JP2018072331A5 - Endotoxin measurement system and method for quantifying endotoxin concentration - Google Patents
Endotoxin measurement system and method for quantifying endotoxin concentration Download PDFInfo
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- JP2018072331A5 JP2018072331A5 JP2017197217A JP2017197217A JP2018072331A5 JP 2018072331 A5 JP2018072331 A5 JP 2018072331A5 JP 2017197217 A JP2017197217 A JP 2017197217A JP 2017197217 A JP2017197217 A JP 2017197217A JP 2018072331 A5 JP2018072331 A5 JP 2018072331A5
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- endotoxin
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- detection chip
- current value
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- 239000002158 endotoxin Substances 0.000 title claims description 43
- 238000005259 measurement Methods 0.000 title claims description 11
- 238000001514 detection method Methods 0.000 claims description 16
- 239000000463 material Substances 0.000 claims description 8
- 230000003334 potential Effects 0.000 claims description 4
- 239000000758 substrate Substances 0.000 claims 4
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- 108010065152 Coagulase Proteins 0.000 claims 1
- 102000003712 Complement Factor B Human genes 0.000 claims 1
- 108090000056 Complement Factor B Proteins 0.000 claims 1
- 240000006614 Hericium coralloides Species 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 238000003541 multi-stage reaction Methods 0.000 claims 1
- OZAIFHULBGXAKX-UHFFFAOYSA-N precursor Substances N#CC(C)(C)N=NC(C)(C)C#N OZAIFHULBGXAKX-UHFFFAOYSA-N 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
Description
本発明は、例えば、１ＥＵ／Ｌのエンドトキシンを安定的に検出可能なエンドトキシン検出用チップを用いたエンドトキシン測定システム及びエンドトキシン濃度の定量方法に関するものである。
The present invention relates to an endotoxin measurement system using an endotoxin detection chip capable of stably detecting 1 EU/L endotoxin, and a method for quantifying endotoxin concentration .
上記目的を達成するために、本発明は、支持基材と、上記支持基材上に成形され、２つの作用極を有する電極部と、上記支持基材上に形成され、上記電極部と接続された端子部と、を少なくとも有するエンドトキシン検出用チップを用いたエンドトキシン測定システムであって、上記２つの作用電極に略同一電位を印加し、所定時間経過後、一方の電位は上記略同一電位に維持したまま、もう一方の電位を上記略同一電位とは異なる電位を印加する制御部と、を備えることを特徴とするエンドトキシン検出用チップを用いたエンドトキシン測定システムを提供する。
In order to achieve the above object, the present invention provides a supporting base material, an electrode part formed on the supporting base material and having two working electrodes, and formed on the supporting base material and connected to the electrode part. An endotoxin measuring system using an endotoxin detection chip having at least the above-mentioned terminal part , wherein substantially the same potential is applied to the two working electrodes, and after a predetermined time elapses, one potential becomes approximately the same potential. An endotoxin measurement system using an endotoxin detection chip , comprising: a control unit that applies a potential different from the substantially same potential to the other potential while maintaining the other potential .
本発明によれば、例えば、１ＥＵ／Ｌのエンドトキシンに由来して出力される電流値を安定的に検出することが可能となる。
According to the present invention, if example embodiment, it is possible to stably detected current value output derived from the endotoxin of 1 EU / L.
本発明においては、上記２つの作用極が、交互櫛形電極であることが好ましい。
In the present invention, the two working electrodes are preferably alternating comb electrodes .
本発明においては、上記異なる電位を印加する直前の電流値を基準とし、上記異なる電位を印加後、所定時間経過後の電流値を求めるものであることが好ましい。精度よくエンドトキシンを検出できるからである。
In the present invention, it is preferable that the current value immediately before the application of the different potential is used as a reference and the current value after a lapse of a predetermined time after the application of the different potential is obtained. This is because endotoxin can be detected accurately.
本発明においては、グラウンド電流を基準とし、上記異なる電位を印加後、所定時間経過後の電流値を求めるものであることが好ましい。精度よくエンドトキシンを検出できるからである。
In the present invention, it is preferable to obtain a current value after a predetermined time has elapsed after applying the different potentials with reference to the ground current . This is because endotoxin can be detected accurately.
本発明においては、上記所定時間経過後の電流値が、還元電位側電流値であることが好ましい。精度よくエンドトキシンを検出できるからである。
In the present invention, the current value after the lapse of the predetermined time is preferably the reduction potential side current value . This is because endotoxin can be detected accurately.
本発明においては、支持基材と、上記支持基材上に成形され、２つの作用極を有する電極部と、上記支持基材上に形成され、上記電極部と接続された端子部と、を少なくとも有するエンドトキシン検出用チップを用いたエンドトキシン濃度の定量方法であって、上記２つの作用電極に略同一電位を印加する工程、所定時間経過後、一方の電位は上記略同一電位に維持したまま、もう一方の電位を上記略同一電位とは異なる電位を印加する工程、を有するエンドトキシン検出用チップを用いたエンドトキシン濃度の定量方法を提供する。
In the present invention, a supporting base material, an electrode portion formed on the supporting base material and having two working electrodes, and a terminal portion formed on the supporting base material and connected to the electrode portion, A method for quantifying endotoxin concentration using at least an endotoxin detection chip, comprising the step of applying substantially the same potential to the two working electrodes, and after a predetermined time has passed, one potential is maintained at the substantially same potential, Provided is a method for quantifying endotoxin concentration using an endotoxin detection chip having a step of applying the other potential to a potential different from the substantially same potential.
本発明によれば、上記異なる電位を印加する直前の電流値を基準とし、上記異なる電位を印加後、所定時間経過後の電流値を求める工程、をさらに有するものであることが好ましい。
According to the present invention, it is preferable that the method further comprises a step of obtaining a current value after a predetermined time has elapsed after the application of the different potential, with the current value immediately before the application of the different potential as a reference.
本発明においては、グラウンド電流を基準とし、上記異なる電位を印加後、所定時間経過後の電流値を求める工程、をさらに有することが好ましい。精度よくエンドトキシン濃度を定量できるからである。 In the present invention, it is preferable that the method further includes a step of obtaining a current value after a lapse of a predetermined time after applying the different potentials with reference to the ground current . This is because the endotoxin concentration can be accurately quantified.
Claims (10)
前記支持基材上に成形され、２つの作用極を有する電極部と、
前記支持基材上に形成され、前記電極部と接続された端子部と、
を少なくとも有するエンドトキシン検出用チップを用いたエンドトキシン測定システムであって、
前記２つの作用電極に略同一電位を印加し、所定時間経過後、一方の電位は前記略同一電位に維持したまま、もう一方の電位を前記略同一電位とは異なる電位を印加する制御部と、を備えることを特徴とするエンドトキシン検出用チップを用いたエンドトキシン測定システム。 A supporting substrate,
An electrode part formed on the supporting substrate and having two working electrodes;
A terminal portion formed on the supporting base material and connected to the electrode portion,
An endotoxin measurement system using an endotoxin detection chip having at least
A control unit that applies substantially the same potential to the two working electrodes and, after a predetermined time has elapsed, maintains one potential at the substantially same potential and applies the other potential to a potential different from the substantially same potential. endotoxin measurement system using an endotoxin detection chip, characterized in that it comprises a.
前記支持基材上に成形され、２つの作用極を有する電極部と、 An electrode part formed on the supporting substrate and having two working electrodes;
前記支持基材上に形成され、前記電極部と接続された端子部と、 A terminal portion formed on the supporting base material and connected to the electrode portion,
を少なくとも有するエンドトキシン検出用チップを用いたエンドトキシン濃度の定量方法であって、 A method for quantifying an endotoxin concentration using an endotoxin detection chip having at least
前記２つの作用電極に略同一電位を印加する工程、 Applying substantially the same potential to the two working electrodes,
所定時間経過後、一方の電位は前記略同一電位に維持したまま、もう一方の電位を前記略同一電位とは異なる電位を印加する工程、 After a lapse of a predetermined time, a step of applying a potential different from the substantially same potential to the other potential while maintaining the one potential at the substantially same potential,
を有することを特徴とするエンドトキシン検出用チップを用いたエンドトキシン濃度の定量方法。 A method for quantifying endotoxin concentration using a chip for detecting endotoxin, comprising:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/JP2017/038462 WO2018079581A1 (en) | 2016-10-25 | 2017-10-25 | Endotoxin detection chip and method for quantitatively determining endotoxin concentration |
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| JP2016208519 | 2016-10-25 | ||
| JP2016208519 | 2016-10-25 |
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| JP2018072331A JP2018072331A (en) | 2018-05-10 |
| JP2018072331A5 true JP2018072331A5 (en) | 2020-08-13 |
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| JP2017197217A Pending JP2018072331A (en) | 2016-10-25 | 2017-10-10 | Endotoxin detection chip and endotoxin density quantitative method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2020184482A1 (en) | 2019-03-12 | 2020-09-17 | 国立大学法人東北大学 | Endotoxin detection device and endotoxin detection method |
| JPWO2021210669A1 (en) | 2020-04-16 | 2021-10-21 | ||
| TWI768560B (en) * | 2020-11-25 | 2022-06-21 | 五鼎生物技術股份有限公司 | Biochemical test chip |
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| JPH05281181A (en) * | 1992-03-30 | 1993-10-29 | Nippon Telegr & Teleph Corp <Ntt> | Enzyme modified electrochemical detector and its manufacture |
| JPH1164271A (en) * | 1997-08-18 | 1999-03-05 | Nagoyashi | Current amplification type oxyzen sensor |
| JP5561613B2 (en) * | 2010-12-13 | 2014-07-30 | 国立大学法人東北大学 | Endotoxin concentration detection method and endotoxin detection electrode chip |
| JP6128578B2 (en) * | 2012-09-05 | 2017-05-17 | 国立研究開発法人産業技術総合研究所 | Endotoxin concentration measuring method and endotoxin concentration measuring apparatus |
| JP6311234B2 (en) * | 2013-08-09 | 2018-04-18 | 大日本印刷株式会社 | Biosensor electrode fabric, biosensor electrode and biosensor |
| JP5916176B2 (en) * | 2014-03-11 | 2016-05-11 | 大日本印刷株式会社 | Microbial contaminant concentration detection method, electrode chip and oligopeptide |
| JP6240543B2 (en) * | 2014-03-28 | 2017-11-29 | 大日本印刷株式会社 | Electrode tip and method for quantitative determination of chemical substance |
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