JP2018078863A - Milk feeling improver - Google Patents
Milk feeling improver Download PDFInfo
- Publication number
- JP2018078863A JP2018078863A JP2016225194A JP2016225194A JP2018078863A JP 2018078863 A JP2018078863 A JP 2018078863A JP 2016225194 A JP2016225194 A JP 2016225194A JP 2016225194 A JP2016225194 A JP 2016225194A JP 2018078863 A JP2018078863 A JP 2018078863A
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- JP
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- Prior art keywords
- milk
- yeast extract
- milk feeling
- feeling
- food
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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Landscapes
- Dairy Products (AREA)
- Confectionery (AREA)
- Tea And Coffee (AREA)
- Seasonings (AREA)
- Non-Alcoholic Beverages (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
Description
本発明は、甘味料と酵母エキスとを有効成分とするミルク感向上剤およびこれを用いるミルク感が向上した飲食品の製造方法に関する。 The present invention relates to a milk feeling improving agent comprising a sweetener and a yeast extract as active ingredients, and a method for producing a food or drink with improved milk feeling using the same.
カフェオレやミルクティー、アイスクリーム、プリン、クリームスープ、グラタンなど、生乳や乳製品を配合した飲食品は、広く市場に展開されている。これらの飲食品において、乳の風味(ミルク感)は美味しさを形成する重要な要素であり、また、昨今では、ミルク感がより強いものが消費者に好まれる傾向がある。このことから、ミルク感を強調し、「ミルク感たっぷり」や「濃厚ミルク感」というように、そのことを宣伝文句とした商品の開発も行われている。 Foods and drinks that contain raw milk and dairy products such as cafe au lait, milk tea, ice cream, pudding, cream soup, and gratin are widely available in the market. In these foods and beverages, the flavor of milk (milk feeling) is an important factor for forming the deliciousness, and recently, consumers tend to prefer products having a stronger milk feeling. From this, emphasis is placed on the milk feeling, and products such as “a lot of milk feeling” and “a rich milk feeling” are being developed as advertising.
その一方で、脂肪分やカロリーを控えた商品が消費者に好まれる傾向もある。しかしながら、一般に、生乳や乳製品といったミルク感を有する飲食品材料には脂肪分が多く含まれ、カロリーも高く、その配合量を増加させると、製品の脂肪分やカロリーが増加してしまうという問題がある。 On the other hand, consumers tend to prefer products with reduced fat and calories. However, in general, food and drink materials having a milky feeling such as raw milk and dairy products contain a large amount of fat, high calories, and increasing the blending amount increases the fat and calories of the product. There is.
さらに、種々の飲食品材料の中で、生乳や乳製品の価格は比較的高く、また、近年、その価格は高騰傾向にある。このため、その配合量を増加させると、製品の製造コストが高くなってしまうという問題もある。 Furthermore, among various food and drink products, the price of raw milk and dairy products is relatively high, and in recent years, the price has been on a rising trend. For this reason, when the compounding quantity is increased, there also exists a problem that the manufacturing cost of a product will become high.
以上のことから、脂肪分やカロリーの増加あるいは製造コストの上昇を抑えつつ、ミルク感が強い飲食品を製造するために、ミルク感を有する材料の配合量を増加させずに、飲食品のミルク感を向上する方法が求められていた。この点、特許文献1には、ガラクトオリゴ糖を有効成分とする乳風味増強剤が([請求項1])、特許文献2には、スクラロースを乳含有製品に配合する乳感の増強方法が([請求項64])、それぞれ記載されている。 From the above, in order to produce a food or drink with a strong milk feeling while suppressing an increase in fat or calories or an increase in production cost, the milk of the food or drink is increased without increasing the amount of ingredients having a milk feeling. There was a need for a way to improve the feeling. In this regard, Patent Literature 1 discloses a milk flavor enhancer containing galactooligosaccharide as an active ingredient ([Claim 1]), and Patent Literature 2 discloses a method for enhancing milk feeling by blending sucralose into a milk-containing product ( [Claim 64]), respectively.
しかしながら、上記文献に記載された発明を鑑みても、いまだ飲食品のミルク感を向上する方法は十分に提供されておらず、そのような方法が求められていた。本発明は、このような課題を解決するためになされたものであって、飲食品のミルク感を効果的に向上することができる、ミルク感向上剤およびこれを用いるミルク感が向上した飲食品の製造方法を提供することを目的とする。 However, even in view of the invention described in the above literature, a method for improving the milky feeling of food and drink has not been sufficiently provided, and such a method has been demanded. The present invention has been made in order to solve such problems, and can improve the milk feeling of food and drink effectively, and the food and drink having improved milk feeling using the same. It aims at providing the manufacturing method of.
本発明者らは、ミルク感を有する材料を配合する飲食品の製造過程で、甘味料と酵母エキスとを併せて添加することにより、当該飲食品のミルク感および味の厚み(厚味)が顕著に強くなることを見出し、係る知見に基づいて、下記の各発明を完成した。 The inventors of the present invention have added a sweetener and a yeast extract in the course of manufacturing a food or drink that contains a milky feeling material, so that the milk feeling and taste thickness (thickness) of the food or drink can be increased. Based on this finding, the inventors have completed the following inventions.
(1)本発明に係るミルク感向上剤は、甘味料と酵母エキスとを有効成分とする。 (1) The milkiness improver according to the present invention comprises a sweetener and a yeast extract as active ingredients.
(2)本発明に係るミルク感向上剤において、甘味料は、砂糖、果糖、異性化糖、還元水飴、還元麦芽糖水飴、糖アルコールおよび高甘味度甘味料からなる群から選択される1または2以上であることが好ましい。 (2) In the milk feeling improving agent according to the present invention, the sweetener is 1 or 2 selected from the group consisting of sugar, fructose, isomerized sugar, reduced starch syrup, reduced maltose starch syrup, sugar alcohol and high-intensity sweetener. The above is preferable.
(3)本発明に係るミルク感向上剤において、酵母エキスは、乾燥重量当たり42重量%以上のタンパク質を含む酵母エキスであることが好ましい。 (3) In the milk feeling improving agent according to the present invention, the yeast extract is preferably a yeast extract containing 42% by weight or more of protein per dry weight.
(4)本発明に係るミルク感向上剤は、甘味料6重量部に対して、酵母エキスが0.001重量部超1重量部未満の割合で含有するものであることが好ましい。 (4) The milk feeling improving agent according to the present invention is preferably such that the yeast extract is contained in a proportion of more than 0.001 part by weight and less than 1 part by weight with respect to 6 parts by weight of the sweetener.
(5)本発明に係るミルク感向上剤は、厚味向上剤として用いることもできる。 (5) The milk feeling improving agent according to the present invention can also be used as a thickness improving agent.
(6)本発明に係るミルク感が向上した飲食品の製造方法は、本発明のミルク感向上剤を飲食品材料に添加する工程を有する。 (6) The manufacturing method of the food / beverage products which improved the milk feeling which concerns on this invention has the process of adding the milk feeling improvement agent of this invention to food / beverage products materials.
(7)本発明に係るミルク感が向上した飲食品の製造方法においては、酵母エキスが、飲食品材料と甘味料との合計100重量部に対して0.001重量部超1重量部未満であることが好ましい。 (7) In the method for producing a food or drink with improved milk feeling according to the present invention, the yeast extract is more than 0.001 part by weight and less than 1 part by weight with respect to 100 parts by weight of the total of the food and drink material and the sweetener. Preferably there is.
本発明に係るミルク感向上剤によれば、ミルク感を有する材料の配合量を増加させずとも、飲食品のミルク感や厚味を向上することができる。また、本発明に係る製造方法によれば、ミルク感を有する材料の配合量を増加させずとも、ミルク感や厚味が向上した飲食品を製造することができる。よって、本発明によれば、脂肪分やカロリーの増加あるいは製造コストの上昇を抑えつつ、豊かなミルク感や厚味を有する飲食品を製造することができる。 According to the milk feeling improving agent according to the present invention, the milk feeling and thickness of food and drink can be improved without increasing the blending amount of the material having a milk feeling. Moreover, according to the manufacturing method which concerns on this invention, the food / beverage products with improved milk feeling and thickness can be manufactured, without increasing the compounding quantity of the material which has a milk feeling. Therefore, according to this invention, the food / beverage products which have rich milk feeling and thickness can be manufactured, suppressing the increase in fat content or calories, or the raise of manufacturing cost.
以下、本発明に係るミルク感向上剤およびミルク感が向上した飲食品の製造方法について詳細に説明する。本発明において、「ミルク感」とは、乳の風味をいう。すなわち、ミルク感は、乳風味、ミルク風味、乳感などと同義に用いられる。 Hereinafter, the milk feeling improving agent according to the present invention and the method for producing a food or drink with improved milk feeling will be described in detail. In the present invention, “milk feeling” refers to the flavor of milk. That is, the milk feeling is used synonymously with milk flavor, milk flavor, milk feeling, and the like.
また、本発明において「厚味」とは、味の厚みをいい、ボディ感、コク、こってりした味などと同義に用いられる。本発明に係るミルク感向上剤は、ミルク感を向上するとともに厚味をも向上することから、厚味向上剤として、飲食品の厚味を向上する用途に用いることもできる。 In the present invention, the term “thickness” refers to the thickness of the taste, and is used synonymously with body feeling, richness, rich taste, and the like. Since the milk feeling improving agent which concerns on this invention improves a milk feeling and also improves thickness, it can also be used for the use which improves the thickness of food-drinks as a thickness improving agent.
飲食品のミルク感や厚味が向上したか否かは、官能試験により確認することができる。具体的には、まず、本発明のミルク感向上剤を配合した飲食品と、配合しない飲食品とを準備する。これをパネルに飲食させて、ミルク感や厚味の強弱を確認すればよい。前者の方がミルク感や厚味が強ければ、本発明のミルク感向上剤によりミルク感や厚味が向上したと判断することができる。 It can be confirmed by a sensory test whether the milk feeling and the thickness of the food or drink are improved. Specifically, first, a food / beverage product in which the milkiness improver of the present invention is blended and a food / beverage product not blended are prepared. This can be eaten and eaten on the panel to confirm the milk feel and the strength of the thickness. If the former is stronger in milk feeling and thickness, it can be determined that the milk feeling and thickness are improved by the milk feeling improving agent of the present invention.
本発明が対象とする飲食品は、ミルク感を有する材料を配合した飲食品である。ミルク感を有する材料としては、例えば、ヤギ、ウシ、馬、羊などの動物の乳や、これを加工してなる乳製品(牛乳、加工乳、ヨーグルト、クリーム、チーズ、バター、ホエイ、粉乳、練乳、バターミルク、発酵乳、乳脂、カゼインなど)の他、豆乳、植物性脂肪を主原料とするクリーム、液体や固体のコーヒークリーミング、マーガリンなどを挙げることができる。 The food / beverage products which this invention makes object are the food / beverage products which mix | blended the material which has a milk feeling. Examples of materials having a milk feeling include milk from animals such as goats, cows, horses, and sheep, and dairy products obtained by processing the milk (milk, processed milk, yogurt, cream, cheese, butter, whey, powdered milk, (Condensed milk, buttermilk, fermented milk, milk fat, casein, etc.), soy milk, creams mainly composed of vegetable fats, liquid or solid coffee creaming, margarine, and the like.
本発明に係るミルク感向上剤は、甘味料と酵母エキスとを有効成分とする。
ここで、「甘味料」は、飲食品に甘味を付与するために用いられる調味料をいう。本発明において、甘味料は、市販されているものを用いることができる。
The milk feeling improving agent according to the present invention contains sweeteners and yeast extract as active ingredients.
Here, the “sweetener” refers to a seasoning used for imparting sweetness to foods and drinks. In the present invention, commercially available sweeteners can be used.
本発明に係る甘味料として、具体的には、例えば、砂糖(ショ糖)、転化糖(ブドウ糖および果糖)、果糖、異性化糖、還元水飴、還元麦芽糖水飴、糖アルコール、高甘味度甘味料、蜂蜜、メープルシロップ、糖蜜、水飴、麦芽糖水飴、トレハロース、パラチノースなどを挙げることができる。
このうち、異性化糖として、より具体的には、例えば、ブドウ糖果糖液糖、果糖ブドウ糖液糖、高果糖液糖などを挙げることができる。
また、糖アルコールとして、より具体的には、例えば、エリスリトール、マルチトール、キシリトール、ソルビトール、マンニトール、ラクチトールなどを挙げることができる。
また、還元水飴として、より具体的には、例えば、高糖化還元水飴(単糖アルコール35〜55%、二糖アルコール25〜60%、三糖アルコール1〜25%、四糖アルコール0〜15%、五糖以上の糖アルコール0〜10%)、中糖化還元水飴(単糖アルコール1〜15%、二糖アルコール40〜60%、三糖アルコール10〜40%、四糖アルコール1〜10%、五糖以上の糖アルコール1〜40%)、低糖化還元水飴(単糖アルコール1〜15%、二糖アルコール5〜25%、三糖アルコール5〜25%、四糖アルコール1〜15%、五糖以上の糖アルコール45〜85%)などを挙げることができる。
また、高甘味度甘味料として、より具体的には、例えば、甘草抽出物やステビア抽出物、ラカンカ抽出物などの天然甘味料、アスパルテームやアセスルファムカリウム、スクラロース、サッカリン、ネオテームなどの合成甘味料を挙げることができる。
Specific examples of the sweetener according to the present invention include sugar (sucrose), invert sugar (dextrose and fructose), fructose, isomerized sugar, reduced starch syrup, reduced maltose starch syrup, sugar alcohol, and high-intensity sweetener. Honey, maple syrup, molasses, starch syrup, maltose syrup, trehalose, palatinose and the like.
Among these, specific examples of the isomerized sugar include glucose fructose liquid sugar, fructose glucose liquid sugar, and high fructose liquid sugar.
More specific examples of sugar alcohols include erythritol, maltitol, xylitol, sorbitol, mannitol, and lactitol.
More specifically, as reduced starch syrup, for example, highly saccharified reduced starch syrup (monosaccharide alcohol 35-55%, disaccharide alcohol 25-60%, trisaccharide alcohol 1-25%, tetrasaccharide alcohol 0-15% , Sugar alcohols of 5 or more sugars 0-10%), medium saccharified reduced starch syrup (monosaccharide alcohol 1-15%, disaccharide alcohol 40-60%, trisaccharide alcohol 10-40%, tetrasaccharide alcohol 1-10%, 5 to 5% sugar alcohol 1-40%), low glycated reduced starch syrup (1-15% monosaccharide alcohol, 5-25% disaccharide alcohol, 5-25% trisaccharide alcohol, 1-15% tetrasaccharide alcohol, 5 Sugar sugars of 45 to 85%).
More specifically, as the high-intensity sweetener, for example, natural sweeteners such as licorice extract, stevia extract, and rakanka extract, and synthetic sweeteners such as aspartame, acesulfame potassium, sucralose, saccharin, and neotame are used. Can be mentioned.
本発明においては、後述する実施例1に示すように、あらゆる甘味料が、酵母エキスと併用することによりミルク感向上作用において相乗効果を奏するが、好ましい甘味料は、砂糖、果糖、異性化糖、還元水飴、還元麦芽糖水飴、糖アルコール、高甘味度甘味料からなる群から選択される1または2以上である。 In the present invention, as shown in Example 1 described later, all sweeteners have a synergistic effect in improving milk feeling when used in combination with yeast extract. Preferred sweeteners include sugar, fructose, and isomerized sugar. 1 or 2 or more selected from the group consisting of reduced starch syrup, reduced maltose starch syrup, sugar alcohol, and high intensity sweetener.
「酵母エキス」とは、酵母の抽出物をいう。本発明に係る酵母エキスは、市販のものを用いてもよく、酵母から製造して得てもよい。好ましい酵母エキスとしては、乾燥重量当たり42重量%以上のタンパク質を含むものや、乾燥重量当たり15重量%超25重量%以下の遊離グルタミン酸を含むものを挙げることができる。 “Yeast extract” refers to an extract of yeast. As the yeast extract according to the present invention, commercially available products may be used, or they may be produced from yeast. Preferred yeast extracts include those containing 42 wt% or more protein per dry weight and those containing more than 15 wt% and 25 wt% or less free glutamic acid per dry weight.
酵母エキスは、自己消化法(酵母菌体内に本来あるタンパク質分解酵素等を利用して菌体を可溶化する方法)、酵素分解法(微生物や植物由来の酵素製剤を添加して菌体を可溶化する方法)、熱水抽出法(熱水中に一定時間浸漬して菌体を可溶化する方法)、酸あるいはアルカリ分解法(種々の酸あるいはアルカリを添加して菌体を可溶化する方法)、物理的破砕法(超音波処理や、高圧ホモジェナイズ法、グラスビーズ等の固形物と混合して混合・磨砕することにより菌体を破砕する方法)、凍結融解法(凍結および融解を1回以上行うことにより菌体を破砕する方法)等の定法に従って、酵母から製造することができる。
いずれの方法においても、一般には、酵母菌体が破壊され、残存菌体または菌体破砕物および酵母抽出物を含む酵母溶解物(粗エキスまたは酵母エキス原液とも称される)をそのまま濃縮、あるいはろ過および/または遠心分離によって残存菌体および菌体破砕物から分離して得られるろ液または上清(酵母抽出物)を濃縮し(濃縮工程)、場合によりさらに凍結乾燥等の処理を行って酵母エキス製品とする。
Yeast extract can be prepared by self-digestion method (method of solubilizing cells using proteolytic enzyme etc., which is inherent in yeast cells), enzyme decomposition method (addition of microorganism or plant-derived enzyme preparations) Solubilization method), hot water extraction method (method of solubilizing cells by soaking in hot water for a certain period of time), acid or alkali decomposition method (method of solubilizing cells by adding various acids or alkalis) ), Physical disruption method (sonication, high-pressure homogenization method, method of disrupting cells by mixing and grinding with solid materials such as glass beads), freeze-thaw method (freeze and thaw 1 It can be produced from yeast according to a conventional method such as a method for crushing bacterial cells by performing the treatment more than once.
In any method, generally, yeast cells are destroyed, and the yeast lysate (also referred to as a crude extract or a yeast extract stock solution) containing the remaining cells or the disrupted cells and yeast extract is concentrated as it is, or Concentrate the filtrate or supernatant (yeast extract) obtained by separating from the remaining cells and disrupted cells by filtration and / or centrifugation (concentration step), and optionally subject to further freeze-drying and other treatments Yeast extract product.
以下に、熱水抽出法による酵母エキスの製造例を示す。まず、酵母の培養液50mLを20℃、3000×gにて5分間遠心分離する。上清を除去して、ペレットを20mLの超純水に懸濁し、同条件にて遠心分離する操作を2回繰り返す。その後、ペレットを20mLの超純水に懸濁して酵母懸濁液を得る。この酵母懸濁液1.5mLをエッペンドルフチューブに移して、ブロックヒーターを用いて80℃にて30分間加熱することにより、抽出を行う。または、温浴を用いて100℃にて10分間加熱してもよい。その後、4℃、6000×gにて5分間遠心分離し、上清を回収することにより、酵母エキスを得ることができる。 Below, the manufacture example of the yeast extract by a hot-water extraction method is shown. First, 50 mL of yeast culture solution is centrifuged at 3000 × g for 5 minutes at 20 ° C. The operation of removing the supernatant, suspending the pellet in 20 mL of ultrapure water, and centrifuging under the same conditions is repeated twice. Thereafter, the pellet is suspended in 20 mL of ultrapure water to obtain a yeast suspension. Extraction is performed by transferring 1.5 mL of this yeast suspension to an Eppendorf tube and heating at 80 ° C. for 30 minutes using a block heater. Or you may heat for 10 minutes at 100 degreeC using a warm bath. Thereafter, the yeast extract can be obtained by centrifuging at 6000 × g for 5 minutes at 4 ° C. and collecting the supernatant.
本発明において、酵母エキスの形態は特に限定されず、液体であってもよく、固形状や粉末などの固体であってもよい。例えば、酵母エキスは、上述の製造例で製造したそのままの液体であってもよく、これを噴霧乾燥法やドラムドライ法、凍結乾燥法などの手法により乾燥させて粉末状にした乾燥粉末であってもよく、当該乾燥粉末を水やグリセリン、エタノールなど各種の溶媒に溶かしてなる液体であってもよい。 In the present invention, the form of the yeast extract is not particularly limited, and may be a liquid or a solid such as a solid or a powder. For example, the yeast extract may be a liquid as produced in the above production example, which is a dry powder obtained by drying the powder by a spray drying method, a drum drying method, a freeze drying method, or the like. Alternatively, a liquid obtained by dissolving the dry powder in various solvents such as water, glycerin, and ethanol may be used.
酵母エキス中のタンパク質量は、例えば、ケルダール法または燃焼法(改良デュマ法)により全窒素量を測定し、測定値に窒素・タンパク質換算係数6.25を乗じて求めることができる。 The amount of protein in the yeast extract can be determined, for example, by measuring the total nitrogen amount by the Kjeldahl method or the combustion method (modified Dumas method) and multiplying the measured value by the nitrogen / protein conversion factor of 6.25.
また、酵母エキス中の遊離グルタミン酸量は、例えば、日本電子社製アミノ酸自動分析装置JLC―500/V型や、(米国)ウォーターズ社製Acquity UPLC装置、王子計測機器社製BF―5のバイオセンサーなどを用いて、添付の使用書に従い測定することができる。このうち、BF―5のバイオセンサーは、グルタミン酸に特異的に反応する酵素電極を用いて溶液中のグルタミン酸を定量する装置であって、本酵素電極はタンパク質やペプチド中のグルタミン酸とは反応しない。よって、このような装置を用いることにより、遊離のグルタミン酸のみを選択的に定量することができる。BF―5のバイオセンサーを用いて測定する際は、酵母エキスの5倍希釈液に対して測定を行ない、検量線には1mMおよび5mMのグルタミン酸標準溶液を用いればよい。 The amount of free glutamic acid in the yeast extract is, for example, an amino acid automatic analyzer JLC-500 / V manufactured by JEOL Ltd., an Acquity UPLC device manufactured by Waters (USA), or a biosensor of BF-5 manufactured by Oji Scientific Instruments. Can be measured according to the attached instruction sheet. Among them, the BF-5 biosensor is an apparatus for quantifying glutamic acid in a solution using an enzyme electrode that specifically reacts with glutamic acid, and this enzyme electrode does not react with glutamic acid in proteins and peptides. Therefore, by using such an apparatus, only free glutamic acid can be selectively quantified. When measurement is performed using a BF-5 biosensor, measurement is performed on a 5-fold diluted solution of yeast extract, and 1 mM and 5 mM glutamic acid standard solutions may be used for the calibration curve.
酵母エキスの製造に用いる酵母は、炭素源、窒素源および無機塩等を含む液体培地を用いて定法に従い培養することができる。ここで、当該培養方法には、酵母の増殖が定常期に入った後に液体培地のpHを7.5以上11未満に調製し、当該pHの範囲内で更に培養する工程(pHシフト工程)を含むことが好ましい。特許第4757944号公報に記載のように、pHシフト工程を含む方法で培養することにより、乾燥酵母菌体重量当たり、4.0重量%〜10.0重量%の範囲で遊離グルタミン酸を含有する酵母を得ることができる。そして、当該酵母を用いることにより、乾燥重量当たり15重量%超25重量%以下の遊離グルタミン酸を含む酵母エキスを得ることができる。 Yeast used for the production of yeast extract can be cultured according to a conventional method using a liquid medium containing a carbon source, a nitrogen source, an inorganic salt and the like. Here, the culture method includes a step of adjusting the pH of the liquid medium to 7.5 or more and less than 11 after the yeast growth enters a stationary phase, and further culturing within the pH range (pH shift step). It is preferable to include. As described in Japanese Patent No. 4757944, yeast containing free glutamic acid in a range of 4.0 wt% to 10.0 wt% per dry yeast cell weight by culturing by a method including a pH shift step Can be obtained. And by using the said yeast, the yeast extract containing 15 to 25 weight% of free glutamic acid per dry weight can be obtained.
以下に、pHシフト工程を含む培養例を示す。
<前培養工程>
まず、700mLの糖蜜培地(糖蜜8質量%、尿素0.6質量%、硫酸アンモニウム0.16質量%、リン酸水素2アンモニウム0.08質量%)に酵母菌株300mLを植菌して前培養する。前培養の条件は、培養温度30℃、160rpmの振とう培養、培養時間は24時間とする。
<本培養工程>
続いて、前培養の培養液200mLを、2000mLの培地(塩化アンモニウム0.18質量%、リン酸水素2アンモニウム0.04質量%)に植菌し、流加培地として800mLの糖蜜(糖度36%)(最終8%)を用いて本培養する。本培養の条件は、培養温度30℃、3L/分および600rpmの通気攪拌培養とする。また、pH5.0で10質量%アンモニア水添加によるpHの下限制御を行う。pHの上限制御は無しとする。
<pHシフト工程>
次に、酵母が定常期に入った直後に、10質量%アンモニア水を添加することにより、培養液のpHをpH7〜11、好ましくは7.5以上11未満、より好ましくは8以上11未満のアルカリ性域に調整(pHシフト)し、当該pHの範囲内で更に酵母を培養する。本培養開始後48時間で培養を終了する。アンモニア水に代えて、アンモニアガスや尿素などのアルカリ物質を添加することによりpHシフトを行ってもよい。
Below, the example of culture | cultivation including a pH shift process is shown.
<Pre-culture process>
First, 300 mL of yeast strain is inoculated in 700 mL of molasses medium (molasses 8% by mass, urea 0.6% by mass, ammonium sulfate 0.16% by mass, diammonium hydrogen phosphate 0.08% by mass) and precultured. The pre-culture conditions are a culture temperature of 30 ° C., shaking culture at 160 rpm, and a culture time of 24 hours.
<Main culture process>
Subsequently, 200 mL of the preculture medium was inoculated into 2000 mL of a medium (ammonium chloride 0.18% by mass, diammonium hydrogen phosphate 0.04% by mass), and 800 mL of molasses (sugar content 36% as a fed-batch medium). ) (Final 8%). The conditions for the main culture are aeration and agitation culture at a culture temperature of 30 ° C., 3 L / min, and 600 rpm. Moreover, pH lower limit control is performed by adding 10% by mass ammonia water at pH 5.0. There is no upper limit control of pH.
<PH shift process>
Next, immediately after the yeast enters the stationary phase, 10% by mass of ammonia water is added to adjust the pH of the culture solution to pH 7 to 11, preferably 7.5 or more and less than 11, more preferably 8 or more and less than 11. The pH is adjusted to the alkaline range (pH shift), and the yeast is further cultured within the pH range. The culture is completed 48 hours after the start of the main culture. Instead of ammonia water, pH shift may be performed by adding an alkaline substance such as ammonia gas or urea.
酵母エキスが由来する酵母は、単細胞性の真菌類であればよい。本発明に係る酵母として、具体的には、例えば、サッカロマイセス(Saccharomyces)属菌、シゾサッカロマイセス(Shizosaccharomyces)属菌、ピキア(Pichia)属菌、キャンディダ(Candida)属菌、クリベロマイセス(Kluyveromyces)属菌、ウィリオプシス(Williopsis)属菌、デバリオマイセス(Debaryomyces)属菌、ガラクトマイセス(Galactomyces)属菌、トルラスポラ(Torulaspora)属菌、ロドトルラ(Rhodotorula)属菌、ヤロウィア(Yarrowia)属菌、ジゴサッカロマイセス(Zygosaccharomyces)属菌などを挙げることができる。これらのうち、可食性であることから、サッカロマイセス・セレビシエ(Saccharomyces cerevisiae)、キャンディダ・ユティリス(Candida utilis)、キャンディダ・トロピカリス(Candida tropicalis)、キャンディダ・リポリティカ(Candida lypolitica)、キャンディダ・サケ(Candida sake)などが好ましく、汎用されていることから、サッカロマイセス・セレビシエ(S. cerevisiae)やキャンディダ・ユティリス(C. utilis)がより好ましい。 The yeast from which the yeast extract is derived may be a unicellular fungus. Specific examples of the yeast according to the present invention include, for example, the genus Saccharomyces, the genus Shizosaccharomyces, the genus Pichia, the genus Candida, and the genus Kluyveromyces. Fungi, Williopsis, Debaryomyces, Galactomyces, Torulaspora, Rhodotorula, Yarrowia, Digosaccharomyces Zygosaccharomyces) and the like. Of these, because of their edible nature, Saccharomyces cerevisiae, Candida utilis, Candida tropicalis, Candida lypolitica, Candida lypolitica, Salmon (Candida sake) and the like are preferable and Saccharomyces cerevisiae and C. utilis are more preferable because they are widely used.
本発明に係る酵母として、より具体的には、例えば、特許第5717478号に記載の酵母を挙げることができる他、サッカロマイセス属菌として、S. cerevisiaeのAB9846株、AB1株(ビール酵母)、AB2株(協会ワイン4号)、AB5株(パン酵母)、AB6株(パン酵母)およびAB8株、ならびにSaccharomyces属AB4株(ワイン酵母)、Saccharomyces属AB7株(ウイスキー酵母)、Saccharomyces sakeAB9株(協会6号酵母)、Saccharomyces bayanus AB10株を挙げることができる。また、キャンディダ・ユティリス(C. utilis)として、C. utilisのJCM1624株、AB11株(IAM0626)、AB12株(IFO0639)、 AB13株(JCM2287が親株)、AB14株(JCM2287が親株)を挙げることができる。また、クリベロマイセス・ラクティス(K. lactis)として、K. lactisのAB15株(IFO1090)、AB16株を挙げることができる。 More specifically, examples of the yeast according to the present invention include yeasts described in Japanese Patent No. 5717478, and Saccharomyces sp. AB9846 strain, AB1 strain (beer yeast), AB2 Strain (Association Wine No. 4), AB5 strain (Baker's yeast), AB6 strain (Baker's yeast) and AB8 strain, and Saccharomyces genus AB4 strain (Wine yeast), Saccharomyces genus AB7 strain (Whisky yeast), Saccharomyces sake AB9 strain (Association 6) No. yeast) and Saccharomyces bayanus AB10 strain. Also, as C. utilis, C. utilis JCM1624 strain, AB11 strain (IAM0626), AB12 strain (IFO0639), AB13 strain (JCM2287 is the parent strain), AB14 strain (JCM2287 is the parent strain) Can do. Further, examples of K. lactis include K. lactis strain AB15 (IFO1090) and AB16 strain.
本発明に係るミルク感向上剤において、甘味料と酵母エキスとの含有割合は特に限定されないが、甘味料6重量部に対して酵母エキスが、好ましくは0.001重量部超1重量部以下、より好ましくは0.001重量部超0.5重量物未満、さらに好ましくは0.005重量部以上0.2重量部以下の含有割合を挙げることができる。後述する実施例2に示すように、当該含有割合においては、特に高いミルク感向上効果を得ることができる。 In the milk feeling improving agent according to the present invention, the content ratio of the sweetener and the yeast extract is not particularly limited, but the yeast extract is preferably more than 0.001 part by weight and 1 part by weight or less with respect to 6 parts by weight of the sweetener. More preferably, the content ratio is more than 0.001 part by weight and less than 0.5 part by weight, and more preferably 0.005 part by weight or more and 0.2 part by weight or less. As shown in Example 2 described later, a particularly high milk feeling improvement effect can be obtained in the content ratio.
本発明のミルク感向上剤は、後述する実施例2に示すように、飲食品の製造過程で飲食品材料に添加して用いることができる。本発明のミルク感向上剤は有効成分として甘味料を含有することから、飲食品の通常の製造工程において、甘味料を本発明のミルク感向上剤に置き換えて用いることができる。なお、甘味料と酵母エキスとは、同時に飲食品材料に添加してもよく、異なるタイミングで添加してもよい。 As shown in Example 2, which will be described later, the milk feeling improving agent of the present invention can be used by adding to the food or drink material in the process of producing the food or drink. Since the milkiness enhancer of the present invention contains a sweetener as an active ingredient, the sweetener can be used in place of the milkiness enhancer of the present invention in the normal production process of food and drink. In addition, a sweetener and a yeast extract may be added to food-drink material simultaneously, and may be added at a different timing.
本発明のミルク感向上剤の飲食品における添加量は特に限定されないが、例えば、飲食品材料と甘味料との合計100重量部に対して、酵母エキスの添加量が、好ましくは0.001重量部超1重量部以下、より好ましくは0.001重量部超0.5重量部未満、さらに好ましくは0.005重量部以上0.2重量部以下の添加量を上げることができる。後述する実施例2に示すように、当該添加量においては、特に高いミルク感向上効果を得ることができる。 Although the addition amount in the food / beverage products of the milk feeling improvement agent of this invention is not specifically limited, For example, the addition amount of a yeast extract with respect to a total of 100 weight part of food / beverage-materials and a sweetener, Preferably it is 0.001 weight. More than 1 part by weight and less than 1 part by weight, more preferably more than 0.001 part by weight and less than 0.5 part by weight, more preferably 0.005 part by weight and 0.2 part by weight can be increased. As shown in Example 2 to be described later, a particularly high milk feeling improving effect can be obtained with the addition amount.
最後に、本発明は、ミルク感が向上した飲食品の製造方法を提供する。本発明に係るミルク感が向上した飲食品の製造方法は、本発明のミルク感向上剤を飲食物材料に添加する工程を有する。なお、本製造方法において、上述した本発明のミルク感向上剤と同じまたは相当する発明特定事項については、再度の説明を省略する。 Finally, this invention provides the manufacturing method of the food / beverage products which the milk feeling improved. The manufacturing method of the food-drinks with which the milk feeling improved based on this invention has the process of adding the milk feeling improvement agent of this invention to food-drink material. In addition, in this manufacturing method, about the invention specific matter which is the same as or corresponds to the milk feeling improvement agent of this invention mentioned above, description for the second time is abbreviate | omitted.
以下、本発明に係るミルク感向上剤およびミルク感が向上した飲食品の製造方法について、各実施例に基づいて説明する。なお、本発明の技術的範囲は、これらの実施例によって示される特徴に限定されない。 Hereinafter, the milk feeling improving agent according to the present invention and the method for producing a food or drink with improved milk feeling will be described based on each example. Note that the technical scope of the present invention is not limited to the features shown by these examples.
以下の実施例では、15〜25重量%の遊離グルタミン酸を含む酵母エキスである「ハイパーミーストHG−PS(乾燥粉末;アサヒグループ食品社)」または「ハイパーミーストHG−PdD20(乾燥粉末;アサヒグループ食品社)」を、グリセリンに溶かしてなる液状化物(酵母エキス/グリセリン液状化物)を用いた。
また、甘味料は、別段の記載が無い限り、表1に示すものを用いた。表1中の「甘味度」は、各種の甘味料について、5質量%のショ糖水溶液と同等の甘味を呈する濃度(X質量%)を官能試験により決定し、次式1を用いて算出した。式1;甘味度=ショ糖水溶液の濃度(5質量%)/各種甘味料の水溶液濃度(X質量%)。
In the following examples, “Hypermeast HG-PS (Dry Powder; Asahi Group Food Company)” or “Hypermeast HG-PdD20 (Dry Powder; Asahi) which is a yeast extract containing 15 to 25% by weight of free glutamic acid. Group Food Company) "was used in a liquefied product (yeast extract / glycerin liquefied product) dissolved in glycerin.
The sweeteners shown in Table 1 were used unless otherwise specified. “Sweetness” in Table 1 was calculated using the following formula 1 by determining the concentration (X mass%) exhibiting sweetness equivalent to a 5 mass% sucrose aqueous solution for various sweeteners by a sensory test. . Formula 1: sweetness = concentration of aqueous sucrose solution (5 mass%) / aqueous concentration of various sweeteners (X mass%).
<実施例1>ミルク感向上効果の検討
(1)試料の調製
水に脱脂粉乳、表1に示す甘味料および酵母エキス/グリセリン液状化物を溶かして、No.1〜14の乳風味飲料を調製した。その配合を表2および表3に示す。なお、砂糖以外の甘味料の配合量は、それぞれの甘味度に基づいて、3.5gの砂糖の甘味度に相当する量とした。
<Example 1> Examination of milk feeling improving effect (1) Preparation of sample Nonfat dry milk, sweetener and yeast extract / glycerin liquefied product shown in Table 1 were dissolved in water. 1-14 milk flavored beverages were prepared. The formulations are shown in Tables 2 and 3. In addition, the compounding quantity of sweeteners other than sugar was made into the quantity corresponded to the sweetness degree of 3.5g sugar based on each sweetness degree.
(2)官能試験
本実施例1(1)の乳風味飲料について、10名の分析型パネルにより官能試験を行い、ミルク感の強さ(ミルク感強度)および味の厚み(厚味)の強さ(厚味強度)を点数で表した。
ミルク感強度および厚味強度の点数化は、No.1の試料(甘味料および酵母エキスのいずれも添加せず、脱脂粉乳のみを溶かした乳風味飲料)のミルク感強度および厚味強度をそれぞれ比較対照(3点)として、次の7段階のいずれに該当するかを各パネルが判断することにより行った。ミルク感強度または厚味強度:非常に強い(5点)、強い(4点)、同等(3点)、やや弱い(2点)、弱い(1点)。
その後、試料ごとに、全パネルによる採点結果の平均値を求めた。その結果を表2および表3の最下段に示す。
(2) Sensory test The milk flavored beverage of Example 1 (1) was subjected to a sensory test with an analysis panel of 10 persons, and the strength of milk feeling (milk feeling strength) and the strength of taste thickness (thickness) were strong. The thickness (thickness strength) was expressed in points.
The score of milk feeling strength and thickness strength is No. One sample (milk flavored beverage in which neither a sweetener nor a yeast extract was added and only skim milk powder was dissolved) was used as a comparative control (3 points) for each of the following seven steps. Each panel judged whether it corresponds to. Milk strength or thickness strength: very strong (5 points), strong (4 points), equivalent (3 points), slightly weak (2 points), weak (1 point).
Then, the average value of the scoring result by all the panels was calculated | required for every sample. The results are shown at the bottom of Table 2 and Table 3.
表2および表3の最下段に示すように、ミルク感強度は、No.1(脱脂粉乳のみ)の3点に対して、No.2(酵母エキスを添加)では3.5点、No.3(砂糖を添加)では3.2点、No.5(還元麦芽糖水飴を添加)では3.3点、No.7(中糖化還元水飴を添加)では3.2点、No.9(低糖化還元水飴を添加)では3.5点、No.11(エリスリトールを添加)では3.1点、No.13(ステビア抽出物を添加)では3.2点であった。
すなわち、酵母エキス、砂糖、還元麦芽糖水飴、中糖化還元水飴、低糖化還元水飴、エリスリトールまたはステビア抽出物を添加した乳風味飲料では、これらを添加しないものと比較して、ミルク感強度が大きかった。
この結果から、酵母エキスおよび甘味料は、いずれもミルク感を向上する作用を有することが明らかになった。
As shown in the bottom of Table 2 and Table 3, the milk feeling strength was No. No. 1 for three points (only skim milk powder). 2 (added yeast extract), 3.5 points, No. 3 (addition of sugar), 3.2 points, No. 5 (added reduced maltose starch syrup), 3.3 points, No. 5 7 (with medium saccharified reduced starch syrup), 3.2 points, No. 9 (with low saccharified reduced starch syrup), 3.5 points, No. 9 11 (added erythritol) 3.1 points, No. 13 (added stevia extract) was 3.2 points.
That is, milk flavored beverages with added yeast extract, sugar, reduced maltose starch syrup, medium saccharified reduced starch syrup, low saccharified reduced starch syrup, erythritol or stevia extract had higher milk feeling strength compared to those without addition of these. .
From these results, it was revealed that both the yeast extract and the sweetener have an action of improving the milk feeling.
さらに、ミルク感強度は、No.2(酵母エキスを添加)の3.5点、No.3(砂糖を添加)の3.2点に対して、No.4(酵母エキスおよび砂糖を添加)では4.5点と、顕著に大きくなった。
同様に、No.2の3.5点、No.5(還元麦芽糖水飴を添加)の3.3点に対して、No.6(酵母エキスおよび還元麦芽糖水飴を添加)では4.5点と、顕著に大きくなった。
また、No.2の3.5点、No.7(中糖化還元水飴を添加)の3.2点に対して、No.8(酵母エキスおよび中糖化還元糖水飴を添加)では5点と、顕著に大きくなった。
また、No.2の3.5点、No.9(低糖化還元水飴を添加)の3.5点に対して、No.10(酵母エキスおよび低糖化還元糖水飴を添加)では5点と、顕著に大きくなった。
また、No.2の3.5点、No.11(エリスリトールを添加)の3.1点に対して、No.12(酵母エキスおよびエリスリトールを添加)では4点と、顕著に大きくなった。
また、No.2の3.5点、No.13(ステビア抽出物を添加)の3.2点に対して、No.14(酵母エキスおよびステビア抽出物を添加)では5点と、顕著に大きくなった。
Furthermore, the milk feeling strength is No. 2 (added yeast extract) 3.5 points, No. 2 3 (added sugar) 3.2 points, No. In 4 (added yeast extract and sugar), it was significantly large at 4.5 points.
Similarly, no. 2, 3.5 points, no. No. 5 (added with reduced maltose starch syrup), No. 5 In 6 (yeast extract and reduced maltose starch syrup added), it was significantly increased to 4.5 points.
No. 2, 3.5 points, no. No. 7 (addition of medium saccharified reduced starch syrup), No. 7 In 8 (adding yeast extract and medium saccharified and reduced sugar syrup), it was significantly increased to 5 points.
No. 2, 3.5 points, no. No. 9 (added low saccharified reduced starch syrup), No. 9 10 (added yeast extract and low saccharified reducing sugar syrup) increased significantly to 5 points.
No. 2, 3.5 points, no. No. 11 (added erythritol), No. 12 (added yeast extract and erythritol) markedly increased to 4 points.
No. 2, 3.5 points, no. No. 13 (added stevia extract) No. In 14 (added yeast extract and stevia extract), it was significantly increased to 5 points.
すなわち、酵母エキスと、砂糖、還元麦芽糖水飴、中糖化還元水飴、低糖化還元水飴、エリスリトールまたはステビア抽出物とを添加した乳風味飲料では、いずれか一方のみを添加した乳風味飲料と比較して、ミルク感強度が顕著に大きかった。
この結果から、酵母エキスと甘味料とは、ミルク感向上作用において、相乗的に働くことが明らかになった。すなわち、酵母エキスと甘味料とを併せて添加することにより、ミルク感向上効果において相乗効果が得られることが明らかになった。また、当該相乗効果を奏する甘味料として、砂糖、還元麦芽糖水飴、還元水飴、糖アルコール、高甘味度甘味料などのあらゆる甘味料が使用可能であることが明らかになった。
In other words, in the milk flavored beverage added with yeast extract and sugar, reduced maltose starch syrup, medium saccharified reduced starch syrup, low saccharified reduced starch syrup, erythritol or stevia extract, compared with milk flavored beverages to which only one of them is added. The milk feeling intensity was remarkably large.
From this result, it was clarified that the yeast extract and the sweetener work synergistically in the milk feeling improving action. That is, it has been clarified that a synergistic effect can be obtained in the milk feeling improving effect by adding the yeast extract and the sweetener together. In addition, it has been clarified that any sweeteners such as sugar, reduced maltose starch syrup, reduced starch syrup, sugar alcohol, and a high-intensity sweetener can be used as sweeteners that exhibit the synergistic effect.
一方、厚味強度は、No.1(脱脂粉乳のみ)の3点に対して、No.2(酵母エキスを添加)、No.3(砂糖を添加)、No.5(還元麦芽糖水飴を添加)、No.7(中糖化還元水飴を添加)、No.9(低糖化還元水飴を添加)、No.11(エリスリトールを添加)およびNo.13(ステビア抽出物を添加)では、いずれも3.2点であった。
すなわち、酵母エキス、砂糖、還元麦芽糖水飴、中糖化還元水飴、低糖化還元水飴、エリスリトールまたはステビア抽出物を添加した乳風味飲料では、これらを添加しないものと比較して、厚味強度が大きかった。
この結果から、酵母エキスおよび甘味料は、いずれも厚味を向上する作用を有することが明らかになった。
On the other hand, the thick strength is no. No. 1 for three points (only skim milk powder). 2 (added yeast extract), No. 2 3 (added sugar), No. 3 5 (addition of reduced maltose starch syrup), No. 5 7 (addition of medium saccharified reduced starch syrup), No. 7 9 (added low saccharified reduced starch syrup), No. 9 11 (with addition of erythritol) and 13 (added stevia extract) was 3.2 points in all cases.
That is, in milk flavored beverages added with yeast extract, sugar, reduced maltose starch syrup, medium saccharified reduced starch syrup, low saccharified reduced starch syrup, erythritol or stevia extract, the thick strength was greater than those without these .
From these results, it was revealed that both the yeast extract and the sweetener have an effect of improving the thickness.
さらに、厚味強度は、No.4(酵母エキスおよび砂糖を添加)では4.1点、No.6(酵母エキスおよび還元麦芽糖水飴を添加)では4.2点、No.8(酵母エキスおよび中糖化還元糖水飴を添加)では4.2点、No.10(酵母エキスおよび低糖化還元糖水飴を添加)では4.2点、No.12(酵母エキスおよびエリスリトールを添加)では3.7点、No.14(酵母エキスおよびステビア抽出物を添加)では4.2点であり、酵母エキスまたは甘味料を単独で添加した場合と比較して、顕著に大きくなった。 Further, the thick strength is No. 4 (added yeast extract and sugar), 4.1 points, No. 6 (with yeast extract and reduced maltose starch syrup added), 4.2 points, no. No. 8 (added yeast extract and medium saccharified reducing sugar syrup) 4.2, No. 10 (added yeast extract and low saccharified reducing sugar syrup) 4.2, No. 12 (with yeast extract and erythritol added), 3.7 points, 14 (added yeast extract and stevia extract) was 4.2 points, which was significantly larger than the case where yeast extract or sweetener was added alone.
すなわち、酵母エキスと、砂糖、還元麦芽糖水飴、中糖化還元水飴、低糖化還元水飴、エリスリトールまたはステビア抽出物とを添加した乳風味飲料では、いずれか一方のみを添加した乳風味飲料と比較して、厚味強度が顕著に大きかった。
この結果から、酵母エキスと甘味料とは、厚味向上作用において、相乗的に働くことが明らかになった。すなわち、酵母エキスと甘味料とを併せて添加することにより、厚味向上効果において相乗効果が得られることが明らかになった。また、当該相乗効果を奏する甘味料として、砂糖、還元麦芽糖水飴、還元水飴、糖アルコール、高甘味度甘味料などのあらゆる甘味料が使用可能であることが明らかになった。
In other words, in the milk flavored beverage added with yeast extract and sugar, reduced maltose starch syrup, medium saccharified reduced starch syrup, low saccharified reduced starch syrup, erythritol or stevia extract, compared with milk flavored beverages to which only one of them is added. The thick strength was remarkably large.
From this result, it was clarified that the yeast extract and the sweetener work synergistically in improving the thickness. That is, it has been clarified that a synergistic effect can be obtained in the effect of improving the thickness by adding the yeast extract and the sweetener together. In addition, it has been clarified that any sweeteners such as sugar, reduced maltose starch syrup, reduced starch syrup, sugar alcohol, and a high-intensity sweetener can be used as sweeteners that exhibit the synergistic effect.
<実施例2>酵母エキスの添加量の検討
甘味料の添加量を6gに固定し、酵母エキスの添加量を変化させて、実施例1(1)に記載の方法により、No.15〜32の乳風味飲料を調製した。ただし、甘味料は還元麦芽糖水飴を用いた。その配合を表4、表5および表6に示す。
続いて、これらの乳風味飲料について、No.15の試料(還元麦芽糖水飴および酵母エキスのいずれも添加せず、脱脂粉乳のみを溶かした乳風味飲料)のミルク感強度および厚味強度をそれぞれ比較対照(3点)として、実施例1(2)に記載の方法により官能試験を行い、ミルク感強度および厚味強度を評価した。その結果を表4、表5および表6の最下段に示す。
<Example 2> Examination of addition amount of yeast extract The addition amount of sweetener was fixed at 6 g, and the addition amount of yeast extract was changed. 15-32 milk flavored beverages were prepared. However, reduced maltose starch syrup was used as a sweetener. The formulations are shown in Tables 4, 5 and 6.
Then, about these milk flavor drinks, it is No. Example 1 (2) using the milk feeling intensity and the thickness intensity of 15 samples (milk flavored beverages in which neither reduced maltose starch syrup nor yeast extract was added and only skim milk powder was dissolved) as comparative controls (3 points), respectively. ) Was subjected to a sensory test, and milk feeling strength and thickness strength were evaluated. The results are shown at the bottom of Table 4, Table 5 and Table 6.
表4、表5および表6の最下段に示すように、No.16(還元麦芽糖水飴を添加)の3.2点、No.17(酵母エキス0.001gを添加)の3.1点に対して、No.18(還元麦芽糖水飴および酵母エキス0.001gを添加)では3.2点であり、還元麦芽糖水飴および酵母エキスの併用によるミルク感向上効果は認められなかった。
一方、No.16の3.2点、No.19(酵母エキス0.005gを添加)の3.2点に対して、No.20(還元麦芽糖水飴および酵母エキス0.005gを添加)では3.5点であり、ミルク感強度は顕著に大きくなった。
同様に、No.16の3.2点、No.21(酵母エキス0.01gを添加)の3.5点に対して、No.22(還元麦芽糖水飴および酵母エキス0.01gを添加)では4.1点であり、ミルク感強度は顕著に大きくなった。
また同様に、No.16の3.2点、No.23(酵母エキス0.05gを添加)の3.6点に対して、No.24(還元麦芽糖水飴および酵母エキス0.05gを添加)では4.5点であり、ミルク感強度は顕著に大きくなった。
また同様に、No.16の3.2点、No.25(酵母エキス0.1gを添加)の3.7点に対して、No.26(還元麦芽糖水飴および酵母エキス0.1gを添加)では4.5点であり、ミルク感強度は顕著に大きくなった。
また同様に、No.16の3.2点、No.27(酵母エキス0.2gを添加)の3.8点に対して、No.28(還元麦芽糖水飴および酵母エキス0.2gを添加)では4.5点であり、ミルク感強度は顕著に大きくなった。
一方、No.16の3.2点、No.29(酵母エキス0.5gを添加)の3.6点に対して、No.30(還元麦芽糖水飴および酵母エキス0.5gを添加)では3.8点であり、ミルク感強度は大きくなった。
また、No.16の3.2点、No.31(酵母エキス1gを添加)の3.1点に対して、No.32(還元麦芽糖水飴および酵母エキス1gを添加)では3.3点であり、ミルク感強度は大きくなった。
As shown at the bottom of Table 4, Table 5 and Table 6, No. 16 (added reduced maltose starch syrup) 3.2 points, No. No. 17 (added 0.001 g of yeast extract), No. 18 (added 0.001 g of reduced maltose starch syrup and yeast extract) was 3.2 points, and the milk feeling improvement effect by the combined use of reduced maltose starch syrup and yeast extract was not recognized.
On the other hand, no. 16, 3.2 points, No. 16 19 (added 0.005 g yeast extract), No. 20 (added reduced maltose starch syrup and yeast extract 0.005 g) was 3.5 points, and the milk feeling strength was significantly increased.
Similarly, no. 16, 3.2 points, No. 16 No. 21 (added 0.01 g yeast extract), No. 22 (added reduced maltose starch syrup and 0.01 g of yeast extract) was 4.1 points, and the milk feeling strength was significantly increased.
Similarly, no. 16, 3.2 points, No. 16 23 (added 0.05 g of yeast extract), No. 23 24 (added reduced maltose starch syrup and 0.05 g of yeast extract) was 4.5 points, and the milk feeling strength was significantly increased.
Similarly, no. 16, 3.2 points, No. 16 No. 25 (added with 0.1 g yeast extract) No. 26 (added reduced maltose starch syrup and 0.1 g of yeast extract) was 4.5 points, and the milk feeling strength was significantly increased.
Similarly, no. 16, 3.2 points, No. 16 No. 27 (with 0.2 g yeast extract added), No. 28 (added reduced maltose starch syrup and 0.2 g of yeast extract) was 4.5 points, and the milk feeling strength was significantly increased.
On the other hand, no. 16, 3.2 points, No. 16 No. 29 (0.5 g of yeast extract added), No. 30 (added reduced maltose starch syrup and 0.5 g of yeast extract) was 3.8 points, and the milk feeling strength increased.
No. 16, 3.2 points, No. 16 No. 31 (added 1 g of yeast extract), No. 32 (added reduced maltose starch syrup and 1 g of yeast extract) was 3.3 points, and the milk feeling strength increased.
すなわち、還元麦芽糖水飴および酵母エキスの併用によるミルク感向上効果は、酵母エキス添加量が0.001g超1g以下で大きく、0.001g超0.5g未満で顕著に大きかった。
この結果から、甘味料と酵母エキスとを有効成分とするミルク感向上剤においては、甘味料6重量部に対して、酵母エキスが0.001重量部超1重量部以下であると、ミルク感向上作用が大きくなることが明らかになった。
また、当該ミルク感向上剤を飲食品材料に添加する場合は、飲食品材料と甘味料との合計100重量部に対して、酵母エキスの添加量が0.001重量部超1重量部以下であると、ミルク感向上作用が大きくなることが明らかになった。
That is, the effect of improving milk feeling by the combined use of reduced maltose starch syrup and yeast extract was large when the amount of yeast extract added was more than 0.001 g and less than 1 g, and markedly great when the amount was more than 0.001 g and less than 0.5 g.
From this result, in the milk feeling improving agent containing sweetener and yeast extract as active ingredients, the milk feeling is more than 0.001 part by weight and less than 1 part by weight with respect to 6 parts by weight of sweetener. It became clear that the improvement effect became large.
Moreover, when adding the said milk feeling improvement agent to food-drinks material, the addition amount of a yeast extract is more than 0.001 weight part and 1 weight part or less with respect to 100 weight part in total of food-drink material and sweetener. It became clear that there was a large milk feeling improving effect.
一方、厚味強度は、No.16(還元麦芽糖水飴を添加)の3.2点、No.17(酵母エキス0.001gを添加)の3.1点に対して、No.18(還元麦芽糖水飴および酵母エキス0.001gを添加)では3.2点であり、還元麦芽糖水飴および酵母エキスの併用による厚味向上効果は認められなかった。
しかし、No.16の3.2点、No.19(酵母エキス0.005gを添加)の3.1点に対して、No.20(還元麦芽糖水飴および酵母エキス0.005gを添加)では3.4点であり、厚味強度は顕著に大きくなった。
同様に、No.16の3.2点、No.21(酵母エキス0.01gを添加)の3.5点に対して、No.22(還元麦芽糖水飴および酵母エキス0.01gを添加)では3.8点であり、厚味強度は顕著に大きくなった。
その一方で、No.16の3.2点、No.23(酵母エキス0.05gを添加)の3.6点に対して、No.24(還元麦芽糖水飴および酵母エキス0.05gを添加)では3.5点であり、還元麦芽糖水飴および酵母エキスの併用による厚味向上効果は認められなかった。
しかし、No.16の3.2点、No.25(酵母エキス0.1gを添加)の3.6点に対して、No.26(還元麦芽糖水飴および酵母エキス0.1gを添加)では3.8点であり、厚味強度は大きくなった。
また、No.16の3.2点、No.27(酵母エキス0.2gを添加)の3.7点に対して、No.28(還元麦芽糖水飴および酵母エキス0.2gを添加)では4.2点であり、厚味強度は顕著に大きくなった。
また、No.16の3.2点、No.29(酵母エキス0.5gを添加)の3.4点に対して、No.30(還元麦芽糖水飴および酵母エキス0.5gを添加)では3.6点であり、厚味強度は大きくなった。
しかし、No.16の3.2点、No.31(酵母エキス1gを添加)の3.1点に対して、No.32(還元麦芽糖水飴および酵母エキス1gを添加)では3点であり、還元麦芽糖水飴および酵母エキスの併用による厚味向上効果は認められなかった。
On the other hand, the thick strength is no. 16 (added reduced maltose starch syrup) 3.2 points, No. No. 17 (added 0.001 g of yeast extract), No. 18 (added 0.001 g of reduced maltose starch syrup and yeast extract) was 3.2 points, and the effect of improving the thickness by the combined use of reduced maltose starch syrup and yeast extract was not observed.
However, no. 16, 3.2 points, No. 16 No. 19 (added 0.005 g of yeast extract), No. 20 (added reduced maltose starch syrup and 0.005 g of yeast extract) was 3.4 points, and the thickness strength was significantly increased.
Similarly, no. 16, 3.2 points, No. 16 No. 21 (added 0.01 g yeast extract), No. 22 (added reduced maltose starch syrup and 0.01 g of yeast extract) was 3.8 points, and the thickness strength was significantly increased.
On the other hand, no. 16, 3.2 points, No. 16 23 (added 0.05 g of yeast extract), No. 23 24 (added 0.05 g of reduced maltose starch syrup and yeast extract) was 3.5 points, and the effect of improving the thickness by the combined use of reduced maltose starch syrup and yeast extract was not observed.
However, no. 16, 3.2 points, No. 16 No. 25 (added with 0.1 g yeast extract), No. No. 26 (reduced maltose syrup and 0.1 g of yeast extract added) had 3.8 points and increased thickness strength.
No. 16, 3.2 points, No. 16 No. 27 (added 0.2 g of yeast extract) No. 28 (added reduced maltose starch syrup and 0.2 g of yeast extract) was 4.2 points, and the thickness strength was significantly increased.
No. 16, 3.2 points, No. 16 No. 29 (added 0.5 g yeast extract), No. 30 (added reduced maltose starch syrup and 0.5 g of yeast extract) scored 3.6 points, increasing the thickness strength.
However, no. 16, 3.2 points, No. 16 No. 31 (added 1 g of yeast extract), No. 32 (added 1 g of reduced maltose starch syrup and yeast extract) was 3 points, and the effect of improving the thickness by the combined use of reduced maltose starch syrup and yeast extract was not observed.
すなわち、還元麦芽糖水飴および酵母エキスの併用による厚味向上効果は、酵母エキス添加量が0.001g超0.05g未満および0.05g超1g未満で大きく、0.005g以上0.01g以下および0.1g超0.5g未満で顕著に大きかった。
この結果から、甘味料と酵母エキスとを有効成分とするミルク感向上剤においては、甘味料6重量部に対して、酵母エキスが0.001重量部超0.05重量部未満または0.05重量部超1重量部未満であると、厚味向上作用が大きくなることが明らかになった。
また、当該ミルク感向上剤を飲食品材料に添加する場合は、飲食品材料と甘味料との合計100重量部に対して、酵母エキスの添加量が0.001重量部超0.05重量部未満または0.05重量部超1重量部未満であると、厚味向上作用が大きくなることが明らかになった。
That is, the effect of improving the thickness by the combined use of reduced maltose starch syrup and yeast extract is large when the amount of yeast extract added is more than 0.001 g and less than 0.05 g and more than 0.05 g and less than 1 g, 0.005 g to 0.01 g and 0 More than 1 g and less than 0.5 g was remarkably large.
From this result, in the milk feeling improver comprising sweetener and yeast extract as active ingredients, yeast extract is more than 0.001 part by weight and less than 0.05 part by weight or 0.05 part by weight with respect to 6 parts by weight of sweetener. It has been clarified that when the amount is more than 1 part by weight and less than 1 part by weight, the effect of improving the thickness is increased.
Moreover, when adding the said milk feeling improvement agent to food-drinks material, the addition amount of a yeast extract is more than 0.001 weight part 0.05 weight part with respect to 100 weight part in total of food-drink material and sweetener. It has been clarified that when the amount is less than or more than 0.05 parts by weight and less than 1 part by weight, the effect of improving the thickness is increased.
<実施例3>アイスクリームの製造
比較例として、ミルク感向上剤を添加しないアイスクリームを定法に従って製造した。オーバーランは70%に設定した。また、実施例として、ミルク感向上剤(中糖化還元水飴および酵母エキス)を添加し、かつ、ミルク感を有する材料(脱脂粉乳)の配合量を低減したアイスクリームを同様に製造した。それらの配合を表7に示す。なお、実施例においては、比較例と同等の甘味にするため、水飴の添加量を低減させた。
続いて、これらのアイスクリームについて、実施例1(2)に記載の方法により官能試験を行い、ミルク感強度および厚味強度を評価した。ミルク感強度および厚味強度の点数化では、比較例のアイスクリームを比較対照(3点)とした。その結果を表7の最下段に示す。
<Example 3> Manufacture of ice cream As a comparative example, an ice cream to which no milk feeling improver was added was manufactured according to a conventional method. Overrun was set at 70%. Moreover, as an Example, the ice cream which added the milk feeling improvement agent (medium saccharified reduced starch syrup and yeast extract), and reduced the compounding quantity of the material (skim milk powder) which has a milk feeling was manufactured similarly. Their formulations are shown in Table 7. In addition, in the Example, in order to make sweetness equivalent to a comparative example, the addition amount of the starch syrup was reduced.
Subsequently, these ice creams were subjected to a sensory test by the method described in Example 1 (2) to evaluate the milk feeling strength and the thickness strength. In scoring milk feeling strength and thickness strength, the comparative ice cream was used as a comparative control (3 points). The results are shown at the bottom of Table 7.
表7の最下段に示すように、ミルク感強度および厚味強度は、比較例の3点に対して、実施例ではいずれも4点であった。すなわち、ミルク感を有する材料(脱脂粉乳)の配合量を低減したにもかかわらず、ミルク感強度および厚味強度のいずれも、比較例よりも実施例の方が顕著に大きかった。
この結果から、甘味料と酵母エキスとを有効成分とするミルク感向上剤を添加することにより、ミルク感を有する材料の配合量を低減しても、低減しない場合と同等以上にミルク感および厚味が向上したアイスクリームを製造できることが明らかになった。
As shown in the lowermost row of Table 7, the milk feeling strength and the thickness strength were all 4 in the example compared to 3 in the comparative example. That is, although the blending amount of the material having milk feeling (skimmed milk powder) was reduced, both the milk feeling strength and the thickness strength were significantly larger in the example than in the comparative example.
From these results, it was found that adding a milk feeling improver containing sweeteners and yeast extract as active ingredients reduced the milk feeling and thickness to the same level as when the amount of ingredients having a milk feeling was not reduced. It became clear that ice cream with improved taste could be produced.
<実施例4>ミルクコーヒーの製造
比較例として、ミルク感向上剤を添加しないミルクコーヒーを定法に従って製造した。また、実施例として、ミルク感向上剤(還元麦芽糖水飴および酵母エキス)を添加したミルクコーヒーを同様に製造した。それらの配合を表8に示す。なお、実施例においては、比較例と同等の甘味にするため、水飴の添加量を低減させた。
続いて、これらのミルクコーヒーについて、実施例1(2)に記載の方法により官能試験を行い、ミルク感強度および厚味強度を評価した。ミルク感強度および厚味強度の点数化では、比較例のミルクコーヒーを比較対照(3点)とした。その結果を表8の最下段に示す。
<Example 4> Production of milk coffee As a comparative example, milk coffee to which no milk feeling improving agent was added was produced according to a conventional method. Moreover, as an example, milk coffee to which a milk feeling improving agent (reduced maltose starch syrup and yeast extract) was added was produced in the same manner. Their formulations are shown in Table 8. In addition, in the Example, in order to make sweetness equivalent to a comparative example, the addition amount of the starch syrup was reduced.
Subsequently, these milk coffees were subjected to a sensory test by the method described in Example 1 (2) to evaluate the milk feeling strength and the thickness strength. In scoring the milk feeling strength and the thickness strength, the milk coffee of the comparative example was used as a comparative control (3 points). The results are shown at the bottom of Table 8.
表8の最下段に示すように、ミルク感強度は、比較例の3点に対して、実施例では4.5点であった。また、厚味強度は、比較例の3点に対して、実施例では4点であった。すなわち、ミルク感強度および厚味強度のいずれも、比較例よりも実施例の方が顕著に大きかった。
この結果から、甘味料と酵母エキスとを有効成分とするミルク感向上剤を添加することにより、ミルク感および厚味が向上したミルクコーヒーを製造できることが明らかになった。
As shown in the lowermost row of Table 8, the milk feeling strength was 4.5 points in the example, compared to 3 points in the comparative example. Further, the thickness strength was 4 points in the example with respect to 3 points in the comparative example. That is, both the milk feeling strength and the thickness strength were significantly greater in the example than in the comparative example.
From this result, it was clarified that milk coffee with improved milk feeling and thickness can be produced by adding a milk feeling improving agent containing sweetener and yeast extract as active ingredients.
<実施例5>乳風味飲料の製造
比較例として、ミルク感向上剤を添加しない乳風味飲料を、実施例1(1)に記載の方法により製造した。また、実施例として、ミルク感向上剤(還元麦芽糖水飴および酵母エキス)を添加し、かつ、ミルク感を有する材料(脱脂粉乳)の配合量を低減した乳風味飲料を同様に製造した。それらの配合を表9に示す。なお、実施例においては、比較例と同等の甘味にするため、水飴の添加量を0とした。
続いて、これらの乳風味飲料について、実施例1(2)に記載の方法により官能試験を行い、ミルク感強度および厚味強度を評価した。ミルク感強度および厚味強度の点数化では、比較例の乳風味飲料を比較対照(3点)とした。その結果を表9の最下段に示す。
<Example 5> Manufacture of milk flavor drink As a comparative example, the milk flavor drink which does not add a milk feeling improvement agent was manufactured by the method as described in Example 1 (1). Moreover, as an Example, the milk flavor drink which added the milk feeling improvement agent (reduced maltose syrup and yeast extract) and reduced the compounding quantity of the material (skim milk powder) which has a milk feeling was manufactured similarly. Their formulation is shown in Table 9. In the examples, the addition amount of varicella was set to 0 in order to obtain sweetness equivalent to that of the comparative example.
Then, about these milk flavor drinks, the sensory test was done by the method as described in Example 1 (2), and milk feeling strength and thickness strength were evaluated. In scoring the milk feeling strength and the thickness strength, the milk flavored beverage of the comparative example was used as a comparative control (3 points). The results are shown at the bottom of Table 9.
表9の最下段に示すように、ミルク感強度は、比較例の3点に対して、実施例では4点であった。また、厚味強度は、比較例の3点に対して、実施例では3.5点であった。すなわち、ミルク感を有する材料(脱脂粉乳)の配合量を低減したにもかかわらず、ミルク感強度および厚味強度のいずれも、比較例よりも実施例の方が顕著に大きかった。
この結果から、甘味料と酵母エキスとを有効成分とするミルク感向上剤を添加することにより、ミルク感を有する材料の配合量を低減しても、低減しない場合と同等以上にミルク感および厚味が向上した乳風味飲料を製造できることが明らかになった。
As shown in the lowermost row of Table 9, the milk feeling strength was 4 points in the example compared to 3 points in the comparative example. In addition, the thickness strength was 3.5 points in the examples compared to the three points in the comparative example. That is, although the blending amount of the material having milk feeling (skimmed milk powder) was reduced, both the milk feeling strength and the thickness strength were significantly larger in the example than in the comparative example.
From these results, it was found that adding a milk feeling improver containing sweeteners and yeast extract as active ingredients reduced the milk feeling and thickness to the same level as when the amount of ingredients having a milk feeling was not reduced. It has become clear that milk flavored beverages with improved taste can be produced.
Claims (7)
The manufacturing method of Claim 6 whose addition amount of a yeast extract is more than 0.001 weight part and 1 weight part or less with respect to a total of 100 weight part of food-drinks material and a sweetener.
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KR20220137592A (en) * | 2019-12-20 | 2022-10-12 | 대상 주식회사 | Processed milk composition comprising allulose |
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