を担持するラテックス粒子を液相中に添加する工程
(3)(2)の工程の後に、当該測定対象物質と当該ラテックス粒子の凝集反応を光学的に測定する工程
A method of measuring a substance to be measured in a blood sample by latex immunoagglutination,
The said measuring method including the following processes.
(1) Step of contacting the sample with imidazole or its derivative or a salt thereof in the liquid phase (2) After the step (1), latex particles carrying a specific affinity substance for the substance to be measured Step of adding to liquid phase (3) Step of optically measuring agglomeration reaction between the substance to be measured and the latex particles after the steps of (2)
(3)の光学的に測定する工程が、散乱光強度、吸光度、又は透過光強度を光学機器で測定する工程である請求項1に記載の測定方法。The measuring method according to claim 1, wherein the step of optically measuring (3) is a step of measuring scattered light intensity, absorbance, or transmitted light intensity with an optical device. (3)の光学的に測定する工程が、試料中に含まれている測定対象物の濃度を算出する工程を含む請求項1又は2に記載の測定方法。The measuring method according to claim 1 or 2, wherein the optically measuring step (3) includes a step of calculating the concentration of the measurement object contained in the sample. (3)の光学的に測定する工程が、測定対象物と特異的親和性物質を担持するラテックス粒子のサンドイッチ型の免疫複合体同士の凝集度合いを測定するものである請求項1〜3のいずれかに記載の測定方法。The optically measuring step of (3) measures the degree of aggregation between sandwich-type immune complexes of latex particles carrying a measurement target and a specific affinity substance. The measuring method of crab. 測定対象物質に対する特異的親和性物質を担持するラテックス粒子を2種類含む請求項1〜4のいずれかに記載の測定方法。The measurement method according to any one of claims 1 to 4, comprising two types of latex particles carrying a substance having a specific affinity for the substance to be measured. 血液試料が血清又は血漿である、請求項1〜4のいずれかに記載の方法。 The method according to any one of claims 1 to 4 , wherein the blood sample is serum or plasma. (3)のラテックス粒子の凝集反応を測定する工程のイミダゾール又はその誘導体あるいはこれらの塩の濃度が37.5mM以上375mM以下である、請求項1〜6のいずれかに記載の方法。 The method according to any one of claims 1 to 6 , wherein the concentration of imidazole or a derivative thereof or a salt thereof in the step (3) of measuring the aggregation reaction of latex particles is 37.5 mM or more and 375 mM or less. ラテックス免疫凝集法が、ホモジーニアス法にもとづく方法である請求項のいずれかに記載の方法。
The method according to any one of claims 1 to 7 , wherein the latex immunoaggregation method is a method based on the homogeneous method.
JP2018153931A 2018-08-20 2018-08-20 Method of reducing measurement error in latex immunoaggregation method Active JP6739481B2 (en)

Priority Applications (1)

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JP2018153931A JP6739481B2 (en) 2018-08-20 2018-08-20 Method of reducing measurement error in latex immunoaggregation method

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JP2016071275A Division JP6409016B2 (en) 2016-03-31 2016-03-31 Measurement error reduction method in latex immunoaggregation

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JP2018173429A JP2018173429A (en) 2018-11-08
JP2018173429A5 true JP2018173429A5 (en) 2019-08-29
JP6739481B2 JP6739481B2 (en) 2020-08-12

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Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10239317A (en) * 1996-12-28 1998-09-11 Shinotesuto:Kk Method and reagent for restrainedly measuring zone phenomenon suppression and measuring reagent
US20090117668A1 (en) * 2006-03-31 2009-05-07 Nissui Pharmaceutical Co., Ltd. Immune Agglutination Reagent Kit and Method of Measuring Antigen
JP2009186202A (en) * 2008-02-04 2009-08-20 Panasonic Corp Sensor apparatus and target substance detecting method
JP5807300B2 (en) * 2008-11-18 2015-11-10 株式会社シノテスト Method and reagent for measuring C-reactive protein in sample
WO2011158798A1 (en) * 2010-06-14 2011-12-22 国立大学法人山口大学 Method for observation and early prediction of clinical course of therapeutic effect on psoriasis, and kit for use in the method
JP5541747B2 (en) * 2012-03-26 2014-07-09 株式会社Lsiメディエンス Immunoassay and kit for small molecules
JP2016031250A (en) * 2014-07-25 2016-03-07 株式会社堀場製作所 Target protein measurement reagent, and measurement method using the same

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